Phenotypic and genotypic detection of multi drug resistant coagulase-positive Staphylococcus spp. isolates from canine pyoderma.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2024-01-01 DOI:10.22099/IJVR.2024.49531.7283

K Shobha, S N Ghodasara, D B Barad, B B Javia, P J Poshiya, D K Parasana

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引用次数: 0

Abstract

Background: Dermatological infections in dogs are challenging to treat due to antibiotic resistance, which leads to longer recovery time and the need for stronger antibiotics. Aims: This study was undertaken to determine the prevalence of antimicrobial resistance in coagulase-positive staphylococcal isolates from pyoderma infection in dogs. This study also aimed to identify isolates with methicillin-resistance and multidrug resistance.

Methods: 73 coagulase-positive staphylococci isolated from varying degrees of canine pyoderma cases. The samples were analyzed for the presence of Staphylococcus spp. using polymerase chain reaction (PCR) and resistance against antibiotics was studied by antimicrobial profile, minimum inhibitory concentration (MIC), and PCR on isolated bacteria.

Results: Out of 75 bacterial isolates identified, 73 isolates were confirmed as Staphylococcus species by PCR. A higher percentage of antibiotic resistance was observed against penicillin-G (46.27%), followed by amoxiclav (38.81%), enrofloxacin (32.84%), cefpodoxime, oxytetracycline (28.36% each), levofloxacin (26.86%), and co-trimoxazole (22.39%). 29 (49.15%) S. pseudintermedius, three (50.00%) S. schleiferi subsp. coagulans, and two (100%) S. aureus isolates exhibited multidrug resistance. However, one (1.49%) isolate (S. pseudintermedius) revealed low-level mupirocin resistance in the E-test. Also, 12 (20.34%) methicillin-resistant Staphylococcus pseudintermedius (MRSP), one (16.67%) methicillin-resistant S. schleiferi subsp. coagulans (MRSS) and one (50%) methicillin-resistant S. aureus (MRSA) were reported using PCR.

Conclusion: This study helps to understand the increased level and pattern of resistance in coagulase-positive staphylococci isolated from different types of canine pyoderma cases.

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.