Iranian journal of veterinary research最新文献

{"title":"The effects of music and darkness on radionuclide distribution during mice FDG-PET scan.","authors":"K Tanha, M Seyedabadi, H Ghadiri, M Ay","doi":"10.22099/ijvr.2024.49641.7306","DOIUrl":"10.22099/ijvr.2024.49641.7306","url":null,"abstract":"<p><strong>Background: </strong>There is growing interest in the therapeutic potential of music or light in different human disorders.</p><p><strong>Aims: </strong>This study aimed to evaluate the effects of music as well as darkness on FDG uptake in 4T1 tumor-bearing BALB/c mice using a PET scan.</p><p><strong>Methods: </strong>The music, darkness, and music plus darkness groups were subjected to either song or darkness and their combination, respectively, 30 min before the radiopharmaceutical injection until the end of the experiments. The control group was imaged in silence under ambient conditions.</p><p><strong>Results: </strong>Our results revealed that music did not significantly alter the range of tumor SUVmean, but showed a slight increase in brain SUVmean (18.2%) and about 100% increase in brain percentage of injected dose per gram (%ID/g) in <i>ex vivo</i> analysis. In contrast, heart SUVmean and heart %ID/g were approximately half those of the silence group. The muscle SUVmean and blood activity measurements showed a decrement upon music exposure. Also, results showed a significant difference in tumor-to-muscle ratio (85% increment) and brain-to-muscle ratio (105% increment) between the silence and music groups. The muscle SUVmean decreased by 50%, and tumor-to-muscle and brain-to-muscle ratios were observed to increase by 44% and 60% in the group exposed to darkness, respectively.</p><p><strong>Conclusion: </strong>Our results suggest that music and environmental factors may influence FDG uptake in small-animal PET imaging, and provide important insights into the reliability of FDG-PET imaging for music intervention research and may aid researchers in investigating the effects of music on brain changes and tissue metabolism.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"319-325"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of marine collagen and ascorbic acid on the tendon repair in the rat model: a biomechanicaland histopathological study.","authors":"B Avazzadeh, A Meimandi-Parizi, A Khodakaram-Tafti","doi":"10.22099/ijvr.2025.50639.7481","DOIUrl":"10.22099/ijvr.2025.50639.7481","url":null,"abstract":"<p><strong>Background: </strong>Tendon injuries are common and can lead to significant morbidity. Marine collagen and ascorbic acid have shown potential for promoting tendon repair and regeneration.</p><p><strong>Aims: </strong>This study investigated the effects of marine collagen, ascorbic acid, and their combination on tendon healing in a rat model over 6-week duration.</p><p><strong>Methods: </strong>Sixty adult male Wistar rats were randomized into four equal groups: control, collagen + ascorbic acid, collagen, and ascorbic acid. A controlled full-thickness transverse incision was made in the mid-portion of the Achilles tendon to induce injury, and the defects were sutured following treatment. The treatments were administered orally for 30 days. Tendon recovery was evaluated through histopathological assessments after 30-day treatment at 2-, 4-, and 6-weeks post-operation and biomechanical properties analyzed at 6 weeks.</p><p><strong>Results: </strong>Histological evaluation revealed significantly higher connective tissue and collagen accumulation in the collagen + ascorbic acid group (75-90%) compared with the collagen (50-75%), ascorbic acid (25-50%), and control groups (25-50%) (P<0.05). Biomechanical analyses showed at 6 weeks after surgery that the collagen + ascorbic acid group had significantly higher maximum force (28.6 ± 3.2 N), maximum stress (4.8 ± 0.6 MPa), work (90.4 ± 11.3 N.mm), and yield points (18.9 ± 2.1 N) compared to the collagen, ascorbic acid, and control groups (P<0.05).</p><p><strong>Conclusion: </strong>The combined supplementation of marine collagen and ascorbic acid significantly enhanced tendon healing, as evidenced by improved histopathological and biomechanical parameters. These findings suggest that this combination is a promising therapeutic strategy for tendon repair and regeneration.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 2","pages":"152-160"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of probiotic supplementation on meat and litter quality and leg health of broilers reared under high stocking density conditions.","authors":"R Rezapour, S A Hosseinian, S S Shekarforoush, S Basiri, H Haghighatkhah","doi":"10.22099/ijvr.2025.53025.7941","DOIUrl":"10.22099/ijvr.2025.53025.7941","url":null,"abstract":"<p><strong>Background: </strong>High stocking density is one of the main factors that impair the performance and welfare of broilers.</p><p><strong>Aims: </strong>This study aimed to investigate the effects of <i>Bacillus</i> and <i>Lactobacillus</i> probiotics, either alone or in combination, on meat quality, peroxide value of fat tissue, leg health, and litter quality in broilers under high stocking density.</p><p><strong>Methods: </strong>Two hundred one-day-old Cobb 500 chicks were divided randomly into five treatments (4 replicates and ten birds per replicate): Control (normal stocking density, no probiotic), HD (high stocking density, no probiotic), HDpBacil (high stocking density, <i>Bacillus</i>-based probiotic), HDpLAB (high stocking density, <i>Lactobacillus</i>-based probiotic), HDpMix (high stocking density, combined probiotic containing <i>Bacillus</i> and <i>Lactobacillus</i>). Litter quality was assessed on days 21 and 42. Footpad dermatitis was also scored on days 14, 28, and 42. Gait score, peroxide value in fat tissue, and breast and thigh meat quality were measured on day 42.</p><p><strong>Results: </strong>The HD group had significantly the highest footpad dermatitis and gait scores and the lowest litter quality levels (P<0.05). The combined probiotic improved leg health and litter quality indices (P<0.05) and reduced the water holding capacity (WHC) and peroxide value (P<0.05). While, the highest peroxide value of abdominal fat and WHC of thigh meat were in the HD group (P<0.05).</p><p><strong>Conclusion: </strong>The combination of probiotics (<i>Bacillus</i> and <i>Lactobacillus</i>) can mitigate the adverse effects of high stocking density in broilers. It can be a beneficial feed additive in broilers against highly stressful conditions.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 4","pages":"350-359"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New host and distribution records for three species of <i>Dactylogyrus</i> (Dactylogyridae: Monogenea) from the endemic Anatolian fish species, <i>Alburnus escherichii</i> Steindachner, 1897 (Teleostei, Leuciscidae) in Turkey: Occurrence in relation to seasons and host factors.","authors":"N Aydogdu","doi":"10.22099/ijvr.2025.49775.7338","DOIUrl":"10.22099/ijvr.2025.49775.7338","url":null,"abstract":"<p><strong>Background: </strong>This is the first record of dactylogyrid parasites from the endemic fish species, <i>Alburnus escherichii</i> Steindachner, 1897 in Turkey.</p><p><strong>Aims: </strong>The purpose of this study was to define the dactylogyrid specimens of <i>A. escherichii</i> from Sarısu stream.</p><p><strong>Methods: </strong>A total of 216 individuals of <i>A</i>. <i>escherichii</i> were collected from Sarısu stream between winter (February) and autumn (September) 2023 with seasonal intervals (one sample per season) and screened for the presence of dactylogyrid parasites infections.</p><p><strong>Results: </strong>It was determined that 57 <i>A. esherichii</i> were infected by one or more dactylogyrid parasite specimens (overall prevalence 26.3%). A total of 3 species of dactylogyrid parasites were identified on the gills: <i>Dactylogyrus fraternus</i> Wagener, 1909, <i>Dactylogyrus alatus</i> Linstow, 1878 and <i>Dactylogyrus minor</i> <i>Wagener, 1857.</i> <i>D. fraternus</i> was found to be the dominant parasite species in host fish species.</p><p><strong>Conclusion: </strong>To the best of our knowledge, this is the first study in the world on dactylogyrid parasites of <i>A. escherichii</i>. The host fish represents a new host record for each of the collected dactylogyrid parasite specimens. Furthermore, the present study provides the first record of <i>D. minor</i> in Turkish freshwater fish.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"27-33"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating bovine coronavirus in Indian calves: Incidence, molecular evidence, and pathological role in bovine respiratory disease complex (BRDC).","authors":"B Kamdi, R Singh, V Singh, S Singh, P Kumar, G Kashyap, K Dhama","doi":"10.22099/ijvr.2024.48820.7142","DOIUrl":"10.22099/ijvr.2024.48820.7142","url":null,"abstract":"<p><strong>Background: </strong>Bovine coronavirus (BCoV) and bacterial pathogens contribute to bovine respiratory disease complex (BRDC) in young calves. However, the role of BCoV in BRDC occurrence and site-specific respiratory pathology in India remains poorly explored.</p><p><strong>Aims: </strong>This study aimed to assess BCoV prevalence in BRDC cases.</p><p><strong>Methods: </strong>We investigated 406 weaner calves (166 cattle, 240 buffaloes) up to ≤1 year, with respiratory distress and pulmonary lesions.</p><p><strong>Results: </strong>BRDC cases exhibited 0.98% BCoV occurrence, confirmed by partial <i>N</i> gene amplification (172 bp) via RT-PCR and immunohistochemistry (IHC). Grossly, 4 BCoV positive cases showed variable degrees of consolidation of cranioventral lobes and non-collapsed caudodorsal lobes, associated with congestion and emphysema. Microscopically, the inflated sites of the lung tissue sections showed hallmark changes of interstitial pneumonia characterized by moderate infiltration with lymphocytes and increased numbers of fibroblasts in the interalveolar septa and the stroma of bronchioles and bronchi. In concomitant <i>Pasteurella multocida</i> infected cases, cranioventral lobes exhibited suppurative bronchopneumonia with neutrophilic exudate. The above lesions were well colocalized with BCoV antigen in the epithelial cells and in the debris of the lumen of the alveoli and the bronchi/bronchioles. The sequence comparison of the 172 bp amplicon with the published BCoV <i>N</i> gene showed close relatedness.</p><p><strong>Conclusion: </strong>The present study implicated BCoV as a component of BRDC in India that should be considered in the diagnosis of BRDC outbreaks.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"361-367"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune efficacy of <i>oprH</i> chitosan nanoparticle DNA vaccine against <i>Pseudomonas aeruginosa</i>.","authors":"G Qiang, L Yajing, Z Shiji, T Jiayu, L Jingwen","doi":"10.22099/ijvr.2024.49964.7371","DOIUrl":"10.22099/ijvr.2024.49964.7371","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> is a zoonotic pathogen that poses a threat to human and animal health. However, no vaccine exists for controlling this bacterium.</p><p><strong>Aims: </strong>This study aimed to evaluate the immune efficacy of a chitosan nanoparticle DNA vaccine of the <i>oprH</i> gene from <i>P. aeruginosa</i>.</p><p><strong>Methods: </strong>The naked DNA vaccine based on the <i>oprH</i> gene of <i>P. aeruginosa</i> was constructed. Then, the chitosan nanoparticle DNA vaccine of the <i>oprH</i> gene was prepared and the shape, size, encapsulation efficiency, stability, and ability of anti-DNA enzyme degradation were detected. Chickens were divided into five groups, namely the naked DNA vaccine group (poprH group), chitosan nanoparticle DNA vaccine group (CpoprH group), outer membrane protein vaccine group (OMP group), inactive vaccine group, and PBS group. After being vaccinated with corresponding vaccines, the levels of serum antibodies, lymphocyte proliferation assays, interferon-γ (IFN-γ), interleukin-2 (IL-2), and interleukin-4 (IL-4) concentrations were detected. Groups of chickens were challenged with live virulent <i>P. aeruginosa</i> 2 weeks after the last vaccination and the survival numbers were counted until day 15 post challenge. Then, the protective rates were calculated.</p><p><strong>Results: </strong>The particle size of the chitosan nanoparticle DNA vaccine was approximately 200 nm and close to spherical; the encapsulation efficiency was 95.88%, and it could effectively resist degradation by DNase. Following vaccination, serum antibodies, stimulation index (SI) value, and concentrations of IFN-γ, IL-2, and IL-4 in chickens immunized with the chitosan nanoparticle DNA vaccine were significantly higher than those that were vaccinated with the naked DNA vaccine (P<0.05). The protective rates of poprH, CoprH, OMP vaccine, and inactive vaccine groups were 55%, 75%, 75%, and 90%, respectively.</p><p><strong>Conclusion: </strong>Chitosan could significantly enhance the immune response and protection provided by the naked DNA vaccine of the <i>oprH</i> gene.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"353-360"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report on the dual infection with emerging viruses, Tilapia Lake Virus (TiLV) and Tilapia Parvovirus (TiPV) in the wild Nile Tilapia (<i>Oreochromis niloticus</i>) from Pulicat Lagoon, Tamil Nadu, India.","authors":"S Porselvan, A Uma, D Kaviarasu, P Pavinkumar, V Santhiya","doi":"10.22099/ijvr.2025.51313.7618","DOIUrl":"10.22099/ijvr.2025.51313.7618","url":null,"abstract":"<p><strong>Background: </strong>Pulicat Lagoon, Tamil Nadu, India, is a major fish landing site supporting the livelihoods of local fishing communities. Despite its ecological and economic importance, information on viral diseases affecting its fish populations remains limited.</p><p><strong>Aims: </strong>This study aimed to investigate the prevalence and seasonal occurrence of viral infections in the tilapia population of Pulicat Lagoon.</p><p><strong>Methods: </strong>Active surveillance was conducted from November 2023 to July 2024. Collected tilapia samples were screened for viral pathogens using PCR, followed by sequencing and histopathological analysis for confirmation.</p><p><strong>Results: </strong>Among 314 tilapia samples collected and analyzed, 54 (17.19%) tested positive for viral infections, including Tilapia Lake Virus (TiLV) (13.69%), Tilapia parvovirus (TiPV) (9.25%), and co-infections with both TiLV and TiPV (6.68%). PCR-positive samples were predominantly detected from January to March 2024, indicating a higher prevalence during the post-monsoon season. Sequencing and histopathological examinations confirmed the presence of these viruses.</p><p><strong>Conclusion: </strong>The findings of this study on the detection of TiLV and TiPV in tilapia populations from Pulicat Lagoon, located in Tiruvallur District, Tamil Nadu, India, can play a crucial role in raising awareness among aquaculture farmers in nearby regions to implement effective biosecurity measures, as well as among researchers and local fishing communities who rely on the lagoon's fish stocks for their livelihoods.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 3","pages":"263-269"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13378043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of neonatal calves fed kitchen herbs and probiotics dissolved in whole milk up to weaning age.","authors":"S Rai, S Mandal, T K Dutta, A Chatterjee, M Mondal, M Karunakaran","doi":"10.22099/ijvr.2025.49907.7363","DOIUrl":"10.22099/ijvr.2025.49907.7363","url":null,"abstract":"<p><strong>Background: </strong>Diarrhea is widespread in neonatal calves, often treated with antibiotics. However, prolonged use of these antibiotics may promote problems of antimicrobial resistance.</p><p><strong>Aims: </strong>The experiment was carried out to determine the effect of feeding cinnamon, turmeric, carom seed powder, and probiotics fortified milk on the health and growth performance of Jersey crossbred calves.</p><p><strong>Methods: </strong>A complete randomized block design was applied with 4 treatments of 10 calves in each experimental unit. All experimental groups except the control group were offered herbs, probiotics and a mixture of both, respectively for 3 months.</p><p><strong>Results: </strong>Calves fed herb-probiotic mixture had significantly higher dry matter intake (DMI) from concentrates (P<0.05, 0.72 kg), dry matter intake (DMI) per 100 kg body weight (P<0.05, 2.30 kg) and had better fecal scores (P<0.05, 1.19±0.07) when compared to the other treatment groups. Simultaneously, there was an increase in the Lactobacillus sp. (P<0.05, 7.58±0.44 CFU/g) with markedly reduced E. coli counts (P<0.01, 4.93±0.41 CFU/g) when the herb-probiotics combination was fed as compared to the control calves. Also, the average duration of illness was lowest in the herb-probiotic group (3.38±0.59 days), with higher serum total protein levels (P<0.05, 5.33±0.15 g/dL).</p><p><strong>Conclusion: </strong>It can be concluded that feeding herb-probiotic mixtures reduced calf diarrhea and improved dry matter intake in calves.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 2","pages":"179-188"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of carboxymethyl dextran-asparaginase in NALM-6 cell apoptosis and autophagy.","authors":"M Azizian, G H Tamaddon, M Ashrafi, M Chahardahcherik, F Gharechahi","doi":"10.22099/ijvr.2024.49166.7208","DOIUrl":"10.22099/ijvr.2024.49166.7208","url":null,"abstract":"<p><strong>Background: </strong>Acute lymphoblastic leukemia (ALL) is a malignant disorder in both humans and animals. L-asparaginase (L-ASNase) has limitations as a chemotherapy agent due to adverse effects and low serum stability. In a previous study, L-ASNase was chemically modified with carboxymethyl dextran to enhance its properties.</p><p><strong>Aims: </strong>This study aimed to validate the potential of these modifications using the NALM-6 cell line.</p><p><strong>Methods: </strong>NALM-6 cells were cultured and treated with various concentrations, including 0 IU/ml as negative control, 0.5, 1, 1.5, and 2 IU/ml of modified L-ASNase and L-ASNase. The optimal concentration was determined at specific intervals, and viability and metabolic activity were assessed through Trypan blue and MTT tests. Flow cytometry, using Annexin V/PI staining, was employed to evaluate apoptosis. Real-time RT-PCR techniques were used to determine changes in the expression of the <i>ATG2B</i> and <i>LC3-II</i> genes (important genes in autophagy), with data analysis conducted using PRISM software.</p><p><strong>Results: </strong>The modified L-ASNase reduced the viability of NALM-6 cells and induced higher levels of apoptosis (P=0.001). Interestingly, the modified enzyme had a lesser impact on autophagy, which is important for avoiding treatment resistance.</p><p><strong>Conclusion: </strong>The modified L-ASNase showed enhanced effectiveness in reducing the viability of NALM-6 cells and induced higher levels of apoptosis. Interestingly, the modified enzyme had a lesser effect on autophagy, which is important as excessive autophagy can lead to treatment resistance. These findings suggest that the modified L-ASNase may have the potential to be a more effective chemotherapeutic agent for ALL treatments.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"344-352"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of <i>Salmonella</i>, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> O157:H7 in goat and sheep milk from four rural and tribal regions, Fars province, Iran.","authors":"M Sayyadi, S Rahmdel, S Pashangeh, S Hosseinzadeh, F Fani, M Zare, S S Shekarforoush","doi":"10.22099/ijvr.2025.50038.7379","DOIUrl":"10.22099/ijvr.2025.50038.7379","url":null,"abstract":"<p><strong>Background: </strong>Raw sheep and goat milk may harbor major foodborne pathogens of public health concern, including <i>Salmonella</i>, <i>Listeria monocytogenes</i>, and <i>E. coli</i> O157:H7. The rising issue of antimicrobial resistance further underscores the need for monitoring these contaminants.</p><p><strong>Aims: </strong>This research paper describes a survey of the frequency and antimicrobial susceptibility of <i>Salmonella</i>, <i>Listeria monocytogenes</i>, and <i>Escherichia coli</i> O157:H7 in milk samples from goat and sheep herds, located in Fars province, Iran.</p><p><strong>Methods: </strong>A total number of 110 milk samples from 50 goat and sheep herds located in tribal and rural regions were investigated for pathogenic organisms. The antimicrobial susceptibility of the isolates was determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.</p><p><strong>Results: </strong>None of the samples showed the presence of <i>Salmonella</i>. Four samples (3.6%) from three rural herds contained <i>L. monocytogenes</i>, all positive for the species-specific virulence gene <i>hlyA</i>. <i>E.</i> <i>coli</i> O157:H7 was detected in five milk samples (4.5%) belonging to four rural herds. The four virulence genes (<i>stx</i> ₁, <i>stx</i> ₂, <i>eae</i>, and e<i>hlyA</i>), and two serotyping genes (<i>rfbE</i>, and <i>fliC</i>) were found in all <i>E.</i> <i>coli</i> O157:H7 isolates. The somatic cell count of contaminated samples was lower than the US legal limit of 1.0 × 10⁶ cells/ml; milk contamination was not, therefore, related to the mastitis. All <i>L. monocytogenes</i> isolates were resistant to penicillin, ampicillin, and trimethoprim-sulfamethoxazole. The <i>E. coli</i> O157:H7 isolates exhibited full or intermediate resistance to ampicillin, cefixime, and chloramphenicol, but they were susceptible to gentamicin, kanamycin, streptomycin, tetracycline, ciprofloxacin, trimethoprim-sulfamethoxazole, and trimethoprim.</p><p><strong>Conclusion: </strong>Despite the low occurrence, the presence of foodborne pathogens in raw milk reinforces the need to control the sale and consumption of raw milk dairy products in rural and tribal regions where goat and sheep farming is of great economic importance.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 3","pages":"240-247"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13378030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148578783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}