Iranian journal of veterinary research最新文献

{"title":"Genetic and phylogenetic evaluations of <i>Schistosoma turkestanicum</i> isolated from goats in Western Iran.","authors":"H Nayebzadeh, N Nazifi, H R Shokrani, Sh Yavari","doi":"10.22099/ijvr.2024.49135.7203","DOIUrl":"10.22099/ijvr.2024.49135.7203","url":null,"abstract":"<p><strong>Background: </strong>Schistosomiasis, a zoonotic parasitic infection, poses significant challenges to the animal husbandry industry, leading to substantial economic losses. Despite its impact, there is limited data on the genotypes of Schistosoma (=Orientobilharzia) turkestanicum in Iran.</p><p><strong>Aims: </strong>The present study aimed to evaluate the phylogenetic relationships of Schistosoma turkestanicum isolated from goats by analyzing the mitochondrial cytochrome C oxidase subunit 1 (Cox1) gene sequence in Lorestan province, located in Western Iran.</p><p><strong>Methods: </strong>DNA extraction was performed on 20 male parasitic worms, and the mitochondrial <i>Cox1</i> gene was amplified using the polymerase chain reaction (PCR) process, and sent for sequencing after purification by ethanol alcohol. The sequences were trimmed using CLC Main Workbench software. Phylogenetic analysis was conducted using the Neighbor-Joining method with 1000 bootstrap replicates in MEGA6 software to assess evolutionary relationships between the Cox1 gene sequence obtained in this study (GenBank accession No. PP627151) and various S. turkestanicum sequences obtained from the National Center for Biotechnology Information (NCBI). Additionally, this software was also used to plot the genetic distance matrix (nucleotide differences and similarities).</p><p><strong>Results: </strong>Phylogenetic analysis confirmed that the parasite isolated in this study was S. turkestanicum.</p><p><strong>Conclusion: </strong>The findings indicate that the S. turkestanicum lineage identified in this study is closely related to those found in Mazandaran province, Iran, as well as to African Schistosoma species.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"273-278"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of human smartwatch and transmittance pulse oximetry for evaluating peripheral oxygen saturation in anesthetized dogs.","authors":"M G Senocak, S Okur, U Ersoz, L E Yanmaz, Y Kocaman, F Turgut, A G Bedir, O T Orhun","doi":"10.22099/IJVR.2024.47628.6884","DOIUrl":"https://doi.org/10.22099/IJVR.2024.47628.6884","url":null,"abstract":"<p><strong>Background: </strong>Pulse oximetry is a valuable tool for monitoring animals during anesthesia and assessing the adequacy of administered oxygen therapy.</p><p><strong>Aims: </strong>To compare the pulse oximeter readings obtained by the Garmin Fenix 5X plus (GF5Xp) smartwatch and transmittance pulse oximetry (TPO) in anesthetized dogs.</p><p><strong>Methods: </strong>Twelve clinical canine patients requiring anesthesia for castration were prospectively enrolled in this study. The animals were premedicated with intramuscular dexmedetomidine at a dose of 5 µg/kg. Anesthesia was induced through intravenous administration of propofol and maintained using sevoflurane. The arterial hemoglobin oxygen saturation (SpO₂) readings obtained from the tongue using TPO (238 readings) were compared with measurements taken over the lateral side of the tibia using a GF5Xp smart wearable device (238 readings). This comparison was performed using a Bland-Altman plot, where the differences (%) between the methods were plotted against their mean SpO₂ (Gold standard - Device), and the limits of agreement were represented as the mean ± 1.96 times the standard deviation.</p><p><strong>Results: </strong>The SpO₂ levels in dogs were overestimated by the GF5Xp relative to the readings obtained by the TPO, with the bias of -0.3% (95% CI: -3.1%-2.5%).</p><p><strong>Conclusion: </strong>GF5Xp may be interchangeable with TPO in dogs. Further studies are required to validate the accuracy of the GF5Xp in non-anesthetized dogs or dogs outside the physiological range.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"166-169"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-farm epidemiology and phylogenetic evaluation of methicillin and beta-lactam-resistant <i>Staphylococcus aureus</i> isolated from dairy cattle and buffaloes with endometritis.","authors":"P Bakht, M Ijaz, M Z Iqbal, H B Aslam, A Rehman","doi":"10.22099/IJVR.2024.48563.7095","DOIUrl":"https://doi.org/10.22099/IJVR.2024.48563.7095","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a potential emerging and prevailing multidrug-resistant (MDR) pathogen involved in bovine endometritis.</p><p><strong>Aims: </strong>Present research evaluated the prevalence and molecular characterization of methicillin-resistant <i>S. aureus</i> (MRSA) and beta-lactam resistant <i>S. aureus</i> (BRSA) and also analyzed the associated risk factors with endometritis along with antibiotic resistance patterns.</p><p><strong>Methods: </strong>A total of 384 uterine and vaginal fluid samples were collected from the adult dairy cattle and buffaloes showing the clinical signs of endometritis including foul-smelling vaginal discharge, fever, enlarged and thickened uterine horns on rectal palpation, and confirmation by ultrasonography findings. The collected samples were subjected to standard microbiological methods for the detection of <i>S. aureus</i>. The confirmed isolates were further subjected to the Kirby-Bauer disc diffusion test and the detection of the <i>mecA</i> and <i>blaZ</i> genes for the confirmation of MRSA and BRSA.</p><p><strong>Results: </strong>Study found an overall prevalence of 17.96% for <i>S. aureus</i> from bovine endometritis cases. Among <i>S. aureus</i> isolates, 50.72% and 37.68% isolates were confirmed MRSA while BRSA was found as 36.23% and 18.84%, based on phenotypic and genotypic methods, respectively. Phylogenetic analysis indicated the possibility of pathogen transmission within and between livestock animals. Risk factor analysis showed that the breed of animal, visible discharge from vagina, lactation number, insemination procedure, and calving place showed significant (P<0.05) association with <i>S. aureus</i>-associated endometritis. Antimicrobial susceptibility testing of study isolates showed the resistance to various commonly used antibiotics.</p><p><strong>Conclusion: </strong>The study concluded that <i>S. aureus</i> is found in 17.96% of bovines affected with endometritis and require further intensive research to elucidate the farm economic losses.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"98-106"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of quorum sensing and biofilm formation on antimicrobial resistance and virulence of XDR and MDR <i>Pseudomonas aeruginosa</i> in laying chickens.","authors":"N H Eidaroos, H I Eid, S A A Nasef, G H Mansour, R M El-Tarabili","doi":"10.22099/IJVR.2024.47975.6969","DOIUrl":"https://doi.org/10.22099/IJVR.2024.47975.6969","url":null,"abstract":"<p><strong>Background: </strong><i>Pseudomonas aeruginosa</i> plays a major influence on poultry outbreaks. Several factors may contribute to its pathogenicity.</p><p><strong>Aims: </strong>This study aimed to investigate the prevalence of <i>P. aeruginosa</i> infection among layer chickens with phenotypic and genotypic characterization of the isolates.</p><p><strong>Methods: </strong>Samples (n=160) were collected from respiratory distressed layer chickens according to the lesion and bacteriologically examined for isolation of <i>P. aeruginosa</i> from Sharkia province, Egypt. The antimicrobial sensitivity was performed against 18 antimicrobial agents. A qualitative assessment of biofilm production was performed using the Tube method. The isolates were genetically examined for confirmation, detection of quorum sensing genes, virulence genes, and biofilm production genes by conventional PCR.</p><p><strong>Results: </strong><i>P. aeruginosa</i> was isolated from 25% of the samples. Moreover, 95% of the isolates were extensively drug-resistant (XDR) with multiple antibiotic resistance indices (MARI) of 0.67 to 0.83. A total of 38 isolates were able to produce biofilm with different degrees. PCR of <i>16S rRNA</i> (<i>P. aeruginosa</i>) and <i>opr</i>L genes confirmed the existence of <i>P. aeruginosa</i> isolates. For quorum sensing genes, <i>lasI</i> and <i>lasR</i> were successfully amplified at 100% and 89.5%, respectively. For virulence genes, <i>tox</i>A and <i>exo</i>U were amplified by a percentage of 78.9%, while the <i>higBA</i> gene was in 100% of the isolates. <i>ppr</i>A and <i>ppr</i>B genes were amplified at 100% and 89.5%, respectively. For biofilm genes, <i>psl</i>A, <i>fli</i>C, and <i>pel</i>A were amplified in 100%, 84.2%, and 10.5%, respectively.</p><p><strong>Conclusion: </strong>A strong correlation between quorum sensing genes, biofilm genes, and virulence genes was detected. Further, biofilm production increases the resistance of the isolates to antimicrobial agents.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"125-134"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary incorporation of magnetic bentonite nanocomposite: impacts on <i>in vitro</i> fermentation pattern, nutrient digestibility, and growth performance of Baluchi male lambs.","authors":"E Ibrahimi Khoram Abadi, S Heydari, M Kazemi","doi":"10.22099/IJVR.2024.47753.6919","DOIUrl":"10.22099/IJVR.2024.47753.6919","url":null,"abstract":"<p><strong>Background: </strong>Incorporation of bentonite into the diets of ruminants can be helpful to maximize their performance. Modifying the structure of bentonite to nano and nanocomposite has improved their chemical stability and physicochemical properties, enhancing adsorption, absorption, and cation exchange capacity. <b>Aims:</b> This study aimed to assess the effect of magnetic bentonite nanocomposite (MBNC) on <i>in vivo</i> and <i>in vitro</i> fermentation process patterns, nutrient digestibility, and growth performance of Baluchi male lambs.</p><p><strong>Methods: </strong>Effects of control (basal diet), natural bentonite (NB) (10 g/kg dry matter (DM)), processed bentonite (PB) (5 and 10 g/kg DM basal diet), and MBNC (5 and 10 g/kg DM basal diet) on gas production (GP), and the fermentation process were determined using <i>in vitro</i> GP technique. For the <i>in vivo</i> experiment, 20 Baluchi male lambs were used with 4 experimental treatments: control, NB (5 g/kg DM), PB (5 g/kg DM), and MBNC (5 g/kg DM) and 5 replications in a completely randomized design for 60 consecutive days.</p><p><strong>Results: </strong>The potential for GP and its fractional rates were significantly decreased and increased in MBNC, respectively (P<0.01). The lowest cumulative GP, and CH₄ yield were observed in MBNC (P<0.05). <i>In vitro</i>, DM and organic matter (OM) digestibility and all fermentation parameters increased with the addition of two levels of MBNC to the culture medium (P<0.01). Except for feed conversion ratio (FCR), other growth performance parameters increased with the addition of MBNC to the diet (P<0.01). The ruminal pH, total volatile fatty acids (TVFA), acetate, and propionate significantly increased when MBNC incorporated to the diet (P<0.01). The NH₃-N (P<0.001) was significantly decreased in MBNC. The bentonite supplementation decreased acetate to propionate (P=0.001) compared to the control.</p><p><strong>Conclusion: </strong>Adding MBNC at the 5 g/kg diet DM level can be used as a useful supplement to optimize rumen fermentation pattern, reduce methane production, and increase lamb performance.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"16-24"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of season on histoarchitecture of pineal gland in buffalo (<i>Bubalus bubalis</i>).","authors":"A Sharma, V Kumar, V K Singh, T K Patbandha, Y L Vyas","doi":"10.22099/IJVR.2024.48516.7084","DOIUrl":"10.22099/IJVR.2024.48516.7084","url":null,"abstract":"<p><strong>Background: </strong>The photoperiod and other seasonal variations are the key factors that affect reproduction and production of the animals. The pineal gland secretes melatonin hormone that affects several physiological functions of the body during different seasons.</p><p><strong>Aims: </strong>The present study was conducted to study the histoarchitectural and micrometrical changes in the pineal gland of buffalo (<i>Bubalus bubalis</i>) during different seasons of the year.</p><p><strong>Methods: </strong>Pineal glands of 30 adult female Jaffarabadi buffaloes were collected from the slaughterhouse during the winter, summer, and rainy seasons. Samples were processed by standard histological procedures and stained with various stains for histological and micrometrical observations.</p><p><strong>Results: </strong>The pinealocytes constituted a major cellular portion of pineal parenchyma. The pinealocyte nuclei were lightly stained and more euchromatic during the winter season whereas darkly stained and slightly heterochromatic during summer. The calcium deposits occupied a larger area of pineal parenchyma during the summer as compared to the winter season. The pinealocyte density, the nuclear diameter of pinealocytes, and the number of argyrophilic nucleolar organizer regions (AgNOR) were highest during the winter season as compared to the summer and rainy seasons.</p><p><strong>Conclusion: </strong>The present study shows the influence of season on the histoarchitecture and histometry of the pineal gland of buffalo and indicated higher pineal activity during the winter season in this species.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"54-61"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first comprehensive study on isolation and genetic characterization of canine parvoviruses from dogs in Mizoram, India reveals the emergence of CPV-2c.","authors":"P Chakraborty, S K Behera, M H Lalhriatchhungi, P Roychoudhury, L Maibam, P Behera, J K Chaudhary, H Prasad, J B Rajesh, M Lalhmangaihzuali, H C J Mary, L H Lalrosanga, M S Dawngliana, V Chander, M Kumar","doi":"10.22099/ijvr.2024.49573.7295","DOIUrl":"10.22099/ijvr.2024.49573.7295","url":null,"abstract":"<p><strong>Background: </strong>Canine parvovirus type-2 (CPV-2) is a highly contagious enteric pathogen of puppies with worldwide distribution.</p><p><strong>Aims: </strong>Molecular epidemiology, genetic characterization, phylogenetic analysis, and isolation of the CPV-2 virus from clinically affected dogs in Mizoram, India over eight years.</p><p><strong>Methods: </strong>A total of 202 samples (199 fecal samples, 2 vomita, and 1 tissue sample) were screened by PCR assay.</p><p><strong>Results: </strong>103 out of 202 samples (50.99%) tested positive. Of the 103 positive samples, 83 samples were cloned and sequenced. Sequence analysis showed CPV-2c as the predominant variant (63.85%) followed by the 2a variant (26.5%), 2b (8.43%), and FPV (1.2%). Phylogenetic analyses of the CPV-2c sequences formed separate clusters and were ancestrally related to Japanese, Chinese, and Italian 2c sequences. Similarly, 2a isolates formed separate clusters under different clades and were ancestrally related to Indian, Singaporean, Japanese, Uruguayan, and Chinese 2a isolates. 2b isolates formed a single cluster with the Chinese 2b isolate. FPV isolate clustered with North American FPV. Both synonymous and non-synonymous mutations (unique to this study) were evident in all the types of CPV-2s indicative of active evolution with regional variation. In the cell culture medium, CPV-2 showed cytopathogenic effects at the third passage level.</p><p><strong>Conclusion: </strong>The study, the first in-depth report on CPV-2, showed a shift towards CPV-2c as the predominant variant in Mizoram. This variant clustered separately from current vaccine strains, highlighting the need for extensive epidemiological surveillance to better understand viral phylogenomics and evaluate current vaccine efficacy.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"261-272"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A report of coagulase-negative <i>Staphylococci</i> from clinically incurable cases of bovine mastitis: prevalence, biofilm formation, and resistance profile.","authors":"M M Elhaig, R M El-Tarabili, A Wahdan","doi":"10.22099/ijvr.2024.49819.7347","DOIUrl":"10.22099/ijvr.2024.49819.7347","url":null,"abstract":"<p><strong>Background: </strong>Given the abuse of broad-spectrum agents in the treatment of clinical bovine mastitis, coagulase-negative <i>Staphylococci</i> (CNS) have emerged to be of clinical and epidemiological significance.</p><p><strong>Aims: </strong>The study aimed to identify CNS and <i>Staphylococcus aureus</i> in incurable clinical mastitis in 50 cattle and 90 buffaloes, determine antibiotic resistance profile, and biofilm-forming ability of CNS and <i>S. aureus</i> isolates.</p><p><strong>Methods: </strong>140 milk samples were collected from four villages in Sharkia, Egypt, for bacteriological isolation and molecular investigations.</p><p><strong>Results: </strong>Forty-nine <i>Staphylococcus</i> isolates were identified, including 11 CNS and 38 coagulase-positive <i>S. aureus</i>. The most recorded CNS strains were <i>S. epidemidis</i> (3), <i>S. simulans</i> (2), <i>S. hominis</i> (2), <i>S. chromogen</i> (2), <i>S. xylosus</i> (1), and <i>S. warneri</i> (1). A 63.2% of <i>S. aureus</i> and 27.3% of CNS isolates showed the ability to form biofilm, which was confirmed by <i>ica</i> PCR. <i>S. epidemidis</i> and <i>S. chromogen</i> were extensively drug-resistant, and most <i>S. aureus</i> isolates showed multidrug resistance (MDR). The proportion of methicillin-resistant was lower among <i>S. aureus</i> (84.2%), compared with CNS (90.9%).</p><p><strong>Conclusion: </strong>CNS present a challenge due to their uprising resistance compared with <i>S. aureus</i>. The appearance of CNS-MDR strains carrying <i>ica</i> gene leads to treatment protocol failure on bovine farms and improper control of bovine mastitis.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"279-284"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of maedi-visna virus in different organs of the spontaneously affected small ruminants in India.","authors":"Sh Valecha, H S Yadav, N Roopa, S D Vinaykumar, M Bhardwaj, V Singh, P Kumar","doi":"10.22099/IJVR.2024.47429.6844","DOIUrl":"10.22099/IJVR.2024.47429.6844","url":null,"abstract":"<p><strong>Background: </strong>Maedi-visna (MV) is a small ruminant lentiviral (SRLV) disease affecting sheep and goats, and causes pathological alterations in various organs including lungs, pulmonary lymph nodes, mammary glands, joints, and CNS. <b>Aims:</b> Present study was focused to detect the MV virus (MVV) nucleic acid and MVV p28 antigen in different organs of the spontaneously MVV affected sheep and goats.</p><p><strong>Methods: </strong>Total of 657 samples were collected from sheep and goats (169 blood, 136 lungs, 96 pulmonary lymph nodes, 74 brain, 54 mammary gland, 78 joints, and 50 spleen) and screened for MVV nucleic acid using nested PCR assay. Serum samples were screened for SRLV antibodies by cELISA. Immunolocalization of MVV was demonstrated by using the polyclonal antibody against p28 antigen by immunohistochemistry in lungs, lymph nodes, mammary glands, and joint tissues.</p><p><strong>Results: </strong>Out of 657 samples, 10.7% (70) were found positive for MVV. Among different organs, lungs showed highest positivity (25.7%) followed by mammary glands (14.8%), blood (9.5%), joint tissues (7.7%), brain (5.4%), and pulmonary lymph node (1.0%). SRLV antibodies were detected in 29.2% of the serum samples of both sheep and goats by cELISA. MVV p28 antigen immunostaining was observed in lungs, lymph nodes, mammary glands, and joint tissues. However, the presence of MVV p28 antigen could not be demonstrated in the brain tissues.</p><p><strong>Conclusion: </strong>The highest positivity of MVV in lung tissues indicated higher predilection of the virus in the pulmonary tissue.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"41-47"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penile hemangiosarcoma as a cause of stranguria in a dog: clinical presentation, imaging findings, treatment and outcome.","authors":"A Pierini, C Picchi, G Pisani, D Binanti, A Carli, F Rossi, M C Criscuolo","doi":"10.22099/IJVR.2024.48685.7113","DOIUrl":"10.22099/IJVR.2024.48685.7113","url":null,"abstract":"<p><strong>Background: </strong>Penile tumors are rare in dogs and only single case reports or small case series have been reported.</p><p><strong>Case description: </strong>An 11-year-old, cross-breed dog was presented for a two-week history of stranguria. At physical examination, a subcutaneous swelling of the penis was detected. Abdominal radiographs, ultrasonography, and CT showed a subcutaneous penile mass involving the penile urethra and <i>bulbus glandis</i> associated with marked lysis of the <i>os penis</i>. Histological features along with the neoplastic cell positivity to CD31 and FVIII immunohistochemical markers warranted a final diagnosis of penile hemangiosarcoma.</p><p><strong>Findings/treatment and outcome: </strong>The dog was treated with amputation of the penis, scrotal urethrostomy, and five adjuvant doses of doxorubicin along with thalidomide. Cutaneous and omental metastases were found 235 days after surgery. The dog was euthanized at 296 days due to bone and pulmonary metastasis.</p><p><strong>Conclusion: </strong>Penile hemangiosarcoma seems to share the same aggressive behavior with other hemangiosarcomas seen in other anatomical locations. Therefore, surgery and chemotherapy may improve survival time in dogs with penile hemangiosarcoma as well.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"74-78"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}