Iranian journal of veterinary research最新文献

{"title":"Comparing CIDR with progesterone injections and eCG with human recombinant FSH for synchronizing estrous cycle in ewes.","authors":"A Alijani, A Niasari-Naslaji, N Sayyah, M Baninajar, M Ganjkhanlou","doi":"10.22099/IJVR.2024.49344.7239","DOIUrl":"10.22099/IJVR.2024.49344.7239","url":null,"abstract":"<p><strong>Background: </strong>It is desirable in estrus synchronization in sheep to avoid intravaginal devices and to shorten the program from 14 to 6 days. Moreover, replacement of eCG with safe, cheap, and efficient gonadotropin is in worldwide demand.</p><p><strong>Aims: </strong>This study investigates the possibility of replacing eCG with human recombinant FSH (hrFSH) and CIDR with progesterone injections for estrous synchronization in ewes.</p><p><strong>Methods: </strong>Assaf and Lacaune ewes (n=170) were divided into two groups and synchronized with either progesterone injections for 6 days or CIDR for 14 days. Ewes assigned in the injection group, received progesterone (37.5 mg; SC) and GnRH analogue (7.5 µg Alarelin acetate; IM) on day 0 of the experiment. On days 3 and 6, ewes received 25 and 12.5 mg progesterone (SC), respectively. On day 6, ewes in both groups received prostaglandin F₂α (250 µg Cloprostenol; IM), and were divided into two subgroups to receive either hrFSH (75 IU Follitropin alfa; SC) or eCG (400 IU; IM). On day 7, fertile rams were introduced to ewes for 21 days. Data were analyzed using GLM and Glimmix.</p><p><strong>Results: </strong>There was no difference in the respective lambing rates, prolificacy, and fecundity between CIDR (71.1, 1.63, and 1.16%) and injections (66.7, 1.55, and 1.03%); between eCG (71.4, 1.60, and 1.14%), and hrFSH (66.3, 1.58, and 1.05%, P>0.05).</p><p><strong>Conclusion: </strong>In conclusion, 6-day progesterone injection-based protocol produced similar results to 14-day CIDR program and hrFSH could be an effective alternative for eCG during estrus synchronization in ewes.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"3-7"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bovine and human follicular fluid on semen quality of fresh and frozen-thawed semen in dual purpose Simmental (Fleckvieh) bulls: a new method of adding follicular fluid to bull semen.","authors":"A Khaki, A Nourian, S Alian Samakkhah","doi":"10.22099/ijvr.2024.49943.7369","DOIUrl":"10.22099/ijvr.2024.49943.7369","url":null,"abstract":"<p><strong>Background: </strong>Follicular fluid (FF) is a biological fluid that contains many compounds such as proteins, hormones, metabolites, antioxidants, <i>etc</i>.</p><p><strong>Aims: </strong>The purpose of this research is to investigate the effects of adding cattle and human follicular fluid on bulls' semen quality during the freezing process.</p><p><strong>Methods: </strong>Semen sampling from 12 Simmental bulls was performed in a period of 3 months (36 ejaculations with three ejaculates per bull). Each ejaculate was divided into four equal portions. Three portions were used for control (C) (semen without follicular fluid), semen containing human follicular fluid (HFF), semen containing cow follicular fluid (CFF). Another part of semen was also used to prepare seminal plasma. Sperm quality assessment was performed on fresh and thawed frozen (immediately after thawing, 1 and 2 h after thawing) semen.</p><p><strong>Results: </strong>Adding human follicular fluid to the bulls' semen can slow down the process of reducing sperm motility and can delay the increase in the percentage of dead sperm in frozen-thawed semen.</p><p><strong>Conclusion: </strong>The human follicular fluid maintains the viability and motility of bull sperms better than the control and bovine follicular fluid. The possible effect of human follicular fluid on the metabolism of sperms during the process of freezing and thawing needs to be clarified in future studies.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"233-241"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of propolis supplementation during cryopreservation of ram semen.","authors":"I H Güngör, Ş Özer Kaya, S Dayan Cinkara, A Çakır Cihangiroğlu, E Kaya, T C Acısu, F Erdem Erişir, Ö Yılmaz, S Yılmaz, S Gür, M Sönmez, G Türk","doi":"10.22099/ijvr.2024.49410.7253","DOIUrl":"10.22099/ijvr.2024.49410.7253","url":null,"abstract":"<p><strong>Background: </strong>Cryopreservation of ram semen is a very challenging process. Loss of motility during freezing does not allow artificial insemination of rams.</p><p><strong>Aims: </strong>This study aimed to determine whether the inclusion of liquid propolis extract in semen diluents affects the cryopreservation efficiency of ram semen.</p><p><strong>Methods: </strong>Six Akkaraman breed rams were considered for semen study. Semens were combined with Tris+egg yolk extender containing and without (control) propolis at different concentrations (0.25%, 0.50%, 1%, 2%, and 4%). Semen was frozen using routine ram semen freezing procedures. After thawing, motility and kinematic parameters were analyzed by computer assisted semen analysis (CASA), and viability, acrosomal damage level and mitochondrial membrane potential were analyzed by flow-cytometer in all groups. Additionally, fatty acid levels in total semen were analyzed using gas chromatography (GC), and vitamin and cholesterol levels were analyzed using high-performance liquid chromatography (HPLC). In addition, oxidative stress, HOS test, and morphological analyzes were performed after freezing and thawing.</p><p><strong>Results: </strong>The 0.5% propolis group showed a significant increase in total and rapid motility, LIN, membrane integrity, and antioxidant levels compared to the control, and a significant decrease in malondialdehyde (MDA) and low mitochondrial membrane potential (LMMP) levels. Compared to the control, the group containing 4% propolis damaged spermatozoa and caused a significant decrease in total, progressive and rapid motility and high mitochondrial membrane potential (HMMP), Glutathione S-transferase (GST), and catalase (CAT) levels.</p><p><strong>Conclusion: </strong>We showed that adding 0.5% propolis to semen extenders to increase the freezability level of ram semen increases the survival of spermatozoa after freeze-thaw and ensures the success of freezing.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"250-260"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milking reactivity influences daily yield and electrical resistance of milk in Jaffarabadi buffaloes.","authors":"P Maurya, T K Patbandha, M D Odedra, V K Singh, S K Kalasava, P H Agravat","doi":"10.22099/IJVR.2024.49370.7245","DOIUrl":"https://doi.org/10.22099/IJVR.2024.49370.7245","url":null,"abstract":"<p><strong>Background: </strong>Buffalo reactivity during milking affects milking procedures, milk yield, and quality.</p><p><strong>Aims: </strong>This study evaluated the influence of milking reactivity on the yield, composition, somatic cell count, pH, and electrical resistance of milk in Jaffarabadi buffaloes.</p><p><strong>Methods: </strong>A 1-4 point scale, based on leg movement, was used to assess the milking reactivity of buffaloes (n=40). Based on the milking reactivity score, animals were classified into four groups: reactivity score-1 (RS1), reactivity score-2 (RS2), reactivity score-3 (RS3), and reactivity score-4 (RS4). The influence of milking reactivity on yield, composition, somatic cell count, pH, and electrical resistance of milk was observed.</p><p><strong>Results: </strong>Buffaloes with RS1 and RS2 produced significantly (P≤0.001) higher daily milk yield, 6% fat-corrected yield, solid-corrected yield, and energy-corrected yield than the RS3+4 group. Milking reactivity score did not influence milk fat, protein, lactose, ash, solid-not-fat, total solids content, and the fat: protein ratio. However, daily yield of milk fat (P<0.001), protein (P=0.001), lactose (P=0.001), ash (P=0.002), solid-not-fat (P=0.001), and total solids (P<0.001) were significantly higher in buffaloes in the RS1 and RS2 groups than in the RS3+4 group. Milk somatic cell count and somatic cell score were not influenced by milking reactivity score (P>0.05). Milk density and pH did not differ significantly (P>0.05) between groups. However, the electrical resistance of milk in the RS1 group was significantly (P<0.05) higher than in the RS2 and RS3+4 groups.</p><p><strong>Conclusion: </strong>Milking reactivity influences daily milk yield, milk component yield, and electrical resistance, but not milk composition in Jaffarabadi buffaloes.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"161-165"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of cell-free supernatant of lactic acid bacteria on spoilage bacteria of vacuum-packed sliced emulsion-type sausages.","authors":"S Tajbakhsh, M H Eskandari, S S Shekarforoush","doi":"10.22099/ijvr.2024.49361.7255","DOIUrl":"10.22099/ijvr.2024.49361.7255","url":null,"abstract":"<p><strong>Background: </strong>Spoilage is very common in vacuum-packed sliced emulsion-type sausages during refrigerated storage. <b>Aims:</b> This study aimed to investigate the inhibitory effects of cell-free supernatant (CFS) of lactic acid bacteria (LAB) on the spoilage bacteria isolated from vacuum-packed sliced emulsion-type sausages as biological preservatives.</p><p><strong>Methods: </strong>These products from various companies were examined to find the spoilage bacteria. A total of 43 LABs were screened for inhibitory activity against the spoilage bacteria. The MIC₉₀ of protective bacteria and the inhibitory effect of different components obtained from these bacteria was investigated.</p><p><strong>Results: </strong>Four LAB were confirmed as the predominant spoilage bacteria, including Enterococcus mundtii, <i>Latilactobacillus</i> sakei<i>,</i> Latilactobacillus curvatus, and Weissella viridescens. Six strains, including <i>Lactobacillus acidophilus</i> ATCC 4356, <i>Lactobacillus helveticus</i> PTCC 1332, <i>Lactiplantibacillus plantarum</i> CEC 17484, <i>Lactiplantibacillus plantarum</i> LL441, <i>Lacticaseibacillus rhamnosus</i> ATCC 53103, and <i>Pediococcus acidilactici</i> DSM 20284 were able to produce proteinaceous antimicrobial metabolites against the four spoilage agents, which were selected as protective bacteria. CFS of the protective bacteria inhibited four spoilage bacteria by more than 88%. The MIC₉₀ of all protective bacteria was less than 10 mg/ml against <i>E. mundtii</i> and <i>L. sakei</i>. After neutralizing acid and H₂O₂ of the CFS of <i>P.</i> <i>acidilactici</i>, it was still quite effective against <i>E. mundtii</i> and <i>L. sakei</i>. The sausage's pasteurization temperature did not affect the bacteria's active metabolites.</p><p><strong>Conclusion: </strong>The substances derived from these bacteria can be applied as biopreservatives in sliced sausage, even in the pre-pasteurization stage of these products.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"182-191"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic and apoptotic effects of zoledronic acid on D-17 canine osteosarcoma cell line.","authors":"G S Ekren Aşici, F Kiral","doi":"10.22099/ijvr.2024.47475.6877","DOIUrl":"10.22099/ijvr.2024.47475.6877","url":null,"abstract":"<p><strong>Background: </strong>Pets are exposed to a multitude of carcinogenic substances in the modern world. Consequently, high rates of cancer are observed, particularly in middle-aged cats and dogs. Although bisphosphonates have been incorporated into the treatment of human cancers, there are few animal-specific studies. As the number of cancer cases in animals continues to rise, it becomes increasingly important to evaluate anticancer drugs on a species-specific basis.</p><p><strong>Aims: </strong>The present study aimed to examine the impact of zoledronic acid (ZA) on apoptotic pathways in canine osteosarcoma (OSA) cell lines.</p><p><strong>Methods: </strong>The apoptotic effects of ZA administration on D-17 canine OSA cells were analysed by determining apoptotic DNA breaks, caspase-3, -8 and -9 levels by ELISA method and Bax/Bcl-2 ratio by qRT-PCR. The effect of ZA on the colony formation capacity of cells was evaluated by crystal violet staining method. The mineral binding capacity of ZA application in cells was investigated by Alizarin Red S staining technique. The change in alkaline phosphatase enzyme activity in OSA cells due to ZA treatment was determined using the colorimetric method. The antimetastatic effect was determined using the wound healing method, which evaluated the migration potential of cells.</p><p><strong>Results: </strong>While ZA application did not show a significant cytotoxic effect in the cells in the first 24 h, a decrease observed in the viability of the cells depending on the increasing dose and time. Low dose ZA (1, 5, 7.5, 10 µM) concentrations increased mineral content and alkaline phosphatase enzyme activity. A significant decrease was found in the expression levels of survivin, which determines the cell survival, depending on the dose and time.</p><p><strong>Conclusion: </strong>It is expected that our obtained data will contribute to the more effective treatment of the disease by creating different treatment options for clinicians in the light of increasing knowledge in cancer cell biology.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 3","pages":"192-201"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of antibiotic resistance, virulence genes, and biofilm formation of <i>Escherichia coli</i> isolated from sheep feces in Shiraz industrial slaughterhouse, South of Iran.","authors":"Z Naziri, A Hajihasani, A Derakhshandeh","doi":"10.22099/IJVR.2024.48215.7019","DOIUrl":"10.22099/IJVR.2024.48215.7019","url":null,"abstract":"<p><strong>Background: </strong>With the increase in human population, the consumption of livestock products such as sheep meat has also increased. Sheep are the reservoir and shedder of <i>Escherichia coli</i> that can be transmitted to humans. <b>Aims:</b> Characterization of fecal <i>E. coli</i> isolated from sheep in slaughterhouse.</p><p><strong>Methods: </strong>Stool specimens were collected from 30 apparently healthy sheep from different flocks in Shiraz industrial slaughterhouse. The resistance of <i>E. coli</i> isolates against 10 antibiotics was determined by disk diffusion method. The presence of three major extended spectrum beta-lactamase (ESBL) genes and five tetracycline resistance genes as well as seven virulence genes were investigated by polymerase chain reaction (PCR) technique. Using the microtiter plate method, the biofilm formation ability of <i>E. coli</i> isolates was investigated.</p><p><strong>Results: </strong>The highest frequency of resistance was to amoxicillin (100%) followed by tetracycline (25%). All <i>E. coli</i> isolates were susceptible to gentamicin and nitrofurantoin, and only one isolate was resistant to the tested third-generation cephalosporins. Multidrug resistance phenotype was observed in 16.7% of the isolates. <i>bla</i> _TEM (25%) was the most prevalent ESBL gene and <i>tetA</i> (62.5%) was the most prevalent tetracycline resistance gene in the isolates. <i>crl</i>, <i>csgA</i>, <i>fimH</i>, and <i>bcsA</i> genes were present in all isolates, and the prevalence of <i>papC</i> and <i>afa</i> genes was 95.8% and 83.3%, respectively. In total, 62.5% of the isolates were biofilm producers.</p><p><strong>Conclusion: </strong>According to the concept of One Health, the presence of virulent antibiotic-resistant biofilm producing strains of <i>E. coli</i> in sheep is a risk to public health.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"25-32"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and molecular characterization of fowl adenovirus from inclusion body hepatitis cases in Western India during 2019-2021.","authors":"V G Chavan, S P Awandkar, R C Kulkarni, S G Chavhan, R D Suryawanshi, R K Jadhav, M B Kulkarni, A A Agnihotri","doi":"10.22099/IJVR.2024.47035.6773","DOIUrl":"10.22099/IJVR.2024.47035.6773","url":null,"abstract":"<p><strong>Background: </strong>Inclusion body hepatitis (IBH) resulted in a substantial economic loss in Western India during 2019 to 2021.</p><p><strong>Aims: </strong>The study aimed to characterize fowl adenovirus (FAdV) from field outbreaks.</p><p><strong>Methods: </strong>The study was conducted on 290 liver samples from 66 poultry flocks. The samples were subjected to histopathology and molecular detection, followed by phylogenetic typing of the partial hexon gene of the virus.</p><p><strong>Results: </strong>Spiking mortality (14%) was recorded from day 21 to day 35 with peak mortality at the 28th day of age. The necropsy showed a pale and enlarged liver with hemorrhagic and yellowish necrotic foci, accumulation of straw-colored transudate in the pericardial sac which resulted in a flabby appearance of the heart, heart enlargement, and hemorrhages on the spleen, enlarged and congested kidneys. The virus inoculation resulted in stunting and poor feathering with hepatomegaly, hemorrhages and yellowish necrotic foci on the liver as well as greenish discoloration, and kidney swelling in SPF embryonated chicken eggs. Out of 29, 16 liver samples yielded 1219 bp amplicons specific to <i>hexon</i> gene fragments. The sequence and phylogenetic analysis identified 14 isolates as FAdV species E serotype 11 and two as species D serotype 8b. Conclusion: The results indicated that FAdV-8b and FAdV-11 strains are involved in disease outbreaks in western India.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"62-68"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the efficiency of estrus synchronization protocols combined with natural service and ultrasonography on ewe reproductive performance during non-breeding season.","authors":"P R Kumar","doi":"10.22099/IJVR.2024.48021.6981","DOIUrl":"10.22099/IJVR.2024.48021.6981","url":null,"abstract":"<p><strong>Background: </strong>Estrus synchronization is an important assisted reproductive technology to improve the reproductive performance in ewes. Various protocols have been used with variable success rates, however; literature regarding field applicable estrus synchronization is meagre.</p><p><strong>Aims: </strong>The present study was designed with the aim to evaluate the estrus synchronization protocols on reproductive performance in ewes using different hormones.</p><p><strong>Methods: </strong>Experimental ewes were divided randomly into three groups (n=15). Ewes of all groups received intravaginal sponge for 12 days. Subsequently, NP₄-GnRH and NP₄-eCG groups received 8 µg of buserelin acetate or 200 IU of eCG intramuscularly, respectively on day 12 whereas NP₄-Insulin group received insulin 0.2 IU/kg body weight subcutaneously for three consecutive days started on the day of sponge removal. Estrus detection commenced 24 h after sponge removal in NP₄-GnRH and NP₄-eCG groups and 24 h following last injection of insulin in NP₄-Insulin group. The ewes in estrus were separated and pen mated. The conception rate was determined by ultrasonography.</p><p><strong>Results: </strong>The estrus response and conception rates were 71.43, 92.86 and 53.85%, and 70.00, 84.61, and 71.43%, respectively in NP₄-GnRH, NP₄-eCG, and NP₄-Insulin groups. The lambing rates were the same as the conception rates. The single and multiple birth rates were 71.41, 36.36 and 60.0%, and 28.57, 63.64, and 40.0% whereas prolificacy was 128.57, 190.91, and 140.00%, respectively in NP₄-GnRH, NP₄-eCG, and NP₄-Insulin groups.</p><p><strong>Conclusion: </strong>In conclusion, the estrus synchronization protocol including intravaginal progesterone sponge and eCG was found to be more effective under field conditions.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"48-53"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the therapeutic potential of infrapatellar fat pad adipose-derived stem cells and their secretome for regenerating knee articular cartilage in a rat model of osteoarthritis.","authors":"S A Dalir, A Meimandi Parizi, N Tanideh, M Kian, F Nowzari, A Iraji, P Ghaemmagham, N Azarpira, Sh Zare","doi":"10.22099/IJVR.2024.47870.6959","DOIUrl":"10.22099/IJVR.2024.47870.6959","url":null,"abstract":"<p><strong>Background: </strong>Mesenchymal stem cell (MSC) therapy has ameliorative effects for treating knee osteoarthritis (KOA) disease. Moreover, there is a growing interest in using MSCs-derived secretome (Sec) containing trophic factors secreted by MSCs for KOA treatment. Recently, some studies have suggested that the combination of MSCs and Sec has the potential to treat the diseases.</p><p><strong>Aims: </strong>This study aimed to evaluate the ameliorative effects of combined administration of infrapatellar fat pad (IPFP)-derived MSCs, a type of adipose-derived stem cells (ASCs), for treating degenerated cartilage in a rat model of KOA.</p><p><strong>Methods: </strong>IPFP-ASCs were isolated from the IPFP of male rats. Sec was obtained from IPFP-ASCs in the fourth passage. Eight weeks after the induction of KOA by collagenase II, the rats were divided into 5 groups (n=5), including a control group with no treatment, and four experimental groups that received sodium hyaluronate (Hyalgan^®, Hya), ASCs, Sec, and IPFP-ASCs+Sec, respectively by an infrapatellar injection. To perform the pathological and radiological evaluations, the animals were sacrificed 8 weeks later.</p><p><strong>Results: </strong>Our findings indicated that combined administration of the IPFP-ASCs and Sec statistically (P<0.05) improved scores of medial tibial and femoral condyles and medial fabella osteophytes. Also, it statistically (P<0.05) enhances the cartilage surface, matrix, cell distribution and population viability, and subchondral bone indices. No statistical difference was observed between IPFP-ASCs+Sec and IPFP-ASCs.</p><p><strong>Conclusion: </strong>Administration of IPFP-ASCs+Sec has a therapeutic potential to treat KOA in rats. However, there is no difference in the combined administration of IPFP-ASCs and Sec with IPFP-ASCs alone.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 1","pages":"33-40"},"PeriodicalIF":0.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11327642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}