Iranian journal of veterinary research最新文献

{"title":"Effects of tamarind pulp powder supplementation on growth performance, haemato-immunological parameters, and immune-related gene expression in pacific white leg shrimp <i>(Litopenaeus vannamei)</i>.","authors":"B Lokesh, T Neeraja, Ch Lavanya, P Philominal","doi":"10.22099/ijvr.2025.51831.7714","DOIUrl":"10.22099/ijvr.2025.51831.7714","url":null,"abstract":"<p><strong>Background: </strong>Application of dietary phytobiotics against bacterial pathogens in shrimp farming is considered a promising alternative to antibiotics.</p><p><strong>Aims: </strong>The present study investigated the impact of tamarind pulp-powder (TPP) supplemented diets on growth, survival, immune responses, resistance to <i>Vibrio harveyi</i>, and immune gene expression in <i>Litopenaeus vannamei</i>.</p><p><strong>Methods: </strong>A 49-day feeding trial was conducted using <i>L.</i> <i>vannamei</i> (mean weight 5.60±0.45 g) fed with TPP-supplemented diets containing 0 (T0), 1 (T1), 5 (T2), and 10 (T3) g/kg diets at 3% body weight. Growth performance viz., net weight gain (NWG) and feed conversion ratio (FCR) were recorded on weekly basis and immune responses viz., serum protein (SP), Prophenoloxidase (PPO), and lysozyme (LZ) activities were analyzed at the end. A challenged test conducted for 96 h with <i>V. harveyi</i> (1×10⁵ CFU/ml) to assess mortality. After the challenge test, enzymatic activities and immune genes viz., toll-like receptor and lysozyme were analyzed.</p><p><strong>Results: </strong>Shrimp fed with the T2 diet (5 g/kg TPP) showed significantly (P< 0.05) higher NWG, improved FCR, and survival compared to other groups. Total hemocyte count, SP, PPO, and LZ activities were significantly higher in T2 shrimp. Reduced levels of enzymatic activities and upregulated expression of immune-related genes were also observed in T2. During the challenge, T2-fed shrimp achieved the highest survival rate (86%), confirming enhanced resistance to <i>V. harveyi</i>.</p><p><strong>Conclusion: </strong>A dietary inclusion level of 5 g/kg TPP was found optimal for improving growth, immunity, and resistance against vibriosis in <i>L. vannamei</i>, highlighting its potential as a phytobiotic alternative to antibiotics in shrimp aquaculture.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 4","pages":"360-369"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and evaluation of homologous IgG on canine parvovirus.","authors":"M Bakhshesh, M H Motedayen, S Jamshidi, N Azadi, N Zarifian, M H Rabiee, N Ghahari","doi":"10.22099/ijvr.2025.52828.7901","DOIUrl":"10.22099/ijvr.2025.52828.7901","url":null,"abstract":"<p><strong>Background: </strong>Canine parvovirus, an important and highly pathogenic agent for puppies, is distributed worldwide. Despite the various vaccines being available, it is not easily prevented. Immunoglobulin therapy has been attempted as a supportive approach to cure affected puppies.</p><p><strong>Aims: </strong>In order to achieve a homologous immunoglobulin G (IgG) for the treatment of the fatal parvovirus, the purification of anti-parvovirus IgG from immunized dogs was assayed.</p><p><strong>Methods: </strong>IgG purification was conducted on the obtained immune sera using two-stage dialysis, in which various concentrations of ammonium sulfate with several incubations and centrifugations were applied in multiple steps.</p><p><strong>Results: </strong>Using SDS-PAGE electrophoresis, the correct band sizes of the purified IgGs were observed and its concentration was assessed to be >22 mg/ml. Also, using quantitative ELISA, the specific anti-parvovirus IgG titer of the purified dog IgG was calculated to be considerably above the protective threshold titer. To do so, 13 parvoviral diarrheic puppies were randomly categorized into 3 treatment groups for 5 consecutive days. Administration of 4, 8 and 16 mg/kg bw/d of purified IgG to these puppies significantly improved all clinical symptoms including diarrhea, dehydration, appetite, vomiting and white blood cell (WBC) in all affected puppies in dose-dependent manner, comparing with those in the placebo-controlled group (n=8) that experienced 2 deaths, too.</p><p><strong>Conclusion: </strong>This homologous anti-parvovirus IgG, which was purified through a novel, simple and inexpensive technology is expected to pass further clinical trials. Also, this IgG has the potential as a commercial product to reduce the burden of parvovirus infection in puppies.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 4","pages":"333-340"},"PeriodicalIF":0.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatitis-hydropericardium syndrome in native chickens: A clinicopathological study.","authors":"S Alidadi, A Ghaniei, E Cheshmehsangi","doi":"10.22099/ijvr.2025.50868.7529","DOIUrl":"10.22099/ijvr.2025.50868.7529","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis-Hydropericardium syndrome (HHS), a significant disease in poultry, is caused by fowl adenoviruses (FAdV). While common in commercial flocks, HHS has not been previously documented in native chicken breeds in Iran.</p><p><strong>Case description: </strong>This case report describes HHS in a small backyard flock of native hens in Mashhad, Iran. The affected birds, three 6-month-old hens, exhibited lethargy for one day prior to death.</p><p><strong>Findings/treatment and outcome: </strong>Post-mortem examination revealed hydronephrosis, splenomegaly, hydropericardium, and hepatic necrosis. Histopathological examination showed significant lesions in the liver, heart, kidney, and spleen. The liver displayed congestion, hemorrhage, hepatocellular degeneration, necrosis, lymphocytic hepatitis, and large basophilic intranuclear inclusion bodies in the affected hepatocytes, confirmed by Lendrum's phloxine-tartrazine method. Cardiac lesions included pericarditis, myocarditis, edema, and myofiber degeneration and necrosis. The spleen exhibited congestion and hemorrhage, while renal tissues showed tubular epithelial cell degeneration and necrosis with inflammation. PCR assay on the liver samples confirmed FAdV serotype 4.</p><p><strong>Conclusion: </strong>These findings show HHS and a FAdV serotype 4 etiology, highlighting the emerging threat of adenoviral diseases to noncommercial poultry settings in Iran.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 2","pages":"189-193"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12640158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual histopathological features of a uterine leiomyosarcoma in a dog.","authors":"T R Akhila, T N Ganesh, A Azimah, M K Kok, A Salleh","doi":"10.22099/ijvr.2025.50585.7473","DOIUrl":"10.22099/ijvr.2025.50585.7473","url":null,"abstract":"<p><strong>Background: </strong>Uterine leiomyosarcoma is a malignant neoplasm arising from leiomyocytes in the uterus.</p><p><strong>Case description: </strong>A 12-year-old mixed-breed intact female dog was presented with vaginal bleeding for the past 2 weeks. Abdominal ultrasonography revealed a mixed echogenic mass in the uterus, and right lateral abdominal radiography showed a large mid-abdominal mass. Exploratory celiotomy was performed, revealing a solid mass in the entire uterine body and both uterine horns. Ovariohysterectomy was performed and the uterine mass sample was microscopically examined.</p><p><strong>Findings/treatment and outcome: </strong>The mass was composed of a dense proliferation of neoplastic cells that display two morphologic features; spindloid and epithelioid appearances. Additionally, chondroid-like matrix was noted. Both components were negative for cytokeratin AE1/AE3, and strongly positive for vimentin. The spindloid component was strongly positive for α-SMA, while the epithelioid-like component was moderately positive for alpha-smooth muscle actin. These findings are suggestive of a leiomyosarcoma. Although the recovery immediately following surgery was smooth and without complications, the dog's condition worsened over the following month, ultimately leading to euthanasia.</p><p><strong>Conclusion: </strong>This report describes the histopathologic features and clinical outcome of an unusual variant of uterine leiomyosarcoma in a dog.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"88-93"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423996/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antibiogram profiling of enterotoxigenic methicillin-resistant <i>Staphylococcus aureus</i> at bovine-human interface.","authors":"M Ijaz, M U Javed, A Ahmed, H Rasheed, M J Sabir, A A Jabir, M Batool","doi":"10.22099/ijvr.2024.50719.7502","DOIUrl":"10.22099/ijvr.2024.50719.7502","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i>, a ubiquitous pathogen due to its key involvement in dairy animal mastitis, is the leading cause of food-borne diseases in humans by producing various enterotoxins. <b>Aims:</b> The present study reported the prevalence of significantly increased enterotoxigenic MRSA pathogens among bovines and dairy occupational workers, along with antibiotic-resistant patterns, using the <i>in-vitro</i> technique.</p><p><strong>Methods: </strong>A total of 384 bovine (n=192 cattle, n=192 buffalo) milk samples and 100 human nasal or skin swab samples were collected to find out the prevalence of <i>S. aureus</i>, MRSA, <i>spa</i>, and enterotoxin genes (<i>seb</i>, <i>sec</i>) by PCR. Also, a phylogenetic analysis was conducted to compare and analyze the prevalent enterotoxin <i>seb</i> gene sequences from bovines, workers, and other sources.</p><p><strong>Results: </strong>The present study revealed that out of 484 total samples, 49.79% of isolates were positive for <i>S. aureus</i> while 29.46% and 66.80% of <i>S. aureus</i> isolates were positive for MRSA, and <i>spa</i> genes among bovine and human samples collectively. The prevalence of enterotoxigenic <i>S. aureus</i> was found to be 16.18% in bovine and human staphylococcal isolates. Additionally, the enterotoxigenic strains exhibited resistance to commonly used antibiotics.</p><p><strong>Conclusion: </strong>The present study shows that enterotoxigenic MRSA is prevalent in bovines and dairy occupational workers of study districts, Pakistan, and study isolates revealed a varying level of resistance to different antibiotics. The various virulence factors along with the antibiotic resistance makes MRSA a potential threat at animal-human interface, highlighting the need for further research.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"8-16"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12424000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular typing and seroepidemiology of pathogenic <i>Leptospira</i> spp. of domestic animals in Gilan province, northern Iran during 2021-2023.","authors":"B Talebi Darestani, S T Shafighi, G Abdollahpour, L Asadpour, N Ranji","doi":"10.22099/ijvr.2025.48812.7141","DOIUrl":"10.22099/ijvr.2025.48812.7141","url":null,"abstract":"<p><strong>Background: </strong><i>Leptospira</i>, a pathogenic bacterial organism, causes human leptospirosis when it infects mammals. There are different serovars of <i>Leptospira</i>, each of which is adapted to a specific animal host. Adaptation occurs dynamically in different hosts and changes the distribution and clinical manifestations of serovars. Variable-number tandem repeats (VNTRs) have been used to identify <i>Leptospira</i> <i>interrogans</i> strains.</p><p><strong>Aims: </strong>The purpose of this study was to conduct seroepidemiology and molecular typing of pathogenic <i>Leptospira</i> isolated from domestic animals in Gilan province.</p><p><strong>Methods: </strong>A total number of 250 urine and blood samples were collected from domestic animals (horse, bovine, and sheep). Urine samples were cultured in EMJH medium. Using the microscopic agglutination test (MAT) and the Multilocus VNTR Analysis (MLVA) methods, unknown samples were analyzed to determine <i>Leptospira</i> serovars.</p><p><strong>Results: </strong>Based on our data, <i>Leptospira</i> Autumnalis was mainly found in horses and dominated at 50%, followed by <i>Leptospira</i> Canicola at 29.16%. MAT results showed that among 87 animals with the seropositive, the horses showed the highest level of contamination at 40%, and this difference was not significant (P=0.152).</p><p><strong>Conclusion: </strong>According to our data, <i>Leptospira</i> Autumnalis was mainly found in horses, and the bacteria were shed in urine, suggesting that horses could transmit leptospirosis. The results of this study confirm that MLVA can replace time-consuming methods such as MAT because it is a simple and fast technique.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"41-50"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat shock protein D1 is up-regulated in various types of canine mammary tumors.","authors":"G Kaur, B V S Kumar, K Gupta","doi":"10.22099/ijvr.2024.50204.7404","DOIUrl":"10.22099/ijvr.2024.50204.7404","url":null,"abstract":"<p><strong>Background: </strong>Mammary tumors are the most common tumors in female dogs. An early diagnosis makes the treatment easier.</p><p><strong>Aims: </strong>The present study aimed to assess heat shock protein D1 (HSPD1) expression in canine mammary tumors.</p><p><strong>Methods: </strong>Canine mammary tumor (CMT) samples were collected from clinics after surgery. Expression of <i>HSPD1</i> transcript in CMT and apparently healthy mammary tissues was analyzed by SYBR green based real-time PCR (qRT-PCR). Further, gene encoding the immuno-dominant region of HSPD1 was cloned using the expression vector pPROEX-HTa and expressed in a prokaryotic system and recombinant HSPD1 (rHSPD1) was purified by affinity chromatography. Hyperimmune serum was raised against rHSPD1 in mice, and immunohistochemistry was standardized to assess the expression of this protein in various histotypes of canine mammary tumors.</p><p><strong>Results: </strong>An elevated <i>HSPD1</i> mRNA expression (5.973 ± 0.862 folds) was observed in canine mammary tumors. Upon purification, a 60 kDa recombinant protein was obtained and confirmed by Western blotting. In 83.3% of healthy mammary tissues, a mild/feeble HSPD1 expression was observed whereas, a strong expression of HSPD1 was seen in 80% CMT samples.</p><p><strong>Conclusion: </strong>The findings suggested that HSPD1 could be used as a molecular marker for canine mammary tumors.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"368-375"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutrient content, <i>in-vitro</i> digestibility, and starch and protein molecular appearance of intact and ammoniated steamed-flaked and/or steamed-infrared heated-flaked barley grain.","authors":"A Honarmand, A R Vakili, M Danesh Mesgaran, A M Tahmasebi","doi":"10.22099/ijvr.2024.49168.7210","DOIUrl":"10.22099/ijvr.2024.49168.7210","url":null,"abstract":"<p><strong>Background: </strong>The impact of different physical and/or chemical treatments in cereal grains on starch morphology and ruminal digestibility has been evaluated.</p><p><strong>Aims: </strong>The effect of chemical and/or physical treatments on starch and protein molecular appearance and the <i>ex-vivo</i> digestibility of barley grain was studied.</p><p><strong>Methods: </strong>Treatments were: steam-flaked barley grain (SFB), SFB treated with ammonium bicarbonate (A), urea (U), and malic acid (M) (SFB_AUM), SFB treated with A, U, and lactic acid (L) (SFB_AUL), steam-infrared heated-flaked barley grain (SIFB), SIFB treated with A, U, and M (SIFB_AUM), and SIFB treated with A, U, and L (SIFB_AUL). Chemicals including A, U, M, and L were used as 56, 8, 10, and 10 g/kg dry matter (DM), respectively. Chemical composition and molecular morphology were determined using scanning electron microscopy and Fourier-transform infrared spectroscopy (FTIR). In situ mobile bag technique and <i>in vitro</i> batch culture procedure were used to estimate ruminal and post-ruminal digestibility.</p><p><strong>Results: </strong>Crude protein (CP) and starch concentrations in SFB_AUL were higher than the others (P<0.05). Starch granule morphology and protein structure were altered in the chemically treated samples. The potentially digestible fraction of DM was the highest in the SFB_AUM (P<0.05). Ruminal disappearance of DM, CP, and starch was improved in SFB_AUL and SIFB_AUL compared with other groups (P<0.05). The highest post-ruminal digestibility of starch and CP was observed in SIFB_AUL and SIFB (P<0.05).</p><p><strong>Conclusion: </strong>Present results indicate that chemical processing with L and applied steam-infrared heated-flaked in barley grain may improve <i>in vitro</i> digestibility of starch and CP and increase granule sizes.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"376-387"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Respiratory and intestinal zoonotic cryptosporidiosis in symptomatic domestic pigeons (<i>Columba livia domestica</i>) in Tabriz, Iran.","authors":"M Khordadmehr, A Sazmand, A Oryan, P Almasi, V Ranjbar, A Hemphill, D Otranto","doi":"10.22099/ijvr.2024.49762.7335","DOIUrl":"10.22099/ijvr.2024.49762.7335","url":null,"abstract":"<p><strong>Background: </strong>Domestic pigeons (Columba livia domestica<i>)</i> are the oldest domesticated birds worldwide, harboring many zoonotic parasites and posing potential public health threats.</p><p><strong>Aims: </strong>To investigate cryptosporidiosis in domestic pigeons in Tabriz, Iran, 100 privately owned pigeons presenting weight loss and diarrhea were tested for Cryptosporidium spp. through parasitological, histopathological, and molecular tests.</p><p><strong>Methods: </strong>Modified Ziehl-Neelsen-stained fecal smears and histological sections of the trachea and small intestine were examined microscopically. Genomic DNA of fecal and tracheal specimens was examined by nested conventional PCR targeting <i>18S rDNA</i>, followed by Sanger sequencing of histopathology-confirmed samples and phylogenetic analyses.</p><p><strong>Results: </strong>All pigeons were positive at PCR in their feces and trachea. Oocysts similar to the size of Cryptosporidium species were observed in stained fecal smears of 62% of pigeons. At the histopathological examination, Cryptosporidium<i>-</i>organisms were observed on the apical epithelial surfaces of the small intestine in 84% and trachea in 78% of pigeons. In 23 pigeons, simultaneous tracheal and intestinal cryptosporidiosis was determined. The lesions in affected tracheas and small intestines included hyperemia, villous atrophy and fusion, dilatation of intestinal crypts, irregular epithelial hyperplasia, and sloughing. Diffused mixed inflammatory cell infiltration in the lamina propria was observed, with dominant lymphocytes, plasma cells, and lower numbers of heterophils. Consensus sequences of detected parasites revealed infection with Cryptosporidium parvum and Cryptosporidium meleagridis.</p><p><strong>Conclusion: </strong>Considering the high frequency of cryptosporidiosis reported here in symptomatic birds and that both identified Cryptosporidium species are zoonotic parasites, findings claim a public health risk assessment of this species of animals.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 4","pages":"326-332"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>FASN</i> gene polymorphism on milk production traits in Bangladeshi cattle: Insights from mutant protein function.","authors":"M A Alim, M M K Hossain, N Jahan, R Rubaya, A A Bhuyan, J Alam, I Ahammad, M S R Siddiki, M Moniruzzaman","doi":"10.22099/ijvr.2025.49855.7351","DOIUrl":"10.22099/ijvr.2025.49855.7351","url":null,"abstract":"<p><strong>Background: </strong>To meet up the demand gap of milk in Bangladesh, short-term, midterm, and long-term goal have been set up by the government through crossing with Bangladeshi local cattle and high-producing foreign cattle like Friesian, Jersey, Sahiwal, etc.</p><p><strong>Aims: </strong>The purpose of this study was to identify the single nucleotide polymorphisms (SNPs) in the <i>FASN</i> gene, to check the structural and functional impact of mutant proteins on milk production traits that are significantly associated.</p><p><strong>Methods: </strong>Four SNPs were identified in exons 26, 36, 38, and 41 of the <i>FASN</i> gene using pooled DNA sequencing, but only one SNP g.17924 A>G was a non-synonymous that changed the amino acid threonine to alanine in the FASN protein and the other three SNPs were silent mutations. Structural and functional prediction analysis were done with a series of techniques to detect remote protein homology and predict structures, structural integrity, structure quality, protein stability, protein motion, flexibility, and stability impact, conservation profile and finally molecular dynamics simulations for wild-type and mutant protein expression differences.</p><p><strong>Results: </strong>The non-synonymous g.17924 A>G mutation showed a clear difference between wild and mutant proteins, indicating the impact on the observed phenotype. Then, SNP g.17924 A>G was genotyped in 100 milking cows aiming to check the association effects. SNP g.17924 A>G was found to have significant allele substitution effects on milk yield traits.</p><p><strong>Conclusion: </strong>Our results suggest that the identified polymorphism affects milk yield traits in the studied population and could be used as genetic marker for cattle selection processes aiming to increase productivity.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"26 1","pages":"68-77"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12424002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}