Effects of propolis supplementation during cryopreservation of ram semen.

IF 0.8 4区 农林科学 Q3 VETERINARY SCIENCES
I H Güngör, Ş Özer Kaya, S Dayan Cinkara, A Çakır Cihangiroğlu, E Kaya, T C Acısu, F Erdem Erişir, Ö Yılmaz, S Yılmaz, S Gür, M Sönmez, G Türk
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引用次数: 0

Abstract

Background: Cryopreservation of ram semen is a very challenging process. Loss of motility during freezing does not allow artificial insemination of rams.

Aims: This study aimed to determine whether the inclusion of liquid propolis extract in semen diluents affects the cryopreservation efficiency of ram semen.

Methods: Six Akkaraman breed rams were considered for semen study. Semens were combined with Tris+egg yolk extender containing and without (control) propolis at different concentrations (0.25%, 0.50%, 1%, 2%, and 4%). Semen was frozen using routine ram semen freezing procedures. After thawing, motility and kinematic parameters were analyzed by computer assisted semen analysis (CASA), and viability, acrosomal damage level and mitochondrial membrane potential were analyzed by flow-cytometer in all groups. Additionally, fatty acid levels in total semen were analyzed using gas chromatography (GC), and vitamin and cholesterol levels were analyzed using high-performance liquid chromatography (HPLC). In addition, oxidative stress, HOS test, and morphological analyzes were performed after freezing and thawing.

Results: The 0.5% propolis group showed a significant increase in total and rapid motility, LIN, membrane integrity, and antioxidant levels compared to the control, and a significant decrease in malondialdehyde (MDA) and low mitochondrial membrane potential (LMMP) levels. Compared to the control, the group containing 4% propolis damaged spermatozoa and caused a significant decrease in total, progressive and rapid motility and high mitochondrial membrane potential (HMMP), Glutathione S-transferase (GST), and catalase (CAT) levels.

Conclusion: We showed that adding 0.5% propolis to semen extenders to increase the freezability level of ram semen increases the survival of spermatozoa after freeze-thaw and ensures the success of freezing.

添加蜂胶对公羊精液冷冻保存的影响。
背景:公羊精液的低温保存是一个非常具有挑战性的过程。在冷冻期间失去运动能力的公羊不允许人工授精。目的:研究蜂胶液体提取物在精液稀释液中的掺入对公羊精液冷冻保存效果的影响。方法:选取6只阿卡拉曼公羊进行精液研究。将精液与不同浓度(0.25%、0.50%、1%、2%和4%)含和不含(对照)蜂胶的Tris+蛋黄膨化剂组合。使用常规的公羊精液冷冻程序冷冻精液。解冻后,用计算机辅助精液分析(CASA)分析各组动物的运动和运动学参数,用流式细胞仪分析各组动物的生存能力、顶体损伤水平和线粒体膜电位。此外,用气相色谱(GC)分析了总精液中的脂肪酸水平,用高效液相色谱(HPLC)分析了维生素和胆固醇水平。冻融后进行氧化应激、HOS试验和形态学分析。结果:与对照组相比,0.5%蜂胶组的总和快速运动性、LIN、膜完整性和抗氧化水平显著增加,丙二醛(MDA)和低线粒体膜电位(LMMP)水平显著降低。与对照组相比,添加4%蜂胶组精子损伤显著,总运动、进行性和快速运动、高线粒体膜电位(HMMP)、谷胱甘肽s转移酶(GST)和过氧化氢酶(CAT)水平显著降低。结论:在精液填充剂中添加0.5%蜂胶,提高公羊精液的冷冻性水平,可提高精子冻融后的存活率,确保冷冻成功。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.70
自引率
8.30%
发文量
10
期刊介绍: The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
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