On-farm epidemiology and phylogenetic evaluation of methicillin and beta-lactam-resistant Staphylococcus aureus isolated from dairy cattle and buffaloes with endometritis.
{"title":"On-farm epidemiology and phylogenetic evaluation of methicillin and beta-lactam-resistant <i>Staphylococcus aureus</i> isolated from dairy cattle and buffaloes with endometritis.","authors":"P Bakht, M Ijaz, M Z Iqbal, H B Aslam, A Rehman","doi":"10.22099/IJVR.2024.48563.7095","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a potential emerging and prevailing multidrug-resistant (MDR) pathogen involved in bovine endometritis.</p><p><strong>Aims: </strong>Present research evaluated the prevalence and molecular characterization of methicillin-resistant <i>S. aureus</i> (MRSA) and beta-lactam resistant <i>S. aureus</i> (BRSA) and also analyzed the associated risk factors with endometritis along with antibiotic resistance patterns.</p><p><strong>Methods: </strong>A total of 384 uterine and vaginal fluid samples were collected from the adult dairy cattle and buffaloes showing the clinical signs of endometritis including foul-smelling vaginal discharge, fever, enlarged and thickened uterine horns on rectal palpation, and confirmation by ultrasonography findings. The collected samples were subjected to standard microbiological methods for the detection of <i>S. aureus</i>. The confirmed isolates were further subjected to the Kirby-Bauer disc diffusion test and the detection of the <i>mecA</i> and <i>blaZ</i> genes for the confirmation of MRSA and BRSA.</p><p><strong>Results: </strong>Study found an overall prevalence of 17.96% for <i>S. aureus</i> from bovine endometritis cases. Among <i>S. aureus</i> isolates, 50.72% and 37.68% isolates were confirmed MRSA while BRSA was found as 36.23% and 18.84%, based on phenotypic and genotypic methods, respectively. Phylogenetic analysis indicated the possibility of pathogen transmission within and between livestock animals. Risk factor analysis showed that the breed of animal, visible discharge from vagina, lactation number, insemination procedure, and calving place showed significant (P<0.05) association with <i>S. aureus</i>-associated endometritis. Antimicrobial susceptibility testing of study isolates showed the resistance to various commonly used antibiotics.</p><p><strong>Conclusion: </strong>The study concluded that <i>S. aureus</i> is found in 17.96% of bovines affected with endometritis and require further intensive research to elucidate the farm economic losses.</p>","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"25 2","pages":"98-106"},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608531/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2024.48563.7095","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Staphylococcus aureus is a potential emerging and prevailing multidrug-resistant (MDR) pathogen involved in bovine endometritis.
Aims: Present research evaluated the prevalence and molecular characterization of methicillin-resistant S. aureus (MRSA) and beta-lactam resistant S. aureus (BRSA) and also analyzed the associated risk factors with endometritis along with antibiotic resistance patterns.
Methods: A total of 384 uterine and vaginal fluid samples were collected from the adult dairy cattle and buffaloes showing the clinical signs of endometritis including foul-smelling vaginal discharge, fever, enlarged and thickened uterine horns on rectal palpation, and confirmation by ultrasonography findings. The collected samples were subjected to standard microbiological methods for the detection of S. aureus. The confirmed isolates were further subjected to the Kirby-Bauer disc diffusion test and the detection of the mecA and blaZ genes for the confirmation of MRSA and BRSA.
Results: Study found an overall prevalence of 17.96% for S. aureus from bovine endometritis cases. Among S. aureus isolates, 50.72% and 37.68% isolates were confirmed MRSA while BRSA was found as 36.23% and 18.84%, based on phenotypic and genotypic methods, respectively. Phylogenetic analysis indicated the possibility of pathogen transmission within and between livestock animals. Risk factor analysis showed that the breed of animal, visible discharge from vagina, lactation number, insemination procedure, and calving place showed significant (P<0.05) association with S. aureus-associated endometritis. Antimicrobial susceptibility testing of study isolates showed the resistance to various commonly used antibiotics.
Conclusion: The study concluded that S. aureus is found in 17.96% of bovines affected with endometritis and require further intensive research to elucidate the farm economic losses.
期刊介绍：
The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
