Genetic and phylogenetic evaluations of Schistosoma turkestanicum isolated from goats in Western Iran.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2024-01-01 DOI:10.22099/ijvr.2024.49135.7203

H Nayebzadeh, N Nazifi, H R Shokrani, Sh Yavari

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引用次数: 0

Abstract

Background: Schistosomiasis, a zoonotic parasitic infection, poses significant challenges to the animal husbandry industry, leading to substantial economic losses. Despite its impact, there is limited data on the genotypes of Schistosoma (=Orientobilharzia) turkestanicum in Iran.

Aims: The present study aimed to evaluate the phylogenetic relationships of Schistosoma turkestanicum isolated from goats by analyzing the mitochondrial cytochrome C oxidase subunit 1 (Cox1) gene sequence in Lorestan province, located in Western Iran.

Methods: DNA extraction was performed on 20 male parasitic worms, and the mitochondrial Cox1 gene was amplified using the polymerase chain reaction (PCR) process, and sent for sequencing after purification by ethanol alcohol. The sequences were trimmed using CLC Main Workbench software. Phylogenetic analysis was conducted using the Neighbor-Joining method with 1000 bootstrap replicates in MEGA6 software to assess evolutionary relationships between the Cox1 gene sequence obtained in this study (GenBank accession No. PP627151) and various S. turkestanicum sequences obtained from the National Center for Biotechnology Information (NCBI). Additionally, this software was also used to plot the genetic distance matrix (nucleotide differences and similarities).

Results: Phylogenetic analysis confirmed that the parasite isolated in this study was S. turkestanicum.

Conclusion: The findings indicate that the S. turkestanicum lineage identified in this study is closely related to those found in Mazandaran province, Iran, as well as to African Schistosoma species.

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伊朗西部山羊土耳其斯坦血吸虫分离株的遗传和系统发育评价。

背景：血吸虫病是一种人畜共患寄生虫感染，对畜牧业构成重大挑战，造成重大经济损失。尽管它有影响，但伊朗土耳其斯坦血吸虫基因型的数据有限。目的：通过分析伊朗西部Lorestan省山羊分离的土耳其斯坦血吸虫线粒体细胞色素C氧化酶亚基1 （Cox1）基因序列，探讨土耳其斯坦血吸虫的系统发育关系。方法：对20只雄性寄生虫进行DNA提取，采用聚合酶链反应（PCR）法扩增线粒体Cox1基因，经乙醇纯化后送测序。使用CLC Main Workbench软件对序列进行裁剪。采用Neighbor-Joining法，在MEGA6软件中进行1000个bootstrap重复的系统发育分析，以评估本研究获得的Cox1基因序列(GenBank登录号：GenBank；PP627151)和从国家生物技术信息中心（NCBI）获得的各种土耳其链球菌序列。此外，该软件还用于绘制遗传距离矩阵（核苷酸差异和相似性）。结果：系统发育分析证实本研究分离到的寄生虫为土耳其链球菌（S. turkestanicum）。结论：本研究鉴定的土耳其斯坦血吸虫谱系与伊朗Mazandaran省发现的血吸虫谱系以及非洲血吸虫种具有密切的亲缘关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.