International journal of systematic and evolutionary microbiology最新文献

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 76, part 7 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.007280","DOIUrl":"https://doi.org/10.1099/ijsem.0.007280","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149297465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated genomic analyses support the reclassification of <i>Arthrobacter vasquezii</i> Valenzuela-Ibaceta <i>et al</i>. 2023 as a later heterotypic synonym of <i>Arthrobacter parietis</i> Heyrman <i>et al.</i> 2005.","authors":"Khaoula Bouznada, Rafika Saker, Manel Zahida Bellouti, Guendouz Dif, Atika Meklat","doi":"10.1099/ijsem.0.007329","DOIUrl":"https://doi.org/10.1099/ijsem.0.007329","url":null,"abstract":"<p><p>The genus <i>Arthrobacter</i> Conn and Dimmick 1947 (Approved Lists 1980) encompasses a large and taxonomically complex assemblage of species within the family <i>Micrococcaceae</i>, many of which were described before whole-genome sequencing became a routine practice of prokaryotic taxonomy. During a comparison of genome sequences of <i>Arthrobacter</i> type strains, the type strain of <i>Arthrobacter vasquezii</i> Valenzuela-Ibaceta <i>et al.</i> 2023 (EH-1B-1^T), isolated from Antarctic soil, was found to be genomically indistinguishable from the type strain of <i>Arthrobacter parietis</i> Heyrman <i>et al.</i> 2005 (LMG 22281^T), isolated from a deteriorated mural painting. Pairwise comparison of the two type-strain genome sequences yielded a digital DNA-DNA hybridization (dDDH) value of 90.1% (95% CI 87.8-92.0%), an average nucleotide identity (ANI) of 98.96% and an average amino acid identity (AAI) of 99.15%, all well above the accepted thresholds for species delineation (dDDH ≥70%; ANI/AAI ≥95-96%). This congruence is further supported by all 16S rRNA gene sequence-based tree topologies and core genome phylogenomics and is consistent with comparative carbohydrate-active enzyme profiling and phenotypic analyses. As <i>A. parietis</i> Heyrman <i>et al.</i> 2005 has nomenclatural priority, we propose that <i>A. vasquezii</i> Valenzuela-Ibaceta <i>et al.</i> 2023 be reclassified as its later heterotypic synonym.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149297507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Agromyces molybdatiresistens</i> sp. nov. and <i>Agromyces selenitireducens</i> sp. nov., two molybdate/selenite co-resistant and reducing bacteria isolated from riverbed silt.","authors":"Xiuting Zhang, Kaiyue Wang, Yuanshuo Qiu, Zihan Yu, Xinping Liu, Zhilu Feng, Zuoqing Yuan, Aijv Liu, Rui Zhang, Hongliang Liu","doi":"10.1099/ijsem.0.007320","DOIUrl":"https://doi.org/10.1099/ijsem.0.007320","url":null,"abstract":"<p><p>Two actinobacterial strains with resistance to selenite and molybdate, designated as ZXT2-3^T and ZXT2-6^T, were isolated from the river sediment sample of Jinshui River in Xingtai City, Hebei Province, China. Based on 16S rRNA gene sequence analysis, strain ZXT2-3^T showed the highest sequence similarity to <i>Agromyces binzhouensis</i> CGMCC 4.7180^T (98.5%), and strain ZXT2-6^T was most closely related to <i>Agromyces arachidis</i> JCM 19251^T (99.1%), <i>Agromyces indicus</i> CCTCC AB 2011122^T (98.8%), <i>Agromyces aurantiacus</i> DSM 14598^T (98.6%), <i>Agromyces tropicus</i> JCM 15672^T (98.4%), <i>Agromyces mediolanus</i> JCM 3346^T (98.2%) and <i>Agromyces soli</i> JCM 16247^T (98.0%). The 16S rRNA gene sequence similarity between strains ZXT2-3^T and ZXT2-6^T was 98.4%. Phylogenetic analysis based on 16S rRNA gene sequences and whole-genome sequences indicated that strains ZXT2-3^T and ZXT2-6^T belong to the genus <i>Agromyces</i>. The genome sizes of strains ZXT2-3^T and ZXT2-6^T were 3.9 and 4.0 Mb, with DNA G+C contents of 72 and 71.5 %, respectively. The average nucleotide identity and digital DNA-DNA hybridization values with the closest relatives were below species delineation thresholds. The major fatty acids detected in strain ZXT2-3^T included anteiso-C_{15 : 0}, iso-C_{16 : 0}, anteiso-C_{17 : 0}, C_{16 : 0} and iso-C_{15 : 0}. For strain ZXT2-6^T, the major cellular fatty acids were identified as anteiso-C_{15 : 0}, anteiso-C_{15 : 1}A, iso-C_{16 : 0} and anteiso-C_{17 : 0}. The major respiratory quinones of strains ZXT2-3^T and ZXT2-6^T were MK-11 and MK-12. The polar lipid profiles of both strains contained diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids. Both isolates could reduce Se(IV) to produce selenium-containing particles. Strain ZXT2-3^T was also capable of reducing Mo(VI) to molybdenum blue. According to the phenotypic, genotypic and phylogenetic data, strains ZXT2-3^T and ZXT2-6^T represent two novel species of the genus <i>Agromyces</i>, for which <i>Agromyces molybdatiresistens</i> sp. nov. and <i>Agromyces selenitireducens</i> sp. nov. are proposed, respectively. The type strains are ZXT2-3^T (=GDMCC 1.4963^T=JCM 37791^T=MCCC 1K10748^T) and ZXT2-6^T (=GDMCC 1.4965^T=JCM 37792^T=MCCC 1K10749^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149238352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Christiangramia pacifica</i> sp. nov. and <i>Christiangramia profundi</i> sp. nov.<i>,</i> two novel species isolated from deep-sea environments of the Pacific Ocean.","authors":"Yangjie Lin, Kai Zhang, Minghuang Ling, Xiupian Liu, Longjiang Mao, Qiliang Lai, Zhuhua Luo","doi":"10.1099/ijsem.0.007323","DOIUrl":"https://doi.org/10.1099/ijsem.0.007323","url":null,"abstract":"<p><p>Two Gram-negative, aerobic, motile and rod-shaped bacterial strains, designated D34-3ᵀ and BC02-3-A7ᵀ, were isolated from deep-sea sediments of the eastern Pacific Ocean. Both strains were able to grow in the presence of 1.0-10.0% (wt/v) NaCl, at pH 6-9 and at 10-37 °C. The 16S rRNA gene sequence comparisons confirmed that these two isolates belonged to the genus <i>Christiangramia</i> of the family <i>Flavobacteriaceae,</i> exhibiting the highest 16S rRNA gene sequence similarity of D34-3ᵀ to <i>Christiangramia sediminicola</i> SM2212^T (98.8%) and BC02-3-A7ᵀ to <i>Christiangramia oceanisediminis</i> GC03-9ᵀ (97.2%), respectively. The complete genomes of D34-3ᵀ and BC02-3-A7ᵀ were 3.40 Mb (G+C content 37.5 mol%) and 4.43 Mb (G+C content 40.6 mol%) in size, with average nucleotide identity and digital DNA-DNA hybridization values with other reference <i>Christiangramia</i> species below 79.3/21.7% and 76.5/19.7%, respectively. Strain D34-3ᵀ contained menaquinone-6 as the sole respiratory quinone while menaquinone-7 was detected as the only respiratory quinone for BC02-3-A7ᵀ. These two strains possessed phosphatidylethanolamine as the dominant polar lipid. The predominant cellular fatty acids of both these strains were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (C_16 : 1 <i> ω</i>7<i>c</i> and/or C_16 : 1 <i> ω</i>6<i>c</i>) and summed feature 9 (iso-C_17 : 1 <i> ω</i>9<i>c</i> and/or 10-methyl C_16 : 0). Based on phenotypic, chemotaxonomic and genomic characteristics, strains D34-3ᵀ and BC02-3-A7ᵀ were identified as two novel species of the genus <i>Christiangramia</i>, for which the names <i>Christiangramia pacifica</i> sp. nov. and <i>Christiangramia profundi</i> sp. nov. are proposed; the type strains are D34-3ᵀ (=MCCC 1A13342^T=DSM 121736^T) and BC02-3-A7ᵀ (=MCCC M23243^T =DSM 121737^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149238376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Aquimarina ulvae</i> sp. nov. and <i>Polaribacter litoreus</i> sp. nov., isolated from the green alga <i>Ulva prolifera</i>.","authors":"Sylvia Kristyanto, Jiyoung Woo, Keun-Hyung Choi","doi":"10.1099/ijsem.0.007257","DOIUrl":"https://doi.org/10.1099/ijsem.0.007257","url":null,"abstract":"<p><p>Two Gram-negative, orange-pigmented, strictly aerobic, rod-shaped, catalase- and oxidase-positive bacterial strains, designated M1^T and M15^T, were isolated from the green alga <i>Ulva prolifera</i> collected in Muan Bay, Republic of Korea. Strain M1^T grew at 10-30 °C (optimum, 25 °C), pH 6-9 (optimum, pH 7), and in the presence of 1-6% (w/v) NaCl (optimum, 3%), whereas strain M15^T grew at 10-30 °C (optimum, 25 °C), pH 6-8 (optimum, pH 7), and with 1-5% (w/v) NaCl (optimum, 2%). The sole respiratory quinone in both strains is menaquinone-6. Major fatty acids in strain M1^T are iso-C_17 : 0 3-OH (27.8%), iso-C_15 : 0 (20.8%), summed feature 9 (C_16 : 0 10-methyl/iso-C_17 : 1 ω9c; 17.2%), and iso-C_15 : 1 G (13.4%); in strain M15^T, they are iso-C_15 : 0 (27.3%), iso-C_15 : 0 3-OH (16.0%), and C_15 : 1 ω6c (12.2%). The major polar lipids in strain M1^T were two unidentified aminolipids and seven unidentified lipids, whereas those in strain M15^T were phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminophospholipid, and four unidentified lipids. Phylogenetic analyses based on 16S rRNA gene nucleotide sequences revealed that strains M1^T and M15^T were most closely related to <i>Aquimarina muelleri</i> KMM 6020^T and <i>Polaribacter reichenbachii</i> 6Alg 8^T with 96.5 and 98.3% nucleotide sequence identities, respectively. The genomes of strains M1^T and M15^T were 5.5 Mb with a G+C content of 33.8 mol% and 4.1 Mb with a G+C content of 30.6 mol%, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between the genomic sequences of strain M1^T and <i>A. muelleri</i> KMM 6020^T were 74.2 and 19.4%, respectively, and between those of strain M15^T and <i>P. reichenbachii</i> 6Alg 8^T were 82.0 and 25.8%, respectively. Based on the polyphasic data, strains M1^T and M15^T are considered to represent novel species in the genera <i>Aquimarina</i> and <i>Polaribacter</i>, respectively, for which the names <i>Aquimarina ulvae</i> sp. nov. (M1^T=JCM 36757^T=KCTC 102217^T) and <i>Polaribacter litoreus</i> sp. nov. (M15^T=JCM 36759^T=KCTC 102246^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Sungouiella purgamenti</i> sp. nov., a yeast isolated from hospital wastewater in Brazil: a taxogenomic analysis.","authors":"Luiz Felipe A Santana, Katharina O Barros, Anna Paula O Tironi, Ana Raquel O Santos, Luiza B Bramusse, Karen Costa, Eloisa S Silva, Maria Clara V M Starling, Camila C Amorim, Daniela L Souza, Glória R Franco, Marc-André Lachance, Carlos A Rosa, Susana Johann","doi":"10.1099/ijsem.0.007279","DOIUrl":"https://doi.org/10.1099/ijsem.0.007279","url":null,"abstract":"<p><p>Yeasts recovered from wastewater of a public hospital in Minas Gerais state, Brazil, included a single isolate representing a putative novel species. Sequence analysis of the ITS-5.8S region and the D1/D2 domains of the LSU rRNA gene placed this yeast within the genus <i>Sungouiella</i>, close to <i>Sungouiella thailandica</i>. Phylogenomic inference based on 1,849 single-copy orthologues from <i>Sungouiella</i> species with available genomes confirmed that strain UFMG-CM-Y7468 forms a distinct lineage with <i>S. thailandica</i> as its closest known relative. The name <i>Sungouiella purgamenti</i> sp. nov. (MycoBank MB 863665) is proposed, with CBS 19634 designated as the holotype. The isolate was able to grow at 37 °C, showed susceptibility to most antifungal agents tested, except for itraconazole with an MIC of 0.5 mg l^-1, within the dose-dependent range, and exhibited strong extracellular protease activity. However, <i>S. purgamenti</i> showed a genomic toolkit consistent with a stress-tolerant and environmentally versatile lifestyle, while no specific or unique virulence determinants were identified based on genome content alone.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148919850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Proposal to replace the pro-illegitimate prokaryotic names '<i>Candidatus</i> Nostocoides' corrig. Kulichevskaya <i>et al</i>. 2012 and '<i>Candidatus</i> Nostocoides acidiphilum' corrig. Kulichevskaya <i>et al</i>. 2012 with '<i>Candidatus</i> Nostocimimus' gen. nov. and '<i>Candidatus</i> Nostocimimus acidiphilus' comb. nov.","authors":"Matthew B Crook","doi":"10.1099/ijsem.0.007287","DOIUrl":"https://doi.org/10.1099/ijsem.0.007287","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148911540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Demequina pilifera</i> sp. nov. and <i>Demequina ureilytica</i> sp. nov. isolated from a mixture of oil production fluids in Shengli Oilfield.","authors":"Yuwei Liu, Danni Wu, Ya-Jing Yu, Xin Song, Qiongyao Chen, Man Cai, Hong Ming","doi":"10.1099/ijsem.0.007278","DOIUrl":"https://doi.org/10.1099/ijsem.0.007278","url":null,"abstract":"<p><p>Two novel strains, designated SO4-18^T and SO4-13^T, were isolated from a mixture of oil production fluid sourced from Shengli Oilfield in China. Cells of the isolates were Gram-stain-positive, facultatively anaerobic, non-motile and piliated. Growth occurred at 4-37 °C (optimum 30 °C), pH 6.0-9.0 (optimum pH 8.0) and up to 0-13% (w/v) NaCl (optimum 1% and 1-2%, respectively). Phylogenetic analysis based on the 16S rRNA gene sequence, combined with genome-based investigations, revealed that the two isolates belong to the genus <i>Demequina</i>, and their 16S rRNA gene sequences shared less than 98.61% similarity with valid members in this genus. The average nucleotide identities, average amino acid identity and digital DNA-DNA hybridization values, whether between the two isolates or between either isolate and the type strains of other <i>Demequina</i> species, were all well beneath the established thresholds for species delineation. Pan-genomic analysis indicated that strains SO4-18^T and SO4-13^T shared ranges from 360 to 1,706 core gene clusters compared to other members of the genus <i>Demequina</i>. Chemotaxonomic characterization revealed the major polar lipids of the isolates, including diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, glycolipids and aminolipid, whereas the number and composition of unidentified lipids differed between the two isolates. The predominant fatty acids (>10% of the total content) were anteiso-C_15 : 0, C_16 : 0, anteiso-C_17 : 0 and anteiso-C_15 : 0 A, with menaquinone DMK-9(H₄) as the major respiratory quinones for both isolates. Based on the comprehensive phenotypic and genotypic characterizations presented in this study, strains SO4-18^T and SO4-13^T are proposed to represent two novel species of the genus <i>Demequina</i>, for which the names <i>Demequina pilifera</i> sp. nov. and <i>Demequina ureilytica</i> sp. nov. are proposed. The type strains of <i>Demequina pilifera</i> sp. nov. and <i>Demequina ureilytica</i> are SO4-18^T (=CGMCC 1.16447^T=KCTC 59404^T) and SO4-13^T (=CGMCC 1.16448^T=KCTC 59689^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Microbacterium wangyouchuni</i> sp. nov. and <i>Microbacterium zouquanmingi</i> sp. nov., isolated from soil in Mongolian marmot (<i>Marmota sibirica</i>) burrows.","authors":"Hongyu Yang, Han Zheng, Qun Xiao, Jing Yang, Wenbo Luo, Hao Zhou, Ji Pu, Jianguo Xu","doi":"10.1099/ijsem.0.007283","DOIUrl":"https://doi.org/10.1099/ijsem.0.007283","url":null,"abstract":"<p><p>Four Gram-stain-positive, aerobic, non-motile and rod-shaped bacterial strains, designated A82^T, A588, A196^T and A204, were isolated from soil samples collected from <i>Marmota sibirica</i> burrows in Hulun Buir, Nei Mongol Autonomous Region, China. All four strains grew at 4-30 °C, pH 6.0-10.0 and 0-6.5% (wt/v) NaCl, and they were positive for oxidase and catalase activities. Phylogenetic analysis of 16S rRNA gene sequences indicated that the four strains belonged to two species within the genus <i>Microbacterium</i>. Strains A82^T and A588 exhibited the highest sequence similarity to <i>Microbacterium profundi</i> Shh49^T (98.83%) and <i>Microbacterium murale</i> 1 Gi-001^T (98.67%). Strains A196^T and A204 were most closely related to <i>Microbacterium aerolatum</i> NBRC 103071^T (99.10%) and <i>Microbacterium shaanxiense</i> CCNWSP60^T (98.63%). Comparing the four isolates with their reference strains showed that average nucleotide identity and digital DNA-DNA hybridization values were below the accepted species-delineation benchmarks of 95-96 and 70%, respectively. For the type strains A82^T and A196^T, the DNA G+C content was 64.7 and 65.4 mol%, respectively. Chemotaxonomic analysis revealed that both type strains possessed MK-13 as the sole respiratory quinone. Additionally, diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids were the major polar lipids. Furthermore, <i>anteiso</i>-C_15 : 0 was identified as the major fatty acid, and the diamino acid in the cell-wall peptidoglycan contained alanine, glutamic acid, glycine, aspartic acid and ornithine. Therefore, strains A82^T/A588 and A196^T/A204 represent two novel species within the genus <i>Microbacterium</i>. <i>Microbacterium wangyouchuni</i> sp. nov. (A82^T/A588, type strain A82^T=GDMCC 1.5865^T=JCM 38342^T) and <i>Microbacterium zouquanmingi</i> sp. nov. (A196^T/A204, type strain A196^T=GDMCC 1.6024^T=JCM 38357^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148975856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Leifsonia araucanica</i> sp. nov.<i>,</i> isolated from nodules of <i>Lupinus luteus</i> and reclassification of <i>Leifsonia naganoensis</i> as a later heterotypic synonym of <i>Leifsonia aquatica</i>.","authors":"Grace Armijo-Godoy, Luis Cottet, Annally Rupayan, Makarena Carrasco, Daniela Levicoy, Haroldo Salvo-Garrido","doi":"10.1099/ijsem.0.007298","DOIUrl":"https://doi.org/10.1099/ijsem.0.007298","url":null,"abstract":"<p><p>A Gram-positive, non-spore-forming, rod-shaped actinobacterium, designated strain Le1^T, was isolated from root nodules of <i>Lupinus luteus</i> cultivated in volcanic soils of southern Chile. Phylogenetic analysis based on the 16S rRNA gene placed strain Le1ᵀ within the genus <i>Leifsonia</i>, showing the highest sequence similarity to <i>Leifsonia lichenia</i> and related species. However, genome-based analyses supported clear taxonomic separation. The draft genome of strain Le1ᵀ is 4.03 Mb, with a DNA G+C content of 69.26 mol%. Average nucleotide identity and digital DNA-DNA hybridization values between strain Le1ᵀ and its closest relatives ranged from 88.5 to 80.8% and 32.8 to 21.3%, respectively, below the accepted species delineation thresholds. Phylogenomic reconstruction based on 92 conserved core genes confirmed that strain Le1ᵀ forms a distinct and well-supported lineage within the genus. The predominant cellular fatty acids of strain Le1ᵀ are anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16 : 0. Phenotypic characteristics differentiate strain Le1ᵀ from its closest phylogenetic relatives. On the basis of polyphasic taxonomic evidence, strain Le1ᵀ represents a novel species of the genus <i>Leifsonia</i>, for which the name <i>Leifsonia araucanica</i> sp. nov. is proposed. The type strain is Le1ᵀ (=RGM 3741ᵀ=LMG 34089ᵀ). In addition, genome-based comparisons indicate that <i>Leifsonia naganoensis</i> Suzuki <i>et al</i>. 2000 should be regarded as a later heterotypic synonym of <i>Leifsonia aquatica</i> (ex Leifson 1962) Evtushenko <i>et al</i>. 2000.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}