International journal of systematic and evolutionary microbiology最新文献

{"title":"Two new members of the family <i>Aerococcaceae</i>: <i>Tuanshanicoccus yangjingiae</i> gen. nov., sp. nov. and <i>Tuanshanicoccus lijuaniae</i> sp. nov., isolated from the trachea of <i>Marmota himalayana</i>.","authors":"Gui Zhang, Yanpeng Cheng, Yue Liu, Dong Jin, Shan Lu, Weiguang Li, Ji Pu, Yajun Ge, Caiyun Ma, Wenbo Luo, Jianguo Xu","doi":"10.1099/ijsem.0.006911","DOIUrl":"https://doi.org/10.1099/ijsem.0.006911","url":null,"abstract":"<p><p>Nine strains of two Gram-positive, facultative anaerobic, catalase-, oxidase- and urease-negative coccoid species, designated as zg-252^T, zg-1578, zg-B36, zg-ZUI334, and zg-1292^T, zg-A91, zg-BR9, zg-BR22 and zg-BR33, were isolated from the trachea of <i>Marmota himalayana</i>. The nine strains grew optimally at 37 °C, pH 7.0 and 0.5% NaCl. Comparative analysis of the 16S rRNA gene revealed that the two novel type strains were most closely related to each other, with low similarity to known species in the family <i>Aerococcaceae</i>. Phylogenetic analyses based on the 16S rRNA gene and genome suggested that the nine strains form two independent branches, well separated from all genera of this family. The genomic G+C contents of strains zg-252^T and zg-1292^T were 36.0 mol% and 37.8 mol%. Comparative genomic analysis proved that the nine strains represent two different novel species of a new genus. The major fatty acids of this novel genus were C_18 : 1 <i> ω</i>9c and C_16 : 0, with diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids, but no quinones were detected. The two novel species shared cell-wall aa (glutamic acid, alanine and lysine) and cell-wall sugars (ribose; with additional glucose in zg-1292^T). Based on the polyphasic analysis, we proposed that the unknown bacteria can be classified as <i>Tuanshanicoccus</i> gen. nov., with two species in this novel genus, type species <i>Tuanshanicoccus yangjingiae</i> gen. nov., sp. nov. (zg-252^T = GDMCC 1.1725^T = JCM 34096^T) and <i>Tuanshanicoccus lijuaniae</i> sp. nov. (zg-1292^T = GDMCC 1.2118^T = KCTC 43284^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactic acid production of bacteria from tree barks and proposal of <i>Sporolactobacillus caesalpiniae</i> sp. nov.","authors":"Jenjuiree Mahittikon, Sitanan Thitiprasert, Nuttha Thongchul, Naoto Tanaka, Yuh Shiwa, Nitcha Chamroensaksri, Wongsakorn Phongsopitanun, Somboon Tanasupawat","doi":"10.1099/ijsem.0.006915","DOIUrl":"10.1099/ijsem.0.006915","url":null,"abstract":"<p><p>Lactic acid bacteria are widely exploited in biotechnology due to their fermentation capacity and metabolite production. In this study, 19 isolates were recovered from tree bark collected across Thailand, representing the genera <i>Enterococcus</i> (11 isolates), <i>Lactococcus</i> (2 isolates), <i>Weizmannia</i> (3 isolates), <i>Sporolactobacillus</i> (2 isolates) and <i>Terrilactibacillus</i> (1 isolate). All exhibited homofermentative metabolism, producing l-, dl- or d-lactic acid. Among them, strain STCC-11^T, isolated from the bark of <i>Caesalpinia coriaria</i> in Satun Province, Thailand, demonstrated strong potential for d-lactic acid production, achieving 96.31% optical purity, 1.082 g l^-1 concentration and 0.451 g g^-1 glucose yield. This novel Gram-positive, spore-forming, facultatively anaerobic bacterium STCC-11^T grew at 30-45 °C, pH 6.0-9.0 and up to 3% NaCl. Phylogenetic analysis of the 16S rRNA gene revealed 99.71% similarity with <i>Sporolactobacillus nakayamae</i> subsp. <i>nakayamae</i> ATCC 700379^T. In addition, whole-genome analyses indicated that STCC-11^T is distinct, with average nucleotide identity based on BLAST (ANIb) (94.12%), average nucleotide identity based on MUMmer (ANIm) (95.29%) and digital DNA-DNA hybridization (dDDH) (60.3%) values below species delineation thresholds. The genome (3.57 Mb, 43.8% G+C) encodes both <i>ldhD</i> and <i>ldhL</i> genes, and <i>in silico</i> safety assessment confirmed the absence of pathogenicity or transferable resistance genes. Based on polyphasic taxonomic evidence, STCC-11^T represents a novel species of the genus <i>Sporolactobacillus</i>, for which the name S<i>porolactobacillus caesalpiniae</i> sp. nov. is proposed. The type strain is STCC-11^T (LMG 33980^T <b>=</b>TISTR 10806^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Butyricimonas muris</i> sp. nov., isolated from mouse faeces.","authors":"Tong Xu, Eryi Ju, Zhuo A Wang, Jinhua Wei, Yujing Wang, Yuguang Du","doi":"10.1099/ijsem.0.006906","DOIUrl":"https://doi.org/10.1099/ijsem.0.006906","url":null,"abstract":"<p><p>An extracellular polysaccharide-producing strain labelled GBG-M4^T was isolated from the faeces of a C57BL/6 J mouse model with ulcerative colitis prognosis. Using a polyphasic approach, the taxonomy of this novel species was characterized. The strain was Gram-negative, non-motile, non-spore-forming, non-pigmented and rod-shaped. Strain GBG-M4 ^T grew anaerobically at 30-45℃ (optimally at 37-40℃) and at pH 6.5-8.0 (optimally at pH 7.0-8.0). Growth of the strain was not inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that strain GBG-M4^T represents a member of the genus <i>Butyricimonas</i>. The similarity of the 16S rRNA gene sequence of strain GBG-M4^T to the type species of the genus, <i>Butyricimonas faecihominis</i> JCM 18676^T, was 94.56%. This strain could produce acetic acid, propionic acid, butyric acid, iso-butyric acid, valeric acid and caproic acid as final products from glucose. The major polar lipids were five lipids, three phospholipids, four ammonium phosphatides and one glyceride. The major fatty acids of strain GBG-M4^T were iso-C _{17: 0} 3-OH and iso-C _{15: 0}. Genome analysis revealed that strain GBG-M4^T has a genome size of 5.56 Mb and a DNA G+C content of 45.57 mol%. A total of 4,576 genes were predicted by genome sequence analysis. On the basis of phenotypic, chemotaxonomic and phylogenetic characterization, strain GBG-M4^T represents a novel species within the genus <i>Butyricimonas</i>, for which the name <i>Butyricimonas muris</i> sp. nov. is proposed. The type strain is GBG-M4^T (=CGMCC 1.18113^T=KCTC 25905^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bergeyella anatis</i> sp. nov., isolated from the upper respiratory tract of duck.","authors":"Yuhao Liu, Yulong Dai, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu","doi":"10.1099/ijsem.0.006901","DOIUrl":"https://doi.org/10.1099/ijsem.0.006901","url":null,"abstract":"<p><p>Five bacterial strains, designated as RCAD1438, RCAD1439^T, RCAD1670, RCAD1671 and RCAD1672, were isolated from the upper respiratory tract of ducks in Anhui, Shaanxi and Sichuan, China. All strains are Gram-stain-negative, rod-shaped, non-motile, non-spore-forming, aerobic and capsulated. They grow optimally at 37 °C and pH 7.5 and are positive for oxidase and catalase activities. High 16S rRNA gene sequence similarity (>99%) and average nucleotide identity (ANI) values (>96%) confirm that the five strains belong to the same species. Phylogenetic analysis based on 16S rRNA gene sequences indicates that these strains belong to the genus <i>Bergeyella</i> and are closely related to <i>Bergeyella porcorum</i> CECT 9006^T (96.7 %-97.1%) and <i>Bergeyella zoohelcum</i> NBRC 16014^T (96.7 %-97.0%). However, ANI and digital DNA-DNA hybridization values between strain RCAD1439^T and these two type strains, as well as other closely related strains, were no more than 72.0% and 35.0%, respectively, both below the accepted species thresholds. The genomic size of the five strains is 2.4-2.6 Mb, with genomic DNA G+C content of 42.1-42.9 mol%. The major respiratory quinone of strain RCAD1439^T was menaquinone MK-6, and its polar lipid profile includes phosphatidylethanolamine, two unidentified aminolipids, one unidentified aminolipid and six unidentified lipids. Based on physiological, genotypic, phylogenetic and chemotaxonomic data, these five strains represent a novel species of <i>Bergeyella</i>, for which the name <i>Bergeyella anatis</i> sp. nov. is proposed. The type strain is RCAD1439^T (=CCTCC AB 2024048^T=GDMCC 1.4426^T=KCTC 102274^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Psychromonas algicola</i> sp. nov. and <i>Psychromonas algarum</i> sp. nov., two novel bacteria isolated from red macroalgae (<i>Grateloupia</i> sp.).","authors":"Si-Jia Fan, Han-Zhe Zhang, Bei Pan, Tian-He Liu, De-Chen Lu, Zong-Jun Du","doi":"10.1099/ijsem.0.006903","DOIUrl":"https://doi.org/10.1099/ijsem.0.006903","url":null,"abstract":"<p><p>Two Gram-stain-negative and rod-shaped bacteria, designated as RZ5^T and RZ22^T, isolated from a red macroalgae sample, were characterized by a polyphasic approach to clarify their taxonomic position. Strain RZ5^T grew at 4-33 °C (optimum, 25-28 °C), pH 6.5-8.5 (optimum, 7.0-8.0) and in 0.5-5.0% (w/v) NaCl (optimum, 1.0%). Strain RZ22^T grew at 4-28 °C (optimum, 20-25 °C), pH 6.5-8.5 (optimum, 7.0-8.0) and in 2.0-5.0% (w/v) NaCl (optimum, 3.0%). Strain RZ5^T possessed a flagellum and was motile, while strain RZ22^T lacked a flagellum and was non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both isolates belonged to the genus <i>Psychromonas</i> and were most closely related to <i>Psychromonas aquatilis</i> M1A1^T (98.1%) and <i>Psychromonas antarctica</i> DSM 10704^T (97.1%) for strains RZ5^T and RZ22^T, respectively. The predominant fatty acids of both strains were C_16 : 0 and summed feature 3 (C_16 : 1 <i> ω</i>7<i>c</i>/C_16 : 1 <i> ω</i>6<i>c</i>). The major polar lipids for strain RZ5^T comprised phosphatidylethanolamine (PE) and glycolipids (GLs), while those for strain RZ22^T comprised PE, diphosphatidylglycerol and GLs. The average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains RZ5^T and RZ22^T were 85.4% and 18.9%, respectively. Strain RZ5^T had ANI values of 84.3-85.5% and dDDH values of 18.3-21.3% to members of the genus <i>Psychromonas</i>. Strain RZ22^T had ANI values of 84.2-85.2% and dDDH values of 18.4-22.1% to members of the genus <i>Psychromonas</i>. The G+C content of the genomic DNA of strains RZ5^T and RZ22^T was 36.7 mol% and 35.6 mol%, respectively. Strains RZ5^T and RZ22^T possessed ubiquinone-8 as the sole respiratory quinone. The phylogenetic analyses and genomic comparisons, combined with phenotypic and chemotaxonomic features, strongly supported that the two strains should be classified as representing two novel species of the genus <i>Psychromonas</i>, for which we propose the names <i>Psychromonas algicola</i> sp. nov. (type strain RZ5^T=JCM 33153^T=MCCC 1H00360^T) and <i>Psychromonas algarum</i> sp. nov. (type strain RZ22^T=JCM 33154^T=MCCC 1H00361^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Aurantivibrio plasticivorans</i> gen. nov., sp. nov. and <i>Aurantivibrio infirmus</i> sp. nov., biodegradable plastic-degrading bacteria belonging to the family <i>Cellvibrionaceae</i>, isolated from the coast of Japan.","authors":"Takamasa Miura, Rieko Kasaishi, Kohei Hidaka, Mamiko Shimamura, Moriyuki Hamada, Tomoyo Miyakawa, Yoko Kusuya, Yoshiyuki Ishitani, Shun'ichi Ishii, Yoko Furuno, Risa Yokoyama, Dai-Ichiro Kato, Asuka Arimoto, Kazuya Fukuda, Tatsuya Ueki, Kunifumi Tagawa, Yoshihito Uchino, Kei Kamino","doi":"10.1099/ijsem.0.006920","DOIUrl":"10.1099/ijsem.0.006920","url":null,"abstract":"<p><p>Two Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterial strains, designated 4NK60-0013^T and 4NH20-0068^T, were isolated from biodegradable plastic films immersed in the coastal waters of Japan. These strains showed the ability to degrade biodegradable plastics, poly(butylene succinate-<i>co</i>-adipate) (PBSA) and poly(<i>ε</i>-caprolactone). Phylogenetic analyses based on the 16S rRNA gene sequences revealed that strains 4NK60-0013^T and 4NH20-0068^T belong to the family <i>Cellvibrionaceae</i> in the order <i>Cellvibrionales</i> and are closely related to members of the genus <i>Gilvimarinus</i>. The species most closely related to strains 4NK60-0013^T and 4NH20-0068^T were <i>Gilvimarinus agarilyticus</i> M5c^T and <i>Gilvimarinus polysaccharolyticus</i> YN3^T, with 16S rRNA gene sequence similarities of 94.4 and 93.3%, respectively. The sequence similarity between the two novel strains was 94.8%, indicating that they represented separate species. The genome length and G+C content of the genome sequence of strain 4NK60-0013^T were 4,354,061 bp and 46.9 mol%, respectively, whereas those of strain 4NH20-0068^T were 4,154,637 bp and 43.3 mol%, respectively. The average nucleotide identity values between the strains were <72.0%, similar to the values between the strains and type strains of species from the genera <i>Cellvibrio</i>, <i>Gilvimarinus</i> and <i>Marinimicrobium</i>. Based on phenotypic, cellular fatty acid and phylogenetic characteristics, the two isolates represent novel species within a new genus of the family <i>Cellvibrionaceae</i>, for which the names <i>Aurantivibrio plasticivorans</i> gen. nov., sp. nov. (type species) and <i>Aurantivibrio infirmus</i> sp. nov. have been proposed. Additionally, these strains and other bacteria belonging to the family <i>Cellvibrionaceae</i> have been shown to biodegrade PBSA, a biodegradable plastic. The type strains of <i>A. plasticivorans</i> and <i>A. infirmus</i> are 4NK60-0013^T (=NBRC 116180^T=TBRC 19146^T) and 4NH20-0068^T (=NBRC 116181^T=TBRC 19147^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cyberlindnera hibernica</i> sp. nov. and <i>Barnettozyma discipulorum</i> sp. nov., isolated from forest soil in Ireland.","authors":"Adam P Ryan, Cláudia Carvalho, Yiran Zhao, Julianna Decuseara, Matthieu Osborne, Padraic G Heneghan, Kevin P Byrne, Tadhg Ó Cróinín, Kenneth H Wolfe, José Paulo Sampaio, Geraldine Butler","doi":"10.1099/ijsem.0.006898","DOIUrl":"10.1099/ijsem.0.006898","url":null,"abstract":"<p><p>Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the <i>Cyberlindnera</i> and <i>Barnettozyma</i> genera of the order <i>Phaffomycetales</i>, but they did not exactly match any known species. The genomes of both isolates were sequenced using Oxford Nanopore Technologies and Illumina sequencing, generating chromosome-level genome assemblies. Phylogenomic analysis of 1,385 single-copy orthologues from 37 <i>Phaffomycetales</i> species and 2 outgroups showed that the closest relative of PYCC 10015 is <i>Cyberlindnera galapagoensis</i> and that PYCC 10016 is placed in a subclade containing 7 other species from the <i>Barnettozyma</i> genus. The average nucleotide identity between these strains and their closest relatives is <75%, supporting their designation as novel species. Here, we propose the names <i>Cyberlindnera hibernica</i> sp. nov. and <i>Barnettozyma discipulorum</i> sp. nov. for PYCC 10015 and PYCC 10016, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Methylobacterium alsaeris</i> sp. nov. and <i>Methylobacterium carpenticola</i> sp. nov., isolated from a car air conditioning system.","authors":"Jung Pyo Yoon, Tae Hun Kim, So Yoon Park, Ki Young Yoon, Hyung Min Kim","doi":"10.1099/ijsem.0.006884","DOIUrl":"10.1099/ijsem.0.006884","url":null,"abstract":"<p><p>Two novel pink-pigmented bacteria, designated strains MA0201^T and ID0610^T, were isolated from a car air conditioning system. The isolates were Gram-stain-negative, aerobic, non-spore-forming, rod-shaped and facultatively methylotrophic. Cells of strains MA0201^T and ID0610^T are motile with polar flagella (lophotrichous) and a polar flagellum (monotrichous), respectively. Both strains indicated catalase- and oxidase-positive activities. The growth of strain MA0201^T was observed at 15-35 °C, at pH 5.0-7.5 and in the presence of 0-0.5% (w/v) NaCl, while the growth of strain ID0610^T indicated at 20-40 °C, at pH 5.0-7.5 and in the presence of 0-0.5% (w/v) NaCl. Ubiquinone-10 was the major respiratory quinone in both strains. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were identified as the major polar lipids in both strains, and an unidentified lipid and an unidentified aminolipid were additionally identified in strains MA0201^T and ID0610^T, respectively. The major cellular fatty acids (>5%) were summed feature 8 (C_18 : 1 <i> ω7</i>c and/or C_18 : 1 <i> ω6</i>c) and C_19 : 0 cyclo <i>ω</i>8<i>c</i> in strain MA0201^T, while strain ID0610^T contained summed feature 8 (C_18 : 1 <i> ω7</i>c and/or C_18 : 1 <i> ω6</i>c) and C_18 : 0. Phylogenetic and phylogenomic analyses showed that strains MA0201^T and ID0610^T formed distinct phylogenic lineages within the genus <i>Methylobacterium</i>. Strain MA0201^T exhibited high 16S rRNA gene sequence similarity to <i>Methylobacterium platani</i> PMB02^T (98.8%), and digital DNA-DNA hybridization (dDDH) and orthologous average nt identity (ANI) values were 38.8% and 90.0%, respectively. Strain ID0610^T showed high 16S rRNA gene sequence similarity to <i>Methylobacterium isbiliense</i> AR24^T (98.5%), and dDDH and ANI values were 27.2% and 83.4%, respectively, indicating that both strains represent novel species of the genus <i>Methylobacterium</i>. Based on their phenotypic, chemotaxonomic and molecular properties, strains MA0201^T and ID0610^T are proposed to represent two novel species within the genus <i>Methylobacterium</i>, namely, <i>Methylobacterium alsaeris</i> sp. nov. (type strain MA0201^T=KACC 23846^T=JCM 37404^T) and <i>Methylobacterium carpenticola</i> sp. nov. (type strain ID0610^T=KACC 23847^T=JCM 37403^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of <i>Paucibacter soli</i> sp. nov. and <i>Paucibacter hankyongi</i> sp. nov. isolated from wetland soil.","authors":"Muhammad Zubair Siddiqi, Wan-Taek Im","doi":"10.1099/ijsem.0.006899","DOIUrl":"https://doi.org/10.1099/ijsem.0.006899","url":null,"abstract":"<p><p>Two bacterial strains, designated as AS307^T and AS339^T, were isolated from wet soil in South Korea. The genome analysis shows that strains AS307^T and AS339^T belong to the genus <i>Paucibacter</i> and share the highest 16S rRNA gene similarity (≥97.9%) with <i>Paucibacter oligotrophus</i> KCTC 42519^T and '<i>Paucibacter aquatile</i>' KCCM 90284^T, respectively. The draft genome of strains AS307^T and AS339^T consists of circular chromosomes of 5,374,046 and 5,098,490 bp with DNA G+C content of 67.6 mol% and 62.7 mol%, respectively. Strains AS307^T and AS339^T show the ubiquinone 8 as the major quinone, C_16 : 0, C_16 : 1 <i> ω7</i>c/_C16 : 1 <i> ω</i>6<i>c</i> (summed feature 3) as major fatty acids and phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine as major polar lipids. The average nt identity and digital DNA-DNA hybridization values between strains AS307^T and AS339^T and phylogenetically closest strains were less than 80.5% and 19.0-22.5%, respectively. Furthermore, the genomic, physiological and biochemical results allow the phenotypic and genotypic differentiation of strains AS307^T and AS339^T from their closest and other species of the genus <i>Paucibacter</i> with validly published names. Therefore, both strains AS307^T and AS339^T represent novel species of the genus <i>Paucibacter</i>, for which the names <i>Paucibacter soli</i> sp. nov. (type strain AS307^T=KACC 23091^T=LMG 32952^T) and <i>Paucibacter hankyongi</i> sp. nov. (type strain AS339^T=KACC 23092^T=LMG 32953^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Guyparkeria halopsychrophila</i> sp. nov., a chemolithoautotrophic polyextremophile isolated from a sub-zero Arctic hypersaline spring.","authors":"Matthew W Quinn, Olivia Blenner-Hassett, Scott Sugden, Lochlan Breckenridge, Lyle G Whyte","doi":"10.1099/ijsem.0.006883","DOIUrl":"10.1099/ijsem.0.006883","url":null,"abstract":"<p><p>Two eurypsychrophilic and halophilic sulphur-oxidizing bacterial strains (LHSS19-1^T and GHLCS8-2) were isolated from High Arctic hypersaline sulphur spring sediment samples collected from the Lost Hammer and Gypsum Hill spring systems on Axel Heiberg Island in the Qikiqtaaluk Region, Nunavut, Canada. The strains were obligate chemolithoautotrophs, utilizing reduced sulphur compounds (thiosulphate, sulphide, tetrathionate and elemental sulphur) as electron donors and energy sources. Chemolithoautotrophic growth on thiosulphate was observed at -2 to 37 °C, pH 6.5-9.5 and NaCl concentrations of 1-20 % w/v. The DNA G+C content of the draft genome sequences was 64.59-64.74 mol%. Phylogenetic analyses based on 16S rRNA gene sequences, 92 single-copy core genes and whole genomes indicated that both strains together form a distinct monophyletic clade within the genus <i>Guyparkeria</i>. Moreover, <i>in silico</i> DNA-DNA hybridization, orthologous average nt identity and average aa identity values calculated between each strain and the closest related type strains were ≤69.10%, 87.83% and 90.37%, respectively. The genomes of both strains encoded high O₂ affinity <i>cbb3</i>-type cytochrome c oxidase genes but distinctively lacked a complete set of <i>aa3</i>-type cytochrome c oxidase genes. The dominant respiratory quinone of strain LHSS19-1^T was ubiquinone-8. The major fatty acids (>10%) were C_18 : 1  <i>ω</i>7c, C_12 : 0 3OH and C_16 : 0. Based on phenotypic and genotypic results, strains LHSS19-1^T and GHLCS8-2 represent a novel species within the genus <i>Guyparkeria</i>, for which the name <i>Guyparkeria halopsychrophila</i> sp. nov. is proposed. The type strain is LHSS19-1^T (=NCTC 15100^T=DSM 117890^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}