International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Thioclava arctica</i> sp. nov. and <i>Zhongshania arctica</i> sp. nov., isolated from the surface sediment of the Arctic Ocean and reclassification of <i>Marortus luteolus</i> Yu <i>et al.</i> 2019 as a later heterotypic synonym of <i>Zhongshania marina</i> On <i>et al.</i> 2019.","authors":"Panyi Pan, Jianning Wang, Xiupian Liu, Qiliang Lai, Guangyu Li, Yaping Du, Jinzhu Zhang, Ziyuan He, Zongze Shao, Chunming Dong","doi":"10.1099/ijsem.0.006548","DOIUrl":"https://doi.org/10.1099/ijsem.0.006548","url":null,"abstract":"<p><p>Two Gram-stain-negative bacterial strains, 15-R06ZXC-3^T and R06B22^T, were isolated from the surface sediment of the Arctic Ocean. Phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that strain 15-R06ZXC-3^T belongs to the genus <i>Thioclava</i>, while strain R06B22^T belongs to the genus <i>Zhongshania</i>. Strain 15-R06ZXC-3^T showed the closest relationship to <i>Thioclava indica</i> DT23-4^T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 15-R06ZXC-3^T and all of the type strains of the genus <i>Thioclava</i> ranged from 20.8 to 30.4% and 79.1 to 85.7%, respectively. Strain R06B22^T was most closely related to <i>Zhongshania marina</i> DSW25-10^T. The dDDH and ANI values between strain R06B22^T and all of the type strains of the genus <i>Zhongshania</i> ranged from 18.6 to 20.4% and 77.5 to 79.4%, respectively. These dDDH and ANI values were all below the standard cutoff criteria for the delineation of bacterial species, indicating that the two strains may represent novel species within their respective genera. Furthermore, their phenotypic and chemotaxonomic characteristics also differentiated them from closely related species. Based on the polyphasic analyses, strains 15-R06ZXC-3^T and R06B22^T separately represent novel species of the genera <i>Thioclava</i> and <i>Zhongshania</i>, for which the names <i>Thioclava arctica</i> sp. nov. (type strain 15-R06ZXC-3^T = MCCC 1A07434^T= KCTC 8342^T) and <i>Zhongshania arctica</i> sp. nov. (type strain R06B22^T = MCCC 1A08273^T= KCTC 8343^T) are proposed. Additionally, phylogenomic analyses showed that the strain <i>Marortus luteolus</i> ZX-21^T was clustered with the strain <i>Z. marina</i> DSW25-10^T and all other type strains of the genus <i>Zhongshania</i>. Furthermore, the ANI and dDDH values between strains ZX-21^T and DSW25-10^T were 97.6% and 78.8±2.5%, respectively, strongly indicating that they represented a single species. Therefore, it is proposed that <i>M. luteolus</i> Yu <i>et al.</i> 2019 be recognized as a later heterotypic synonym of <i>Z. marina</i> On <i>et al.</i> 2019.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Sodalis praecaptivus</i> subsp. <i>spalangiae</i> subsp. nov., a nascent bacterial endosymbiont isolated from the parasitoid wasp, <i>Spalangia cameroni</i>.","authors":"Li Szhen Teh, Sarit Rohkin Shalom, Ian James, Anna Dolgova, Elad Chiel, Colin Dale","doi":"10.1099/ijsem.0.006552","DOIUrl":"https://doi.org/10.1099/ijsem.0.006552","url":null,"abstract":"<p><p>An endosymbiotic bacterium of the genus <i>Sodalis</i>, designated as strain HZ^T, was cultured from the parasitoid wasp <i>Spalangia cameroni</i>, which develops on the pupae of various host flies. The bacterium was detected in <i>S. cameroni</i> developed on houseflies, <i>Musca domestica</i>, in a poultry facility in Hazon, northern Israel. After culturing, this bacterium displayed no surface motility on Luria-Bertani agar and was rod-shaped and irregular in size, ~10-30 nm in diameter and 5-20 µm in length. Phylogenetic analyses revealed that strain HZ^T is closely related to <i>Sodalis praecaptivus</i> strain HS^T, a free-living species of the genus <i>Sodalis</i> that includes many insect endosymbionts. Although these bacteria maintain >98% sequence identity in shared genes, genomic characterization revealed that strain HZ^T has undergone substantial reductive evolution, such that it lacks many gene functions that are maintained in <i>S. praecaptivus</i> strain HS^T. Based on the results of phylogenetic, genomic and chemotaxonomic analyses, we propose that this endosymbiont should be classified in a new subspecies as <i>S. praecaptivus</i> subsp. s<i>palangiae</i> subsp. nov. The type strain for this new subspecies is HZ^T (=ATCC TSD-398^T=NCIMB 15482^T). The subspecies <i>Sodalis praecaptivus</i> subsp. <i>praecaptivus</i> strain HS^T is created automatically with the type strain ATCC BAA-2554^T (=DSMZ 27494^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paenibacillus suaedae</i> sp. nov. and <i>Paenibacillus violae</i> sp. nov., isolated from the roots of <i>Suaeda japonica</i> Makino and <i>Viola mandshurica</i> W. Becker with plant growth-promoting potential.","authors":"Inhyup Kim, Sunho Park, Yonghee Jung, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo","doi":"10.1099/ijsem.0.006542","DOIUrl":"https://doi.org/10.1099/ijsem.0.006542","url":null,"abstract":"<p><p>Two novel Gram-positive strains, chi10^T and PFR10^T, were isolated from the roots of <i>Suaeda japonica</i> Makino and <i>Viola mandshurica</i> W. Becker, respectively. The strains are facultatively aerobic, rod-shaped, motile via peritrichous flagella and endospore-forming. Strains chi10^T and PFR10^T showed the highest 16S rRNA gene sequence similarity to <i>Paenibacillus alvei</i> DSM 29^T (99.0%) and <i>Paenibacillus planticolens</i> LMG 31457^T (99.3%). Furthermore, the phylogenetic and phylogenomic analyses demonstrated that strains chi10^T and PFR10^T were closely related to the genus <i>Paenibacillus</i>. The digital DNA-DNA hybridization (dDDH) values for strains used in the phylogenetic analysis and strain chi10^T ranged from 19.6 to 28.4%, while for strain PFR10^T, the values ranged from 19.8 to 53.5%, according to the Genome-to-Genome Distance Calculator 3.0, which were all less than 70%. Additionally, the dDDH value between strains chi10^T and PFR10^T was 22.0%, confirming that they are different species of the genus <i>Paenibacillus</i>. The average nucleotide identity between chi10^T and PFR10^T and other species of the genus <i>Paenibacillus</i> were 68.4-84.7% and 67.8-93.6%, respectively. In addition, the major polar lipids of chi10^T and PFR10^T consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acid profile of strain chi10^T was iso-C_15 : 0 and anteiso-C_15 : 0, whereas the major fatty acid profile of strain PFR10^T was iso-C_15 : 0, anteiso-C_15 : 0 and iso-C_16 : 0. The sole respiratory quinone in strains chi10^T and PFR10^T was menaquinone-7. Phylogenomic, phenotypic and genome analyses strongly indicated that chi10^T and PFR10^T could be two novel <i>Paenibacillus</i> species for which the names <i>Paenibacillus suaedae</i> sp. nov. (type strain chi10^T = KACC 23258^T = TBRC 17803^T) and <i>Paenibacillus violae</i> sp. nov. (type strain PFR10^T = KACC 23263^T = TBRC 17804^T) are proposed, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposal of <i>Allocatenococcus</i> gen. nov., <i>Allocoenonia</i> gen. nov. and <i>Allofournierella</i> gen. nov. as replacement names for the illegitimate prokaryotic generic names <i>Catenococcus</i> Sorokin 1994, <i>Coenonia</i> Vandamme <i>et al</i>. 1999 and <i>Fournierella</i> Togo <i>et al</i>. 2017, respectively.","authors":"Aharon Oren, Eduardo A Molinari Novoa","doi":"10.1099/ijsem.0.006547","DOIUrl":"https://doi.org/10.1099/ijsem.0.006547","url":null,"abstract":"<p><p>The prokaryotic generic names <i>Catenococcus</i> Sorokin 1994, <i>Coenonia</i> Vandamme <i>et al</i>. 1999 and <i>Fournierella</i> Togo <i>et al</i>. 2017 are illegitimate because they are later homonyms of <i>Catenococcus</i> Hindák 1977, <i>Coenonia</i> Van Tieghem 1884 and <i>Fournierella</i> Collignon 1966, respectively (Principle 2 and Rules 51b(5) and 51b(4) of the International Code of Nomenclature of Prokaryotes). We, therefore, propose the replacement generic names <i>Allocatenococcus</i>, <i>Allocoenonia</i> and <i>Allofournierella</i>, with type species <i>Allocatenococcus thiocycli</i>, <i>Allocoenonia anatina</i> and <i>Allofournierella massiliensis</i>, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Kondoa tianchiensis</i> f.a., sp. nov., an anamorphic yeast species isolated from plant leaves.","authors":"Dan-Yang Cai, Shan Liu, Feng-Li Hui","doi":"10.1099/ijsem.0.006487","DOIUrl":"10.1099/ijsem.0.006487","url":null,"abstract":"<p><p>Two yeast strains, NYNU 236122 and NYNU 236180, were isolated from plant leaves collected in Tianchi Mountain, Henan Province, central China. Molecular phylogenetic analyses revealed the closest relatives of the strains are three described <i>Kondoa</i> species, <i>Kondoa chamaenerii</i>, <i>Kondoa miscanthi</i>, and <i>Kondoa subrosea</i>. Genetically, the isolated strains differed from the type strains of their three related species by 2-11(0.2-1.8%) base substitutions in the D1/D2 domain, 16-40 (2.6-5.6%) base mismatches in the internal transcribed spacer region, and more than 10.1% base substitutions in the partial <i>RPB2</i> gene. Furthermore, the two strains differ physiologically from their closest related species, <i>K. chamaenerii</i>, in their ability to assimilate dl-lactate, nitrite, and l-lysine and their inability to assimilate nitrate. Additionally, they differ from <i>K. miscanthi</i> and <i>K. subrosea</i> in their ability to assimilate inulin, d-gluconate, and l-lysine. The species name of <i>Kondoa tianchiensis</i> f.a., sp. nov. is proposed with holotype CICC 33616^T (Mycobank MB 853544).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptomyces castrisilvae</i> sp. nov. and <i>Streptomyces glycanivorans</i> sp. nov., novel soil streptomycetes metabolizing mutan and alternan.","authors":"Tove Widén, Albert Tafur Rangel, Vincent Lombard, Elodie Drula, Scott Mazurkewich, Nicolas Terrapon, Eduard J Kerkhoven, Johan Larsbrink","doi":"10.1099/ijsem.0.006514","DOIUrl":"10.1099/ijsem.0.006514","url":null,"abstract":"<p><p>Six bacterial strains, Mut1^T, Mut2, Alt1, Alt2, Alt3^T, and Alt4, were isolated from soil samples collected in parks in Gothenburg, Sweden, based on their ability to utilize the insoluble polysaccharides α-1,3-glucan (mutan; Mut strains) or the mixed-linkage α-1,3/α-1,6-glucan (alternan; Alt strains). Analysis of 16S rRNA gene sequences identified all strains as members of the genus <i>Streptomyces</i>. The genomes of the strains were sequenced and subsequent phylogenetic analyses identified Mut2 as a strain of <i>Streptomyces laculatispora</i> and Alt1, Alt2 and Alt4 as strains of <i>Streptomyces poriferorum,</i> while Mut1^T and Alt3^T were most closely related to the type strains <i>Streptomyces drozdowiczii</i> NBRC 101007^T and <i>Streptomyces atroolivaceus</i> NRRL ISP-5137^T, respectively. Comprehensive genomic and biochemical characterizations were conducted, highlighting typical features of <i>Streptomyces</i>, such as large genomes (8.0-9.6 Mb) with high G+C content (70.5-72.0%). All six strains also encode a wide repertoire of putative carbohydrate-active enzymes, indicating a capability to utilize various complex polysaccharides as carbon sources such as starch, mutan, and cellulose, which was confirmed experimentally. Based on phylogenetic and phenotypic characterization, our study suggests that strains Mut1^T and Alt3^T represent novel species in the genus <i>Streptomyces</i> for which the names <i>Streptomyces castrisilvae</i> sp. nov. and <i>Streptomyces glycanivorans</i> sp. nov. are proposed, with strains Mut1^T (=DSM 117248^T=CCUG 77596^T) and Alt3^T (=DSM 117252^T=CCUG 77600^T) representing the respective type strains.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11475409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Dongia sedimenti</i> sp. nov., isolated from river sediment.","authors":"Xueting Jiang, Luyao Ruan, Ningning Wu, Dongmei Mao, Jian He, Shimei Wang, Jiangdong Jiang, Qirong Shen","doi":"10.1099/ijsem.0.006532","DOIUrl":"10.1099/ijsem.0.006532","url":null,"abstract":"<p><p>A Gram-stain-negative, non-spore-forming and strictly aerobic bacterial strain, designated R-7^T, was isolated from river sediment in Wuxi, Jiangsu, PR China. Cells (1.6-3.8 µm long and 0.6-0.8 µm wide) were slightly curved to straight rods and motile by means of a polar flagellum. The strain grew optimally on Reasoner's 2A medium at 30 °C, pH 7.0 and with 1.0% (w/v) NaCl. Strain R-7^T exhibited closest 16S rRNA gene sequence similarities to <i>Dongia mobilis</i> CGMCC 1.7660^T (95.4%), <i>D. rigui</i> 04SU4-P^T (94.6%) and <i>D. soli</i> D78^T (93.8%). The phylogenetic trees based on genomic and 16S rRNA gene sequences showed that strain R-7^T was clustered in the genus <i>Dongia</i>. The obtained average nucleotide identity and digital DNA-DNA hybridization values between R-7^T and the three type strains of the genus <i>Dongia</i> were 73.4, 72.8 and 72.4% and 19.5, 19.0 and 18.7%, respectively. The major respiratory quinone was Q-10. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, two unidentified aminophospholipids and nine unidentified polar lipids. The major cellular fatty acids (>5% of the total) were cyclo-C_19 : 0 <i> ω</i>8<i>c</i>, C_16 : 0 and C_16 : 0 2-OH. The DNA G+C content was 65.5 mol%. On the basis of the evidence presented in this study, strain R-7^T represents a novel species of the genus <i>Dongia</i>, for which the name <i>Dongia sedimenti</i> sp. nov. is proposed, with strain R-7^T (=KCTC 8082^T=MCCC 1K08805^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudohoeflea coraliihabitans</i> sp. nov., a poly-<i>β</i>-hydroxybutyrate-producing, halotolerant bacterium isolated from coral sediment in the Dapeng peninsula (Guangdong, China).","authors":"Sheng-Yang Yu, Xiao-Juan Lu, Meng-Jun Zhang, Sheng-Ji Luan, Yi Huang, Wei-Shuang Zheng","doi":"10.1099/ijsem.0.006453","DOIUrl":"https://doi.org/10.1099/ijsem.0.006453","url":null,"abstract":"<p><p>A Gram-stain-negative, strictly aerobic, motile, flagellated, rod-shaped, halotolerant, and poly-<i>β</i>-hydroxyalkanoate-producing bacterium, designated DP4N28-3^T, was isolated from offshore sediment surrounding hard coral in the Dapeng peninsula (Guangdong, PR China). Growth occurred at 15-35 °C (optimal at 30 °C), pH 6.0-9.5 (optimal at 6.0-7.0), and 0.0-30.0 % NaCl concentration (w/v, optimal at 0.0-2.0 %), showing halotolerance. Phylogeny based on 16S rRNA gene sequences, five housekeeping genes, and genome sequences identified <i>Pseudohoeflea suaedae</i> DSM 23348^T (98.1 %, 16S rRNA gene sequence similarity) as the most related species to strain DP4N28-3^T. Average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values between strain DP4N28-3^T and <i>P. suaedae</i> DSM 23348^T were all below the threshold of species demarcation. Major phenotypic differences were the flagella type and the limited sources of single carbon utilization by strain DP4N28-3^T, which only included acetic acid, acetoacetic acid, d-glucuronic acid, and glucuronamide. Strain DP4N28-3^T harboured the class I poly-<i>β</i>-hydroxyalkanoate synthase gene (<i>phaC</i>) and produced poly-<i>β</i>-hydroxybutyrate. The fatty acids were summed feature 8 (C_18 : 1 <i> ω</i>6<i>c</i> and/or C_18 : 1 <i> ω</i>7<i>c</i>, 49.4 %) and C_16 : 0 (13.4 %). The major cellular polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, and sulfoquinovosyl diacylglycerol. The respiratory quinone was Q-10. The results of the phylogenetic, genomic, phenotypic, and chemotaxonomic analysis indicated that the isolated strain represents the type strain of a novel species. Based on these results, strain DP4N28-3^T (=MCCC 1K05639^T=KCTC 82803^T) is proposed as the type strain of the novel species <i>Pseudohoeflea coraliihabitans</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of gut symbionts from wild-caught <i>Drosophila</i> and other Diptera: description of <i>Utexia brackfieldae</i> gen. nov., sp. nov., <i>Orbus sturtevantii</i> sp. nov., <i>Orbus wheelerorum</i> sp. nov, and <i>Orbus mooreae</i> sp. nov.","authors":"Laila E Phillips, Kathleen L Sotelo, Nancy A Moran","doi":"10.1099/ijsem.0.006516","DOIUrl":"https://doi.org/10.1099/ijsem.0.006516","url":null,"abstract":"<p><p>Non-culture based surveys show that the bacterial family <i>Orbaceae</i> is widespread in guts of insects, including wild <i>Drosophila</i>. Relatively few isolates have been described, and none has been described from <i>Drosophila</i>. We present the isolation and characterization of five strains of <i>Orbaceae</i> from wild-caught flies of the genera <i>Drosophila</i> (Diptera: Drosophilidae) and <i>Neogriphoneura</i> (Diptera: Lauxaniidae). Cells are generally rod-shaped, mesophilic, and measure 0.8-2.0 µm long by 0.3-0.5 µm wide. Optimal growth was observed under ambient atmosphere. Reconstruction of phylogenies from the 16S rRNA gene and from single-copy orthologs verify placement of these strains within <i>Orbaceae</i>. Cells exhibited similar fatty acid profiles to those of other <i>Orbaceae</i>. Strain lpD01^T shared 74% average nucleotide identity (ANI) with its closest relatives <i>Ca.</i> Schmidhempelia bombi Bimp and <i>Zophobihabitans entericus</i> IPMB12^T. Results from multiple genome-wide similarity comparisons indicate lpD01^T should be classified as a novel species within a novel genus. The major respiratory quinone for lpD01^T is ubiquinone Q-8. lpD02^T, lpD03, lpD04^T, and BiB^T are more closely related to <i>Orbus hercynius</i> CN3^T (76, 77, 76, and 77% ANI, respectively) than to other described <i>Orbaceae</i>. Genomic and phylogenetic analyses suggest that lpD03 and lpD04^T belong to the same species and that lpD02^T, lpD03/lpD04^T, and BiB^T are each novel species of the genus <i>Orbus</i>. The proposed names of these strains are <i>Utexia brackfieldae</i> gen. nov., sp. nov. (type strain lpD01^T =NCIMB 15517^T =ATCC TSD-399^T), <i>Orbus sturtevantii</i> sp. nov (type strain lpD02^T =NCIMB 15518^T =ATCC TSD-400^T), <i>Orbus wheelerorum</i> sp. nov. (type strain lpD04^T =NCIMB 15520^T =ATCC TSD-401^T), and <i>Orbus mooreae</i> sp. nov (type strain BiB^T=NCIMB 15516^T =ATCC TSD-402^T). The isolation and characterization of these strains expands the repertoire of culturable bacteria naturally associated with insects, including the model organism <i>D. melanogaster</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nocardiopsis codii</i> sp. nov., and <i>Rhodococcus chondri</i> sp. nov., two novel actinomycetal species isolated from macroalgae collected in the northern Portuguese coast.","authors":"Mariana Girão, Zoé Lequint, Adriana Rego, Isabel Costa, Diogo Neves Proença, Paula V Morais, Maria F Carvalho","doi":"10.1099/ijsem.0.006483","DOIUrl":"10.1099/ijsem.0.006483","url":null,"abstract":"<p><p>Two novel actinomycetal strains, designated CC-R113^T and CC-R104^T, were isolated from the tissues of two macroalgae collected on the northern Portuguese coast. Phylogenetic analyses based on the 16S rRNA gene showed that strain CT-R113^T belongs to the genus <i>Nocardiopsis</i>, being closely related to <i>Nocardiopsis umidischolae</i> 66/93^T and <i>Nocardiopsis tropica</i> VKM Ac-1457^T, with 98.65 and 98.39 % sequence similarity, respectively. The clade formed between the three type strains was confirmed by phylogenomic analysis. The genome of strain CT-R113^T was 7.27 Mb in size with a G+C content of 71.3 mol %, with average nucleotide identity (ANI) values of 89.59 and 90.14 % with strains 66/93^T and VKM Ac-1457^T, respectively. The major cellular fatty acids were identified as C_18 : 1 <i> ω</i>9<i>c</i>, iso-C_16 : 0 and anteiso-C_17 : 0. Menaquinone 10 (MK-10) was the major respiratory quinone. Comparative analysis of 16S rRNA gene sequences showed that strain CC-R104^T belongs to the genus <i>Rhodococcus</i> and is most closely related to <i>Rhodococcus pyridinivorans</i> DSM 44555^T, with 98.24 % sequence similarity. However, phylogenomic analysis revealed that strain CC-R104^T establishes a clade with <i>Rhodococcus artemisae</i> DSM 45380^T, being more distant from <i>Rhodococcus pyridinivorans</i> DSM 44555^T. The genome of strain CC-R104^T was 5.34 Mb in size with a G+C content of 67.01 mol%. The ANI value between strains CC-R104^T and DSM 45380^T was 81.2 % and between strains CC-R104^T and DSM 44555^T was 81.5 %. The major cellular fatty acids were identified as C_18 : 1 <i> ω</i>9<i>c</i>, C_16 : 0 and summed feature 3. Menaquinone 8 (MK-8) was the only respiratory quinone. For both CC-R113^T and CC-R104^T, optimum growth was observed at pH 7.0, 28 °C and 0-5 % NaCl and whole-cell hydrolysates contained <i>meso</i>-diaminopimelic acid as the cell-wall diamino acid. On the basis of phenotypic, molecular and chemotaxonomic characteristics, strains CT-R113^T and CC-R104^T are considered to represent novel species, for which the names <i>Nocardiopsis codii</i> sp. nov. (type strain CT-R113^T=LMG33234^T=UCCCB172^T) and <i>Rhodococcus chondri</i> sp. nov. (type strain CC-R104^T=LMG33233^T=UCCCB171^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11475406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}