Aurantivibrio plasticivorans gen. nov., sp. nov. and Aurantivibrio infirmus sp. nov., biodegradable plastic-degrading bacteria belonging to the family Cellvibrionaceae, isolated from the coast of Japan.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006920

Takamasa Miura, Rieko Kasaishi, Kohei Hidaka, Mamiko Shimamura, Moriyuki Hamada, Tomoyo Miyakawa, Yoko Kusuya, Yoshiyuki Ishitani, Shun'ichi Ishii, Yoko Furuno, Risa Yokoyama, Dai-Ichiro Kato, Asuka Arimoto, Kazuya Fukuda, Tatsuya Ueki, Kunifumi Tagawa, Yoshihito Uchino, Kei Kamino

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Abstract

Two Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterial strains, designated 4NK60-0013^T and 4NH20-0068^T, were isolated from biodegradable plastic films immersed in the coastal waters of Japan. These strains showed the ability to degrade biodegradable plastics, poly(butylene succinate-co-adipate) (PBSA) and poly(ε-caprolactone). Phylogenetic analyses based on the 16S rRNA gene sequences revealed that strains 4NK60-0013^T and 4NH20-0068^T belong to the family Cellvibrionaceae in the order Cellvibrionales and are closely related to members of the genus Gilvimarinus. The species most closely related to strains 4NK60-0013^T and 4NH20-0068^T were Gilvimarinus agarilyticus M5c^T and Gilvimarinus polysaccharolyticus YN3^T, with 16S rRNA gene sequence similarities of 94.4 and 93.3%, respectively. The sequence similarity between the two novel strains was 94.8%, indicating that they represented separate species. The genome length and G+C content of the genome sequence of strain 4NK60-0013^T were 4,354,061 bp and 46.9 mol%, respectively, whereas those of strain 4NH20-0068^T were 4,154,637 bp and 43.3 mol%, respectively. The average nucleotide identity values between the strains were <72.0%, similar to the values between the strains and type strains of species from the genera Cellvibrio, Gilvimarinus and Marinimicrobium. Based on phenotypic, cellular fatty acid and phylogenetic characteristics, the two isolates represent novel species within a new genus of the family Cellvibrionaceae, for which the names Aurantivibrio plasticivorans gen. nov., sp. nov. (type species) and Aurantivibrio infirmus sp. nov. have been proposed. Additionally, these strains and other bacteria belonging to the family Cellvibrionaceae have been shown to biodegrade PBSA, a biodegradable plastic. The type strains of A. plasticivorans and A. infirmus are 4NK60-0013^T (=NBRC 116180^T=TBRC 19146^T) and 4NH20-0068^T (=NBRC 116181^T=TBRC 19147^T), respectively.

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可塑金弧菌（Aurantivibrio plasticcivorans gen. nov., sp. nov.）和弱金弧菌（Aurantivibrio infirus sp. nov.）是一种生物可降解的塑料降解细菌，属于细胞弧菌科，分离自日本海岸。

从浸泡在日本沿海水域的可生物降解塑料薄膜中分离到两株革兰氏染色阴性、需氧、不形成孢子的棒状菌株，编号为4NK60-0013T和4NH20-0068T。这些菌株表现出降解生物可降解塑料、聚丁二酸-共己二酸丁二烯（PBSA）和聚ε-己内酯的能力。基于16S rRNA基因序列的系统发育分析表明，菌株4NK60-0013T和4NH20-0068T属于细胞弧菌目细胞弧菌科，与Gilvimarinus属成员关系密切。与菌株4NK60-0013T和4NH20-0068T亲缘关系最密切的种是Gilvimarinus agarilyticus M5cT和Gilvimarinus多糖olyticus YN3T，其16S rRNA基因序列相似性分别为94.4和93.3%。两个新菌株序列相似性为94.8%，表明它们属于不同的物种。菌株4NK60-0013T的基因组长度和G+C含量分别为4,354,061 bp和46.9 mol%，而菌株4NH20-0068T的基因组长度和G+C含量分别为4,154,637 bp和43.3 mol%。菌株间核苷酸的平均同一性值分别为Cellvibrio、Gilvimarinus和marinimicroum。根据表型、细胞脂肪酸和系统发育特征，这两株分离物代表了细胞弧菌科新属的新种，并被命名为可塑金弧菌（Aurantivibrio plasticcivorans gen. nov., sp. nov.）（模式种）和弱金弧菌sp. nov.。此外，这些菌株和其他属于细胞弧菌科的细菌已经被证明可以生物降解PBSA，一种可生物降解的塑料。塑形拟蚊和弱体拟蚊型菌株分别为4NK60-0013T （=NBRC 116180T=TBRC 19146T）和4NH20-0068T （=NBRC 116181T=TBRC 19147T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.