International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Pseudoalteromonas pernae</i> sp. nov., a novel bacterium with antimicrobial potential isolated from the intestinal tract of mussel genus <i>Perna</i> (Bivalvia: Mytilidae).","authors":"Suyun Fang, Xingyue Lin, Jun Min, Yan Li, Kim Lee Chang, Xiaoke Hu","doi":"10.1099/ijsem.0.006864","DOIUrl":"https://doi.org/10.1099/ijsem.0.006864","url":null,"abstract":"<p><p>The two bacterial strains, designated YIC-656^T and YIC-468, were isolated from the intestinal tract of <i>Perna</i> mussels (Bivalvia: Mytilidae) in China. Both strains were Gram-negative, aerobic, rod-shaped and motile, with a single polar flagellum. Strain YIC-656^T exhibited the highest 16S rRNA gene sequence similarity (98.4%) to strain <i>Pseudoalteromonas ruthenica</i> KMM300^T, followed by <i>Pseudoalteromonas caenipelagi</i> JBTF-M23^T and <i>Pseudoalteromonas xishaensis</i> E418^T, with sequence similarities of 98.3% and 98.2%, respectively. The 16S rRNA gene sequence similarity between strains YIC-656^T and YIC-468 was 99.9%, and their DNA G+C contents were both 45.3%. By genomic analyses, the average nucleotide identity and digital DNA-DNA hybridization values of strain YIC-656^T with any other related species were below 74.3% and 21.8%, while the indices between YIC-656^T and YIC-468 were 98.8% and 91.3%, respectively. These results indicate that strains YIC-656^T and YIC-468 belong to the same species and are distinct from other species within the genus <i>Pseudoalteromonas</i>. Phenotypic and chemotaxonomic tests further validated their differentiation from the closest relatives. The fatty acid profiles of both strains mainly (>7.0%) consisted of C_16:0, summed feature 3 (comprising C_16:1 <i> </i>ω<i>7c</i> and/or C_16:1 <i> </i>ω<i>6c</i>), summed feature 8 (comprising C_18:1 <i> </i>ω<i>7c</i> and/or C_18:1 <i> ω</i>6<i>c</i>) and C_18:0. The predominant respiratory quinone was Q-8. The polar lipids included phosphatidylethanolamine and unidentified lipids. Based on these phenotypic and genotypic results, strains YIC-656^T and YIC-468 represent a new species of <i>Pseudoalteromonas</i>, for which we propose <i>Pseudoalteromonas pernae</i> sp. nov. The type strain is YIC-656^T (=MCCC 1K08806^T=KCTC 8211^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Curtobacterium phyllosphaerae</i> sp. nov. and <i>Curtobacterium guangdongense</i> sp. nov., isolated from the citrus phyllosphere.","authors":"Songzhen Yang, Jiali Li, Xiang Rong, Qingying Chen, Xueying Lu, Jun Zhang, Ming-Rong Deng, Honghui Zhu, Guang-Da Feng","doi":"10.1099/ijsem.0.006870","DOIUrl":"https://doi.org/10.1099/ijsem.0.006870","url":null,"abstract":"<p><p>Two yellow-pigmented, Gram-stain-positive and aerobic bacterial strains, designated RRHDQ10^T and RRHDQ66^T, were isolated from citrus leaves collected from a well-known production area located in Renhua County of Guangdong Province, P. R. China. The 16S rRNA gene comparison showed that strain RRHDQ10^T shared the highest similarity to <i>Curtobacterium ammoniigenes</i> NBRC 101786^T (98.32%) and <i>Curtobacterium albidum</i> DSM 20512^T (98.32%), and strain RRHDQ66^T shared that to <i>Curtobacterium flaccumfaciens</i> JCM 9670^T (99.86%) and <i>Curtobacterium allii</i> 20TX0166^T (99.86%). Phylogenetic analyses based on the 16S rRNA gene and genome sequences showed that they belonged to the genus <i>Curtobacterium</i>, among which strain RRHDQ10^T was most closely related to <i>C. ammoniigenes</i> NBRC 101786^T, and strain RRHDQ66^T showed a close relationship with <i>C. flaccumfaciens</i> JCM 9670^T and <i>Curtobacterium herbarum</i> NBRC 103064^T. The genome-derived average nucleotide identity between the two strains and their closely related type strains was below 82.06% and 85.55%, respectively, and the corresponding digital DNA-DNA hybridization values were below 22.3% and 26.2%, respectively. Anteiso-C_15:0 and iso-C_16: 0 were their major fatty acids. Moreover, summed feature 8 (C_18:1 <i> ω</i>7<i>c</i> and/or C_18:1 <i> ω6</i>c) and anteiso-C_17 : 0 served as major components in strains RRHDQ10^T and RRHDQ66^T, respectively. They took menaquinone-9 (MK-9) as the predominant respiratory quinone and ornithine as the principal cell-wall diamino acid. Their major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and three unidentified glycolipids. The genomic DNA G+C contents of strains RRHDQ10^T and RRHDQ66^T were 71.2 and 70.5 mol%, respectively. Based on phenotypic, genotypic and chemotaxonomic analyses, they should be considered as two novel species of the genus <i>Curtobacterium</i>, for which the names <i>Curtobacterium phyllosphaerae</i> sp. nov. and <i>Curtobacterium guangdongense</i> sp. nov. are proposed, with RRHDQ10^T (=GDMCC 1.2671^T=JCM 34831^T) and RRHDQ66^T (=GDMCC 1.2666^T= JCM 34827^T) as the type strains, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Brachymonas wangyanguii</i> sp. nov., isolated from the coal samples in Shanxi Province of China.","authors":"Fangyu Gao, Mei Zhang, Qiang Yang, Lijun Zhao, Junyao Xie, Caixin Yang, Xiaojuan Du, Zhiming Kang, Ji Pu, Han Zheng, Jianguo Xu, Kui Dong","doi":"10.1099/ijsem.0.006837","DOIUrl":"10.1099/ijsem.0.006837","url":null,"abstract":"<p><p>Two Gram-negative, oxidase-positive, catalase-positive, non-spore-forming, motile, short-rod strains (G13^T and G34) were isolated from <i>in situ</i> coal block samples. Phylogenetic analyses based on 16S rRNA genes and 718 core genes indicated that both strains belonged to the genus <i>Brachymonas</i>, with the closest relative being <i>Brachymonas chironomi</i> AIMA4^T as their closest relative. Average nucleotide identity and digital DNA-DNA hybridization values were 79.4-89.27% and 23.6-37.0% below the species-level thresholds. Further chemotaxonomic analysis revealed that both strains possessed C_16:1 <i>ω</i>7<i>c</i> and/or C_16:1 <i>ω</i>6<i>c</i>, C_18:1 <i>ω</i>7<i>c</i> and C_16:0 as major cellular fatty acids, with Q-8 as the predominant respiratory quinone. The polar lipid profile was dominated by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and phylogenetic evidence support the proposal that strain G13^T represents a novel species of the genus <i>Brachymonas</i>, with the name <i>Brachymonas wangyanguii</i> sp. nov. The type strain is G13^T (=GDMCC 1.4691^T=JCM 37746^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From cantaloupe to cattle: <i>Pseudomonas alabamensis</i> sp. nov. described from diseased cantaloupe (<i>Cucumis melo</i>) foliage and a bovine (<i>Bos taurus</i>) nasopharynx.","authors":"Kiersten R Fullem, Michelle P MacLellan, Fanny B Iriarte, Mousami Poudel, Sarah Capik, Keith Dedonder, James L Bono, Dayna M Harhay, Gregory P Harhay, Erica M Goss, Neha Potnis, Gerald V Minsavage, Jeffrey B Jones, Mathews L Paret","doi":"10.1099/ijsem.0.006848","DOIUrl":"10.1099/ijsem.0.006848","url":null,"abstract":"<p><p>In 2022, a fluorescent bacterial isolate, designated 22-AL-CL-001, was isolated from diseased cantaloupe (<i>Cucumis melo</i>) foliage collected in Alabama, USA, displaying marginal necrosis and general blighting. Whole-genome sequencing and subsequent comparisons to publicly available bacterial genomes identified <i>Pseudomonas</i> isolate USDA-ARS-USMARC-56711, isolated from a clinically healthy calf (<i>Bos taurus</i>) in Kansas, USA, as genetically similar to isolate 22-AL-CL-001. The two isolates were then characterized using phenotypic and genetic analyses, including fluorescence on King's medium B; LOPAT reactions; pathogenicity assays on cantaloupe, watermelon and squash plants; 16S rRNA gene sequence analysis; phylogeny based on multi-locus sequence analysis (MLSA) of four housekeeping genes; average nucleotide identity based on blast (ANIb); <i>in silico</i> DNA-DNA hybridization (isDDH) including comparison to the Type Genome Server and biochemical profiling using the Biolog Gen III microplate system. MLSA phylogeny placed both isolates into a single clade within the genus <i>Pseudomonas</i>, distant from all included reference strains. ANIb and isDDH analyses definitively identified the isolates as members of the same species (ANIb, 97.25; isDDH, 78.5%), though comparison scores to reference strains were all below the accepted thresholds for species determination. Biolog biochemical profiling, as well as MALDI-TOF mass spectrometry of isolate 22-AL-CL-001, was also unable to identify the isolate as a member of any existing bacterial species. Using a combination of genetic and phenotypic data, we conclude that the two isolates belong to a new species of <i>Pseudomonas</i>, for which we propose the name <i>Pseudomonas alabamensis</i>. The specific epithet, <i>alabamensis</i>, was chosen to represent the location where the type strain 22-AL-CL-001^T (NCPPB 4760^T=LMG 33363^T) was isolated, Alabama, USA. Taxonomic classification of the two isolates by the Genome Taxonomy Database revealed other previously sequenced bacterial strains that, according to ANI and isDDH results, also belong to <i>P. alabamensis</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification of changes in taxonomic opinion previously published outside the <i>IJSEM</i>. List of Changes in Taxonomic Opinion no. 42.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006793","DOIUrl":"10.1099/ijsem.0.006793","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12313057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 75, part 3 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006765","DOIUrl":"10.1099/ijsem.0.006765","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12214243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Erratum: Arcobacter iocasae</i> sp. nov., a bacterium isolated from the gill homogenate of the mussel <i>Gigantidas platifrons</i> in a cold seep.","authors":"Huan Zhang, Shi Jin, Hao Wang, Fei Li, Zhaoshan Zhong, Yang Guo, Jia Luo, Ziyun Ye, Chao Lian, Minxiao Wang, Chaolun Li","doi":"10.1099/ijsem.0.006854","DOIUrl":"https://doi.org/10.1099/ijsem.0.006854","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Macrococcus animalis</i> sp. nov. and <i>Macrococcus equi</i> sp. nov., isolated from different animals' origins.","authors":"Chahrazed Belhout, Fangkun Wang, Alexandra Rossano, Alexandra Collaud, Javier E Fernandez, Emma Marchionatti, Jennifer Eleonora Keller, Gudrun Overesch, Sabine Kaessmeyer, Sybille Schwendener, Vincent Perreten","doi":"10.1099/ijsem.0.006861","DOIUrl":"https://doi.org/10.1099/ijsem.0.006861","url":null,"abstract":"<p><p>Nine Gram-positive, non-motile, facultatively anaerobic cocci, designated EM39Eᵀ, JEK85, 18KM676, 21M1142, 18KM445^T, 18KM444, 18KM245, 18KM583 and 21KM1573, were isolated from diverse animal material, including horse and pig skin, bovine mastitis milk and feline urine from a urinary tract infection. Phylogenomic analysis based on amino-acid alignment obtained from translated whole-genome sequences; digital DNA-DNA hybridization (dDDH); 16S rRNA, <i>dnaJ</i>, <i>hsp60</i>, <i>rpoB</i> and <i>sodA</i> gene comparison; and MALDI-TOF MS spectral profiles placed the strains within the <i>Macrococcus caseolyticus</i> clade. They were closely related to <i>Macrococcus bohemicus</i> CCM 7100ᵀ, <i>Macrococcus epidermidis</i> CCM 7099ᵀ, <i>Macrococcus goetzii</i> CCM 4927ᵀ and <i>Macrococcus capreoli</i> DSM 113939^T. The novel species exhibited 22.30% dDDH and 79.49% average nucleotide identity (ANI) to each other, <24% dDDH and <82% ANI to their closest relatives, values below established species delineation thresholds, confirming their classification as two distinct novel species. Chemotaxonomic analysis identified, in both species, diphosphatidylglycerol, phosphatidylglycerol, aminolipid, glycolipids and phosphoglycolipids, as the major polar lipids. Fatty acid profiling revealed C_14 : 0, C_16 : 1 ω11c, C_16 : 0 and C_18 : 1 ω13c as predominant components, with antesio-C_15 : 0 in one species. The peptidoglycan type was A3α l-Lys-Gly2. The novel species can be distinguished from their closest relatives by their ability to grow in 12.5% NaCl. EM39Eᵀ, JEK85, 18KM676 and 21M1142 can be distinguished by positive reaction to d-ribose, and 18KM445^T, 18KM444, 18KM245, 18KM583 and 21KM1573 by the absence of <i>α</i>-glucosidase activity and acid production from methyl-<i>β</i>-d-glucopyranoside and maltose. The two novel species can be differentiated from each other by d-ribose metabolism and methyl-<i>β</i>-d-glucopyranoside hydrolysis. 18KM445^T, 18KM444, 18KM245, 18KM583 and 21KM1573 formed a distinct cluster of strains, designated <i>Macrococcus equi</i> sp. nov., with strain 18KM445ᵀ (=DSM 118744ᵀ; =CCOS 2124ᵀ; =CCM 9438ᵀ) as the proposed type strain. The second cluster, containing strains EM39Eᵀ, JEK85, 18KM676 and 21M1142, was designated <i>Macrococcus animalis</i> sp. nov., with strain EM39Eᵀ (=DSM 118743ᵀ; =CCOS 2125ᵀ; =CCM 9439ᵀ) as the proposed type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of [<i>Pasteurella</i>] <i>langaaensis</i> as <i>Alitibacter langaaensis</i> gen. nov., comb. nov.","authors":"Magne Bisgaard, Henrik Christensen","doi":"10.1099/ijsem.0.006867","DOIUrl":"https://doi.org/10.1099/ijsem.0.006867","url":null,"abstract":"<p><p>[<i>Pasteurella</i>] <i>langaaensis</i> has been found to be polyphyletic in relation to the type species <i>Pasteurella multocida</i>, as endicated by 16S rRNA gene sequence comparisons and a number of other comparisons (enclosing the genus name in brackets). 16S rRNA gene sequence-based phylogeny confirmed that [<i>P</i>.] <i>langaaensis</i> is unrelated to other members of <i>Pasteurellaceae</i> at the genus level and may be a candidate for a new genus with 95% 16S rRNA gene sequence identity to the closest related sequence belonging to the type strain of [<i>Actinobacillus</i>] <i>rossii</i>. The highest digital DNA-DNA hybridization value predicted from the whole-genomic sequence of the type strain of [<i>P</i>.] <i>langaaensis</i> was to the type strain of <i>Acinobacillus suis</i> with 26%, confirming a separate taxonomic status at the species level. The phylogenetic comparison of concatenated conserved protein sequences determined in a previous study showed a unique position of the taxon investigated, which qualified for the status of a new genus since the highest conserved protein sequence identity was found to [<i>A</i>.] <i>rossii</i> with 86%. A new genus with one species, <i>Alitibacter langaaensis</i> gen. nov., comb. nov., is proposed. The genus can be separated from other genera in the family <i>Pasteurellaceae</i> by from 1 up to 12 phenotypic characters, and it is monotypic from other genera with respect to analysis of 16S rRNA gene sequences as well as of predicted core genomic proteins. The type strain of <i>Alitibacter langaaensis</i> is F73^T (=NCTC 11411^T=CCUG 15566^T=DSM 22999^T=CPI 102678^T=ATCC 43328^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nioella halotolerans</i> sp. nov., a novel member of the family <i>Roseobacteraceae</i>, isolated from a saline lake.","authors":"Shuai-Bo Han, Xin-Jun Hou, Yu Li, Xiao-Long He, Rui-Jun Wang, Xu-Fen Zhu, Min Wu","doi":"10.1099/ijsem.0.006858","DOIUrl":"10.1099/ijsem.0.006858","url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic bacterium, designated W635^T, was isolated from saline lake water collected from Lake Ayaqqum in Xinjiang province, China. Cells were catalase and oxidase positive, rod-shaped and non-motile. Growth occurred at 20-37 °C (optimum 28-30 °C), pH 7.0-9.5 (optimum 7.5-8.0) and with 0.5-16.0% NaCl (optimum 2.0-4.0%, w/v). Chemotaxonomic analysis revealed that the sole respiratory quinone was ubiquinone-10. The major fatty acids included C_16:0, C_19:0 cyclo <i>ω</i>8<i>c</i>, C_18:1 <i> ω</i>7<i>c</i> 11-methyl and summed feature 8 (C_18:1 <i> ω</i>7<i>c</i> and/or C_18:1 <i> ω</i>6<i>c</i>). The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one uncharacterized phospholipid and four uncharacterized lipids. Sequence similarity analysis of the 16S rRNA gene revealed that strain W635^T presented the highest similarity to <i>Roseovarius nitratireducens</i> TFZ^T (95.47%), followed by members of the genus <i>Nioella</i> (95.11-95.25%). Phylogenetic analysis based on multilocus sequence analysis and whole-genome sequencing indicated that strain W635^T is most closely related to the genus <i>Nioella</i>. The genomic DNA G+C content of the strain W635^T was 65.8 mol%. The average amino acid identity values between strain W635^T and <i>Nioella nitratireducens</i> SSW136^T, <i>Nioella sediminis</i> JS7-11^T, <i>Nioella aestuarii</i> JCM30752^T, and <i>Nioella ostreopsis</i> Z7-4^T were 79.02%, 80.04%, 79.77% and 80.05%, respectively. The average nucleotide identity and <i>in silico</i> DNA-DNA hybridization values between strain W635^T and these strains were 78.37-79.78% and 19.8-20.80%, respectively. Based on phenotypic, chemotaxonomic, genotypic and genomic characteristics, strain W635^T represents a novel species of the genus <i>Nioella</i>, for which the name <i>Nioella halotolerans</i> is proposed. The type strain is W635^T (=KCTC 62429^T =MCCC 1K03491^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144730899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}