International journal of systematic and evolutionary microbiology最新文献

{"title":"Reclassification of atypical <i>Moraxella catarrhalis</i> ATCC 23246 as <i>Moraxella veridica</i> sp. nov.","authors":"Scott V Nguyen, Vicktor H Escobar, Shahin S Ali, Nikhita P Puthuveetil, Joseph R Petrone, Jade L Kirkland, Kaitlyn Gaffney, Corina L Tabron, Noah Wax, James Duncan, Stephen King, Robert Marlow, Amy L Reese, David A Yarmosh, Hannah H McConnell, Ana S Fernandes, John Bagnoli, Briana Benton, Jonathan L Jacobs","doi":"10.1099/ijsem.0.006797","DOIUrl":"10.1099/ijsem.0.006797","url":null,"abstract":"<p><p>Whole-genome sequencing of cultures at the American Type Culture Collection (ATCC^®) is ongoing, with reference-quality genome sequences for our microbial strains added to the ATCC^® Genome Portal on a quarterly basis. Following genome assembly, authentication and taxonomy verification are needed for taxonomic updates based on the circumscription of genomic metrics for a species. <i>Moraxella</i> sp. ATCC 23246^T was originally identified as an atypical <i>Moraxella catarrhalis</i>; however, an analysis of the complete and closed genome of this strain indicates that it represents a novel species within the <i>Moraxella</i> genus. We propose the name of <i>Moraxella veridica</i> sp. nov. for this long-mischaracterized strain as whole-genome sequencing was used to uncover the truth of this strain's identity. The type strain is ATCC 23246^T (=NCTC 4103^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Comamonas trifloxystrobinivorans</i> sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.","authors":"Qian Zhu, Kangning Wei, Kaihua Pan, Qian Li, Weihao Zhu, Mingliang Zhang, Qing Hong","doi":"10.1099/ijsem.0.006796","DOIUrl":"https://doi.org/10.1099/ijsem.0.006796","url":null,"abstract":"<p><p>A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3^T, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3^T could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3^T; it exhibited low similarity (<i><</i>31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3^T was most closely related to strain <i>Comamonas odontotermitis</i> Dant 3-8^T. The 16S rRNA gene similarity between strain J-3^T and <i>C. odontotermitis</i> Dant 3-8^T was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3^T and strain <i>C. odontotermitis</i> Dant 3-8^T were 81.7% and 25.4%, respectively. The major fatty acids were C_16:0, summed feature 3 (C_16:1 <i> ω7</i>c and/or C_16:1 <i> ω6</i>c) and summed feature 8 (C_18 : 1 <i> ω7</i>c and/or C_18 : 1 <i> ω6</i>c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3^T was considered to represent a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas trifloxystrobinivorans</i> sp. nov. is proposed with strain J-3^T (=KCTC 8781^T=MCCC 1K09559^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Labrys sedimenti</i> sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of <i>Labrys portucalensis</i> as a later heterotypic synonym of <i>Labrys neptuniae</i>.","authors":"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt","doi":"10.1099/ijsem.0.006778","DOIUrl":"https://doi.org/10.1099/ijsem.0.006778","url":null,"abstract":"<p><p>A Gram-stain-negative strain, designated as Zidic-5^T, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5^T belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578^T (99.13%), followed by <i>Labrys portucalensis</i> F11^T (99.06%), <i>Labrys methylaminiphilus</i> JLW10^T (98.58%) and <i>Labrys okinawensis</i> DSM 18385^T (98.32%). The draft genome sequence of strain Zidic-5^T is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5^T and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5^T is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5^T were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5^T were C_18 : 1 ω7c, C_16 : 0 and C_19 : 0 cyclo ω7c. The major ubiquinone of strain Zidic-5^T was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5^T represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5^T (=LMG 33565^T=NCAIM B.02686^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Vreelandella arctica</i> sp. nov. and <i>Vreelandella indica</i> sp. nov., isolated from the Arctic Ocean and the Southwest Indian Ocean sediment.","authors":"Yuwei Weng, Jingyu Xia, Xuying Zhu, Wanpeng Wang","doi":"10.1099/ijsem.0.006791","DOIUrl":"10.1099/ijsem.0.006791","url":null,"abstract":"<p><p>Four Gram-stain-negative bacterial strains which can metabolize alkanes and polycyclic aromatic hydrocarbons were isolated. Strains R06ZXJ2^T and R09ZXJ6 were isolated from sediments collected in the Arctic Ocean. Strains DY715-9^T and DY716-13-1 were isolated from surface sediment samples collected by the deep-sea TV grab in the southwestern Indian Ocean. Phylogenomic analyses based on 16S rRNA gene sequences, average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) revealed that the strains formed two distinct lineages within the genus <i>Vreelandella</i>. Their closest relative was <i>Vreelandella maris</i> QX-1^T, with ANI values of 94.35% (R06ZXJ2^T) and 95.54% (DY715-9^T) and dDDH values of 57.00% and 61.80%, respectively. The pairwise ANI (95.47%) and dDDH (60.70%) between the two novel strains further supported their status as separate species. Phenotypic and chemotaxonomic analyses distinguished them from known <i>Vreelandella</i> species through differences in colony morphology, hydrocarbon degradation profiles and polar lipid compositions. The strains grew optimally at 28 °C and pH 7.0 and can degrade alkanes and polycyclic aromatic hydrocarbons. Based on these results, we propose two novel species: <i>Vreelandella arctica</i> sp. nov. (type strain R06ZXJ2^T=MCCC 1A08467^T=KCTC 92631^T) and <i>Vreelandella indica</i> sp. nov. (type strain DY715-9^T=MCCC 1A12469^T=KCTC 92628^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptococcus hepaticus</i> sp. nov. isolated from the liver of domestic pigs (<i>Sus scrofa domesticus</i>).","authors":"Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore","doi":"10.1099/ijsem.0.006776","DOIUrl":"https://doi.org/10.1099/ijsem.0.006776","url":null,"abstract":"<p><p>A Gram-positive coccus-shaped bacterium, which could not be identified by classical determinative bacteriology approaches, was isolated from the liver of two pigs in the United Kingdom. Initial testing based on cellular morphology and biochemical characteristics tentatively assigned the isolates to the genus <i>Streptococcus</i> but did not match any previously described species. The analysis of the 16S rRNA sequence determined that the isolates were most closely related to <i>Streptococcus gallinaceus</i> (98.6% identity). Analysis of three further housekeeping genes frequently applied in streptococcal taxonomy, <i>groEL</i>, <i>sodA</i> and <i>rpoB</i>, and a comparison against available type strain sequences confirmed that the isolates were most similar to <i>S. gallinaceus</i> in all cases (84.3%, 86.9% and 90.2% identity, respectively). The comparison of the average nucleotide identity (ANI) and <i>in silico</i> DNA-DNA hybridization values demonstrated that the novel species was distinct from other streptococcal species. Pairwise ANI values revealed that the two studied strains shared a pairwise ANI of 99.25% but were clearly distinct from previously described <i>Streptococcus</i> species (ANI ≤81.1% - best match <i>S. gallinaceus</i>). The taxonomic analysis described confirmed that the two strains represent a novel <i>Streptococcus</i> species for which the name <i>Streptococcus hepaticus</i> sp. nov. is suggested, with strain 20-1249^T (=NCTC 15092^T=LMG 33498^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Imbroritus primus</i> gen. nov., sp. nov., a facultatively autotrophic bacterium from environmental water samples.","authors":"Charlotte Peeters, Stephanie Steyaert, Matan Shelomi, Anneleen D Wieme, Eliza Depoorter, Evelien De Canck, Kurt Houf, Peter Vandamme","doi":"10.1099/ijsem.0.006781","DOIUrl":"https://doi.org/10.1099/ijsem.0.006781","url":null,"abstract":"<p><p>A Gram-stain-negative coccobacillus, LMG 32992^T, was isolated from water that had collected in a tyre in Pingtung, Donggang Township, Taiwan. Upon preliminary 16S rRNA gene sequence analysis, it was most closely related to members of the genus <i>Ralstonia</i> (16S rRNA gene sequence similarities of 96.7-97.5%). The present study aimed to elucidate its taxonomic position and to propose a formal classification. To this end, the complete genome sequence was determined, and taxonomic, phylogenomic, metabolic and physiological analyses were performed. Comparative genomic analyses demonstrated that strain LMG 32992^T and another unclassified strain, <i>Burkholderiaceae</i> bacterium PBA, which was isolated earlier from textile wastewater in Malaysia, represented a single novel species within a novel genus of the family <i>Burkholderiaceae</i>. The G+C content of the LMG 32992^T genomic DNA was 63.77 mol%. Genomic analyses and growth tests demonstrated that LMG 32992^T had an asaccharolytic metabolism but that it was well-equipped to synthetize, if necessary autotrophically, and transform all required carbohydrates and that it used the Krebs and related cycles to generate reductive power for a heterotrophic energy metabolism. We propose the name <i>Imbroritus primus</i> gen. nov., sp. nov. with strain LMG 32992^T (=CIP 112179^T=BCRC 81361^T=A30B1^T) as the type strain, for this novel taxon.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Janthinobacterium aestuarii</i> sp. nov., a novel violacein-producing bacterium isolated from the Tidal Basin in Washington, D.C., USA.","authors":"Christina Pavloudi, Maximilian D Shlafstein, Yesmarie T De La Flor, Emma M Smith, Ana C Peñuela, Erin Papke, Lausanne Oliver, Kaitlynn Slattery, Guinevere Lissner, Lívia C Coelho, David K Oline, Patrick Videau, Wendy K Strangman, Blake Ushijima, Jimmy H Saw","doi":"10.1099/ijsem.0.006768","DOIUrl":"https://doi.org/10.1099/ijsem.0.006768","url":null,"abstract":"<p><p>Strain TB1-E2-13^T was isolated from water collected from the Tidal Basin in Washington, D.C., USA, due to the bright purple colour of its colonies, and was taxonomically evaluated with a polyphasic approach. Comparison of a partial 16S rRNA gene sequence found that strain TB1-E2-13^T was most similar to species in the <i>Janthinobacterium</i> genus. For more precise taxonomic inference, a phylogenomic analysis was conducted and indicated that strain TB1-E2-13^T was most closely related to <i>Janthinobacterium lividum</i>, '<i>Janthinobacterium kumbetense</i>', <i>Janthinobacterium rivuli</i> and <i>Janthinobacterium violaceinigrum</i>. Analyses of genomic indices found that pairwise comparisons between strain TB1-E2-13^T and other members of the <i>Janthinobacterium</i> genus returned values below the threshold of species novelty. Based on a polyphasic characterization and identifying differences in genomic and taxonomic data, strain TB1-E2-13^T represents a novel species, for which the name <i>Janthinobacterium aestuarii</i> sp. nov. is proposed. The type strain is TB1-E2-13^T (=ATCC TSD-339^T=JCM 36076^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12048719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected 16S rRNA heterogeneity in '<i>Acetobacterium dehalogenans</i>' and reclassification as <i>Acetobacterium malicum</i> subsp. <i>dehalogenans</i> subsp. nov.","authors":"Stefan Spring, Jacqueline Wolf, Sarah Kirstein, Cathrin Spröer, Boyke Bunk","doi":"10.1099/ijsem.0.006783","DOIUrl":"https://doi.org/10.1099/ijsem.0.006783","url":null,"abstract":"<p><p>Strain MC^T is a strictly anaerobic, homoacetogenic bacterium with the ability to utilize methyl chloride as the sole energy source. It was tentatively assigned to the genus <i>Acetobacterium</i> as '<i>Acetobacterium dehalogenans</i>'. Due to sequence ambiguities, it was not possible to determine the 16S rRNA gene sequence of this strain by direct sequencing of a PCR-amplified DNA segment. Whole-genome sequencing revealed significant heterogeneity amongst the five rRNA operons detected in this strain, with maximum sequence differences between the individual 16S rRNA genes exceeding 1.4%, compared to <0.8% in related species. Genome comparisons identified strain MC^T as most closely related to <i>Acetobacterium malicum</i> MuME1^T, with a digital DNA-DNA hybridization value of 71.9% and an average nucleotide identity score of 96.59%, indicating that the strains belong to the same species. Both strains share the ability to utilize malate, a key feature of <i>A. malicum</i>, but differ in the utilization of methanol and glucose. Chemotaxonomic analyses also revealed distinct fatty acid and polar lipid patterns. Based on these findings, we propose the classification of strain '<i>Acetobacterium dehalogenans</i>' MC^T (=DSM 11527^T=NBRC 117038^T) as <i>A. malicum</i> subsp. <i>dehalogenans</i> subsp. nov. This automatically establishes <i>A. malicum</i> subsp. <i>malicum</i> subsp. nov., with MuME1^T (=DSM 4132^T=ATCC 51201^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based reclassification of <i>Terribacillus goriensis</i> (Kim <i>et al</i>. 2007) Krishnamurthi and Chakrabarti 2009 as a later heterotypic synonym of <i>Terribacillus saccharophilus</i> An <i>et al</i>. 2007.","authors":"Theresa Hauser, Paul A Lawson, Durward L Bevis, Richard W McLaughlin","doi":"10.1099/ijsem.0.006794","DOIUrl":"https://doi.org/10.1099/ijsem.0.006794","url":null,"abstract":"<p><p>The present study used whole-genome data to clarify the taxonomic assignment of two closely related <i>Terribacillus</i> species. The genus <i>Terribacillus</i> includes four species with validly published names, <i>Terribacillus aidingensis, T. goriensis, T. halophilus</i> and <i>T. saccharophilus</i>. The type species <i>T. saccharophilus</i> 002-048^T (=IAM 15309^T=KCTC 13936^T) was isolated from the soil in Japan, while <i>T. goriensis</i> CL-GR16^T (=KCCM 42329^T=DSM 18252^T) was isolated from the coastal water off the east coast of Korea. Both strains have similar phenotypic and chemotaxonomic characteristics. Anteiso-C_{15 : 0} was the major fatty acid, followed by anteiso-C_17 : 0 and iso-C_15 : 0. There were similar major menaquinones (MK-7). As determined by whole-genome sequencing, the DNA G+C content of 42.53 and 42.66 mol% was found for <i>T. saccharophilus</i> and <i>T. goriensis</i>, respectively, and they were 99.86% identical in their 16S rRNA gene sequences (1,421 bp). Genomic comparisons showed the average nucleotide identity was 98.29% and 84.80% for digital DNA-DNA hybridization. These values exceeded the threshold values for bacterial species delineation. Using the Type (Strain) Genome Server, both species were identified as <i>T. saccharophilus</i>. Phylogenetic analysis based on both 16S rRNA gene sequences and whole-genome sequences showed that they form a separate cluster apart from members of other genera. Based on the combined evidence, <i>Terribacillus goriensis</i> Kim <i>et al</i>. (<i>Int J Syst Evol Microbiol</i> 2007;57:1554-1560), Krishnamurthi and Chakrabarti (<i>Int J Syst Evol Microbiol</i> 2008;58:2287-2291) is proposed to be a later heterotypic synonym of An <i>et al</i>. (<i>Int J Syst Evol Microbiol</i> 2007;57:51-55).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Listeria tempestatis</i> sp. nov. and <i>Listeria rocourtiae</i> subsp. <i>hofi</i> subsp. nov.","authors":"Phillip Brown, Alexandra Moura, Guillaume Valès, Nathalie Tessaud-Rita, Jefffrey Niedermeyer, Cameron Parsons, Alexandre Leclercq, Angela Harris, Ryan E Emanuel, Sophia Kathariou, Marc Lecuit","doi":"10.1099/ijsem.0.006774","DOIUrl":"https://doi.org/10.1099/ijsem.0.006774","url":null,"abstract":"<p><p>In September 2018, Hurricane Florence resulted in major flooding in North Carolina, USA. Efforts to isolate <i>Listeria monocytogenes</i> and other <i>Listeria</i> spp. from Hurricane Florence floodwaters repeatedly yielded non-haemolytic <i>Listeria</i>-like isolates that could not be readily assigned to known <i>Listeria</i> taxa. Whole-genome sequence analyses against the 28 currently known <i>Listeria</i> species confirmed that the isolates constitute two new taxa within the genus <i>Listeria</i>. Taxon I, with one isolate, showed the highest similarity to <i>Listeria goaensis,</i> with an average nucleotide identity blast of 85.3±4.4% and an <i>in silico</i> DNA-DNA hybridization (<i>is</i>DDH) of 32.4% (range: 30-35%), differing from the latter by its ability to reduce nitrate, ferment d-ribose and sucrose, and by its inability to produce catalase or ferment d-trehalose and d-lactose. Taxon II, represented by 11 isolates, showed the highest similarity to <i>Listeria rocourtiae,</i> with an average nucleotide identity blast of 92.64±3.8% and an <i>is</i>DDH of 49.9% (range: 47.3-52.5%), differing from the latter by its ability to ferment l-arabinose and its inability to ferment l-rhamnose, d-galactose, d-lactose and d-melibiose. The names <i>Listeria tempestatis</i> sp. nov. and <i>Listeria rocourtiae</i> subsp. <i>hofi</i> subsp. nov. are proposed for taxon I and II, respectively, with type strains CLIP 2022/01175^T (F6L-1A=CIP 112444^T = DSM 117029^T) and CLIP 2022/01000^T (F66L-1A=CIP 112443^T = DSM 117030^T), respectively. Both taxa lack known <i>Listeria</i> pathogenic islands, suggesting a lack of pathogenicity for humans.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}