International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Taphrina neopruni</i> sp. nov., a novel species associated with plum pockets on <i>Prunus salicina</i>.","authors":"Kyoung-Mo Koo, Jin-Woo Park, Min-Jun Ko, Gyo-Seon Shin, Ji-Hyun Park, Hyeon-Dong Shin","doi":"10.1099/ijsem.0.007264","DOIUrl":"https://doi.org/10.1099/ijsem.0.007264","url":null,"abstract":"<p><p>The ascomycete genus <i>Taphrina</i> comprises dimorphic plant-parasitic pathogens, several species of which cause plum pockets. Plum pocket pathogens on Japanese plum (<i>Prunus salicina</i>) were collected from seven localities in Korea, including Gimcheon (KCTC 37381^T/KACC 411123), Gumi, Okcheon (KCTC 37382/KACC 411126), Boeun, Jeonju, Wanju (KACC 410969) and Naju (KCTC 37383/KACC 411129). Based on a polyphasic approach combining morphological and physiological characterization with a molecular phylogenetic analysis involving the internal transcribed spacer (ITS) region, the D1/D2 domain of the 26S rRNA gene and the mitochondrial small subunit ribosomal RNA (<i>rns</i>) gene, these isolates were confirmed to represent a novel <i>Taphrina</i> species. Phylogenetically, the novel species was closely related to a clade comprising <i>Taphrina pruni</i> and <i>Taphrina mume</i>, which cause plum pocket disease and apricot leaf curl, respectively. Compared with the type strain of <i>T. pruni</i> (HA 1306ᵀ), the new Korean isolates exhibited low sequence similarity, with 95.79%, 98.95% and 98.77% identities in the ITS, D1/D2 domain and <i>rns</i> regions, respectively. Isolates infecting <i>P. salicina</i> formed an independent clade distinct from all known <i>Taphrina</i> species. The isolates also differed from <i>T. pruni</i> in ascospore size, budding rate within asci and cultural characteristics. Physiologically, the isolates differed from <i>T. pruni</i> HA 1306ᵀ in 7 of 41 carbon assimilation tests, 4 of 10 nitrogen assimilation tests, and growth at 25 ℃. To date, plum pocket pathogens on <i>P. salicina</i> have been attributed to <i>T. pruni</i>, <i>Taphrina communis</i> and <i>Taphrina deformans</i>; however, the present study shows that they represent a novel species. We propose the name <i>Taphrina neopruni</i> sp. nov. (MycoBank number MB 862651) and designate KCTC 37381^T (ex-type strain KACC 411123) as the holotype.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Buttiauxella cochleicola</i> sp. nov., a novel coliform bacterium with <i>β</i>-d-glucuronidase activity, isolated from gastropods and drinking water.","authors":"Michael Hügler, Carolin Leister, Vera Aschmann, Monika Bösl, Lydia Grabner, Luisa Kurcz, Sarah Kirstein, Jacqueline Wolf, Richard L Hahnke, Vera Thiel","doi":"10.1099/ijsem.0.007265","DOIUrl":"10.1099/ijsem.0.007265","url":null,"abstract":"<p><p>Four strains of coliform bacteria were isolated from drinking water (strains A2-C1_F and A2-C2_NF) and gastropod samples (strains W03-F01^T and S04-F03). 16S rRNA gene sequence analysis showed the highest similarities to members of the genus <i>Buttiauxella</i> (>98%), indicating that all strains are members of this genus. Phylogenomic analyses revealed that strains W03-F01^T, S04-F03 and A2-C1_F can be assigned to a putative new <i>Buttiauxella</i> species, while strain A2-C2_NF is a member of the species <i>Buttiauxella ferragutiae</i>. The strains W03-F01^T, S04-F03 and A2-C1_F show fluorescence in the Colilert system, indicating unusual <i>β</i>-d-glucuronidase activity, typically considered indicative for <i>Escherichia coli</i> (and thus faecal contamination) in water quality analysis. The draft genome of strain W03-F01^T has a size of 4.79 Mb and a G+C content of 50.0 mol%. With average nucleotide identity and digital DNA-DNA hybridization values of 96.2 and 68.8%, respectively, strain W03-F01^T was most closely related to <i>Buttiauxella massiliensis</i> DSM 110695^T. Additional extensive physiological, phenotypic and chemotaxonomic characterization of the strains and all reference strains confirmed the proposal of a novel species. Therefore, the strains W03-F01^T, S04-F03 and A2-C1_F represent a novel species for which the name <i>Buttiauxella cochleicola</i> sp. nov. is proposed. The type strain is W03-F01^T (=DSM 113920^T=CIP 112567^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148721856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 76, part 5 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.007223","DOIUrl":"https://doi.org/10.1099/ijsem.0.007223","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Staphylococcus xeri</i> sp. nov., a haemolytic bacterium isolated from the nasal cavity's discharge of a Cape ground squirrel (<i>Xerus inauris</i>).","authors":"Chahrazed Belhout, Andreas Thomann, Alexandra Rossano, Alexandra Collaud, Vincent Perreten","doi":"10.1099/ijsem.0.007253","DOIUrl":"https://doi.org/10.1099/ijsem.0.007253","url":null,"abstract":"<p><p>Strain 17KM0847^T, isolated from the nasal discharge of a dead captive Cape ground squirrel (<i>Xerus inauris</i>), is a Gram-positive, oxidase-negative, catalase-positive and coagulase-negative coccoid bacterium. It grows under both aerobic and anaerobic conditions at 37 °C and tolerates up to 17.5% NaCl, and its colonies are smooth, convex and circular and exhibit complete staphylococcal <i>α</i>-haemolysis on sheep and horse blood agar. Phylogenomic analysis based on DNA alignment and amino-acid alignment obtained from the translated whole-genome sequence, along with comparisons of 16S rRNA and housekeeping genes (<i>hsp60</i>, <i>dnaJ</i>, <i>rpoB</i>, <i>sodA</i>, <i>gap</i> and <i>tuf</i>), placed strain 17KM0847^T within the genus <i>Staphylococcus</i>, most closely related to species of <i>Staphylococcus muscae</i>, <i>Staphylococcus microti</i>, <i>Staphylococcus rostri</i> and <i>Staphylococcus americanisciuri</i>. The strain exhibited digital DNA-DNA hybridization (dDDH) values ≤22% and average nucleotide identity (ANI) values <78% to these closest relatives, well below the species-level delineation thresholds (70% dDDH; 95% ANI). Chemotaxonomic analysis identified diphosphatidylglycerol, phosphatidylglycerol and an aminophospholipid as the major polar lipids. The cellular fatty acid profile was dominated by C_20 : 0, <i>iso</i>-C_15 : 0, C_16 : 0 and C_18 : 0. The peptidoglycan contained type A3<i>α</i>l-Lys-Gly₄-l-Ser-Gly. Based on chemotaxonomic and genomic characteristics, strain 17KM0847^T (=DSM 120281^T; =CCOS 2136^T; =CCM 9521^T) represents a novel species of the genus <i>Staphylococcus</i>, for which the name <i>Staphylococcus xeri</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Haloleucobacter ellipsoideus</i> gen. nov., sp. nov., and <i>Haloleucobacter albus</i> sp. nov., two novel N₂-fixing hydrogen bacteria isolated from soil near a wastewater treatment plant.","authors":"Xinyi Huang, Yue Su, Yusi Yang, Jiali Du, Hongzhe Hu, Shasha Qiu, Qiyun Zheng, Tao Wang, Can Chen","doi":"10.1099/ijsem.0.007270","DOIUrl":"https://doi.org/10.1099/ijsem.0.007270","url":null,"abstract":"<p><p>Two Gram-stain-negative, aerobic, oxidase-negative, non-motile, ellipsoid to short rod-shaped bacteria (~1.2-2.0 µm in length and 0.7-0.8 µm in width) were isolated from soil near a wastewater treatment plant in Hangzhou, Zhejiang Province, China, designated TB0136^T and TB0139^T. Both strains are able to grow chemolithoautotrophically using hydrogen as an electron donor and perform nitrogen fixation in the absence of ammonium or any organic nitrogen source. Strains TB0136^T and TB0139^T were observed to grow at temperatures of 15-45 °C (both optimal at 33 °C; TB0139^T additionally showed trace growth at 50 °C), at pH of 6.0-10.0 (TB0136^T optimal at 7.5-8.0, TB0139^T optimal at 8.0-8.5) and in the presence of 0-6.0% (w/v) NaCl (optimal at 1.0%). Chemotaxonomic analyses indicated that the main respiratory quinone in both strains is ubiquinone-10. The DNA G+C contents of strains TB0136^T and TB0139^T were 63.3 and 61.5 mol%, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence and the whole genome indicated that strains TB0136^T and TB0139^T formed a unique lineage in the evolutionary branches of the family <i>Xanthobacteraceae</i>. According to the characteristics of polyphasic taxonomy, strains TB0136^T and TB0139^T are proposed to represent two novel species of a new genus, for which the name <i>Haloleucobacter</i> gen. nov. is proposed. The proposed species names are <i>Haloleucobacter ellipsoideus</i> and <i>Haloleucobacter albus</i>. The type strains are TB0136^T (=MCCC 1K09187^T=JCM 37006^T) and TB0139^T (= MCCC1K09185^T=JCM 37007^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148764061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delineation of <i>Paraburkholderia tuberum</i> and description of <i>Paraburkholderia lebeckiae</i> sp. nov. isolated from the root nodules of South African <i>Lebeckia ambigua</i>.","authors":"Lazarus Mavima, Emma T Steenkamp, Tolu O Soogun, Chrizelle W Beukes, Marike Palmer, William B Whitman, John Howieson, Ron Yates, Euan K James, Sofie E De Meyer, Martin P A Coetzee, Stephanus N Venter","doi":"10.1099/ijsem.0.007262","DOIUrl":"https://doi.org/10.1099/ijsem.0.007262","url":null,"abstract":"<p><p><i>Paraburkholderia tuberum</i> is a diverse beta-rhizobial species (i.e. rhizobia bacteria belonging to the class <i>Betaproteobacteria</i>) indigenous to South Africa that associates mostly with indigenous papilionoid legume species. The taxonomic boundary of this species was reviewed in 2022, which indicated that it includes a number of diverse strains. In fact, some of these <i>P. tuberum</i> strains appear to have sufficient genetic heterogeneity to belong to separate species. The absence of publicly available whole-genome sequences for the majority of <i>P. tuberum</i> strains during the last review of their taxonomy limited the accuracy of their species delineation. In this study, we revise the species delineation of <i>P. tuberum</i> using a comprehensive genome-based approach. With whole-genome sequences of 28 <i>P</i>. <i>tuberum</i> strains now available, we performed genealogical concordance analysis based on 92 conserved gene regions and conducted analysis of average nucleotide identity, digital DNA-DNA hybridization and pangenome coding content. Overall, our analyses grouped the 28 <i>P</i>. <i>tuberum</i> strains into two separate species clusters, which showed sufficient divergence to allow recognition as separate species. The one cluster represents <i>P. tuberum sensu stricto</i>, while the second cluster was described as a novel species. For this novel species, we propose the name <i>Paraburkholderia lebeckiae</i> sp. nov., with the type strain WSM4175^T (=CMW-IA:007206^T; DSM 120862^T; LMG 34098^T). Our results confirm that whole-genome-based information is crucial in determining accurate species boundaries, and its use should be supported and promoted.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148793026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cutibacterium nasicola</i> sp. nov., isolated from nasal swabs of coal miners.","authors":"Junyao Xie, Tao Liu, Mei Zhang, Fangyu Gao, Lijun Zhao, Taoyu Yan, Zixin Weng, Qiang Yang, Caixin Yang, Zhiming Kang, Ji Pu, Han Zheng, Yiwei Shi, Jianguo Xu, Kui Dong","doi":"10.1099/ijsem.0.007267","DOIUrl":"10.1099/ijsem.0.007267","url":null,"abstract":"<p><p>Two Gram-stain-positive, catalase-positive, oxidase-negative, non-spore-forming, aerotolerant anaerobic and non-motile short rod-shaped bacterial strains, designated V947^T and V970, were isolated from nasal swab samples of coal miners. Phylogenetic analyses based on 16S rRNA gene sequences and whole-genome sequences revealed that strains V947^T and V970 represent a distinct lineage within the genus <i>Cutibacterium</i>, most closely related to <i>Cutibacterium avidum</i> ATCC 25577^T (16S rRNA gene sequence similarity of 97.09%). Whole-genome comparative analyses showed that the average nucleotide identity values between the two strains and all validly published species of the genus <i>Cutibacterium</i> with correct nomenclature ranged from 76.55 to 90.26%, while the digital DNA-DNA hybridization values ranged from 21.80 to 40.40%, both of which are well below the accepted thresholds for species delineation. Pangenome analysis identified 711 species-specific gene clusters present in strains V947^T and V970 but absent from all reference type strains of the genus, which were predominantly involved in carbohydrate transport and metabolism, inorganic ion transport and signal transduction mechanisms, suggesting distinct metabolic capabilities and potential for environmental adaptation. These genomic features not only support the delineation of a novel species but also expand the known genomic and functional diversity within the genus <i>Cutibacterium</i>. The predominant cellular fatty acids were iso-C_15:0 and anteiso-C_15:0. The major polar lipids of strain V947^T were diphosphatidylglycerol, phosphatidylinositol and phosphatidylcholine, and the predominant menaquinones were MK-8(H₄) and MK-9(H₆). On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic characteristics, strains V947^T and V970 represent a novel species of the genus <i>Cutibacterium</i>, for which the name <i>Cutibacterium nasicola</i> sp. nov. is proposed. The type strain is V947^T (=CGMCC 1.5844^T=KCTC 59608^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Aleya cavernae</i> gen. nov., sp. nov., and <i>Lacrimispora cavernae</i> sp. nov., novel taxa isolated from bat guano.","authors":"Samuel L Miller, Nam Lu, Payton R Shears, Jacobey King, Krithivasan Sankaranarayanan, Paul A Lawson","doi":"10.1099/ijsem.0.007260","DOIUrl":"https://doi.org/10.1099/ijsem.0.007260","url":null,"abstract":"<p><p>Bats, of the order <i>Chiroptera</i>, comprise over 1,400 species and account for over 21% of all currently described mammalian species. However, despite this huge group of mammals, a remarkably small number of bacterial species have been described from this group. In a continuing study of non-human mammals, dozens of strains were isolated from anaerobic guano-pile enrichments collected in a bat cave near Protem, MO, USA. Of these, two novel taxa were identified and designated BS-2^T and BS-20^T; both were rod-shaped, Gram-stain-positive bacteria. Phylogenetic analysis of the 16S rRNA gene and phylogenomic analysis placed BS-2^T within the genus <i>Lacrimispora</i>, and BS-20^T was located within the family <i>Jonesiaceae</i>, but with no particular affiliation to any genus. Overall genomic-based relatedness indices indicated that strains BS-2^T and BS-20^T shared average nucleotide identity and digital DNA-DNA hybridization values <95.0% and <70.0% with their nearest neighbouring taxa, which are the currently accepted thresholds for species-level delineation. Further, for strain BS-20^T, genus-specific delineation indices demonstrated a shared average amino acid identity, and percentages of conserved proteins were <68.0% and <42.0%, respectively, between BS-20^T and other <i>Jonesiaceae</i> taxa, below commonly accepted genus-level delineation thresholds. The predominant cellular fatty acid of isolate BS-2^T was C_16:0, whereas BS-20^T contained C_15:0 anteiso and C_14:0 as the predominant cellular fatty acids. The G+C content of BS-2^T and BS-20^T was 44.1 and 56.5 mol%, respectively. Based on biochemical, phylogenetic, genotypic and chemotaxonomic criteria, strains BS-2^T and BS-20^T represent novel isolates within the genus <i>Lacrimispora</i> and family <i>Jonesiaceae</i>, for which the names <i>Lacrimispora cavernae</i> sp. nov. and <i>Aleya cavernae</i> gen. nov., sp. nov. are proposed. Strains BS-2^T (=NRRL B 65694^T=CCUG 77247^T=CCM 9410^T) and BS-20^T (=NRRL B-65699^T=CCUG 77269^T) are proposed as the type species of these novel taxa.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13488357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Leucobacter shiyiweii</i> sp. nov. and <i>Leucobacter fengyongliangi</i> sp. nov., isolated from coal samples in Shanxi Province of China.","authors":"Junyao Xie, Yihang Yang, Fangyu Gao, Lijun Zhao, Tao Liu, Caixin Yang, Zhiming Kang, Ji Pu, Han Zheng, Jianguo Xu, Kui Dong","doi":"10.1099/ijsem.0.007271","DOIUrl":"https://doi.org/10.1099/ijsem.0.007271","url":null,"abstract":"<p><p>Four novel bacterial strains representing two novel species were isolated from <i>in situ</i> coal samples. Comparative analysis of 16S rRNA gene sequences showed that strains W1153^T and W1478 share the highest sequence similarity with <i>Leucobacter tardus</i> K 70/01^T (98.3%), whereas strains Z1108^T and W1038 share the highest sequence similarity with <i>L. tardus</i> K 70/01^T and <i>Leucobacter exalbidus</i> K-540B^T (98.0%). Phylogenetic analysis based on 16S rRNA gene sequences and a core-genome phylogeny constructed from 665 core genes demonstrated that the four strains form two distinct clades, clearly separated from other species within the genus <i>Leucobacter</i>. Whole-genome sequencing revealed genome sizes ranging from 2.6 to 2.8 Mb, with G+C contents of 62.5-63.5 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between the four strains and all recognized species within the genus <i>Leucobacter</i> ranged from 71.1 to 75.6% and 19.3 to 25.6%, respectively. The major cellular fatty acids of the four strains were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and iso-C_{16 : 0}. The respiratory quinone types were MK-11 for strain W1153^T and MK-9(H8) for strain Z1108^T. The main cell-wall sugars were rhamnose and ribose, with 2,4-diaminobutyric acid present as the diagnostic diamino acid. Based on phenotypic characteristics, phylogenetic analyses and chemotaxonomic evidence, strains W1153^T and W1478, and Z1108^T and W1038, are proposed as representing two novel species within the genus <i>Leucobacter</i>, named <i>Leucobacter shiyiweii</i> sp. nov. and <i>Leucobacter fengyongliangi</i> sp. nov., respectively. The type strains are W1153^T (=GDMCC 1.5845^T=KCTC 59606^T) and Z1108^T (=GDMCC 1.5846^T=KCTC 59607^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148828614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Altererythrobacter chaiwopuensis</i> sp. nov., a novel alphaproteobacterial strain isolated from sediment of the Chaiwopu Lake.","authors":"Xin-Yao Li, Yi-Fei Yang, Yi-Wen Liang, Ze-Xian Wu, Xu-Rui Li, Shuai Li, Yong-Hong Liu, Lei Gao, Ting-Ting She, Bao-Zhu Fang, Wen-Jun Li","doi":"10.1099/ijsem.0.007250","DOIUrl":"10.1099/ijsem.0.007250","url":null,"abstract":"<p><p>Two alphaproteobacterial strains, designated EGI L300112^T and EGI L300120, were isolated from sediment collected from Chaiwopu Lake in Xinjiang, China. The taxonomic position of the two strains was determined using polyphasic taxonomic analysis and phylogenomic analysis. Based on 16S rRNA gene sequence similarities, strain EGI L300112^T and EGI L300120 were both closely related to <i>Pelagerythrobacter rhizovicinus</i> AY-3R^T and shared the highest sequence identities of 96.2% and 96.3%, respectively. However, phylogenetic analysis based on the 16S rRNA gene and genomes clearly demonstrated that the two strains formed a distinct clade with validly published species of the genus <i>Altererythrobacter</i>. Furthermore, the two strains exhibited distinct phenotypic, physiological and genotypic characteristics that differentiate them from other related type species of the genus <i>Altererythrobacter</i> and related species in the family <i>Erythrobacteraceae</i>. Cells of the two strains were aerobic, Gram-stain negative, non-motile and rod-shaped. Optimal growth conditions for EGI L300112^T and EGI L300120 occurred on marine agar 2216 at pH 7 at 30 °C. The major respiratory quinone was Q-10. The detected polar lipids of two strains included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine and unidentified phospholipids. The major fatty acids (>10%) were identified as summed feature 3 (C_16 : 1 <i>ω</i>7<i> c</i>/C_16 : 1 <i>ω</i>6 c) and summed feature 8 (C_18 : 1 <i>ω</i>7 c). The G+C content of strain EGI L300112^T and EGI L300120 was 61.2% and 61.4%, respectively. Based on differential phenotypic and genotypic characteristics of the two strains and related species in the genus <i>Altererythrobacter</i>, the two strains should be classified as representing a new species of this genus, for which the name <i>Altererythrobacter chaiwopuensis</i> sp. nov. is proposed. The type strain is EGI L300112^T (=MCCC 1K09432^T=KCTC 8712^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13460513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}