International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Vallitalea sediminicola</i> sp. nov. and <i>Vallitalea maricola</i> sp. nov., anaerobic bacteria isolated from deep-marine sediment.","authors":"Shiori Hirano, Anna Maeda, Takeshi Terahara, Koji Mori, Moriyuki Hamada, Takamasa Miura, Ryo Matsumoto, Takeshi Kobayashi","doi":"10.1099/ijsem.0.006721","DOIUrl":"10.1099/ijsem.0.006721","url":null,"abstract":"<p><p>Two obligately anaerobic, Gram-stain-negative, non-spore-forming, rod-shaped, motile and mesophilic bacteria, designated as strains AN17-1^T and AN17-2^T, were isolated from deep-marine sediments collected from the western Pacific Ocean. The phylogenetic analysis based on the 16S rRNA gene with the most closely related species showed 98.5 and 98.8% similarities of strains AN17-1^T and AN17-2^T with <i>Vallitalea guaymasensis</i> Ra1766G1^T, respectively. The genome sizes and G+C contents of strains AN17-1^T and AN17-2^T were 6.0 Mb and 30.3 mol% and 5.4 Mb and 30.9 mol%, respectively. The genome-to-genome distance and the average nucleotide identity values between strains AN17-1^T and AN17-2^T and <i>V. guaymasensis</i> Ra1766G1^T were lower than the thresholds for species delimitation. Elemental sulphur, sulphate, thiosulphate, sulphite, fumarate, nitrate and nitrite were not used as terminal electron acceptors by strains AN17-1^T and AN17-2^T. The most abundant fatty acids of strains AN17-1^T and AN17-2^T were iso-C_15:0 and C_16:0, respectively. The commonly detected polar lipids of both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid, two unidentified phospholipids and one unidentified polar lipid. The results suggest that strains AN17-1^T and AN17-2^T represent novel species of the genus <i>Vallitalea</i>. Therefore, the new names, <i>Vallitalea sediminicola</i> sp. nov. and <i>Vallitalea maricola</i> sp. nov., are proposed for strains AN17-1^T (=NBRC 115486^T=DSM 116091^T) and AN17-2^T (=NBRC 115487^T=DSM 116092^T) as the type strains, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics as baseline for taxonomy description: <i>Amphibiibacter pelophylacis</i> gen. nov.<i>,</i> sp. nov., a novel taxon of the family <i>Sphaerotilaceae</i>, class <i>Betaproteobacteria,</i> isolated from the skin microbiota of <i>Pelophylax perezi</i> from different populations.","authors":"Sara Costa, Diogo Neves Proença, Isabel Lopes, Paula V Morais","doi":"10.1099/ijsem.0.006741","DOIUrl":"https://doi.org/10.1099/ijsem.0.006741","url":null,"abstract":"<p><p>Bacterial strain SL12-8^T was characterized and isolated from the skin microbiota of <i>Pelophylax perezi</i>, the Perez's frog. Strain SL12-8^T stained Gram-negative and formed rod-shaped cells that grew optimally at 25 °C and pH 7.0-7.5. The G+C content of the DNA was 66.2 mol%. Ubiquinone 8 was the respiratory quinone identified in the studied strain and the most closely related taxon. The major fatty acids were summed in feature 3 and summed in feature 8 and C_16:0, representing 84% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain SL12-8^T within the order <i>Burkholderiales</i> in a distinct lineage. The 16S rRNA gene sequence similarities of strain SL12-8^T to that of <i>Rubrivivax albus</i>, <i>Scleromatobacter humisilvae</i>, <i>Piscinibacter aquaticus</i>, <i>Azohydromonas caseinilytica</i> and <i>Aquincola agrisoli</i> were 94.41, 94.08, 93.72, 93.72 and 93.64%, respectively. The draft genome sequence of strain SL12-8^T comprises 3,115,197 bases with a 313-fold mapped genome coverage. The assembled genome consists of 53 large contigs with more than 500 bp, and the genome encodes 2,814 putative coding sequences. The analysis of the available genomes from the closest genera showed 124 core genes that reveal a novel genus-level clade including the strain SL12-8^T. Analysis of the SL12-8^T genome revealed the presence of the beta-lactone and terpene biosynthetic gene clusters. The phylogenomic, phylogenetic, phenotypic and chemotaxonomic data showed that strain SL12-8^T (=UCCCB 131^T=CECT 30762^T) represents the type of a novel species and genus, for which we propose the name <i>Amphibiibacter pelophylacis</i> gen. nov., sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Marinicellulosiphila megalodicopiae</i> gen. nov., sp. nov., a deep-sea alkaliphilic cellulolytic bacterium isolated from an endemic ascidian <i>Megalodicopia hians</i>.","authors":"Mikako Tachioka, Masayuki Miyazaki, Mikiko Tsudome, Miwako Tsuda, Kohsuke Uchimura, Yoshihiro Takaki, Shigeru Deguchi","doi":"10.1099/ijsem.0.006742","DOIUrl":"10.1099/ijsem.0.006742","url":null,"abstract":"<p><p>The strain TOYAMA8^T is a deep-sea alkaliphilic cellulolytic bacterium isolated from a slurry-adhered epiphytic site of <i>Megalodicopia hians</i>. Cells of this strain are Gram-negative, aerobic, curved rods or spirilla, motile with monopolar flagella, and grow on cellulose as the sole carbon source. Compared to other closely related species, this bacterium is characterized by a large number of cellulase genes. Strain TOYAMA8^T showed alkaliphilic growth within the pH range 7.5-9.0. The major cellular fatty acids were C_18 : 1 ω7, C_14 : 0, C_16 : 0 and C_16 : 1 ω7. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipids and aminolipids. A major respiratory lipoquinone was Q-9. Phylogenomic analysis using the 16S rRNA gene and whole-genome sequence data showed that the strain is related to the families <i>Gynuellaceae</i>, <i>Saccharospirillaceae</i> and <i>Natronospirillaceae</i>. The values of 16S rRNA gene sequence similarity, amino acid identity and percentage of conserved proteins between the strain TOYAMA8^T and related species were low, with maximum values of 90.6, 48.1 and 34.6%, respectively. These results, together with differences in phenotypic and biochemical characteristics, indicate that the new isolate TOYAMA8^T represents a novel genus and species, for which the name <i>Marinicellulosiphila megalodicopiae</i> gen. nov., sp. nov., is proposed. The type strain is TOYAMA8^T (JCM 31119^T=DSM 114864^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nocardia arseniciresistens</i> sp. nov., isolated from lead-zinc mine tailing.","authors":"Xiao-Ming Wang, Xiu Chen, Guo-Xing Zhu, Chun-Mei Lu, Yuan-Chun Yang, Jing Zhang, Hao-Tian Dong, Sheng-Guang Xu, Lian-Ming Liang, Shu-Kun Tang, Yan-Ru Cao","doi":"10.1099/ijsem.0.006738","DOIUrl":"https://doi.org/10.1099/ijsem.0.006738","url":null,"abstract":"<p><p>A strain with aerial hyphae presented, designated KC 131^T, was isolated from lead-zinc mine tailings, Lanping, Yunnan province, Southwest China. The strain was Gram-stain-positive, acid-alcohol-fast, aerobic and non-motile. Based on 16S rRNA gene sequence and phylogeny analysis, strains <i>Nocardia ninae</i> NBRC 108245^T (98.4%), <i>Nocardia alba</i> YIM 30243^T (98.4%) and <i>Nocardia colli</i> CICC 11023^T (98.2%) were the most closely related species of strain KC 131^T, and the four strains were in one cluster. The <i>meso</i>-diaminopimelic acid, arabinose and galactose were detected in the whole-cell hydrolysates. The mycolic acids were presented, and the major fatty acids (>5%) were C_16:0 (30.0%), C_18:1 ω9c (12.5%), C_18:0 (7.5%), 10-methyl C_18:0 (27.3%) and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c; 12.4%). The menaquinone was MK-8 (H4, ω-cyclo), and the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. All these features were consistent with the genus <i>Nocardia</i>. Digital DNA-DNA hybridization values between strain KC 131^T and <i>N. ninae</i> NBRC 108245^T, <i>N. alba</i> DSM 44684^T and <i>N. colli</i> CICC 11023^T were 23.8%, 20.9% and 23.5%, respectively. The average nucleotide identity values between strain KC 131^T and the three strains were 80.0%, 76.5% and 80.0%, respectively. In addition, some physiological and biochemical characteristics were also different. Thus, strain KC 131^T represents a novel species of the genus <i>Nocardia</i>, for which the name <i>Nocardia arseniciresistens</i> sp. nov. is proposed. The type strain is KC 131^T (=CGMCC 1.19493^T=JCM 35916^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the priority between <i>Yokenella regensburgei</i> Kosako <i>et al</i>. 1985 and <i>Koserella trabulsii</i> Hickman-Brenner <i>et al</i>. 1985 and on the priority between <i>Providencia alcalifaciens</i> (de Salles Gomes 1944) Ewing 1962 (Approved Lists 1980) and <i>Proteus inconstans</i> (Ornstein 1920) Shaw and Clarke 1955 (Approved Lists 1980). Requests for an Opinion.","authors":"Meng-Syun Li","doi":"10.1099/ijsem.0.006746","DOIUrl":"10.1099/ijsem.0.006746","url":null,"abstract":"<p><p>In this paper, I include two Requests for an Opinion regarding ambiguous priority of pairs of names considered synonyms. First, I present a case of unknown priority between heterotypic synonyms <i>Yokenella regensburgei</i> Kosako <i>et al</i>. 1985 and <i>Koserella trabulsii</i> Hickman-Brenner <i>et al</i>. 1985. These two names appeared on the same Validation List but without any sequence number given. According to Rule 24b(4) of the 2022 Revision of the International Code of Nomenclature of Prokaryotes (ICNP), in the case of no sequence numbers given, it is up to those who first propose union of the taxa to determine the priority. However, no choice was made by those who synonymized these two names, and therefore, it is impossible to choose the correct name. To resolve this extremely rare, awkward situation, I argue that the Judicial Commission should conserve the genus name and epithet of the well-known name <i>Y. regensburgei</i> over those of the less-known <i>K. trabulsii</i> according to Rule 56b of the ICNP. And by doing this, <i>Y. regensburgei</i> will become the correct name for the united taxon with this particular circumscription. Secondly, I discussed the case in which <i>Providencia alcalifaciens</i> (de Salles Gomes 1944) Ewing 1962 (Approved Lists 1980) and <i>Proteus inconstans</i> (Ornstein 1920) Shaw and Clarke 1955 (Approved Lists 1980) are found to be homotypic synonyms both included in the Approved Lists. Based on the priority, <i>inconstans</i> in <i>P. inconstans</i> is the earliest epithet to be adopted in the correct name, which means that <i>alcalifaciens</i> in <i>P. alcalifaciens</i> should be treated as an illegitimate epithet. Since <i>P. alcalifaciens</i> was proposed as the type species of the genus <i>Providencia</i>, the generic name and any of its combinations are illegitimate as well according to Rules 20a and 51b(2) of the ICNP. Literature search showed that the name <i>Proteus inconstans</i> has become obsolete and <i>Providencia alcalifaciens</i> is in prevailing usage. Moreover, 16 validly published names, including synonyms, have been proposed to date under the genus <i>Providencia</i>. Accordingly, I argue that the necessary conservation of the epithet <i>inconstans</i> in <i>P. inconstans</i> over <i>alcalifaciens</i> in <i>P. alcalifaciens</i> should be made by the Judicial Commission according to Rule 56b for the sake of nomenclatural stability.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>Pasteurella skyensis</i> as <i>Phocoenobacter skyensis</i> comb. nov. and description of <i>Phocoenobacter atlanticus</i> sp. nov. isolated from diseased Atlantic salmon (<i>Salmo salar</i>) and lumpfish (<i>Cyclopterus lumpus</i>), with subdivision into <i>Phocoenobacter atlanticus</i> subspecies <i>atlanticus</i> subsp. nov. and <i>Phocoenobacter atlanticus</i> subspecies <i>cyclopteri</i> subsp. nov.","authors":"Hanne K Nilsen, Anne Berit Olsen, Thomas H Birkbeck, Farah Manji, Duncan J Colquhoun, Snorre Gulla","doi":"10.1099/ijsem.0.006729","DOIUrl":"https://doi.org/10.1099/ijsem.0.006729","url":null,"abstract":"<p><p>Gram-negative bacteria, similar in 16S rRNA gene sequence to <i>Pasteurella skyensis</i>, have been irregularly cultured from diseased Atlantic salmon (<i>Salmo salar</i>) sea-farmed in Norway since the 1980s and more commonly in later years from diseased lumpfish (<i>Cyclopterus lumpus</i>) farmed in the northeast Atlantic area. Phenotypic and genetic analyses confirmed that the majority of Norwegian isolates in the present study, cultured from both salmon and lumpfish, are closely related to, yet distinct from, <i>P. skyensis</i> and represent a hitherto undescribed species. We further argue, based on genetic similarity levels, that the original placement of <i>P. skyensis</i> within <i>Pasteurella</i> was erroneous and that this species belongs within <i>Phocoenobacter</i>, as <i>Phocoenobacter skyensis</i> comb. nov. Accordingly, we propose the name <i>Phocoenobacter atlanticus</i> sp. nov. for the new species characterized here. Furthermore, this species separates into two genetically and phenotypically distinct lineages, respectively, associated with Atlantic salmon and lumpfish, and for which we propose the names <i>Ph. atlanticus</i> subsp. <i>atlanticus</i> subsp. nov. and <i>Ph. atlanticus</i> subsp. <i>cyclopteri</i> subsp. nov., respectively. The type strain for <i>Ph. atlanticus</i> subsp. <i>atlanticus</i> subsp. nov. is strain NVIB3624^T (NCIMB 15512^T, CCUG 77520^T), and the type strain for <i>Ph. atlanticus</i> subsp. <i>cyclopteri</i> subsp. nov. is strain NVIO9100^T (NCIMB 15511^T, CCUG 77521^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Setting new boundaries of 16S rRNA gene identity for prokaryotic taxonomy.","authors":"Timothy J Hackmann","doi":"10.1099/ijsem.0.006747","DOIUrl":"10.1099/ijsem.0.006747","url":null,"abstract":"<p><p>The 16S rRNA gene is frequently sequenced to classify prokaryotes and identify new taxa. If sequences from two strains share less than ~99% identity, the strains are usually classified as different species. Classification thresholds for genera and other ranks have also been proposed, but they are based on dated datasets. Here we update these thresholds by determining the sequence identity of the 16S rRNA gene for <i>n</i>=19,556 type strains. This represents 94% of all strains validly published, and it involved making more than 191 million pairwise sequence alignments. In 90% of all cases, sequences from the same species shared a minimum of 97.2-100% identity. The corresponding values were 90.1-99.0% for genus, 80.1-94.1% for family, 72.9-90.0% for order, 72.2-86.3% for class and 69.6-83.6% for phylum. We also present values specific to bacteria (<i>n</i>=18,904 strains) and archaea (<i>n</i>=652 strains). We propose these values serve as thresholds for classifying new prokaryotic taxa. A major change from previous guidelines is recognizing that these boundaries overlap. This overlap has already been observed for relative evolutionary divergence, a metric correlated with 16S rRNA gene identity. Together with other metrics, 16S rRNA gene identity allows classification of prokaryotes from species to phylum.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Sinomonas puerhi</i> sp. nov. isolated from the rhizosphere soil of Pu-erh tea based on whole-genomic profiling.","authors":"Jianyang Fu, Yan Shen, Shengjie Duan, Huiqing Luo, Xinrui Ma, Liang Yan, Chongye Fang, Ruijuan Yang","doi":"10.1099/ijsem.0.006752","DOIUrl":"https://doi.org/10.1099/ijsem.0.006752","url":null,"abstract":"<p><p>An aerobic, Gram-stain-positive, rod-shaped and non-motile bacterium, designated strain P10A9, was isolated from the rhizosphere soil of Pu-erh tea plants (<i>Camellia sinensis</i> var. <i>assamica</i>) in an organic tea garden in the Jingmai Pu-erh Tea District, Pu'er City, Yunnan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P10A9 belongs to the genus <i>Sinomonas</i>, with its closest relative being <i>Sinomonas atrocyanea</i> KCTC 3377^T (98.4% similarity). The major fatty acids (>10.0% of the total) were anteiso-C_{15 : 0} and anteiso-C_17:0. The predominant respiratory quinone was MK-9(H₂), with MK-8(H₂) as a minor component. The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and glycolipids. The peptidoglycan contained glutamic acid (Glu), lysine (Lys), aspartic acid (Asp), serine (Ser), alanine (Ala) and glycine (Gly). The genome of strain P10A9 is 4.3 Mbp in size, with a G+C content of 69.2 mol%. Digital DNA-DNA hybridization values between strain P10A9 and other <i>Sinomonas</i> species ranged from 20.8 to 25.1%, and the average nucleotide identity values were significantly below the species delineation threshold (95-96%). Based on these results, strain P10A9 represents a novel species of the genus <i>Sinomonas</i>, for which the name <i>Sinomonas puerhi</i> sp<i>.</i> nov. is proposed. The type strain is P10A9^T (=CCTCC AB 2024154^T=KCTC 59368^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposal of <i>Actinoplanes capillaceus</i> Matsumoto <i>et al</i>. 2000 as a later heterotypic synonym of <i>Actinoplanes campanulatus</i> (Couch 1963) Stackebrandt and Kroppenstedt 1987 and an emended description of <i>Actinoplanes campanulatus</i>.","authors":"Ping Mo, Yiru Ming, Yuhuan Cao, Kaiqin Li, Wansheng Zou, Yun Wang, Baiyuan Li","doi":"10.1099/ijsem.0.006744","DOIUrl":"https://doi.org/10.1099/ijsem.0.006744","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ureaplasma ceti</i> sp. nov. isolated from the gastric fluid of a spotted dolphin (<i>Stenella attenuata</i>).","authors":"Takao Segawa, Yu Nakajima, Keiko Yamamoto, Shoko Kishima, Takuya Itou","doi":"10.1099/ijsem.0.006712","DOIUrl":"https://doi.org/10.1099/ijsem.0.006712","url":null,"abstract":"<p><p><i>Ureaplasma</i> sp. (OM1^T, OM4 and OM7) novel strains were isolated from the gastric fluid of a spotted dolphin (<i>Stenella attenuata</i>). These strains were phenotypically and genotypically characterized, and compared with known species of the genus <i>Ureaplasma</i>. All isolated strains hydrolysed urea and metabolized arginine, but did not produce acid from glucose. All strains were propagated using pleuropneumonia-like organisms medium supplemented with serum and urea under aerobic and anaerobic atmospheric conditions at 37 °C. Transmission electron microscopy revealed a typical mollicute cellular morphology. Analysis of the 16S rRNA gene sequence indicated that the most closely related, validly named type strain was <i>Ureaplasma gallorale</i> ATCC 43346^T (89.4% similarity). The average nucleotide identity and digital DNA-DNA hybridization values among strain OM1^T and closely related species were lower than the accepted thresholds for describing novel prokaryotic species at the genomic level. Based on the genomic, phenotypic and phylogenetic properties, the strains represent a novel species of the genus <i>Ureaplasma</i>, for which the name <i>Ureaplasma ceti</i> sp. nov. with type strain OM1^T (=DSM 116106^T=JCM 39153^T) is proposed. The genomic G+C content and draft genome sizes of the type strain were 31.7% and 889 711 bp, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}