International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Clavibacter vidaverae</i> sp. nov., <i>Clavibacter rahimiani</i> sp. nov. and <i>Clavibacter davisi</i> sp. nov.: corynebacterial plant pathogens isolated from small-grain cereals.","authors":"Asma Rahmanzadeh, S Mohsen Taghavi, Habibeh Hajian-Maleki, Hamid Abachi, Nastaran Zamani, Sadegh Zarei, Céline Mirguet, Martial Briand, Xiang Li, Nicolas W G Chen, Marie-Agnès Jacques, Perrine Portier, Ebrahim Osdaghi","doi":"10.1099/ijsem.0.007154","DOIUrl":"https://doi.org/10.1099/ijsem.0.007154","url":null,"abstract":"<p><p>During 2020-2024, 25 Gram-positive actinobacterial strains were isolated from small-grain cereals in Iran. Colony morphology, phenotypic characteristics and multilocus sequence analyses showed that the strains belonged to the coryneform genus <i>Clavibacter</i> (family <i>Microbacteriaceae</i>). The <i>Clavibacter</i> strains isolated from small-grain cereals in Iran were distributed within five distinct phylogenetic clades. Two of those clades included <i>Clavibacter tessellarius</i> and <i>Clavibacter zhangzhiyongii</i>, while the other three groups of <i>Clavibacter</i> strains were taxonomically undetermined and phylogenetically distinct from all validly described species in the genus. Whole-genome sequence-based indices, i.e. average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), showed that the latter three phylogenetic clades differed from all validly described <i>Clavibacter</i> species, with ANI/dDDH values <94% and <70%, respectively. Analyses of KEGG pathways and pathogenicity-associated genes revealed substantial differences among the five <i>Clavibacter</i> groups associated with small-grain cereals. All <i>Clavibacter</i> strains were capable of infecting their hosts of isolation, i.e. wheat, barley and oat. Thus, based on both phenotypic features, i.e. differences in colony morphology, biolog microbial identification microplate data and polar lipid repertoires, as well as DNA similarity indices, the three clades could represent three new species within <i>Clavibacter</i>. In this study, we provide formal species descriptions for three <i>Clavibacter</i> clades isolated from small-grain cereals. These three species include <i>Clavibacter vidaverae</i> sp. nov. (type strain Sh2088ᵀ=CFBP 9070ᵀ=ICMP 24732ᵀ), <i>Clavibacter rahimiani</i> sp. nov. (type strain Sh2141ᵀ=CFBP 9069ᵀ=ICMP 24735ᵀ) and <i>Clavibacter davisi</i> sp. nov. (type strain Sh2036ᵀ=CFBP 9071ᵀ=ICMP 24729ᵀ).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics-based reclassification of <i>Brevibacterium celere</i> and <i>Brevibacterium sandarakinum</i> as later heterotypic synonyms of <i>Brevibacterium sanguinis</i> and <i>Brevibacterium picturae</i>, respectively.","authors":"Jasvinder Kaur, Jaspreet Kaur, Manali Bhardwaj, Vaishnavi Rajagopalan","doi":"10.1099/ijsem.0.007145","DOIUrl":"10.1099/ijsem.0.007145","url":null,"abstract":"<p><p>The present study used genome-based approaches to clarify the taxonomic status of four closely related species of the genus <i>Brevibacterium</i>. At the time of this study, genomic information for 36 validly published type strains with correct names was available. The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization values for <i>Brevibacterium celere</i> KMM 3637^T and <i>Brevibacterium sanguinis</i> CF63^T are 99.26%, 98.64% and 88.20%, respectively. For the second pair, <i>Brevibacterium sandarakinum</i> 01-Je-003^T and <i>Brevibacterium picturae</i> LMG 22061^T, these values are 97.97%, 97.81% and 81.80%, respectively. These findings indicate that <i>B. celere</i> KMM 3637ᵀ and <i>B. sanguinis</i> CF63ᵀ belong to the same species, as do <i>B. sandarakinum</i> 01-Je-003ᵀ and <i>B. picturae</i> LMG 22061ᵀ. Based on these evidences, we propose the reclassification of <i>Brevibacterium celere</i> Ivanova <i>et al</i>. 2004 and <i>Brevibacterium sandarakinum</i> Kämpfer <i>et al</i>. 2010 as later heterotypic synonyms of <i>Brevibacterium sanguinis</i> Wauters <i>et al</i>. 2004 and <i>Brevibacterium picturae</i> Heyrman <i>et al</i>. 2004, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive genome-centred taxonomy for agrobacteria and rhizobia in the <i>Bartonellaceae</i> and <i>Rhizobiaceae</i> families.","authors":"Melandré van Lill, Stephanus N Venter, Marike Palmer, Chrizelle W Beukes, Emma T Steenkamp","doi":"10.1099/ijsem.0.007125","DOIUrl":"https://doi.org/10.1099/ijsem.0.007125","url":null,"abstract":"<p><p>To clarify the taxonomy of agrobacteria and rhizobia - assemblages of bacteria critical for sustainable agriculture and biotechnology - we applied a phylogenomic approach to reassess their current classifications. By phylogenetically analysing two marker gene sets based on both nucleotide (UBCG) and amino acid levels (bac120) of the type strains within the families <i>Bartonellaceae</i> and <i>Rhizobiaceae</i>, we found that both marker sets led to congruent phylogenies that consistently recovered the same lineages. These generic boundaries of the proposed lineages were strongly supported by average amino acid identities of ≥76%. Our results corroborate with previously proposed genus-level thresholds and provided a robust foundation for taxonomic revision. Several taxonomic changes are proposed to minimize the disruption to current classifications while also reflecting the evolutionary histories of these organisms. These include the emendation of '<i>Paramesorhizobium</i>' to better reflect its distinction from <i>Ochrobactrum</i> and <i>Phyllobacterium</i>. Two <i>Rhizobium</i> species were also reassigned to the novel genera <i>Fluviirhizobium</i> gen. nov. and <i>Phytoamicus</i> gen. nov., reflecting the deep phylogenetic divergence and distinct ecological origins. Other taxonomic refinements include new combinations for species previously assigned to <i>Rhizobium</i> and <i>Mesorhizobium</i>, now shown to belong to <i>Paenirhizobium</i> and <i>Terribium</i>. We further clarified the classifications of <i>Allorhizobium</i>, <i>Neorhizobium</i> and <i>Mesorhizobium sensu</i> GTDB that were based on relative evolutionary divergence and were likely too broad. This demonstrates the need for an integrative genome-based approach to classify organisms that do not solely rely on numerical thresholds to produce biologically meaningful groups that enhance taxonomic stability.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Formosa sejongensis</i> sp. nov. and <i>Polaromonas potterensis</i> sp. nov., isolated from King George Island, Antarctica.","authors":"Sumin Choi, Hyeon Seop Lee, Sinseong Park, Seong Yeol Choi, Wonsik Mun, Kyoung Lee, Jin Hyung Lee, Jeong-Eun Han, Robert J Mitchell","doi":"10.1099/ijsem.0.007150","DOIUrl":"10.1099/ijsem.0.007150","url":null,"abstract":"<p><p>Gram-negative, rod-shaped bacteria, designated as strains PL04ᵀ and SM01ᵀ, were isolated from King George Island, Antarctica. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain PL04ᵀ belongs to the genus <i>Formosa</i> within the family <i>Flavobacteriaceae</i>. Pairwise 16S rRNA gene sequence comparison showed that <i>Formosa arctica</i> IMCC 9458ᵀ was the closest recognized relative (98.34% sequence similarity). Likewise, 16S rRNA gene sequence-based phylogenetic analysis revealed that strain SM01ᵀ is affiliated with the genus <i>Polaromonas</i> within the family <i>Comamonadaceae</i>, showing the highest sequence similarity to <i>Polaromonas ginsengisoli</i> Gsoil 115ᵀ (98.42%). The genomic DNA G+C contents of strains PL04ᵀ and SM01ᵀ were 32.8% and 61.81%, respectively. The major cellular fatty acids of PL04ᵀ and SM01^T were iso-C_15:0, iso-C_15:1 G [double peak identified as C_15:1 iso <i>ω</i>5<i>c</i> and C_15:1 iso <i>ω</i>10<i>c</i> by GC-mass spectrometry (MS)] and C_₁₆:0, summed feature 3 (identified as C_₁₆:1  <i>ω</i>7<i>c</i> by GC-MS), respectively. Based on the phenotypic and phylogenetic characteristics, strain PL04ᵀ is considered to represent a novel species of the genus <i>Formosa</i>, for which the name <i>Formosa sejongensis</i> sp. nov. is proposed, with PL04ᵀ (=KCTC 102051ᵀ=DSM 117045ᵀ) as the type strain. Similarly, strain SM01ᵀ is considered to represent a novel species of the genus <i>Polaromonas</i>, for which the name <i>Polaromonas potterensis</i> sp. nov. is proposed, with SM01ᵀ (=KCTC 8096ᵀ=DSM 116566ᵀ) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13148724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nanosynbacter lyticus</i> gen. nov., sp. nov., an ultrasmall, obligate epibiont bacterium and the proposal of <i>Nanosynbacteraceae</i> fam. nov., <i>Nanosynbacterales</i> ord. nov. and <i>Nanosynbacteria</i> class nov.","authors":"Erik L Hendrickson, Batbileg Bor, Xuesong He, Jeffrey S McLean","doi":"10.1099/ijsem.0.007162","DOIUrl":"https://doi.org/10.1099/ijsem.0.007162","url":null,"abstract":"<p><p>The recently validly published phylum <i>Minisyncoccota</i> (previously <i>Ca</i>. Patescibacteriota or candidate phyla radiation) had long been an enigma, with members having remained uncultivated for several decades since the first SSU ribosomal sequence was recovered. We reported on the first isolated strain of <i>Minisyncoccota</i>, <i>Nanosynbacter lyticus</i> type strain TM7x^T (ATCC TSD-290^T), in 2015, using directed cultivation from human oral samples. Strain TM7x^T had an extremely reduced genome (705 CDS), ultra-small cell size (200-300 nm), and replicated as an obligate epibiont on the surface of another commonly found oral species: <i>Pauljensenia odontolytica_C</i> strain XH001 (host bacterium). TM7x^T also exhibited what has been defined as a parasitic phase, where it disrupts the membrane of the bacterial host cell, resulting in host bacteria lysis. However, free-floating, host-free TM7x^T cells remain viable and can re-infect fresh host bacterial cells when available. This discovery and coculture marked the first concrete evidence of how these ultrasmall organisms within the <i>Minisyncoccota</i> survive and persist despite their reduced genomes missing <i>de novo</i> biosynthesis of many essential compounds, including all amino acids and vitamins. This model dual species system has now allowed a deeper understanding of the TM7x-host bacterium interaction and dynamics, with over 15 publications reporting on various aspects of this strain to date. We propose the name <i>Nanosynbacter lyticus</i> gen. nov. sp. nov. for strain TM7x^T. We also propose <i>Nanosynbacteraceae</i> fam. nov., <i>Nanosynbacterales</i> ord. nov. and <i>Nanosynbacteria</i> class nov. to accommodate the genus <i>Nanosynbacter</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptomyces mabaensis</i> sp. nov., isolated from karst cave samples of ancient Ma'ba remains.","authors":"Ting-Ting She, Bao-Zhu Fang, Xin-Yao Li, Yi-Wen Liang, Ze-Xian Wu, Zi-Tong Zhang, Shuai Li, Wen-Jun Li","doi":"10.1099/ijsem.0.007153","DOIUrl":"https://doi.org/10.1099/ijsem.0.007153","url":null,"abstract":"<p><p>Caves are generally considered to be natural subterranean spaces with nutrient-deficient conditions and represent a particular source of uncharacterized microbial diversity. During a culturable actinomycetes diversity research, 84 <i>Streptomyces</i> spp. strains were harvested from the karst cave of the Maba hominins (one of the most important representatives of archaic <i>Homo sapiens</i> in China) in Shaoguan City. Among these culturable <i>Streptomyces</i> strains, two novel strains, SYSU K21746^T and SYSU K217416, were identified and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strains SYSU K21746^T and SYSU K217416 were closely related to <i>Streptomyces hiroshimensis</i> NBRC 12785^T (98.2%/98.1% sequence similarity), <i>Streptomyces abikoensis</i> NBRC 13860^T (98.1%/98.0%) and <i>Streptomyces genisteinicus</i> CRPJ-33^T (98.0%/97.9%). The two strains showed nearly identical 16S rRNA gene sequences (99.5%), and the average nucleotide identity and digital DNA-DNA hybridization relatedness values were 98.6% and 85.8%, respectively, suggesting that these strains are affiliated with the same species. Furthermore, the major polar lipids of the two strains were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The MK-9(H₈) and MK-9(H₆) were the main isoprenoid quinones of the two strains. The major fatty acids of the two strains were iso-C_16:0 and anteiso-C_15:0. In addition, the chemotaxonomic and culture characteristics of both strains were also consistent with the genus <i>Streptomyces</i>, while phenotypic properties, genome-based comparisons and phylogenomic analyses distinguished strains SYSU K21746^T and SYSU K217416 from their closest phylogenetic relatives. According to these results, two isolates from the karst cave niche represent a novel species of the genus <i>Streptomyces</i>, for which the name <i>Streptomyces mabaensis</i> sp. nov. is proposed, with SYSU 21746^T (=NBRC 115001^T=CGMCC 4.7690^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tongtianella jiaweipingae</i> gen. nov., sp. nov. and <i>Schaalia zhouxuedongae</i> sp. nov., two new members of the family <i>Actinomycetaceae</i>, isolated from the respiratory tract of <i>Marmota himalayana</i> in the Qinghai-Tibet Plateau.","authors":"Wenbo Luo, Yuanmeihui Tao, Juan Zhou, Gui Zhang, Mengshan Chen, Yuqing Liu, Ji Pu, Han Zheng, Jing Yang, Jianguo Xu","doi":"10.1099/ijsem.0.007142","DOIUrl":"https://doi.org/10.1099/ijsem.0.007142","url":null,"abstract":"<p><p>Four Gram-stain-positive, rod-shaped and non-spore-forming strains zg-332^T, ZJ293, ZJ405^T and ZJ1691 were isolated from the respiratory tract of <i>Marmota himalayana</i> in the Qinghai-Tibet Plateau of China. Comparative 16S rRNA gene sequence analysis revealed that strain zg-332^T shares 99.6% sequence similarity with strain ZJ293 and is most similar to strain <i>Pauljensenia hongkongensis</i> DSM 15629^T. In contrast, strains ZJ405^T and ZJ1691 share 99.7% similarity with each other and are most closely related to strain <i>Schaalia vaccimaxillae</i> DSM 15804^T and strain <i>Schaalia cardiffensis</i> CCUG 44997^T. Phylogenetic analyses based on 16S rRNA gene sequences confirmed these groupings. The G+C content of strains zg-332^T and ZJ405^T is 35.4 mol% and 57.9 mol%, respectively. Comparative genomic analysis indicated that strains zg-332^T and ZJ293 represent a novel genus in the family <i>Actinomycetaceae</i>, whereas strains ZJ405^T and ZJ1691 represent a novel species within the genus <i>Schaalia</i>. Optimal growth occurred at 35.0 °C, pH 8.0 with 0.5% NaCl (w/v) for strain zg-332^T, and 37.0 °C, pH 8.0 with 0.5-1.5% NaCl (w/v) for strain ZJ405^T. The major fatty acids are C_{16 : 0} and C_{18 : 1} <i>ω</i>9<i>c</i> for both strains zg-332^T and ZJ405^T. The polar lipids for both strains include diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unidentified glycolipids, with additional phosphatidyl inositol mannosides, unidentified phospholipid for strain zg-332^T and unidentified lipids for strain ZJ405^T. The respiratory quinone of strain zg-332^T is MK-9(H₂) and MK-9(H₄), and that of strain ZJ405^T is MK-10(H₄). The peptidoglycan of both strains zg-332^T and ZJ405^T contains alanine, glutamic acid, glycine and lysine. Based on the polyphasic analyses, strains zg-332^T (=KACC 22128^T=GDMCC 1.1729^T) and ZJ293 represent a novel genus and species within the family <i>Actinomycetaceae</i>, for which the name <i>Tongtianella jiaweipingae</i> gen. nov., sp. nov. is proposed; strains ZJ405^T (=GDMCC 1.1953^T=KACC 22129^T) and ZJ1691 represent a novel species of the genus <i>Schaalia</i>, for which the name <i>Schaalia zhouxuedongae</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Thermus javaensis</i> sp. nov., a novel thermophilic bacterium isolated from litter of a geyser in Cisolok, West Java, Indonesia.","authors":"Fitria Ningsih, Mazytha Kinanti Rachmania, Dhian Chitra Ayu Fitria Sari, Yasunori Ichihashi, Song-Gun Kim, Shuhei Yabe, Wellyzar Sjamsuridzal","doi":"10.1099/ijsem.0.007136","DOIUrl":"https://doi.org/10.1099/ijsem.0.007136","url":null,"abstract":"<p><p>A thermophilic, yellow-pigmented bacterial strain, designated LT1-2-5^T, was isolated from litter in the Cisolok Geyser, West Java, Indonesia. This strain is Gram-stain-negative, aerobic and non-sporulating, with rod-shaped cells and short filaments. Phylogenetic analyses based on 16S rRNA gene sequences (1,446 bp) placed strain LT1-2-5^T within the genus <i>Thermus</i>, showing highest similarities to <i>Thermus thalpophilus</i> SYSU G00506^T (98.54%) and <i>Thermus brockianus</i> YS038^T (98.34%). The strain exhibited optimal growth at 60-65 °C, pH 7.0-8.0 and ≤2.0% (w/v) sodium chloride (NaCl). Major cellular fatty acids (>10%) were iso-C_17:0, iso-C_15:0, anteiso-C_15:0 and anteiso-C_17:0. The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified glycolipids. The major menaquinone was menaquinone-8. The genome size of strain LT1-2-5^T was 2.41 Mbp, and the DNA G+C content was 66.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain LT1-2-5^T and <i>T. thalpophilus</i> SYSU G00506^T were 91.46% and 42.9%, respectively, both of which are below the established species cut-off value. Polyphasic taxonomy, including phylogenetic, genomic, phenotypic and chemotaxonomic analyses, confirmed the status of strain LT1-2-5^T as a novel species of <i>Thermus</i>, for which the name <i>Thermus javaensis</i> sp. nov. is proposed. The type strain is LT1-2-5^T (=UICC B-84^T=CGMCC 1.61921^T=KCTC 102310^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13142892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paucilactobacillus salsurae</i> sp. nov. and <i>Enterococcus salsurae</i> sp. nov., isolated from traditional Chinese pickle.","authors":"Si-Tong Song, Jing-Hao Wang, Chaoxin Man, Chun Tao Gu","doi":"10.1099/ijsem.0.007152","DOIUrl":"https://doi.org/10.1099/ijsem.0.007152","url":null,"abstract":"<p><p>Three novel bacteria (designated as N302-9^T, N342-3-1-2^T and N249-2) were isolated from traditional Chinese pickle ('Suan cai') and identified. Strain N302-9^T was phylogenetically related to the type strains of <i>Paucilactobacillus wasatchensis</i>, <i>Paucilactobacillus kaifaensis</i> and <i>Paucilactobacillus hokkaidonensis</i>. Strains N342-3-1-2^T and N249-2 were phylogenetically related to the type strains of <i>Enterococcus gallinarum</i>, <i>Enterococcus innesii</i> and <i>Enterococcus casseliflavus</i>. Strain N302-9^T had 71.4-72.5% ANI and 18.7-19.9% dDDH values with the type strains of all <i>Paucilactobacillus</i> species. Strains N342-3-1-2^T and N249-2 shared 99.3% ANI and 94.3% dDDH values. Strain N342-3-1-2^T had 75.2-77.0% ANI and 20.5-21.1% dDDH values with the type strains of <i>E. gallinarum</i>, <i>E. innesii</i> and <i>E. casseliflavus</i>. Based upon the data obtained in the present study, two novel species, <i>Paucilactobacillus salsurae</i> sp. nov. and <i>Enterococcus salsurae</i> sp. nov., are proposed, and the type strains are N302-9^T (=JCM 37828^T=GDMCC 1.5980^T) and N342-3-1-2^T (=JCM 37829^T=LMG 33889^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147837846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Daquilactobacillus linshuiensis</i> gen. nov., sp. nov., a novel lactic acid bacterium isolated from Nongxiangxing Baijiu Daqu.","authors":"Xin Huang, Jie Liu, Lingling Yang, Kaizheng Zhang, Youqiang Xu, Wei Zou","doi":"10.1099/ijsem.0.007157","DOIUrl":"https://doi.org/10.1099/ijsem.0.007157","url":null,"abstract":"<p><p>A facultatively anaerobic, Gram-stain-positive, non-spore-forming, non-motile, short-rod-shaped lactic acid bacterium, designated strain DQM5ᵀ, was isolated from Nongxiangxing Baijiu Daqu, a traditional Chinese fermentation starter. On the basis of 16S rRNA gene sequence similarity, strain DQM5ᵀ exhibited the highest similarity to <i>Companilactobacillus mishanensis</i> (93.87%). In the 16S rRNA gene-based phylogenetic tree, strain DQM5ᵀ formed a distinct lineage located between the <i>Companilactobacillus</i> and <i>Latilactobacillus</i> clades. Phylogenomic analysis revealed that the average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between strain DQM5ᵀ and its most closely related taxa were at most 58.6%, 70.1% and 26.5%, respectively, which are well below the thresholds for genus and species delineation. Notably, the genome size (1.40 Mbp) and DNA G+C content (30.8 mol%) of strain DQM5ᵀ are significantly different from those of related taxa. The peptidoglycan contains lysine as the diagnostic diamino acid. The major cellular fatty acids are C_₁₈:₁ ω9c and C_₁₆:₀. These distinctive genomic features, together with other phenotypic, chemotaxonomic and phylogenetic differences, provide strong evidence for proposing a completely new genus. Therefore, strain DQM5ᵀ represents a novel species of a new genus within the family <i>Lactobacillaceae</i>, for which the name <i>Daquilactobacillus linshuiensis</i> gen. nov., sp. nov. is proposed. The type strain is DQM5ᵀ (=GDMCC 1.5776ᵀ=JCM 38144ᵀ).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}