International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Tersicoccus mangrovi</i> sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia.","authors":"Mada Triandala Sibero, Zhou-Qing Zheng, Fei-Na Li, Shao-Wei Liu, Xin Xiang, Li Tuo, Cheng-Hang Sun","doi":"10.1099/ijsem.0.006669","DOIUrl":"https://doi.org/10.1099/ijsem.0.006669","url":null,"abstract":"<p><p>A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9^T, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9^T grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9^T was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9^T formed a well-supported clade with <i>Tersicoccus solisilvae</i> CGMCC 1.15480^T and <i>Tersicoccus phoenicis</i> 1P05MA^T and shared the highest similarity to <i>T. solisilvae</i> CGMCC 1.15480^T (98.9% sequence similarity) and <i>T. phoenicis</i> 1P05MA^T (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9^T and <i>T. solisilvae</i> CGMCC 1.15480^T gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C_15:0, anteiso-C_17:0 and iso-C_16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H₂) and MK-9(H₂) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9^T represents a novel species of the genus <i>Tersicoccus</i>. The name <i>Tersicoccus mangrovi</i> sp. nov. is proposed, with the type strain MR15.9^T (=MCCC 1K08875^T=KCTC 59105^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Committee on Systematics of Prokaryotes: minutes of the open plenary meeting, Tuesday, 22 October 2024, Sesto Fiorentino, Florence, Italy, and via Teams.","authors":"Aharon Oren","doi":"10.1099/ijsem.0.006621","DOIUrl":"10.1099/ijsem.0.006621","url":null,"abstract":"<p><p>A hybrid in-person and online open plenary meeting of the International Committee on Systematics of Prokaryotes (ICSP) was held on 22 October 2024 at the Consiglio Nazionale delle Ricerche, Sesto Fiorentino, Florence, Italy, and via Teams just prior to the IUMS 2024 Congress. To comply with Articles 4(d) and 5(d) (1) of the statutes of the ICSP, the minutes of this meeting are published here.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based reclassification of <i>Suonthocola fibrivorans</i> as a later heterotypic synonym of <i>Diplocloster agilis</i>.","authors":"Yu Chyuan Heng","doi":"10.1099/ijsem.0.006616","DOIUrl":"10.1099/ijsem.0.006616","url":null,"abstract":"<p><p>Both the genera <i>Suonthocola</i> and <i>Diplocloster</i> are members of the family <i>Lachnospiraceae</i>. Their type species, both <i>Suonthocola fibrivorans</i> Sanger_33^T and <i>Diplocloster agilis</i> ASD5720^T, were isolated from human faeces. A comparison of their 16S rRNA gene sequences revealed 100% similarity, suggesting their close relatedness and the possibility of belonging to the same species. To clarify their taxonomic relationship, genome-based analyses were carried out. Overall genomic relatedness indices analyses indicated that <i>S. fibrivorans</i> Sanger_33^T shared average amino acid identity and percentage of conserved proteins prediction values higher than the <i>Lachnospiraceae</i>-specific genus-level thresholds with <i>D. agilis</i> ASD5720^T, as well as the other two existing species of the genus <i>Diplocloster</i>. Additionally, <i>S. fibrivorans</i> Sanger_33^T formed a distinct branch with <i>D. agilis</i> ASD5720^T, clustering with the other two species of the genus <i>Diplocloster</i> into the same clade in both the 16S rRNA gene phylogenetic tree and the core-genome phylogenomic tree. Essentially, both the average nucleotide identity and digital DNA-DNA hybridization prediction values between <i>S. fibrivorans</i> Sanger_33^T and <i>D. agilis</i> ASD5720^T were above the recommended species boundaries. It is thus clear that <i>S. fibrivorans</i> Sanger_33^T and <i>D. agilis</i> ASD5720^T constitute the same species. On the basis of the earliest valid publication, priority is given to <i>D. agilis</i> Chaplin <i>et al</i>. 2022. Based on this, the orphan species <i>S. fibrivorans</i> Hitch <i>et al</i>. 2022 is reclassified as a later heterotypic synonym of <i>D. agilis</i> Chaplin <i>et al</i>. 2022, along with the reclassification of the genus <i>Suonthocola</i> Hitch <i>et al</i>. 2022 as a later synonym of <i>Diplocloster</i> Chaplin <i>et al</i>. 2022.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the nomenclatural status of the prokaryotic name <i>Skermania piniformis</i> (Blackall <i>et al.</i> 1989) Chun <i>et al</i>. 1997. Request for an Opinion.","authors":"Meng-Syun Li","doi":"10.1099/ijsem.0.006659","DOIUrl":"10.1099/ijsem.0.006659","url":null,"abstract":"<p><p>In 1997, the name <i>Skermania piniformis</i> (Blackall <i>et al.</i> 1989) comb. nov. was proposed by Chun <i>et al.</i> on transfer of the species <i>Nocardia pinensis</i> to the newly established genus <i>Skermania</i> as its type species. The appearance of the epithet in <i>S. piniformis</i> was quite different from that in its basonym <i>N. pinensis.</i> This could be seen, at first glance, as an unnecessary epithet change that may render <i>S. piniformis</i> and its generic name <i>Skermania</i> illegitimate according to the International Code of Nomenclature of Prokaryotes (ICNP). But in this paper, the author argues that for this particular case, '<i>pinensis</i>' and '<i>piniformis</i>' are better treated as different spellings of the same epithet. And if so, the change from '<i>pinensis</i>' to '<i>piniformis</i>' will not give rise to a case of illegitimacy under Rule 41a of the ICNP. Several possible counterarguments are discussed, which are shown to be based on a misinterpretation of the ICNP, particularly in relation to Rule 20a. As this is a non-trivial issue, the author requests a final decision by the Judicial Commission according to Rule 58 of the ICNP. An additional request for clarifying Rule 54 is also made.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of six novel species <i>Bacillus bruguierae</i> sp. nov., <i>Bacillus kandeliae</i> sp. nov., <i>Bacillus yunxiaonensis</i> sp. nov., <i>Metabacillus rhizosphaerae</i> sp. nov., <i>Metabacillus sediminis</i> sp. nov. and <i>Psychrobacillus mangrovi</i> sp. nov., isolated from mangrove ecosystem.","authors":"Guo-Hong Liu, Manik Prabhu Narsing Rao, Huai Shi, Qian-Qian Chen, Syed Raziuddin Quadri, Wen-Jun Li","doi":"10.1099/ijsem.0.006656","DOIUrl":"10.1099/ijsem.0.006656","url":null,"abstract":"<p><p>Six Gram-stain-positive and rod-shaped strains, designated FJAT-51614^T, FJAT-51639^T, FJAT-52054^T, FJAT-52991^T, FJAT-53654^T and FJAT-53711^T, were isolated from a mangrove ecosystem. The condition for growth among the strains varied (pH ranging 5.0-11.0 and temperature 15-45 °C). These strains showed the highest 16S rRNA gene sequence similarity to the genera <i>Bacillus</i>, <i>Metabacillus</i> and <i>Psychrobacillus</i>. Menaquinone MK-7 (100%) was present in all strains except strain FJAT-51614^T, which contained MK-7 (32%) and MK-8 (68%). The major fatty acids (>10%) in strains FJAT-52054^T and FJAT-51614^T were anteiso-C_15 : 0 and iso-C_14 : 0. The major fatty acid (>10%) in strain FJAT-53654^T was anteiso-C_15 : 0, while in strain FJAT-51639^T were iso-C_15 : 0 and iso-C_17 : 0. The major fatty acids in strains FJAT-52991^T and FJAT-53711^T were iso-C_15 : 0 and iso-C_16 : 0. The major polar lipids in strains FJAT-51639^T, FJAT-52991^T and FJAT-51614^T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, while strains FJAT-53711^T, FJAT-52054^T and FJAT-53654^T consist of diphosphatidylglycerol and phosphatidylglycerol. The genome-relatedness values among the present study strains and the closest species were below the recognized cutoff levels for species delineation. The polyphasic analysis comparison results represent six strains as novel species for which the names <i>Bacillus bruguierae</i> sp. nov. (FJAT-51639^T=GDMCC 1.3073^T=JCM 35615^T), <i>Bacillus kandeliae</i> sp. nov. (FJAT-52991^T=GDMCC 1.3069^T=JCM 35619^T), <i>Bacillus yunxiaonensis</i> sp. nov. (FJAT-53711^T=GDMCC 1.3068^T=JCM 35616^T), <i>Metabacillus rhizosphaerae</i> sp. nov. (FJAT-53654^T=GDMCC 1.3075^T=JCM 35614^T), <i>Metabacillus sediminis</i> sp. nov. (FJAT-52054^T=GDMCC 1.3074^T=JCM 35620^T) and <i>Psychrobacillus mangrovi</i> sp. nov. (FJAT-51614^T=GDMCC 1.3072^T=JCM35478^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Flavobacterium arundinis</i> sp. nov., <i>Flavobacterium calami</i> sp. nov., <i>Flavobacterium helocola</i> sp. nov. and <i>Flavobacterium flavipallidum</i> sp. nov., isolated from stagnant water in a clump of <i>Phragmites japonica</i> Steud.","authors":"Sunho Park, Inhyup Kim, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo","doi":"10.1099/ijsem.0.006667","DOIUrl":"https://doi.org/10.1099/ijsem.0.006667","url":null,"abstract":"<p><p>Four novel bacterial strains were isolated from stagnant water in a clump of <i>Phragmites japonica</i> Steud. The four Gram-negative, facultatively anaerobic, rod-shaped, non-motile and yellow-coloured strains were designated as DGU11^T, DGU38^T, DGU41^T and DGU99^T. The four novel strains exhibited 16S rRNA gene similarities with their closest type strains, ranging from 95.7 to 98.7%, average nucleotide identity values from 75.3 to 95.6% and digital DNA-DNA hybridization values between 17.9 and 59.6%. The cells of strain DGU11^T were non-motile and grew at 10-30°C (optimum 25 °C), a pH range of 4.0-12.0 (optimum 9.0) and in the presence of 0-1.0% sodium chloride (NaCl) (optimum 0%). The cells of strain DGU38^T were non-motile and grew at 10-35°C (optimum 30 °C), a pH range of 4.0-8.0 (optimum 7.0) and at 0 -2.0% NaCl (optimum 0%). The cells of strain DGU41^T were non-motile and grew at 15-50°C (optimum 30 °C), a pH range of 6.0-9.0 (optimum 7.0) and in the presence of 0% NaCl (optimum 0%). The cells of strain DGU99^T were non-motile and grew at 15-35°C (optimum 25 °C), a pH range of 6.0-12.0 (optimum 9.0) and in the presence of 0 -2.0% NaCl (optimum 0%). The major fatty acid composition of the four novel strains was iso-C_15:0 and C_16:1 <i> ω7</i>c<i>/</i>C1_6:1 <i> ω6</i>c, which is similar to other <i>Flavobacterium</i> species. The proposed names of the novel strains are <i>Flavobacterium arundinis</i> sp. nov. (type strain DGU11^T=KACC 23722^T=TBRC 19004^T), <i>Flavobacterium calami</i> sp. nov. (type strain DGU38^T=KACC 23723^T=TBRC 19005^T), <i>Flavobacterium helocola</i> sp. nov. (type strain DGU41^T=KACC 23724^T=TBRC 19006^T) and <i>Flavobacterium flavipallidum</i> sp. nov. (type strain DGU99^T=KACC 23725^T=TBRC 19007^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudobensingtonia carpini</i> sp. nov., a novel yeast species isolated from plant leaves in China.","authors":"Chun-Yue Chai, Bing-Yan Song, Dan Lu, Cai-Ying Zhang, Feng-Li Hui","doi":"10.1099/ijsem.0.006642","DOIUrl":"https://doi.org/10.1099/ijsem.0.006642","url":null,"abstract":"<p><p>Two novel yeast strains, NYNU 236247 and NYNU 23523, were isolated from the leaves of <i>Carpinus turczaninowii</i> Hance, collected in the Tianchi Mountain National Forest Park, Henan Province, central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region revealed the closest relatives of the strains are three described <i>Pseudobensingtonia</i> species: <i>Ps. fusiformis</i>, <i>Ps. musae</i> and <i>Ps. ingoldii</i>. The novel species differed from the type strains of these three species by 12 to 22 nucleotide substitutions and 1 gap (~2.0-4.0%) in the D1/D2 domain and by 78 to 100 nucleotide mismatches (~12.0-16%) in the ITS region. Physiologically, the novel species differs from <i>Ps. fusiformis</i> and <i>Ps. musae</i> in its ability to assimilate dl-lactate and melezitose and from <i>Ps. ingoldii</i> by its inability to assimilate melibiose, soluble starch and ethanol. <i>Pseudobensingtonia carpini</i> sp. nov. is proposed for those two strains, with the holotype designated as GDMCC 2.483^T (MycoBank MB 857072).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudomonas retamae</i> sp. nov., a novel endophytic bacterium with plant growth-promoting potential, isolated from root nodules of <i>Retama monosperma</i> in Northwestern Algeria.","authors":"Nawel Selami, Fatima El-Houaria Zitouni-Haouar, Chahira Zerouki, Chahrazed Aibeche, Nassima Draou, Omar Khelil, Slimane Choubane, Makaoui Maatallah, Ikram Madani, Khadidja Ouenzar, Assia Zemmour, Mohamed Kerkoud, Bacem Mnasri, Bouziane Moumen, Cécile Vriet, Abderrezak Djabeur","doi":"10.1099/ijsem.0.006646","DOIUrl":"https://doi.org/10.1099/ijsem.0.006646","url":null,"abstract":"<p><p>A thorough polyphasic taxonomic study, integrating genome-based taxonomic approaches, was carried out to characterize the RB5^T strain isolated from root nodules of <i>Retama monosperma</i> growing on the coastal dunes of Bousfer Beach (Oran, Algeria). The 16S rRNA gene sequence analysis revealed that strain RB5^T had the highest similarity to <i>Pseudomonas granadensis</i> LMG27940^T (98.94%) and <i>Pseudomonas gozinkensis</i> IzPS32d^T (98.73%). Phylogenetic studies, including both 16S rRNA gene sequence and multilocus sequence analysis using 16S <i>rRNA</i>, <i>gyrB</i> and <i>rpoD</i> housekeeping genes, positioned RB5^T in a distinct branch alongside its closest relative, <i>P. granadensis</i> LMG27940^T. Phylogenomic analysis using the Bac120 marker set and Type (Strain) Genome Server confirmed the unique position of RB5^T and its close relationship with <i>P. granadensis</i> LMG27940^T. Similarly, genomic comparisons using average nucleotide identity based on blast (ANIb) and digital DNA-DNA hybridization (dDDH) revealed values of 92.85 and 59.3%, respectively, when compared with its closest relative, <i>P. granadensis</i> LMG27940^T. Both values fall below the established species delimitation thresholds of 95-96% for ANIb and 70% for dDDH, providing strong genomic evidence that strain RB5^T represents a novel species. Further average nucleotide identity comparisons with unclassified <i>Pseudomonas</i> spp. (384 genomes) and metagenomic-derived genomes from the Genome Taxonomy Database (GTDB) showed values between 84.27 and 89.2%, indicating that strain RB5^T belongs to a unique evolutionary line. The genome of RB5^T, with a size of 6 311 310 bp and a G+C content of 60%, harbours several key genes associated with plant growth-promoting traits, making it a promising candidate for sustainable agriculture. Phenotypically, RB5^T strain is an aerobic, rod-shaped, Gram-negative, non-spore-forming bacterium that is motile with a single polar flagellum. It grows under a wide range of temperature (4-42 °C) and pH (5-10) conditions and tolerates up to 6% (w/v) NaCl. The main cellular fatty acid composition of RB5^T includes C_16:0, C_17:0 cyclo and the summed features 3 consisting of C_16:1 ω7c/_C16:1 ω6c. Based on the phylogenetic, phenotypic, chemotaxonomic and genome comparison analyses, strain RB5^T was identified as a novel species of the genus <i>Pseudomonas</i>, for which the name <i>Pseudomonas retamae</i> sp. nov. is proposed. The type strain is RB5^T (=DSM 117471^T=LMG 33633^T=CIP 112482^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Veillonella absiana</i> sp. nov., isolated from pig faeces.","authors":"Lu Bai, Jayoung Paek, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang","doi":"10.1099/ijsem.0.006643","DOIUrl":"https://doi.org/10.1099/ijsem.0.006643","url":null,"abstract":"<p><p>Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232^T and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to <i>Veillonella rogosae</i> KCTC 5967^T, with 97.0% similarity. Analysis of housekeeping gene sequences (<i>rpoB</i>) revealed the strain formed a sub-cluster within the genus <i>Veillonella</i>. The average nucleotide identities between the two isolates and the most closely related strains within genus <i>Veillonella</i> were <75.0%. The major fatty acids were Summed Feature 8 and C_16 : 1 <i> ω9</i>c. The cell wall peptidoglycan contained <i>meso</i>-diaminopimelic acid. The major metabolic end products of isolates were propionic and acetic acids. The genomic DNA G+C contents of both strains were 40.1 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-vei2232^T (=KCTC 25748^T=NBRC 116427^T) and YH-vei2233 (=KCTC 25749=NBRC 116428) suggest that they represent a novel species of the genus <i>Veillonella</i>, for which the name <i>Veillonella absiana</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tenacibaculum platacis</i> sp. nov., <i>Tenacibaculum vairaonense</i> sp. nov. and <i>Tenacibaculum polynesiense</i> sp. nov. isolated from batfish (<i>Platax orbicularis</i>) in Tahiti Island, French Polynesia.","authors":"Pierre Lopez, Benjamin Fradet, Lucie Coffion, Jean-François Bernardet, Denis Saulnier, Eric Duchaud","doi":"10.1099/ijsem.0.006605","DOIUrl":"https://doi.org/10.1099/ijsem.0.006605","url":null,"abstract":"<p><p>Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05c^T, 190524A02b^T and 190130A14a^T were retrieved from orbicular batfish (<i>Platax orbicularis</i>) mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus <i>Tenacibaculum</i>. Phylogenetic analyses based on 679 concatenated core protein sequences revealed that strains 190524A05c^T, 190524A02b^T and 190130A14a^T showed the highest similarity to <i>Tenacibaculum skagerrakense</i> DSM 14836^T, <i>Tenacibaculum xiamenense</i> LMG 27422^T and <i>Tenacibaculum holothuriorum</i> S2-2^T, respectively. Digital DNA-DNA hybridization and average nt identity values between strains 190524A05c^T, 190524A02b^T and 190130A14a^T and other type strains were less than 76.25 and 24.1%, respectively. The DNA G+C content was 31.48, 30.66 and 31.98 mol% for strains 190524A05c^T, 190524A02b^T and 190130A14a^T, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in these three strains. The major polar lipids (phosphatidylethanolamine and aminophospholipid) were similar to the chemotaxonomic profile of other species of the genus <i>Tenacibaculum</i>. Strain 190524A05c^T contained summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:1 G, iso-C_15:0 and iso-C_17:0 3-OH as the major cellular fatty acids. Strain 190524A02b^T contained summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:0, iso-C_15:1 G and iso-C_17:0 3-OH as the major cellular fatty acids. Strain 190130A14a^T contained iso-C_15:1 G, summed feature 3 (comprising C_16:1 ω7c and/or iso-C_15:0 2-OH), iso-C_15:0 and iso-C_17:0 3-OH as the major cellular fatty acids. Based on the phenotypic and molecular features, these three strains represent novel species of the genus <i>Tenacibaculum</i> for which the names <i>Tenacibaculum platacis</i> sp. nov., with 190524A05c^T (= CIP 112470^T = DSM 118113^T) as the type strain; <i>Tenacibaculum vairaonense</i> sp. nov., with 190524A02b^T (= CIP 112469^T = DSM 118112^T) as the type strain; and <i>Tenacibaculum polynesiense</i> sp. nov., with 190130A14a^T (= CIP 112468^T = DSM 118111^T) as the type strain, are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}