International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Microbispora maris</i> sp. nov., a novel actinobacterium isolated from the gill of the leopard coral grouper (<i>Plectropomus leopardus</i>).","authors":"Huan Xie, Qing-Yi Xie, Qing-Yun Ma, Li Yang, Hao-Fu Dai, You-Xing Zhao, Yu-E Hao","doi":"10.1099/ijsem.0.006568","DOIUrl":"https://doi.org/10.1099/ijsem.0.006568","url":null,"abstract":"<p><p>A novel actinomycete, designated strain ZYX-F-249^T, was isolated from the gill of a leopard coral grouper in Yongxing Island, Hainan Province, China. Based on 16S rRNA gene sequence analysis, strain ZYX-F-249^T belonged to the genus <i>Microbispora</i>, with high similarities to <i>Microbispora rosea</i> ATCC 12950^T (98.7 %), <i>Microbispora hainanensis</i> 211020^T (98.5 %), <i>Microbispora clausenae</i> CLES2^T (98.4 %), <i>Microbispora bryophytorum</i> NEAU-TX2-2^T (98.1 %) and <i>Microbispora oryzae</i>RL4-1S^T (98.0 %). Phylogenetic analysis of the 16S rRNA gene sequence of strain ZYX-F-249^T showed that the strain formed a stable subclade with <i>Microbispora rosea</i> ATCC 12950^T in the genus <i>Microbispora</i>. The cell wall of the novel isolate contained <i>meso</i>-diaminopimelic acid, while whole-cell sugars were madurose, glucose and ribose. The predominant menaquinones were MK-9(H₆), MK-9(H₄), MK-9(H₂) and MK-9(H₀). The characteristic phospholipids (PLs) were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, glycophospholipids and an unknown PL. The major cellular fatty acids (>10%) were <i>iso</i>-C_16 : 0 and <i>iso</i>-C_18 : 0. Genome sequencing showed a DNA G+C content of 71.6%. The low average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values demonstrated that strain ZYX-F-249^T could be readily distinguished from closely related species. Based on data from these phylogenetic, chemotaxonomic and physiological characteristics, strain ZYX-F-249^T represents a novel species of the genus <i>Microbispora</i>, for which the name <i>Microbispora maris</i> sp. nov. is proposed. The type strain is ZYX-F-249^T (=CCTCC AA 2023030^T =JCM 36778^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International Committee on Systematics of Prokaryotes: subcommittee on the taxonomy of methanogenic archaea. Minutes of the closed, online meeting held 06 September 2023.","authors":"Jeremy A Dodsworth, Om Prakash","doi":"10.1099/ijsem.0.006569","DOIUrl":"https://doi.org/10.1099/ijsem.0.006569","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations, and new taxonomic opinions have appeared in volume 74, part 7 of the IJSEM.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006494","DOIUrl":"https://doi.org/10.1099/ijsem.0.006494","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum. Proposal of <i>Lactobacillus amylovorus</i> subsp. <i>animalis</i> subsp. nov. and an emended description of <i>Lactobacillus amylovorus</i>.","authors":"Kenji Yamane, Yasuhiro Tanizawa, Hisami Kobayashi, Tomomi Kamizono, Yoichiro Kojima, Hiroki Takagi, Masanori Tohno","doi":"10.1099/ijsem.0.006564","DOIUrl":"https://doi.org/10.1099/ijsem.0.006564","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Bifidobacterium favimelis</i> sp. nov., isolated from black comb honey.","authors":"Yu Li, Qiujie Song, Haizhu Yang, Yue Wei, Bilege Menghe, Wenjun Liu","doi":"10.1099/ijsem.0.006573","DOIUrl":"https://doi.org/10.1099/ijsem.0.006573","url":null,"abstract":"<p><p>A strain of the bacterial genus <i>Bifidobacterium</i>, IMAU50987^T, that was isolated from black comb honey from Lianghe County, Dehong Autonomous Prefecture, Yunnan Province, China, could not be accurately identified as a known species using 16S rRNA gene sequencing. In this study, the taxonomic status of strain IMAU50987^T was evaluated using whole-genome sequencing combined with phenotypic analyses. Strain IMAU50987^T was Gram-positive, non-motile and non-spore-forming. Comparative of 16S rRNA gene sequence similarity analysis indicated that strain IMAU50987^T could be attributed to the genus <i>Bifidobacterium</i> and most closely related to the <i>B. mellis</i> Bin7N^T (98.5% identity). Comparative sequence analysis of eight housekeeping genes (<i>pyrG</i>, <i>thrS</i>, <i>glnA1</i>, <i>recA</i>, <i>tuf</i>, <i>dnaB</i>, <i>rpoC</i> and <i>xfp</i>) showed that strain IMAU50987^T was different from closely related species. Phylogenomic analysis based on the 88 single-copy genes in the phylogenetic tree showed that strain IMAU50987^T belonged to the <i>B. asteroides</i> group. The highest average nucleotide identity value for strain IMAU50987^T was 77.0% in comparison with <i>B. choladohabitans</i> B14384H11^T and <i>B. apis</i> F753-1^T. However, the highest digital DNA-DNA hybridization value was 23.1% compared with <i>B. apis</i> F753-1^T and <i>B. xylocopae</i> XV2^T; the highest average amino acid identity value was 73.2% compared with <i>B. polysaccharolyticum</i> W8117^T. These values are below the accepted threshold for novel species boundaries. The draft genome size of strain IMAU50987^T was 1.9 Mb, and the DNA G+C content was 63.5%. The major cellular fatty acids of strain IMAU50987^T were C_16 : 0, C_18 : 1 <i> ω</i>9c and Sum in Feature 7 (C_19 : 1 <i> ω</i>7c/C_19 : 1 <i> ω</i>6c). The cell-wall peptidoglycan was of the A3α l-Lys-Gly type. Based on data from this study, a novel species, <i>Bifidobacterium favimelis</i> sp. nov., is proposed with the type strain IMAU50987^T (= GDMCC 1.4189^T = JCM 36315^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Naohidea akebiae</i> fa. sp. nov., an anamorphic basidiomycete yeast species.","authors":"Zhi-Wen Xi, Xiao-Xi Wang, Feng-Li Hui","doi":"10.1099/ijsem.0.006567","DOIUrl":"https://doi.org/10.1099/ijsem.0.006567","url":null,"abstract":"<p><p>A novel basidiomycete yeast species represented by strain NYNU 2211328 was isolated from a leaf of <i>Akebia trifoliata</i> collected from the Baotianman Nature Reserve in Henan Province, central China. Phylogenetic analysis of the D1/D2 domain of the large subunit rRNA gene and the internal transcribed spacer (ITS) region suggested that this strain is closely related to <i>Naohidea sebacea</i> CBS 8477, exhibiting the similarity values of 96.5% and 91.3% in the D1/D2 domain and the ITS region, respectively. Physiologically, the novel strain differs from <i>N. sebacea</i> in its ability to assimilate inulin, trehalose and d-arabinose, its inability to assimilate dl-lactate and its incapability of growth at 30 °C. Both phenotypic and phylogenetic analyses indicated that the isolated strain represents a novel species in the genus <i>Naohidea</i>, and the name <i>Naohidea akebiae</i> fa. sp. nov. (holotype: CICC 33584; MycoBank number: MB 855585) is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Vishniacozyma floricola</i> sp. nov., a flower-related tremellomycetous yeast species from Europe.","authors":"Dénes Dlauchy, Sergio Álvarez-Pérez, Andrea Tóbiás, Gábor Péter","doi":"10.1099/ijsem.0.006555","DOIUrl":"https://doi.org/10.1099/ijsem.0.006555","url":null,"abstract":"<p><p>During the course of two independent studies conducted in Hungary and Spain, four conspecific yeast strains were isolated from flowers of different plant species. DNA sequences of two barcoding regions, the D1/D2 domain of the LSU rRNA gene and the internal transcribed spacer (ITS) region (ITS1-5.8S rRNA gene-ITS2), revealed that the four strains represent an undescribed <i>Vishniacozyma</i> (family <i>Bulleribasidiaceae</i>, <i>Basidiomycota</i>) species. In terms of pairwise sequence similarities and according to our phylogenetic analyses of the concatenated DNA sequences of the ITS region and the D1/D2 domain of the LSU rRNA gene, the undescribed species is most closely related to <i>Vishniacozyma melezitolytica</i>, a yeast species of phylloplane origin. The novel species differs from the type strain of <i>V. melezitolytica</i> by 8 substitutions and 3 insertion/deletion (indels) and 11 substitutions and 5 indels along the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. In addition to the DNA sequence divergences, the two species differ in some physiological characters as well. We propose the species <i>Vishniacozyma floricola</i> sp. nov. to accommodate the above-noted strains (holotype, NCAIM Y.02320; isotype, CBS 18939; MycoBank number, 856028).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paracoccus spongiarum</i> sp. nov., isolated from the marine sponge, <i>Phakellia elegans</i>.","authors":"Soo-Bin Kim, Kyung Hyun Kim, Jin-Sook Park","doi":"10.1099/ijsem.0.006566","DOIUrl":"https://doi.org/10.1099/ijsem.0.006566","url":null,"abstract":"<p><p>A facultative anaerobic Gram-negative bacterium, designated as strain 2205BS29-5^T, was isolated from a marine sponge, <i>Phakellia elegans</i>, in Beomseom on Jeju Island, Republic of Korea, and taxonomically characterized. The cells were catalase and oxidase positive, non-motile, coccoid-rod shaped and capable of poly-β-hydroxybutyrate production. Growth was observed at 10-37 °C (optimum, 25 °C) and pH 5.0-8.0 (optimum, pH 7.0), and in the presence of 0-9% NaCl (w/v) (optimum, 3.0-4.0%). The major cellular fatty acids and respiratory quinone were identified as summed feature 8 (C_18 : 1 <i> ω</i>7<i>c</i>/C_18 : 1 <i> ω</i>6<i>c</i>) and Q-10, respectively. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, four phosphoglycolipids, two unidentified amino lipids and eight unidentified lipids. The DNA G+C content was 67.8%. Strain 2205BS29-5^T was most closely represented by <i>Paracoccus amoyensis</i> 11-3^T and <i>P. caeni</i> MJ17^T with 97.8 and 97.5% 16S rRNA gene sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA genes and whole-genome sequences showed that strain 2205BS29-5^T was affiliated with the genus <i>Paracoccus</i>. Genomic analysis showed that strain 2205BS29-5^T could synthesize vitamin B family (folate and cobalamin) and ectoine. The average nucleotide identity and digital DNA-DNA hybridization values between strain 2205BS29-5T and <i>P. amoyensis</i> 11-3^T were 77.1% and 18.8%, respectively, and with <i>P. caeni</i> MJ17^T were 78.4 and 21.2%, respectively. Based on phylogenetic, chemotaxonomic and genome relatedness analyses, strain 2205BS29-5^T represents a novel species of the genus <i>Paracoccus</i>, for which the name <i>Paracoccus spongiarum</i> sp. nov. is proposed. The type strain is 2205BS29-5^T (=LMG 33062^T =KACC 23240^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Adlercreutzia wanghongyangiae</i> sp. nov., and <i>Adlercreutzia shanghongiae</i> sp. nov., two new members of the genus <i>Adlercreutzia</i> isolated from plateau pika (<i>Ochotona curzoniae</i>).","authors":"Xiaorui Liu, Lin Ye, Jing Yang, Caixin Yang, Yuyuan Huang, Ji Pu, Liyun Liu, Huimin Zhou, Shuo Ning, Linglin Cao, Jianguo Xu","doi":"10.1099/ijsem.0.006531","DOIUrl":"https://doi.org/10.1099/ijsem.0.006531","url":null,"abstract":"<p><p>Four anaerobic, Gram-stain-positive, non-motile, non-sporulating rod-shaped bacterial strains (R7^T, R21, R22 and R25^T) were isolated from the intestinal contents of plateau pika (<i>Ochotona curzoniae</i>) collected from the Qinghai-Tibet Plateau, PR China. The four isolates grew at between 25 and 42 °C (optimally at 35-37 °C), and with 0.3-3.3% NaCl (w/v) [optimum, 1.3% (w/v)]. Adding l-arginine to the medium could promote their growth. Strains R7^T and R21 were most closely related to <i>Adlercreutzia caecimuris</i> B7^T (97.48% 16S rRNA gene sequence similarity). Strains R25^T and R22 were most closely related to <i>Adlercreutzia</i> e<i>quolifaciens</i> DSM 19450^T (98.25% 16S rRNA gene sequence similarity). The genome sequences of R7^T and R25^T were 2.89 and 2.90 Mb in size with 63.6 and 62.8 mol% DNA G+C contents, respectively. Phylogenetic analysis based on 16S rRNA gene sequences and core genes revealed that R7^T and R21 were most closely related to <i>A. caecimuris</i> B7^T and <i>Adlercreutzia mucosicola</i> DSM 19490^T, whereas R25^T and R22 were most closely related to <i>A. equolifaciens</i> DSM 19450^T and <i>Adlercreutzia rubneri</i> ResAG-91^T. R7^T, R25^T and the closely related species had average nucleotide identity (ANI) values of 81.9-83.2% as well as digital DNA-DNA hybridisation (dDDH) values between 27.3 and 27.9%, which clearly indicated that they represent two novel species within the genus <i>Adlercreutzia</i>. For R7^T and R25^T, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, and the whole cell sugars included galactose, glucose and ribose. On the basis of these results, we propose that strains R7^T and R25^T represent two novel species of the genus <i>Adlercreutzia,</i> namely <i>Adlercreutzia wanghongyangiae</i> sp. nov. and <i>Adlercreutzia shanghongiae</i> sp. nov., respectively. The type strains are R7^T (=GDMCC 1.4459^T=KCTC 25860^T) and R25^T (=GDMCC 1.4458^T=KCTC 25861^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Sorlinia euscelidii</i> gen. nov., sp. nov., a novel acetic acid bacterium isolated from the leafhopper <i>Euscelidius variegatus</i> (<i>Hemiptera</i>: <i>Cicadellidae</i>).","authors":"Ramona Marasco, Grégoire Michoud, Kholoud A Seferji, Elena Gonella, Elisa Garuglieri, Eleonora Rolli, Alberto Alma, Francesca Mapelli, Sara Borin, Daniele Daffonchio, Elena Crotti","doi":"10.1099/ijsem.0.006544","DOIUrl":"10.1099/ijsem.0.006544","url":null,"abstract":"<p><p>Acetic acid bacteria - belonging to the <i>Acetobacteraceae</i> family - are found in the gut of many sugar-feeding insects. In this study, six strains have been isolated from the hemipteran leafhopper <i>Euscelidius variegatus</i>. While they exhibit high 16S rRNA gene sequence similarities to uncultured members of the <i>Acetobacteraceae</i> family, they could not be unequivocally assigned to any particular type species. Considering the clonality of the six isolates, the EV16P^T strain was used as a representative of this group of isolates. The genome sequence of EV16P^T is composed of a 2.388 Mbp chromosome, with a DNA G+C content of 57 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence and whole-genome multilocus sequence analysis indicate that EV16P^T forms a monophyletic clade with the uncultivated endosymbiont of <i>Diaphorina citri</i>, the <i>Candidatus</i> Kirkpatrickella diaphorinae. Such a phylogenetic clade is positioned between those of <i>Asaia-Swaminathania</i> and <i>Kozakia</i>. The genomic distance metrics based on gene and protein sequences support the proposal that EV16P^T is a new species belonging to a yet-undescribed genus. It is a rod-shaped Gram-stain-negative bacterium, strictly aerobic, non-motile, non-spore-forming, showing optimal growth without salt (NaCl) at 30 °C and pH of 6-7. The major quinone is Q10, and the dominant cellular fatty acids (>10%) are C_18:l <i>ω</i>7c, C_19 : 0 cyclo <i>ω</i>6c, C_16 : 0 and C_19 : 1 2OH. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, along with unidentified aminophospholipids, glycophospholipids, aminolipids and lipids. Based on a polyphasic approach, including phylogenetic, phylogenomic, genome relatedness, phenotypic and chemotaxonomic characterisations, EV16P^T (= KCTC 8296^T, = DSM 117028^T) is proposed as a representative of a novel species in a novel genus with the proposed name <i>Sorlinia euscelidii</i> gen. nov., sp. nov., in honour of Prof. Claudia Sorlini, an Italian environmental microbiologist at the University of Milan who inspired the research on microbial diversity, including symbiosis in plants and animals.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}