International journal of systematic and evolutionary microbiology最新文献

{"title":"Proposal of <i>Allobogoriella</i> gen. nov., <i>Allobogoriella caseilytica</i> comb. nov., <i>Allostella</i> gen. nov., <i>Allostella humosa</i> comb. nov. and <i>Allostella vacuolata</i> comb. nov. as replacement names for the illegitimate prokaryotic names <i>Bogoriella</i>, <i>Bogoriella caseilytica</i>, <i>Stella</i>, <i>Stella humosa</i> and <i>Stella vacuolata</i>, respectively.","authors":"Umakant Bhoopati Deshmukh, Aharon Oren","doi":"10.1099/ijsem.0.006930","DOIUrl":"10.1099/ijsem.0.006930","url":null,"abstract":"<p><p>The prokaryotic generic names <i>Bogoriella</i> Groth <i>et al</i>. 1997 and <i>Stella</i> Vasilyeva 1985 are illegitimate because they are later homonyms of the genus names <i>Bogoriella</i> Zahlbr. 1928 (Ascomycota) and <i>Stella</i> Massee 1889 (Basidiomycota) (Principle 2 and Rule 51b(4) of the International Code of Nomenclature of Prokaryotes). We therefore propose the replacement names <i>Allobogoriella</i> gen. nov., <i>Allobogoriella caseilytica</i> comb. nov., <i>Allobogoriellaceae</i> fam. nov., <i>Allobogoriellales</i> ord. nov., <i>Allostella</i> gen. nov., <i>Allostella humosa</i> comb. nov. and <i>Allostellaceae</i> fam. nov. as replacement names for the illegitimate prokaryotic names <i>Bogoriella</i>, <i>Bogoriella caseilytica</i>, <i>Bogoriellaceae</i>, <i>Bogoriellales</i>, <i>Stella</i>, <i>Stella humosa</i>, <i>Stella vacuolata</i> and <i>Stellaceae</i>, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Alteromonas algae</i> sp. nov. and <i>Alteromonas ulvae</i> sp. nov., isolated from the seaweed <i>Ulva prolifera</i> dominating green tides in the Yellow Sea.","authors":"Xiaojing Lv, Jin Zhao, Peng Jiang","doi":"10.1099/ijsem.0.006925","DOIUrl":"10.1099/ijsem.0.006925","url":null,"abstract":"<p><p>Strains A079^T and A081^T were isolated from the floating seaweed <i>Ulva prolifera</i>, which is the dominant species of the world's largest green tides in the Yellow Sea. Both strains were Gram-stain-negative, aerobic and motile via a single polar flagellum. The major fatty acids of the two strains were C_16:0, C_17:1 ω8c, C_18:1 ω7c and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). Cells of the strain A079^T were oval or short rod-shaped. Cells of the strain A081^T were rod-shaped. A079^T grew at 10-37 °C, pH 6.0-10.0 and 1-12% (w/v) NaCl. A081^T grew at 10-40 °C, pH 6.0-11.0 and 1-12% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA genes and whole-genome sequences revealed that strains A079^T and A081^T belong to the genus <i>Alteromonas</i>. The average nucleotide identity and digital DNA-DNA hybridization values between strains A079^T, A081^T and related <i>Alteromonas</i> species ranged from 68.98 to 88.75% and 20.40 to 36.40%, respectively. Comparative genomic analysis discovered that A079^T possessed a unique RiPP-like (TP-1161) cluster. Strain A089^T exhibited the potential to utilize ulvan, and an acyl-homoserine lactone synthesis gene cluster was predicted in the strain. These findings reveal possible mechanisms for phycosphere adaptation. Phenotypic, chemotaxonomic, phylogenetic and genomic evidence support that strains A079^T and A081^T represent two novel species of the genus <i>Alteromonas</i>, for which the names <i>Alteromonas algae</i> sp. nov. (type strain A079^T=MCCC 1K09912^T=KCTC 18157^T) and <i>Alteromonas ulvae</i> sp. nov. (type strain A081^T=MCCC 1K09913^T=KCTC 18158^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Aureibaculum koreense</i> sp. nov. and <i>Aureibaculum conchae</i> sp. nov., isolated from the marine molluscs.","authors":"Soo-Bin Kim, Kyung Hyun Kim, Jin-Sook Park","doi":"10.1099/ijsem.0.006933","DOIUrl":"https://doi.org/10.1099/ijsem.0.006933","url":null,"abstract":"<p><p>Facultative anaerobic Gram-negative bacteria, designated strains 2210JD6-5^T and 2308TA14-22^T, were isolated from a seashell and a marine conch, respectively, in the Republic of Korea, and subjected to detailed taxonomic analysis. The cells exhibited catalase and oxidase activity, were non-motile and were rod-shaped. Both novel strains were mesophilic, grew optimally at neutral pH and were slightly halophilic. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences demonstrated that strains 2210JD6-5^T and 2308TA14-22^T were affiliated with the genus <i>Aureibaculum</i>. The digital DNA-DNA hybridization and average nucleotide identity values between strains 2210JD6-5^T and 2308TA14-22^T and type strains of the most closely related species were below the cut-off thresholds. Both strains possessed DNA G+C contents of 33.5 mol%. The predominant cellular fatty acids and the sole respiratory quinone in these two strains were iso-C_15 : 0, iso-C_15 : 1 G and iso-C_17 : 0 3-OH and MK-6, respectively. Strains 2210JD6-5^T and 2308TA14-22^T shared polar lipids, such as phosphoethanolamine, aminophospholipid, unidentified aminolipid and unidentified lipid. Based on phylogenetic, genomic relatedness and chemotaxonomic characteristics, strains 2210JD6-5^T and 2308TA14-22^T represent a novel species within the genus <i>Aureibaculum</i>, for which the names <i>Aureibaculum koreense</i> sp. nov. (2210JD6-5^T=KCTC 102167^T=JCM 37322^T) and <i>Aureibaculum conchae</i> sp. nov. (2308TA14-22^T=KCTC 102250^T=JCM 37321^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Umbribacter vaginalis</i> gen. nov., sp. nov.: novel bacterium isolated from the human vagina.","authors":"Sujatha Srinivasan, Susan M Strenk, May A Beamer, Tina L Fiedler, Sean Proll, Gabriela R Acevedo-Oquendo, Gina M Bonura, G A Nagana Gowda, Daniel Raftery, Sharon L Hillier, David N Fredricks","doi":"10.1099/ijsem.0.006931","DOIUrl":"https://doi.org/10.1099/ijsem.0.006931","url":null,"abstract":"<p><p>Gram-variable obligately anaerobic novel bacteria DNF00809 and PR-HUZ-602407-17 were isolated from vaginal fluid samples from women with bacterial vaginosis (BV) in two independent studies conducted in different laboratories. They each displayed ≥99.9% 16S rRNA gene sequence identity to the uncultured bacterial clone sequence AY738656 designated as <i>Eggerthella</i>-like vaginal bacterium (ELVB) and shared 100% 16S rRNA gene sequence identity with each other. Studies using molecular bacterial identification have associated ELVB sequences with BV, higher risk for human immunodeficiency virus acquisition and development of pelvic inflammatory disease in women. Given the clinical significance of this bacterium, we characterized the novel bacterium designated DNF00809^T using biochemical, genotypic and phylogenetic analyses. DNF00809^T was a coccobacillus that was non-motile, non-spore forming, asaccharolytic, proteolytic and indole negative. Fatty acid methyl ester analysis for DNF00809^T indicated C_14 : 0, C_16 : 0, C_16 : 0 dimethyl acetal and C_18 : 1 <i>cis</i>9 to be the major fatty acids. Whole genomic DNA G+C content was 46.1 mol%. Phylogenetic and phylogenomic analyses indicate that DNF00809^T represents a novel genus and novel species within the <i>Eggerthellaceae</i> family. We propose the name <i>Umbribacter vaginalis</i> gen. nov., sp. nov. with DNF00809^T representing the type strain of this species (=DSM 118866^T=CCUG77988^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 10","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Neorhizobium tunisiense</i> sp. nov., reclassification of <i>Neorhizobium xiangyangii</i> as <i>Pseudorhizobium xiangyangii</i> comb. nov. and proposal of <i>Terrirhizobium terrae</i> gen. nov., comb. nov. to accommodate the divergent species <i>Aliirhizobium terrae</i>.","authors":"Imed Sbissi, Dhouha Boussoufa, Ikram Kammoun, Rihab Hamra, Hana Dali, Mohamed Tarhouni, Maher Gtari","doi":"10.1099/ijsem.0.006912","DOIUrl":"10.1099/ijsem.0.006912","url":null,"abstract":"<p><p>The taxonomic affiliations, genomic divergence and ecological potential of strains IRAMC 0178ᵀ and IRAMC 0179, isolated from nodules of <i>Retama raetam</i> growing in coastal northern Tunisia, were investigated using genome-scale phylogenomics, average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) and protein metrics (average amino acid identity, percentage of conserved proteins and core-proteome average amino acid identity). Taxogenomic, ecogenomic and phenotypic analyses were used to classify strains IRAMC 0178ᵀ and IRAMC 0179 - each isolated from nodules of distinct <i>R. raetam</i> trees in northern Tunisia - and to revise the taxonomic status of <i>Neorhizobium xiangyangii</i> and <i>Aliirhizobium terrae</i>. Strain IRAMC 0178ᵀ (=HAMBI 3839=CGMCC 1.65424=DSM 119302^T) was placed in <i>Neorhizobium</i>, showing low genomic similarity to known species (dDDH <45% and ANI <93%) supporting its recognition as a novel genomic species. Its distinct phenotypic characteristics further justify the proposal of the new species <i>Neorhizobium tunisiense</i> sp. nov. Further genomic analyses revealed discrepancies in the current taxonomy, supporting the reclassification of <i>N. xiangyangii</i> as <i>Pseudorhizobium xiangyangii</i> comb. nov. and the transfer of <i>A. terrae</i> to the newly proposed genus <i>Terrirhizobium terrae</i> gen. nov., comb. nov. Several non-nodulating <i>Neorhizobium</i> strains, including IRAMC 0178ᵀ, either lacked or carried incomplete <i>nod</i> and <i>nif</i> gene sets, suggesting a loss of symbiotic capacity across these lineages. However, strains retained diverse plant growth-promoting traits and stress-adaptive functions. Notably, <i>N. tunisiense</i> sp. nov. exhibited genomic features indicative of a rhizosphere-associated lifestyle, such as osmoprotective systems, moderate growth rates and metabolic versatility. These results highlight the diverse evolutionary pathways within <i>Rhizobiaceae</i>, including both symbiotic and non-symbiotic lineages, and emphasize the overlooked genomic diversity with potential implications for sustainable agriculture.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Flavobacterium movens</i> sp. nov. and <i>Flavobacterium mesophilum</i> sp. nov., two novel bacteria isolated from rhizosphere soil of Litchi (<i>Litchi chinensis</i> Sonn.).","authors":"Yijie Dong, Xing Wang, Yang Liu, Guang-Da Feng, Qing Yao, Honghui Zhu","doi":"10.1099/ijsem.0.006908","DOIUrl":"10.1099/ijsem.0.006908","url":null,"abstract":"<p><p>Two aerobic, Gram-stain-negative, gliding motility and rod-shaped bacterial strains, designated as B11^T and D4^T, were isolated from rhizosphere soil of Litchi (<i>Litchi chinensis</i> Sonn.) in Guangzhou, Guangdong Province, P.R. China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains B11^T and D4^T belonged to the genus <i>Flavobacterium</i> and shared the highest similarity to <i>Flavobacterium ginsenosidimutans</i> THG 01^T (98.2%) and <i>Flavobacterium daemonensis</i> JCM 19455^T (98.0%), respectively. The DNA G+C contents of strains B11^T and D4^T were 33.9 and 34.1 mol%, respectively. Both the genome-derived average nucleotide identity and digital DNA-DNA hybridization values between the two strains and their closely related type species <i>Flavobacterium sharifuzzamanii</i> KCTC 62405^T and <i>Flavobacterium denitrificans</i> DSM 15936^T were below 39.3 and 90.5%, respectively. They all took iso-C_15:0 and summed feature 3 (C_16:1 <i>ω</i>6<i>c</i> and/or C_16:1  <i>ω</i>7<i>c</i>) as the major fatty acids. The polar lipids of strain B11^T contained phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL1-2) and four unidentified lipids (L1-4), while strain D4^T took phosphatidylethanolamine (PE), three aminophospholipids (APL1-3) and four unidentified lipids (L1-4). Menaquinone-6 was their predominant quinone. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analyses, strains B11^T and D4^T should be considered as two novel species of the genus <i>Flavobacterium</i>, for which the names <i>Flavobacterium movens</i> (type strain B11^T=GDMCC 1.4103^T=JCM 36423^T) and <i>Flavobacterium mesophilum</i> (type strain D4^T=GDMCC 1.4105^T=JCM 36424^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145053533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three novel <i>Luteimonas</i> species from faeces of <i>Cygnus atratus</i>: <i>Luteimonas changyuni</i> sp. nov., <i>Luteimonas yanweni</i> sp. nov. and <i>Luteimonas qiangzhengi</i> sp. nov.","authors":"Yuqing Liu, Jiajia Ma, Hao Zhou, Qiang Hou, Jinlv Liu, Han Wang, Qun Xiao, Wenbo Luo, Ji Pu, Jing Yang, Lili Ren, Jianguo Xu","doi":"10.1099/ijsem.0.006923","DOIUrl":"https://doi.org/10.1099/ijsem.0.006923","url":null,"abstract":"<p><p>Six Gram-stain-negative, aerobic and rod-shaped bacterial strains, designated as MJ145^T, MJ204, MJ174^T, MJ246, MJ146^T and MJ293, were isolated from faeces of <i>Cygnus atratus</i>, in Qinghai, PR China. The six strains grew at pH 7.0-9.0, 4-37 °C; strains MJ145^T, MJ204, MJ174^T and MJ246 grew at 0-6.0% NaCl; MJ146^T and MJ293 grew at 0-5.0% NaCl. The 16S rRNA gene sequence analysis revealed that strains MJ145^T and MJ204 exhibited the highest similarity (98.21% and 98.03 %, respectively) to <i>Luteimonas terricola</i> BZ92r^T, strains MJ174^T and MJ246 showed the highest similarity (98.36% and 98.16 %, respectively) to '<i>Luteimonas granuli</i>' Gr-4 and strains MJ146^T and MJ293 showed the highest similarity (96.36% and 96.38 %, respectively) to '<i>Luteimonas dalianensis</i>' OB44-3. Combined with the phylogenomic tree, it showed that strain pairs MJ145^T/MJ204 and MJ174^T/MJ246 were most closely related to <i>L. terricola</i> BZ92r^T, while strain pair MJ146^T/MJ293 was most closely related to '<i>L. dalianensis</i>' OB44-3, <i>Luteimonas suaedae</i> LNNU 24178^T, <i>Luteimonas salinilitoris</i> C3-2-a3^T, <i>Luteimonas endophytica</i> RD2P54^T and <i>Luteimonas salinisoli</i> SJ-92^T. The average nt identity and digital DNA-DNA hybridization values between the strains and the other members of the genus <i>Luteimonas</i> were below the established thresholds of 95-96% and 70 %, respectively. The respiratory ubiquinone-8 was detected in the strains MJ145^T, MJ174^T and MJ146^T. The major fatty acids of all three strains (MJ145^T, MJ174^T and MJ146^T) were iso-C_15 : 0 and summed feature 9 (C_16 : 0 10-methyl and/or iso-C_17 : 1 <i> ω</i>9c). All three strains contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. Based on polyphasic characterization, these six strains should be considered to be three novel species within genus <i>Luteimonas</i>, for which the names <i>Luteimonas changyuni</i> sp. nov. (MJ145^T/MJ204, type strain MJ145^T=GDMCC 1.4700^T=JCM 37394^T), <i>Luteimonas yanweni</i> sp. nov. (MJ174^T/MJ246, type strain MJ174^T=GDMCC 1.4862^T=JCM 37396^T) and <i>Luteimonas qiangzhengi</i> sp. nov. (MJ146^T/MJ293, type strain MJ146^T=GDMCC 1.4701^T=JCM 37395^T) are proposed, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Candidatus</i> List No. 7.","authors":"Marko Kostovski, Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006847","DOIUrl":"10.1099/ijsem.0.006847","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statutes of the International Committee on Systematics of Prokaryotes - July 2025 revision.","authors":"David R Arahal, Markus Göker, Richard L Hahnke, Célia M Manaia, Edward R B Moore, Aharon Oren, Iain C Sutcliffe, Stefano Ventura","doi":"10.1099/ijsem.0.006918","DOIUrl":"10.1099/ijsem.0.006918","url":null,"abstract":"<p><p>The members of the International Committee on Systematics of Prokaryotes (ICSP) have recently voted on a proposed revision of its statutes. We here present the outcome of the vote and the text of the revised statutes, as approved by the ICSP.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Mesohalobacter salilacus</i> sp. nov., a novel halotolerant, flexirubin-type pigment-producing bacterium of the family <i>Flavobacteriaceae</i>.","authors":"Shuai-Bo Han, Yang-Huan Yu, Xiao-Long He, Jin-Jia Liang, Min Wu, Can Chen, Xue-Wei Xu","doi":"10.1099/ijsem.0.006905","DOIUrl":"https://doi.org/10.1099/ijsem.0.006905","url":null,"abstract":"<p><p>A Gram-staining-negative, non-motile, aerobic, rod-shaped bacterium, designated 14752^T, was isolated from a saline lake in Xinjiang Uygur Autonomous Region, China. The strain was subjected to a taxonomic study using a polyphasic approach. Strain 14752^T was able to grow at 4-40 ℃ (optimum 28 ℃), pH 6.0-7.5 (optimum 7.0) and with 1.0-9.0% NaCl (optimum 3-4%, w/v). Phylogenetic and sequence similarity analysis of 16S rRNA gene sequences indicated that strain 14752^T was grouped with <i>Mesohalobacter halotolerans</i> WDS2C27^T, with the highest similarity of 98.13%. In comparison, this similarity between strain 14752^T and other valid published species was less than 92%. The average nucleotide identity based on blast alignments, digital DNA-DNA hybridization and average amino identity values between strain 14752^T and <i>M. halotolerans</i> WDS2C27^T were 80.13%, 22.60% and 83.91%, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was menaquinone 6 (MK-6), the major fatty acids included anteiso-C_15:0, iso-C_15:0 and iso-C_15:1 G. The major polar lipids included phosphatidylethanolamine, two uncharacterized aminolipids, one uncharacterized amino phospholipid, one uncharacterized phospholipid and one uncharacterized lipid. The genomic DNA G+C content of the strain 14752^T was 34.1 mol%. According to the physiological, genotypic, genomic and chemotaxonomic features revealed in this investigation, it is recommended that strain 14752^T represents a novel species of the genus <i>Mesohalobacter</i>, for which the name <i>Mesohalobacter salilacus</i> sp. nov. is proposed. The type strain is 14752^T (=KCTC 72044^T=MCCC 1K03765^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}