Brachymonas wangyanguii sp. nov., isolated from the coal samples in Shanxi Province of China.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-07-01 DOI:10.1099/ijsem.0.006837

Fangyu Gao, Mei Zhang, Qiang Yang, Lijun Zhao, Junyao Xie, Caixin Yang, Xiaojuan Du, Zhiming Kang, Ji Pu, Han Zheng, Jianguo Xu, Kui Dong

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Abstract

Two Gram-negative, oxidase-positive, catalase-positive, non-spore-forming, motile, short-rod strains (G13^T and G34) were isolated from in situ coal block samples. Phylogenetic analyses based on 16S rRNA genes and 718 core genes indicated that both strains belonged to the genus Brachymonas, with the closest relative being Brachymonas chironomi AIMA4^T as their closest relative. Average nucleotide identity and digital DNA-DNA hybridization values were 79.4-89.27% and 23.6-37.0% below the species-level thresholds. Further chemotaxonomic analysis revealed that both strains possessed C_16:1 ω7c and/or C_16:1 ω6c, C_18:1 ω7c and C_16:0 as major cellular fatty acids, with Q-8 as the predominant respiratory quinone. The polar lipid profile was dominated by diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phenotypic and phylogenetic evidence support the proposal that strain G13^T represents a novel species of the genus Brachymonas, with the name Brachymonas wangyanguii sp. nov. The type strain is G13^T (=GDMCC 1.4691^T=JCM 37746^T).

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山西省煤炭样品中分离的王延贵短单胞菌。

从原位煤块样品中分离到革兰氏阴性、氧化酶阳性、过氧化氢酶阳性、不产孢子、可运动的短棒状菌株G13T和G34。基于16S rRNA基因和718个核心基因的系统发育分析表明，两株菌株均属于短爪单胞菌属，最近的亲缘关系为chironomi Brachymonas AIMA4T。平均核苷酸同一性和数字DNA-DNA杂交值分别低于种水平阈值79.4 ~ 89.27%和23.6 ~ 37.0%。进一步的化学分类分析表明，这两种菌株的细胞脂肪酸主要为C16:1 ω7c和/或C16:1 ω6c、C18:1 ω7c和C16:0，呼吸醌以Q-8为主。极性脂质谱以二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺为主。表型和系统发育证据支持菌株G13T代表Brachymonas属的新种，命名为Brachymonas wangyanguii sp. nov.，型菌株为G13T （=GDMCC 1.4691T=JCM 37746T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.