International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Brevibacterium koreense</i> sp. nov., a moderately halophilic bacterium isolated from jogae-jeotgal, a Korean fermented seafood.","authors":"Sohee Nam, Yujin Kim, Min Ji Lee, Yeon Bee Kim, Jeong Ui Yun, Mi-Ja Jung, Hye Seon Song, Se Hee Lee, Seok-Jun Kim, Tae Woong Whon","doi":"10.1099/ijsem.0.006722","DOIUrl":"10.1099/ijsem.0.006722","url":null,"abstract":"<p><p>A novel Gram-stain-positive, non-spore-forming, non-motile and rod-shaped bacterium, designated strain CBA3109^T, was isolated from jogae-jeotgal (fermented clam), a traditional Korean fermented seafood. Strain CBA3109^T showed growth at 10-30 °C (optimum, 25 °C) and pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-15% (w/v) NaCl (optimum, 5%). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CBA3109^T belonged to the genus <i>Brevibacterium</i>, with the highest similarities to <i>Brevibacterium aurantiacum</i> NCDO 739^T (98.26%) and <i>B. antiquum</i> VKM Ac-2118^T (98.14%). Strain CBA3109^T contained MK-8(H₂) as the major menaquinone. The cell wall peptidoglycan contained <i>meso</i>-diaminopimelic acid. The major fatty acids (>10%) were anteiso-C_{15 :  0} and anteiso-C_{17 :  0}. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values between strain CBA3109^T and the closest species were 86.2-87.0% and 31.1-33.0 %, respectively. The DNA G+C content of strain CBA3109^T was 62.7%. Based on the morphological, phylogenetic, chemotaxonomic and genotypic data, strain CBA3109^T represents a novel species of the genus <i>Brevibacterium</i>, for which the name <i>Brevibacterium koreense</i> sp. nov. is proposed. The type strain is CBA3109^T (= KACC 23387^T = DSM 117564^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Christiangramia sediminicola</i> sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment.","authors":"Jia-Xuan Wang, Jing Wang, Ning-Hua Liu, Qi-Long Qin, Ping-Yi Li, Xiao-Yan Song, Xiu-Lan Chen, Yu-Zhong Zhang, Xi-Ying Zhang","doi":"10.1099/ijsem.0.006703","DOIUrl":"10.1099/ijsem.0.006703","url":null,"abstract":"<p><p>A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212^T, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5-35 °C and with 0.5-5.5% NaCl (w/v). It was able to reduce nitrate to nitrite and hydrolyse starch, gelatin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy genes showed that strain SM2212^T belonged to the genus <i>Christiangramia</i> within the family <i>Flavobacteriaceae</i>, sharing the highest 16S rRNA gene sequence similarity with the type strain of <i>Christiangramia echinicola</i> (97.1%) and 96.2-97.0% 16S rRNA gene sequence similarity with those of other known species in the genus. The major cellular fatty acids were summed feature 3 (C_16:1 <i>ω7с</i> and/or C_16:1 <i>ω6с</i>), iso-C_15:0, iso-C_17: 03-OH, summed feature 9 (iso-C_17:1 <i> ω9c</i> and/or 10-methyl C_16:0) and C_16:0. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content of strain SM2212^T was 37.0 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM2212^T and type strains of closely related known <i>Christiangramia</i> species were below 22.2 and 79.7%, respectively. Based on the polyphasic taxonomic analysis in this study, strain SM2212^T is considered to represent a novel species in the genus <i>Christiangramia</i>, for which the name <i>Christiangramia sediminicola</i> is proposed. The type strain is SM2212^T (=KCTC 92980^T=MCCC 1K07684^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphasic taxonomic description of <i>Streptomyces okerensis</i> sp. nov. and <i>Streptomyces stoeckheimensis</i> sp. nov. and their biotechnological potential.","authors":"Imen Nouioui, Eveline Derr, Alina Zimmermann, Marlen Jando, Gabriele Pötter, Sarah Kirstein, Meina Neumann-Schaal, Cathrin Spröer, Boyke Bunk, Yvonne Mast","doi":"10.1099/ijsem.0.006716","DOIUrl":"10.1099/ijsem.0.006716","url":null,"abstract":"<p><p><i>Streptomyces</i> strains DSM 116494^T and DSM 116496^T were isolated from sediment samples of the River Oker in Braunschweig, Germany, and subjected to a polyphasic taxonomic study and genome mining for specialized secondary metabolites. Phenotypic, genetic and genomic data confirmed the assignment of these strains to the <i>Streptomyces</i> genus. Pairwise 16S rRNA gene sequence similarity values between the strains and validly named <i>Streptomyces</i> species reached 99.5 and 99.7% for strains DSM 116494^T and DSM 116496^T, respectively. Genome-based phylogeny demonstrated that <i>Streptomyces pilosus</i> and <i>Streptomyces griseoflavus</i> species were the close relatives to strain DSM 116494^T, while <i>Streptomyces vinaceus</i> species was the nearest neighbour to strain DSM 116496^T. Digital DNA-DNA hybridization and average nucleotide identity comparisons of the genomic sequence of the strains and their close phylogenomic relatives revealed that values were below the determined threshold of 70 and 95-96% for prokaryotic species demarcation, respectively. The strains were distinguished from their close neighbours based on biochemical, chemotaxonomic and enzymatic data. Given these results, the strains merit being affiliated to novel species within the genus <i>Streptomyces</i>, for which the names <i>Streptomyces okerensis</i> sp. nov. (=OG2.3^T=DSM 116494^T=KCTC 59408^T) and <i>Streptomyces stoeckheimensis</i> sp. nov. (=OG3.14^T=DSM 116496^T=KCTC 59410^T) are proposed. Strains DSM 116494^T and DSM 116496^T harboured several biosynthetic gene clusters encoding potentially novel antimicrobial and anticancer compounds. Crude extracts of strains DSM 116494^T and DSM 116496^T inhibited the growth of Gram-negative bacteria (<i>Escherichia coli</i> ΔtolC, <i>Proteus vulgaris</i>) and a multi-drug-resistant Gram-positive, <i>Staphylococcus aureus</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptomyces solicamelliae</i> sp. nov. and <i>Streptomyces solicathayae</i> sp. nov., isolated from the rhizosphere soil of <i>Camellia oleifera</i> and <i>Cathaya argyrophylla</i>.","authors":"Yaxi Zheng, Ting Tang, Ping Mo, Zhilong Wu, Kaiqin Li, Wansheng Zou, Yun Wang, Baiyuan Li","doi":"10.1099/ijsem.0.006714","DOIUrl":"10.1099/ijsem.0.006714","url":null,"abstract":"<p><p>Two novel actinobacteria strains, HUAS MG47^T and HUAS YS2^T, were isolated from the rhizosphere soil of <i>Camellia oleifera</i> and <i>Cathaya argyrophylla</i> collected from Changde City, Hunan Province, China. These two strains could form rectiflexible spore chains consisting of short cylindrical spores with smooth surfaces, suggesting that they had typical characteristics of the genus <i>Streptomyces</i>. The cell wall peptidoglycan contained ll-diaminopimelic acid as a diagnostic amino acid. The predominant menaquinones were MK-9 (H₆), MK-9 (H₈) and MK-9 (H₄). The genome sizes of HUAS MG47^T and HUAS YS2^T were 7.35 and 7.88 Mbp with a G+C content of 72.3 and 72.0 mol%, respectively. Phylogenetic analysis based on the 16S rRNA gene and five housekeeping gene sequences (<i>atpD</i>, <i>gyrB</i>, <i>recA</i>, <i>rpoB</i> and <i>trpB</i>) indicated that strains HUAS MG47^T and HUAS YS2^T formed an evolutionary lineage with <i>Streptomyces tritici</i> CGMCC 4.7393^T. However, the values of multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization between these two strains and their closely related relatives indicated that they were clearly different from other known <i>Streptomyces</i> species. Consequently, based on the polyphasic taxonomic study, HUAS MG47^T and HUAS YS2^T represent two novel <i>Streptomyces</i> species for which the names <i>Streptomyces solicamelliae</i> sp. nov. (HUAS MG47^T=MCCC 1K09365^T=JCM 37293^T) and <i>Streptomyces solicathayae</i> sp. nov. (HUAS YS2^T=MCCC 1K08940^T=JCM 36737^T) are proposed, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Rhodococcus parequi</i> sp. nov., a new species isolated from equine farm soil closely related to the pathogen <i>Rhodococcus equi</i>.","authors":"José A Vazquez-Boland, Jorge Val-Calvo, Fabien Duquesne, Francesca Decorosi, Carlo Viti, Sandrine Petry, Mariela Scortti","doi":"10.1099/ijsem.0.006679","DOIUrl":"10.1099/ijsem.0.006679","url":null,"abstract":"<p><p>We present the description of the new species, <i>Rhodococcus parequi</i>, found during phylogenomic investigations of a global collection of strains identified as <i>Rhodococcus</i> (<i>Prescottella</i>) <i>equi</i>. Strain PAM 2766^T was isolated from horse-breeding farm soil in Normandy, France, and was indistinguishable from <i>R. equi</i> based on the usual identification tests. Whole-genome phylogenetic analyses located PAM 2766^T in the same <i>Rhodococcus</i> sublineage as <i>R. equi</i>, together with <i>Rhodococcus agglutinans</i>, <i>Rhodococcus defluvii</i>, <i>Rhodococcus soli</i>, <i>Rhodococcus subtropicus</i>, <i>Rhodococcus spongiicola</i> and <i>Rhodococcus xishaensis</i>. PAM 2766^T is most closely related to, but sufficiently distinct from, <i>R. equi</i> DSM 20307 ^T to be considered a separate species. The average nt identity (ANI) and average aa identity (AAI) values are 88.60% and 92.35, respectively, well below the species cutoff. The PAM 2766^T draft genome is ~5.3 Mb in size with 68.98% G+C mol content. PAM 2766^T is strictly aerobic and non-motile and produces smooth, creamy to buff-coloured colonies very similar to those of <i>R. equi</i>. It phenotypically differs from the latter by the ability to grow at 5 °C, a strongly positive urease test at 24 h and specificities in the carbon and nitrogen source utilization profile as determined by phenotype microarray screens. Our data indicate that PAM 2766^T belongs to a novel species, for which the name <i>Rhodococcus parequi</i> sp. nov. is proposed. <i>R. parequi</i> was avirulent in macrophage infection assays and is assumed to be non-pathogenic. The type strain is PAM 2766^T (=CETC 30995^T=NCTC 14987^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 74, part 11 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006619","DOIUrl":"10.1099/ijsem.0.006619","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fructilactobacillus frigidiflavus</i> sp. nov., a pigmented species, and <i>Levilactobacillus lettrarii</i> sp. nov., a propionate-producing species isolated from sourdough.","authors":"Vi D Pham, Michael G Gänzle","doi":"10.1099/ijsem.0.006726","DOIUrl":"10.1099/ijsem.0.006726","url":null,"abstract":"<p><p>The sourdough isolates FUA3702, FUA3912 and FUA3913^T, as well as FUA3695^T and FUA3914, could not be identified to known species of the <i>Lactobacillaceae</i>. The 16S rRNA gene sequences of FUA3702 and FUA3913, FUA3695 and FUA3914 were>99% identical to <i>Fructilactobacillus sanfranciscensis</i> and <i>Levilactobacillus lanxiensis</i>, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FUA3913^T when compared to <i>Fl. sanfranciscensis</i> were 83.67 and 26.60%, respectively. In addition, strains FUA3702, FUA3912 and FUA3913^T produce different levels of a yellow C30 carotenoid, but pigmentation has not been described in <i>Fl. sanfranciscensis</i>. The ANI and dDDH values of FUA3695^T and FUA3914 when compared to <i>Lv. langxiensis</i> were 95.22 and 61.20%, respectively. In addition, FUA3695 and FUA3914 convert lactate to 1,2-propanediol and further to propionate. The conversion of lactate to propionate by a single strain has not been documented for any of the species in the <i>Lactobacillaceae</i>. Based on the genomic and physiological characteristics, we proposed the novel species <i>Fructilactobacillus frigidiflavus</i> sp. nov. FUA3913^T (=DSM 118650^T=LMG 33758^T) and <i>Levilactobacillus lettrarii</i> sp. nov. FUA3695^T (=DSM 118651^T=LMG 33759^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ascoidea xinghuacunensis</i> sp. nov., a novel ascomycetous yeast species from Xinghuacun Fenjiu old workshop, Shanxi province of China.","authors":"Pan Zhen, Hai-Yan Zhu, Ying Han, Lu-Jun Luo, Liang-Chen Guo, Shuang Hu, Yu-Hua Wei, Zhang Wen, Da-Yong Han, Zhong-Wei Lin, Feng-Yan Bai, Pei-Jie Han","doi":"10.1099/ijsem.0.006700","DOIUrl":"10.1099/ijsem.0.006700","url":null,"abstract":"<p><p>Four yeast strains belonging to the ascomycetous yeast genus <i>Ascoidea</i> were isolated from the mixture of remnants of steamed sorghum, Daqu powder and the fermented grain that fell off during transportation on the ground in the workshop which were collected in Xinghuacun Fenjiu old workshop, Shanxi province, PR China. We propose the name <i>Ascoidea xinghuacunensis</i> sp. nov. (holotype: strain CGMCC 2.7787) for the new species, which differs phenotypically from the other three species in this genus: <i>Ascoidea asiatica</i>, <i>Ascoidea rubescens</i> and <i>Ascoidea tarda</i> by its ability to grow at 37 °C. This study represents the first isolation of <i>Ascoidea</i> species within the borders of China and the initial report of the <i>Ascoidea</i> species detailing the isolation from the Baijiu brewing environment.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Limibacterium fermenti</i> gen. nov., sp. nov., a novel member of the family <i>Dysgonomonadaceae</i> isolated from the Chinese strong aroma-type Baijiu fermentation system.","authors":"Yang Gu, Huilin Wang, Xia Zhang, Zhiyun Peng, Jian Su, Dong Zhao, Jia Zheng","doi":"10.1099/ijsem.0.006702","DOIUrl":"10.1099/ijsem.0.006702","url":null,"abstract":"<p><p>An anaerobic isolate, designated me31^T, was isolated from pit mud in Yibin, Sichuan Province, PR China. Phylogenetic results based on 16S rRNA gene sequence showed that strain me31^T belongs to the family <i>Dysgonomonadaceae</i>, and the most closely related isolated relatives were <i>Seramator thermalis</i> SYSU GA16112^T (93.65%) and <i>Proteiniphilum propionicum</i> JNU-WLY501^T (93.22%). The DNA G+C content was 44.26 mol%. The ANI and AAI values between strain me31^T and the closely related strains were 69.25-71.18% and 69.59-71.15%, respectively. Cells of strain me31^T were Gram-stain-negative, rod-shaped and non-motile. Growth of strain me31^T was observed at 25-37 °C, pH 6.0-8.0 and a salt tolerance range of 0-1.0% (w/v). The predominant respiratory quinone was MK-9. The major fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and C_{17 : 0} 2OH. The polar lipids of strain me31^T were found to consist of phosphatidylethanolamine, three unidentified phospholipids, three unidentified phosphoglycolipids, one unidentified phosphoglycolipid, one unidentified lipid, two unidentified glycolipids and one aminophosphoglycolipid. According to the results of morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic analysis, strain me31^T represents a novel species of a novel genus of the family <i>Dysgonomonadaceae</i>, for which the name <i>Limibacterium fermenti</i> gen. nov., sp. nov. is proposed. The type strain is me31^T (=GDMCC 1.4237^T=KCTC 25756^T=WMCC 10035^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Niallia tiangongensis</i> sp. nov., isolated from the China Space Station.","authors":"Junxia Yuan, Wende Zhang, Lei Dang, Yan Song, Zhao Yin, Ziwei He, Kanyan Xu, Pei Guo, Hong Yin","doi":"10.1099/ijsem.0.006693","DOIUrl":"10.1099/ijsem.0.006693","url":null,"abstract":"<p><p>Understanding the characteristics of microbes during long-term space missions is essential for safeguarding the health of astronauts and maintaining the functionality of spacecraft. In this study, a Gram-positive, aerobic, spore-forming, rod-shaped strain JL1B1071^T was isolated from the surface of hardware on the China Space Station. This strain belongs to the genus <i>Niallia</i>, with its closest relative being <i>Niallia circulans</i> ATCC 4513^T. The genome of JL1B1071^T is 5 166 230 bp in size, with a G+C content of 35.6 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between JL1B1071^T and <i>N. circulans</i> ATCC 4513^T are 83.3 and 27.5%, respectively, both below the recommended thresholds for species delineation. The major cellular fatty acids were anteiso-C_15:0 and iso-C_15:0. The major quinone was menaquinone-7 (MK-7). Notably, strain JL1B1071^T demonstrates a unique ability to hydrolyse gelatin, suggesting that it can utilize gelatin as a substrate in nutrient-limited environments. Genomic analysis of JL1B1071^T revealed two conserved signature indels in the GAF domain-containing protein and DNA ligase D protein, which are specific to the genus <i>Niallia</i>. Additionally, structural and functional differences in proteins BshB1 and SplA were identified, which may enhance biofilm formation, oxidative stress response and radiation damage repair, thereby aiding its survival in the space environment. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome annotation, strain JL1B1071^T was considered a novel species within the genus <i>Niallia</i> and is proposed to be named <i>Niallia tiangongensis</i> sp. nov. The type strain is JL1B1071^T (=GDMCC 1.4642=KCTC 43715).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}