Pseudidiomarina salilacus sp. nov., a novel bacterium of the genus Pseudidiomarina isolated from sediment in Yuncheng Salt Lake.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-07-01 DOI:10.1099/ijsem.0.006846

Hui-Ying Yu, Jia-Tan Wei, Chuan-Xu Wang, Zhuo Wang, Ya-Jie Niu, Wen-Jun Li, Bao-Zhu Fang, Hao-Ran Guo, Xin Li

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Abstract

Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91^T, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.0 (optimum pH 7.0-8.0) and with 1-11.0% NaCl (w/v, optimum 3%). Strain YC-516-91^T was positive for hydrolysis of Tween 80 and starch, but negative for nitrate reduction, urease, H₂S production, hydrolysis of Tween 40 or gelatin. Phylogenetic analysis based on 16S rRNA gene and genome sequences showed that YC-516-91^T, YC-516-48 and YC-516-93 belonged to the genus Pseudidiomarina. 16S rRNA gene sequence similarity values of YC-516-91^T to the closest related type strains Pseudidiomarina atlantica MCCC T A10513^T, Pseudidiomarina terrestris 1APP75-27a^T and Pseudidiomarina donghaiensis 908033^T were below 97.99%. Strains YC-516-91^T, YC-516-48 and YC-516-93 have average nucleotide identity (ANI) values of 99-100%, digital DNA-DNA hybridization values of 92.1-97.4% and average amino acid (AAI) values of 99-100%, suggesting that they belong to the same species. These strains are closely related to P. atlantica MCCC 1A10513^T, with ANI and AAI of around 92-93%, below the species delineation cut-off, thus indicating that they represent a novel species. The genome of strain YC-516-91^T was 2,723,173 bp, and the genomic DNA G+C content was 50.24 mol%. The only respiratory quinone was Q-8, and the major fatty acids (>10%) were iso-C_17 : 0 and iso-C_15 : 0. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, glycolipid, unidentified aminolipids and two unidentified polar lipids. Thus, strains YC-516-91^T, YC-516-48 and YC-516-93 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. Strain YC-516-91^T is proposed to represent a novel species named Pseudidiomarina salilacus sp. nov., within the genus Pseudidiomarina. The type strain is YC-516-91^T (=MCCC 1K09529^T=KCTC 8778^T).

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从运城盐湖沉积物中分离到的一种新细菌——salilacus sp. nov.。

从运城盐湖沉积物中分离到3株革兰氏阴性好氧棒状黄色素细菌，编号为YC-516-91T、YC-516-48和YC-516-93。生长条件为15 ~ 42℃（32 ~ 37℃），pH 6.0 ~ 10.0 (7.0 ~ 8.0)， NaCl浓度1 ~ 11.0% （w/v，最适3%）。菌株YC-516-91T对Tween 80和淀粉的水解呈阳性，但对硝酸盐还原、脲酶、H2S产生、Tween 40和明胶的水解呈阴性。基于16S rRNA基因和基因组序列的系统发育分析表明，YC-516-91T、YC-516-48和YC-516-93均属于伪didiomarina属，其中YC-516-91T与最近亲缘型菌株大西洋伪didiomarina MCCC T A10513T、陆地伪didiomarina terrestris 1APP75-27aT和东海伪didiomarina 908033T的16S rRNA基因序列相似性值均低于97.99%。菌株YC-516-91T、YC-516-48和YC-516-93的平均核苷酸同源性（ANI）值为99 ~ 100%，数字DNA-DNA杂交值为92.1 ~ 97.4%，平均氨基酸（AAI）值为99 ~ 100%，表明它们属于同一种。这些菌株与P. atlantica MCCC 1A10513T亲缘关系密切，ANI和AAI均在92-93%左右，低于种划分界限，表明它们是一个新种。菌株YC-516-91T基因组全长2,723,173 bp，基因组DNA G+C含量为50.24 mol%。唯一的呼吸醌为Q-8，主要脂肪酸为iso-C17: 0和iso-C15: 0。主要极性脂类包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂、糖脂、未识别的氨基脂和两种未识别的极性脂类。因此，菌株YC-516-91T、YC-516-48和YC-516-93在化学分类、表型和系统发育特征上代表了一个新种。菌株YC-516-91T是伪didiomarina salilacus sp. nov.中的一个新种。型应变为YC-516-91T （=MCCC 1K09529T=KCTC 8778T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.