International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Cupriavidus phytorum</i> sp. nov., isolated from <i>Zea mays</i> L. rhizosphere in Mexico and <i>Mimosa diplotricha</i> Sauvalle root nodule in Taiwan.","authors":"Belén Chávez-Ramírez, Leslie-Mariana Morales-Ruiz, Violeta Larios-Serrato, Gerardo Chavez-Esquivel, Daniel Cortes-Avalos, J Antonio Ibarra, Paulina Estrada de Los Santos","doi":"10.1099/ijsem.0.006709","DOIUrl":"10.1099/ijsem.0.006709","url":null,"abstract":"<p><p>Several <i>Cupriavidus</i> and <i>Burkholderia</i> strains from the north of Mexico were isolated during the description of <i>Cupriavidus</i> strains. <i>Cupriavidus alkaliphilus</i>, <i>Cupriavidus plantarum</i> and <i>Cupriavidus agave</i> were later described as novel species. Next, the Genomic Encyclopedia of Bacteria and Archaea project retrieved the genome sequences of several strains from the above species. Standard comparative genomic analysis showed that <i>C. alkaliphilus</i> MLR2-44 was taxonomically misclassified. This strain, isolated from the <i>Zea mays</i> rhizosphere, was analysed by average nucleotide identity (ANI), revealing that <i>Cupriavidus nantongensis</i> was the closest species (95.6%). However, the digital DNA-DNA hybridization (dDDH) resulted in values lower than 68% for any type strain of <i>Cupriavidus</i> species. Moreover, it was also found that <i>Cupriavidus taiwanensis</i> LMG 19430, isolated from <i>Mimosa diplotricha</i> root nodules, was closer to strain MLR2-44^T (99.9% ANI and 99.8% dDDH), instead of being associated with the type strain of <i>C. taiwanensis</i> LMG 19424^T (94.4% ANI and 54.6% dDDH), thus making strains MLR2-44^T and LMG 19430 a single genomic species. Accordingly, the polyphasic analysis showed that these strains represent a new species; therefore, we propose that strains MLR2-44^T (=CDBB B-2066^T=TSD-312^T) and LMG 19430 encompass a novel <i>Cupriavidus</i> species with the name of <i>Cupriavidus phytorum</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Mycobacterium servetii</i> sp. nov., a novel rapid-growing nontuberculous mycobacterial species recovered from a human patient in Zaragoza, Spain.","authors":"Alexander Tristancho-Baró, Sergio Buenestado-Serrano, Darío García de Viedma, Ana Milagro, Natalia Burillo, Sara Sanz, Ana Isabel López-Calleja, Daniel Peñas-Utrilla, Marta Guardingo, María Jesús García, Jesús Viñuelas-Bayón","doi":"10.1099/ijsem.0.006727","DOIUrl":"10.1099/ijsem.0.006727","url":null,"abstract":"<p><p>In this study, we describe a novel rapid-growing <i>Mycobacterium</i> species isolated from a clinical specimen obtained from the lower respiratory tract of a patient with ciliary dysfunction, bronchiectasis and exacerbated respiratory symptoms. A comprehensive phenotypic characterization was conducted, including the establishment of a MALDI-TOF MS profile. Additionally, whole-genome sequencing was performed to assess overall genomic relatedness indices and conduct phylogenetic comparative analyses. These findings allowed us to characterize a previously unrecognized rapid-growing <i>Mycobacterium</i> species, for which we propose the name <i>Mycobacterium servetii</i> (=DSM 118141; =CECT 31091).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pelomonas baiyunensis</i> sp. nov., <i>Pelomonas candidula</i> sp. nov., <i>Pelomonas dachongensis</i> sp. nov., <i>Pelomonas lactea</i> sp. nov., <i>Pelomonas margarita</i> sp. nov., <i>Pelomonas nitida</i> sp. nov., <i>Pelomonas parva</i> sp. nov. and <i>Roseateles rivi</i> sp. nov., isolated from streams in China.","authors":"Huibin Lu, Guannan Li, Shan Yu","doi":"10.1099/ijsem.0.006707","DOIUrl":"10.1099/ijsem.0.006707","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Eight Gram-stain-negative, aerobic and motile strains (BYS78W&lt;sup&gt;T&lt;/sup&gt;, BYS87W&lt;sup&gt;T&lt;/sup&gt;, BYS96W&lt;sup&gt;T&lt;/sup&gt;, BYS180W&lt;sup&gt;T&lt;/sup&gt;, DC23W&lt;sup&gt;T&lt;/sup&gt;, DXS20W&lt;sup&gt;T&lt;/sup&gt;, LKC17W&lt;sup&gt;T&lt;/sup&gt; and LYH14W&lt;sup&gt;T&lt;/sup&gt;) were isolated from streams in China and showed the highest 16S rRNA gene sequence similarities with the species of genus &lt;i&gt;Pelomonas&lt;/i&gt;. The calculated average nucleotide identity, digital DNA-DNA hybridization and average amino acid sequence identity values among these eight strains and other closely related strains were less than 89.8, 39.1 and 89.4 %, respectively, indicating that each of the eight strains should represent an independent novel species. The further reconstructed phylogenomic tree showed that seven isolated strains clustered closely with the &lt;i&gt;Pelomonas&lt;/i&gt; strains. Only strain BYS180W&lt;sup&gt;T&lt;/sup&gt; clustered with the &lt;i&gt;Roseateles&lt;/i&gt; strains. The phylogenomic tree also showed that the taxonomic status of the recently published species (&lt;i&gt;Paucibacter sediminis&lt;/i&gt;, &lt;i&gt;Roseateles aquae&lt;/i&gt;, '&lt;i&gt;Roseateles caseinilyticus&lt;/i&gt;', '&lt;i&gt;Roseateles cellulosilyticus&lt;/i&gt;', &lt;i&gt;Roseateles subflavus&lt;/i&gt; and &lt;i&gt;Roseateles violae&lt;/i&gt;) was questionable. '&lt;i&gt;R. caseinilyticus&lt;/i&gt;' and '&lt;i&gt;R. cellulosilyticus&lt;/i&gt;' should be reassigned to the genus &lt;i&gt;Pelomonas&lt;/i&gt; as &lt;i&gt;Pelomonas caseinilytica&lt;/i&gt; P7&lt;sup&gt;T&lt;/sup&gt; (=KACC 22504&lt;sup&gt;T&lt;/sup&gt;=TBRC 15694&lt;sup&gt;T&lt;/sup&gt;) and &lt;i&gt;Pelomonas cellulosilytica&lt;/i&gt; P8&lt;sup&gt;T&lt;/sup&gt; (=KACC 22505&lt;sup&gt;T&lt;/sup&gt;=TBRC 15695&lt;sup&gt;T&lt;/sup&gt;), respectively. &lt;i&gt;P. sediminis&lt;/i&gt;, &lt;i&gt;R. aquae&lt;/i&gt;, &lt;i&gt;R. subflavus&lt;/i&gt; and &lt;i&gt;R. violae&lt;/i&gt; should be reassigned to the genus &lt;i&gt;Kinneretia&lt;/i&gt; as &lt;i&gt;Kinneretia sediminis&lt;/i&gt;, &lt;i&gt;Kinneretia aquae&lt;/i&gt;, &lt;i&gt;Kinneretia subflava&lt;/i&gt; and &lt;i&gt;Kinneretia violae&lt;/i&gt;, respectively. Combining the above descriptions, strains BYS78W&lt;sup&gt;T&lt;/sup&gt;, BYS87W&lt;sup&gt;T&lt;/sup&gt;, BYS96W&lt;sup&gt;T&lt;/sup&gt;, DC23W&lt;sup&gt;T&lt;/sup&gt;, DXS20W&lt;sup&gt;T&lt;/sup&gt;, LKC17W&lt;sup&gt;T&lt;/sup&gt; and LYH14W&lt;sup&gt;T&lt;/sup&gt; should represent seven novel species of the genus &lt;i&gt;Pelomonas&lt;/i&gt;, for which the names &lt;i&gt;Pelomonas baiyunensis&lt;/i&gt; sp. nov. (type strain BYS87W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4090&lt;sup&gt;T&lt;/sup&gt;=KCTC 8151&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Pelomonas candidula&lt;/i&gt; sp. nov. (type strain BYS78W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4089&lt;sup&gt;T&lt;/sup&gt;=KCTC 8152&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Pelomonas dachongensis&lt;/i&gt; sp. nov. (type strain DC23W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4087&lt;sup&gt;T&lt;/sup&gt;=KCTC 8148&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Pelomonas lactea&lt;/i&gt; sp. nov. (type strain DXS20W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4088&lt;sup&gt;T&lt;/sup&gt;=KCTC 8147&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Pelomonas margarita&lt;/i&gt; sp. nov. (type strain LKC17W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4086&lt;sup&gt;T&lt;/sup&gt;=KCTC 8146&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Pelomonas nitida&lt;/i&gt; sp. nov. (type strain BYS96W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4091&lt;sup&gt;T&lt;/sup&gt;=KCTC 8150&lt;sup&gt;T&lt;/sup&gt;) and &lt;i&gt;Pelomonas parva&lt;/i&gt; sp. nov. (type strain LYH14W&lt;sup&gt;T&lt;/sup&gt;=GDMCC 1.4276&lt;sup&gt;T&lt;/sup&gt;=KCTC 8301&lt;sup&gt;T&lt;/sup&gt;) are proposed, respectively. Strain BYS180W&lt;sup&gt;T&lt;/sup&gt; should represent a novel species of the genus &lt;i&gt;Roseateles&lt;/i&gt;, for which the ","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Vanderwaltozyma urihicola</i> sp. nov., a yeast species isolated from rotting wood and beetles in a Brazilian Amazonian rainforest biome.","authors":"Flávia B M Alvarenga, Katharina O Barros, Thiago M Batista, Gisele F L Souza, Ana Raquel O Santos, Maxwell A Abegg, Trey K Sato, Chris Todd Hittinger, Marc-André Lachance, Carlos A Rosa","doi":"10.1099/ijsem.0.006718","DOIUrl":"10.1099/ijsem.0.006718","url":null,"abstract":"<p><p>Five yeast isolates belonging to a candidate for novel species were obtained from rotting wood and the gut of a passalid beetle larva in a site of Amazonian rainforest biome in Brazil. Sequence analysis of the Internal Transcribed Spacer (ITS)-5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of the genus <i>Vanderwaltozyma</i>. The closest relative of the novel species is <i>Vanderwaltozyma huisunica</i>. These species differs due to 44 nt substitutions and 21 indels in the sequences of the ITS region, as well as by 15 substitutions and four indels in the sequences of the D1/D2 domains. A phylogenomic analysis of the <i>Vanderwaltozyma</i> species with genomes sequenced showed that this novel species is an outgroup to the other species of this genus. We propose the name <i>Vanderwaltozyma urihicola</i> sp. nov. (CBS 18107^T, MycoBank MB 856975) to accommodate these isolates. The species is homothallic, producing one to two ascospores per ascus. The habitat of <i>V. urihicola</i> is rotting wood in the Brazilian Amazonian rainforest biome.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Rhodotorula tropicalis</i> sp. nov., a novel red yeast of the order Sporidiobolales isolated from Thailand, Indonesia and Japan.","authors":"Pannida Khunnamwong, Panadda Nualthaisong, Kanokwan Kingphadung, Masako Takashima, Takashi Sugita, I Nyoman Sumerta, Atit Kanti, Hiroko Kawasaki, Savitree Limtong","doi":"10.1099/ijsem.0.006701","DOIUrl":"10.1099/ijsem.0.006701","url":null,"abstract":"<p><p>Six yeast strains, representing a novel anamorphic species of the genus <i>Rhodotorula</i>, were investigated in this study. Among them, three strains, SU21, SU16 and SU14, were obtained from three different fruiting bodies of wild mushrooms in Thailand. One strain (ISM36-1) was isolated from soil in Japan, and two strains were isolated from soil (14Y315) and leaf litter (Y15Kr055) collected in Indonesia. Analysis of the sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the six strains were identical or differed by only one nt substitution in both the D1/D2 domain of the LSU rRNA gene and the ITS regions. <i>Rhodotorula paludigena</i> CBS 6566^T was the most closely related species but with 23-24 nt substitutions in the D1/D2 domain of the LSU rRNA gene and 38-39 nt substitutions in the ITS regions. Phylogenetic analysis based on the concatenated sequences of the ITS regions and D1/D2 domain showed that these strains represent a single species of the <i>Rhodotorula</i> clade that is distinct from other recognized species of the genus. Based on the phylogenetic analysis and phenotypic characteristics, these six strains were assigned to a novel species of the genus <i>Rhodotorula</i>, although sexual reproduction was not observed. The name, <i>Rhodotorula tropicalis</i> sp. nov., is proposed to accommodate the six strains. The holotype is TBRC 14874^T and the ex-type culture is PYCC 8913 (=SU21). The MycoBank number of the novel species is MB 856795.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Curvibacter soli</i> sp. nov., <i>Extensimonas soli</i> sp. nov., <i>Pseudarthrobacter naphthalenicus</i> sp. nov. and <i>Terripilifer ovatus</i> gen. nov., sp. nov., four new species isolated from polluted soil.","authors":"Ze-Shen Liu, Ke-Huan Wang, Xiao-Kang Wang, Man Cai, Mei-Ling Yang, Wen-Ke Yang, De-Feng Li, Shuang-Jiang Liu","doi":"10.1099/ijsem.0.006698","DOIUrl":"10.1099/ijsem.0.006698","url":null,"abstract":"<p><p>A taxonomic study was conducted on four bacterial strains isolated from the soil of a coking plant. Phylogenetic analysis showed that the four strains belonged to three families: <i>Comamonadaceae</i>, <i>Micrococcaceae</i> and <i>Roseiarcaceae</i>. Identification of the 16S rRNA gene exhibited that their sequence similarities were between 94.96 and 98.98% when compared to known and validly nominated species. Their genomes ranged from 3.4 to 7.2 Mb, with DNA G+C molar contents varying from 62.3 to 67.2%. The average nucleotide identities ranged from 71.4 to 92.3%, and digital DNA-DNA hybridization values were 19.7-47.0% when comparing them with closely related type species, supporting the classification of these four strains. Functional analysis demonstrated that strain H3Y2-7^T was robustly resistant to chromate (VI) and arsenite (III) and was able to grow on aromatic compounds including naphthalene as carbon sources even in the presence of chromate (VI). These features reflect its ability to treat combined pollutants and adapt to a polluted environment. Based on the analysis of polyphasic taxonomy, we propose the four bacterial strains representing novel species, namely <i>Curvibacter soli</i> sp. nov. (type strain H39-3-26^T=JCM 36178^T=CGMCC 1.61344^T), <i>Extensimonas soli</i> sp. nov. (type strain H3M7-6^T=JCM 36176^T=CGMCC 1.61336^T), <i>Pseudarthrobacter naphthalenicus</i> sp. nov. (type strain H3Y2-7^T=JCM 36482^T=CGMCC 1.61323^T) and <i>Terripilifer ovatus</i> gen. nov., sp. nov. (type strain H3SJ34-1^T=JCM 36465^T=CGMCC 1.61333^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic revision of the family <i>Aurantimonadaceae</i>: proposal of <i>Dennerimonas</i> gen. nov., <i>Mesocryomonas</i> gen. nov., <i>Rathsackimonas</i> gen. nov. and <i>Plantimonas</i> gen. nov., along with the reclassification of <i>Jeongeupella</i> Jiang <i>et al</i>. 2024 as a later heterotypic synonym of <i>Antarcticirhabdus</i> Du <i>et al</i>. 2023.","authors":"Imed Sbissi, Hana Dali, Rihab Hamra, Emeni Boujemaa, Mohamed Aziz Haj Nasr, Faten Ghodhbane-Gtari, Maher Gtari","doi":"10.1099/ijsem.0.006733","DOIUrl":"10.1099/ijsem.0.006733","url":null,"abstract":"<p><p>Phylogenetic analysis of the genus <i>Aureimonas</i>, utilizing both 16S rRNA gene sequences and comprehensive whole-genome data, revealed its polyphyletic nature, necessitating a revision to accommodate phylogenetically distinct species. Based on established threshold values for genus demarcation - specifically, 16S rRNA gene similarity, Average Amino Acid Identity and Percentage of Conserved Proteins - a notably substantial divergence was observed within the genus <i>Aureimonas</i>, and the division of <i>Aureimonas</i> into four distinct genera is strongly supported. To address this, we propose the establishment of four new genera: <i>Dennerimonas</i> gen. nov., <i>Mesocryomonas</i> gen. nov., <i>Rathsackimonas</i> gen. nov. and <i>Plantimonas</i> gen. nov. These classifications accommodate species that are significantly divergent from the type species of <i>Aureimonas</i>, thereby more accurately reflecting their distinct evolutionary lineages. Additionally, <i>Aureimonas glaciistagni</i> is proposed to be reclassified within the genus <i>Jiella</i> as <i>Jiella glaciistagni</i> comb. nov. based on phylogenetic evidence indicating a closer evolutionary relationship to <i>Jiella</i> species than to other members of <i>Aureimonas</i>. Our analysis, which included assessments of 16S rRNA gene similarity, Average Nucleotide Identity, and digital DNA-DNA hybridization values exceeding species delineation thresholds, further supports the reclassification of <i>Jeongeupella</i> Jiang <i>et al</i>. 2024 as a later heterotypic synonym of <i>Antarcticirhabdus</i> Du <i>et al</i>. 2023.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fulvimarina uroteuthidis</i> sp. nov., isolated from a marine invertebrate and <i>Uroteuthis</i> (<i>Photololigo</i>) <i>edulis</i>.","authors":"Yea-Lin Moon, Kyung Hyun Kim, Jin-Sook Park","doi":"10.1099/ijsem.0.006683","DOIUrl":"10.1099/ijsem.0.006683","url":null,"abstract":"<p><p>Gram-stain-negative, strictly aerobic bacterium, designated strain 2208YS6-2-32^T, was isolated from a marine invertebrate, <i>Uroteuthis</i> (<i>Photololigo</i>) <i>edulis</i> collected by trawl in the south coastal sea of the Republic of Korea. The cells were motile, short-rod, non-spore-forming and contained carotenoid pigments. Catalase and oxidase activities were positive. According to phylogenetic analysis based on 16S rRNA gene and whole-genome sequence determined that strain 2208YS6-2-32^T belongs to the novel <i>Fulvimarina</i> species and shares the highest 16S rRNA gene similarity with <i>Fulvimarina endophytica</i> 85^T (97.5%). The average nucleotide identity and digital DNA-DNA hybridization values between strain 2208YS6-2-32^T and <i>Fulvimarina endophytica</i> 85^T were 78.3% and 21.9%, respectively. The DNA G+C content was 64.5%. Optimal growth of strain 2208YS6-2-32^T was observed at 28 °C and pH 8 and in the presence of 1-3% (w/v) NaCl. Strain 2208YS6-2-32^T contained ubiquinone-10 as the respiratory quinone, C_18 : 1 ω<i>7c</i> (79.0%) and summed feature 3 (C_{16  :  1} <i>ω</i>7<i>c</i> and/or C_16 : 1 ω6c; 13.5%) as the major fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified aminolipid and seven unidentified lipids. Based on the results of the polyphasic approach, strain 2208YS6-2-32^T represents a novel species of the genus <i>Fulvimarina</i>, for which the name <i>Fulvimarina uroteuthidis</i> sp. nov. is proposed. The type strain is 2208YS6-2-32^T (=KACC 23586^T=JCM 36703^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Methanococcoides cohabitans</i> sp. nov., a marine methylotrophic methanogen isolated from an anaerobic methane-oxidizing enrichment culture.","authors":"Sihan Li, Lewen Liang, Sidra Erum Ishaq, Tariq Ahmad, Yijing Dong, Jialin Hou, Gunter Wegener, Fengping Wang","doi":"10.1099/ijsem.0.006685","DOIUrl":"10.1099/ijsem.0.006685","url":null,"abstract":"<p><p>Enrichment cultures of archaea and bacteria performing the anaerobic oxidation of methane (AOM) regularly contain persistent methanogens. Here, we isolated the marine methanogen <i>Methanococcoides cohabitans</i> sp. nov. strain LMO-2^T from a long-term AOM enrichment culture from the Northern Gulf of Mexico. Strain LMO-2^T is Gram-stain-negative, irregular 0.5-1 µm coccus without flagella. It utilizes a variety of methylated compounds including methanol, monomethylamine, dimethylamine and trimethylamine for growth and methanogenesis. However, it does not grow on formate, acetate, dimethyl sulphate, H₂/CO₂, betaine and choline. The optimal conditions for growth were observed within a temperature range of 30-35 °C, a pH range of 7.0-8.0 and a salinity range of 2-4% NaCl. Based on the similarity and phylogeny of the 16S rRNA gene and genomic sequence, strain LMO-2^T is classified within the genus <i>Methanococcoides</i>. Among the isolated type strains of the genus, strain LMO-2^T exhibited the highest 16S rRNA gene sequence identity with <i>Methanococcoides vulcani</i> SLH33^T (99.4%). The digital DNA-DNA hybridization and average nucleotide identity based on genome sequence showed that strain LMO-2^T shared the highest similarity with <i>Methanococcoides orientis</i> LMO-1^T, with values of 27.3% and 83.4%, respectively. In conclusion, we isolated a methylotrophic methanogen from an AOM culture, and the isolated strain LMO-2^T represented a novel species of the genus <i>Methanococcoides</i>, for which the name <i>Methanococcoides cohabitans</i> sp. nov. is proposed. The type strain is LMO-2^T (=CGMCC 1.18051^T=KCTC 25774^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paenibacillus campi</i> sp. nov., a novel endophytic bacterium isolated from <i>Pennisetum</i> species.","authors":"Ling Yao, Manik Prabhu Narsing Rao, Cheng-Jie Xie, Peng Gao, Shu-Qun Zhang, Guo-Hong Liu, Shun-Gui Zhou","doi":"10.1099/ijsem.0.006708","DOIUrl":"10.1099/ijsem.0.006708","url":null,"abstract":"<p><p>Two facultatively aerobic, Gram-stain-positive, rod-shaped, endospore-forming, endophytic bacteria, designated SGZ-1009^T and SGZ-1014, were isolated from the plant <i>Pennisetum</i> sp. Strain SGZ-1009^T grew at 5-50 °C, pH 4.5-11.0 and tolerated up to 4.5% NaCl (w/v), while strain SGZ-1014 exhibited growth at a temperature range of 10-50 °C, pH range 4.5-11.0 and tolerated up to 4.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genus <i>Paenibacillus</i>, closely related to the reference type strain of <i>Paenibacillus wenxiniae</i> DSM 100576^T (similarity of 97.0%). The genomic relatedness values for both strains, SGZ-1009^T and SGZ-1014, compared to their closest reference strain <i>P. wenxiniae</i> DSM 100576^T, were 81.6% for average nucleotide identity and 26.0% for digital DNA-DNA hybridization, suggesting strain SGZ-1009^T represents a novel species. The genomic DNA G+C content of strain SGZ-1009^T was 46.4%. Both strains shared anteiso-C_15:0 (55.9%) as the predominant fatty acid, menaquinone-7 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Based on these results, strains SGZ-1009^T and SGZ-1014 represent a novel species of <i>Paenibacillus</i>, for which the name <i>Paenibacillus campi</i> sp. nov. is proposed. The type strain is SGZ-1009^T (=MCCC 1K08892^T=GDMCC 1.4280^T=KCTC 43677^T=JCM 36670^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}