International journal of systematic and evolutionary microbiology最新文献

{"title":"Proposal of <i>Actinoplanes capillaceus</i> Matsumoto <i>et al</i>. 2000 as a later heterotypic synonym of <i>Actinoplanes campanulatus</i> (Couch 1963) Stackebrandt and Kroppenstedt 1987 and an emended description of <i>Actinoplanes campanulatus</i>.","authors":"Ping Mo, Yiru Ming, Yuhuan Cao, Kaiqin Li, Wansheng Zou, Yun Wang, Baiyuan Li","doi":"10.1099/ijsem.0.006744","DOIUrl":"https://doi.org/10.1099/ijsem.0.006744","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Xanthomonas bundabergensis</i> sp. nov., <i>Xanthomonas medicagonis</i> sp. nov. and <i>Xanthomonas tesorieronis</i> sp. nov.: three members of group 1 <i>Xanthomonas</i>.","authors":"Daniel J E McKnight, Johanna Wong-Bajracharya, Efenaide B Okoh, Fridtjof Snijders, Fiona Lidbetter, John Webster, Mathew Haughton, Steven P Djordjevic, Daniel R Bogema, Toni A Chapman","doi":"10.1099/ijsem.0.006686","DOIUrl":"10.1099/ijsem.0.006686","url":null,"abstract":"<p><p>Between 1976 and 2010, four bacterial isolates were collected in New South Wales and Queensland, Australia, and stored as part of routine biosecurity surveillance. Recently, these historic isolates were analysed as part of a larger project to enhance the taxonomic accuracy of our culture collection and improve Australia's biosecurity preparedness. Three isolates were collected from <i>Fragaria × ananassa</i>, initially identified as <i>Xanthomonas</i> sp., and one from <i>Medicago sativa</i>, identified as <i>Xanthomonas axonopodis</i> subsp. <i>alfalfae</i>. In this study, we employed modern phenotypic and genomic techniques to further characterize these isolates. Matrix-assisted laser desorption ionization-time of flight MS biotyping and Biolog GEN III MicroPlates confirmed that they are members of the <i>Xanthomonas</i> genus but did not allow for species-level classification. Genome-relatedness indices and phylogenetic analysis confirmed that they were <i>Xanthomonas</i> and revealed that they represent three novel species. The maximum average nucleotide identity and digital DNA-DNA hybridization values observed when comparing the four isolates to all <i>Xanthomonas</i> type strains and each other were 93.9% and 50.7%, respectively. Pathogenesis assays confirmed that two of the isolates are not pathogenic to <i>Fragaria</i>, the plant from which they were isolated. Based on these findings, we propose the names <i>Xanthomonas bundabergensis</i> sp. nov. (DAR 80977^T=ICMP 24943), <i>Xanthomonas medicagonis</i> sp. nov. (DAR 35659^T=ICMP 24942) and <i>Xanthomonas tesorieronis</i> sp. nov. (DAR 34887^T=ICMP 24940).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ureaplasma ceti</i> sp. nov. isolated from the gastric fluid of a spotted dolphin (<i>Stenella attenuata</i>).","authors":"Takao Segawa, Yu Nakajima, Keiko Yamamoto, Shoko Kishima, Takuya Itou","doi":"10.1099/ijsem.0.006712","DOIUrl":"10.1099/ijsem.0.006712","url":null,"abstract":"<p><p><i>Ureaplasma</i> sp. (OM1^T, OM4 and OM7) novel strains were isolated from the gastric fluid of a spotted dolphin (<i>Stenella attenuata</i>). These strains were phenotypically and genotypically characterized, and compared with known species of the genus <i>Ureaplasma</i>. All isolated strains hydrolysed urea and metabolized arginine, but did not produce acid from glucose. All strains were propagated using pleuropneumonia-like organisms medium supplemented with serum and urea under aerobic and anaerobic atmospheric conditions at 37 °C. Transmission electron microscopy revealed a typical mollicute cellular morphology. Analysis of the 16S rRNA gene sequence indicated that the most closely related, validly named type strain was <i>Ureaplasma gallorale</i> ATCC 43346^T (89.4% similarity). The average nucleotide identity and digital DNA-DNA hybridization values among strain OM1^T and closely related species were lower than the accepted thresholds for describing novel prokaryotic species at the genomic level. Based on the genomic, phenotypic and phylogenetic properties, the strains represent a novel species of the genus <i>Ureaplasma</i>, for which the name <i>Ureaplasma ceti</i> sp. nov. with type strain OM1^T (=DSM 116106^T=JCM 39153^T) is proposed. The genomic G+C content and draft genome sizes of the type strain were 31.7% and 889 711 bp, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Marivivens marinum</i> sp. nov., isolated from tidal flat, Gochang, South Korea.","authors":"Min-Seok Kang, Hyung-Seop Kim, Jae-Yon Yu, Sathiyaraj Srinivasan, Sang-Seob Lee","doi":"10.1099/ijsem.0.006735","DOIUrl":"10.1099/ijsem.0.006735","url":null,"abstract":"<p><p>A Gram-negative, rod-shaped, non-motile, aerobic bacterium, designated MK-3^T, was isolated from shallow seawater in Gochang, Republic of Korea. Growth of strain MK-3^T occurred at 15-40 °C (30 °C), pH 6.0-7.0 (pH 7.0) and in the presence of 2-3 % NaCl (2%). Phylogenetic analysis based on the 16S rRNA gene sequence placed strain MK-3^T within the family <i>Roseobacteraceae</i>. It exhibited sequence similarities of 95.7% with <i>Salipiger marinus</i> CK-I3-6^T, <i>Salipiger aestuarii</i> DSM 22011^T and <i>Salipiger pentaromativorans</i> P9^T; 95.4% with <i>Ruegeria alba</i> 1NDH52C^T, <i>Salipiger manganoxidans</i> VSW210^T and <i>Salipiger thiooxidans</i> DSM 10146^T; 95.3% with <i>Marivivens donghaensis</i> AM-4^T and <i>Histidinibacterium lentulum</i> B17^T; and 95.2% with <i>Marivivens geojensis</i> FJ12^T. Phylogenetic and phylogenomic analyses consistently demonstrated that strain MK-3^T formed a distinct lineage within the genus <i>Marivivens</i>, clustering with its closest relatives. The major fatty acids were C_18 : 1 <i> ω7</i>c<i>/</i>C1_8 : 1 <i> ω6</i>c, C_18 : 1 <i> ω7</i>c 11-methyl and C_16 : 0. The genome length of strain MK-3^T was 3.3 Mbp, and the DNA G+C content was 62.8 mol%. The strain contained Q-10 as the major ubiquinone. The polar lipids consisted of three phosphatidylinositol mannosides and a diphosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain MK-3^T represents a novel species in the genus <i>Marivivens</i>, for which the name <i>Marivivens marinum</i> sp. nov. is proposed. The type strain is MK-3^T (=KEMB 21417^T=KCTC 8294^T=JCM 36630^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A whole-genome study suggests a taxonomic rearrangement of <i>Saccharopolyspora subtropica</i> as a later heterotypic synonym of <i>Saccharopolyspora thermophila</i> and <i>Saccharopolyspora spinosporotrichia</i> as a later heterotypic synonym of <i>Saccharopolyspora erythraea</i>.","authors":"Guendouz Dif, Nadjette Djemouai, Atika Meklat, Abdelghani Zitouni","doi":"10.1099/ijsem.0.006731","DOIUrl":"10.1099/ijsem.0.006731","url":null,"abstract":"<p><p>The genus <i>Saccharopolyspora</i> consists of Gram-positive bacteria that are found in nature. The <i>Saccharopolyspora</i> genus, belonging to the family <i>Pseudonocardiaceae</i> and order <i>Pseudonocardiales</i>, has shown considerable phylogenetic and taxonomic overlaps. Therefore, a phylogenetic, phylogenomic and comparative genomic investigation was conducted to elucidate the taxonomic position of four <i>Saccharopolyspora</i> taxa. Analysis of the 16S rRNA genes showed that the sequence similarity between the tested species ranged from 95.59 to 100%, with <i>Saccharopolyspora hirsuta</i> VKM Ac-666^T as the type species. Moreover, the analysis of digital DNA-DNA hybridization revealed that <i>Saccharopolyspora subtropica</i> CGMCC 4.7206ᵀ and <i>Saccharopolyspora thermophila</i> JCM 10664ᵀ were 97.97% and <i>Saccharopolyspora erythraea</i> DSM 40517ᵀ and <i>Saccharopolyspora spinosporotrichia</i> JCM 10303ᵀ reached 99.9%. In addition, the average nucleotide identity values for all the combinations exceed 99%, indicating little genomic variation for these strains. Our genomic data strongly indicate the close genetic affinity between <i>S. subtropica</i> Wu <i>et al.</i> 2016 and <i>S. thermophila</i> Liu <i>et al.</i> 2001 and <i>S. spinosporotrichia</i> Zhou <i>et al.</i> 1998 and <i>S. erythraea</i> corrig. (Waksman 1923) Labeda 1987, which support their reclassification.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Psychrobacter saeujeotis</i> sp. nov., a novel halophilic bacterium isolated from salted shrimp jeotgal.","authors":"Eiseul Kim, Yinhua Cai, Seung-Min Yang, Woojung Lee, Hae-Yeong Kim","doi":"10.1099/ijsem.0.006734","DOIUrl":"10.1099/ijsem.0.006734","url":null,"abstract":"<p><p>A novel bacterial species, designated FBL11^T, was isolated from salted shrimp jeotgal, a traditional Korean fermented food sampled in the Republic of Korea. This strain showed the highest 16S rRNA gene sequence similarity to <i>Psychrobacter halodurans</i> strain F2608^T (NR_181578.1) at 98.3%. The genome size of strain FBL11^T was 3,294,493 bp with a G+C content of 42.5 mol%. Computation of relatedness indicates that strain FBL11^T shares the highest relatedness of 78.5% with <i>Psychrobacter fulvigenes</i> strain KC-40^T (NZ_CAJGZP01) and 25.2% with <i>Psychrobacter piechaudii</i> strain CIP110854^T (NZ_FUGE01), with values clearly below the cut-offs for species distinction. Polyphasic characterization using biochemical tests and matrix-assisted laser desorption/ionization time-of-flight MS analysis confirmed these findings. Strain FBL11^T grew at 10-30 °C (optimum, 20-30 °C) with 0-15% NaCl (w/v; optimum, 3-6%). Analysis of biosynthetic gene clusters responsible for secondary metabolite production revealed that strain FBL11^T generates unique products such as beta-lactone and redox-cofactors within this genus. Based on the genomic and phenotypic data obtained, we propose that strain FBL11^T represents a novel species, for which we propose the name <i>Psychrobacter saeujeotis</i> sp. nov. (type strain FBL11^T=KACC 23745^T=KCTC 8655^T=JCM 37231^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: <i>Sulfitobacter sediminis</i> gen. nov.,sp. nov., a novel halophilic bacterium isolated from tidal flat sediment.","authors":"Jaincy N Jayan, Ke Dong, Sathiyaraj Srinivasan, Sang-Seob Lee","doi":"10.1099/ijsem.0.006740","DOIUrl":"10.1099/ijsem.0.006740","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Dentiradicibacter hellwigii</i> gen. nov., sp. nov., isolated from a secondary infected root canal in the human oral cavity.","authors":"Sibylle Bartsch, Annette Wittmer, Ann-Kathrin Weber, Meina Neumann-Schaal, Jacqueline Wolf, Sabine Gronow, Jake David Turnbull, Christian Tennert, Georg Häcker, Fabian Cieplik, Ali Al-Ahmad","doi":"10.1099/ijsem.0.006690","DOIUrl":"10.1099/ijsem.0.006690","url":null,"abstract":"<p><p>A motile, rod-shaped and anaerobic strain WK13^T was isolated from a secondary root canal infection of a human tooth. WK13^T cells were Gram-stain-negative, catalase-positive and oxidase-negative. The major fatty acids (≥ 5.0%) were C_16 : 0, C_18 : 0, C_16 : 1 <i> ω</i>7c, C_18 : 1 <i> ω</i>9c and C_18 : 2 <i> ω</i>6,9c. The DNA G+C content was 57.94 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylserine, diphosphatidylglycerol, phosphatidylcholine and lysophosphatidylcholine. There were no respiratory quinones detectable. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WK13^T belongs to the class <i>Betaproteobacteria</i>. WK13^T showed a 93.6% and 93.5% 16S rRNA gene sequence similarity to the most closely related cultured species, <i>Propionivibrio pelophilus</i> strain DSM 12018^T and <i>Propionivibrio dicarboxylicus</i> strain DSM 5885^T, respectively. On the basis of physiological and biochemical data, the isolate is considered to represent a novel species of a new genus in the class <i>Betaproteobacteri</i>a, for which we propose the name <i>Dentiradicibacter hellwigii</i> gen. nov., sp. nov. The type strain is WK13^T (=DSM 112713^T=NCTC 14938^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11881992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Microbacterium candidum</i> sp. nov. and <i>Microbacterium capsulatum</i> sp. nov., isolated from waste landfill.","authors":"Wen Wen, Yuanyuan Zhao, Bing Xie, Jie Lv","doi":"10.1099/ijsem.0.006715","DOIUrl":"10.1099/ijsem.0.006715","url":null,"abstract":"<p><p>Two Gram-stain-positive, oxidase-negative, non-motile and rod-shaped strains (ASV49^T and ASV81^T) were isolated from a waste landfill in Shanghai, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains are associated with members of the genus <i>Microbacterium</i>. Strains ASV49^T and ASV81^T were most closely related to <i>Microbacterium rhizosphaerae</i> JCM 31396^T and <i>Microbacterium bovistercoris</i> CCTCC AA 2018025^T with 98.5 and 98.0% 16S rRNA gene sequence similarities, respectively. The genomic DNA G+C contents of strains ASV49^T and ASV81^T were 69.5 and 70.5 mol %, respectively. Strains ASV49^T and ASV81^T exhibited the highest average nucleotide identity values of 81.7 and 85.3% against their closest relatives <i>Microbacterium xylanilyticum</i> JCM 13591^T and <i>M. rhizosphaerae</i> JCM 31396^T, respectively. Corresponding digital DNA-DNA hybridization values were 25.1 and 30.9%, respectively. Strains ASV49^T and ASV81^T grew optimally at pH 7.0 and 28 °C on Reasoner's 2A. Strain ASV81^T produced capsules, but ASV49^T did not. Both isolates shared the following chemotaxonomic characteristics in common: the major fatty acids of anteiso-C_15 : 0, iso-C_16 : 0 and anteiso-C_17 : 0; the respiratory quinones of MK-11, MK-12, MK-13 and MK-10; the polar lipids of diphosphatidylglycerol and phosphatidylglycerol; and the cell wall amino acids of ornithine, alanine, glycine and glutamic acid. Based on the genomic, phenotypic and chemical characteristics, strains ASV49^T and ASV81^T represent two novel species of the genus <i>Microbacterium</i>, for which the names <i>Microbacterium candidum</i> sp. nov. and <i>Microbacterium capsulatum</i> sp. nov. are proposed. The type strains are ASV49^T (=GDMCC 1.4109^T=JCM 36480^T) and ASV81^T (=GDMCC 1.4110^T=JCM 36481^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>Bifidobacterium faecale</i> Choi <i>et al.</i> 2014 as a later heterotypic synonym of <i>Bifidobacterium adolescentis</i> Reuter 1963.","authors":"Shinnosuke Okuhama, Hidenori Takahashi, Yasunori Nakayama, Akihito Endo","doi":"10.1099/ijsem.0.006706","DOIUrl":"10.1099/ijsem.0.006706","url":null,"abstract":"<p><p><i>Bifidobacterium faecale</i> CU 3-7^T (=JCM 19861^T=KACC 17904^T) was identified as a novel species primarily through phylogenetic analyses of the 16S rRNA gene, heat shock protein 60 (<i>hsp60</i>) and <i>dnaJ1</i> sequences, and <i>in vitro</i> DNA-DNA hybridization (DDH) compared to <i>Bifidobacterium adolescentis</i>. However, genome-based taxonomic approaches have not been studied yet. This study reassessed the taxonomic relationship between <i>B. faecale</i> and <i>B. adolescentis</i> using overall genome-associated indices. The type strains of the two species possessed heterogeneous copies of the 16S rRNA gene within their genomes, with similarities below the species threshold of 98.7-99.0%. Therefore, we were unable to assess the 16S rRNA gene similarity-based taxonomic relationships. This indicated that the 16S rRNA gene is not an appropriate marker for studying the taxonomy of <i>B. adolescentis</i>-associated taxa. The type strains exhibited an ortho average nucleotide identity uncorrected value of 97.7% and a digital DDH value of 80.7%, indicating that they should be assigned to a single taxon. A genome-based re-assessment indicated that <i>B. faecale</i> should be reclassified as a later heterotypic synonym of <i>B. adolescentis</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}