International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Antrihabitans spumae</i> sp. nov., a novel bacterium isolated from stable foams formed in wastewater treatment plants and emended description of the genus <i>Antrihabitans</i>.","authors":"Jhon A Suescún-Sepúlveda, Raúl Riesco, Steve Petrovski, Jacques Soddell, Vartul Sangal, Amanda L Jones, Fernando Sánchez-Juanes, Robert J Seviour, Michael Goodfellow, Martha E Trujillo","doi":"10.1099/ijsem.0.006695","DOIUrl":"https://doi.org/10.1099/ijsem.0.006695","url":null,"abstract":"<p><p>Three strains, designated as J27, J71^T and J72, belonging to the genus <i>Antrihabitans</i>, were isolated from stable foams formed in activated sludge wastewater treatment plants (WWTPs) in New South Wales, Australia. Phenotypic and genomic analyses revealed that these strains belong to the <i>Nocardiaceae</i> family and are closely related to <i>Antrihabitans stalagmiti</i>. However, distinct genomic and physiological characteristics, including overall genomic relatedness indices, phylogenomic analysis, genomic metabolic profiles and MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry), confirmed their status as a new species.Ecologically, these strains showed a wide metabolic versatility, like enhanced membrane transport systems for amino acids, metals and phosphate, as well as the ability to synthesize mycolic acids, contributing to their hydrophobic nature and involvement in foam stabilization. Their adaptations likely provide a competitive advantage in WWTPs, where they persist in nutrient-rich, metal-laden and foam-stabilizing environments. The species did not present the typical rod-coccus cycle, described previously as a defining characteristic of the <i>Antrihabitans</i> genus. Based on their unique genomic, phenotypic and ecological features, we propose the name <i>Antrihabitans spumae</i> sp. nov., with strain J71^T (JCM 34493^T, NCIMB 15450^T) designated as the type strain. Additional strains include J27 (JCM 33914, NCIMB 15449) and J72 (NCIMB 15448).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptococcus wuxiensis</i> sp. nov., <i>Streptococcus jiangnanensis</i> sp. nov. and <i>Streptococcus fermentans</i> sp. nov.: three novel species of genus <i>Streptococcus</i> isolated from human breast milk.","authors":"Ye Zhou, Jianxin Zhao, Paul Ross, Catherine Stanton, Wei Chen, Bo Yang","doi":"10.1099/ijsem.0.006674","DOIUrl":"https://doi.org/10.1099/ijsem.0.006674","url":null,"abstract":"<p><p>Three novel coccoid-shaped strains, designated 21WXBC0057M1^T, 21WXBC0044M1^T and BJSWXB5TM5^T, were isolated from human breast milk in Wuxi, Jiangsu Province, China. These strains were facultative anaerobes, catalase-negative and Gram-positive. Through a comprehensive analysis of rRNA genes, protein-coding housekeeping genes and genomic phylogeny, we identified these strains as belonging to the genus <i>Streptococcus</i>. Specifically, strain 21WXBC0057M1^T was phylogenetically most closely related to <i>Streptococcus infantis,</i> strain 21WXBC0044M1^T was most closely related to <i>Streptococcus oralis</i> and strain BJSWXB5TM5^T displayed similarities to <i>Streptococcus australis, Streptococcus peroris</i> and <i>S. infantis</i>. The pairwise average nucleotide identity and digital DNA-DNA hybridization values for these three strains were below 95 and 70%, respectively, indicating that they occupied evolutionary branches distinct from all previously validly published <i>Streptococcus</i> species. Distinctive phenotypic characteristics discriminated these novel species from the type strains of their most closely related species. The major cellular fatty acids in the three strains were C_16 : 0 and C_18 : 0. Genome annotation and a thorough examination of carbohydrate-active enzyme distribution highlighted the observation that all strains possessed extensive capabilities for carbohydrate metabolism, particularly human milk oligosaccharides utilization. Thus, based on these findings, we proposed the classification of the strains as representing three novel species within the genus <i>Streptococcus: Streptococcus wuxiensis</i> sp. nov. (type strain 21WXBC0057M1^T=GDMCC 1.4126^T=KCTC 25760^T), <i>Streptococcus jiangnanensis</i> sp. nov. (type strain 21WXBC0044M1^T= GDMCC 1.4127^T=KCTC 25762^T) and <i>Streptococcus fermentans</i> sp. nov. (type strain BJSWXB5TM5^T=GDMCC 1.4130^T=KCTC 25759^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Methanobacterium aridiramus</i> sp. nov., a methanogen isolated from potential methane hydrate bearing area offshore southwestern Taiwan.","authors":"Kuan-Yi Lee, Mei-Chin Lai, Saulwood Lin, Shu-Jung Lai, Wei-Ling Zhang, Wen-Chieh Chen, Yi-Ting You, Sue-Yao Wu, Chuan-Chuan Hung, Jiun-Yan Ding, Yang-Zhi Zhou, Chao-Jen Shih, Yen-Chi Wu, Jingjing Zhao, Yin Li, Wangchuan Xiao, Chih-Hung Wu, Hangying Zhang, Guowen Dong, Wanling Qiu, Song Wang, Sheng-Chung Chen","doi":"10.1099/ijsem.0.006658","DOIUrl":"10.1099/ijsem.0.006658","url":null,"abstract":"<p><p>A novel mesophilic, hydrogenotrophic methanogen, strain CWC-01^T, was isolated from the sediment sample of the piston core collected at station 3 at Good Weather Ridge area offshore southwestern Taiwan from ORI-902A cruise in 2009. Cells of strain CWC-01^T are rod-shaped, measuring 0.4-0.5 µm in width and 1.0-2.0 µm in length. Strain CWC-01^T utilizes formate and H₂/CO₂ to produce methane, but not secondary alcohol, methanol, ethanol, methylamines or acetate as catabolic substrates. The optimal growth conditions are 37 °C, 0.043 M NaCl and pH 6.3. Growth effectors tests indicate tryptone, but not acetate and yeast extract, could stimulate the growth of strain CWC-01. Phylogenetic analysis of 16S rRNA gene reveals that strain CWC-01^T is most closely related to <i>Methanobacterium petrolearium</i> Mic5c12^T, with 96.63% identity. Genome size of strain CWC-01^T is 1.98 Mb and it is the smallest genome size of genus. The genomic DNA G+C content obtained from the genome sequence of strain CWC-01^T is 44.15 mol%. Based on these phenotypic, phylogenetic and genomic analyses, we propose that strain CWC-01^T represents a novel species in genus <i>Methanobacterium</i>, for which the name <i>Methanobacterium aridiramus</i> sp. nov. is proposed. The type strain is CWC-01^T (=BCRC AR10053^T=NBRC 113991^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudoalteromonas ardens</i> sp. nov., <i>Pseudoalteromonas obscura</i> sp. nov. and <i>Pseudoalteromonas umbrosa</i> sp. nov. isolated from the coral <i>Montipora capitata</i> on a reef in Kāne'ohe Bay, O'ahu, Hawai'i.","authors":"Emily M Thomas, Emma M Smith, Erin Papke, Maximillian D Shlafstein, Silvia Grant-Beurmann, Lívia C Coelho, Alison C Trott, Claudia C Häse, David K Oline, Patrick Videau, Jimmy H Saw, Wendy K Strangman, Blake Ushijima","doi":"10.1099/ijsem.0.006681","DOIUrl":"https://doi.org/10.1099/ijsem.0.006681","url":null,"abstract":"<p><p>Strains P94^T, B95^T and R96^T were isolated from apparently healthy fragments of the coral <i>Montipora capitata</i>, which were resistant to <i>Vibrio coralliilyticus</i> infection, from the reef surrounding Moku o Lo'e in Kāne'ohe Bay, O'ahu, Hawai'i, USA, and were taxonomically evaluated using a polyphasic approach. Phylogenetic and phylogenomic analyses placed strains P94^T, B95^T and R96^T within the <i>Pseudoalteromonas</i> genus and most closely related to <i>Pseudoalteromonas luteoviolacea</i> and <i>Pseudoalteromonas rubra</i>. Following genome sequencing of strains P94^T, B95^T and R96^T, the average nt identity and <i>in silico</i> DNA-DNA hybridization comparisons with closely related strains resulted in values that fell below species-level cutoffs. Based on a polyphasic characterization and differences in genomic and taxonomic data, strains P94^T, B95^T and R96^T represent novel species, for which the names <i>Pseudoalteromonas ardens</i> sp. nov., <i>Pseudoalteromonas obscura</i> sp. nov. and <i>Pseudoalteromonas umbrosa</i> sp. nov. are proposed. The type strains are R96^T (=DSM 114998^T=LMG 32870^T), P94^T (=DSM 114996^T=LMG 32871^T) and B95^T (=DSM 114997^T=LMG 32872^T), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and identification of a novel yeast species, <i>Hanseniaspora drosophilae</i> sp. nov., from <i>Drosophila</i> in Okinawa, Japan.","authors":"Taisuke Seike, Hiroki Takekata, Keiko Kono, Natsue Sakata, Hazuki Kotani, Chikara Furusawa, Fumio Matsuda","doi":"10.1099/ijsem.0.006661","DOIUrl":"https://doi.org/10.1099/ijsem.0.006661","url":null,"abstract":"<p><p><i>Hanseniaspora</i>, a genus of yeasts in which many species reproduce sexually, has attracted the attention of researchers because of its prevalence in diverse ecological niches. Building on our extensive collection efforts since 2020, three previously unknown yeast strains from wild <i>Drosophila</i> species trapped in ripe bananas in Okinawa, Japan, were isolated. Using a multifaceted approach, including physiological assessments and sequence analysis of the D1/D2 domain of the 26S LSU rRNA gene and the internal transcribed spacer (ITS) region, it was revealed that these strains are novel members of the genus <i>Hanseniaspora</i>. The three strains, JCM 36741^T, JCM 36742 and JCM 36748, had identical sequences in their respective D1/D2 and ITS regions, justifying their classification as a single species. Moreover, the new species exhibited a remarkable degree of sequence divergence from its closest relatives, differing by 7 nucleotide substitutions (1.28%) in the D1/D2 domain, 29 nucleotide substitutions and 4 gaps (4.08%) in the ITS regions. These substantial sequence differences highlight the distinctiveness of this novel species in the genus <i>Hanseniaspora</i>. Further analysis revealed physiological characteristics that distinguished the new species from its closest relative, <i>Hanseniaspora hatyaiensis</i> (nom. inval.). These findings culminated in the proposed name <i>Hanseniaspora drosophilae</i> sp. nov., which recognizes the unique ecological niche within the <i>Drosophila</i> microbiota. By uncovering this novel species, this study not only adds to the growing body of knowledge on yeast diversity but also sheds light on the intricate ecological relationships that shape microbial communities. The implications of this discovery extend beyond taxonomic boundaries, inviting further exploration of the evolutionary dynamics and ecological significance of yeast-fly interactions. We propose accommodating <i>H. drosophilae</i> sp. nov. in the genus <i>Hanseniaspora</i> with JCM 36741^T as the holotype. The MycoBank accession number is MB 853822.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Oceanobacter antarcticus</i> sp. nov., isolated from surface seawater of the Weddell Sea in Antarctica.","authors":"Jinzhu Zhang, Jianning Wang, Yaping Du, Guangyu Li, Xiupian Liu, Panyi Pan, Ziyuan He, Ruolin Cheng, Qiliang Lai, Zongze Shao, Chunming Dong","doi":"10.1099/ijsem.0.006676","DOIUrl":"10.1099/ijsem.0.006676","url":null,"abstract":"<p><p>The poles of the Earth harbour many novel micro-organisms that have not yet been isolated and identified. Here, a Gram-stain-negative, motile, aerobic and rod-shaped bacterial strain, designated as wDCs-4^T, was isolated from surface seawater collected from the Weddell Sea in Antarctica. It grows at 4-40 °C (optimum 10-15 °C), pH 4-8 (optimum 7) and in the presence of 0-4% NaCl (w/v, optimum 0.5-1%). The complete 16S rRNA gene of strain wDCs-4^T had maximum sequence identity with <i>Oceanobacter mangrovi</i> SM2-42^T (97.2%), followed by <i>Thalassolituus oleivorans</i> MIL-1^T (96.5%) and <i>Oceanobacter kriegii</i> 197^T (96.2%). Phylogenetic analyses based on the 16S rRNA gene and genome sequences showed that strain wDCs-4^T was closely clustered with the members of the genus <i>Oceanobacter</i> and formed an independent clade, which could be considered a monophyletic taxon. The average nucleotide identity values between strain wDCs-4^T and the members of the genera <i>Oceanobacter</i> and <i>Thalassolituus</i> were 77.7-78.1 and 77.4-80.5%, respectively. The corresponding digital DNA‒DNA hybridization values are 19.5-20.1 and 20.5-22.4%, respectively. The major fatty acids (>5%) of strain wDCs-4^T comprised summed feature 5 (C_18:0 ante/C_18:2 <i> ω6</i>,9<i>c</i> or C_18:2 <i> ω6</i>,9<i>c</i>/C_18:0 ante) and C_16:0. The predominant respiratory was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, aminolipids and unknown polar lipids. The draft genome size was 4.58 Mbp, with a DNA G+C content of 53.4 mol%. On the basis of polyphasic analyses, strain wDCs-4^T represented a novel species in the genus <i>Oceanobacter</i>, for which the name <i>Oceanobacter antarcticus</i> sp. nov. was proposed. The type strain was wDCs-4^T (=MCCC 1A20726^T=KCTC 8314^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Campylobacter molothri</i> sp. nov. isolated from wild birds.","authors":"William G Miller, Bruno S Lopes, Mary H Chapman, Tina G Williams, Meenakshi Ramjee, Delilah F Wood, James L Bono, Ken J Forbes","doi":"10.1099/ijsem.0.006635","DOIUrl":"10.1099/ijsem.0.006635","url":null,"abstract":"<p><p>Twenty-nine hippuricase-positive <i>Campylobacter</i> strains were isolated from wild birds and river water. Previous characterization using <i>atpA</i> typing indicated that these strains were related to <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> but were most similar to three recently described hippuricase-positive <i>Campylobacter</i> species recovered from zebra finches, i.e. <i>C. aviculae</i>, <i>C. estrildidarum</i> and <i>C. taeniopygiae</i>. Phylogenetic analyses using 330 core genes placed the 29 strains into a clade well separated from the other <i>Campylobacter</i> taxa, indicating that these strains represent a novel <i>Campylobacter</i> species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values were below 70 and 95 %, respectively, thus providing further supporting evidence of a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, spiral cells that are oxidase, catalase and nitrate reductase positive, but urease negative. Genomic analyses indicate that the 29 strains can potentially synthesize very few amino acids <i>de novo</i> and are auxotrophic for many amino acids and cofactors, similar to the species composing the <i>Campylobacter lari</i> group. In addition, these strains encode complete Entner-Doudoroff and Leloir pathways, suggesting that they may possess the ability to utilize both glucose and galactose; these pathways were also identified in the genomes of the zebra finch-associated taxa. The data presented here show that these strains represent a novel species within <i>Campylobacter</i>, for which the name <i>Campylobacter molothri</i> sp. nov. (type strain RM10537^T=LMG 32306^T=CCUG 75331^T) is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11801493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lacrimispora sinapis</i> sp. nov., isolated from pickled potherb mustard (<i>Brassica juncea</i> Coss.).","authors":"Quanlu Ren, Danqi Wang, Jin Han, Zhenmin Liu, Zhengjun Wu","doi":"10.1099/ijsem.0.006675","DOIUrl":"10.1099/ijsem.0.006675","url":null,"abstract":"<p><p>Strain JR3^T was isolated from Chinese pickled potherb mustard (<i>Brassica juncea</i> Coss.) purchased from a local market in Shanghai, China. A polyphasic approach including 16S rRNA gene sequence analysis, average nucleotide identity (ANI) analysis, digital DNA-DNA hybridization (dDDH), determination of G+C content and various phenotypic analyses was employed to characterize strain JR3^T. The bacterium was rod-shaped, Gram-stain-positive, terminal round endospore-forming and catalase-positive. The strain could grow at a wide range of temperatures (20-45 °C) and pH values (6.0-8.0). Optimal growth of strain JR3^T occurred at 35-40 °C and a pH value of 7.0. The strain exhibited growth at salt (NaCl) concentrations of up to 3% (w/v). The G+C content of the genomic DNA was 44.0 mol%. The major fatty acids were C16 : 0, C19 : 0 c9, 10, summed feature 10 (C18 : 1 c11/t9/t6) and C18 : 1 c9. 16S rRNA gene sequencing revealed that strain JR3^T represents a member of the genus <i>Lacrimispora</i>, and it has higher sequence similarity with <i>Lacrimispora amygdalina</i> BR-10^T (=DSM 12857^T) (98.72%), <i>Lacrimispora saccharolyticum</i> WM1^T (98.29%) and <i>Lacrimispora xylanolytica</i> sy1 (98.22%). The dDDH value for strain JR3^T and phylogenetically related species within the genus <i>Lacrimispora</i> ranged from 17.7% to 29.9%. The ANI of strain JR3^T with its closely related taxa was far lower than the threshold (95%-96%) used for species differentiation. Results of phylogenetic, physiological and phenotypic characterization confirmed that strain JR3^T represented a novel species within the genus <i>Lacrimispora</i>, for which the name <i>Lacrimispora sinapis</i> sp. nov. is proposed. The type strain is JR3^T=CCTCC AB 2024044^T=LMG 33655^T.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: <i>Clostridium sediminicola</i> sp. nov., a crude oil aggregation-forming anaerobic bacterium isolated from marine sediment.","authors":"Shiori Hirano, Takeshi Terahara, Ririka Kato, Koji Mori, Moriyuki Hamada, Takamasa Miura, Ryo Matsumoto, Masahiro Hayashi, Takeshi Kobayashi","doi":"10.1099/ijsem.0.006671","DOIUrl":"https://doi.org/10.1099/ijsem.0.006671","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Roseicyclus amphidinii</i> sp. nov., a novel bacterium isolated from the culture of a dinoflagellate <i>Amphidinium carterae</i>.","authors":"Yudong Cui, Xueyi Mo, Zhaobin Huang","doi":"10.1099/ijsem.0.006682","DOIUrl":"https://doi.org/10.1099/ijsem.0.006682","url":null,"abstract":"<p><p>A Gram-negative, rod-shaped and light pink-pigmented bacterial strain, designated Amp-Y-6^T, was isolated from the culture of a dinoflagellate <i>Amphidinium carterae</i> CCMP1314. It can produce bacteriochlorophyll <i>a</i>. The 16S rRNA gene of strain Amp-Y-6^T had the highest sequence similarity with <i>Roseicyclus marinus</i> CCMM001^T of 98.1%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Amp-Y-6^T was affiliated to the genus <i>Roseicyclus</i> and formed a monophyletic clade with <i>R. marinus</i> CCMM001^T and <i>Roseicyclus mahoneyensis</i> ML6^T. The digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity between strain Amp-Y-6^T and the two phylogenetic relatives were 22.7-22.8, 80.4-80.5 and 79.5-80.2%, respectively. The respiratory quinone was Q-10. The major fatty acid composition was summed feature 8 (C_18:1 <i> ω7c</i> and/or C_18:1 <i> ω6c</i>). The draft genome size was 3.8 Mbp with a genomic G+C content of 68.1 mol%. Gene annotation showed that Amp-Y-6^T contained a gene cluster responsible for the C-P degradation, indicating that it had the potential to provide a phosphate source for the dinoflagellate. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain Amp-Y-6^T represents a novel species, for which the name <i>Roseicyclus amphidinii</i> sp. nov. is proposed. The type strain is Amp-Y-6^T (=MCCC 1K08249^T=KCTC 92882^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}