International journal of systematic and evolutionary microbiology最新文献

{"title":"International Committee on Systematics of Prokaryotes: minutes of the open plenary meeting, Tuesday, 22 October 2024, Sesto Fiorentino, Florence, Italy, and via Teams.","authors":"Aharon Oren","doi":"10.1099/ijsem.0.006621","DOIUrl":"10.1099/ijsem.0.006621","url":null,"abstract":"<p><p>A hybrid in-person and online open plenary meeting of the International Committee on Systematics of Prokaryotes (ICSP) was held on 22 October 2024 at the Consiglio Nazionale delle Ricerche, Sesto Fiorentino, Florence, Italy, and via Teams just prior to the IUMS 2024 Congress. To comply with Articles 4(d) and 5(d) (1) of the statutes of the ICSP, the minutes of this meeting are published here.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Desulfosporosinus paludis</i> sp. nov., an acidotolerant sulphate-reducing bacterium isolated from moderately acidic fen soil.","authors":"Stefan Dyksma, Meina Neumann-Schaal, Mathias Müsken, Michael Pester","doi":"10.1099/ijsem.0.006648","DOIUrl":"10.1099/ijsem.0.006648","url":null,"abstract":"<p><p>An obligately anaerobic, spore-forming sulphate-reducing bacterium, strain SB140^T, was isolated from a long-term continuous enrichment culture that was inoculated with peat soil from an acidic fen. Cells were immotile, slightly curved rods that stained Gram-negative. The optimum temperature for growth was 28 °C. Strain SB140^T grew at pH 4.0-7.5 with an optimum pH of 6.0-7.0 using various electron donors and electron acceptors. Yeast extract, sugars, alcohols and organic acids were used as electron donors for sulphate reduction. SB140^T additionally used elemental sulphur and nitrate as electron acceptors but not sulphite, thiosulphate or iron(III) provided as ferrihydrite and fumarate. The 16S rRNA gene sequence placed strain SB140^T in the genus <i>Desulfosporosinus</i> of the phylum <i>Bacillota</i>. The predominant cellular fatty acids were iso-C_15 : 0 (52.6%) and 5,7 C_15 : 2 (19.9%). The draft genome of SB140^T (5.42 Mbp in size) shared 77.4% average nucleotide identity with the closest cultured relatives <i>Desulfosporosinus acididurans</i> M1^T and <i>Desulfosporosinus acidiphilus</i> SJ4^T. On the basis of phenotypic, phylogenetic and genomic characteristics, SB140^T was identified as a novel species within the genus <i>Desulfosporosinus</i>, for which we propose the name <i>Desulfosporosinus paludis</i> sp. nov. The type strain is SB140^T (=DSM 117342^T=JCM 39521^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11771766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparing the 2025 revision of the International Code of Nomenclature of Prokaryotes.","authors":"Aharon Oren, David R Arahal, Henrik Christensen, Markus Göker, Célia M Manaia, Edward R B Moore","doi":"10.1099/ijsem.0.006666","DOIUrl":"10.1099/ijsem.0.006666","url":null,"abstract":"<p><p>The editorial Board of the <i>International Code of Nomenclature of Prokaryotes</i> (ICNP) - the Prokaryotic Code - has compiled already ratified proposed emendations of the ICNP, together with additional editorial changes and clarifications. These were implemented in a draft 2025 revision of the <i>Prokaryotic Code</i>. To comply with Articles 13(b)(4) and 4(d) of the statutes of the International Committee on Systematics of Prokaryotes (ICSP), a public discussion of the document will start on 1 January (or later if required) 2025, to last for 6 months. Here, we present the basis for the revision and the procedure for the discussion. The discussion will be followed by the balloting of the ICSP members.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Jannaschia maritima</i> sp. nov., a novel marine bacterium isolated from the biofilm of concrete breakwater structures.","authors":"Hao Feng, Shuyi Liang, Ruisi Li, Hui Wang, Runlin Cai","doi":"10.1099/ijsem.0.006645","DOIUrl":"https://doi.org/10.1099/ijsem.0.006645","url":null,"abstract":"<p><p>Marine biofilms were newly revealed as a bank of hidden microbial diversity and functional potential. In this study, a Gram-stain-negative, aerobic, oval and non-motile bacterium, designated LMIT008^T, was isolated from the biofilm of concrete breakwater structures located in the coastal area of Shantou, PR China. Strain LMIT008^T was found to grow at salinities of 1-7% NaCl, at pH 5-8 and at temperatures 10-40 °C. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain LMIT008^T belonged to the genus <i>Jannaschia</i> and was closely related to the type strains <i>Jannaschia aquimarina</i> KCTC23555^T (96.03%) and <i>Jannaschia marina</i> SHC-163^T (95.31%). The draft genome size of the strain LMIT008^T was 3.67 Mbp, and the genomic DNA G+C content was 69.83 mol%. The average nucleotide identity value between strain LMIT008^T and the closely related type strain <i>J. aquimarina</i> KCTC23555^T was 74.82%. The predominant cellular fatty acids were identified as summed feature 8 (C_18 : 1 <i> ω</i>7<i>c</i>/C_18 : 1 <i> ω</i>6<i>c</i>) and C_18 : 0, and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Ubiquinone-10 (Q-10) is the sole respiratory quinone. Further, genomic analysis of strain LMIT008^T showed that the strain harbours abundant genes associated with biofilm formation and environmental adaption, explaining the potential strategies for living on concrete breakwater structures. Based on the morphological, phylogenetic, chemotaxonomic and phenotypic characterization, the strain LMIT008^T was considered to represent a novel species in the genus of <i>Jannaschia</i>, for which the name <i>Jannaschia maritima</i> sp. nov. was proposed, with LMIT008^T (=MCCC 1K08854^T=KCTC 8321^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Corynebacterium mayonis</i> sp. nov. isolated from a human blood culture.","authors":"Hamidu T Mohammed, Christina A Koscianski, Taylor E Berent, Garrett G Gordy, Stephen Johnson, Sebastian C Herren, Robin Patel","doi":"10.1099/ijsem.0.006632","DOIUrl":"https://doi.org/10.1099/ijsem.0.006632","url":null,"abstract":"<p><p>A novel <i>Corynebacterium</i> species, strain BD556^T, isolated from blood, was identified at Mayo Clinic, Rochester, MN, USA. After failing definitive identification using MALDI-ToF MS and partial 16S rRNA gene sequencing, BD556^T was characterized using a polyphasic approach, including phenotypic, biochemical and whole-genome sequencing methods. BD556^T was a Gram-positive rod with clubbed ends, facultatively anaerobic, catalase-positive, oxidase-negative and non-motile. Colonies were white, opaque and non-haemolytic with halo-like edges. BD556^T grew at 35 °C in room air, with CO₂ and under anaerobic conditions. BD556^T grew well in 0 and 6% NaCl and weakly in 10% NaCl. The genome size was 2 349 779 bp with a G+C content of 60.39%. Phylogenetic analysis using 16S rRNA gene sequence analysis, average nucleotide identity and digital DNA-DNA hybridization between the genome of BD556^T and the closest type strains from the Type Strain Genome Server database yielded separation values well beyond those required for species delineation. Chemotaxonomic analyses of BD556^T revealed ribose, arabinose and galactose as whole-cell sugars and an A1γ <i>meso</i>-diaminopimelic acid-direct peptidoglycan type. The major cellular fatty acids were C_15 : 0 (21.0%), C_16 : 0 (14.8%), C_17 : 1 ω9c (26.2%), C_17 : 0 (13.3%) and C_18 : 1 ω9c (18.3%). Polar lipids included diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids and phospholipids. BD556^T also contained mycolic acids (32-36 carbons) typical of corynebacteria. The respiratory quinones were dominated by MK-8(H₂) (71.2%) and MK-9(H₂) (25.9%), with smaller amounts of MK-7(H₂) and MK-10(H₂). The results presented support the tenet that BD556^T (=TSD 427^T=NCTC 15078^T) is a novel species for which the name <i>Corynebacterium mayonis</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptacidiphilus alkalitolerans</i> sp. nov., <i>Streptacidiphilus cavernicola</i> sp. nov. and <i>Streptacidiphilus jeojiensis</i> sp. nov. isolated from a cave, and an emended description of the genus <i>Streptacidiphilus</i>.","authors":"Soon Dong Lee, Hong Lim Yang, In Seop Kim","doi":"10.1099/ijsem.0.006652","DOIUrl":"https://doi.org/10.1099/ijsem.0.006652","url":null,"abstract":"<p><p>Six Gram-reaction-positive, strictly aerobic, mycelium-forming actinobacteria were isolated from soils collected from a natural cave in Jeju, Republic of Korea. The isolates produced well-developed, branched, substrate mycelia and white aerial mycelia that differentiated into straight or flexuous chains of smooth-surfaced spores. Cells showed growth at 15-30 °C, pH 3.5-8.0 and 0-1% (w/v) NaCl. Most of the isolates also grew at pH 10.0. The cell-wall peptidoglycan in common contained ll-diaminopimelic acid, galactose, glucose, mannose and rhamnose. The major menaquinone was MK-9(H₆) and MK-9(H₈). The polar lipids in common contained phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid, with the presence of diphosphatidylglycerol and phosphatidylethanolamine in some strains. The predominant fatty acids in common were anteiso-C_15 : 0, iso-C_16 : 0 and C_16 : 0. Strains N1-1^T, N1-3 and N1-12 contained genomes of 8.44-8.77 Mbp, and strains N1-5 and N1-10^T consisted of genomes of 9.00-9.17 Mbp, while strain N8-3^T contained the smallest genome (7.33 Mbp) among the isolates. The genomic DNA G+C contents of the isolates were 71.5-72.2%. Three representatives of the isolates encompassed 16-29 biosynthetic gene clusters predicted to encode for secondary metabolites. The core genome-based phylogenomic tree showed that they formed three distinct clusters within the genus <i>Streptacidiphilu</i>s, with the closest relative, the type strain of <i>Streptacidiphilu</i>s <i>carbonis</i>, which was also supported by 16S rRNA gene phylogeny. The orthologous average nucleotide identity (≤88.2%) and digital DNA-DNA hybridization (≤30.3%) between three representatives of the isolates and members of the genus <i>Streptacidiphilu</i>s and among them supported that the isolates represent three new species of the genus <i>Streptacidiphilu</i>s, for which the names <i>Streptacidiphilus alkalitolerans</i> [type strain, N1-1^T (=KCTC 19224^T=DSM 45080^T)], <i>Streptacidiphilus cavernicola</i> [type strain, N8-3^T (=KCTC 29470^T=DSM 117389^T)] and <i>Streptacidiphilus jeojiensis</i> sp. nov. [type strain, N1-10^T (=KCTC 19257^T=DSM 117391^T=NRRL B-24556^T)] are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Scrofimicrobium appendicitidis</i> sp. nov., isolated from a patient with ruptured appendicitis.","authors":"Hiu-Yin Lao, Annette Y P Wong, Timothy Ting-Leung Ng, Ryan Yik-Lam Wong, Miranda Chong-Yee Yau, Jimmy Yiu-Wing Lam, Gilman Kit-Hang Siu","doi":"10.1099/ijsem.0.006633","DOIUrl":"https://doi.org/10.1099/ijsem.0.006633","url":null,"abstract":"<p><p>A clinical isolate, R131, was isolated from the peritoneal swab of a patient who suffered from ruptured appendicitis with abscess and gangrene in Hong Kong in 2018. Cells are facultatively anaerobic, non-motile, Gram-positive coccobacilli. Colonies were small, grey, semi-translucent, low convex and alpha-haemolytic. The bacterium grew on blood agar but not on Brain Heart Infusion (BHI) and Mueller-Hinton agars. It was negative for catalase, oxidase, indole and aesculin hydrolysis. The initial identification attempts via matrix-assisted laser desorption ionization-time of flight mass spectrometry and 16S rRNA gene sequencing yielded inconclusive results. The 16S rRNA gene analysis showed that R131 shared >99% nucleotide identity with certain uncultured <i>Actinomycetales</i> bacteria. In this retrospective investigation, a complete genome of R131 was constructed, disclosing a DNA G+C content of 64%. Phylogenetic analysis showed that the bacterium was mostly related to <i>Scrofimicrobium canadense</i> WB03_NA08, which was first described in 2020. However, its 16S rRNA gene shared only 94.15% nucleotide identity with that of <i>S. canadense</i> WB03_NA08. Notably, the orthoANI between R131 and <i>S. canadense</i> WB03_NA08 was 67.81%. A pan-genome analysis encompassing R131 and 4 <i>Scrofimicrobium</i> genomes showed 986 core gene clusters shared with the <i>Scrofimicrobium</i> species, along with 601 cloud genes. The average nucleotide identity comparisons within the pan-genome analysis ranged from 59.78 to 62.51% between R131 and the other <i>Scrofimicrobium</i> species. Correspondingly, the dDDH values ranged from 19.20 to 22.30%, while the POCP values spanned from 57.48 to 60.94%. Therefore, a novel species, <i>Scrofimicrobium appendicitidis</i> sp. nov., is proposed. The type strain is R131^T (=JCM 36615^T=LMG 33627^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paenibacillus zeirhizosphaerae</i> sp. nov., isolated from surface of the maize (<i>Zea mays</i>) roots in a horticulture field, Hungary.","authors":"Dalma Márton, Milán Farkas, Anna Bedics, Erika Tóth, András Táncsics, Károly Bóka, Gergely Maróti, Erzsébet Baka, Balázs Kriszt, Mátyás Cserháti","doi":"10.1099/ijsem.0.006628","DOIUrl":"https://doi.org/10.1099/ijsem.0.006628","url":null,"abstract":"<p><p>A novel Gram-stain-positive, rod-shaped, endospore-forming bacterium with peritrichous flagella, designated as P96^T was isolated from the surface of maize roots. Strain P96^T grew optimally at 28 °C, pH 7.0. The strain contained A1γ <i>meso</i>-Dpm-direct in the cell-wall peptidoglycan. The dominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The genome size of strain P96^T was 4.8 Mb, and the G+C content was 50.01%. Phylogenomic analyses based on the whole-genome sequences classified the strain into the genus <i>Paenibacillus</i>. The digital DNA-DNA hybridization and average nucleotide identity relatedness analysis resulted in values below the threshold for prokaryotic species delineation, with the highest values observed for <i>Paenibacillus enshidis</i> KCTC 33519^T (29.4 and 85.2%, respectively). Genotypic data together with phenotypic properties supported the classification of strain P96^T as representative of a novel species of the genus <i>Paenibacillus</i>, for which the name <i>Paenibacillus zeirhizosphaerae</i> sp. nov. is proposed. The type strain is P96^T (=LMG 32802^T = NCAIM B 02678^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudomonas fungipugnans</i> sp. nov., a potently antifungal bacterium isolated from moss.","authors":"Anya Schnyder, Ratchara Kalawong, Leo Eberl, Kirsty Agnoli","doi":"10.1099/ijsem.0.006624","DOIUrl":"https://doi.org/10.1099/ijsem.0.006624","url":null,"abstract":"<p><p>A novel strain, 681, was isolated from a moss sample taken from the Chrutzelried woods in Canton Zürich, Switzerland. The strain showed potent activity against several fungi and oomycetes. It was affiliated to the <i>Pseudomonas</i> genus by 16S rRNA gene sequence phylogeny. Genome sequencing showed a G+C content of 59.9 mol%. The highest average nucleotide identity was 86.63%, and the highest digital DNA-DNA hybridization value was 32.2% (with <i>Pseudomonas nunensis</i> ln5), considerably below the thresholds for species delineation. Multi-locus phylogeny using 81 concatenated sequences indicated that the strain represented a new species within the <i>Pseudomonas mandelii</i> subgroup. This study details its characterization as a new species. The 681 genome was screened <i>in silico</i> using antiSMASH to reveal candidate secondary metabolite clusters for the strong antifungal activity exhibited by 681. Of the 15 clusters identified, we disrupted the seven best and tested for activity against <i>Fusarium solani</i>. The pyrrolnitrin, rhizoxin, novel PKS-NRPS and acaterin gene clusters contributed significantly to the observed antifungal activity. Phenotypic analyses found that strain 681 cells were aerobic, Gram-negative, motile rods (mean length 2.60 µm and mean width of 0.67 µm) with one to three polar flagella. Optimal growth was at 30 °C, but growth also occurred at 8 °C. The pH range was 6-7, with some growth at pH 8. Robust growth occurred at 0-3% (w/v) NaCl and weak growth at 4% (w/v) NaCl, with the optimum at 1% (w/v) NaCl. Strain 681 was oxidase positive, hydrolysed arginine under anaerobic conditions, showed intense fluorescence on King's medium B and produced a hypersensitive response in tobacco leaves. Following our investigations, we propose the designation <i>Pseudomonas fungipugnans</i> sp. nov. for this new species. The type strain, 681, is available from the DSMZ and LMG/BCCM culture collections under the designations DSM 115721 and LMG 33039, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Flavobacterium magnesitis</i> sp. nov. and <i>Flavobacterium zubiriense</i> sp. nov., two novel <i>Flavobacterium</i> species isolated from alkaline magnesite residues.","authors":"Leonor Matos, Lorrie Maccarrio, Ana Paula Chung, Diogo N Proença, Søren Sørensen, Paula V Morais, Romeu Francisco","doi":"10.1099/ijsem.0.006660","DOIUrl":"10.1099/ijsem.0.006660","url":null,"abstract":"<p><p>Three bacterial strains, designated FZUC8N2.13^T, FBOR7N2.3^T and FZUR7N2.5, were isolated from distinct magnesite residues in Spain. Phylogenetic and phylogenomic analysis places them within the genus <i>Flavobacterium</i>. Strains FBOR7N2.3^T and FZUR7N2.5 share 100% of similarity in the 16S rRNA gene sequence, and both are most closely related to <i>Flavobacterium cellulosilyticum</i> AR-3-4^T with which they share 97.5% of 16S rRNA gene similarity. Strain FZUC8N2.13^T forms a distinct lineage most closely related to <i>Flavobacterium lacustre</i> IMCC36792^T with 97.7% 16S rRNA gene similarity. The closest phylogenomic neighbours of these three strains are <i>Flavobacterium flevense</i> DSM 1076^T, '<i>Flavobacterium undicola</i>' BBQ-18^T and <i>Flavobacterium commune</i> PK15^T. The average nucleotide identity and digital DNA-DNA hybridization values between the three strains and closest members of the genus <i>Flavobacterium</i> are below the threshold values of 95% and 70%, respectively. Strains FZUC8N2.13^T, FBOR7N2.3^T and FZUR7N2.5 stain Gram-negative, are rod-shaped and form yellow colonies. Optimum growth occurs at 25 °C and pH 7. The genomic G+C contents are 33.4 mol% for strain FZUC8N2.13^T and 33.2 mol% for strains FBOR7N2.3^T and FZUR7N2.5. The major isoprenoid quinone is menaquinone 6. The major fatty acids are summed feature 3 (C_16 : 1 ω7c and/or C_16 : 1 ω6c) (22.6-31.1%), iso-C_15 : 0 (13.6-16.2 %) and anteiso-C_15 : 0 (8.7-10.5%). The polar lipids consist of two aminolipids, two aminophospholipids and one glycolipid. The phylogenetic, phylogenomic, phenotypic and chemotaxonomic data indicate that FZUC8N2.13^T, FBOR7N2.3^T and FZUR7N2.5 are distinct from the described species of <i>Flavobacterium</i> and should be classified as novel species, for which we propose the names <i>Flavobacterium zubiriense</i> for strain FZUC8N2.13^T (=UCCCB 179^T=CECT 30977^T) and <i>Flavobacterium magnesitis</i> for strains FBOR7N2.3^T (=UCCCB 178^T=CECT 30976^T) and FZUR7N2.5 (=UCCCB 216=CECT 31036).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}