International journal of systematic and evolutionary microbiology最新文献

{"title":"Genomic insights into two new subspecies of <i>Herbaspirillum huttiense</i> strains isolated from diseased foliage in Florida.","authors":"Mousami Poudel, Anuj Sharma, Gerald V Minsavage, Kiersten Fullem, Jose Huguet-Tapia, David J Norman, Erica M Goss, Carrie L Harmon, Jeffrey B Jones","doi":"10.1099/ijsem.0.006597","DOIUrl":"10.1099/ijsem.0.006597","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The genus &lt;i&gt;Herbaspirillum&lt;/i&gt; comprises 13 species, the majority of which are plant colonizers. However, some species are occasionally isolated from environmental sources, including water and polluted soil, while others are opportunistic human pathogens. Four novel bacterial strains were isolated from diseased foliage of tomato and Boston fern in Florida, USA. Phylogenetic analysis based on the 16S rRNA gene sequence placed all strains into the genus &lt;i&gt;Herbaspirillum&lt;/i&gt;. The Gram-negative strains produced opaque, creamy white, mucoid colonies, which is typical of the genus &lt;i&gt;Herbaspirillum&lt;/i&gt;. Biolog biochemical profiling also identified those strains as members of &lt;i&gt;Herbaspirillum&lt;/i&gt;. The strains were subjected to whole-genome sequencing, and their genomes were compared with those of reference strains of &lt;i&gt;Herbaspirillum&lt;/i&gt; spp. using average nucleotide identity (ANI). The two strains isolated from Boston fern shared 99% pairwise ANI, as did the two strains isolated from tomato. Among all reference genomes tested, the novel strains shared the highest ANI to &lt;i&gt;Herbaspirillum huttiense&lt;/i&gt; subsp. &lt;i&gt;huttiense&lt;/i&gt; (G21-1742 and NC 40101, 96.76%; SE1, 97.23%; F1, 97.16%) and to &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;putei&lt;/i&gt;. These values are above the established 95% threshold for species delineation based on ANI. As the ANI between members of the two currently described subspecies of &lt;i&gt;H. huttiense&lt;/i&gt;, i.e. &lt;i&gt;huttiense&lt;/i&gt; and &lt;i&gt;putei&lt;/i&gt;, is also ~97%, it can be inferred that the two groups of novel strains described in this study should be considered as candidates for classification as two new subspecies of &lt;i&gt;H. huttiense&lt;/i&gt;, given that the current &lt;i&gt;H. huttiense&lt;/i&gt; subspecies also have ~97% with the fern and tomato strains. &lt;i&gt;In silico&lt;/i&gt; DNA-DNA hybridization results were consistent with those of ANI; comparison of G21-1742 and NC 40101 with &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;putei&lt;/i&gt; IAM 15032&lt;sup&gt;T&lt;/sup&gt;and &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;huttiense&lt;/i&gt; LMG 2199&lt;sup&gt;T&lt;/sup&gt; produced DNA-DNA hybridization (DDH) values of 66.1 and 73.6 %, respectively. Similarly, SE1 and F1 had DDH values of 68.9 and 68.8% with &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;putei&lt;/i&gt; IAM 15032&lt;sup&gt;T&lt;/sup&gt; and 77.1 and 76.7% with &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;huttiense&lt;/i&gt; LMG 2199&lt;sup&gt;T&lt;/sup&gt;, respectively. The genomes of all novel isolates carry genes involved in plant pathogenesis, including those of the type III secretion system, which are not present in other &lt;i&gt;H. huttiense&lt;/i&gt; strains. Based on genomic and phenotypic data, we conclude that these strains represent the first phytopathogenic subspecies within &lt;i&gt;H. huttiense&lt;/i&gt; and the names proposed are &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;nephrolepidis&lt;/i&gt; for the two strains isolated from &lt;i&gt;Nephrolepis exaltata&lt;/i&gt; (designated strain, G21-1742=LMG 33362=NCPPB 4765) and &lt;i&gt;H. huttiense&lt;/i&gt; subsp. &lt;i&gt;lycopersici&lt;/i&gt; (designated strain, SE1=LMG 3361=NCPPB 4764) for the two strains isolated from &lt;i&gt;Solanum lycopersicu","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11641419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Segatella asaccharophila</i> sp. nov., an anaerobic pectinophile isolated from a two-phase methane fermentation system.","authors":"Tomoki Makiura, Hou-Chia Tseng, Naoshi Fujimoto, Akihiro Ohnishi","doi":"10.1099/ijsem.0.006606","DOIUrl":"10.1099/ijsem.0.006606","url":null,"abstract":"<p><p>An obligately anaerobic, Gram-stain-negative, non-spore-forming and non-motile rod (strain LPYR103-Pre^T) was isolated from a two-phase methane fermentation system. Using 16S rRNA gene sequence-based phylogenetic analysis, strain LPYR103-Pre^T was classified in the genus <i>Segatella</i>. The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization between strain LPYR103-Pre^T and its phylogenetically nearest species <i>- Segatella cerevisiae</i> JCM 30867^T - were 94.4, 77.9 and 23.4%, respectively. The genome size of strain LPYR103-Pre^T was 3 326 733 bp, and its genomic DNA G+C content was 44.05%. The most abundant cellular fatty acid was anteiso-C_15 : 0. The growth of strain LPYR103-Pre^T was stimulated by the addition of pectin, d-galacturonate and d-glucuronate; in contrast, the strain exhibited poor growth in the presence of common sugars, such as glucose. Therefore, strain LPYR103-Pre^T was classified as a pectinophile - a bacterium that shows a preference for pectin and a few related compounds as substrates. Glucose is degraded by type strains of 12 species belonging to the genus <i>Segatella</i>; thus, strain LPYR103-Pre^T is the first described pectinophile belonging to this genus. Strain LPYR103-Pre^T produced succinate and acetate as its major metabolic end products. Based on the differences in the phylogenetic, genomic, physiological and chemotaxonomic characteristics of strain LPYR103-Pre^T and related species, the name <i>Segatella asaccharophila</i> sp. nov. is proposed to accommodate strain LPYR103-Pre^T (= NRIC 0997^T = JCM 37351^T=DSM 118531^T = KCTC 25923^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Actinomadura monticuli</i> sp. nov., isolated from Darangshi Oreum (a volcanic cone), and the reclassification of <i>Actinomadura glauciflava</i> Lu <i>et al</i>. 2003 as a later heterotypic synonym of <i>Actinomadura luteofluorescens</i> (Shinobu 1962) Preobrazhenskaya <i>et al</i>. 1975 (Approved Lists 1980).","authors":"Soon Dong Lee, Hong Lim Yang, In Seop Kim","doi":"10.1099/ijsem.0.006609","DOIUrl":"https://doi.org/10.1099/ijsem.0.006609","url":null,"abstract":"<p><p>Two mycelium-forming actinobacterial strains, designated as DLS-47 and DLS-62^T, were isolated from volcanic ash collected from the surface of a rock on the peak of Darangshi Oreum (a volcanic cone) in Jeju, Republic of Korea, and their taxonomic positions were investigated by a polyphasic approach. Both of the isolates showed growth at 20-42 °C, pH 6.0-9.0 and 0-1% (w/v) NaCl. Furthermore, DLS-47 was found to grow at 45 °C, while strain DLS-62^T grew at pH 10.0 and 3% (w/v) NaCl. The 16S rRNA gene-based phylogeny showed that both of the isolates belonged to the genus <i>Actinomadura</i>; strain DLS-47 was most closely related to <i>Actinomadura chokoriensis</i> DSM 45346^T (100% sequence identity), while strain DLS-62^T formed a tight cluster with <i>Actinomadura bangladeshensis</i> DSM 45347^T (99.5% sequence similarity). Morphological and chemotaxonomic characteristics supported the affiliation of the two isolates to the genus <i>Actinomadura</i>. Phylogenomic analysis based on 92 core gene sequences showed that both of the isolates were most closely related to <i>A. chokoriensis</i> DSM 45346^T. Strain DLS-47 shared 100% of orthologous average nucleotide identity and digital DNA-DNA hybridization values with <i>A. chokoriensis</i> DSM 45346^T, while strain DLS-62^T showed orthologous average nucleotide identity ≤89.8% and digital DNA-DNA hybridization values ≤39.4% with strain DLS-47 and members of the genus <i>Actinomadura</i>. The results of phenotypic assays and comparison of overall genomic relatedness indices support the conclusion that strain DLS-47 (= KACC 23347=DSM 116423) is a strain of <i>A. chokoriensis</i>, while strain DLS-62^T (= KACC 23345^T = DSM 116424^T) represents a new species of the genus <i>Actinomadura</i>, for which the name <i>Actinomadura monticuli</i> sp. nov. is proposed. Also, <i>Actinomadura glauciflava</i> Lu <i>et al.</i> 2003 is reclassified as a later heterotypic synonym of <i>Actinomadura luteofluorescens</i> (Shinobu 1962) Preobrazhenskaya <i>et al</i>. 1975 (Approved Lists 1980) based on analysis of overall genomic relatedness indices and phenotypic similarity.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Yamadazyma oleae</i> f.a. sp. nov. and <i>Yamadazyma molendinolei</i> f.a. sp. nov., two novel ascomycetous yeast species isolated from olive oil mills in Italy, and reassignment of 11 <i>Candida</i> species to the genus <i>Yamadazyma</i>.","authors":"Michele Avesani, Giacomo Zapparoli, Sasitorn Jindamorakot, Savitree Limtong","doi":"10.1099/ijsem.0.006592","DOIUrl":"https://doi.org/10.1099/ijsem.0.006592","url":null,"abstract":"<p><p>Three yeast strains were isolated from an olive paste sample (CBS 18661) and two samples of olive oil (CBS 18662 and CBS 18660) were collected in two different mills in Verona province, Italy. The sequence comparison of the D1/D2 domains of the LSU rRNA gene and the internal transcribed space (ITS) regions indicated that these strains belonged to the genus <i>Yamadazyma</i>. Phylogenetic analysis of concatenated sequences of the ITS regions and the D1/D2 domains indicated that they represented members of two distinct species. Strain CBS 18661 and CBS 18662 showed sequence divergence of 2.7-2.8% (13-15 nucleotide substitutions) in the D1/D2 domains from the holotype of <i>Yamadazyma luoyangensis</i>, <i>Yamadazyma ovata</i>, <i>Yamadazyma siamensis</i> and <i>Candida kanchanaburiensis</i> and 5.9-6.2% identities (36-38 nucleotide substitutions) in the ITS regions from <i>Yamadazyma akitaenisis</i> and <i>Yamadazyma nakazawae</i>. Strain CBS 18660 showed sequence divergence of 0.75-1.1% (4-6 nucleotide substitutions) from <i>Y. ovata, Candida trypodendri</i> and <i>Candida insectorum</i> and 3.2-4.1% (18-24 nucleotide substitutions) in the ITS regions from <i>Yamadazyma dushanensis</i>, <i>Yamadazyma terventina</i>, <i>Yamadazyma mexicana</i> and <i>C. trypodendri</i>. The strains CBS 18661^T (PP391581-PP375117) and CBS 18662 (PP391582-PP375118) are named <i>Yamadazyma oleae</i> f.a. sp. nov., whereas CBS 18660^T (PP149061-PP130150) is assigned as <i>Yamadazyma molendinolei</i> f.a. sp. nov. The MycoBank numbers are MB 854683 and MB 854684, respectively. In addition, 11 <i>Candida</i> species belonging to the <i>Yamadazyma</i> clade have been reassigned to the genus <i>Yamadazyma</i> with the proposal of the following new combinations: <i>Yamadazyma aaseri</i> comb. nov., <i>Yamadazyma conglobata</i> comb. nov., <i>Yamadazyma dendronema</i> comb. nov., <i>Yamadazyma diddensiae</i> comb. nov., <i>Yamadazyma germanica</i> comb. nov., <i>Yamadazyma insectorum</i> comb. nov., <i>Yamadazyma kanchanaburiensis</i> comb. nov., <i>Yamadazyma naeodendra</i> comb. nov., <i>Yamadazyma pseudoaaseri</i> comb. nov., <i>Yamadazyma trypodendri</i> comb. nov. and <i>Yamadazyma vaughaniae</i> comb. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudomonas boreofloridensis</i> sp. nov., and <i>Pseudomonas citrulli</i> sp. nov., isolated from watermelon in Florida.","authors":"Kiersten R Fullem, Michelle P MacLellan, Mousami Poudel, Erica M Goss, Neha Potnis, Gerald V Minsavage, Jeffrey B Jones, Mathews L Paret","doi":"10.1099/ijsem.0.006596","DOIUrl":"https://doi.org/10.1099/ijsem.0.006596","url":null,"abstract":"<p><p>Three fluorescent bacterial strains, K1, K13 and K18, were obtained from watermelon (<i>Citrullus lanatus</i>) foliage symptomatic of bacterial leaf spot of cucurbits in Florida. The strains underwent phenotypic characterization, including LOPAT (levan production, oxidase activity, pectolytic activity on potato, arginine dihydrolase production and hypersensitive response (HR) on both tobacco and tomato) and pathogenicity testing on watermelon and squash seedlings. Whole-genome sequencing of the isolates was performed, and multi-locus sequence analysis (MLSA) utilizing housekeeping genes <i>gltA</i>, <i>rpoD</i>, <i>gapA</i> and <i>gyrB</i> placed the isolates into two distinct clades within the <i>Pseudomonas</i> genus. Average nucleotide identity based on blast (ANIb) was used to compare the isolates to <i>Pseudomonas</i> reference genomes. Using ANIb, the closest relatives to the novel strains were identified as <i>Pseudomonas wayambapalatensis</i> (K1 : 82.58%; K13 : 83.77%) and <i>Pseudomonas kilonensis</i> (K18 : 87.16%), although ANIb values were below the 95% threshold for species delineation. DNA-DNA hybridization (genome-genome distance calculation method), comparison to the online Type Genome Server, Biolog biochemical profiling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were also unable to identify the isolates as any known species of <i>Pseudomonas</i>. Based on the combination of genetic and phenotypic data, we conclude that these isolates represent two novel <i>Pseudomonas</i> species, for which we propose the names <i>Pseudomonas boreofloridensis</i> sp. nov. (K1, K13^T, NCPPB 4759=LMG 33364) and <i>Pseudomonas citrulli</i> sp. nov. (K18^T, NCPPB 4761=LMG 33365). The specific epithet <i>boreofloridensis</i> was chosen for the geographic location of isolation (northern Florida), while <i>citrulli</i> designates the host of origin (<i>C. lanatus</i>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142768771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Amycolatopsis heterodermiae</i> sp. nov. and <i>Actinacidiphila polyblastidii</i> sp. nov., two new actinobacteria isolated from foliose lichens.","authors":"Achiraya Somphong, Wetchasart Polyiam, Kawinnat Buaruang, Chanwit Suriyachadkun, Paranee Sripreechasak, Enjuro Harunari, Yasuhiro Igarashi, Somboon Tanasupawat, Wongsakorn Phongsopitanun","doi":"10.1099/ijsem.0.006598","DOIUrl":"https://doi.org/10.1099/ijsem.0.006598","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Exophiala zingiberis</i> sp. nov., a novel cellulase-producing black yeast-like fungi isolated from ginger in India.","authors":"Rameshwar Avchar, Srushti Shinde, Yaminee Kashid, Parang Gangani, Avinash Sharma","doi":"10.1099/ijsem.0.006614","DOIUrl":"10.1099/ijsem.0.006614","url":null,"abstract":"<p><p>The genus <i>Exophiala</i>, known for its melanized, yeast-like appearance, includes a diverse group of fungi with significant implications across various fields. An isolate representing a novel species was identified within this genus from a ginger tuber from India, based on morphological characteristics and molecular phylogenetic analysis. Phylogenetic analysis of the D1/D2 domain of the 26S LSU rRNA gene, SSU rRNA gene and the internal transcribed spacer (ITS) region confirmed this strain as a new species. It was named <i>Exophiala zingiberis</i> sp. nov. (MycoBank no. MB 855415), with MCC 9960^T designated as the holotype and PYCC 9834 as the isotype. <i>Exophiala zingiberis</i> was positioned as a sister species to a clade that includes <i>Exophiala castellanii</i>, <i>Exophiala mali</i> and three additional species. Phylogenetic analysis indicates that while <i>E. zingiberis</i> shares a common ancestor with these species, it is genetically distinct, underscoring its novel status. The type strain of this novel species differs from <i>Exophiala yuxiensis</i> YMF1.07354^T, the most closely related species, by 19 nucleotide substitutions (3.6% sequence variation) in the D1/D2 region (549 bp compared) and 104 nucleotide substitutions and 44 gaps (27.1% sequence variation) in the ITS region (546 bp compared).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Methanochimaera problematica</i> gen. nov., sp. nov., a novel methanoarchaeon isolated from cold seep sediment and reclassification of <i>Methanomicrobium antiquum</i> as <i>Methanoeremita antiquus</i> gen. nov., comb. nov.","authors":"Yang-Zhi Zhou, Mei-Chin Lai, Yi-Ting You, Shu-Jung Lai, Sue-Yao Wu, Chuan-Chuan Hung, Jiun-Yan Ding, Chao-Jen Shih, Yen-Chi Wu, Wei-Ling Zhang, Sheng-Chung Chen","doi":"10.1099/ijsem.0.006593","DOIUrl":"10.1099/ijsem.0.006593","url":null,"abstract":"<p><p>A hydrogenotrophic methanoarchaeon, designated strain FWC-SCC4^T, was isolated from cold seep sediment of Four-Way Closure Ridge, offshore southwestern Taiwan. Strain FWC-SCC4^Tutilizes H₂/CO₂ or formate, but not acetate, secondary alcohols, methylamines, methanol or ethanol for growth and methane production. Yeast extract is required for growth. The cell morphology is coccoid, with a diameter of 0.8-1.2 µm, and the cell envelope is composed of S-layer protein with M_r about 137.00 kDa. Cells possess multiple flagella and usually occur singly. Strain FWC-SCC4^T grows at a temperature range of 20-40 °C (optimum 37 °C) and a pH range of 5.4-7.2 (optimum 7.0). The NaCl range for growth is 0-0.86 M (optimum 0.09 M). The result of phylogenetic analysis of 16S rRNA gene sequences indicates that the most closely related species are <i>Methanoplanus limicola</i> M3^T and <i>Methanoplanus endosymbiosus</i> MC1^T, with similarities of 95.95 and 95.63%, respectively. The G+C content of the genomic DNA is 40.3 mol%. The overall genome-relatedness indexes (OGRIs) and concatenated ribosomal protein (RBP) phylogenic analysis indicate that strain FWC-SCC4^T is a novel lineage of <i>Methanomicrobiaceae</i>. In addition to strain FWC-SCC4^T, the differences between <i>Methanomicrobium antiquum</i> MobH^T and <i>Methanomicrobium mobile</i> BP^T demonstrated by comparative analysis of genomic G+C content and phylogenetic analysis with non-type strain genomes are enough to support the establishment of a novel genus. In conclusion, strain FWC-SCC4^T (BCRC AR10058^T= NBRC 114595^T) is proposed as the type strain of <i>Methanochimaera problematica</i> gen. nov., sp. nov., and <i>Methanoeremita antiquus</i> gen. nov., comb. nov. is proposed as the new name for <i>Methanomicrobium antiquum</i> MobH^T (=DSM 21220^T= NBRC 104160^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 74, part 9 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006556","DOIUrl":"https://doi.org/10.1099/ijsem.0.006556","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Geodermatophilus maliterrae</i> sp. nov., a member of the <i>Geodermatophilaceae</i> isolated from badland surfaces in the Red Desert, Wyoming, USA.","authors":"Seifeddine Ben Tekaya, Imen Nouioui, Gabryelle May Flores, Meina Neumann-Schaal, Felix Bredoire, Franco Basile, Linda T A van Diepen, Naomi L Ward","doi":"10.1099/ijsem.0.006603","DOIUrl":"10.1099/ijsem.0.006603","url":null,"abstract":"<p><p>A novel Gram-stain-positive, black-pigmented bacterium, designated as WL48A ^T, was isolated from the surface of badland sedimentary rock in the Red Desert of Wyoming and characterized using a polyphasic taxonomic approach. Good growth occurred at 28-32 °C, pH 7-9, and NaCl less than 1% (w/v). Colonies, growing well on International Streptomyces Project media (ISP) 3 and ISP 7, were black and adhering to the agar. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences showed that strain WL48A^T belongs to the family <i>Geodermatophilaceae</i>, forming a distinct sub-branch with <i>Geodermatophilus bullaregiensis</i> DSM 46841^T. The organism showed 16S rRNA gene sequence similarity of 98.8% with <i>G. bullaregiensis</i> DSM 46841^T. Digital DNA-DNA hybridization value between the genome sequences of strain WL48A ^T and <i>G. bullaregiensis</i> DSM 46841^T was 51.8%, below the threshold of 70% for prokaryotic species delineation. The chemotaxonomic investigation revealed the presence of galactose, glucose, mannose, xylose and ribose as well as <i>meso</i>-DAP in the peptidoglycan layer. The polar lipid profiles contained phosphatidylcholine (PC), phosphatidylinositol (PI), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) phosphoglycolipid, phospholipids and an unidentified lipid. The menaquinone profile consisted of MK-9(H₄) (98.2%) and MK-9(H₂) (10.8%). The major fatty acid profile (>15%) comprised iso-C_15 : 0 and iso-C_16 : 0. Based on phenotypic, genetic and genomic data, strain WL48A^T (=DSM 116197^T = NCIMB 15483^T=NCCB 100957^T =ATCC TSD-376^T) merits to be considered as a novel species for which the name <i>Geodermatophilus maliterrae</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142818139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}