International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Niallia tiangongensis</i> sp. nov., isolated from the China Space Station.","authors":"Junxia Yuan, Wende Zhang, Lei Dang, Yan Song, Zhao Yin, Ziwei He, Kanyan Xu, Pei Guo, Hong Yin","doi":"10.1099/ijsem.0.006693","DOIUrl":"https://doi.org/10.1099/ijsem.0.006693","url":null,"abstract":"<p><p>Understanding the characteristics of microbes during long-term space missions is essential for safeguarding the health of astronauts and maintaining the functionality of spacecraft. In this study, a Gram-positive, aerobic, spore-forming, rod-shaped strain JL1B1071^T was isolated from the surface of hardware on the China Space Station. This strain belongs to the genus <i>Niallia</i>, with its closest relative being <i>Niallia circulans</i> ATCC 4513^T. The genome of JL1B1071^T is 5 166 230 bp in size, with a G+C content of 35.6 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between JL1B1071^T and <i>N. circulans</i> ATCC 4513^T are 83.3 and 27.5%, respectively, both below the recommended thresholds for species delineation. The major cellular fatty acids were anteiso-C_15:0 and iso-C_15:0. The major quinone was menaquinone-7 (MK-7). Notably, strain JL1B1071^T demonstrates a unique ability to hydrolyse gelatin, suggesting that it can utilize gelatin as a substrate in nutrient-limited environments. Genomic analysis of JL1B1071^T revealed two conserved signature indels in the GAF domain-containing protein and DNA ligase D protein, which are specific to the genus <i>Niallia</i>. Additionally, structural and functional differences in proteins BshB1 and SplA were identified, which may enhance biofilm formation, oxidative stress response and radiation damage repair, thereby aiding its survival in the space environment. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome annotation, strain JL1B1071^T was considered a novel species within the genus <i>Niallia</i> and is proposed to be named <i>Niallia tiangongensis</i> sp. nov. The type strain is JL1B1071^T (=GDMCC 1.4642=KCTC 43715).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ascoidea xinghuacunensis</i> sp. nov., a novel ascomycetous yeast species from Xinghuacun Fenjiu old workshop, Shanxi province of China.","authors":"Pan Zhen, Hai-Yan Zhu, Ying Han, Lu-Jun Luo, Liang-Chen Guo, Shuang Hu, Yu-Hua Wei, Zhang Wen, Da-Yong Han, Zhong-Wei Lin, Feng-Yan Bai, Pei-Jie Han","doi":"10.1099/ijsem.0.006700","DOIUrl":"10.1099/ijsem.0.006700","url":null,"abstract":"<p><p>Four yeast strains belonging to the ascomycetous yeast genus <i>Ascoidea</i> were isolated from the mixture of remnants of steamed sorghum, Daqu powder and the fermented grain that fell off during transportation on the ground in the workshop which were collected in Xinghuacun Fenjiu old workshop, Shanxi province, PR China. We propose the name <i>Ascoidea xinghuacunensis</i> sp. nov. (holotype: strain CGMCC 2.7787) for the new species, which differs phenotypically from the other three species in this genus: <i>Ascoidea asiatica</i>, <i>Ascoidea rubescens</i> and <i>Ascoidea tarda</i> by its ability to grow at 37 °C. This study represents the first isolation of <i>Ascoidea</i> species within the borders of China and the initial report of the <i>Ascoidea</i> species detailing the isolation from the Baijiu brewing environment.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Limibacterium fermenti</i> gen. nov., sp. nov., a novel member of the family <i>Dysgonomonadaceae</i> isolated from the Chinese strong aroma-type Baijiu fermentation system.","authors":"Yang Gu, Huilin Wang, Xia Zhang, Zhiyun Peng, Jian Su, Dong Zhao, Jia Zheng","doi":"10.1099/ijsem.0.006702","DOIUrl":"https://doi.org/10.1099/ijsem.0.006702","url":null,"abstract":"<p><p>An anaerobic isolate, designated me31^T, was isolated from pit mud in Yibin, Sichuan Province, PR China. Phylogenetic results based on 16S rRNA gene sequence showed that strain me31^T belongs to the family <i>Dysgonomonadaceae</i>, and the most closely related isolated relatives were <i>Seramator thermalis</i> SYSU GA16112^T (93.65%) and <i>Proteiniphilum propionicum</i> JNU-WLY501^T (93.22%). The DNA G+C content was 44.26 mol%. The ANI and AAI values between strain me31^T and the closely related strains were 69.25-71.18% and 69.59-71.15%, respectively. Cells of strain me31^T were Gram-stain-negative, rod-shaped and non-motile. Growth of strain me31^T was observed at 25-37 °C, pH 6.0-8.0 and a salt tolerance range of 0-1.0% (w/v). The predominant respiratory quinone was MK-9. The major fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and C_{17 : 0} 2OH. The polar lipids of strain me31^T were found to consist of phosphatidylethanolamine, three unidentified phospholipids, three unidentified phosphoglycolipids, one unidentified phosphoglycolipid, one unidentified lipid, two unidentified glycolipids and one aminophosphoglycolipid. According to the results of morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic analysis, strain me31^T represents a novel species of a novel genus of the family <i>Dysgonomonadaceae</i>, for which the name <i>Limibacterium fermenti</i> gen. nov., sp. nov. is proposed. The type strain is me31^T (=GDMCC 1.4237^T=KCTC 25756^T=WMCC 10035^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fructilactobacillus frigidiflavus</i> sp. nov., a pigmented species, and <i>Levilactobacillus lettrarii</i> sp. nov., a propionate-producing species isolated from sourdough.","authors":"Vi D Pham, Michael G Gänzle","doi":"10.1099/ijsem.0.006726","DOIUrl":"10.1099/ijsem.0.006726","url":null,"abstract":"<p><p>The sourdough isolates FUA3702, FUA3912 and FUA3913^T, as well as FUA3695^T and FUA3914, could not be identified to known species of the <i>Lactobacillaceae</i>. The 16S rRNA gene sequences of FUA3702 and FUA3913, FUA3695 and FUA3914 were>99% identical to <i>Fructilactobacillus sanfranciscensis</i> and <i>Levilactobacillus lanxiensis</i>, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FUA3913^T when compared to <i>Fl. sanfranciscensis</i> were 83.67 and 26.60%, respectively. In addition, strains FUA3702, FUA3912 and FUA3913^T produce different levels of a yellow C30 carotenoid, but pigmentation has not been described in <i>Fl. sanfranciscensis</i>. The ANI and dDDH values of FUA3695^T and FUA3914 when compared to <i>Lv. langxiensis</i> were 95.22 and 61.20%, respectively. In addition, FUA3695 and FUA3914 convert lactate to 1,2-propanediol and further to propionate. The conversion of lactate to propionate by a single strain has not been documented for any of the species in the <i>Lactobacillaceae</i>. Based on the genomic and physiological characteristics, we proposed the novel species <i>Fructilactobacillus frigidiflavus</i> sp. nov. FUA3913^T (=DSM 118650^T=LMG 33758^T) and <i>Levilactobacillus lettrarii</i> sp. nov. FUA3695^T (=DSM 118651^T=LMG 33759^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11925284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tersicoccus mangrovi</i> sp. nov., a novel actinobacterium from mangrove sediment in Semarang city, Indonesia.","authors":"Mada Triandala Sibero, Zhou-Qing Zheng, Fei-Na Li, Shao-Wei Liu, Xin Xiang, Li Tuo, Cheng-Hang Sun","doi":"10.1099/ijsem.0.006669","DOIUrl":"https://doi.org/10.1099/ijsem.0.006669","url":null,"abstract":"<p><p>A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9^T, was isolated from sediment collected from a mangrove ecosystem in Semarang city, Indonesia. Strain MR15.9^T grew at 4-37 °C (optimum 30 °C), pH 6.0-11.0 (optimum 7.0-8.0), with 0-10% (w/v) NaCl (optimum 0-3%). The genome of strain MR15.9^T was 3.67 Mbp with 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence and genome sequence directed that strain MR15.9^T formed a well-supported clade with <i>Tersicoccus solisilvae</i> CGMCC 1.15480^T and <i>Tersicoccus phoenicis</i> 1P05MA^T and shared the highest similarity to <i>T. solisilvae</i> CGMCC 1.15480^T (98.9% sequence similarity) and <i>T. phoenicis</i> 1P05MA^T (98.6% sequence similarity). However, the comparative genome analysis between strain MR15.9^T and <i>T. solisilvae</i> CGMCC 1.15480^T gave average nt identity value of 85.2% and digital DNA-DNA hybridization value of 29.0%, which were below the statistical threshold for the delineation of the species. Chemotaxonomic data showed that the major fatty acids were anteiso-C_15:0, anteiso-C_17:0 and iso-C_16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified glycolipids and two unidentified phospholipids. MK-8(H₂) and MK-9(H₂) were noted as the predominant respiratory quinones in this strain. The results of polyphasic characterization indicated that strain MR15.9^T represents a novel species of the genus <i>Tersicoccus</i>. The name <i>Tersicoccus mangrovi</i> sp. nov. is proposed, with the type strain MR15.9^T (=MCCC 1K08875^T=KCTC 59105^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Vogesella oryzagri</i> sp. nov., isolated from the rhizosphere of rice and <i>in silico</i> genome mining for the prediction of biosynthetic gene clusters.","authors":"Md Amdadul Huq, Yeon-Ju Kim, M Mizanur Rahman, Md Morshedul Alam, Sathiyaraj Srinivasan, Kwon-Kyoo Kang, Shahina Akter","doi":"10.1099/ijsem.0.006687","DOIUrl":"10.1099/ijsem.0.006687","url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64^T, was isolated from the rhizosphere of rice. The colonies were observed to be creamy white-coloured, smooth, spherical and 0.5-1.1 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAHUQ-64^T was able to grow at 10-40 °C, at pH 5.0-9.5 and in the presence of 0-2.0% NaCl (w/v). The strain was positive for both catalase and oxidase tests. The strain was positive for hydrolysis of l-tyrosine. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus <i>Vogesella</i> and is closely related to <i>Vogesella oryzae</i> L3B39^T (98.6% sequence similarity) and <i>Vogesella facilis</i> TTM-24^T (98.2%). The novel strain MAHUQ-64^T has a draft genome size of 3 827 146 bp (22 contigs), annotated with 3612 protein-coding genes, 74 tRNA and 4 rRNA genes. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-64^T and its closest member <i>V. oryzae</i> L3B39^T were 86.5 and 33.4%, respectively. <i>In silico</i> genome mining revealed several biosynthetic gene clusters in the genome of the novel strain MAHUQ-64^T. The genomic DNA G+C content was determined to be 63.4 mol%. The predominant isoprenoid quinone was ubiquinone-8. The major fatty acids were identified as summed feature 3 (comprising C_{16  :  1} <i>ω</i>7<i>c</i> and/or C_{16  :  1} <i>ω</i>6<i>c</i>) and C_{16  :  0}. Based on dDDH, ANI value, genotypic analysis and chemotaxonomic and physiological data, strain MAHUQ-64^T represents a novel species within the genus <i>Vogesella</i>, for which the name <i>Vogesella oryzagri</i> sp. nov. is proposed, with MAHUQ-64^T (=KACC 22245^T=CGMCC 1.19000^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Tamlana flava</i> sp. nov., isolated from mangrove sediment and genome-based taxonomic analysis of the genus <i>Tamlana</i>.","authors":"Zhaobin Huang, Qiliang Lai, Congjie Dai","doi":"10.1099/ijsem.0.006673","DOIUrl":"10.1099/ijsem.0.006673","url":null,"abstract":"<p><p>A novel yellow-coloured bacterial strain MA10^T was isolated from mangrove sediment and subjected to polyphasic taxonomic identification. Strain MA10^T was Gram-negative, rod-shaped, catalase-positive and oxidase-positive. Carotenoid pigment was present, and flexirubin-type pigment was absent. The 16S rRNA gene of strain MA10^T had the highest sequence similarity with <i>Tamlana crocina</i> HST1-43^T of 94.5%. The genome size was 3.77 Mbp with a genomic G+C content of 36.3%. The phylogenetic analysis of the 16S rRNA gene sequence and whole-genome sequence showed that strain MA10^T belonged to the genus <i>Tamlana</i> of the family <i>Flavobacteriaceae</i> and tightly clustered with <i>T. crocina</i> HST1-43^T. The digital DNA-DNA hybridization value and average nucleotide identity value between strain MA10^T and <i>T. crocina</i> HST1-43^T were 20.4 and 76.7%, respectively. The major menaquinone was MK-6. The major fatty acids (>10 %) were iso-C_15:0 (41.6%) and iso-C_15:1 G (16.8%). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids and three unidentified lipids. Based on the present polyphasic taxonomic study, strain MA10^T was considered to represent a novel species of the genus <i>Tamlana</i>, for which the name <i>Tamlana flava</i> sp. nov. was proposed. The type strain was MA10^T (=MCCC 1K09289^T=KCTC 102321^T). Additionally, the phylogeny of the 16S rRNA gene and whole-genome sequences found that the hitherto described nine species of the genus <i>Tamlana</i> and <i>Algibacter onchidii</i> presented polyphyletic clades, which could be divided into five different genera. The average amino acid identity value of 80% was chosen as the boundary of the five genera. Thus, except for the genus <i>Tamlana</i>, four novel genera named <i>Allotamlana</i> gen. nov., <i>Cognatitamlana</i> gen. nov., <i>Neotamlana</i> gen. nov. and <i>Pseudotamlana</i> gen. nov. were proposed. This study provided valuable taxonomic analysis of the genus <i>Tamlana</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>Actinomadura catellatispora</i> Lu <i>et al.</i> 2003 as a later synonym of <i>Actinomadura livida</i> Lavrova and Preobrazhenskaya 1975 (Approved List 1980) based on whole-genome analysis.","authors":"Guendouz Dif, Nadjette Djemouai, Atika Meklat, Abdelghani Zitouni","doi":"10.1099/ijsem.0.006684","DOIUrl":"https://doi.org/10.1099/ijsem.0.006684","url":null,"abstract":"<p><p>The genus <i>Actinomadura</i> comprises Gram-positive bacteria found in diverse natural environments and is classified in the family <i>Thermomonosporaceae</i> within the order <i>Streptosporangiales</i>. Some species exhibit considerable phylogenetic and taxonomic overlaps, prompting a comprehensive investigation that integrates phylogenetic, phylogenomic and comparative genomic analyses to elucidate the taxonomic status of two <i>Actinomadura</i> species. <i>Actinomadura livida</i> JCM 3387ᵀ and <i>Actinomadura catellatispora</i> DSM 44772^T shared 16S rRNA gene sequence similarity of 99.9% and digital DNA-DNA hybridization values of 99.8%. Moreover, the average nucleotide identity value for the two species was 99.8%, indicating minimal genomic variation between the two strains. Also, the distribution of carbohydrate-active enzymes and biosynthetic gene clusters predicted to code for secondary metabolites between the two species were almost identical. Data of overall genomic similarity support that <i>Actinomadura catellatispora</i> Lu <i>et al.</i> 2003 be a later heterotypic synonym of <i>Actinomadura livida</i> Lavrova and Preobrazhenskaya 1975 (Approved Lists 1980).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of the Nichols strain as the type strain of <i>Treponema pallidum</i>.","authors":"Steven J Norris","doi":"10.1099/ijsem.0.006697","DOIUrl":"10.1099/ijsem.0.006697","url":null,"abstract":"<p><p>In this article, it is proposed that the Nichols strain of <i>Treponema pallidum</i> be established as the type strain. <i>T. pallidum</i> was first identified as the causative agent of syphilis in 1905, and the Nichols strain was isolated in 1912 by inoculation of a rabbit with cerebrospinal fluid from a patient with neurosyphilis. The Nichols strain has been maintained by serial passage in rabbits for over a century, and historically most studies of <i>T. pallidum</i> have been conducted using this strain. In recent years, a procedure for continuous <i>in vitro</i> culture of <i>T. pallidum</i> in a tissue culture system has been developed, making propagation of this spirochaete easier and hence facilitating research. The Nichols strain has >99% DNA homology with a group of organisms that cause syphilis, bejel/endemic syphilis and yaws in humans, a yaws-like disease in primates and spirochaetosis in rabbits and hares. This group is highly similar in terms of their gene and G+C content, genome synteny, cell morphology, natural dependence on mammalian hosts and ability to cause long-term infections; variation occurs in host range, modes of transmission, aptitude for dissemination, manifestations, congenital infection and geographical distribution. Availability of a type strain will aid in the formal acceptance of <i>T. pallidum</i> subspecies first described in 1984 and supported by recent whole-genome analyses of numerous strains from the <i>T. pallidum</i>-related group.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143515797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Flavobacterium fructosi</i> sp. nov., <i>Flavobacterium xylosi</i> sp. nov. and <i>Flavobacterium zhouii</i> sp. nov., three new members of the genus <i>Flavobacterium</i>.","authors":"Dou Han, Lei-Lei Yang, Yu-Hua Xin, Qing Liu","doi":"10.1099/ijsem.0.006694","DOIUrl":"10.1099/ijsem.0.006694","url":null,"abstract":"<p><p>Three novel strains, designated LB3P45^T, LS2P90^T and ZS1P70^T, were isolated from glaciers located on the Tibetan Plateau, PR China. These strains were Gram-stain-negative, aerobic, rod-shaped and yellow or orange coloured. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated that they belong to the genus <i>Flavobacterium</i>. The 16S rRNA gene sequence similarity among the three strains ranged from 97.4 to 98.6%. Strain LB3P45^T showed 98.9% and 98.7% similarity to <i>Flavobacterium urumqiense</i> CGMCC 1.9230^T and <i>Flavobacterium xinjiangense</i> JCM 11314^T, respectively. Strain LS2P90^T displayed 99.0% and 98.7% similarity to <i>F. xinjiangense</i> JCM 11314^T and <i>F. urumqiense</i> CGMCC 1.9230^T. Strain ZS1P70^T had the highest sequence similarity with <i>F. xinjiangense</i> JCM 11314^T (98.0%) and <i>F. urumqiense</i> CGMCC 1.9230^T (97.8%). The average nucleotide identity and digital DNA-DNA hybridization values between these strains and their closest relatives were lower than 93.0% and 47.9%, respectively. All three strains contained summed feature 3 (comprising C_16:1 <i>ω7</i>c and/or C_16:1 <i>ω6</i>c) as the major fatty acids. Based on phenotypic characteristics, phylogenetic analysis and genotypic data, the three novel species are proposed: <i>Flavobacterium fructosi</i> sp. nov. (LB3P45^T=CGMCC 1.11439^T=NBRC 114819^T), <i>Flavobacterium xylosi</i> sp. nov. (LS2P90^T=CGMCC 1.11685^T=NBRC 114823^T) and <i>Flavobacterium zhouii</i> sp. nov. (ZS1P70^T=CGMCC 1.24124^T=NBRC 114829^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143500809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}