International journal of systematic and evolutionary microbiology最新文献

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 74, part 12 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006650","DOIUrl":"https://doi.org/10.1099/ijsem.0.006650","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-evaluation of boundaries of <i>Streptococcus mitis</i> and <i>Streptococcus oralis</i> and demonstration of multiple later synonyms of <i>Streptococcus mitis, Streptococcus oralis</i> and <i>Streptococcus thalassemiae:</i> description of <i>Streptococcus mitis</i> subsp. <i>carlssonii</i> subsp. nov. and emended description of <i>Streptococcus mitis</i>.","authors":"Mogens Kilian, Hans-Christian Slotved, Kurt Fuursted, Adonis D'Mello, Hervé Tettelin","doi":"10.1099/ijsem.0.006704","DOIUrl":"https://doi.org/10.1099/ijsem.0.006704","url":null,"abstract":"<p><p>The commensal species <i>Streptococcus mitis</i> and <i>Streptococcus oralis</i> are genetically diverse to a degree that challenges traditional definitions of species. This causes automatic identification based on DNA sequences or cellular extract profiles problematic. Based on an initial analysis of 266 genomes, we subjected a subset of 100 representative genomes to detailed phylogenetic, pairwise distance and gene pattern analyses. <i>S. mitis</i> and <i>S. oralis</i> constitute a continuum of clones that are genetically unique. To recognize most isolates as separate species is biologically and practically meaningless. We recommend bending the proposed similarity borders to accommodate the biological reality. Accordingly, we conclude that <i>Streptococcus toyakuensis</i>, <i>Streptococcus chosunensis</i>, <i>Streptococcus gwangjuensis</i>, <i>Streptococcus humanilactis</i> and <i>Streptococcus hohhotensis</i> are later heterotypic synonyms of <i>S. mitis</i>. Type strains of effectively but not validly published '<i>Streptococcus shenyangsis</i>', '<i>Streptococcus symci</i>' and '<i>Streptococcus vulneris</i>' belong in <i>S. mitis. Streptococcus parapneumoniae</i> and <i>Streptococcus nakanonensis</i> are later synonyms of <i>Streptococcus thalassemiae. Streptococcus downii</i> is a later synonym of <i>Streptococcus oralis</i> subsp. <i>dentisani</i>, and the type of '<i>Streptococcus halitosis</i>' belongs in <i>Streptococcus oralis</i> subsp. <i>tigurinus</i>. The genome sequence of the type of the recently proposed '<i>Streptococcus bouchesdurhonensis</i>' is based on a mixed culture. Phylogenetic results and the pattern of presence/absence of accessory genes support the distinction of two subspecies of <i>S. mitis</i>, i.e. <i>Sreptococcus mitis</i> subsp<i>. mitis</i> subsp. nov. (type strain is NCTC 12261^T) and <i>Sreptococcus mitis</i> subsp. <i>carlssonii</i> subsp. nov. (type strain is SK608=CCUG 55085^T=LMG 33510^T). The special population structure of the <i>Streptococcus mitis-pneumoniae-pseudopneumoniae-thalassemiae</i> complex renders automated classification of isolates based on average nucleotide identity or digital DNA-DNA hybridization values problematic. As an alternative, for initial taxonomic assignment, we present a whole-genome phylogeny-based method that enables phylogenetic comparison of new isolates in the context of a set of 117 well-characterized reference strains assigned to the Mitis/Sanguinis group.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Qipengyuania profunda</i> sp. nov., isolated from deep seawater of the Amundsen Sea (Antarctica), and reclassification of <i>Qipengyuania aerophila</i> Liu <i>et al</i>. 2022 as a later heterotypic synonym of <i>Qipengyuania pacifica</i> Tareen <i>et al</i>. 2022.","authors":"Bo Min Lee, Jy Young Choi, Su Yeon Lee, Ami Rho, Sukyoung Yun, Won Sang Lee, Byung Cheol Cho, Chung Yeon Hwang","doi":"10.1099/ijsem.0.006713","DOIUrl":"https://doi.org/10.1099/ijsem.0.006713","url":null,"abstract":"<p><p>Gram-stain-negative, aerobic, rod-shaped bacterial strains, designated HL-TH1^T and HL-TH5, were isolated from deep seawater (1127 m depth) of the Amundsen Sea, Antarctica. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strains HL-TH1^T and HL-TH5 were members of the genus <i>Qipengyuania</i> with close relatives <i>Qipengyuania aerophila</i> GH25^T (99.3%), <i>Qipengyuania pacifica</i> NZ-96^T (99.3%), <i>Qipengyuania profundimaris</i> G39^T (99.1%) and <i>Qipengyuania citrea</i> RE35F/1^T (98.8%). The complete genome sequences of strains HL-TH1^T and HL-TH5 comprised a chromosome of 3.2 Mbp and a plasmid of 0.1 Mbp, with DNA G+C content of 64.1%. The whole genome-based comparisons using the average nt identity and digital DNA-DNA hybridization values revealed that both strains belonged to the same genomic species but were clearly discriminated (79.4-85.8% and 21.9-29.3%, respectively) from their close relatives in the genus <i>Qipengyuania</i>. Both strains showed optimal growth at 30 °C, pH 6.5-7.0 and 1.5-2.5% sea salts. The major fatty acids of both strains were C_18:1 <i> ω</i>6<i>c</i> and/or C_18:1 <i> ω</i>7<i>c</i> (37.8-38.4%), C_17:1 <i> ω</i>6<i>c</i> (18.1-19.7%) and C_16:1 <i> ω</i>6<i>c</i> and/or C_16:1 <i> ω</i>7<i>c</i> (12.3-16.7%). The major isoprenoid quinone was ubiquinone-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified lipids. Hence, we propose that strains HL-TH1^T and HL-TH5 are assigned to a novel species belonging to the genus <i>Qipengyuania</i>, for which the name <i>Qipengyuania profunda</i> sp. nov. is proposed. The type strain is HL-TH1^T (=KCCM 90517^T=JCM 36585^T). In addition, <i>Q. aerophila</i> GH25^T and <i>Q. pacifica</i> NZ-96^T were found to be the same species based on a polyphasic approach. Therefore, we propose the reclassification of <i>Q. aerophila</i> as a later heterotypic synonym of <i>Q. pacifica</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Roseovarius conchicola</i> sp. nov. and <i>Aliiroseovarius conchicola</i> sp. nov., isolated from the marine conch <i>Reishia bronni</i>.","authors":"Seung Hee Ha, Min Seo Lee, Mi-Jeong Park, Kae Kyoung Kwon, Jin-Sook Park","doi":"10.1099/ijsem.0.006725","DOIUrl":"https://doi.org/10.1099/ijsem.0.006725","url":null,"abstract":"<p><p>The intertidal zone is an area located between the marine environment and the terrestrial environment and is exposed to various stresses. To investigate the mutualistic relationship between hosts and symbiotic micro-organisms inhabiting the intertidal zone, strains 2305UL8-3^T and 2305UL8-7^T were isolated from <i>Reishia bronni</i>, a species living in the intertidal zone of Ulleungdo Island, South Korea. Both strains are Gram-stain-negative, catalase- and oxidase-positive and facultatively anaerobic. Strains 2305UL8-3^T and 2305UL8-7^T grow optimally at 30.0 °C and 28.0-30.0 °C, respectively, under conditions of pH 8.0 and 3.0 % (w/v) NaCl. They have Q-10 as the primary quinone, and their common main fatty acids are C_16:0 and summed feature 8 (C_18:1 <i>ω</i>7c and/or C_18:1 <i>ω</i>6c). Additionally, their primary polar lipids include phosphatidylcholine and phosphatidylglycerol. The two novel strains have an arsenic reduction pathway that reduces the oxidation state of arsenic and are expected to influence environmental regulation processes through the catabolic sulphate reduction system. Based on these characteristics, they exhibit resistance potential to environmental stresses, specifically arsenic exposure in the intertidal zone, where arsenic contamination is often associated with pollution and tidal fluctuations. Analysing the 16S rRNA gene sequence similarity, strain 2305UL8-3^T shared 96.60 % similarity with <i>Roseovarius faecimaris</i> MME-070^T, while strain 2305UL8-7^T showed 98.13 % similarity with <i>Aliiroseovarius sediminilitoris</i> M-M10^T. Polyphasic analysis revealed that strains 2305UL8-3^T and 2305UL8-7^T should be identified as novel species within the genera <i>Roseovarius</i> and <i>Aliiroseovarius</i>, respectively. Therefore, <i>Roseovarius conchicola</i> sp. nov. with the type strain 2305UL8-3^T (=KCTC 8475^T=MCCC 1K09523^T=JCM 37202^T) and <i>Aliiroseovarius conchicola</i> sp. nov. with the type strain 2305UL8-7^T (=KCTC 8476^T=MCCC 1K09524^T=JCM 37203^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphasic taxonomic description of <i>Streptomyces okerensis</i> sp. nov. and <i>Streptomyces stoeckheimensis</i> sp. nov. and their biotechnological potential.","authors":"Imen Nouioui, Eveline Derr, Alina Zimmermann, Marlen Jando, Gabriele Pötter, Sarah Kirstein, Meina Neumann-Schaal, Cathrin Spröer, Boyke Bunk, Yvonne Mast","doi":"10.1099/ijsem.0.006716","DOIUrl":"10.1099/ijsem.0.006716","url":null,"abstract":"<p><p><i>Streptomyces</i> strains DSM 116494^T and DSM 116496^T were isolated from sediment samples of the River Oker in Braunschweig, Germany, and subjected to a polyphasic taxonomic study and genome mining for specialized secondary metabolites. Phenotypic, genetic and genomic data confirmed the assignment of these strains to the <i>Streptomyces</i> genus. Pairwise 16S rRNA gene sequence similarity values between the strains and validly named <i>Streptomyces</i> species reached 99.5 and 99.7% for strains DSM 116494^T and DSM 116496^T, respectively. Genome-based phylogeny demonstrated that <i>Streptomyces pilosus</i> and <i>Streptomyces griseoflavus</i> species were the close relatives to strain DSM 116494^T, while <i>Streptomyces vinaceus</i> species was the nearest neighbour to strain DSM 116496^T. Digital DNA-DNA hybridization and average nucleotide identity comparisons of the genomic sequence of the strains and their close phylogenomic relatives revealed that values were below the determined threshold of 70 and 95-96% for prokaryotic species demarcation, respectively. The strains were distinguished from their close neighbours based on biochemical, chemotaxonomic and enzymatic data. Given these results, the strains merit being affiliated to novel species within the genus <i>Streptomyces</i>, for which the names <i>Streptomyces okerensis</i> sp. nov. (=OG2.3^T=DSM 116494^T=KCTC 59408^T) and <i>Streptomyces stoeckheimensis</i> sp. nov. (=OG3.14^T=DSM 116496^T=KCTC 59410^T) are proposed. Strains DSM 116494^T and DSM 116496^T harboured several biosynthetic gene clusters encoding potentially novel antimicrobial and anticancer compounds. Crude extracts of strains DSM 116494^T and DSM 116496^T inhibited the growth of Gram-negative bacteria (<i>Escherichia coli</i> ΔtolC, <i>Proteus vulgaris</i>) and a multi-drug-resistant Gram-positive, <i>Staphylococcus aureus</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptomyces solicamelliae</i> sp. nov. and <i>Streptomyces solicathayae</i> sp. nov., isolated from the rhizosphere soil of <i>Camellia oleifera</i> and <i>Cathaya argyrophylla</i>.","authors":"Yaxi Zheng, Ting Tang, Ping Mo, Zhilong Wu, Kaiqin Li, Wansheng Zou, Yun Wang, Baiyuan Li","doi":"10.1099/ijsem.0.006714","DOIUrl":"https://doi.org/10.1099/ijsem.0.006714","url":null,"abstract":"<p><p>Two novel actinobacteria strains, HUAS MG47^T and HUAS YS2^T, were isolated from the rhizosphere soil of <i>Camellia oleifera</i> and <i>Cathaya argyrophylla</i> collected from Changde City, Hunan Province, China. These two strains could form rectiflexible spore chains consisting of short cylindrical spores with smooth surfaces, suggesting that they had typical characteristics of the genus <i>Streptomyces</i>. The cell wall peptidoglycan contained ll-diaminopimelic acid as a diagnostic amino acid. The predominant menaquinones were MK-9 (H₆), MK-9 (H₈) and MK-9 (H₄). The genome sizes of HUAS MG47^T and HUAS YS2^T were 7.35 and 7.88 Mbp with a G+C content of 72.3 and 72.0 mol%, respectively. Phylogenetic analysis based on the 16S rRNA gene and five housekeeping gene sequences (<i>atpD</i>, <i>gyrB</i>, <i>recA</i>, <i>rpoB</i> and <i>trpB</i>) indicated that strains HUAS MG47^T and HUAS YS2^T formed an evolutionary lineage with <i>Streptomyces tritici</i> CGMCC 4.7393^T. However, the values of multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization between these two strains and their closely related relatives indicated that they were clearly different from other known <i>Streptomyces</i> species. Consequently, based on the polyphasic taxonomic study, HUAS MG47^T and HUAS YS2^T represent two novel <i>Streptomyces</i> species for which the names <i>Streptomyces solicamelliae</i> sp. nov. (HUAS MG47^T=MCCC 1K09365^T=JCM 37293^T) and <i>Streptomyces solicathayae</i> sp. nov. (HUAS YS2^T=MCCC 1K08940^T=JCM 36737^T) are proposed, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Christiangramia sediminicola</i> sp. nov., a DNA-hydrolysing bacterium isolated from intertidal sediment.","authors":"Jia-Xuan Wang, Jing Wang, Ning-Hua Liu, Qi-Long Qin, Ping-Yi Li, Xiao-Yan Song, Xiu-Lan Chen, Yu-Zhong Zhang, Xi-Ying Zhang","doi":"10.1099/ijsem.0.006703","DOIUrl":"10.1099/ijsem.0.006703","url":null,"abstract":"<p><p>A Gram-stain-negative, yellow-pigmented, non-flagellated, motile by gliding, aerobic and rod-shaped bacterial strain, designated SM2212^T, was isolated from intertidal sediment of Aoshan Bay in Qingdao, China. The strain grew at 5-35 °C and with 0.5-5.5% NaCl (w/v). It was able to reduce nitrate to nitrite and hydrolyse starch, gelatin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy genes showed that strain SM2212^T belonged to the genus <i>Christiangramia</i> within the family <i>Flavobacteriaceae</i>, sharing the highest 16S rRNA gene sequence similarity with the type strain of <i>Christiangramia echinicola</i> (97.1%) and 96.2-97.0% 16S rRNA gene sequence similarity with those of other known species in the genus. The major cellular fatty acids were summed feature 3 (C_16:1 <i>ω7с</i> and/or C_16:1 <i>ω6с</i>), iso-C_15:0, iso-C_17: 03-OH, summed feature 9 (iso-C_17:1 <i> ω9c</i> and/or 10-methyl C_16:0) and C_16:0. The major polar lipids were phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content of strain SM2212^T was 37.0 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain SM2212^T and type strains of closely related known <i>Christiangramia</i> species were below 22.2 and 79.7%, respectively. Based on the polyphasic taxonomic analysis in this study, strain SM2212^T is considered to represent a novel species in the genus <i>Christiangramia</i>, for which the name <i>Christiangramia sediminicola</i> is proposed. The type strain is SM2212^T (=KCTC 92980^T=MCCC 1K07684^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Rhodococcus parequi</i> sp. nov., a new species isolated from equine farm soil closely related to the pathogen <i>Rhodococcus equi</i>.","authors":"José A Vazquez-Boland, Jorge Val-Calvo, Fabien Duquesne, Francesca Decorosi, Carlo Viti, Sandrine Petry, Mariela Scortti","doi":"10.1099/ijsem.0.006679","DOIUrl":"10.1099/ijsem.0.006679","url":null,"abstract":"<p><p>We present the description of the new species, <i>Rhodococcus parequi</i>, found during phylogenomic investigations of a global collection of strains identified as <i>Rhodococcus</i> (<i>Prescottella</i>) <i>equi</i>. Strain PAM 2766^T was isolated from horse-breeding farm soil in Normandy, France, and was indistinguishable from <i>R. equi</i> based on the usual identification tests. Whole-genome phylogenetic analyses located PAM 2766^T in the same <i>Rhodococcus</i> sublineage as <i>R. equi</i>, together with <i>Rhodococcus agglutinans</i>, <i>Rhodococcus defluvii</i>, <i>Rhodococcus soli</i>, <i>Rhodococcus subtropicus</i>, <i>Rhodococcus spongiicola</i> and <i>Rhodococcus xishaensis</i>. PAM 2766^T is most closely related to, but sufficiently distinct from, <i>R. equi</i> DSM 20307 ^T to be considered a separate species. The average nt identity (ANI) and average aa identity (AAI) values are 88.60% and 92.35, respectively, well below the species cutoff. The PAM 2766^T draft genome is ~5.3 Mb in size with 68.98% G+C mol content. PAM 2766^T is strictly aerobic and non-motile and produces smooth, creamy to buff-coloured colonies very similar to those of <i>R. equi</i>. It phenotypically differs from the latter by the ability to grow at 5 °C, a strongly positive urease test at 24 h and specificities in the carbon and nitrogen source utilization profile as determined by phenotype microarray screens. Our data indicate that PAM 2766^T belongs to a novel species, for which the name <i>Rhodococcus parequi</i> sp. nov. is proposed. <i>R. parequi</i> was avirulent in macrophage infection assays and is assumed to be non-pathogenic. The type strain is PAM 2766^T (=CETC 30995^T=NCTC 14987^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 74, part 11 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006619","DOIUrl":"10.1099/ijsem.0.006619","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143541906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Brevibacterium koreense</i> sp. nov., a moderately halophilic bacterium isolated from jogae-jeotgal, a Korean fermented seafood.","authors":"Sohee Nam, Yujin Kim, Min Ji Lee, Yeon Bee Kim, Jeong Ui Yun, Mi-Ja Jung, Hye Seon Song, Se Hee Lee, Seok-Jun Kim, Tae Woong Whon","doi":"10.1099/ijsem.0.006722","DOIUrl":"https://doi.org/10.1099/ijsem.0.006722","url":null,"abstract":"<p><p>A novel Gram-stain-positive, non-spore-forming, non-motile and rod-shaped bacterium, designated strain CBA3109^T, was isolated from jogae-jeotgal (fermented clam), a traditional Korean fermented seafood. Strain CBA3109^T showed growth at 10-30 °C (optimum, 25 °C) and pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-15% (w/v) NaCl (optimum, 5%). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CBA3109^T belonged to the genus <i>Brevibacterium</i>, with the highest similarities to <i>Brevibacterium aurantiacum</i> NCDO 739^T (98.26%) and <i>B. antiquum</i> VKM Ac-2118^T (98.14%). Strain CBA3109^T contained MK-8(H₂) as the major menaquinone. The cell wall peptidoglycan contained <i>meso</i>-diaminopimelic acid. The major fatty acids (>10%) were anteiso-C_{15 :  0} and anteiso-C_{17 :  0}. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values between strain CBA3109^T and the closest species were 86.2-87.0% and 31.1-33.0 %, respectively. The DNA G+C content of strain CBA3109^T was 62.7%. Based on the morphological, phylogenetic, chemotaxonomic and genotypic data, strain CBA3109^T represents a novel species of the genus <i>Brevibacterium</i>, for which the name <i>Brevibacterium koreense</i> sp. nov. is proposed. The type strain is CBA3109^T (= KACC 23387^T = DSM 117564^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}