Brevibacterium koreense sp. nov., a moderately halophilic bacterium isolated from jogae-jeotgal, a Korean fermented seafood.

Abstract

A novel Gram-stain-positive, non-spore-forming, non-motile and rod-shaped bacterium, designated strain CBA3109^T, was isolated from jogae-jeotgal (fermented clam), a traditional Korean fermented seafood. Strain CBA3109^T showed growth at 10-30 °C (optimum, 25 °C) and pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-15% (w/v) NaCl (optimum, 5%). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CBA3109^T belonged to the genus Brevibacterium, with the highest similarities to Brevibacterium aurantiacum NCDO 739^T (98.26%) and B. antiquum VKM Ac-2118^T (98.14%). Strain CBA3109^T contained MK-8(H₂) as the major menaquinone. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major fatty acids (>10%) were anteiso-C_{15 :  0} and anteiso-C_{17 :  0}. The main polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity and digital DNA-DNA hybridization values between strain CBA3109^T and the closest species were 86.2-87.0% and 31.1-33.0 %, respectively. The DNA G+C content of strain CBA3109^T was 62.7%. Based on the morphological, phylogenetic, chemotaxonomic and genotypic data, strain CBA3109^T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium koreense sp. nov. is proposed. The type strain is CBA3109^T (= KACC 23387^T = DSM 117564^T).