Piscibacter tructae gen. nov., sp. nov., an anaerobic butyrate-producing Fusobacteriaceae bacterium isolated from gut of rainbow trout.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-08-01 DOI:10.1099/ijsem.0.006877

Mio Takeuchi, Kazuhiro Sugahara

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Abstract

A Gram-stain-negative, anaerobic and rod-shaped bacterium was isolated from gut of rainbow trout in Japan. The temperature range for the growth of 9N8^T was 10-30 °C with an optimum at 20 °C. The organism produced acetate and butyrate when grown on AccuDia^™ Gifu Anaerobic Medium broth, Modified. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 9N8^T was related to the genus Fusobacterium (<92.9% similarity) and formed a distinct phylogenetic lineage from existing genera within the family Fusobacteriaceae. Strain 9N8^T has a genome size of 2.60 Mb. The genomic DNA G+C content was 29.4 mol%. Average amino acid identity values between strain 9N8^T and strains in the family Fusobacteriaceae ranged from 39.2 to 57.5%. The major fatty acids of strain 9N8^T were C_14 : 0, C_16 : 1 ω7c and C_16 : 0. On the basis of the morphological, physiological, biochemical and genetic information, a novel genus and species, Piscibacter tructae, is proposed, with 9N8^T (=NBRC 116745^T=DSM 118263^T) as the type strain.

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一种从虹鳟鱼肠道中分离出来的厌氧产丁酸梭杆菌科细菌。

从日本虹鳟鱼肠道中分离出一株革兰氏阴性、厌氧棒状细菌。9N8T的生长温度范围为10 ~ 30℃，20℃时生长最佳。当在改良的AccuDia™Gifu厌氧培养基培养基上生长时，该生物产生乙酸和丁酸盐。16S rRNA基因序列的系统发育分析表明，菌株9N8T与梭杆菌属（Fusobacteriaceae）有亲缘关系。菌株9N8T的基因组大小为2.60 Mb。基因组DNA G+C含量为29.4 mol%。菌株9N8T与梭杆菌科菌株氨基酸同源性平均值为39.2 ~ 57.5%。菌株9N8T的主要脂肪酸为C14: 0、C16: 1 ω7c和C16: 0。根据形态、生理、生化和遗传信息，提出了以9N8T （=NBRC 116745T=DSM 118263T）为模式菌株的新属、新种tructae鱼杆菌（Piscibacter tructae）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.