Oceaniferula spumae sp. nov., a novel Verrucomicrobiota bacterium, isolated from sea foam at Noto Peninsula, Ishikawa, Japan.

Abstract

A Gram-stain-negative, aerobic, rod-shaped bacterium, designated as NT6N^T, was isolated from sea foam collected at the Maura Coast of the Noto Peninsula, Ishikawa, Japan. Strain NT6N^T was able to grow optimally at pH 7.8, 25-30 °C with 3.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NT6N^T belonged to the genus Oceaniferula of the family Verrucomicrobiaceae and was most closely related to Oceaniferula flava corrig. 5K15^T (98.3%) and Oceaniferula marina N1E253^T (95.7%). Furthermore, in silico average nt identity values between strain NT6N^T and its closest related species, O. flava 5K15^T and O. marina N1E253^T, were 76.2% and 70.5%, respectively. The digital DNA-DNA hybridization values for the two comparisons were 20.2% and 19.6%, respectively. The chemotaxonomic characteristics showed that strain NT6N^T had MK-9 and MK-8 as its main components of menaquinone and the main cellular fatty acids were iso-C_14 : 0, anteiso-C_15 : 0, iso-C_16 : 0 and iso-C_12 : 0. The major polar lipids of strain NT6N^T comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The genome of strain NT6N^T had a length of 4.65 Mbp with 52.1 mol% G+C content, containing 3,914 protein-coding genes and 68 RNA genes. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain NT6N^T represents a novel species within the genus Oceaniferula, for which the name Oceaniferula spumae sp. nov. is proposed. The type strain is NT6N^T (=JCM 36658^T=DSM 117338^T=MCCC 1K09726^T).