{"title":"从运城盐湖沉积物中分离到的一种新细菌——salilacus sp. nov.。","authors":"Hui-Ying Yu, Jia-Tan Wei, Chuan-Xu Wang, Zhuo Wang, Ya-Jie Niu, Wen-Jun Li, Bao-Zhu Fang, Hao-Ran Guo, Xin Li","doi":"10.1099/ijsem.0.006846","DOIUrl":null,"url":null,"abstract":"<p><p>Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.0 (optimum pH 7.0-8.0) and with 1-11.0% NaCl (w/v, optimum 3%). Strain YC-516-91<sup>T</sup> was positive for hydrolysis of Tween 80 and starch, but negative for nitrate reduction, urease, H<sub>2</sub>S production, hydrolysis of Tween 40 or gelatin. Phylogenetic analysis based on 16S rRNA gene and genome sequences showed that YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 belonged to the genus <i>Pseudidiomarina</i>. 16S rRNA gene sequence similarity values of YC-516-91<sup>T</sup> to the closest related type strains <i>Pseudidiomarina atlantica</i> MCCC T A10513<sup>T</sup>, <i>Pseudidiomarina terrestris</i> 1APP75-27a<sup>T</sup> and <i>Pseudidiomarina donghaiensis</i> 908033<sup>T</sup> were below 97.99%. Strains YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 have average nucleotide identity (ANI) values of 99-100%, digital DNA-DNA hybridization values of 92.1-97.4% and average amino acid (AAI) values of 99-100%, suggesting that they belong to the same species. These strains are closely related to <i>P. atlantica</i> MCCC 1A10513<sup>T</sup>, with ANI and AAI of around 92-93%, below the species delineation cut-off, thus indicating that they represent a novel species. The genome of strain YC-516-91<sup>T</sup> was 2,723,173 bp, and the genomic DNA G+C content was 50.24 mol%. The only respiratory quinone was Q-8, and the major fatty acids (>10%) were iso-C<sub>17 : 0</sub> and iso-C<sub>15 : 0</sub>. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, glycolipid, unidentified aminolipids and two unidentified polar lipids. Thus, strains YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. Strain YC-516-91<sup>T</sup> is proposed to represent a novel species named <i>Pseudidiomarina salilacus</i> sp. nov., within the genus <i>Pseudidiomarina</i>. The type strain is YC-516-91<sup>T</sup> (=MCCC 1K09529<sup>T</sup>=KCTC 8778<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 7","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Pseudidiomarina salilacus</i> sp. nov., a novel bacterium of the genus <i>Pseudidiomarina</i> isolated from sediment in Yuncheng Salt Lake.\",\"authors\":\"Hui-Ying Yu, Jia-Tan Wei, Chuan-Xu Wang, Zhuo Wang, Ya-Jie Niu, Wen-Jun Li, Bao-Zhu Fang, Hao-Ran Guo, Xin Li\",\"doi\":\"10.1099/ijsem.0.006846\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.0 (optimum pH 7.0-8.0) and with 1-11.0% NaCl (w/v, optimum 3%). Strain YC-516-91<sup>T</sup> was positive for hydrolysis of Tween 80 and starch, but negative for nitrate reduction, urease, H<sub>2</sub>S production, hydrolysis of Tween 40 or gelatin. Phylogenetic analysis based on 16S rRNA gene and genome sequences showed that YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 belonged to the genus <i>Pseudidiomarina</i>. 16S rRNA gene sequence similarity values of YC-516-91<sup>T</sup> to the closest related type strains <i>Pseudidiomarina atlantica</i> MCCC T A10513<sup>T</sup>, <i>Pseudidiomarina terrestris</i> 1APP75-27a<sup>T</sup> and <i>Pseudidiomarina donghaiensis</i> 908033<sup>T</sup> were below 97.99%. Strains YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 have average nucleotide identity (ANI) values of 99-100%, digital DNA-DNA hybridization values of 92.1-97.4% and average amino acid (AAI) values of 99-100%, suggesting that they belong to the same species. These strains are closely related to <i>P. atlantica</i> MCCC 1A10513<sup>T</sup>, with ANI and AAI of around 92-93%, below the species delineation cut-off, thus indicating that they represent a novel species. The genome of strain YC-516-91<sup>T</sup> was 2,723,173 bp, and the genomic DNA G+C content was 50.24 mol%. The only respiratory quinone was Q-8, and the major fatty acids (>10%) were iso-C<sub>17 : 0</sub> and iso-C<sub>15 : 0</sub>. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, glycolipid, unidentified aminolipids and two unidentified polar lipids. Thus, strains YC-516-91<sup>T</sup>, YC-516-48 and YC-516-93 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. Strain YC-516-91<sup>T</sup> is proposed to represent a novel species named <i>Pseudidiomarina salilacus</i> sp. nov., within the genus <i>Pseudidiomarina</i>. The type strain is YC-516-91<sup>T</sup> (=MCCC 1K09529<sup>T</sup>=KCTC 8778<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 7\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006846\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006846","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Pseudidiomarina salilacus sp. nov., a novel bacterium of the genus Pseudidiomarina isolated from sediment in Yuncheng Salt Lake.
Three Gram-stain-negative, aerobic, rod-shaped, yellow-pigmented bacteria, designated YC-516-91T, YC-516-48 and YC-516-93, were isolated from a sediment sample of Yuncheng Salt Lake in China. Growth occurred at 15-42 ℃ (optimum 32-37 ℃), pH 6.0-10.0 (optimum pH 7.0-8.0) and with 1-11.0% NaCl (w/v, optimum 3%). Strain YC-516-91T was positive for hydrolysis of Tween 80 and starch, but negative for nitrate reduction, urease, H2S production, hydrolysis of Tween 40 or gelatin. Phylogenetic analysis based on 16S rRNA gene and genome sequences showed that YC-516-91T, YC-516-48 and YC-516-93 belonged to the genus Pseudidiomarina. 16S rRNA gene sequence similarity values of YC-516-91T to the closest related type strains Pseudidiomarina atlantica MCCC T A10513T, Pseudidiomarina terrestris 1APP75-27aT and Pseudidiomarina donghaiensis 908033T were below 97.99%. Strains YC-516-91T, YC-516-48 and YC-516-93 have average nucleotide identity (ANI) values of 99-100%, digital DNA-DNA hybridization values of 92.1-97.4% and average amino acid (AAI) values of 99-100%, suggesting that they belong to the same species. These strains are closely related to P. atlantica MCCC 1A10513T, with ANI and AAI of around 92-93%, below the species delineation cut-off, thus indicating that they represent a novel species. The genome of strain YC-516-91T was 2,723,173 bp, and the genomic DNA G+C content was 50.24 mol%. The only respiratory quinone was Q-8, and the major fatty acids (>10%) were iso-C17 : 0 and iso-C15 : 0. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, glycolipid, unidentified aminolipids and two unidentified polar lipids. Thus, strains YC-516-91T, YC-516-48 and YC-516-93 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. Strain YC-516-91T is proposed to represent a novel species named Pseudidiomarina salilacus sp. nov., within the genus Pseudidiomarina. The type strain is YC-516-91T (=MCCC 1K09529T=KCTC 8778T).
期刊介绍：
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.
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