International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Chryseobacterium cupriresistens</i> sp. nov., a copper-resistant bacterium isolated from soil contaminated with heavy metals in Chapala Basin, Mexico.","authors":"Ivan Arroyo-Herrera, Ana Laura Reséndiz-Martínez, Brenda Román-Ponce, Joseph Guevara-Luna, Xiaoxia Zhang, Ayixon Sánchez-Reyes, Paulina Estrada-de Los Santos, En Tao Wang, María Soledad Vásquez-Murrieta","doi":"10.1099/ijsem.0.006806","DOIUrl":"10.1099/ijsem.0.006806","url":null,"abstract":"<p><p>A polyphasic taxonomic study was carried out on the bacterial EZn1^T, isolated from heavy-metal-contaminated soil in the Chapala Basin, Mexico. This Gram-negative, aerobic, rod-shaped bacterium formed orange-pigmented colonies, producing the pigment flexirubin. Analysis of the 16S rRNA gene sequence revealed that EZn1^T represents a member of the genus <i>Chryseobacterium</i> in the family <i>Weeksellaceae</i> and is closely related to <i>Chryseobacterium cucumeris</i> GSE06^T (98.3%), <i>Chryseobacterium phosphatilyticum</i> ISE14^T (97.7%) and <i>Chryseobacterium indologenes</i> NBCR 14944^T (97.5%). The average nucleotide identity between the genomes of strain EZn1^T and <i>C. cucumeris</i> GSE06^T was 90.9%, and digital DNA-DNA hybridization showed values of less than 70% with the type strains for the related species. The polar lipids present in the strain included phosphatidylethanolamine, phosphatidylglycerol, glycolipids and unidentified aminoglycolipids, whereas the major cellular fatty acids included iso-C_15 : 0 and iso-C_17 : 0 3-OH. The whole genome of EZn1^T was 5,003,090 bp in length, with a DNA G+C content of 36.7 mol %. The strain EZn1^T showed physiological characteristics different from those of closely related <i>Chryseobacterium</i> species. This strain showed resistance to copper (20 mM), and its genome contained genes that may confer resistance to this metal and other heavy metals (As, Cd, Co, Cr, Hg, K, Mg, Mo, Na and Zn). According to the polyphasic analysis, the strain EZn1^T (=TSD-322^T=CAIM 1954^T) is a novel species that we named <i>Chryseobacterium cupriresistens</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Georgenia wangjunii</i> sp. nov. and <i>Georgenia sunbinii</i> sp. nov. isolated from the faeces of <i>Cygnus atratus</i> in the Qinghai-Tibet Plateau of China.","authors":"Jiajia Ma, Qiang Hou, Hao Zhou, Jing Yang, Caixin Yang, Jinlv Liu, Yaqi Wang, Linglin Cao, Ji Pu, Wei Wang, Dong Jin, Shan Lu, Jianguo Xu","doi":"10.1099/ijsem.0.006762","DOIUrl":"https://doi.org/10.1099/ijsem.0.006762","url":null,"abstract":"<p><p>Four novel, Gram-stain-positive, catalase-positive, oxidase-negative, aerobic and non-motile strains, designated MJ206^T, MJ278, MJ170^T and MJ173, were isolated from the faeces of <i>Cygnus atratus</i> in the Qinghai-Tibet Plateau of China. A comparative analysis of 16S rRNA gene sequences revealed that strain pairs MJ206^T and MJ278, as well as MJ170^T and MJ173, belong to the genus <i>Georgenia</i>. Strain pair MJ206^T/MJ278 is most closely related to '<i>Georgenia faecalis</i>' ZLJ0423 (98.19% 16S rRNA gene sequence similarity), while the strain pair MJ170^T/MJ173 is most related to <i>Georgenia satyanarayanai</i> JC82^T (98.00% 16S rRNA gene sequence similarity). These relationships were further confirmed by phylogenetic analyses based on the 16S rRNA gene and genome sequences. The two type strains (MJ206^T and MJ170^T) have low digital DNA-DNA hybridization values (20.3-23.9%) and average nt identity values (75.3-80.9%) with all available genomes in the genus <i>Georgenia</i>. The DNA G+C contents of strains MJ206^T and MJ170^T were 73.5 and 72.0 mol%, respectively. The predominant cellular fatty acids of both strains (MJ206^T and MJ170^T) were anteiso-C_15 : 0 and anteiso-C_{15:1 A}, but strain MJ206^T has an additional major fatty acid, which is anteiso-C_16 : 0. MK-8 (H₄) was the major menaquinone. The major polar lipids detected in strains MJ206^T and MJ170^T included diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The cell wall aa detected in the two novel species were alanine (Ala), glutamic acid (Glu), glycine (Gly), aspartic acid (Asp) and ornithine acid (Orn); the shared whole cell wall sugars consisted of ribose (Rib), glucose (Glu) and galactose (Gal). All genotypic and phenotypic data indicated that these four strains represented two novel species in the genus <i>Georgenia</i>, for which the names <i>Georgenia wangjunii</i> sp. nov. and <i>Georgenia sunbinii</i> sp. nov. are proposed, respectively. The type strains are MJ206^T (=CGMCC 1.4699^T=JCM 37421^T) and MJ170^T (=CGMCC 1.4698^T=JCM 37420^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Arcobacter iocasae</i> sp. nov., a bacterium isolated from the gill homogenate of the mussel <i>Gigantidas platifrons</i> in a cold seep.","authors":"Huan Zhang, Shi Jin, Hao Wang, Fei Li, Zhaoshan Zhong, Yang Guo, Jia Luo, Ziyun Ye, Chao Lian, Minxiao Wang, Chaolun Li","doi":"10.1099/ijsem.0.006818","DOIUrl":"10.1099/ijsem.0.006818","url":null,"abstract":"<p><p>A novel Gram-negative, oxidase- and catalase-positive, rod-shaped bacterium, designated strain KX21116^T, was isolated from the mussel <i>Gigantidas platifrons</i> collected from a cold seep field in the South China Sea. Strain KX21116^T grew optimally at 28 °C, pH 6.0 with 3% (w/v) NaCl, under aerobic and microaerobic conditions. Its genome size was 3.16 Mb, with a G+C content of 28.4 mol%. The 16S rRNA sequences revealed that strain KX21116^T was closely related to <i>Arcobacter nitrofigilis</i> DSM 7299^T (98.77% gene sequence similarity) and <i>Arcobacter acticola</i> AR-13^T (95.58%). Phylogenetic and phylogenomic analysis revealed that strain KX21116^T clustered with the type species of the genus <i>Arcobacter</i>, with <i>A. nitrofigilis</i> DSM 7299^T as its nearest neighbour. The genomic average nucleotide identity (orthoANI) values between strain KX21116^T and <i>A. nitrofigilis</i> DSM 7299^T were 92.74%, while the <i>in silico</i> DNA-DNA hybridization (GGDC) values of the two strains were 48.8%. The predominant fatty acids are C_16:0, C_16:1 <i> ω7</i>c/C1_6:1 <i> ω6</i>c and C_18:1 <i> ω7</i>c/C1_8:1 <i> ω6</i>c. Based on a comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain KX21116^T represents a novel species of the genus <i>Arcobacter</i>, for which the name <i>Arcobacter iocasae</i> sp. nov. is proposed. The type strain is KX21116^T (=MCCC 1K08505^T=KCTC 92900^T=JCM 35939^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>Moraxella boevrei</i>, <i>M. osloensis</i> and <i>M. atlantae</i>, into the genus <i>Faucicola</i>, and proposal of a new genus within the family <i>Moraxellaceae</i>, <i>Lwoffella lincolnii</i> gen. nov., comb. nov., to accommodate the divergent species <i>Moraxella lincolnii</i>.","authors":"Imed Sbissi, Ikram Kammoun, Haifa El May, Tesnim Bouzidi, Faten Ghodhbane-Gtari, Maher Gtari","doi":"10.1099/ijsem.0.006820","DOIUrl":"https://doi.org/10.1099/ijsem.0.006820","url":null,"abstract":"<p><p>The genus <i>Moraxella</i> (<i>Moraxellaceae</i>, <i>Pseudomonadales</i>) comprises a diverse group of bacteria inhabiting human and animal mucosa, as well as environmental niches such as water, soil and food. While some species are clinically significant pathogens, others play ecological or biotechnological roles. Despite previous taxonomic revisions, <i>Moraxella</i> remains polyphyletic, necessitating a refined classification. In this study, we conducted comprehensive taxogenomic analyses integrating core protein phylogeny, average amino acid identity and the percentage of conserved proteins, along with 16S rRNA similarities and inferred phylogeny. Our results revealed three distinct phylogenetic clusters within <i>Moraxella</i>. The core group, comprising <i>Moraxella lacunata</i> NBRC 102154^T and closely related species, exhibited strong genomic cohesion. A second cluster, consisting of <i>Moraxella boevrei</i> DSM 14165^T, <i>Moraxella osloensis</i> CCUG 350^T and <i>Moraxella atlantae</i> NBRC 14588^T, showed greater genetic affinity to <i>Faucicola mancuniensis</i> GVCNT2^T, supporting their reassignment to the genus <i>Faucicola</i>. The third group, represented by <i>Moraxella lincolnii</i> CCUG 9405^T, was phylogenetically distinct, occupying a basal position relative to <i>Psychrobacter</i>, indicating the need for its classification within a novel genus within the family <i>Moraxellaceae</i>, for which we propose the name <i>Lwoffella lincolnii</i> gen. nov., comb. nov. Phenotypic data compiled from the original published descriptions of the respective species, including fatty acid composition and enzymatic profiles, further corroborated these genomic findings. This study refines <i>Moraxella</i> taxonomy by clarifying genus boundaries and evolutionary relationships, with implications for ecology and clinical microbiology.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudomonas lyxosi</i> sp. nov., <i>Pseudomonas arabinosi</i> sp. nov. and <i>Pseudomonas frigoris</i> sp. nov., isolated from glaciers.","authors":"Chan Zhao, Yu-Hua Xin, Qing Liu","doi":"10.1099/ijsem.0.006799","DOIUrl":"10.1099/ijsem.0.006799","url":null,"abstract":"<p><p>Three Gram-stain-negative, rod-shaped, motile bacterial strains, each with a single polar flagellum, designated LB3P38^T, LT1P18^T and ZB1P45^T, were isolated from glacier samples on the Tibetan Plateau, P.R. China. These strains grew at temperatures ranging from -2 to 32 °C (optimum 25-30 °C) and at pH values of 5.0-11.0 (optimum pH 7.0). The similarities of 16S rRNA gene sequences among these strains ranged from 99.67% to 99.93%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed their affiliation with the genus <i>Pseudomonas</i>. Phylogenomic analysis positioned these strains in close relation to <i>Pseudomonas svalbardensis</i> PMCC 200367^T and <i>Pseudomonas frederiksbergensis</i> LMG 19851^T. Average nucleotide identity values and digital DNA-DNA hybridization values between these strains and other type strains of the genus <i>Pseudomonas</i> were below 94.6% and 63.4%, respectively. The predominant fatty acids identified in these strains were C_16:0, C_17:0 cyclo and summed feature 3 (C_16:1 <i>ω7</i>c and/or C_16:1 <i>ω6</i>c). Based on the combined phenotypic and phylogenetic evidence, each strain is proposed to represent a novel species within the genus <i>Pseudomonas</i>, with the names <i>Pseudomonas lyxosi</i> sp. nov. (type strain=LB3 P38^T=CGMCC 1.11284^T=JCM 37126^T), <i>Pseudomonas arabinosi</i> sp. nov. (type strain=LT1 P18^T=CGMCC 1.11310^T=JCM 37131^T) and <i>Pseudomonas frigoris</i> sp. nov. (type strain=ZB1 P45^T=CGMCC 1.23235^T=JCM 37141^T) proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Microbacterium jiangjiandongii</i> sp. nov. and <i>Microbacterium wangruii</i> sp. nov., two <i>β</i>-cryptoxanthin-producing bacteria isolated from intestinal contents of <i>Marmota himalayana</i>.","authors":"Lin Ye, Xiaorui Liu, Jing Yang, Ji Pu, Caixin Yang, Gui Zhang, Juan Zhou, Yue Liu, Dong Jin, Shan Lu, Jianguo Xu","doi":"10.1099/ijsem.0.006821","DOIUrl":"https://doi.org/10.1099/ijsem.0.006821","url":null,"abstract":"<p><p>Four Gram-stain-positive, facultative anaerobic, yellow-pigmented and short rod-shaped strains, designated zg-Y625^T, zg-Y843, zg-Y1090^T and zg-Y1211, were isolated from the intestinal contents of <i>Marmota himalayana</i> in Qinghai Province, PR China. Strains zg-Y625^T and zg-Y1090^T showed the highest 16S rRNA gene sequence similarities of 99.7% and 99.9% to <i>Microbacterium pullorum</i> DSM 112390^T, respectively, followed by 99.2% and 99.5% to <i>Microbacterium oleivorans</i> JCM 14341^T and 99.1% and 99.0% to <i>Microbacterium paulum</i> LMG 32277^T. Phylogenetic analyses based on 16S rRNA gene sequences and phylogenomic analyses using whole-genome sequences revealed that these four strains belong to the genus <i>Microbacterium</i>, forming two separate clades distinct from all other known <i>Microbacterium</i> species. The genome sizes of strains zg-Y625^T and zg-Y1090^T were 3.26 and 3.07 Mb, respectively, with DNA G+C contents of 70.5 and 70.9 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between each of the novel strains and the available members of the genus <i>Microbacterium</i> were all below the species thresholds. Both type strains contained diphosphatidylglycerol and phosphatidylglycerol as predominant polar lipids with one unidentified glycolipid for zg-Y625^T and two unidentified glycolipids for zg-Y1090^T. The predominant respiratory quinone in zg-Y625^T was MK-13, whilst in zg-Y1090^T, both MK-11 and MK-13 were identified as the major quinones. The major fatty acids (>10%) in strains zg-Y625^T and zg-Y843 were anteiso-C_15 : 0, anteiso-C_17 : 0 and iso-C_16 : 0, whereas for zg-Y1090^T and zg-Y1211, the predominant fatty acids were anteiso-C_15 : 0 and anteiso-C_17 : 0. Based on phenotypic, phylogenetic, genomic and chemotaxonomic data, two novel species in the genus <i>Microbacterium</i> are proposed, namely, <i>Microbacterium jiangjiandongii</i> sp. nov. (zg-Y625^T=GDMCC 1.3931^T=JCM 36203^T) and <i>Microbacterium wangruii</i> sp. nov. (zg-Y1090^T=GDMCC 1.3930^T=JCM 36205^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pedobacter anseongensis</i> sp. nov., <i>Pedobacter immunditicola</i> sp. nov. and <i>Pedobacter superstes</i> sp. nov., three novel species isolated from hydrocarbon- and heavy metal-contaminated soils.","authors":"Jung-Hun Jo, Eun-Young Lee, Ki-Eun Lee, Wan-Taek Im","doi":"10.1099/ijsem.0.006800","DOIUrl":"10.1099/ijsem.0.006800","url":null,"abstract":"<p><p>Three novel, Gram-stain-negative, non-motile, rod-shaped bacterial strains, designated Du54ᵀ, Du274ᵀ and P351ᵀ, were isolated from contaminated environments in Anseong, South Korea. Strains Du54ᵀ and Du274ᵀ originated from landfill soil, whilst P351ᵀ was obtained from parking lot soil. These sites are frequently exposed to pollutants such as petroleum hydrocarbons, heavy metals and industrial runoff, which exert selective pressures on microbial communities. The taxonomic positions of the strains were determined using a polyphasic approach, including 16S rRNA gene sequencing, draft genome analysis and whole-genome-based phylogenetic reconstruction. Phylogenetic trees based on both 16S rRNA genes and core genome sequences (Type Genome Server and AutoMLST) showed that the three isolates belong to the genus <i>Pedobacter</i>. Du54ᵀ exhibited the highest 16S rRNA gene sequence similarity to <i>Pedobacter insulae</i> KCTC 12820ᵀ (98.40%), Du274ᵀ to <i>Pedobacter bambusae</i> KACC 17544ᵀ (98.31%) and P351ᵀ to <i>Pedobacter mongoliensis</i> KCTC 52859ᵀ (97.65%). The draft genomes of Du54ᵀ, Du274ᵀ and P351ᵀ were 4.43, 4.51 and 4.63 Mb in size, with G+C contents of 37.7, 40.6 and 39.2 %, respectively. Average nucleotide identity and digital DNA-DNA hybridization values between the novel strains and their closest relatives ranged from 73.76 to 76.36% and 18.8 to 22.6%, respectively, supporting their delineation as novel species. Genomic annotation revealed 294, 302 and 238 genes in Du54ᵀ, Du274ᵀ and P351ᵀ, respectively, associated with metal resistance, efflux transporters and stress response. All strains lacked flexirubin-type pigments, contained menaquinone-7 as the major respiratory quinone and possessed phosphatidylethanolamine as the dominant polar lipid. The predominant fatty acids included iso-_C16:0 and summed feature 3 (C_16:1 <i> ω</i>7c and/or C_16 : 1 <i> ω</i>6c). Based on these results, the names <i>Pedobacter anseongensis</i> sp. nov., <i>Pedobacter immunditicola</i> sp. nov. and <i>Pedobacter superstes</i> sp. nov. are proposed for strains Du54ᵀ (=KACC 23093ᵀ=LMG 32954ᵀ), Du274ᵀ (=KACC 23094ᵀ=LMG 32955ᵀ) and P351ᵀ (=KACC 23095ᵀ=LMG 32956ᵀ), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based reclassification of the genus <i>Lactococcus</i> and two novel species <i>Pseudolactococcus yaeyamensis</i> gen. nov., sp. nov. and <i>Lactovum odontotermitis</i> sp. nov. isolated from the gut of termites.","authors":"Kota Abe, Masahiro Yuki, Yumiko Imagawa, Atsushi Hisatomi, Moriya Ohkuma, Mitsuo Sakamoto, Satoko Noda","doi":"10.1099/ijsem.0.006803","DOIUrl":"10.1099/ijsem.0.006803","url":null,"abstract":"<p><p>The genus <i>Lactococcus</i> was proposed by Schleifer <i>et al</i>. by separating <i>Lactococcus lactis</i> from the genus <i>Streptococcus</i>. Although the family <i>Streptococcaceae</i> consists of four genera, each genus contains a relatively small number of species, with the exception of the genus <i>Streptococcus</i>, which contains more than 100 species. The genera <i>Lactococcus</i> and <i>Lactovum</i> currently comprise 26 species and a single species, respectively. This study evaluated the taxonomy of the genus <i>Lactococcus</i> based on the 16S rRNA gene phylogeny, core-genome phylogeny and (conserved) pairwise average amino acid identity. These evaluations clearly indicated that the genus <i>Lactococcus</i> could be divided into two genus-level clusters, and we propose to reclassify this genus into two; the authentic <i>Lactococcus</i>, which includes the <i>L. lactis</i> group, and a novel genus for which the name <i>Pseudolactococcus</i> is proposed. Three lactic acid bacterial strains, RyT2^T, OfM1^T and OfM2, were isolated from the gut of termites in Okinawa, Japan. Based on the combination of genetic and phenotypic data, we conclude that these isolates represent two novel species of the genera <i>Pseudolactococcus</i> and <i>Lactovum</i>, respectively, for which we propose the names <i>Pseudolactococcus yaeyamensis</i> sp. nov. (RyT2^T=JCM 36015^T=DSM 118067^T) and <i>Lactovum odontotermitis</i> sp. nov. (OfM1^T=JCM 34431^T=DSM 118066^T, OfM2=JCM 34432), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Amycolatopsis ponsaeliensis</i> sp. nov., a novel endophytic actinobacterium isolated from the root nodules of <i>Alnus glutinosa</i>.","authors":"Ryan Michael Thompson, Edward M Fox, Georgios Koutsidis, Maria Del Carmen Montero-Calasanz","doi":"10.1099/ijsem.0.006810","DOIUrl":"https://doi.org/10.1099/ijsem.0.006810","url":null,"abstract":"<p><p>The root nodules of <i>Alnus glutinosa</i> remain a relatively understudied niche, with poorly described associated microbial communities. In this study, the isolate RTGN1^T was recovered from root nodules collected from Gateshead, UK, and was identified as belonging to <i>Amycolatopsis</i> based on 16S rRNA gene similarity and phylogenomic placement. This isolate was polyphasically characterized, displaying the ability to grow between 12 and 28 °C and pH 6 and 8 and exhibiting the genes necessary to produce the polar lipids phosphatidylethanolamine and phosphatidylglycerophosphate, alongside DL-type peptidoglycan, which are diagnostic of <i>Amycolatopsis</i>. Overall genomic relatedness index values were below the cut-off value for delineating a novel species. As such, it is proposed that RTGN1^T be recognized as the type strain (=CECT 30870^T=CABI 507287^T) of <i>Amycolatopsis ponsaeliensis</i> sp. nov. The RTGN1^T isolate was screened using <i>in silico</i> and <i>in vitro</i> methods and was found to possess a number of genes and pathways related to secondary metabolite production and plant growth promotion. Such genes may serve as an avenue of future study regarding biotechnological potential and use as a bioinoculant to increase phytoremediation efficiency.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Chryseobacterium gossypii</i> sp. nov., a bacterium isolated from long-term continuous cropping cotton fields.","authors":"Mila Mulati, Delong Kong, Lili Chai, Yuanyang Yi, Zhidong Zhang, Wei Zhang","doi":"10.1099/ijsem.0.006817","DOIUrl":"https://doi.org/10.1099/ijsem.0.006817","url":null,"abstract":"<p><p>A novel bacterium, designated 1RM2^T, was isolated from Xinjiang Province, north-west PR China. This strain could grow under conditions of 20-45 °C, pH 5.0-10.0 and 0-10% (w/v) NaCl. The species with the highest similarity of 16S rRNA gene sequences to strain 1RM2^T were strain Chryseobacterium bernardetii NCTC 13530^T (97.1%) and <i>Chryseobacterium daecheongense</i> DSM 15235^T (96.9%). The draft genome sequence G+C content of strain 1RM2^T was 39.5 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain 1RM2^T and the two closest neighbours were 78.6%, 77.6% and 21.8%, 21.2%, respectively. The main fatty acids of strain 1RM2^T were iso-C_15:0, iso-C_17:0 3-OH, summed feature 3 (C_16:1 <i> ω</i>6<i>c</i> and/or C_16:1 <i> ω</i>7<i>c</i>) and summed feature 9 (C_16:0 10-methyl and/or iso-C_17:1 <i> ω</i>9<i>c</i>). The main isoprenoid quinone was menaquinone-6 and polar lipids were phosphatidylethanolamine, unidentified amino phospholipids and unidentified lipids. Based on phenotypic characteristics and genotype analysis, strain 1RM2^T is a new species of the genus <i>Chryseobacterium</i> and is proposed to be named <i>Chryseobacterium gossypii</i> sp. nov. (=GDMCC 1.4437^T=KCTC 102275^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}