International journal of systematic and evolutionary microbiology最新文献

{"title":"Description of <i>Bernardella vulneris</i> gen. nov., sp. nov., assignable to the family <i>Aerococcaceae</i>, isolated from human clinical samples.","authors":"Anne-Marie Bernier, Dmytro Lyubashenko, Ana Luisa Pacheco, Mark Unger, Marc-Christian Domingo, Jennifer Tanner","doi":"10.1099/ijsem.0.007180","DOIUrl":"10.1099/ijsem.0.007180","url":null,"abstract":"<p><p>Eleven novel Gram-stain-positive, catalase- and oxidase-negative coccal bacterial isolates recovered mostly from the wounds of patients located in three Canadian provinces were extensively studied. Phenotypic analysis and phylogenetic analysis based on both the 16S rRNA gene and whole-genome sequence place them in the family <i>Aerococcaceae</i> and differentiate them from genera in the closest families <i>Enterococcaceae</i> and <i>Carnobacteriaceae</i> in the order <i>Lactobacillales</i>. The G+C content of the isolates was on average 42.15 mol% with genome sizes ranging from 1.87 to 2.04 Mb. The average nucleotide identity and average amino acid identity values to other members of the family <i>Aerococcaceae</i> were below 69 and 62%, respectively, placing the isolates into a new genera. Phosphatidylserine is predicted to be synthesized based on the presence of the gene phosphatidylserine synthase in the annotated genome (E.C. 2.7.8.8). <i>In silico</i> analysis predicts the presence of l-lysine as the main diamino acid in the peptidoglycans. Major cellular fatty acids included C_16:0, C_18:0 and C_18:1 <i>ω</i>9c. These 11 clinical strains were ultimately deemed to represent a novel genus and species, for which the name <i>Bernardella</i> gen. nov. and <i>Bernardella vulneris</i> sp. nov. is proposed and the type strain is NML130460^T (=LMG 32739^T=NCTC 14840^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13472200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148723768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Yersinia antarctica</i> sp. nov., isolated from an Antarctic insect - expanding the genus into polar ecosystems.","authors":"Anne-Sophie Le Guern, Matan Shelomi, Tetiana Bahlai, Laura Guichard, Carine Martins, Javier Pizarro-Cerda, Cyril Savin","doi":"10.1099/ijsem.0.007268","DOIUrl":"10.1099/ijsem.0.007268","url":null,"abstract":"<p><p>Five Gram-negative strains, designated BI01^T, BI02, BI1211, BI4D and BI67 isolated from the gut of the Antarctic endemic insect <i>Belgica antarctica</i>, were characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that the bacterial strains belonged to the genus <i>Yersinia</i>. A cgMLST-based phylogeny based on 500 concatenated core genes strongly supported a clade encompassing the 5 strains that was distinct from the currently recognized 27 species of the genus <i>Yersinia</i>. Average nucleotide identity (ANI) among the five strains consistently exceeded 99%, but comparisons to their closest relative, <i>Yersinia intermedia</i>, yielded an ANI of 93% and a digital DNA-DNA hybridization value of 51.74. Metabolic profiling further differentiated the five strains from other closely related species within the genus <i>Yersinia</i>, notably with respect to l-fucose and potassium 2-ketogluconate fermentation, acetoin production and an absence of pyrazinamidase activity. Interestingly, three of the five strains exhibited resistance to <i>β</i>-lactams (amoxicillin and amoxicillin/clavulanate) and first- and second-generation cephalosporins (cefalexin and cefoxitin) associated with the presence of the <i>ampC</i> gene, encoding a class C beta-lactamase, in their genome. None of the five isolates harbour the classic virulence determinants of enteropathogenic <i>Yersinia</i>. The DNA G+C content of the five strains is 47.8 mol%. Based on these results, they represent a novel species of the genus <i>Yersinia</i>, for which the name <i>Yersinia antarctica</i> sp. nov. is proposed. Notably, this represents the first <i>Yersinia</i> species reported from Antarctic terrestrial invertebrates. The type strain is BI01^T (=CIP 112622^T=DSM 121309^T). The distribution of this species is not limited to Antarctica, as few publicly available genomes indicate its presence in continental Russia and the Russian Arctic, with isolates recovered from rodents, polar bears and birds.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148701227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Komagataeibacter ossonobensis</i> sp. nov., a novel bacterial cellulose producer isolated from an artisanal Mediterranean acetification.","authors":"Diogo Neves Proença, Djenabu Candé, Célia Quintas, Bruno Medronho, Anabela Romano","doi":"10.1099/ijsem.0.007261","DOIUrl":"https://doi.org/10.1099/ijsem.0.007261","url":null,"abstract":"<p><p>Bacterial strain CS8^T, a novel bacterial cellulose producer, was isolated from a 'mother of vinegar' during an artisanal acetification process in Faro, Portugal and characterized. Strain CS8^T stained Gram-negative and formed rod-shaped cells that grew optimally at 30 °C and at pH 5.5-6.5. Phylogenetic analyses based on the 16S rRNA gene placed strain CS8^T within the genus <i>Komagataeibacter</i> in a distinct lineage, showing the highest sequence similarity to <i>Komagataeibacter diospyri</i> (99.86%), <i>Komagataeibacter piraceti</i> (99.80%), <i>Komagataeibacter europaeus</i> (99.80%), <i>Komagataeibacter sucrofermentans</i> (99.79%) and <i>Komagataeibacter nataicola</i> (99.72%). Genomic analysis revealed that overall genome relatedness index values of CS8ᵀ with <i>K. europaeus</i> LMG 18890ᵀ, <i>Komagataeibacter swingsii</i> LMG 22125ᵀ and <i>K. diospyri</i> MSKU 9^T were 93.7% average nucleotide identity (ANI) and 53.3% digital DNA-DNA hybridization (dDDH), 91.7% ANI and 44.7% dDDH and 90.4 and 40.4%, respectively, which are below the species delineation thresholds. The genomic DNA G+C content of CS8ᵀ is 60.7 mol%. The draft genome sequence of strain CS8^T comprises 4,020,094 bases with a 225-fold mapped genome coverage. The assembled genome consists of 131 large contigs with more than 500 bp and the genome encodes 4,229 putative coding sequences. Phylogenomic analysis based on 823 core genes placed strain CS8ᵀ in a distinct clade within the genus <i>Komagataeibacter</i>, clustering closely with <i>Komagataeibacter europaeus</i> LMG 18890ᵀ. Analysis of the CS8^T genome revealed the presence of the terpene, hserlactone, triceptide and methanobactin biosynthetic gene clusters. The strain harbours a complete <i>bcs</i> gene operon and efficiently produces bacterial cellulose from glucose. The phylogenomic, phylogenetic, phenotypic and chemotaxonomic data showed that the strain CS8^T (=UCCCB 286^T=CECT 31419^T) represents the type of a novel species, for which we propose the name <i>Komagataeibacter ossonobensis</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Allolecanicillium elzae</i> gen. et sp. nov. (Cordycipitaceae): revised taxonomy with new combinations, its entomopathogenicity and mass production.","authors":"Ana Carla da Silva Santos, Athaline Gonçalves Diniz, Sheila Karine Belo Pedroso, Renan do Nascimento Barbosa, Roger Fagner Ribeiro Melo, Neiva Tinti de Oliveira, Patricia Vieira Tiago","doi":"10.1099/ijsem.0.007256","DOIUrl":"10.1099/ijsem.0.007256","url":null,"abstract":"<p><p>In a previous study, an isolate identified as <i>Akanthomyces sensu lato</i>, obtained from the citrus blackfly <i>Aleurocanthus woglumi</i> in Brazil, was shown to be pathogenic towards its host. Phylogenetic evidence indicated that this isolate belongs to a new taxon. This study aimed to describe this new taxon based on both morphological and molecular phylogenetic markers, to assess its potential for the biological control of the conehead termite (<i>Nasutitermes corniger</i>) and the citrus orthezia (<i>Praelongorthezia praelonga</i>) and to evaluate substrates for the mass production of this fungus. Phylogenetic analysis based on <i>TEF1</i>, <i>RPB1, RPB2</i> and ITS partial sequences demonstrated that this new species is related to <i>Lecanicillium aranearum, Lecanicillium araneicola</i>, <i>Lecanicillium rasoulzarei</i> and <i>Lecanicillium spenceae</i>. However, these species form a clade that corresponds to neither <i>Akanthomyces sensu stricto</i> nor <i>Lecanicillium sensu stricto</i>. Therefore, a new genus <i>Allolecanicillium</i> is proposed here to accommodate <i>Allolecanicillium elzae</i> sp. nov., <i>Allolecanicillium aranearum</i> comb. nov., <i>Allolecanicillium araneicola</i> comb. nov., <i>Allolecanicillium rasoulzarei</i> comb. nov. and <i>Allolecanicillium spenceae</i> comb. nov. These species produce dense white to cream mycelia and simple or branched conidiophores bearing cylindrical to fusoid conidia. The sexual morph is known only for the species <i>A. aranearum</i>. Sporulation of <i>A. elzae</i> on vegetable substrates ranged from 1.46×10⁸ conidia/g of substrate (white rice) to 6.42×10⁸ conidia/g of substrate (sugarcane bagasse). Germination rates did not differ significantly among substrates. Despite being pathogenic to <i>P. praelonga</i> and <i>N. corniger</i>, this fungus showed relatively low virulence against these insects under the conditions tested. This research expands our knowledge of the diversity of entomopathogenic fungi, their host spectrum and alternatives for their production, aiming to foster the recognition of these organisms as sustainable biological resources.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13460516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Actinopolyspora sabaoui</i> sp. nov., a novel halophilic actinobacterium isolated from Ghardaïa soil, Algeria.","authors":"Rafika Saker, Mónica Majo-Cuervo, Noureddine Bouras, Lorena Carro, Khaoula Bouznada, Rafik Ould Taleb, Meina Neumann-Schaal, Sarah Kirstein, Atika Meklat, Esther Menéndez, Imen Nouioui","doi":"10.1099/ijsem.0.007269","DOIUrl":"https://doi.org/10.1099/ijsem.0.007269","url":null,"abstract":"<p><p>A novel halophilic actinobacterium, designated H202^T, was isolated from a Saharan soil in the Ahbès region (Béni-Isguen, Ghardaïa province, South Algeria), and its taxonomic position was determined by a genome-based polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the strain H202^T belonged to the genus <i>Actinopolyspora</i> and showed the highest sequence similarity to <i>Actinopolyspora mzabensis</i> DSM 45460^T (99.46%). Comparative genomic analysis based on digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) showed values below the recommended species cut-off thresholds of 70% (dDDH) and 95-96% (ANI and AAI), suggesting that the strain H202^T represents a novel species. This strain exhibits the typical characteristics of the genus <i>Actinopolyspora</i>. The cell-wall hydrolysates contained <i>meso</i>-diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylcholine was identified as the diagnostic phospholipid, and the predominant menaquinones were MK-9(H₄) and MK-10(H₄). The major fatty acids (≥10%) were <i>anteiso</i>-C_17:0, <i>iso</i>-C_15:0, <i>iso</i>-C_16:0 and <i>anteiso</i>-C_15:0. The genome size of strain H202^T was estimated to be 5.2 Mbp, with a G+C content of 67.5%. Based on these findings, strain H202^T is proposed as a representative of a novel species within the genus <i>Actinopolyspora</i>, named <i>Actinopolyspora sabaoui</i> sp. nov. The type strain is H202^T (=DSM 46671^T=CECT 31287^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Clonostachys kasetsartana</i> sp. nov., a novel epiphytic fungus isolated from the mangrove leaves in Thailand.","authors":"Ananya Sahathippayakul, Vassana Supapongsri, Nattakorn Kuncharoen, Nakarin Suwannarach, Piyangkun Lueangjaroenkit","doi":"10.1099/ijsem.0.007273","DOIUrl":"https://doi.org/10.1099/ijsem.0.007273","url":null,"abstract":"<p><p>Two fungal isolates, DMKU-RG2M15ᵀ and DMKU-RG2P11, were isolated from the phylloplane of healthy leaves of the mangrove tree <i>Ceriops tagal</i> (Perr.) C.B. Rob., collected in Chanthaburi Province, Thailand. Their taxonomic placement was investigated using a polyphasic approach based on morphological, physiological and multilocus phylogenetic analyses. Colonies produced primary conidiophores with verticillate branching, bearing hyaline, smooth-walled, oval to ellipsoidal conidia. Phylogenetic analyses based on a combined dataset of the internal transcribed spacer region, the large subunit rRNA gene, translation elongation factor 1-α, β-tubulin and RNA polymerase II second-largest subunit resolved the two isolates as a distinct and well-supported monophyletic lineage within the genus <i>Clonostachys</i>. Phylogenetic analyses showed that the two isolates are closely related to <i>Clonostachys mangrovei</i>, <i>Clonostachys viticola</i> and <i>Clonostachys reniformis</i>. Morphological and physiological comparisons further distinguished the isolates from their closest relatives. The congruence between phylogenetic and phenotypic data supports the recognition of the two isolates as representing a distinct species. Accordingly, both strains are proposed as a novel species of <i>Clonostachys</i>, for which the name <i>Clonostachys kasetsartana</i> sp. nov. is introduced.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pseudoneobacillus rhizolycopersici</i> sp. nov., a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.","authors":"Shengfeng Pan, Zengwei Feng, Meng Chen, Xiaolin Xie, Qing Yao, Ming-Rong Deng, Honghui Zhu","doi":"10.1099/ijsem.0.007272","DOIUrl":"https://doi.org/10.1099/ijsem.0.007272","url":null,"abstract":"<p><p>A novel Gram-stain-positive, rod-shaped bacterium with peritrichous flagella, designated C159^T, was isolated from tomato rhizosphere soil. Growth of strain C159^T occurred at 4-45 °C, pH 6.0-10.0 and in the presence of 0-5% NaCl. Based on 16S rRNA gene sequence analysis, strain C159^T belongs to the genus <i>Pseudoneobacillus</i> and has a sequence similarity of 98.70% with <i>Pseudoneobacillus rhizosphaerae</i> JJ-79^T. The whole genome of strain C159^T was 4.65 Mb, with a DNA G+C content of 38.65 mol%. The average nucleotide identity between strain C159^T and <i>P. rhizosphaerae</i> JJ-79^T was 75.31%, and the digital DNA-DNA hybridization value was 19.6%, supporting its designation as a novel species of the genus <i>Pseudoneobacillus</i>. The major respiratory quinone of strain C159^T was menaquinone 7; the major fatty acids were iso-C_14:0, iso-C_15:0 and anteiso-C_15:0; and the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain C159^T represents a novel species in the genus <i>Pseudoneobacillus</i>, for which the name <i>Pseudoneobacillus rhizolycopersici</i> sp. nov. is proposed. The type strain is C159^T (=GDMCC 1.5552^T=KCTC 43820^T). In addition, strain C159^T possesses the ability to produce indole-3-acetic acid (33.72 mg·l^-1) and to mineralize insoluble organic phosphorus. In a pot experiment, this strain demonstrated a plant growth-promoting effect of 22.68-44.49% and a disease suppression efficacy of 89.29% against bacterial wilt of tomato caused by <i>Ralstonia solanacearum</i>. The above results confirm that strain C159^T is a novel multifunctional rhizobacterium for the biocontrol of tomato bacterial wilt.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148792998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Leucogemmata humicola</i> gen. nov., sp. nov., an oligotrophic bacterium of the under-represented phylum <i>Gemmatimonadota</i> isolated from soil.","authors":"Rikuta Suzuki, Yumi Shimomura, Tomoki Iwashita, Xiang-Ying Meng, Kazuki Mori, Akiko Enomoto, Setsu Kato, Yutaka Nakashimada, Kosuke Tashiro, Hideyuki Tamaki, Yoshiteru Aoi","doi":"10.1099/ijsem.0.007204","DOIUrl":"10.1099/ijsem.0.007204","url":null,"abstract":"<p><p>A novel slow-growing bacterium, designated strain ag2_l2_5A^T, was isolated from pine forest soil at Kagamiyama, Hiroshima, Japan, by using a gel microdroplet cultivation approach. The strain was characterized as a Gram-negative, non-motile bacterium with a short rod-shaped morphology. The strain formed small, circular, smooth and umbonate colonies after prolonged incubation on agar plates. Cells accumulated intracellular polyphosphate and produced membrane vesicles during the exponential growth phase. The strain grew optimally at 20-30 °C and pH 6.0-7.5, utilizing various sugars, amino acids and organic acids as carbon sources. The genomic G+C content was 62.8%. The major fatty acids were iso-C_15 : 0, C_16 : 1 ω7c, iso-C_16 : 0, iso-C_17 : 0 and C_16 : 0. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain ag2_l2_5A^T belongs to the class <i>Gemmatimonadetes</i> within the phylum <i>Gemmatimonadota</i>. Its closest relatives were <i>Gemmatimonas phototrophica</i> AP64^T (91.3% gene sequence similarity), <i>Gemmatimonas aurantiaca</i> T-27^T and <i>Pseudogemmatithrix spongiicola</i> 318^T. Based on polyphasic characterization, the strain ag2_l2_5A^T represents a novel genus and species of the class <i>Gemmatimonadetes</i>, for which the name <i>Leucogemmata humicola</i> gen. nov., sp. nov. is proposed. The type strain is ag2_l2_5A^T (=NBRC 117234^T=DSM120521^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148360789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Marinomonas algae</i> sp. nov. and <i>Marinomonas phycocola</i> sp. nov., two novel bacteria isolated from marine algae.","authors":"Yea-Lin Moon, Kyoungdong You, Kyung Hyun Kim, Jin-Sook Park","doi":"10.1099/ijsem.0.007247","DOIUrl":"https://doi.org/10.1099/ijsem.0.007247","url":null,"abstract":"<p><p>Two novel bacterial strains, designated as 2405UD66-6^T and 2405UD68-3^T, were isolated from marine algae collected in the Republic of Korea. Both strains were Gram-stain-negative, strictly aerobic, motile, rod-shaped and oxidase-positive. Optimal growth occurred at 28 °C and pH 7.0, with NaCl concentrations of 3.0-4.0% (w/v) for strain 2405UD66-6^T and 2.0-3.0% (w/v) for strain 2405UD68-3^T. Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on draft genome sequences revealed that strains 2405UD66-6ᵀ and 2405UD68-3ᵀ belong to the genus <i>Marinomonas</i>. Phylogenomic analysis revealed that strain 2405UD66-6^T was most closely related to <i>Marinomonas colpomeniae</i> SM2066^T, while strain 2405UD68-3^T was most closely related to <i>Marinomonas phaeophyticola</i> 15G1-11^T. Average nucleotide identity and digital DNA-DNA hybridization values between strain 2405UD66-6^T and its closest relative were 77.4 and 20.6%, respectively, whereas those between strain 2405UD68-3^T and its closest relative were 75.8 and 19.7%, respectively. These values are well below the accepted species delineation thresholds, indicating that both strains represent novel species within the genus <i>Marinomonas</i>. The genomic G+C contents for strains 2405UD66-6^T and 2405UD68-3^T were 42.5 and 40.5 mol%, respectively. The major polar lipids of both strains were phosphatidylglycerol and phosphatidylethanolamine, and the sole respiratory quinone was ubiquinone-8. The predominant fatty acids (>10.0%) in both strains were C_16 : 0, summed feature 3 (C_16 : 1 <i> ω6</i>c and/or C_16 : 1 <i> ω7</i>c) and summed feature 8 (C_18 : 1 <i> ω7</i>c and/or C_18 : 1 <i> ω6</i>c). Based on a polyphasic taxonomic approach, the names <i>Marinomonas algae</i> sp. nov. and <i>Marinomonas phycocola</i> sp. nov. are proposed, respectively. The type strain is 2405UD66-6^T (KACC 24016^T=MCCC 1K09776^T) for <i>M. algae</i> and 2405UD68-3^T (KACC 24017^T=MCCC 1K09819^T) for <i>M. phycocola</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148470590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four novel <i>Pseudoalteromonas</i> species isolated from seawater: <i>Pseudoalteromonas mucoides</i> sp. nov., <i>Pseudoalteromonas aquimarina</i> sp. nov., <i>Pseudoalteromonas maris</i> sp. nov. and <i>Pseudoalteromonas thalassia</i> sp. nov.","authors":"Inhyup Kim, Sunho Park, Chunghwan Baek, Seunghui Kwak, Haejin Woo, Hyunji Lee, Subin Yook, Jisu Kim, Taegun Seo","doi":"10.1099/ijsem.0.007235","DOIUrl":"10.1099/ijsem.0.007235","url":null,"abstract":"<p><p>Four novel marine bacteria, ZZD1^T, SSM20^T, SSDWG2^T and SSMSWG5^T, were collected from seawater at Jajakdo and Minmeoru Beaches, Republic of Korea. The cells of these strains were Gram-stain-negative and rod-shaped. Strains ZZD1^T, SSM20^T, SSDWG2^T and SSMSWG5^T had the highest 16S rRNA gene sequence similarity to <i>Pseudoalteromonas carrageenovora</i> IAM 12662^T (16S rRNA gene sequence similarity, 99.1%), <i>Pseudoalteromonas spongiae</i> UST010723-006^T (99.2%), <i>Pseudoalteromonas caenipelagi</i> JBTF-M23^T (98.4%) and <i>Pseudoalteromonas shioyasakiensis</i> SE3^T (99.2%), respectively. Polyphasic and phylogenetic analyses confirmed that the four strains were closely related to the genus <i>Pseudoalteromonas</i>. The digital DNA-DNA hybridization values ranged from 18.9 to 22.2% for ZZD1^T, 19.1 to 26.0% for SSM20^T, 19.7 to 22.8% for SSDWG2^T and 19.7 to 34.5% for SSMSWG5^T. All values were thus below the 70% threshold, indicating distinct genomic differences between the strains. In addition, the average nucleotide identity and average amino acid identity of ZZD1^T, SSM20^T, SSDWG2^T and SSMSWG5^T with other species of the genus <i>Pseudoalteromonas</i> included in the phylogenetic analysis were 66.6-93.1% and 70.4-88.2%, respectively. Collectively, these results confirmed that the four strains represented new species within the genus <i>Pseudoalteromonas</i>. Based on these findings, the strains were classified as <i>Pseudoalteromonas mucoides</i> sp. nov. (type strain ZZD1^T=KACC 23717^T=TBRC 19020^T), <i>Pseudoalteromonas aquimarina</i> sp. nov. (type strain SSM20^T=KACC 23718^T=TBRC 19019^T), <i>Pseudoalteromonas maris</i> sp. nov. (type strain SSDWG2^T=KACC 23719^T=TBRC 19021^T) and <i>Pseudoalteromonas thalassia</i> sp. nov. (type strain SSMSWG5^T=KACC 23734^T=TBRC 19022^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}