International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Janibacter alittae</i> sp. nov., an actinobacterium isolated from the gut of marine sandworm (<i>Alitta virens</i>).","authors":"Bora Kim, Min Ok Jun, Ah-In Yang, Sung-Hong Joe, Hae-In Joe, Hojun Sung, Ki Hyun Kim, Hyun Jung Kim, Na-Ri Shin","doi":"10.1099/ijsem.0.006557","DOIUrl":"https://doi.org/10.1099/ijsem.0.006557","url":null,"abstract":"<p><p>A novel Gram-stain-positive, facultatively anaerobic, non-motile, catalase-positive, oxidase-negative and ovoid cocci, designated as A1S7^T, was isolated from the gut of a marine sandworm (<i>Alitta virens</i>). Strain A1S7^T exhibited optimal growth at temperatures of 20-30 ℃, pH 6-8 and in the presence of 2-4% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain A1S7^T belonged to the genus <i>Janibacter</i>, exhibiting a similarity of 99.0% to <i>Janibacter cremeus</i> KCTC 49873^T, followed by <i>Janibacter anophelis</i> KCTC 19282^T (98.8%), <i>Janibacter hoylei</i> KCTC 49872^T (98.4%), <i>Janibacter limosus</i> KACC 20518^T (98.2%) and <i>Janibacter corallicola</i> KACC 21120^T (97.2%). The complete genome sequence of strain A1S7^T revealed a genome size of 3360920 bp with a genomic G+C content of 70.1 mol%. The orthologous average nucleotide identity and the digital DNA-DNA hybridization values between strain A1S7^T and <i>Janibacter cremeus</i> KCTC 49873^T were determined to be 89.5 and 37.2%, respectively. The major respiratory quinone was MK-8(H₄). The predominant fatty acids (>10%) included <i>iso</i>-C_16:0, C_17:1 <i> ω</i>8<i>c</i>, C_18:1 <i> ω</i>9<i>c</i> and C_17:0. Polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and three unknown polar lipids. The cell-wall peptidoglycan type was A1<i>γ</i>. The major whole-cell sugars were ribose, mannose and glucose. Based on phenotypic, phylogenetic, genotypic and chemotaxonomic properties, strain A1S7^T represents a novel species in the genus <i>Janibacter</i>, for which the name <i>Janibacter alittae</i> sp. nov. is proposed. The type strain is A1S7^T (=KCTC 49714^T = JCM 36706^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Yamadazyma thunbergiae</i> sp. nov., a novel yeast species associated with Bengal clock vines and soil in Okinawa, Japan.","authors":"Taisuke Seike, Hiroki Takekata, Natsue Sakata, Chikara Furusawa, Fumio Matsuda","doi":"10.1099/ijsem.0.006537","DOIUrl":"10.1099/ijsem.0.006537","url":null,"abstract":"<p><p>Two strains, designated JCM 36746^T and JCM 36749, were isolated from Bengal clock vine (<i>Thunbergia grandiflora</i>) and soil, respectively, in Okinawa, Japan. Analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit rRNA gene sequences revealed identical sequences in both strains, indicating that they belong to the same species. Sequence analysis and physiological characterization identified these strains as representing a novel yeast species in the genus <i>Yamadazyma</i>. The sequence similarities of the concatenated ITS regions and D1/D2 domains indicated that JCM 36746^T and JCM 36749 formed a well-supported distinct from closely related species belonging to the <i>Yamadazyma</i> clade, including <i>Candida dendronema</i>, <i>C. diddensiae</i>, <i>C. germanica</i>, <i>C. kanchanaburiensis</i>, <i>C. naeodendra</i>, <i>C. vaughaniae</i>, <i>Y. akitaensis</i>, <i>Y. koratensis</i>, <i>Y. nakazawae</i>, <i>Y. philogaea</i>, <i>Y. phyllophila</i>, <i>Y. siamensis</i>, <i>Y. ubonensis</i>, and three undescribed species, comprising <i>Candida</i> aff. <i>naeodendra</i>/<i>diddensiae</i> Y151, <i>Candida</i> sp. GE19S08, and <i>Yamadazyma</i> sp. strain NYNU 22830. The sequences of the D1/D2 domains and ITS regions differed in nucleotide substitutions by 1.51% and 2.57% or greater, respectively, from those of the previously described and undescribed related species. In addition, the physiological characteristics of the novel species were distinct from those of the closely related described species. On the basis of these findings, we propose the name <i>Yamadazyma thunbergiae</i> sp. nov. to classify this species within the genus <i>Yamadazyma</i>. The holotype used is JCM 36746^T (ex-type strains CBS 18614 and NBRC 116657). The MycoBank accession number is MB 853823.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Amycolatopsis melonis</i> sp. nov., a novel protease-producing and cellulose-degrading actinobacterium isolated from soil.","authors":"Yan Xu, Xue Zhao, Jiaxin Jin, Ran Zhang, Changjian Zhou, Zishan Wang, Siqi Yao, Xiangjing Wang, Wensheng Xiang, Jia Song","doi":"10.1099/ijsem.0.006559","DOIUrl":"https://doi.org/10.1099/ijsem.0.006559","url":null,"abstract":"<p><p>A novel protease-producing and cellulose-degrading actinobacterium, designated strain NEAU-NG30^T, was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30^T was closely related to Amycolatopsis bullii DSM 45802^T (98.7%) and Amycolatopsis vancoresmycina DSM 44592^T (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained <i>meso</i>-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-9(H₄). The major fatty acids were iso-C_15:0 and iso-C_16:0. Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA-DNA hybridization between strain NEAU-NG30^T and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30^T is considered to be a novel species of the genus <i>Amycolatopsis</i>, for which the name <i>Amycolatopsis melonis</i> sp. nov. is proposed, with NEAU-NG30^T (=MCCC 1K08677^T=JCM 35654^T) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Millerago</i> gen. nov. based on taxogenomic analysis, with two new species, <i>Millerago phaffii</i> f.a., sp. nov. and <i>Millerago galiae</i> f.a., sp. nov.","authors":"Angela M García-Acero, Thiago M Batista, Gisele F L Souza, Ana Raquel O Santos, Daniela L Souza, Glória R Franco, Mario E Velásquez-Lozano, Daichi Yamamoto, Wataru Toki, Marc-André Lachance, Carlos A Rosa","doi":"10.1099/ijsem.0.006565","DOIUrl":"https://doi.org/10.1099/ijsem.0.006565","url":null,"abstract":"<p><p>Four yeast isolates obtained from tree bark and fermenting sap of <i>Quercus</i> spp. and insects in Colombia and Japan were phylogenetically related to <i>Candida galis</i> based on analyses of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene. The novel species differs from <i>C. galis</i> by 20 nt substitutions and 5 indels in the D1/D2 sequences. A phylogenomic analysis suggested that these species are related to <i>Candida ficus</i>, the genus <i>Phaffomyces</i> and a small clade containing <i>Barnettozyma botsteinii</i>, <i>Barnettozyma siamensis</i> and <i>Candida montana</i>. Our genomic analyses suggest that the novel species and <i>C. galis</i> should be separated in a novel yeast genus. We propose the genus <i>Millerago</i> gen. nov. to accommodate these species and the species <i>Millerago phaffii</i> f.a., sp. nov. (CBS 18021^T; MycoBank MB856172) to accommodate the Colombian and Japanese isolates. The Colombian isolate of <i>M. phaffii</i> differs from the Japanese isolates by three nt substitutions and one indel and two substitutions and one indel in the ITS and D1/D2 sequences, respectively, showing that they were conspecific. We also propose the new species <i>Millerago galiae</i> sp. nov. to validate this species according to the rules of the International Code of Nomenclature for algae, fungi and plants.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Paracoccus actinidiae</i> sp.nov., a novel bacterium isolated from kiwi tree rhizosphere soil.","authors":"Chenqi Xu, Han Peng, He Li, Xinran Xie, Siwei Chen, Junhao Dai, Hong Ren","doi":"10.1099/ijsem.0.006529","DOIUrl":"https://doi.org/10.1099/ijsem.0.006529","url":null,"abstract":"<p><p>Strain M09^T was isolated from the rhizoshere of kiwi fruit trees from an orchard located in Fangshan, Beijing, PR China (39° 49' 25.1″ N, 116° 4' 44.5″ E,). It is a short rod-shaped, Gram-stain-negative, facultatively anaerobic bacterium that tests positive for both oxidase and catalase. The strain exhibited growth within the temperature range of 15-45 °C (optimal growth at 30 °C) and the pH range of 4.0-10.0 (optimal growth at pH 7.0) and without NaCl. It also grew in a sodium chloride-free nutrient agar (NA) medium. The results of phylogenetic analysis of the 16S rRNA gene sequences indicated that M09^T represents a member of the genus <i>Paracoccus</i> and shares high similarity with <i>Paracoccus everestensis</i> S8-55^T (98.46%) and <i>Paracoccus aerius</i> 011410^T (97.58%). The average nucleotide identity values between M09^T and <i>P. everestensis</i> S8-55^T, <i>P. aerius</i> 011410^T, <i>Paracoccus marinaquae</i> X HP0099^T and <i>Paracoccus fontiphilus</i> MVW-1^T were 95.56, 84.51, 79.83 and 83.68%, respectively. The digital DNA-DNA hybridisation values between between M09^T and <i>P. everestensis</i> S8-55^T, <i>P. aerius</i> 011410^T, <i>P. marinaquae</i> X HP0099^T and <i>P. fontiphilus</i> MVW-1^T were 56.40, 29.30, 21.60 and 28.60%, respectively. The major fatty acids identified were C_10 : 0 3-OH (51.8%) and C_18 : 1ω7<i>c</i> (35.5%). The major respiratory quinone was Q-10, with Q-8 present as a minor component. Polar lipids were mainly comprised of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE). Genome sequencing revealed that the strain has a DNA G+C content of 64.31 mol%. On the basis of this comprehensive taxonomic characterisation data, M09^T represents a novel species within the genus <i>Paracoccus</i> and has been named <i>Paracoccus actinidiae</i> sp. nov. The type strain is designated as M09^T (=GDMCC 1.4157^T=KCTC 8143^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Leuconostoc koreense</i> sp. nov., isolated from kimchi, a fermented vegetable.","authors":"Min Ji Lee, Jeong Ui Yun, Sohee Nam, Mi-Ja Jung, Yeon Bee Kim, Yujin Kim, Jisu Lee, Dae Gyu Choi, Dae Seung Lee, Seong Woon Roh, Tae Woong Whon, Che Ok Jeon, Se Hee Lee","doi":"10.1099/ijsem.0.006533","DOIUrl":"https://doi.org/10.1099/ijsem.0.006533","url":null,"abstract":"<p><p>The novel strain CBA3628^T was isolated from kimchi, a Korean fermented vegetable. CBA3628^T is a cocci-shaped, Gram-stain-positive, catalase- and oxidase-negative and facultatively anaerobic bacterium. The results of phylogenetic analysis based on 16S rRNA gene sequencing indicated that CBA3628^T represented a member of the genus <i>Leuconostoc</i> of the family <i>Leuconostocaceae</i>. CBA3628^T has a circular chromosomal genome and three plasmids of 1 864 558 bp (37% DNA G+C content), containing 1,887 genes, 1,762 predicted protein-coding genes, 4 complete rRNA loci and 70 tRNA genes. The cells were non-haemolytic, non-motile and non-spore forming. The optimal growth of CBA3628^T occurred at 30 °C, pH 6.0 and with 0-2% (w/v) NaCl. The major polar lipids of CBA3628^T were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids (>10%) of CBA3628^T were C_{16  :  0}, C_{20  :  0} and C_19 : 0 cyclo ω8<i>c</i>. CBA3628^T contained A3α-type peptidoglycans. CBA3628^T was most closely related to <i>Leuconostoc mesenteroides</i> subsp. <i>mesenteroides</i> ATCC 8293^T, <i>L. mesenteroides</i> subsp. <i>dextranicum</i> DSM 20484^T and <i>L. suionicum</i> DSM 20241^T with 99.52% 16S rRNA gene sequence similarity. However, the average nucleotide identities of 91.9%, 91.7% and 91.1% and the digital DNA-DNA hybridisation values of 45.6%, 45.4% and 45.4% indicated that the novel isolate represented a distinct species. Phylogenetic analyses of both the 16S rRNA gene and genome sequences revealed that CBA3628^T formed a distinct phylogenetic lineage within the genus <i>Leuconostoc</i> and was most closely related to <i>Leuconostoc litchii</i> MB7^T. The ANI and dDDH values between CBA3628^T and <i>L. litchii</i> MB7^T were 84.9 and 22.8%, respectively. Functional genes belonging to COG categories E, J and K were enriched in the genome of CBA3628^T (>7.9%). On the basis of its physiological, chemotaxonomic, phylogenetic and genomic properties, strain CBA3628^T represents a novel species from the genus <i>Leuconostoc</i>, for which we propose the name <i>Leuconostoc koreense</i> sp. nov., with the type strain CBA3628^T (= KACC 23049^T = DSM 116836^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Yoonia algicola</i> sp. nov., <i>Yoonia rhodophyticola</i> sp. nov. and <i>Yoonia phaeophyticola</i> sp. nov., isolated from marine algae.","authors":"Dong Min Han, Ji Hoon Jeon, Myeong Seo Jin, Dae Gyu Choi, Jeong Min Kim, Hülya Bayburt, Byeong Jun Choi, Che Ok Jeon","doi":"10.1099/ijsem.0.006545","DOIUrl":"https://doi.org/10.1099/ijsem.0.006545","url":null,"abstract":"<p><p>Three Gram-stain-negative, strictly aerobic, non-motile, oxidase- and catalase-positive, short-rod-shaped bacteria, designated as strains G8-12^T, SS1-5^T and BS5-3^T, were isolated from marine algae in South Korea. Strain G8-12^T exhibited optimal growth at 20-25 °C, pH 8.0 and 2.0-2.5% (w/v) NaCl, while strains SS1-5^T and BS5-3^T grew optimally at 25 °C, pH 7.0 and 1.5% NaCl. All strains contained ubiquinone-10 as the sole respiratory quinone, with phosphatidylglycerol and phosphatidylcholine as major polar lipids, and C_18 : 1 <i> ω</i>7<i>c</i> and C_16 : 0 as major fatty acids (>5 %); C_18 : 1 <i> ω</i>7<i>c</i> 11-methyl and C_18 : 1 2-OH were additionally identified as major fatty acids in strain SS1-5^T. The genomic DNA G+C contents were 57.0, 58.3 and 56.4% for strains G8-12^T, SS1-5^T and BS5-3^T, respectively. Strains G8-12^T, SS1-5^T and BS5-3^T exhibited less than 74.8% average nucleotide identity (ANI) and 19.7% digital DNA-DNA hybridization (dDDH) values with each other, indicating that they represent different species. Phylogenetic analyses based on both 16S rRNA gene and genome sequences revealed that strains G8-12^T, SS1-5^T and BS5-3^T form distinct phylogenetic lineages within the genus <i>Yoonia</i>. Relative to other closely related <i>Yoonia</i> species, these strains exhibited ANI and dDDH values below 83.5 and 26.9%, respectively, suggesting that they constitute novel species within the genus <i>Yoonia</i>. Based on their phenotypic, chemotaxonomic and phylogenetic characteristics, strains G8-12^T, SS1-5^T and BS5-3^T represent three novel species of the genus <i>Yoonia</i>, for which the names <i>Yoonia algicola</i> sp. nov. (G8-12^T=KACC 22753^T=JCM 35790^T), <i>Yoonia rhodophyticola</i> sp. nov. (SS1-5^T=KACC 22649^T=JCM 35753^T) and <i>Yoonia phaeophyticola</i> sp. nov. (BS5-3^T=KACC 22648^T=JCM 35751^T) are proposed, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11482539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Flavobacterium capsici</i> sp. nov., isolated from the rhizospheric soils of bell pepper (<i>Capsicum annuum</i> L.).","authors":"Dong Hyun Cho, Yuxin Peng, Zalfa Humaira, Yu Lim Park, Hyun Jung Kim, Rae-Dong Jeong, Cha Young Kim, Jiyoung Lee","doi":"10.1099/ijsem.0.006554","DOIUrl":"https://doi.org/10.1099/ijsem.0.006554","url":null,"abstract":"<p><p>Two separate bacterial strains, PMTSA4^T and PMR2A8, were isolated from the rhizospheric soils of bell pepper plants grown in a plant nursery. These strains are Gram-negative, non-motile and rod-shaped and grow in aerobic conditions. They exhibit a positive reaction for catalase activity but negative results for oxidase activity. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the strains PMTSA4^T and PMR2A8 are closely related to <i>Flavobacterium piscinae</i> ICH-30^T (95.6%, respectively), <i>Flavobacterium ahnfeltiae</i> 10Alg 130^T (95.5%) and <i>Flavobacterium maris</i> KMM 9535^T (95.3%), aligning them within the genus <i>Flavobacterium</i>. Digital DNA-DNA hybridization (dDDH) values and average nucleotide identities (ANIs) of the whole-genome sequences for the two strains and related <i>Flavobacterium</i> species were significantly below the established thresholds for prokaryotic species delineation (<70% for dDDH and <95% for ANI). The observed values were as follows: <i>Flavobacterium aquatile</i> LMG 4008^T (dDDH: 19.8% and ANI: 75.5%), <i>F. piscinae</i> ICH-30^T (dDDH: 18.6% and ANI: 73.3%) and <i>F. stagni</i> WWJ 16^T (dDDH: 18.5% and ANI: 72.0%). The strains have genome sizes of 3 068 185 bp and 3 068 330 bp, with a G+C content of 32.5 mol%. In phenotypic characterization, the new strains grew at 10-35 °C and tolerated up to 4% NaCl at pH 5-9 (optimum pH 8). The predominant cellular fatty acids were observed to be iso-C_15:0, iso-C_17:0 3-OH and iso-C_15:0 3-OH. Menaquinone-6 was the predominant quinone. Considering the results from phenotypic, chemotaxonomic, phylogenetic and genomic analyses, it is proposed that the strains PMTSA4^T and PMR2A8 represent a novel species within the genus <i>Flavobacterium</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Campylobacter californiensis</i> sp. nov., isolated from cattle and feral swine.","authors":"William G Miller, Mary H Chapman, Tina G Williams, Delilah F Wood, James L Bono, David J Kelly","doi":"10.1099/ijsem.0.006524","DOIUrl":"10.1099/ijsem.0.006524","url":null,"abstract":"<p><p>Nine <i>Campylobacter</i> strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 <i>Campylobacter</i>-associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to <i>Campylobacter concisus</i> and <i>Campylobacter mucosalis</i>. Additional <i>atpA</i> typing indicated that the nine strains composed a discrete novel clade related to <i>C. concisus</i> and <i>C. mucosalis</i>. A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other <i>Campylobacter</i> taxa, indicating that these strains represent a novel <i>Campylobacter</i> species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative <i>Campylobacter</i> species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within <i>Campylobacter</i>, for which the name <i>Campylobacter californiensis</i> sp. nov. (type strain RM6914^T=LMG 32304^T=CCUG 75329^T) is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Halostreptopolyspora alba</i> gen. nov., sp. nov., a halophilic actinobacterium isolated from saline soil of Xinjiang, Northwest of China.","authors":"Rui Li, Xiao-Jing Tang, Yu-Zhou Feng, Yang Ji, Yun Wang, Cui-Ping Miao, Mei Tang, Ke-Ke Wang, Wei-Xun Chunyu, Li-Xing Zhao, Shu-Kun Tang","doi":"10.1099/ijsem.0.006484","DOIUrl":"10.1099/ijsem.0.006484","url":null,"abstract":"<p><p>A Gram-stain-positive, aerobic, moderate halophilic actinobacterium, designated strain YIM 96095^T, was isolated from a saline soil sample collected from Aiding Lake, Xinjiang, North-western China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate belonged to the family <i>Nocardiopsidaceae</i>, formed a distinct subclade, and was most closely related to <i>Lipingzhangella halophila</i> DSM 102030^T and <i>Allosalinactinospora lopnorensis</i> DSM 45697^T with sequence identity values of 95.8 and 95.1%, respectively. Optimal growth occurred at 37 °C, pH 7.0-8.0 and with 5-16% (w/v) NaCl, with well-developed, non-fragmented substrate mycelia and single-, double-, or triple-wrinkled spore(s) on the mature aerial hyphae. The chemical analysis presented <i>meso</i>-diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan, and glucose, galactose and rhamnose as the major whole-cell sugars, and iso-C_15 : 0 and anteiso-C_15 : 0 as the major fatty acids. The phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids and unidentified glycolipid. The menaquinones were MK-10(H₈), MK-10(H₆) and MK-9(H₁₀). Its G+C content was 69.7 mol% in the determined genome sequence. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, a novel genus and species named <i>Halostreptopolyspora alba</i> gen. nov., sp. nov. is proposed for isolate YIM 96095^T (=KCTC 49266^T=CGMCC 4.7636^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}