International journal of systematic and evolutionary microbiology最新文献

{"title":"Reclassification of <i>Algoriphagus aquimaris</i> Kim <i>et al</i>. 2020 as a later heterotypic synonym of <i>Algoriphagus formosus</i> Ye <i>et al</i>. 2020 using a polyphasic approach.","authors":"Jiaxing Liu, Mengyin Fu, Ping Mo, Bailu Tang, Fengying Liu","doi":"10.1099/ijsem.0.006887","DOIUrl":"10.1099/ijsem.0.006887","url":null,"abstract":"<p><p>16S rRNA gene sequence analysis indicated that <i>Algoriphagus aquimaris</i> F21^T shared the highest sequence similarity of 100.00% to <i>Algoriphagus formosus</i> XAY3209^T. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that <i>A. aquimaris</i> F21^T formed a cluster with <i>A. formosus</i> XAY3209^T, suggesting that there was a close genetic relationship between them. However, the average nucleotide identity (ANIb/m) and digital DNA-DNA hybridization values between <i>A. aquimaris</i> F21^T and <i>A. formosus</i> XAY3209^T were 97.48%/97.73% and 79.50%, respectively, greater than the 95-96.00% and 70.00% cut-off points recommended for delineating bacterial species, which suggested that these two strains belonged to the same species. In addition, the comprehensive comparison of phenotypic and chemotaxonomic characteristics further verified this conclusion. Based on all these data above and rule 42 of the International Code of Nomenclature of Prokaryotes, we propose that <i>A. aquimaris</i> Kim <i>et al</i>. 2020 is a later heterotypic synonym of <i>A. formosus</i> Ye <i>et al</i>. 2020.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Qipengyuania marincola</i> sp. nov., <i>Qipengyuania flavescens</i> sp. nov. and <i>Pseudoblastomonas aquimaris</i> sp. nov., three novel members of the family <i>Erythrobacteraceae</i> isolated from seawater.","authors":"Tian-Pu Li, Dao-Feng Zhang, Zi-Yue Fu, Meng-Han Huang","doi":"10.1099/ijsem.0.006924","DOIUrl":"https://doi.org/10.1099/ijsem.0.006924","url":null,"abstract":"<p><p>Three Gram-stain-negative, aerobic, rod-shaped, non-motile and orange-pigmented bacteria, designated as XHP0207^T, XHP0211^T and WL0013^T, were isolated from surface seawater collected from Nantong City, the Yellow Sea of China. Cells were positive for catalase and oxidase. These strains were all optimally grown at 28 ℃, pH 7.0 and 3% (w/v) NaCl and possessed Q-10 as the respiratory quinone. The 16S rRNA gene sequence analysis indicated that strains XHP0207^T, XHP0211^T and WL0013^T showed the highest sequence similarity to <i>Qipengyuania profundimaris</i> G39^T (99.0%), <i>Q. profundimaris</i> G39^T (100%) and <i>Alteriqipengyuania flavescens </i>SCSIO 75105^T (100%), respectively. However, a phylogenetic tree based on the 288 orthologous clusters indicated that both XHP0207^T and XHP0211^T were closely related to <i>Qipengyuania proteolytica</i> 6B39^T, and WL0013^T was closely related to <i>Alterqipengyuania flavescens</i> SCSIO 75105^T. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values among strains XHP0207^T, XHP0211^T and WL0013^T and closely related type strains were all below the species delineation cutoffs of 95%, 95% and 70%, respectively, though the values exceeded between strain XHP0207^T and a non-type strain. The genomic DNA G+C contents of strains XHP0207^T, XHP0211^T and WL0013^T were 63.0%, 63.0% and 65.5%, respectively. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, strains XHP0207^T and XHP0211^T represent two novel <i>Qipengyuania</i> species, and we thus propose the names <i>Qipengyuania marincola</i> sp. nov. (type strain, XHP0207^T=MCCC 1K07527^T=GDMCC 1.3090^T=JCM 35571^T) and <i>Qipengyuania flavescens</i> sp. nov. (type strain, XHP0211^T=MCCC 1K07528^T=GDMCC 1.3091^T=JCM 35572^T). Strain WL0013^T represents a novel <i>Pseudoblastomonas</i> species, for which the name <i>Pseudoblastomonas aquimaris</i> sp. nov. (type strain, WL0013^T=JCM 34654^T=GDMCC1.2439^T) is proposed. In addition, <i>Alterqipengyuania flavescens</i> is reclassified as <i>Pseudoblastomonas flavescens</i> comb. nov. (type strain, KCTC 92502^T=MCCC 1K07993^T=SCSIO 75105^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging the subgenus category to address monophyletic genus over-splitting: illustration with recently proposed <i>Mycobacteriales</i> genera.","authors":"Jorge Val-Calvo, Mariela Scortti, Markus Göker, José A Vázquez-Boland","doi":"10.1099/ijsem.0.006917","DOIUrl":"10.1099/ijsem.0.006917","url":null,"abstract":"<p><p>Three related circumstances are affecting the stability of prokaryotic taxonomy and nomenclature, with significant implications in the field of pathogenic micro-organisms: (i) the subjective application of genomics-based demarcation criteria to subdivide monophyletic genera, creating an increasing number of new genera; (ii) databases' preference for the latest validly published names; and (iii) the practical irreversibility of new names in databases, even when later taxonomic opinion supports reverting to previous classifications. Due to understandable end-user reluctance to accept name changes affecting well-known pathogens, parallel nomenclatures coexist, causing confusion. To address this issue, we propose using the subgenus category to mitigate the disruptive impact of genus name changes in databases. Specifically, we suggest lowering to subgenus rank those new genera arising from monophyletic genera splits that have limited practical utility and may contribute to taxonomic instability. Under the International Code of Nomenclature of Prokaryotes, the species' generic name would revert to its previous synonym, optionally followed in parentheses by the validly published subgenus name (corresponding to the latest genus synonym used in databases). Because the subgenus is an optional taxonomic category, it may be omitted; however, its use may facilitate the mapping of synonyms in databases and literature. We illustrate this strategy through its application to recent genus splits in the <i>Mycobacteriales</i>, specifically the genus <i>Prescottella</i> nested within the rhodococcal radiation, and the several genera into which <i>Mycobacterium</i> was subdivided.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and molecular basis of cell-cell recognition in <i>Euplotes songi</i> sp. nov., a phylogenetically early-emerging new species of <i>Euplotes</i> (Ciliophora, Spirotrichea).","authors":"Claudio Alimenti, Yaohan Jiang, Graziano Di Giuseppe, Bill Pedrini, Pierangelo Luporini, Adriana Vallesi","doi":"10.1099/ijsem.0.006907","DOIUrl":"https://doi.org/10.1099/ijsem.0.006907","url":null,"abstract":"<p><p><i>Euplotes songi</i> sp. nov. is described as a new morphospecies of <i>Euplotes</i>, isolated from shallow interstitial waters off the southern Italian coast of the Adriatic Sea. Its small (49×33 µm) body has a slightly asymmetric elliptical shape, contains a relatively large hook-shaped macronucleus consistently associated with a single micronucleus and harbours <i>γ</i>-proteobacteria as endosymbionts. Formation of resting stages was not observed, either as a result of culture ageing or in response to conditions commonly inducing encystation. The dorsal surface features a silver-line system ('dargyrome') of the double type, with two longitudinal inter-kinety rows of argentophilic polygons that are markedly unequal in width. Four prominent longitudinal ridges, each bearing one kinety of 9-12 dikinetids, run along the dorsal body side, while two shorter kineties extend along the lateral cell margins. The ventral surface bears 10 fronto-ventral, five transverse, two stiff and elongated caudal cirri and a single short, highly motile marginal cirrus. The buccal field extends nearly four-fifths of the body length and is bordered by 28-31 adoral membranelles. In the <i>Euplotes</i> SSU rRNA gene-based phylogenetic tree, <i>E. songi</i> clusters with <i>Euplotes huizhouensis</i>, <i>Euplotes petzi</i> and <i>Euplotes sinicus</i>, forming the earliest emerging clade. Consistent with other <i>Euplotes</i> species, <i>E. songi</i> cells grow and mate under the control of a family of waterborne, cell-type-specific protein pheromones. Native pheromones, purified from genetically distinct cultures, are active at micromolar concentrations and consist of short 33-aa sequences, which include eight cysteine residues and fold into a bundle of three antiparallel helical segments. This bundle likely represents an evolutionary forerunner of the more complex helical bundles characteristic of pheromone families synthesized by phylogenetically derived <i>Euplotes</i> species. A simple organization is similarly exhibited by the macronuclear pheromone-coding genes, having full-length sequences of only 728 or 732 bp and an intron-free ORF.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Notification that new names of prokaryotes, new combinations and new taxonomic opinions have appeared in volume 75, part 5 of the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006824","DOIUrl":"10.1099/ijsem.0.006824","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 8","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Nocardioides lacus</i> sp. nov., a novel member of the <i>Actinomycetota</i> isolated from a freshwater pond.","authors":"Young Ho Nam, Ji Young Jung, Hye Kyeong Kang, Byung-Gon Ryu, Ye-Ji Hwang, Tae Jin Kim, Mi-Hwa Lee, Hyun Mi Jin, Kook-Il Han","doi":"10.1099/ijsem.0.006929","DOIUrl":"10.1099/ijsem.0.006929","url":null,"abstract":"<p><p>A Gram-positive, aerobic, short rod-shaped, white-coloured bacterium, designated strain BP30^T, was isolated from a freshwater pond. Growth occurred at 15-35 °C (optimum, 30 °C) and pH 5.5-8.5 (optimum, pH 7) in the presence of 0-0.5 % NaCl (optimum, 0 %). 16S rRNA gene phylogenetic analysis showed that this strain was most closely related to <i>Nocardioides marinus</i> CL-DD14ᵀ (97.07%), <i>Nocardioides terrae</i> VA15ᵀ (97.03%), <i>Nocardioides opuntiae</i> OS1-21ᵀ (96.96%), <i>Nocardioides panacihumi</i> Gsoil 616ᵀ (96.94%) and <i>Nocardioides nematodiphilus</i> R-N-C8ᵀ (96.87%) but formed a distinct lineage within the genus <i>Nocardioides</i>. Phylogenetic and phylogenomic analyses further revealed that this strain clustered with <i>N. nematodiphilus</i> R-N-C8ᵀ and <i>Nocardioides baekrokdamisoli</i> B2-12ᵀ (96.04%) in the tree. The predominant respiratory quinone was menaquinone-8 (H₄), and the major fatty acids were iso-C_16 : 0, C_18 : 0 and 10-methyl C_18 : 0 (tuberculostearic acid). The polar lipids in strain BP30^T consisted primarily of phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 70.9 mol%. Phylogenetic, average nt identity, digital DNA-DNA hybridization, phylogenomic, physiological and biochemical data indicated that the novel strain was distinct from other members of the genus <i>Nocardioides</i>. Notably, genome annotation of strain BP30^T revealed broad metabolic versatility, including complete pathways for central carbohydrate and aa metabolism and the biosynthesis of key cofactors and vitamins. The presence of genes for the degradation of aromatic hydrocarbons suggests a potential role in the bioremediation of polluted environments. Based on the genotypic, phenotypic and chemotaxonomic features, strain BP30^T (=KACC 22205^T=JCM 34558^T) is proposed as a novel species of the genus <i>Nocardioides</i>, named <i>Nocardioides lacus</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of two novel <i>Micromonospora</i> species: <i>Micromonospora psammae</i> sp. nov. from desert soil and <i>Micromonospora lacuserhaii</i> sp. nov. from lake sediment.","authors":"Yang Deng, Cong-Jian Li, Hua-Hong Chen, Li-Yan Yu, Man Cai, Yu-Qin Zhang","doi":"10.1099/ijsem.0.006922","DOIUrl":"https://doi.org/10.1099/ijsem.0.006922","url":null,"abstract":"<p><p>Three strains - CPCC 205556^T and CPCC 205543 isolated from gravel soil in China's Gurbantunggut Desert and CPCC 205547^T obtained from Erhai Lake in Yunnan - were characterized as Gram-stain-positive, orange to dark brown-pigmented actinobacteria. These isolates were mesophilic, exhibiting optimal growth at pH 7.0-8.0. Phylogenetic analyses based on 16S rRNA gene sequences placed these isolates within the genus <i>Micromonospora</i>, with high similarity (>99%) to <i>Micromonospora solifontis</i> NBRC 113441^T and <i>Micromonospora veneta</i> DSM 109713^T. Chemotaxonomic analyses (fatty acids, polar lipids and peptidoglycan) and the genomic G+C content further supported their classification within <i>Micromonospora</i>. However, whole-genome comparisons revealed distinct taxonomic relationships: strains CPCC 205556^T and CPCC 205543 shared 85.6-85.7% average nucleotide identity (ANI) and 33.5% digital DNA-DNA hybridization (dDDH) with <i>Micromonospora auratinigra</i> DSM 44815^T, while CPCC 205547^T shared 85.7% of ANI and 32.0% of dDDH with <i>Micromonospora coxensis</i> JCM 13248^T. Notably, CPCC 205556^T and CPCC 205543 exhibited near-identical genomic relatedness (ANI=99.3%, dDDH=93.3%), confirming their status as a single novel species. Based on these findings, two novel species were proposed: <i>Micromonospora psammae</i> sp. nov. (type strain CPCC 205556^T=MS1047^T=KCTC 59310^T), representing the desert isolates, and <i>Micromonospora lacuserhaii</i> sp. nov. (type strain CPCC 205547^T=EH501^T=KCTC 59309^T) from the lake habitat. Genomic analysis highlighted their biosynthetic potential for specialized metabolites, consistent with the genus's recognized metabolic diversity.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145175326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation list no. 225: valid publication of new names and new combinations effectively published outside the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006863","DOIUrl":"10.1099/ijsem.0.006863","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse biofilm-forming <i>Sphingomonadaceae</i> represent twelve novel species isolated from glaciers on the Tibetan Plateau.","authors":"Dou Han, Yu-Hua Xin, Qing Liu","doi":"10.1099/ijsem.0.006913","DOIUrl":"10.1099/ijsem.0.006913","url":null,"abstract":"<p><p>The family <i>Sphingomonadaceae</i>, encompassing the genus <i>Sphingomonas</i> and related taxa, comprises diverse Gram-negative, aerobic, rod-shaped bacteria found in varied habitats, including air, soil, water and glaciers. Recent genomic-based taxonomic revisions have reclassified some <i>Sphingomonas</i> species into new genera, such as <i>Parasphingomonas</i> and <i>Alteristakelama</i>, due to polyphyletic relationships within the family <i>Sphingomonadaceae</i>. Certain <i>Sphingomonadaceae</i> species are known for forming biofilms or functioning as aerobic anoxygenic phototrophic bacteria, traits that enhance resilience in extreme environments like the cryosphere. In this study, we isolated 12 novel strains from Tibetan Plateau glaciers, revealing significant phenotypic and genotypic diversity. Based on phylogenomic analyses, six strains were classified within <i>Sphingomonas</i>, five within <i>Parasphingomonas</i> and one within <i>Alteristakelama</i>. These strains exhibit broad pH (4-11), salt tolerance (0-3.0%) and temperature adaptability (0-37 °C), alongside varied metabolic capabilities, including diverse carbon source utilization and enzyme activities. Eleven strains exhibit biofilm formation, and some possess genes for carotenoid biosynthesis and photosynthesis. The 16S rRNA gene sequence similarities with their closest relatives ranged from 97.6% to 99.9%, while the average nt identity and digital DNA-DNA hybridization values between these strains and known species with validly published names were below 89.80% and 36.60%, respectively. Polyphasic analyses, encompassing phylogenetic, phenotypic and genotypic analyses, confirm that these strains represent 12 novel species within the family <i>Sphingomonadaceae</i>. We propose the following names: <i>Alteristakelama amylovorans</i> sp. nov., <i>Sphingomonas sorbitolis</i> sp. nov., <i>Sphingomonas fucosidasi</i> sp. nov., <i>Sphingomonas sandaracina</i> sp. nov., <i>Sphingomonas rhamnosi</i> sp. nov., <i>Sphingomonas arabinosi</i> sp. nov., <i>Sphingomonas flavida</i> sp. nov., <i>Parasphingomonas frigoris</i> sp. nov., <i>Parasphingomonas halimpatiens</i> sp. nov., <i>Parasphingomonas zepuensis</i> sp. nov., <i>Parasphingomonas caseinilytica</i> sp. nov. and <i>Parasphingomonas puruogangriensis</i> sp. nov. This study enhances our understanding of <i>Sphingomonadales</i> diversity and its ecological adaptations in extreme environments.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Kwoniella olivae</i> f.a. sp. nov., a novel basidiomycetous yeast species associated with olive ecosystems.","authors":"Michele Avesani, Claudia Carvalho, Cleo Gertrud Conacher, Heidi Christa Steffen, Alfred Botha, Giacomo Zapparoli, Jose Paulo Sampaio","doi":"10.1099/ijsem.0.006916","DOIUrl":"https://doi.org/10.1099/ijsem.0.006916","url":null,"abstract":"<p><p>Three yeast strains of the basidiomycetous genus <i>Kwoniella</i> (subphylum Agaricomycotina; class Tremellomycetes; subclass Tremellomycetidae) were isolated from samples of olive oil collected in northern Italy. Analysis of the D1/D2 domains of the 26S rRNA gene (LSU) and of the complete internal transcribed spacer (ITS) region indicated that these strains displayed high sequence similarity to three other strains isolated from insect frass in olive trees in South Africa, suggesting that they are conspecific. Phylogenetic analyses based on LSU and ITS sequences placed these six strains in a well-supported clade closely related to <i>Kwoniella newhampshirensis</i>, <i>Kwoniella shandongensis</i> and <i>Kwoniella lonicerae</i> with sequence divergence of 1.2-1.6% (7-9 nucleotide substitutions) in the D1/D2 domains and 1.8-6.4% (12-37 nucleotide substitutions) in the ITS regions. Based on these results and on phenotypic analyses, a novel species, <i>Kwoniella olivae</i> f.a. sp. nov. PYCC 9971^T (=CBS 18666^T), is proposed. The MycoBank number is MB 860006.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}