Pseudomonas lyxosi sp. nov., Pseudomonas arabinosi sp. nov. and Pseudomonas frigoris sp. nov., isolated from glaciers.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-06-01 DOI:10.1099/ijsem.0.006799

Chan Zhao, Yu-Hua Xin, Qing Liu

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Abstract

Three Gram-stain-negative, rod-shaped, motile bacterial strains, each with a single polar flagellum, designated LB3P38^T, LT1P18^T and ZB1P45^T, were isolated from glacier samples on the Tibetan Plateau, P.R. China. These strains grew at temperatures ranging from -2 to 32 °C (optimum 25-30 °C) and at pH values of 5.0-11.0 (optimum pH 7.0). The similarities of 16S rRNA gene sequences among these strains ranged from 99.67% to 99.93%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed their affiliation with the genus Pseudomonas. Phylogenomic analysis positioned these strains in close relation to Pseudomonas svalbardensis PMCC 200367^T and Pseudomonas frederiksbergensis LMG 19851^T. Average nucleotide identity values and digital DNA-DNA hybridization values between these strains and other type strains of the genus Pseudomonas were below 94.6% and 63.4%, respectively. The predominant fatty acids identified in these strains were C_16:0, C_17:0 cyclo and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c). Based on the combined phenotypic and phylogenetic evidence, each strain is proposed to represent a novel species within the genus Pseudomonas, with the names Pseudomonas lyxosi sp. nov. (type strain=LB3 P38^T=CGMCC 1.11284^T=JCM 37126^T), Pseudomonas arabinosi sp. nov. (type strain=LT1 P18^T=CGMCC 1.11310^T=JCM 37131^T) and Pseudomonas frigoris sp. nov. (type strain=ZB1 P45^T=CGMCC 1.23235^T=JCM 37141^T) proposed.

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从冰川中分离出的溶血性假单胞菌、阿拉伯假单胞菌和冷性假单胞菌。

从青藏高原的冰川样品中分离到3株革兰氏阴性杆状活动菌，每株菌均有单极鞭毛，分别命名为LB3P38T、LT1P18T和ZB1P45T。这些菌株的生长温度为-2至32℃（最适25-30℃），pH值为5.0-11.0（最适pH 7.0）。各菌株间16S rRNA基因序列相似性在99.67% ~ 99.93%之间。基于16S rRNA基因序列的系统发育分析证实了它们与假单胞菌属的亲缘关系。系统基因组分析表明，这些菌株与斯瓦尔巴登假单胞菌PMCC 200367T和弗雷德里克假单胞菌LMG 19851T亲缘关系密切。这些菌株与假单胞菌属其他类型菌株的平均核苷酸一致性值和数字DNA-DNA杂交值分别低于94.6%和63.4%。在这些菌株中鉴定出的优势脂肪酸为C16:0、C17:0 cyclo和总特征3 （C16:1 ω7c和/或C16:1 ω6c）。根据表型和系统发育的综合证据，每个菌株都代表假单胞菌属中的一个新种，分别命名为11月假单胞菌（型菌株=LB3 P38T=CGMCC 1.11284T=JCM 37126T）、11月阿拉伯假单胞菌（型菌株=LT1 P18T=CGMCC 1.11310T=JCM 37131T）和11月冷假单胞菌（型菌株=ZB1 P45T=CGMCC 1.232335 t =JCM 37141T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.