Arcobacter iocasae sp. nov., a bacterium isolated from the gill homogenate of the mussel Gigantidas platifrons in a cold seep.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-06-01 DOI:10.1099/ijsem.0.006818

Huan Zhang, Shi Jin, Hao Wang, Fei Li, Zhaoshan Zhong, Yang Guo, Jia Luo, Ziyun Ye, Chao Lian, Minxiao Wang, Chaolun Li

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Abstract

A novel Gram-negative, oxidase- and catalase-positive, rod-shaped bacterium, designated strain KX21116^T, was isolated from the mussel Gigantidas platifrons collected from a cold seep field in the South China Sea. Strain KX21116^T grew optimally at 28 °C, pH 6.0 with 3% (w/v) NaCl, under aerobic and microaerobic conditions. Its genome size was 3.16 Mb, with a G+C content of 28.4 mol%. The 16S rRNA sequences revealed that strain KX21116^T was closely related to Arcobacter nitrofigilis DSM 7299^T (98.77% gene sequence similarity) and Arcobacter acticola AR-13^T (95.58%). Phylogenetic and phylogenomic analysis revealed that strain KX21116^T clustered with the type species of the genus Arcobacter, with A. nitrofigilis DSM 7299^T as its nearest neighbour. The genomic average nucleotide identity (orthoANI) values between strain KX21116^T and A. nitrofigilis DSM 7299^T were 92.74%, while the in silico DNA-DNA hybridization (GGDC) values of the two strains were 48.8%. The predominant fatty acids are C_16:0, C_16:1 ω7c/C1_6:1 ω6c and C_18:1 ω7c/C1_8:1 ω6c. Based on a comparative analysis of phylogenetic, phylogenomic, phenotypic and chemotaxonomic characteristics, strain KX21116^T represents a novel species of the genus Arcobacter, for which the name Arcobacter iocasae sp. nov. is proposed. The type strain is KX21116^T (=MCCC 1K08505^T=KCTC 92900^T=JCM 35939^T).

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古干酪古杆菌，一种在冷水中从平台贻贝的鳃匀浆中分离出来的细菌。

从中国南海某海域采集的巨盘贻贝（Gigantidas platifrons）中分离到一株革兰氏阴性、氧化酶和过氧化氢酶阳性的新型杆状细菌，命名为KX21116T。菌株KX21116T在28℃、pH 6.0、3% (w/v) NaCl、好氧和微氧条件下生长最佳。基因组大小为3.16 Mb， G+C含量为28.4 mol%。16S rRNA序列分析显示，菌株KX21116T与nitrofigilis Arcobacter DSM 7299T和acticola Arcobacter AR-13T亲缘关系较近（基因序列相似性为98.77%）。系统发育和系统基因组分析表明，菌株KX21116T与Arcobacter属的模式种聚集在一起，与A. nitrofigilis DSM 7299T最接近。菌株KX21116T与硝基拟南芥DSM 7299T的基因组平均核苷酸同源性（orthoANI）值为92.74%，两者的DNA-DNA杂交（GGDC）值为48.8%。主要脂肪酸为C16:0、C16:1 ω7c/C16:1 ω6c和C18:1 ω7c/C18:1 ω6c。基于系统发育、系统基因组学、表型和化学分类特征的比较分析，菌株KX21116T代表了Arcobacter属的一个新种，建议将其命名为Arcobacter iocasae sp. 11。型应变为KX21116T （=MCCC 1K08505T=KCTC 92900T=JCM 35939T）。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.