International journal of systematic and evolutionary microbiology最新文献

{"title":"Unveiling hidden diversity within <i>Arcobacter cryaerophilus:</i> a description of <i>Arcobacter crypticus</i> sp. nov., <i>Arcobacter occultus</i> sp. nov. and <i>Arcobacter thermotolerans</i> sp. nov., and an emended description of <i>Arcobacter cryaerophilus</i>.","authors":"Lore Heyvaert, Charlotte Peeters, Kurt Houf, Stephen L W On, Peter Vandamme","doi":"10.1099/ijsem.0.007333","DOIUrl":"https://doi.org/10.1099/ijsem.0.007333","url":null,"abstract":"<p><p><i>Arcobacter cryaerophilus</i> is genomically heterogeneous with a type strain that is aberrant from most other strains in this species. Consequently, several infraspecific subdivisions have been introduced, including DNA-DNA hybridization groups, subgroups and genomovars. Recent comparative whole-genome sequence studies have corroborated and extended this heterogeneity and revealed the presence of four species among historical strains and new isolates which are now classified as <i>A. cryaerophilus</i>. The present study aimed to establish a simple formal classification of <i>A. cryaerophilus-</i>like bacteria that facilitate transparent communication by integrating genome sequence data of taxonomically well-characterized strains with biochemical characteristics. To this end, we propose three novel species: <i>Arcobacter crypticus</i> sp. nov. [with strain LMG 9065^T (=CCUG 12019^T) as the type strain], <i>Arcobacter occultus</i> sp. nov. [with strain LMG 29976^T (=CCUG 17808^T) as the type strain] and <i>Arcobacter thermotolerans</i> sp. nov. [with LMG 10829^T (=ATCC 49615^T) as the type strain]. This revised classification provides a robust basis for future investigations into the ecological roles and pathogenic potential of these taxa.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149238372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic, genomic and morphological characterization of <i>Rickettsia senegalensis</i> sp. nov., a novel <i>Rickettsia</i> species detected worldwide.","authors":"Clément Labarrere, Cheikh Tidiane Houmenou, Pierre-Edouard Fournier, Florence Fenollar, Oleg Mediannikov","doi":"10.1099/ijsem.0.007294","DOIUrl":"10.1099/ijsem.0.007294","url":null,"abstract":"<p><p>Members of the genus <i>Rickettsia</i> are widespread obligate intracellular bacteria with high medical and veterinary relevance. While <i>Rickettsia senegalensis</i> was previously isolated from cat fleas, <i>Ctenocephalides felis</i>, in Senegal, a comprehensive polyphasic characterization is required to validly publish its name. This study aims to provide the first detailed phenotypic, ultrastructural and genomic characterization of the type strain PU01-02ᵀ. Cultivation assays demonstrated that strain PU01-02ᵀ grows aerobically in XTC-2, SF9 and LD652 cell lines at 28 °C in a CO₂-free atmosphere. Ultrastructural examination via scanning electron microscopy using a backscattered electron detector confirmed its free cytoplasmic localization and a typical rickettsial morphology characterized by rod-shaped cells ~1.5 µm in length and 0.3 µm in width. Whole-genome sequencing yielded a 1.62 Mb genome with a G+C content of 33.2%, and three plasmids were detected. Genomic analyses confirmed its status as a distinct species, placing it within the transitional <i>Rickettsia</i> group, specifically inside the <i>Rickettsia felis</i> cluster. A review of epidemiological data revealed that rickettsial genes identical to those of <i>R. senegalensis</i> had already been identified in several haematophagous arthropods, including fleas and ticks parasitizing various hosts such as cats, dogs, opossums and rodents worldwide. It has also been detected in cat tissues, suggesting a potential host-pathogen association.Based on these comprehensive characterization data, we formally propose <i>R. senegalensis</i> sp. nov. as a new species, with PU01-02ᵀ (=CSUR R184ᵀ =DSM 28250ᵀ) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13649058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the species boundaries for the genus <i>Kribbella</i> and taxonomic description of <i>Kribbella polygonati</i> sp. nov., and <i>Kribbella eucommiae</i> sp. nov., from rhizosphere soil of different plants, with proposal of <i>Kribbella ginsengisoli</i> as a later heterotypic synonym of <i>Kribbella koreensis</i>.","authors":"Ming-Yue Li, Kai-Qin Li, Yan Xiao, Jian Gao","doi":"10.1099/ijsem.0.007314","DOIUrl":"https://doi.org/10.1099/ijsem.0.007314","url":null,"abstract":"<p><p>Now, 95-96% average nucleotide identity (ANI) and 70% digital DNA-DNA hybridization (dDDH) are regarded as the gold standards for delineating prokaryotic species. However, during the process of identifying <i>Kribbella</i> strains, we found that the contradictory phenomena may sometimes occur using 95-96% ANI and 70% dDDH as the standards, respectively; the <i>gyrB</i> or concatenated <i>gyrB-rpoB-recA-relA-atpD</i> genetic distances currently used to replace ANI or dDDH are insufficient to serve as thresholds for delineating <i>Kribbella</i> species. Contraposing these problems, we re-evaluated the correlations between ANI and dDDH, between <i>gyrB</i> genetic distance and dDDH and between <i>gyrB-rpoB-recA-relA-atpD</i> genetic distance and dDDH from 33 <i>Kribbella</i> species. Based on our analysis results, a 96.4% ANIm value or a 96.3% ANIb value can be considered as the boundary value for delineating <i>Kribbella</i> species; a 0.009 <i>gyrB</i> or 0.010 <i>gyrB-rpoB-recA-relA-atpD</i> genetic-distance value can also serve as a reliable threshold for species delineation within the genus <i>Kribbella</i>. Meanwhile, two novel actinobacterial strains, GL6^T and HUAS MG21^T, isolated from the rhizosphere soil of different plants, respectively, were characterized using a polyphasic approach. Based on phenotypic and chemotaxonomic characteristics, as well as phylogeny and genomic correlation, strains GL6^T and HUAS MG21^T represent two novel <i>Kribbella</i> species, for which the names <i>Kribbella polygonati</i> sp. nov. and <i>Kribbella eucommiae</i> sp. nov. are proposed, respectively. Furthermore, the comparative genomic analysis suggested that <i>Kribbella ginsengisoli</i> and <i>Kribbella koreensis</i> should be considered the same species. According to the principle of priority, it is proposed that <i>K. ginsengisoli</i> Cui <i>et al</i>. 2010 is a later heterotypic synonym of <i>K. koreensis</i> (Lee <i>et al</i>. 2000) Sohn <i>et al</i>. 2003.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149286744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation list no. 231: valid publication of new names and new combinations effectively published outside the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.007238","DOIUrl":"10.1099/ijsem.0.007238","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 9","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13656799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149109238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Pantoea citricola</i> sp. nov. and <i>Pantoea folii</i> sp. nov., two bacteria with plant-growth-promoting traits isolated from the phyllosphere of honey pomelo (<i>Citrus maxima</i>).","authors":"Jun Zhang, Xiang Rong, Qingying Chen, Jiali Li, Guang-Da Feng, Honghui Zhu, Songzhen Yang","doi":"10.1099/ijsem.0.007319","DOIUrl":"https://doi.org/10.1099/ijsem.0.007319","url":null,"abstract":"<p><p>Two yellow-pigmented, Gram-negative, facultatively anaerobic, motile and rod-shaped bacterial strains, designated DMYE4^T and HJE1^T, were isolated from healthy leaves of honey pomelo (<i>Citrus maxima</i>) in Dabu County, Guangdong Province, PR China. Both strains could produce siderophore and solubilize inorganic phosphate, implicating promising potential in promoting plant growth. Strain DMYE4^T could grow at 15‒37 °C (optimum at 28‒30 °C) and pH 5.0‒8.0 (optimum, pH 5.0) in the presence of 0‒7.0% (w/v) NaCl (optimum, 1.0-3.0%), while strain HJE1^T grew at 10‒37 °C (optimum, 28‒30 °C) and pH 5.0‒8.0 (optimum, pH 5.0) in the presence of 0‒10.0% (w/v) NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene and genome sequences supported that strains DMYE4^T and HJE1^T belonged to the genus <i>Pantoea</i> and were most closely related to <i>Pantoea vagans</i> LMG 24199^T and <i>Pantoea rodasii</i> LMG 26273^T, respectively. The genome-derived average nucleotide identity values between the novel strains and their closely related type strains were 87.72-94.59% (DMYE4^T) and 83.52-88.86% (HJE1^T), and the corresponding digital DNA-DNA hybridization values were 33.9-58.6% and 27.4-36.7%, respectively, which were all below the suggested thresholds recommended for new prokaryotic taxa delimitation. Both strains contained ubiquinone 8 as the predominant respiratory quinone, and their major fatty acids (>10% of the total amount) were C_16 : 0, summed feature 3 (C_16 : 1 <i> ω</i>7c and/or C_16 : 1 <i> ω</i>6<i>c</i>) and summed feature 8 (C_18 : 1 <i> ω</i>7<i>c</i> and/or C_18 : 1 <i> ω</i>6<i>c</i>). The genomic DNA G+C contents of strains DMYE4^T and HJE1^T were 55.05 and 53.86 mol%, respectively. Based on this polyphasic taxonomic study, strains DMYE4^T and HJE1^T represent two distinct novel species within the genus <i>Pantoea</i>, for which the names <i>Pantoea citricola</i> sp. nov. and <i>Pantoea folii</i> sp. nov. are proposed, respectively. The type strains are DMYE4^T (=GDMCC 1.4184^T=JCM 36484^T) and HJE1^T (=GDMCC 1.4186^T=JCM 36485^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Yunzhangomyces sedi</i> sp. nov., a basidiomycetous yeast species isolated from phylloplane.","authors":"Yan Cui, Chun-Yue Chai, Feng-Li Hui","doi":"10.1099/ijsem.0.007325","DOIUrl":"https://doi.org/10.1099/ijsem.0.007325","url":null,"abstract":"<p><p>Two yeast strains, CICC 33533 and NYNU 26815, belonging to the basidiomycetous yeast genus <i>Yunzhangomyces</i>, were isolated from the leaf surfaces of <i>Sedum aizoon</i> and <i>Sorbus alnifolia,</i> respectively, collected in the Baotianman Nature Reserve, Henan Province, China. Phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene, together with detailed phenotypic characterization, indicated that these two strains, along with an additional strain NRP040 isolated from a plant in Japan, represent a novel asexual species of the genus <i>Yunzhangomyces</i> in the order Exobasidiales. The name <i>Yunzhangomyces sedi</i> sp. nov. (MycoBank MB 863876) is, therefore, proposed with strain CICC 33533 designated as the holotype. The novel species differs from its closely related species, <i>Yunzhangomyces orchidis</i>, <i>Yunzhangomyces clavatus</i>, <i>Yunzhangomyces cylindricus</i>, <i>Yunzhangomyces nandinae</i> and <i>Yunzhangomyces scirpi</i> by 24-32 nucleotide substitutions (4.2-5.7%) in the D1/D2 domain. In the ITS region, the novel species also exhibited more than 23 nucleotide mismatches (6.4%) from <i>Y. orchidis</i>, <i>Y. clavatus</i>, <i>Y. cylindricus</i> and <i>Y. nandinae</i>. The ITS sequence of <i>Y. scirpi</i> was not available for comparison. Minor physiological differences were also observed: the novel species was able to assimilate galactitol, thereby differentiating it from the closely related yeast-like species <i>Y. orchidis</i>, <i>Y. clavatus</i>, <i>Y. cylindricus</i> and <i>Y. nandinae</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149181583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of <i>Corynebacterium</i> species isolated from nasal swabs of healthy volunteers: description of <i>Corynebacterium nasorum</i> sp. nov., <i>Corynebacterium tardum</i> sp. nov., <i>Corynebacterium tuingiense</i> sp. nov., <i>Corynebacterium limaceinasi</i> sp. nov. and <i>Corynebacterium kefirresidentii</i> sp. nov.","authors":"Matthias Otto, Laura Camus, Tobias Busche, Judith Bünte, Andreas Hütten, Marcus Persicke, Levin Joe Klages, Jörn Kalinowski, Simon Heilbronner, Christian Rückert-Reed","doi":"10.1099/ijsem.0.007292","DOIUrl":"https://doi.org/10.1099/ijsem.0.007292","url":null,"abstract":"<p><p>Members of the genus <i>Corynebacterium</i> are common constituents of the human nasal microbiota but their cultivable diversity remains incompletely represented in taxonomic and diagnostic databases. Ten coryneform strains isolated from nasal swabs of seven healthy adults were characterized using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), whole-genome sequencing, phylogenomic inference, overall genome relatedness indices, phenotypic testing and chemotaxonomic analyses. MALDI-TOF MS initially assigned most strains to <i>Corynebacterium tuberculostearicum</i> or <i>Corynebacterium accolens</i>, whereas genome-based analyses resolved the strains into five distinct species-level lineages. The lineages represented by strains LaCa116^T, LaCa142^T and LaCa191^T were clearly differentiated from their closest related type strains by average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values below accepted species-level thresholds. Strains LaCa7^T and LaCa117^T represented closely related but distinguishable genomic lineages, with an ANI value of 95.9% and a dDDH value of 66.4%. Strain LaCa117^T was closely related to strain SB, to which the effectively but not validly published name '<i>Corynebacterium kefirresidentii</i>' had previously been applied. Phenotypic and chemotaxonomic characteristics supported the genomic groupings and placement of the strains within the genus <i>Corynebacterium</i>. These findings reveal previously unrecognized diversity among cultivable nasal corynebacteria and demonstrate that routine MALDI-TOF MS identification may be unreliable when suitable reference spectra are absent. Based on the integrated taxonomic evidence, the names <i>Corynebacterium nasorum</i> sp. nov. (type strain LaCa7^T=DSM 119349^T=LMG 33882^T), <i>Corynebacterium kefirresidentii</i> sp. nov. (type strain LaCa117^T=DSM 119343^T=LMG 33886^T), <i>Corynebacterium tuingiense</i> sp. nov. (type strain LaCa142^T=DSM 119342^T=LMG 33884^T), <i>Corynebacterium limaceinasi</i> sp. nov. (type strain LaCa191^T=DSM 119341^T=LMG 33883^T) and <i>Corynebacterium tardum</i> sp. nov. (type strain LaCa116^T=DSM 119344^T=LMG 33885^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149267573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Corynebacterium nasorum</i> sp. nov. and <i>Corynebacterium hallucis</i> sp. nov. isolated from human nasal passages and skin.","authors":"Elena B Popowitch, Tommy H Tran, Isabel F Escapa, Eeshta Bhatt, Alp K Sozat, Nashwa Ahmed, Clayton Deming, Ari Q Roberts, Nisc Comparative Sequencing Program, Julia A Segre, Heidi H Kong, Sean Conlan, Katherine P Lemon, Matthew S Kelly","doi":"10.1099/ijsem.0.007305","DOIUrl":"10.1099/ijsem.0.007305","url":null,"abstract":"<p><p>Strains of two novel <i>Corynebacterium</i> species were cultured from samples of human nostrils and skin collected in the USA and Botswana. These strains demonstrated growth on Columbia Colistin-Nalidixic Acid agar with 5% sheep blood and in liquid media (brain-heart infusion and tryptic soy broth) supplemented with Tween 80, a source of the fatty acid oleic acid. Cells were Gram-positive, non-spore-forming, non-motile bacilli that showed catalase but not oxidase activity. Major fatty acids in both of these species were C_18 : 1 ω9c (oleic acid), C_16 : 0 (palmitic acid) and C_18 : 0 (stearic acid). Analysis of the 16S rRNA gene sequences identified these strains as belonging to the genus <i>Corynebacterium</i> (family <i>Corynebacteriaceae</i>). Whole-genome sequencing revealed that these strains formed distinct branches on a phylogenomic tree, with average nt identities of <95% relative to all previously described species. These results indicate that these strains represent novel species of <i>Corynebacterium</i>, for which we propose the names <i>Corynebacterium hallucis</i> sp. nov., with the type strain CTNIH22^T (=ATCC TSD-435^T=DSM 117774^T), and <i>Corynebacterium nasorum</i> sp. nov., with the type strain KPL3804^T (=ATCC TSD-439^T=DSM 117767^T). These data also indicate that '<i>Corynebacterium kefirresidentii</i>' is designated <i>C. nasorum</i> sp. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13690174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149244475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Sphingobium fluminis</i> sp. nov. and <i>Sphingobium anseongense</i> sp. nov.<i>,</i> isolated from riverbank soil.","authors":"Ju-Young Hong, Jung-Hun Jo, Se-Yoon Chun, Wan-Taek Im","doi":"10.1099/ijsem.0.007300","DOIUrl":"https://doi.org/10.1099/ijsem.0.007300","url":null,"abstract":"<p><p>Two novel Gram-stain-negative, aerobic, heterotrophic, motile bacterial strains, designated AN558^T and AN641^T, were isolated from riverbank soil in South Korea. To determine their taxonomic positions, the strains were characterized using a polyphasic taxonomic approach based on 16S rRNA gene phylogeny, phenotypic and chemotaxonomic characteristics and genome-based analyses. Phylogenetic and genome-based analyses revealed that the two novel isolates, AN558^T and AN641^T, belong to the genus <i>Sphingobium</i>. AN558^T showed the highest 16S rRNA gene similarity (97.3%) with <i>Sphingobium yanoikuyae</i> ATCC 51230^T. In addition, AN641^T showed 97.6% 16S rRNA gene similarity with <i>Sphingobium arseniciresistens</i> H39-3-25^T. The genome of strains AN558^T and AN641^T consisted of 3,717,653 and 3,426,895 bp, with DNA G+C content of 62.8 and 63.6 mol%, respectively. The average nucleotide identity between the two strains and other related species was below 79.1%, and the digital DNA-DNA hybridization values with closely related species were below 22.4%, both lower than the species delineation threshold. Strains AN558^T and AN641^T contained ubiquinone Q-10 as the major respiratory quinone and exhibited a polyamine pattern, with spermidine as the predominant polyamine. The major fatty acids were characterized by C_16 : 0, C_16 : 1 <i> ω7</i>c<i>/</i>C1_6 : 1 <i> ω6</i>c (summed feature 3) and C_18 : 1 <i> ω7</i>c<i>/</i>C1_8 : 1 <i> ω6</i>c (summed feature 8). The major polar lipids of AN558^T and AN641^T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid and phosphatidylcholine. Therefore, AN558^T and AN641^T represent novel species of the genus <i>Sphingobium</i>, for which the names <i>Sphingobium fluminis</i> sp. nov. (type strain AN558^T=KACC 23515^T=LMG 33415^T) and <i>Sphingobium anseongense</i> sp. nov. (type strain AN641^T=KACC 23516^T=LMG 33416^T) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149193695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Asticcacaulis aquihabitans</i> sp. nov., a novel psychrotrophic member of the phylum <i>Pseudomonadota</i> isolated from Lake Michigan potable water.","authors":"Richard A Albert, Jonathan Roecker, Alex Burmesch, McKayla Zastrow, Alana L Dwenger, Anthony Kappell, Nicholas Benn, Daniel Zitomer, Lauren Salvitti, Paul A Lawson","doi":"10.1099/ijsem.0.007302","DOIUrl":"https://doi.org/10.1099/ijsem.0.007302","url":null,"abstract":"<p><p>Six strains of a Gram-stain-negative, psychrotrophic, aerobic, rod-shaped, motile bacterium were recovered from water samples of the Port Washington, WI, USA, potable water system. Strain 201^T was designated as the type strain. Phylogenetic analysis based upon the 16S rRNA gene sequence showed that strain 201^T represents a novel species belonging to the genus <i>Asticcacaulis</i>. Strain 201^T grows between 5 and 40 °C and has a salt tolerance range of 0-1.5%. The primary quinone was ubiquinone Q-10 (98.8%) with a minor amount of Q-9 (1.2%), while the polar lipids included three glycolipids, one unidentified lipid and phosphatidylglycerol. The predominant cellular fatty acids are C_16 : 0 and sum in feature 8 (C_{18:1 ω9c}/C_{18:1 ω7c}/C_{18:1 ω6c}). The genomic DNA G+C content of strain 201^T was 58.8 mol%, with a genome size of 4.7 Mb. The average nt identity and digital DNA-DNA hybridization between strain 201^T and its closest phylogenetic relative <i>Asticcacaulis taihuensis</i> CGMCC 1.3431^T were 77 and 20.7%, respectively, well below the values used to delineate species. Based upon phenotypic, genomic and phylogenetic data, strain 201^T represents a novel species of the genus <i>Asticcacaulis</i>, for which we propose the name <i>Asticcacaulis aquihabitans</i>. The type strain is 201^T (=LMG 33251^T=NRRL B-65659^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"76 10","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}