Valentina Carrasco, Matías Vargas-Reyes, Sebastián Lagos-Moraga, Claudio Dietz-Vargas, Daniela Allendes-Ormazábal, Juan Meza-Inzunza, Fernanda Rojas-Morales, Sebastián Durán-Villegas, Felipe Valenzuela-Ibaceta, Ma Ángeles Cabrera, José M Pérez-Donoso
{"title":"<i>Rhodococcus navarretei</i> sp. nov. and <i>Pseudarthrobacter quantipunctorum</i> sp. nov., two novel species with the ability to biosynthesize fluorescent nanoparticles, isolated from soil samples at Union Glacier, Antarctica.","authors":"Valentina Carrasco, Matías Vargas-Reyes, Sebastián Lagos-Moraga, Claudio Dietz-Vargas, Daniela Allendes-Ormazábal, Juan Meza-Inzunza, Fernanda Rojas-Morales, Sebastián Durán-Villegas, Felipe Valenzuela-Ibaceta, Ma Ángeles Cabrera, José M Pérez-Donoso","doi":"10.1099/ijsem.0.006536","DOIUrl":"10.1099/ijsem.0.006536","url":null,"abstract":"<p><p>Two Gram-stain-positive bacterial strains, EXRC-4A-4<sup>T</sup> and RC-2-3<sup>T</sup>, were isolated from soil samples collected at Union Glacier, Antarctica. Based on 16S rRNA gene sequence similarity, strain EXRC-4A-4<sup>T</sup> was identified as belonging to the genus <i>Rhodococcus</i>, and strain RC-2-3<sup>T</sup> to the genus <i>Pseudarthrobacter</i>. Further genomic analyses, including average nucleotide identity and digital DNA-DNA hybridization, suggested that these strains represent new species. Strain EXRC-4A-4<sup>T</sup> exhibited growth at temperatures ranging from 4 to 28 °C (optimum between 20 and 28 °C), at pH 5.0-9.0 (optimum, pH 6.0), and in the presence of 0-5.0% NaCl (optimum between 0 and 1% NaCl). Strain RC-2-3<sup>T</sup> grew at 4-28 °C (optimum growth at 28 °C), pH 6.0-10 (optimum, pH 7.0) and in the presence of 0-5.0% NaCl (optimum, 1% NaCl). The fatty acid profile of EXRC-4A-4<sup>T</sup> was dominated by C<sub>17:1 ω-7</sub>, while that of RC-2-3<sup>T</sup> was dominated by anteiso-C<sub>15 : 0</sub>. The draft genome sequences revealed a DNA G+C content of 64.6 mol% for EXRC-4A-4<sup>T</sup> and 65.8 mol% for RC-2-3<sup>T</sup>. Based on this polyphasic study, EXRC-4A-4<sup>T</sup> and RC-2-3<sup>T</sup> represent two novel species within the genera <i>Rhodococcus</i> and <i>Pseudarthrobacter</i>, respectively. We propose the names <i>Rhodococcus navarretei</i> sp. nov. and <i>Pseudarthrobacter quantipunctorum</i> sp. nov. The type strains are <i>Rhodococcus navarretei</i> EXRC-4A-4<sup>T</sup> and <i>Pseudarthrobacter quantipunctorum</i> RC-2-3<sup>T</sup>. These strains have been deposited deposited in the CChRGM and BCCM/LMG culture collections with entry numbers RGM 3539/LMG 33621 and RGM 3538/LMG 33620, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Emendation of Appendix 9 of the International Code of Nomenclature of Prokaryotes to regulate the use of connecting vowels in compound names after stems ending in the same vowel.","authors":"Aharon Oren","doi":"10.1099/ijsem.0.006535","DOIUrl":"10.1099/ijsem.0.006535","url":null,"abstract":"<p><p>Following a proposal to emend Appendix 9 of the International Code of Nomenclature of Prokaryotes with guidelines to regulate the use of connecting vowels in compound names after stems ending in the same vowel, I here report the outcome of the ballot on this proposal by the members of the International Committee on Systematics of Prokaryotes. The new guidelines to be incorporated in Appendix 9 are presented.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Polymorphospora lycopeni</i> sp. nov., a lycopene-producing actinomycetes isolated from lakeside soil sample of Baiyangdian.","authors":"Jiashan Liu, Shilong Li, Xiaomin Duan, Hongmei Li, Xiumin Zhang","doi":"10.1099/ijsem.0.006543","DOIUrl":"https://doi.org/10.1099/ijsem.0.006543","url":null,"abstract":"<p><p>A novel actinomycetes producing lycopene, designated HBU208002<sup>T</sup>, was isolated from a lakeside soil sample collected in Baiyangdian, located in Xiong'an New Area of China, and its taxonomic position was investigated by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the strain HBU208002<sup>T</sup> fell within the genus <i>Polymorphospora</i> and was closely related to <i>Polymorphospora rubra</i> JCM 14904<sup>T</sup> (99.73% identity). However, the average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH) and average amino acid identity (AAI) values between the strain HBU208002<sup>T</sup> and <i>P. rubra</i> JCM 14904<sup>T</sup> were 91.78, 44.7 and 91.6%, respectively, which were lower than the ANI (95-96%), DDH (>70%) and AAI (>95%) thresholds of prokaryotic microbial defined species. The predominant fatty acids of the strain HBU208002<sup>T</sup> were iso-C<sub>16:0</sub>, C<sub>17:1</sub> ω8c. The menaquinones of the strain HBU208002<sup>T</sup> were MK-8(H<sub>8</sub>) and MK9(H<sub>4</sub>), while those of <i>P. rubra</i> JCM 14904<sup>T</sup> were MK-10(H<sub>6</sub>), MK-10(H<sub>4</sub>), MK-9(H<sub>6</sub>) and MK-9(H<sub>4</sub>). Meanwhile, some phenotypic characterizations and antibacterial activities distinguished the strain HBU208002<sup>T</sup> from <i>P. rubra</i> JCM 14904<sup>T</sup>. The strain HBU208002<sup>T</sup> exhibited inhibitory effects on <i>Fusarium graminearum</i>, <i>Fusarium verticillioides</i> and <i>Botrytis cinerea</i>, but <i>P. rubra</i> JCM 14904<sup>T</sup> had no activity. Therefore, the strain HBU208002<sup>T</sup> should be assigned as representing a novel species of the genus <i>Polymorphospora</i>, for which the name <i>Polymorphospora lycopeni</i> was proposed. The type strain is HBU208002<sup>T</sup> (=KCTC49833<sup>T</sup> = GDMCC4.236<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuya Hatakeyama, Sayaka Mino, Mana Mizobata, Mako Takada, Jiro Tsuchiya, Shogo Yamaki, Yasuhiro Ando, Tomoo Sawabe, Ken Takai
{"title":"<i>Hydrogenimonas leucolamina</i> sp. nov., a hydrogen- and sulphur-oxidizing mesophilic chemolithoautotroph isolated from a deep-sea hydrothermal vent chimney at the Suiyo Seamount in the Western Pacific Ocean.","authors":"Shuya Hatakeyama, Sayaka Mino, Mana Mizobata, Mako Takada, Jiro Tsuchiya, Shogo Yamaki, Yasuhiro Ando, Tomoo Sawabe, Ken Takai","doi":"10.1099/ijsem.0.006553","DOIUrl":"10.1099/ijsem.0.006553","url":null,"abstract":"<p><p>A novel mesophilic bacterium, strain SS33<sup>T</sup>, was isolated from a deep-sea hydrothermal vent chimney at Suiyo Seamount, Izu-Bonin Arc, Western Pacific Ocean. The cells of strain SS33<sup>T</sup> were motile short rods with a single polar flagellum. The growth of strain SS33<sup>T</sup> was observed at the temperature range between 33 and 55 °C (optimum growth at 45 °C), at the pH range between 5.0 and 7.1 (optimum growth at pH 6.0) and in the presence of between 2.0 and 4.5% (w/v) NaCl [optimum growth at 3.5% (w/v)]. Strain SS33<sup>T</sup> was a facultative anaerobic chemolithoautotroph using molecular hydrogen and elemental sulphur as the sole electron donor. Nitrate, nitrous oxide, sulphate, elemental sulphur and molecular oxygen were capable of serving as the sole electron acceptor. Phylogenetic analysis based on 16S rRNA gene sequences placed strain SS33<sup>T</sup> in the genus <i>Hydrogenimonas</i> belonging to the class <i>Epsilonproteobacteria</i>. The closely related species of strain SS33<sup>T</sup> were <i>Hydrogenimonas urashimensis</i> SSM-Sur55<sup>T</sup> (95.96%), <i>Hydrogenimonas thermophila</i> EP1-55-1%<sup>T</sup> (95.75%) and <i>Hydrogenimonas cancrithermarum</i> ISO32<sup>T</sup> (95.24%). According to the taxonomic and physiological characteristics, it is proposed that strain SS33<sup>T</sup> was classified into a novel species of genus <i>Hydrogenimonas</i>, <i>Hydrogenimonas leucolamina</i> sp. nov., with SS33<sup>T</sup> (=JCM 39184<sup>T</sup> =KCTC 25253<sup>T</sup>) as the type strain. Furthermore, the genome comparison of <i>Epsilonproteobacteria</i> revealed that their [NiFe] hydrogenase genes belonging to Group 1b could be divided into two phylogenetic lineages and suggested that the reverse gyrase gene has been lost after division to the genus <i>Hydrogenimonas</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meng-Han Huang, Dao-Feng Zhang, Hong-Chuan Wang, Wei He, Xiao-Rui Song
{"title":"Description of <i>Rhodobacter flavimaris</i> sp. nov. and proposal of the genera <i>Paenirhodobacter</i>, <i>Sedimentimonas</i>, and <i>Sinirhodobacter</i> as synonyms of <i>Rhodobacter</i>.","authors":"Meng-Han Huang, Dao-Feng Zhang, Hong-Chuan Wang, Wei He, Xiao-Rui Song","doi":"10.1099/ijsem.0.006540","DOIUrl":"10.1099/ijsem.0.006540","url":null,"abstract":"<p><p>Two Gram-stain-negative, aerobic, ovoid to short rod-shaped bacterial strains, designated as WL0062<sup>T</sup> and WL0115, were isolated from coastal zone of the Yellow Sea, Jiangsu Province, PR China, respectively. Strain WL0062<sup>T</sup> grew optimally at 28 °C, pH 7.0-8.0 and with 1.0-3.0% (w/v) NaCl. Strain WL0115 grew optimally at 28 °C, pH 6.0-7.0 and with 1.0-3.0% (w/v) NaCl. In the bac120 tree, strains WL0062<sup>T</sup> and WL0115 clustered together with <i>Sedimentimonas flavescens</i> B57<sup>T</sup>. The respiratory quinone of both strains was ubiquinone-10. The major polar lipids of both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, glycolipid, phosphatidylmonomethylethanolamine, and one unidentified polar lipid. The major fatty acids of strain WL0062<sup>T</sup> were summed features 8 (C<sub>18 : 1</sub> <i> ω</i>6<i>c</i> and/or C<sub>18 : 1</sub> <i> ω</i>7<i>c</i>). The major fatty acids of strain WL0115 were summed features 8 (C<sub>18 : 1</sub> <i> ω</i>6<i>c</i> and/or C<sub>18 : 1</sub> <i> ω</i>7<i>c</i>), C<sub>18 : 0</sub>, iso-C<sub>17 : 1</sub> <i> ω</i>5<i>c</i> and C<sub>20 : 4</sub> <i> ω</i>6/9/12/15<i>c</i> (arachidonic acid). The G+C content of genomic DNA of strains WL0062<sup>T</sup> and WL0115 was 64.0 mol% in both of them. Combined with the analysis of average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization, strain WL0062<sup>T</sup> represents a novel species of the genus <i>Rhodobacter</i>, for which the name <i>Rhodobacter flavimaris</i> sp. nov is proposed. The type strain is WL0062<sup>T</sup> (=MCCC 1K06014<sup>T</sup>=JCM 34676<sup>T</sup>=GDMCC 1.2427<sup>T</sup>). Strain WL0115 (=MCCC 1K07531=JCM 35568=GDMCC 1.3088) should belong to the same species as <i>Sedimentimonas flavescens</i> B57<sup>T</sup>. In addition, on the basis of phylogenomic relationship and phenotypical characteristics, the genera <i>Paenirhodobacter</i>, <i>Sedimentimonas</i>, and <i>Sinirhodobacter</i> are proposed as synonyms of <i>Rhodobacter</i>.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taiki Katayama, Masaru K Nobu, Yoichi Kamagata, Hideyuki Tamaki
{"title":"<i>Fidelibacter multiformis</i> gen. nov., sp. nov., isolated from a deep subsurface aquifer and proposal of <i>Fidelibacterota</i> phyl. nov., formerly called Marine Group A, SAR406 or <i>Candidatus</i> Marinimicrobia.","authors":"Taiki Katayama, Masaru K Nobu, Yoichi Kamagata, Hideyuki Tamaki","doi":"10.1099/ijsem.0.006558","DOIUrl":"https://doi.org/10.1099/ijsem.0.006558","url":null,"abstract":"<p><p>A Gram-negative, obligatory anaerobic, chemoheterotrophic bacterium, designated strain IA91<sup>T</sup>, was isolated from sediments and formation water from deep aquifers in Japan. IA91<sup>T</sup> derives its peptidoglycan, energy and carbon from exogenous cell wall fragments, namely muropeptides, released from actively reproducing bacteria, and is dependent on other bacteria for cell wall formation, growth and even cell shape: IA91<sup>T</sup> is irregular rod-shaped but coccoids when muropeptide is absent. IA91<sup>T</sup> grew in a temperature range of 25-45 °C with optimum growth at 40 °C. IA91<sup>T</sup> utilized limited substrates, yeast extract, muropeptides and d-lactate. The major end products from yeast extract degradation were acetate, hydrogen and carbon dioxide. Co-cultivation with a hydrogen-scavenging methanogenic archaeon promoted IA91<sup>T</sup> growth. No anaerobic respiration with nitrate, nitrite, sulphate or Fe(III) was observed. The major cellular fatty acids are C16 : 0, C18 : 1 <i>trans</i>9, C18 : 0 and C17 : 0. The G+C content of the genomic DNA was 45.6 mol%. Phylogenetic analysis based on 16S rRNA gene and conserved protein sequences involved in replication, transcription and translation indicated that IA91<sup>T</sup> belonged to the candidate phylum Marine Group A (MG-A, SAR406 or <i>Ca</i>. Marinimicrobia) with no cultivated representatives. Based on the phenotypic and phylogenomic characteristics, a new genus and species, <i>Fidelibacter multiformis</i> gen. nov., sp. nov., is proposed for IA91<sup>T</sup> (= JCM 39387<sup>T</sup> = KCTC 25736<sup>T</sup>). In addition, a new bacterial phylum named <i>Fidelibacterota</i> phyl. nov. is proposed for the candidate phylum MG-A represented by <i>F. multiformis</i> and <i>Fidelibacteraceae</i> fam. nov., <i>Fidelibacterales</i> ord. nov. and <i>Fidelibacteria</i> classis nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyunji Lee, Inhyup Kim, Sunho Park, Haejin Woo, Subin Yook, Taegun Seo
{"title":"<i>Sphingomonas rustica</i> sp. nov. and <i>Sphingomonas agrestis</i> sp. nov., novel carotenoid-producing bacterial species isolated from farm soil.","authors":"Hyunji Lee, Inhyup Kim, Sunho Park, Haejin Woo, Subin Yook, Taegun Seo","doi":"10.1099/ijsem.0.006551","DOIUrl":"https://doi.org/10.1099/ijsem.0.006551","url":null,"abstract":"<p><p>Two yellow-pigmented novel strains, designated HF-S3<sup>T</sup> and HF-S4<sup>T</sup>, were isolated from farm soil in Paju, Republic of Korea. Cells of the two strains are characteristically Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and rod-shaped. Strain HF-S3<sup>T</sup> grew at 10-37 °C, while HF-S4<sup>T</sup> grew at 15-35 °C. Both strains grew at pH 5.0-12.0 and in NaCl concentrations (w/v) of 0-2.0%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that HF-S3<sup>T</sup> and HF-S4<sup>T</sup> belong to the genus <i>Sphingomonas</i>, with HF-S3<sup>T</sup> exhibiting 97.7, 97.6 and 97.4% similarity to <i>Sphingomonas cannabina</i> DM2-R-LB4<sup>T</sup>, <i>Sphingomonas leidyi</i> DSM 4733<sup>T</sup> and <i>Sphingomonas canadensis</i> FWC47<sup>T</sup>, respectively. Strain HF-S4<sup>T</sup> displayed 97.9, 97.7 and 97.6% similarity to <i>Sphingomonas psychrotolerans</i> Cra20<sup>T</sup>, <i>Sphingomonas gei</i> ZFGT-11<sup>T</sup> and <i>Sphingomonas naasensis</i> KIS18-15<sup>T</sup>, respectively. The DNA G+C contents of HF-S3<sup>T</sup> and HF-S4<sup>T</sup> were 67.0 and 66.5 mol%, respectively. The digital DNA-DNA hybridization and average nucleotide identity values among the novel and related type strains were 20.2-28.2% and 75.9-84.3%, respectively. They all contained C<sub>14:0</sub> 2-OH and C<sub>16:0</sub>, summed feature 8 (C<sub>18:1</sub> <i>ω</i>6<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>7<i>c</i>) as the major fatty acids and ubiquinone-10 as the predominant respiratory quinone. Strains HF-S3<sup>T</sup> and HF-S4<sup>T</sup> were found to produce carotenoid-type pigments. Based on polyphasic taxonomic analysis, the new isolates ostensibly represent two novel species of the genus <i>Sphingomonas</i>, with the proposed names <i>Sphingomonas rustica</i> sp. nov. and <i>Sphingomonas agrestis</i> sp. nov. for strains HF-S3<sup>T</sup> and HF-S4<sup>T</sup>, respectively. The <i>S. rustica</i> and <i>S. agrestis</i> type strains are HF-S3<sup>T</sup> (=KACC 23554<sup>T</sup> =TBRC 18352<sup>T</sup>) and HF-S4<sup>T</sup> (=KACC 23386<sup>T</sup> =TBRC 17899<sup>T</sup>), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142465548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samuel Breselge, Paolo Bellassi, Coral Barcenilla, Avelino Álvarez-Ordóñez, Lorenzo Morelli, Paul D Cotter
{"title":"<i>Bifidobacterium fermentum</i> sp. nov. and <i>Bifidobacterium aquikefiricola</i> sp. nov., isolated from water kefir.","authors":"Samuel Breselge, Paolo Bellassi, Coral Barcenilla, Avelino Álvarez-Ordóñez, Lorenzo Morelli, Paul D Cotter","doi":"10.1099/ijsem.0.006549","DOIUrl":"https://doi.org/10.1099/ijsem.0.006549","url":null,"abstract":"<p><p>Four strains, representing two novel <i>Bifidobacterium</i> species, were isolated from water kefir, a fermented beverage. 16S rRNA gene analysis suggested that the novel species share high identities (98.82-98.89%) with <i>Bifidobacterium aquikefiri</i> LMG 28769<sup>T</sup>. Complete genomes were assembled with a short- and long-read hybrid sequencing approach. In agreement with the 16S rRNA gene analysis, phylogenetics with 117 marker genes places the novel species closest to <i>B. aquikefiri</i> LMG 28769<sup>T</sup> as well. The isolates have average nucleotide identity (ANI) scores ranging from 81.46 to 84.84% and digital DNA-DNA hybridization (dDDH) scores from 23.9 to 38.5% with the closest related species, as well as ANI scores between the proposed new species of 80.50%, indicating that the isolates represent two novel species. Matrix-assisted laser desorption/ionization-time of flight chemotaxonomic analysis supported the gene-based taxonomic placement. We propose the names <i>Bifidobacterium fermentum</i> sp. nov. and <i>Bifidobacterium aquikefiricola</i> sp. nov. for these novel species within the <i>Bifidobacterium</i> genus. The proposed type strain <i>B. fermentum</i> WK012_4_13<sup>T</sup> (= LMG 33104<sup>T</sup> = DSM 116073<sup>T</sup>; GenBank accession number GCF_041080835.1) has a genome size of 2.43 Mbp, with a G+C content of 56.00 mol%. The proposed type strain for <i>B. aquikefiricola</i> WK041_4_12<sup>T</sup> (= LMG 33105<sup>T</sup> = DSM 116074<sup>T</sup>; GenBank accession number GCF_041080795.1) has a genome size of 2.36 Mbp and a G+C content of 53.94 mol%. <i>B. fermentum</i> cells are Gram-positive staining, non-motile, non-spore-forming, fructose-6-phosphate phosphoketolase (F6PPK)-positive, catalase- and oxidase-negative and bacillary club shaped. <i>B. aquikefiricola</i> cells are Gram-positive staining, non-motile, non-spore-forming, F6PPK-positive, catalase- and oxidase-negative and square rod shaped.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11500810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142500561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Erratum: <i>Amycolatopsis nalaikhensis</i> sp. nov. and <i>Amycolatopsis carbonis</i> sp. nov., two novel actinobacteria with antimicrobial activity isolated from a coal mining site in Mongolia.","authors":"Bilguun Oyuntsetseg, Seung Bum Kim","doi":"10.1099/ijsem.0.006541","DOIUrl":"10.1099/ijsem.0.006541","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Notification that new names of prokaryotes, new combinations, and new taxonomic opinions have appeared in volume 74, part 6 of the IJSEM.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006461","DOIUrl":"10.1099/ijsem.0.006461","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142346745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}