International journal of systematic and evolutionary microbiology最新文献

{"title":"<i>Tamlana sedimenti</i> sp. nov. and <i>Catalinimonas locisalis</i> sp. nov., isolated from mangrove swamp and saltern.","authors":"Le Liu, Xiaoxuan Song, Taiyu Lin, Chuanhao Zhang, Zong-Jun Du","doi":"10.1099/ijsem.0.006904","DOIUrl":"10.1099/ijsem.0.006904","url":null,"abstract":"<p><p>Two Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strains, designated SZ-1-7^T and 4WD22^T, were isolated from a mangrove swamp in Shenzhen and a marine solar saltern in Weihai, respectively. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SZ-1-7^T and strain 4WD22^T belong to the phylum <i>Bacteroidota</i>. The percentage of conserved protein and average nucleotide identity values between strain SZ-1-7^T and the genus <i>Tamlana</i> were 56.6-74.0% and 74.7-90.5%, respectively. Strain SZ-1-7^T grew from 15 to 40 °C (optimally at 33 °C), pH 6.0-10.0 (optimally at 7.0-8.0) and in the presence of 0-7% (w/v) NaCl (optimally in 2% NaCl). Strain SZ-1-7^T could produce carotenoid-type pigments. Strain 4WD22^T grew from 20 to 45 °C (optimally at 40 °C), pH 6.0-10.0 (optimally at 8.0) and in the presence of 1-5% (w/v) NaCl (optimally in 2% NaCl). MK-6 was the only respiratory quinone that was compatible with the genus of strain SZ-1-7^T. The strain 4WD22^T contained MK-7 as the predominant menaquinone. The major polar lipids of strain SZ-1-7^T were three kinds of unidentified lipids, five kinds of unidentified aminolipid and phosphatidylethanolamine, and those of strain 4WD22^T were phosphatidylethanolamine, three unidentified aminolipids and five unidentified lipids. The strain SZ-1-7^T was verified to have the potential to degrade polysaccharides in this study. The predatory capability of strain 4WD22^T was tested in this study. The DNA G+C contents of strain SZ-1-7^T and strain 4WD22^T were 36.5 and 41.5 mol%, respectively. Based upon the results presented in this study, strain SZ-1-7^T and strain 4WD22^T represent novel species of the genera <i>Tamlana</i> and <i>Catalinimonas</i>, respectively, for which the names <i>Tamlana sedimenti</i> sp. nov. and <i>Catalinimonas locisalis</i> sp. nov. are proposed with the type strain SZ-1-7^T (=MCCC 1H01428^T=KCTC 102108^T) and strain 4WD22^T (=MCCC 1H01431^T=KCTC 102105^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Radicibacter daui</i>, gen. nov., sp. nov., isolated from tomato rhizosphere soil and proposal of <i>Radicibacteraceae</i> fam. nov. and <i>Radicibacterales</i> ord. nov.","authors":"Joo Hwan Kwon, Lemessa Etana Bultum, Seongmin Lee, Sang-Moo Lee, Minseo Choi, Seon-Woo Lee","doi":"10.1099/ijsem.0.006914","DOIUrl":"10.1099/ijsem.0.006914","url":null,"abstract":"<p><p>A novel bacterial strain UR115^T belonging to the class <i>Alphaproteobacteria</i> was isolated from tomato rhizosphere soil. Strain UR115^T was Gram-stain-negative, motile, facultative anaerobic, catalase positive, oxidase positive and rod shaped. Strain UR115^T showed growth at 15-37 °C, pH 5.5-9.5 and up to 4.0% NaCl. Strain UR115^T exhibited 16S rRNA gene sequence similarities of <90.40% with members of the class <i>Alphaproteobacteria</i>. Based on the phylogenetic analysis inferred from both 16S rRNA gene and whole-genome sequences, strain UR115^T formed a separate and distinct clade, leading to its classification as a member of a novel order within the class <i>Alphaproteobacteria</i>. The genome size and G+C content of strain UR115^T were 5,059,753 bp and 63.9%, respectively. Average amino acid identity values between strain UR115ᵀ and reference genomes ranged from 47.85% to 59.96%, and pairwise Percentage of Conserved Protein values ranged from 4.12% to 46.63%. The major cellular fatty acids of strain UR115^T were C_19 : 0 cyclo <i>ω</i>8<i>c</i>, C_16 : 0 and summed feature 8 (C_18 : 1 <i> ω</i>7<i>c</i> and/or C_18 : 1 <i> ω</i>6<i>c</i>). The polar lipids of strain UR115^T were phosphatidylethanolamine, phosphatidylglycerol, unidentified aminophospholipid, two unidentified aminolipids and two unidentified lipids. The predominant quinone was ubiquinone-10. Based on its distinct phylogenetic position, unique genomic characteristics and chemotaxonomic profiles, we proposed the novel species <i>Radicibacter daui</i> gen. nov., sp. nov., the novel family <i>Radicibacteraceae</i> fam. nov. and the novel order <i>Radicibacterales</i> ord. nov. The type strain is strain UR115^T (=KACC 23674^T=JCM 36738^T).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145053527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Mucilaginibacter metallidurans</i> sp. nov., isolated from a gold and copper mine.","authors":"Yuanping Li, Jiaxin Yuan, Kaixuan Huang, Xiaojun Yang, Panpan Dang, Kangfeng You, Qi Zhang, Jianghua Ye, Christopher Rensing","doi":"10.1099/ijsem.0.006919","DOIUrl":"https://doi.org/10.1099/ijsem.0.006919","url":null,"abstract":"<p><p>In this study, a novel strain P4^T was isolated from a soil sample collected in Longyan, Fujian, PR China. Phylogeny based on 16S rRNA gene sequences suggested that strain P4^T is a new member of the genus <i>Mucilaginibacter</i>. The novel strain showed the highest 16S rRNA gene sequence similarity to <i>Mucilaginibacter rubeus</i> EF23^T (98.4%). The average nucleotide identity and digital DNA-DNA hybridization values between strain P4^T and <i>M. rubeus</i> EF23^T were 93.75% and 66.10%, respectively, and between P4^T and <i>Mucilaginibacter gossypii</i> Gh-67^T were 95.11% and 69.20%, respectively, which were below the prokaryotic species delineation thresholds, suggesting that strain P4^T is a novel species within the genus <i>Mucilaginibacter</i>. The primary fatty acids detected in strain P4^T included summed feature 3 (C_16:1 <i>ω7c</i> and/or C_16:1 <i>ω6c</i>), iso-C_15:0 and C_16:0. The major respiratory quinone in strain P4^T was Menaquinone-7 (MK-7). The high level of phosphatidylethanolamine, along with the presence of multiple other unknown phospholipids like PL1 and PL2, highlights a distinctive membrane composition in strain P4^T. Combined with results stemming from the determination of physical and biochemical characteristics, chemical properties and genome analysis, strain P4^T is proposed to represent a novel species of the genus <i>Mucilaginibacter</i>, for which the name <i>Mucilaginibacter metallidurans</i> sp. nov. is proposed. The type strain is P4^T (=MCCC 1K09773^T=KCTC 102379^T), with the complete genome and 16S rRNA gene sequence deposited in National Center for Biotechnology Information (NCBI) under GenBank accession numbers CP043449.1 and PV576010, respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based reclassification of <i>Parabacteroides chartae</i> as a later heterotypic synonym of <i>Macellibacteroides fermentans</i>.","authors":"Samuel L Miller, Noha H Youssef, Mostafa S Elshahed","doi":"10.1099/ijsem.0.006927","DOIUrl":"https://doi.org/10.1099/ijsem.0.006927","url":null,"abstract":"<p><p>Strain PK034 was isolated from a municipal wastewater treatment plant in Stillwater, OK, USA. A comparison of 16S rRNA gene sequences revealed ≥99.4% sequence similarity to the type strains of <i>Macellibacteroides fermentans</i> (isolated from an up-flow anaerobic filter) and <i>Parabacteroides chartae</i> (isolated from wastewater from a paper mill), suggesting their close relatedness and the possibility of these strains belonging to the same species. The genus <i>Macellibacteroides</i> currently contains a single species, while the genus <i>Parabacteroides</i> currently contains 13 species. Phylogenetic analysis using the 16S rRNA gene, whole-genome phylogenomic analysis and overall genomic-based relatedness indices indicated that PK034 and the type strains of <i>M. fermentans</i> and <i>P. chartae</i> shared average nucleotide identity and digital DNA-DNA hybridization values >95% and >70%, which are the currently accepted thresholds for species-level delineation. Furthermore, such analysis also placed PK034 and the type strains of <i>M. fermentans</i> and <i>P. chartae</i> as a distinct branch within the <i>Tannerellaceae</i> family of the <i>Bacteroidales</i> order, clustering separately from all other species of the genus <i>Parabacteroides</i>. Comparison of phenotypic and physiological traits revealed a high level of shared characteristics and physiological optima between all isolates. Thus, it is proposed that <i>P. chartae</i> NS31-3^T has been erroneously classified as a member of the genus <i>Parabacteroides</i>, that strains PK034, <i>M. fermentans</i> and <i>P. chartae</i> constitute the same species and that <i>P. chartae</i> Tan <i>et al.</i> 2012 should be taken as a later heterotypic synonym of <i>M. fermentans</i> Jabari <i>et al.</i> 2012.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145148938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of <i>Paenibacillus frigoriresistens</i> Ming <i>et al</i>. 2012 as a later heterotypic synonym of <i>Paenibacillus alginolyticus</i> (Nakamura 1987) Shida <i>et al</i>. 1997.","authors":"Baiyuan Li, Ziqi Liang, Dan Chen, Weiwei Mao, Renxia Zhang, Zixun Hu, Jian Gao","doi":"10.1099/ijsem.0.006888","DOIUrl":"10.1099/ijsem.0.006888","url":null,"abstract":"<p><p>In this study, the taxonomic relationship between <i>Paenibacillus frigoriresistens</i> and <i>Paenibacillus alginolyticus</i> was reassessed utilizing a polyphasic taxonomic approach. Molecular identification through 16S rRNA gene sequence analysis demonstrated that <i>P. alginolyticus</i> DSM 5050^T exhibited the highest sequence similarities with <i>Paenibacillus phytorum</i> LMG 31458^T (99.57%), <i>Paenibacillus germinis</i> LMG 31460^T (99.28%), <i>P. frigoriresistens</i> YIM 016^T (99.07%) and <i>Paenibacillus planticolens</i> LMG 31457^T (98.49%). Phylogenetic analyses based on both 16S rRNA gene sequences and whole-genome sequences revealed that <i>P. alginolyticus</i> DSM 5050^T clustered with <i>P. frigoriresistens</i> YIM 016^T, suggesting a close relationship between these two strains. Moreover, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between them were 97.33 and 76.90%, respectively, surpassing the recommended species delineation thresholds of 95-96% for ANI and 70% for dDDH. These results indicated that <i>P. alginolyticus</i> and <i>P. frigoriresistens</i> belonged to the same genomic species. This conclusion was further supported by phenotypic and chemotaxonomic characteristics. According to the data above, we propose that <i>P. frigoriresistens</i> Ming <i>et al</i>. 2012 is a later heterotypic synonym of <i>P. alginolyticus</i> (Nakamura 1987) Shida <i>et al.</i> 1997.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expansion and revision of the genus <i>Xanthobacter</i> and proposal of <i>Roseixanthobacter</i> gen. nov.","authors":"Maximillian P M Soltysiak, Amogh P Jalihal, Caroline E Christophersen, Audrey L H Ory, Andrew D Lee, Jessica Boulton, Michael Springer","doi":"10.1099/ijsem.0.006909","DOIUrl":"https://doi.org/10.1099/ijsem.0.006909","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The nitrogen-fixing, chemolithoautotrophic genus &lt;i&gt;Xanthobacter&lt;/i&gt; is found across numerous diverse environments worldwide and is an important member of many ecosystems. These species serve as model systems for their metabolic properties and have industrial applications in bioremediation and sustainable protein, food and fertilizer production. Despite their abundance and utility, the majority of &lt;i&gt;Xanthobacter&lt;/i&gt; strains are without a genome sequence, and only eight validly published species are known to date. To expand our understanding of the diversity and evolutionary history of the genus, we sequenced the genomes of 37 repository strains and 26 novel environmental strains we isolated. After performing comparative phylogenomic analyses, we expand and revise the genus &lt;i&gt;Xanthobacter&lt;/i&gt; and propose the novel genus &lt;i&gt;Roseixanthobacter&lt;/i&gt; gen. nov. For &lt;i&gt;Xanthobacter&lt;/i&gt;, we describe 9 novel species (bringing the total to 17): &lt;i&gt;Xanthobacter albus&lt;/i&gt; sp. nov. (V0C-6&lt;sup&gt;T&lt;/sup&gt;=DSM 117699&lt;sup&gt;T&lt;/sup&gt;=ATCC TSD-450&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter cornucopiae&lt;/i&gt; sp. nov. (V4C-4&lt;sup&gt;T&lt;/sup&gt;=DSM 117704&lt;sup&gt;T&lt;/sup&gt;=ATCC TSD-451&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter lutulentifluminis&lt;/i&gt; sp. nov. (V3C-3&lt;sup&gt;T&lt;/sup&gt;=DSM 117706&lt;sup&gt;T&lt;/sup&gt;=ATCC TSD-452&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter nonsaccharivorans&lt;/i&gt; sp. nov. (14g&lt;sup&gt;T&lt;/sup&gt;=DSM 431&lt;sup&gt;T&lt;/sup&gt;=JCM 1201&lt;sup&gt;T&lt;/sup&gt;=CIP 105432&lt;sup&gt;T&lt;/sup&gt;=NCIMB 10811&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter pseudotagetidis&lt;/i&gt; sp. nov. (KA&lt;sup&gt;T&lt;/sup&gt;=DSM 11602&lt;sup&gt;T&lt;/sup&gt;=KCTC 8467&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter sediminis&lt;/i&gt; sp. nov. (V8C-5&lt;sup&gt;T&lt;/sup&gt;=DSM 117708&lt;sup&gt;T&lt;/sup&gt;=ATCC TSD-453&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter variabilis&lt;/i&gt; sp. nov. (V4C-8&lt;sup&gt;T&lt;/sup&gt;=DSM 117713&lt;sup&gt;T&lt;/sup&gt;=ATCC TSD-454&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Xanthobacter versatilis&lt;/i&gt; sp. nov. (Py2&lt;sup&gt;T&lt;/sup&gt;=DSM 118458&lt;sup&gt;T&lt;/sup&gt;=ATCC BAA-1158&lt;sup&gt;T&lt;/sup&gt;) and &lt;i&gt;Xanthobacter wiegelii&lt;/i&gt; sp. nov. (RH 10&lt;sup&gt;T&lt;/sup&gt;=DSM 597&lt;sup&gt;T&lt;/sup&gt;=JCM 7864&lt;sup&gt;T&lt;/sup&gt;=CIP 105437&lt;sup&gt;T&lt;/sup&gt;). For &lt;i&gt;Roseixanthobacter&lt;/i&gt; gen. nov., we describe five novel species formerly classified as &lt;i&gt;Xanthobacter&lt;/i&gt;: &lt;i&gt;Roseixanthobacter finlandensis&lt;/i&gt; sp. nov. (VTT E-85241&lt;sup&gt;T&lt;/sup&gt;=BIO GISA 20&lt;sup&gt;T&lt;/sup&gt;=DSM 117884&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Roseixanthobacter glucoisosaccharinicivorans&lt;/i&gt; sp. nov. (VTT E-85242&lt;sup&gt;T&lt;/sup&gt;=BIO GISA 21&lt;sup&gt;T&lt;/sup&gt;=DSM 117885&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Roseixanthobacter liquoris&lt;/i&gt; sp. nov. (VTT E-85238&lt;sup&gt;T&lt;/sup&gt;=BIO GISA 16&lt;sup&gt;T&lt;/sup&gt;=DSM 117882&lt;sup&gt;T&lt;/sup&gt;), &lt;i&gt;Roseixanthobacter pseudopolyaromaticivorans&lt;/i&gt; sp. nov. (VTT E-85240&lt;sup&gt;T&lt;/sup&gt;=BIO GISA 19&lt;sup&gt;T&lt;/sup&gt;=DSM 117883&lt;sup&gt;T&lt;/sup&gt;) and &lt;i&gt;Roseixanthobacter psychrophilus&lt;/i&gt; sp. nov. (W30&lt;sup&gt;T&lt;/sup&gt;=DSM 24535&lt;sup&gt;T&lt;/sup&gt;=KCTC 8466&lt;sup&gt;T&lt;/sup&gt;). We characterized the phenotypic properties of these type strains, including temperature, salinity and pH ranges, carbon substrate utilization, motility, slime production, autotrophic growth and enzymatic activities. We discovered a more diverse range of phenotypes ac","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EzBioCloud 16S rRNA Gene Sequence Formatter: a Python-based sequence formatting tool for systematic microbiology.","authors":"Rashidin Abdugheni, Wen-Jun Li","doi":"10.1099/ijsem.0.006890","DOIUrl":"https://doi.org/10.1099/ijsem.0.006890","url":null,"abstract":"<p><p>EzBioCloud is one of the practical reference databases and analytical platforms for systematic microbiology research. The EzBioCloud database provides convenient services in this regard, especially for performing sequence analysis using the 16S rRNA genes. However, '.fasta' files of 16S rRNA sequences obtained after the alignment in EzBioCloud need manual formatting for further analysis and phylogenetic tree construction, which is labourious and time-consuming. To address this issue, we have developed a Python-based tool, EzBioCloud 16S rRNA Gene Sequence Formatter (version 1.0), designed to assist in sequence formatting. Here, we report the development and application of the tool and present this tool publicly to support the researchers in the relevant field.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposal of <i>Streptomyces janthinus</i> (Artamonova and Krassilnikov 1960) Pridham 1970 (Approved Lists 1980) as a later heterotypic synonym of <i>Streptomyces violaceus</i> (Rossi Doria 1891) Waksman 1953 (Approved Lists 1980) and an emended description of <i>Streptomyces violaceus</i>.","authors":"Kaiqin Li, Ying Qian, Bin Li, Wei Liang","doi":"10.1099/ijsem.0.006892","DOIUrl":"10.1099/ijsem.0.006892","url":null,"abstract":"<p><p>The taxonomic relationship between <i>Streptomyces janthinus</i> and <i>Streptomyces violaceus</i> was re-evaluated using comparative genome analysis. The 16S rRNA gene sequence analysis indicated that the type strains of <i>S. janthinus</i> and <i>S. violaceus</i> shared 100% sequence similarity. Phylogenetic trees based on 16S rRNA gene sequences indicated that <i>S. janthinus</i> JCM 4387^T was clustered together with <i>S. violaceus</i> CGMCC 4.1456^T. These results suggested that <i>S. janthinus</i> JCM 4387^T was most closely related to <i>S. violaceus</i> CGMCC 4.1456^T. The phylogenomic tree also supported this result. The average nt identity (ANI) and dDDH values between these two strains were 97.89% and 81.0%, respectively, well more than the 96.7% ANI and 70% dDDH threshold values for <i>Streptomyces</i> species delineation, confirming that <i>S. janthinus</i> JCM 4387^T and <i>S. violaceus</i> CGMCC 4.1456^T belong to the same genomic species. In addition, a comprehensive comparison of phenotypic, chemotaxonomic and genomic characteristics also supported this conclusion. According to all these results and Rule 42 of the Bacteriological Code, we propose <i>S. janthinus</i> (Artamonova and Krassilnikov 1960) Pridham 1970 (Approved Lists 1980) as a later heterotypic synonym of <i>S. violaceus</i> (Rossi Doria 1891) Waksman 1953 (Approved Lists 1980).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptomyces mayonensis</i> sp. nov., isolated from the volcanic soils of Mt. Mayon, Philippines.","authors":"Gerald M Aguilar, Kristel Mae P Oliveros, Albert Remus R Rosana, Rina B Opulencia, Asuncion K Raymundo","doi":"10.1099/ijsem.0.006900","DOIUrl":"https://doi.org/10.1099/ijsem.0.006900","url":null,"abstract":"<p><p>A novel actinobacterium, designated as strain A1-08^T, was isolated from the volcanic soils of Mt. Mayon, Philippines. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain A1-08^T belonged to the genus <i>Streptomyces</i> with sequence similarity with <i>Streptomyces djakartensis</i> NBRC 15409^T (99.02%), <i>Streptomyces tuirus</i> NBRC 15617^T (98.88%) and <i>Streptomyces mutabilis</i> NBRC 12800^T (98.81%). Polyphasic characterization demonstrated distinct morphological, cultural, physiological and biochemical features with unique lipid and fatty acid profiles and respiratory quinones. The major phospholipids of strain A1-08^T are diphosphatidylglycerol, phosphatidylethanolamine, glycophospholipid with mannose and/or galactose and unidentified lipids such as aminolipid and phospholipid. The major cellular fatty acids were anteiso-C_15 : 0, C_16 : 0, anteiso-C_17 : 0, iso-C_16 : 0, iso-C_15 : 0 and C_16 : 1  <i>ω</i>7c. The respiratory quinones of the strain include MK-9 (H6), MK-9 (H8), MK-10 (H6) and MK-10 (H8). Multilocus analysis using the concatenated sequences of <i>atpD</i>, <i>gyrB</i>, <i>recA</i>, <i>rpoB</i> and <i>trpB</i> showed that the strain formed a distinct branch in the genus <i>Streptomyces.</i> Potential genes for environmental stress response and adaptation were identified in the genome of strain A1-08^T and its closest relative strains. Whole-genome sequencing confirmed A1-08^T as a novel species within <i>Streptomyces</i> with 57.80% digital DNA-DNA hybridization and 93.03% average nt identity value with the closest type strain <i>Streptomyces olivaceus</i> NRRL B-3009^T. The name <i>Streptomyces mayonensis</i> sp. nov., derived from the Mayon Volcano in the Philippines, is hereby proposed (type strain A1-08^T =JCM 36345, =DSM 118395).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12401495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144954108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Carbonatibacter coralli</i> gen. nov., sp. nov., a carbonate-producing and vitamin B₁₂-sharing bacterium isolated from coral <i>Porites lutea</i>, reclassification of <i>Kiloniellaceae</i> and proposal of <i>Aestuariispiraceae</i> fam. nov.","authors":"Siyu Hu, Kun Lu, Chenyan Wang, Mi Li, Jinsheng Huang, Guanghua Wang","doi":"10.1099/ijsem.0.006928","DOIUrl":"https://doi.org/10.1099/ijsem.0.006928","url":null,"abstract":"<p><p>A Gram-stain-negative, non-spore-forming, carbonate-producing, vitamin B₁₂-sharing, aerobic rod, designated strain GXU_MW_B19^T, was isolated from coral <i>Porites lutea</i>. Cells grew at 20-40 °C, pH 6.5-10 and no more than 5% NaCl (w/v). Global alignment based on 16S rRNA gene sequence indicated that the new isolate is most close to members of the genus <i>Kiloniella</i> with identities of 92.9-94.1%, while sharing no more than 91.7% similarity with other type strains. The maximum average nucleotide identity and average amino acid identity to closely related species were 69.5 and 62.8%, respectively. Phylogenetic analysis based on both 16S rRNA gene sequences and the whole-genome sequences indicated that strain GXU_MW_B19^T forms a stable cluster with genus <i>Kiloniella</i> in the order <i>Rhodospirillales</i>. The genome G+C content was 54.5 mol%. The dominant cellular fatty acids (≥10%) included iso-C_10 : 02OH, C_12 : 1, C_14 : 1 ω5c and iso-C_17 : 03OH. The major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, unidentified ninhydrin-positive lipid and three unidentified lipids. Alpha-1,6-glucosidase, alkaline phosphatase, isochorismate-related enzymes and P2-like prophage-related proteins were encoded in the new isolate, all of which were absent in closely related species. Based on these distinct phylogenetic, genomic and chemotaxonomic characteristics, the new isolate is suggested to represent a new species in a new genus, for which the name <i>Carbonatibacter coralli</i> gen. nov., sp. nov. is proposed. The type strain is GXU_MW_B19^T (=KCTC 8092^T=MCCC 1K08794^T). Finally, phylogenetic analyses led to the reclassification of the <i>Kiloniellaceae</i> and the proposal of ‌<i>Aestuariispiraceae‌</i> fam. nov.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 9","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}