Ryan Michael Thompson, Edward M Fox, Georgios Koutsidis, Maria Del Carmen Montero-Calasanz
{"title":"<i>Amycolatopsis ponsaeliensis</i> sp. nov., a novel endophytic actinobacterium isolated from the root nodules of <i>Alnus glutinosa</i>.","authors":"Ryan Michael Thompson, Edward M Fox, Georgios Koutsidis, Maria Del Carmen Montero-Calasanz","doi":"10.1099/ijsem.0.006810","DOIUrl":"https://doi.org/10.1099/ijsem.0.006810","url":null,"abstract":"<p><p>The root nodules of <i>Alnus glutinosa</i> remain a relatively understudied niche, with poorly described associated microbial communities. In this study, the isolate RTGN1<sup>T</sup> was recovered from root nodules collected from Gateshead, UK, and was identified as belonging to <i>Amycolatopsis</i> based on 16S rRNA gene similarity and phylogenomic placement. This isolate was polyphasically characterized, displaying the ability to grow between 12 and 28 °C and pH 6 and 8 and exhibiting the genes necessary to produce the polar lipids phosphatidylethanolamine and phosphatidylglycerophosphate, alongside DL-type peptidoglycan, which are diagnostic of <i>Amycolatopsis</i>. Overall genomic relatedness index values were below the cut-off value for delineating a novel species. As such, it is proposed that RTGN1<sup>T</sup> be recognized as the type strain (=CECT 30870<sup>T</sup>=CABI 507287<sup>T</sup>) of <i>Amycolatopsis ponsaeliensis</i> sp. nov. The RTGN1<sup>T</sup> isolate was screened using <i>in silico</i> and <i>in vitro</i> methods and was found to possess a number of genes and pathways related to secondary metabolite production and plant growth promotion. Such genes may serve as an avenue of future study regarding biotechnological potential and use as a bioinoculant to increase phytoremediation efficiency.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Chryseobacterium gossypii</i> sp. nov., a bacterium isolated from long-term continuous cropping cotton fields.","authors":"Mila Mulati, Delong Kong, Lili Chai, Yuanyang Yi, Zhidong Zhang, Wei Zhang","doi":"10.1099/ijsem.0.006817","DOIUrl":"10.1099/ijsem.0.006817","url":null,"abstract":"<p><p>A novel bacterium, designated 1RM2<sup>T</sup>, was isolated from Xinjiang Province, north-west PR China. This strain could grow under conditions of 20-45 °C, pH 5.0-10.0 and 0-10% (w/v) NaCl. The species with the highest similarity of 16S rRNA gene sequences to strain 1RM2<sup>T</sup> were strain Chryseobacterium bernardetii NCTC 13530<sup>T</sup> (97.1%) and <i>Chryseobacterium daecheongense</i> DSM 15235<sup>T</sup> (96.9%). The draft genome sequence G+C content of strain 1RM2<sup>T</sup> was 39.5 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain 1RM2<sup>T</sup> and the two closest neighbours were 78.6%, 77.6% and 21.8%, 21.2%, respectively. The main fatty acids of strain 1RM2<sup>T</sup> were iso-C<sub>15:0</sub>, iso-C<sub>17:0</sub> 3-OH, summed feature 3 (C<sub>16:1</sub> <i> ω</i>6<i>c</i> and/or C<sub>16:1</sub> <i> ω</i>7<i>c</i>) and summed feature 9 (C<sub>16:0</sub> 10-methyl and/or iso-C<sub>17:1</sub> <i> ω</i>9<i>c</i>). The main isoprenoid quinone was menaquinone-6 and polar lipids were phosphatidylethanolamine, unidentified amino phospholipids and unidentified lipids. Based on phenotypic characteristics and genotype analysis, strain 1RM2<sup>T</sup> is a new species of the genus <i>Chryseobacterium</i> and is proposed to be named <i>Chryseobacterium gossypii</i> sp. nov. (=GDMCC 1.4437<sup>T</sup>=KCTC 102275<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Comamonas trifloxystrobinivorans</i> sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.","authors":"Qian Zhu, Kangning Wei, Kaihua Pan, Qian Li, Weihao Zhu, Mingliang Zhang, Qing Hong","doi":"10.1099/ijsem.0.006796","DOIUrl":"10.1099/ijsem.0.006796","url":null,"abstract":"<p><p>A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3<sup>T</sup>, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3<sup>T</sup> could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3<sup>T</sup>; it exhibited low similarity (<i><</i>31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3<sup>T</sup> was most closely related to strain <i>Comamonas odontotermitis</i> Dant 3-8<sup>T</sup>. The 16S rRNA gene similarity between strain J-3<sup>T</sup> and <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3<sup>T</sup> and strain <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> were 81.7% and 25.4%, respectively. The major fatty acids were C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub> <i> ω7</i>c and/or C<sub>16:1</sub> <i> ω6</i>c) and summed feature 8 (C<sub>18 : 1</sub> <i> ω7</i>c and/or C<sub>18 : 1</sub> <i> ω6</i>c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3<sup>T</sup> was considered to represent a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas trifloxystrobinivorans</i> sp. nov. is proposed with strain J-3<sup>T</sup> (=KCTC 8781<sup>T</sup>=MCCC 1K09559<sup>T</sup>) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Scott V Nguyen, Vicktor H Escobar, Shahin S Ali, Nikhita P Puthuveetil, Joseph R Petrone, Jade L Kirkland, Kaitlyn Gaffney, Corina L Tabron, Noah Wax, James Duncan, Stephen King, Robert Marlow, Amy L Reese, David A Yarmosh, Hannah H McConnell, Ana S Fernandes, John Bagnoli, Briana Benton, Jonathan L Jacobs
{"title":"Reclassification of atypical <i>Moraxella catarrhalis</i> ATCC 23246 as <i>Moraxella veridica</i> sp. nov.","authors":"Scott V Nguyen, Vicktor H Escobar, Shahin S Ali, Nikhita P Puthuveetil, Joseph R Petrone, Jade L Kirkland, Kaitlyn Gaffney, Corina L Tabron, Noah Wax, James Duncan, Stephen King, Robert Marlow, Amy L Reese, David A Yarmosh, Hannah H McConnell, Ana S Fernandes, John Bagnoli, Briana Benton, Jonathan L Jacobs","doi":"10.1099/ijsem.0.006797","DOIUrl":"10.1099/ijsem.0.006797","url":null,"abstract":"<p><p>Whole-genome sequencing of cultures at the American Type Culture Collection (ATCC<sup>®</sup>) is ongoing, with reference-quality genome sequences for our microbial strains added to the ATCC<sup>®</sup> Genome Portal on a quarterly basis. Following genome assembly, authentication and taxonomy verification are needed for taxonomic updates based on the circumscription of genomic metrics for a species. <i>Moraxella</i> sp. ATCC 23246<sup>T</sup> was originally identified as an atypical <i>Moraxella catarrhalis</i>; however, an analysis of the complete and closed genome of this strain indicates that it represents a novel species within the <i>Moraxella</i> genus. We propose the name of <i>Moraxella veridica</i> sp. nov. for this long-mischaracterized strain as whole-genome sequencing was used to uncover the truth of this strain's identity. The type strain is ATCC 23246<sup>T</sup> (=NCTC 4103<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt
{"title":"Description of <i>Labrys sedimenti</i> sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of <i>Labrys portucalensis</i> as a later heterotypic synonym of <i>Labrys neptuniae</i>.","authors":"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt","doi":"10.1099/ijsem.0.006778","DOIUrl":"https://doi.org/10.1099/ijsem.0.006778","url":null,"abstract":"<p><p>A Gram-stain-negative strain, designated as Zidic-5<sup>T</sup>, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5<sup>T</sup> belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578<sup>T</sup> (99.13%), followed by <i>Labrys portucalensis</i> F11<sup>T</sup> (99.06%), <i>Labrys methylaminiphilus</i> JLW10<sup>T</sup> (98.58%) and <i>Labrys okinawensis</i> DSM 18385<sup>T</sup> (98.32%). The draft genome sequence of strain Zidic-5<sup>T</sup> is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5<sup>T</sup> and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5<sup>T</sup> is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5<sup>T</sup> were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5<sup>T</sup> were C<sub>18 : 1</sub> ω7c, C<sub>16 : 0</sub> and C<sub>19 : 0</sub> cyclo ω7c. The major ubiquinone of strain Zidic-5<sup>T</sup> was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5<sup>T</sup> represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5<sup>T</sup> (=LMG 33565<sup>T</sup>=NCAIM B.02686<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Vreelandella arctica</i> sp. nov. and <i>Vreelandella indica</i> sp. nov., isolated from the Arctic Ocean and the Southwest Indian Ocean sediment.","authors":"Yuwei Weng, Jingyu Xia, Xuying Zhu, Wanpeng Wang","doi":"10.1099/ijsem.0.006791","DOIUrl":"10.1099/ijsem.0.006791","url":null,"abstract":"<p><p>Four Gram-stain-negative bacterial strains which can metabolize alkanes and polycyclic aromatic hydrocarbons were isolated. Strains R06ZXJ2<sup>T</sup> and R09ZXJ6 were isolated from sediments collected in the Arctic Ocean. Strains DY715-9<sup>T</sup> and DY716-13-1 were isolated from surface sediment samples collected by the deep-sea TV grab in the southwestern Indian Ocean. Phylogenomic analyses based on 16S rRNA gene sequences, average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) revealed that the strains formed two distinct lineages within the genus <i>Vreelandella</i>. Their closest relative was <i>Vreelandella maris</i> QX-1<sup>T</sup>, with ANI values of 94.35% (R06ZXJ2<sup>T</sup>) and 95.54% (DY715-9<sup>T</sup>) and dDDH values of 57.00% and 61.80%, respectively. The pairwise ANI (95.47%) and dDDH (60.70%) between the two novel strains further supported their status as separate species. Phenotypic and chemotaxonomic analyses distinguished them from known <i>Vreelandella</i> species through differences in colony morphology, hydrocarbon degradation profiles and polar lipid compositions. The strains grew optimally at 28 °C and pH 7.0 and can degrade alkanes and polycyclic aromatic hydrocarbons. Based on these results, we propose two novel species: <i>Vreelandella arctica</i> sp. nov. (type strain R06ZXJ2<sup>T</sup>=MCCC 1A08467<sup>T</sup>=KCTC 92631<sup>T</sup>) and <i>Vreelandella indica</i> sp. nov. (type strain DY715-9<sup>T</sup>=MCCC 1A12469<sup>T</sup>=KCTC 92628<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Validation List no. 223. Valid publication of new names and new combinations effectively published outside the <i>IJSEM</i>.","authors":"Aharon Oren, Markus Göker","doi":"10.1099/ijsem.0.006699","DOIUrl":"https://doi.org/10.1099/ijsem.0.006699","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wei Shu, Christian Rückert-Reed, Olexandr Gromyko, Stepan Tistechok, Jörn Kalinowski, Andriy Luzhetskyy, Christoph Wittmann
{"title":"Description of <i>Streptomyces explomaris</i> sp. nov., isolated from the coastal soil rhizosphere of <i>Juniperus excelsa</i> and reclassification of <i>Streptomyces libani</i> as a later heterotypic synonym of <i>Streptomyces nigrescens</i>.","authors":"Wei Shu, Christian Rückert-Reed, Olexandr Gromyko, Stepan Tistechok, Jörn Kalinowski, Andriy Luzhetskyy, Christoph Wittmann","doi":"10.1099/ijsem.0.006711","DOIUrl":"10.1099/ijsem.0.006711","url":null,"abstract":"<p><p>The strain Je 1-4<sup>T</sup> was isolated from the coastal rhizosphere soil of <i>Juniperus excelsa</i> M. Bieb. (Crimean Peninsula). Phylogenetic studies based on the 16S rRNA gene sequence revealed that Je 1-4<sup>T</sup> is phylogenetically close to <i>Streptomyces libani</i> subsp. <i>libani</i> NBRC 13452<sup>T</sup> (JCM 4322) and <i>Streptomyces nigrescens</i> NBRC 12894<sup>T</sup> (DSM 40276) with sequence similarities of 99.86 to 99.93%. The genome of strain Je 1-4<sup>T</sup> consisted of a linear chromosome with a size of 8.9 Mbp and a G+C content of 70.9mol%. Digital DNA-DNA hybridization (dDDH) analysis showed that Je 1-4<sup>T</sup> is distinct from both <i>S. libani</i> subsp. <i>libani</i> and <i>S. nigrescens</i> (dDDH values of 66.6% and 66.7 %, respectively), while the latter two strains likely represent the same species (dDDH value of 92.0%). The predominant fatty acids in the strains were iso-C<sub>16:0</sub>, anteiso-C<sub>17:0</sub> and anteiso-C<sub>15:0</sub>, and the major menaquinones were MK9 H6 and MK9 H8. Based on these genomic and phenotypic data, strain Je 1-4<sup>T</sup> represents a novel species of <i>Streptomyces</i>, for which the name <i>Streptomyces explomaris</i> sp. nov. is proposed. The type strain is Je 1-4<sup>T</sup> (=DSM 117375<sup>T</sup>=LMG 33490<sup>T</sup>). Additionally, we propose that <i>S. libani</i> subsp. <i>libani</i> Baldacci and Grein 1966 (Approved Lists 1980) is a later heterotypic synonym of <i>S. nigrescens</i> (Sveshnikova 1957) Pridham et al. 1958 (Approved Lists 1980).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12163732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144186985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore
{"title":"<i>Streptococcus hepaticus</i> sp. nov. isolated from the liver of domestic pigs (<i>Sus scrofa domesticus</i>).","authors":"Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore","doi":"10.1099/ijsem.0.006776","DOIUrl":"https://doi.org/10.1099/ijsem.0.006776","url":null,"abstract":"<p><p>A Gram-positive coccus-shaped bacterium, which could not be identified by classical determinative bacteriology approaches, was isolated from the liver of two pigs in the United Kingdom. Initial testing based on cellular morphology and biochemical characteristics tentatively assigned the isolates to the genus <i>Streptococcus</i> but did not match any previously described species. The analysis of the 16S rRNA sequence determined that the isolates were most closely related to <i>Streptococcus gallinaceus</i> (98.6% identity). Analysis of three further housekeeping genes frequently applied in streptococcal taxonomy, <i>groEL</i>, <i>sodA</i> and <i>rpoB</i>, and a comparison against available type strain sequences confirmed that the isolates were most similar to <i>S. gallinaceus</i> in all cases (84.3%, 86.9% and 90.2% identity, respectively). The comparison of the average nucleotide identity (ANI) and <i>in silico</i> DNA-DNA hybridization values demonstrated that the novel species was distinct from other streptococcal species. Pairwise ANI values revealed that the two studied strains shared a pairwise ANI of 99.25% but were clearly distinct from previously described <i>Streptococcus</i> species (ANI ≤81.1% - best match <i>S. gallinaceus</i>). The taxonomic analysis described confirmed that the two strains represent a novel <i>Streptococcus</i> species for which the name <i>Streptococcus hepaticus</i> sp. nov. is suggested, with strain 20-1249<sup>T</sup> (=NCTC 15092<sup>T</sup>=LMG 33498<sup>T</sup>) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charlotte Peeters, Stephanie Steyaert, Matan Shelomi, Anneleen D Wieme, Eliza Depoorter, Evelien De Canck, Kurt Houf, Peter Vandamme
{"title":"<i>Imbroritus primus</i> gen. nov., sp. nov., a facultatively autotrophic bacterium from environmental water samples.","authors":"Charlotte Peeters, Stephanie Steyaert, Matan Shelomi, Anneleen D Wieme, Eliza Depoorter, Evelien De Canck, Kurt Houf, Peter Vandamme","doi":"10.1099/ijsem.0.006781","DOIUrl":"https://doi.org/10.1099/ijsem.0.006781","url":null,"abstract":"<p><p>A Gram-stain-negative coccobacillus, LMG 32992<sup>T</sup>, was isolated from water that had collected in a tyre in Pingtung, Donggang Township, Taiwan. Upon preliminary 16S rRNA gene sequence analysis, it was most closely related to members of the genus <i>Ralstonia</i> (16S rRNA gene sequence similarities of 96.7-97.5%). The present study aimed to elucidate its taxonomic position and to propose a formal classification. To this end, the complete genome sequence was determined, and taxonomic, phylogenomic, metabolic and physiological analyses were performed. Comparative genomic analyses demonstrated that strain LMG 32992<sup>T</sup> and another unclassified strain, <i>Burkholderiaceae</i> bacterium PBA, which was isolated earlier from textile wastewater in Malaysia, represented a single novel species within a novel genus of the family <i>Burkholderiaceae</i>. The G+C content of the LMG 32992<sup>T</sup> genomic DNA was 63.77 mol%. Genomic analyses and growth tests demonstrated that LMG 32992<sup>T</sup> had an asaccharolytic metabolism but that it was well-equipped to synthetize, if necessary autotrophically, and transform all required carbohydrates and that it used the Krebs and related cycles to generate reductive power for a heterotrophic energy metabolism. We propose the name <i>Imbroritus primus</i> gen. nov., sp. nov. with strain LMG 32992<sup>T</sup> (=CIP 112179<sup>T</sup>=BCRC 81361<sup>T</sup>=A30B1<sup>T</sup>) as the type strain, for this novel taxon.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}