{"title":"编码一种新的酯酶,负责降解三氯虫酯酶。","authors":"Qian Zhu, Kangning Wei, Kaihua Pan, Qian Li, Weihao Zhu, Mingliang Zhang, Qing Hong","doi":"10.1099/ijsem.0.006796","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3<sup>T</sup>, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3<sup>T</sup> could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3<sup>T</sup>; it exhibited low similarity (<i><</i>31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3<sup>T</sup> was most closely related to strain <i>Comamonas odontotermitis</i> Dant 3-8<sup>T</sup>. The 16S rRNA gene similarity between strain J-3<sup>T</sup> and <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3<sup>T</sup> and strain <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> were 81.7% and 25.4%, respectively. The major fatty acids were C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub> <i> ω7</i>c and/or C<sub>16:1</sub> <i> ω6</i>c) and summed feature 8 (C<sub>18 : 1</sub> <i> ω7</i>c and/or C<sub>18 : 1</sub> <i> ω6</i>c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3<sup>T</sup> was considered to represent a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas trifloxystrobinivorans</i> sp. nov. is proposed with strain J-3<sup>T</sup> (=KCTC 8781<sup>T</sup>=MCCC 1K09559<sup>T</sup>) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Comamonas trifloxystrobinivorans</i> sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.\",\"authors\":\"Qian Zhu, Kangning Wei, Kaihua Pan, Qian Li, Weihao Zhu, Mingliang Zhang, Qing Hong\",\"doi\":\"10.1099/ijsem.0.006796\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3<sup>T</sup>, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3<sup>T</sup> could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3<sup>T</sup>; it exhibited low similarity (<i><</i>31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3<sup>T</sup> was most closely related to strain <i>Comamonas odontotermitis</i> Dant 3-8<sup>T</sup>. The 16S rRNA gene similarity between strain J-3<sup>T</sup> and <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3<sup>T</sup> and strain <i>C. odontotermitis</i> Dant 3-8<sup>T</sup> were 81.7% and 25.4%, respectively. The major fatty acids were C<sub>16:0</sub>, summed feature 3 (C<sub>16:1</sub> <i> ω7</i>c and/or C<sub>16:1</sub> <i> ω6</i>c) and summed feature 8 (C<sub>18 : 1</sub> <i> ω7</i>c and/or C<sub>18 : 1</sub> <i> ω6</i>c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3<sup>T</sup> was considered to represent a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas trifloxystrobinivorans</i> sp. nov. is proposed with strain J-3<sup>T</sup> (=KCTC 8781<sup>T</sup>=MCCC 1K09559<sup>T</sup>) as the type strain.</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 5\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006796\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006796","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Comamonas trifloxystrobinivorans sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.
A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3T, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3T could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3T; it exhibited low similarity (<31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3T was most closely related to strain Comamonas odontotermitis Dant 3-8T. The 16S rRNA gene similarity between strain J-3T and C. odontotermitis Dant 3-8T was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3T and strain C. odontotermitis Dant 3-8T were 81.7% and 25.4%, respectively. The major fatty acids were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3T was considered to represent a novel species within the genus Comamonas, for which the name Comamonas trifloxystrobinivorans sp. nov. is proposed with strain J-3T (=KCTC 8781T=MCCC 1K09559T) as the type strain.
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.