编码一种新的酯酶,负责降解三氯虫酯酶。

IF 2 3区 生物学 Q4 MICROBIOLOGY
Qian Zhu, Kangning Wei, Kaihua Pan, Qian Li, Weihao Zhu, Mingliang Zhang, Qing Hong
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引用次数: 0

摘要

从安徽省淮北市南湖湿地公园的活性污泥中分离到一株革兰氏阴性、能降解三氯虫酯的严格好氧运动菌J-3T。在0-1.0% NaCl (w/v;在15-35°C(最适:30°C)和pH 5.0-8.0(最适:pH 7.5)条件下。菌株J-3T在培养3 d内对75.0µM三氟虫胺的降解率为59.9%。在菌株J-3T中鉴定出一种新的酯酶,负责将三氯虫酯酶水解为三氯虫酯酸。它与先前报道的三氯酯酶具有较低的相似性(31%),表明其新颖性。基于16S rRNA基因序列和基因组的系统发育分析表明,菌株J-3T与菌株Comamonas odontotermitis Dant 3-8T亲缘关系最密切。菌株J-3T与C. odontotermitis Dant 3-8T的16S rRNA基因相似性为97.7%。J-3T和C. odontotermitis Dant 3-8T的平均核苷酸同源性和数字DNA-DNA杂交值分别为81.7%和25.4%。主要脂肪酸为C16:0、总特征3 (C16:1 ω7c和/或C16:1 ω6c)和总特征8 (C18:1 ω7c和/或C18:1 ω6c)。主要极性脂质为二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。主要呼吸醌为Q-8。基于表型、化学分类、系统发育和基因组学分析,认为菌株J-3T代表了Comamonas属中的一个新种,并以菌株J-3T (=KCTC 8781T=MCCC 1K09559T)为型菌株命名为Comamonas trifloxystrobinivorans sp. nov。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comamonas trifloxystrobinivorans sp. nov., encoding a novel esterase responsible for the degradation of trifloxystrobin.

A Gram-stain-negative, strictly aerobic and motile bacterium capable of degrading trifloxystrobin, designated strain J-3T, was isolated from activated sludge from Nanhu Wetland Park in Huaibei City, Anhui Province, PR China. Growth was observed at 0-1.0% NaCl (w/v; optimum: 0.5%) at 15-35 °C (optimum: 30 °C) and pH 5.0-8.0 (optimum: pH 7.5). Strain J-3T could degrade 59.9% of 75.0 µM trifloxystrobin within 3 days of incubation. A novel esterase responsible for hydrolysing trifloxystrobin to trifloxystrobin acid, StrE, was identified in strain J-3T; it exhibited low similarity (<31%) with previously reported trifloxystrobin esterases, indicating its novelty. Phylogenetic analysis based on 16S rRNA gene sequences and genomes indicated that strain J-3T was most closely related to strain Comamonas odontotermitis Dant 3-8T. The 16S rRNA gene similarity between strain J-3T and C. odontotermitis Dant 3-8T was 97.7%. The average nucleotide identity and digital DNA-DNA hybridization values between strain J-3T and strain C. odontotermitis Dant 3-8T were 81.7% and 25.4%, respectively. The major fatty acids were C16:0, summed feature 3 (C16:1  ω7c and/or C16:1  ω6c) and summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. Based on the phenotypic, chemotaxonomic, phylogenetic and genomic analysis, strain J-3T was considered to represent a novel species within the genus Comamonas, for which the name Comamonas trifloxystrobinivorans sp. nov. is proposed with strain J-3T (=KCTC 8781T=MCCC 1K09559T) as the type strain.

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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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