Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt
{"title":"从双氯芬酸降解富集培养物中分离的Labrys sedimenti sp. nov.的描述,以及基于基因组的Labrys portucalensis作为Labrys neptuniae后异型同义词的重新分类。","authors":"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt","doi":"10.1099/ijsem.0.006778","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative strain, designated as Zidic-5<sup>T</sup>, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5<sup>T</sup> belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578<sup>T</sup> (99.13%), followed by <i>Labrys portucalensis</i> F11<sup>T</sup> (99.06%), <i>Labrys methylaminiphilus</i> JLW10<sup>T</sup> (98.58%) and <i>Labrys okinawensis</i> DSM 18385<sup>T</sup> (98.32%). The draft genome sequence of strain Zidic-5<sup>T</sup> is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5<sup>T</sup> and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5<sup>T</sup> is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5<sup>T</sup> were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5<sup>T</sup> were C<sub>18 : 1</sub> ω7c, C<sub>16 : 0</sub> and C<sub>19 : 0</sub> cyclo ω7c. The major ubiquinone of strain Zidic-5<sup>T</sup> was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5<sup>T</sup> represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5<sup>T</sup> (=LMG 33565<sup>T</sup>=NCAIM B.02686<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/","citationCount":"0","resultStr":"{\"title\":\"Description of <i>Labrys sedimenti</i> sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of <i>Labrys portucalensis</i> as a later heterotypic synonym of <i>Labrys neptuniae</i>.\",\"authors\":\"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt\",\"doi\":\"10.1099/ijsem.0.006778\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-negative strain, designated as Zidic-5<sup>T</sup>, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5<sup>T</sup> belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578<sup>T</sup> (99.13%), followed by <i>Labrys portucalensis</i> F11<sup>T</sup> (99.06%), <i>Labrys methylaminiphilus</i> JLW10<sup>T</sup> (98.58%) and <i>Labrys okinawensis</i> DSM 18385<sup>T</sup> (98.32%). The draft genome sequence of strain Zidic-5<sup>T</sup> is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5<sup>T</sup> and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5<sup>T</sup> is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5<sup>T</sup> were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5<sup>T</sup> were C<sub>18 : 1</sub> ω7c, C<sub>16 : 0</sub> and C<sub>19 : 0</sub> cyclo ω7c. The major ubiquinone of strain Zidic-5<sup>T</sup> was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5<sup>T</sup> represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5<sup>T</sup> (=LMG 33565<sup>T</sup>=NCAIM B.02686<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 5\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006778\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006778","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Description of Labrys sedimenti sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of Labrys portucalensis as a later heterotypic synonym of Labrys neptuniae.
A Gram-stain-negative strain, designated as Zidic-5T, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5T belongs to the genus Labrys, with the highest 16S rRNA gene similarity to Labrys neptuniae LMG 23578T (99.13%), followed by Labrys portucalensis F11T (99.06%), Labrys methylaminiphilus JLW10T (98.58%) and Labrys okinawensis DSM 18385T (98.32%). The draft genome sequence of strain Zidic-5T is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5T and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5T is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of L. neptuniae and L. portucalensis indicated that they belong to the same genomic species, suggesting that L. portucalensis is a later heterotypic synonym of L. neptuniae. Cells of strain Zidic-5T were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5T were C18 : 1 ω7c, C16 : 0 and C19 : 0 cyclo ω7c. The major ubiquinone of strain Zidic-5T was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5T represents a novel species of the genus Labrys; thus, the name of Labrys sedimenti sp. nov. is proposed. The type strain of the species is strain Zidic-5T (=LMG 33565T=NCAIM B.02686T).
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.