从双氯芬酸降解富集培养物中分离的Labrys sedimenti sp. nov.的描述,以及基于基因组的Labrys portucalensis作为Labrys neptuniae后异型同义词的重新分类。

IF 2 3区 生物学 Q4 MICROBIOLOGY
Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt
{"title":"从双氯芬酸降解富集培养物中分离的Labrys sedimenti sp. nov.的描述,以及基于基因组的Labrys portucalensis作为Labrys neptuniae后异型同义词的重新分类。","authors":"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt","doi":"10.1099/ijsem.0.006778","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-stain-negative strain, designated as Zidic-5<sup>T</sup>, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5<sup>T</sup> belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578<sup>T</sup> (99.13%), followed by <i>Labrys portucalensis</i> F11<sup>T</sup> (99.06%), <i>Labrys methylaminiphilus</i> JLW10<sup>T</sup> (98.58%) and <i>Labrys okinawensis</i> DSM 18385<sup>T</sup> (98.32%). The draft genome sequence of strain Zidic-5<sup>T</sup> is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5<sup>T</sup> and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5<sup>T</sup> is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5<sup>T</sup> were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5<sup>T</sup> were C<sub>18 : 1</sub> ω7c, C<sub>16 : 0</sub> and C<sub>19 : 0</sub> cyclo ω7c. The major ubiquinone of strain Zidic-5<sup>T</sup> was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5<sup>T</sup> represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5<sup>T</sup> (=LMG 33565<sup>T</sup>=NCAIM B.02686<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/","citationCount":"0","resultStr":"{\"title\":\"Description of <i>Labrys sedimenti</i> sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of <i>Labrys portucalensis</i> as a later heterotypic synonym of <i>Labrys neptuniae</i>.\",\"authors\":\"Andrea Csépányi, András Táncsics, Márton Pápai, Erzsébet Baka, Erika Tóth, Károly Bóka, Hussein Daood, István Szabó, Balázs Kriszt\",\"doi\":\"10.1099/ijsem.0.006778\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A Gram-stain-negative strain, designated as Zidic-5<sup>T</sup>, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5<sup>T</sup> belongs to the genus <i>Labrys</i>, with the highest 16S rRNA gene similarity to <i>Labrys neptuniae</i> LMG 23578<sup>T</sup> (99.13%), followed by <i>Labrys portucalensis</i> F11<sup>T</sup> (99.06%), <i>Labrys methylaminiphilus</i> JLW10<sup>T</sup> (98.58%) and <i>Labrys okinawensis</i> DSM 18385<sup>T</sup> (98.32%). The draft genome sequence of strain Zidic-5<sup>T</sup> is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5<sup>T</sup> and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5<sup>T</sup> is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of <i>L. neptuniae</i> and <i>L. portucalensis</i> indicated that they belong to the same genomic species, suggesting that <i>L. portucalensis</i> is a later heterotypic synonym of <i>L. neptuniae</i>. Cells of strain Zidic-5<sup>T</sup> were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5<sup>T</sup> were C<sub>18 : 1</sub> ω7c, C<sub>16 : 0</sub> and C<sub>19 : 0</sub> cyclo ω7c. The major ubiquinone of strain Zidic-5<sup>T</sup> was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5<sup>T</sup> represents a novel species of the genus <i>Labrys</i>; thus, the name of <i>Labrys sedimenti</i> sp. nov. is proposed. The type strain of the species is strain Zidic-5<sup>T</sup> (=LMG 33565<sup>T</sup>=NCAIM B.02686<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 5\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062540/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006778\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006778","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从双氯芬酸降解富集培养中分离到一株革兰氏阴性菌株,命名为Zidic-5T,并采用多相法对其进行了鉴定。16S rRNA基因序列分析表明,菌株Zidic-5T属于Labrys属,与Labrys neptuniae LMG 23578T的16S rRNA基因相似性最高(99.13%),其次为portucalensis F11T(99.06%)、Labrys methylaminiphilus JLW10T(98.58%)和Labrys okinawensis DSM 18385T(98.32%)。菌株Zidic-5T基因组草图序列长7.64 Mb,基因组中G+C含量为63.5 mol%。菌株Zidic-5T与其近缘种的同源平均核苷酸同一性和数字DNA-DNA杂交亲缘值均低于物种划分阈值,证实菌株Zidic-5T与其近缘种明显分离。此外,对L. neptuniae和L. portucalensis型菌株的全基因组比较分析表明,它们属于同一基因组种,表明L. portucalensis是L. neptuniae的后异型同义种。菌株Zidic-5T细胞为严格需氧细胞,球粒状,不运动。菌株Zidic-5T的优势脂肪酸为C18: 1 ω7c、C16: 0和C19: 0 cyclo ω7c,占总脂肪酸的10%。菌株Zidic-5T的主要泛醌为Q-10,主要极性脂质为磷脂酰胆碱、二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和氨基磷脂。经多相分析,认为菌株Zidic-5T为Labrys属新种;因此,建议将其命名为Labrys sedimenti sp. nov.。菌株类型为菌株Zidic-5T (=LMG 33565T=NCAIM B.02686T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Description of Labrys sedimenti sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of Labrys portucalensis as a later heterotypic synonym of Labrys neptuniae.

A Gram-stain-negative strain, designated as Zidic-5T, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5T belongs to the genus Labrys, with the highest 16S rRNA gene similarity to Labrys neptuniae LMG 23578T (99.13%), followed by Labrys portucalensis F11T (99.06%), Labrys methylaminiphilus JLW10T (98.58%) and Labrys okinawensis DSM 18385T (98.32%). The draft genome sequence of strain Zidic-5T is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5T and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5T is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of L. neptuniae and L. portucalensis indicated that they belong to the same genomic species, suggesting that L. portucalensis is a later heterotypic synonym of L. neptuniae. Cells of strain Zidic-5T were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5T were C18 : 1 ω7c, C16 : 0 and C19 : 0 cyclo ω7c. The major ubiquinone of strain Zidic-5T was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5T represents a novel species of the genus Labrys; thus, the name of Labrys sedimenti sp. nov. is proposed. The type strain of the species is strain Zidic-5T (=LMG 33565T=NCAIM B.02686T).

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信