Vreelandella Arctic sp. 11和Vreelandella indica sp. 11,分离自北冰洋和西南印度洋沉积物。

IF 2 3区 生物学 Q4 MICROBIOLOGY
Yuwei Weng, Jingyu Xia, Xuying Zhu, Wanpeng Wang
{"title":"Vreelandella Arctic sp. 11和Vreelandella indica sp. 11,分离自北冰洋和西南印度洋沉积物。","authors":"Yuwei Weng, Jingyu Xia, Xuying Zhu, Wanpeng Wang","doi":"10.1099/ijsem.0.006791","DOIUrl":null,"url":null,"abstract":"<p><p>Four Gram-stain-negative bacterial strains which can metabolize alkanes and polycyclic aromatic hydrocarbons were isolated. Strains R06ZXJ2<sup>T</sup> and R09ZXJ6 were isolated from sediments collected in the Arctic Ocean. Strains DY715-9<sup>T</sup> and DY716-13-1 were isolated from surface sediment samples collected by the deep-sea TV grab in the southwestern Indian Ocean. Phylogenomic analyses based on 16S rRNA gene sequences, average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) revealed that the strains formed two distinct lineages within the genus <i>Vreelandella</i>. Their closest relative was <i>Vreelandella maris</i> QX-1<sup>T</sup>, with ANI values of 94.35% (R06ZXJ2<sup>T</sup>) and 95.54% (DY715-9<sup>T</sup>) and dDDH values of 57.00% and 61.80%, respectively. The pairwise ANI (95.47%) and dDDH (60.70%) between the two novel strains further supported their status as separate species. Phenotypic and chemotaxonomic analyses distinguished them from known <i>Vreelandella</i> species through differences in colony morphology, hydrocarbon degradation profiles and polar lipid compositions. The strains grew optimally at 28 °C and pH 7.0 and can degrade alkanes and polycyclic aromatic hydrocarbons. Based on these results, we propose two novel species: <i>Vreelandella arctica</i> sp. nov. (type strain R06ZXJ2<sup>T</sup>=MCCC 1A08467<sup>T</sup>=KCTC 92631<sup>T</sup>) and <i>Vreelandella indica</i> sp. nov. (type strain DY715-9<sup>T</sup>=MCCC 1A12469<sup>T</sup>=KCTC 92628<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Vreelandella arctica</i> sp. nov. and <i>Vreelandella indica</i> sp. nov., isolated from the Arctic Ocean and the Southwest Indian Ocean sediment.\",\"authors\":\"Yuwei Weng, Jingyu Xia, Xuying Zhu, Wanpeng Wang\",\"doi\":\"10.1099/ijsem.0.006791\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Four Gram-stain-negative bacterial strains which can metabolize alkanes and polycyclic aromatic hydrocarbons were isolated. Strains R06ZXJ2<sup>T</sup> and R09ZXJ6 were isolated from sediments collected in the Arctic Ocean. Strains DY715-9<sup>T</sup> and DY716-13-1 were isolated from surface sediment samples collected by the deep-sea TV grab in the southwestern Indian Ocean. Phylogenomic analyses based on 16S rRNA gene sequences, average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) revealed that the strains formed two distinct lineages within the genus <i>Vreelandella</i>. Their closest relative was <i>Vreelandella maris</i> QX-1<sup>T</sup>, with ANI values of 94.35% (R06ZXJ2<sup>T</sup>) and 95.54% (DY715-9<sup>T</sup>) and dDDH values of 57.00% and 61.80%, respectively. The pairwise ANI (95.47%) and dDDH (60.70%) between the two novel strains further supported their status as separate species. Phenotypic and chemotaxonomic analyses distinguished them from known <i>Vreelandella</i> species through differences in colony morphology, hydrocarbon degradation profiles and polar lipid compositions. The strains grew optimally at 28 °C and pH 7.0 and can degrade alkanes and polycyclic aromatic hydrocarbons. Based on these results, we propose two novel species: <i>Vreelandella arctica</i> sp. nov. (type strain R06ZXJ2<sup>T</sup>=MCCC 1A08467<sup>T</sup>=KCTC 92631<sup>T</sup>) and <i>Vreelandella indica</i> sp. nov. (type strain DY715-9<sup>T</sup>=MCCC 1A12469<sup>T</sup>=KCTC 92628<sup>T</sup>).</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"75 5\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006791\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006791","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

分离到了4株革兰氏阴性的能代谢烷烃和多环芳烃的菌株。菌株R06ZXJ2T和R09ZXJ6从北冰洋沉积物中分离得到。菌株DY715-9T和DY716-13-1分别从印度洋西南部深海电视采集的表层沉积物样品中分离得到。基于16S rRNA基因序列、平均nt同源性(ANI)和数字DNA-DNA杂交(dDDH)的系统基因组分析显示,该菌株在Vreelandella属内形成了两个不同的谱系。其最近亲缘系为马氏Vreelandella maris QX-1T, ANI值分别为94.35% (R06ZXJ2T)和95.54% (DY715-9T), dDDH值分别为57.00%和61.80%。两株新菌株的ANI(95.47%)和dDDH(60.70%)的比值进一步支持了它们作为独立种的地位。表型和化学分类分析通过菌落形态、碳氢化合物降解谱和极性脂质组成的差异将它们与已知的Vreelandella物种区分开来。菌株在28℃、pH 7.0条件下生长最佳,能降解烷烃和多环芳烃。基于这些结果,我们提出了两个新种:北极灰蛭(Vreelandella arctica sp. 11 .)(型菌株R06ZXJ2T=MCCC 1A08467T=KCTC 92631T)和印度灰蛭(Vreelandella indica sp. 11 . DY715-9T=MCCC 1A12469T=KCTC 92628T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vreelandella arctica sp. nov. and Vreelandella indica sp. nov., isolated from the Arctic Ocean and the Southwest Indian Ocean sediment.

Four Gram-stain-negative bacterial strains which can metabolize alkanes and polycyclic aromatic hydrocarbons were isolated. Strains R06ZXJ2T and R09ZXJ6 were isolated from sediments collected in the Arctic Ocean. Strains DY715-9T and DY716-13-1 were isolated from surface sediment samples collected by the deep-sea TV grab in the southwestern Indian Ocean. Phylogenomic analyses based on 16S rRNA gene sequences, average nt identity (ANI) and digital DNA-DNA hybridization (dDDH) revealed that the strains formed two distinct lineages within the genus Vreelandella. Their closest relative was Vreelandella maris QX-1T, with ANI values of 94.35% (R06ZXJ2T) and 95.54% (DY715-9T) and dDDH values of 57.00% and 61.80%, respectively. The pairwise ANI (95.47%) and dDDH (60.70%) between the two novel strains further supported their status as separate species. Phenotypic and chemotaxonomic analyses distinguished them from known Vreelandella species through differences in colony morphology, hydrocarbon degradation profiles and polar lipid compositions. The strains grew optimally at 28 °C and pH 7.0 and can degrade alkanes and polycyclic aromatic hydrocarbons. Based on these results, we propose two novel species: Vreelandella arctica sp. nov. (type strain R06ZXJ2T=MCCC 1A08467T=KCTC 92631T) and Vreelandella indica sp. nov. (type strain DY715-9T=MCCC 1A12469T=KCTC 92628T).

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信