Streptococcus hepaticus sp. nov. isolated from the liver of domestic pigs (Sus scrofa domesticus).

IF 2 3区 生物学 Q4 MICROBIOLOGY
Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore
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Abstract

A Gram-positive coccus-shaped bacterium, which could not be identified by classical determinative bacteriology approaches, was isolated from the liver of two pigs in the United Kingdom. Initial testing based on cellular morphology and biochemical characteristics tentatively assigned the isolates to the genus Streptococcus but did not match any previously described species. The analysis of the 16S rRNA sequence determined that the isolates were most closely related to Streptococcus gallinaceus (98.6% identity). Analysis of three further housekeeping genes frequently applied in streptococcal taxonomy, groEL, sodA and rpoB, and a comparison against available type strain sequences confirmed that the isolates were most similar to S. gallinaceus in all cases (84.3%, 86.9% and 90.2% identity, respectively). The comparison of the average nucleotide identity (ANI) and in silico DNA-DNA hybridization values demonstrated that the novel species was distinct from other streptococcal species. Pairwise ANI values revealed that the two studied strains shared a pairwise ANI of 99.25% but were clearly distinct from previously described Streptococcus species (ANI ≤81.1% - best match S. gallinaceus). The taxonomic analysis described confirmed that the two strains represent a novel Streptococcus species for which the name Streptococcus hepaticus sp. nov. is suggested, with strain 20-1249T (=NCTC 15092T=LMG 33498T) as the type strain.

从家猪肝脏分离的11号肝链球菌。
从英国两头猪的肝脏中分离出一种不能通过经典决定性细菌学方法鉴定的革兰氏阳性球菌状细菌。基于细胞形态和生化特征的初步测试初步将分离物确定为链球菌属,但与先前描述的任何物种都不匹配。16S rRNA序列分析表明,分离物与鸡链球菌(Streptococcus gallinaceus)亲缘关系最密切,同源性为98.6%。对groEL、sodA和rpoB这3个常用于链球菌分类的内源基因进行分析,并与现有型菌株序列进行比较,结果表明,所有病例的分离株与鸡链球菌最相似(同源性分别为84.3%、86.9%和90.2%)。平均核苷酸同一性(ANI)和硅DNA-DNA杂交值的比较表明,该新种与其他链球菌不同。两两ANI值显示,两株链球菌的两两ANI值为99.25%,但与先前描述的链球菌明显不同(ANI≤81.1%,最匹配鸡链球菌)。分类分析证实,这两株菌株代表一种新型链球菌,建议将其命名为肝链球菌(Streptococcus hepatcus sp. 11),型菌株为菌株20-1249T (=NCTC 15092T=LMG 33498T)。
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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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