Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore
{"title":"<i>Streptococcus hepaticus</i> sp. nov. isolated from the liver of domestic pigs (<i>Sus scrofa domesticus</i>).","authors":"Miranda J Kirchner, Daniel Loy, Susanna Williamson, Adrian M Whatmore","doi":"10.1099/ijsem.0.006776","DOIUrl":null,"url":null,"abstract":"<p><p>A Gram-positive coccus-shaped bacterium, which could not be identified by classical determinative bacteriology approaches, was isolated from the liver of two pigs in the United Kingdom. Initial testing based on cellular morphology and biochemical characteristics tentatively assigned the isolates to the genus <i>Streptococcus</i> but did not match any previously described species. The analysis of the 16S rRNA sequence determined that the isolates were most closely related to <i>Streptococcus gallinaceus</i> (98.6% identity). Analysis of three further housekeeping genes frequently applied in streptococcal taxonomy, <i>groEL</i>, <i>sodA</i> and <i>rpoB</i>, and a comparison against available type strain sequences confirmed that the isolates were most similar to <i>S. gallinaceus</i> in all cases (84.3%, 86.9% and 90.2% identity, respectively). The comparison of the average nucleotide identity (ANI) and <i>in silico</i> DNA-DNA hybridization values demonstrated that the novel species was distinct from other streptococcal species. Pairwise ANI values revealed that the two studied strains shared a pairwise ANI of 99.25% but were clearly distinct from previously described <i>Streptococcus</i> species (ANI ≤81.1% - best match <i>S. gallinaceus</i>). The taxonomic analysis described confirmed that the two strains represent a novel <i>Streptococcus</i> species for which the name <i>Streptococcus hepaticus</i> sp. nov. is suggested, with strain 20-1249<sup>T</sup> (=NCTC 15092<sup>T</sup>=LMG 33498<sup>T</sup>) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12064851/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006776","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A Gram-positive coccus-shaped bacterium, which could not be identified by classical determinative bacteriology approaches, was isolated from the liver of two pigs in the United Kingdom. Initial testing based on cellular morphology and biochemical characteristics tentatively assigned the isolates to the genus Streptococcus but did not match any previously described species. The analysis of the 16S rRNA sequence determined that the isolates were most closely related to Streptococcus gallinaceus (98.6% identity). Analysis of three further housekeeping genes frequently applied in streptococcal taxonomy, groEL, sodA and rpoB, and a comparison against available type strain sequences confirmed that the isolates were most similar to S. gallinaceus in all cases (84.3%, 86.9% and 90.2% identity, respectively). The comparison of the average nucleotide identity (ANI) and in silico DNA-DNA hybridization values demonstrated that the novel species was distinct from other streptococcal species. Pairwise ANI values revealed that the two studied strains shared a pairwise ANI of 99.25% but were clearly distinct from previously described Streptococcus species (ANI ≤81.1% - best match S. gallinaceus). The taxonomic analysis described confirmed that the two strains represent a novel Streptococcus species for which the name Streptococcus hepaticus sp. nov. is suggested, with strain 20-1249T (=NCTC 15092T=LMG 33498T) as the type strain.
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.