Cyberlindnera hibernica sp. nov. and Barnettozyma discipulorum sp. nov., isolated from forest soil in Ireland.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-09-01 DOI:10.1099/ijsem.0.006898

Adam P Ryan, Cláudia Carvalho, Yiran Zhao, Julianna Decuseara, Matthieu Osborne, Padraic G Heneghan, Kevin P Byrne, Tadhg Ó Cróinín, Kenneth H Wolfe, José Paulo Sampaio, Geraldine Butler

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引用次数: 0

Abstract

Two yeast strains, PYCC 10015 and PYCC 10016, were isolated from soil from an Irish forest. Sequence analysis of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) of the rRNA gene repeat, and the D1/D2 domain of the LSU rRNA gene, showed that they belong to the Cyberlindnera and Barnettozyma genera of the order Phaffomycetales, but they did not exactly match any known species. The genomes of both isolates were sequenced using Oxford Nanopore Technologies and Illumina sequencing, generating chromosome-level genome assemblies. Phylogenomic analysis of 1,385 single-copy orthologues from 37 Phaffomycetales species and 2 outgroups showed that the closest relative of PYCC 10015 is Cyberlindnera galapagoensis and that PYCC 10016 is placed in a subclade containing 7 other species from the Barnettozyma genus. The average nucleotide identity between these strains and their closest relatives is <75%, supporting their designation as novel species. Here, we propose the names Cyberlindnera hibernica sp. nov. and Barnettozyma discipulorum sp. nov. for PYCC 10015 and PYCC 10016, respectively.

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从爱尔兰森林土壤中分离出的cyberlinnera hibernica sp. 11和Barnettozyma disciporum sp. 11。

从爱尔兰森林土壤中分离到两株酵母菌PYCC 10015和PYCC 10016。rRNA基因重复序列的内部转录间隔（ITS）区（ITS1-5.8S-ITS2）和LSU rRNA基因的D1/D2结构域的序列分析表明，它们属于Phaffomycetales目Cyberlindnera和Barnettozyma属，但与任何已知物种都不完全匹配。利用Oxford Nanopore Technologies和Illumina测序技术对两株菌株的基因组进行测序，得到染色体水平的基因组组装。对37个Phaffomycetales种和2个外群的1385个单基因同源物的系统基因组分析表明，PYCC 10015与Cyberlindnera galapagoensis的亲缘关系最近，PYCC 10016被放在一个包含7个Barnettozyma属物种的亚枝中。PYCC 10015和PYCC 10016的平均核苷酸同源性分别为Cyberlindnera hibernica sp. 11和Barnettozyma disciporum sp. 11。

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来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.