Butyricimonas muris sp. nov., isolated from mouse faeces.

Abstract

An extracellular polysaccharide-producing strain labelled GBG-M4^T was isolated from the faeces of a C57BL/6 J mouse model with ulcerative colitis prognosis. Using a polyphasic approach, the taxonomy of this novel species was characterized. The strain was Gram-negative, non-motile, non-spore-forming, non-pigmented and rod-shaped. Strain GBG-M4 ^T grew anaerobically at 30-45℃ (optimally at 37-40℃) and at pH 6.5-8.0 (optimally at pH 7.0-8.0). Growth of the strain was not inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that strain GBG-M4^T represents a member of the genus Butyricimonas. The similarity of the 16S rRNA gene sequence of strain GBG-M4^T to the type species of the genus, Butyricimonas faecihominis JCM 18676^T, was 94.56%. This strain could produce acetic acid, propionic acid, butyric acid, iso-butyric acid, valeric acid and caproic acid as final products from glucose. The major polar lipids were five lipids, three phospholipids, four ammonium phosphatides and one glyceride. The major fatty acids of strain GBG-M4^T were iso-C _{17: 0} 3-OH and iso-C _{15: 0}. Genome analysis revealed that strain GBG-M4^T has a genome size of 5.56 Mb and a DNA G+C content of 45.57 mol%. A total of 4,576 genes were predicted by genome sequence analysis. On the basis of phenotypic, chemotaxonomic and phylogenetic characterization, strain GBG-M4^T represents a novel species within the genus Butyricimonas, for which the name Butyricimonas muris sp. nov. is proposed. The type strain is GBG-M4^T (=CGMCC 1.18113^T=KCTC 25905^T).