Fems Microbiology Letters最新文献_第10页

Theoretical hypothesis in a direct electron transfer between non-interacting Fe-S proteins within an artificial fusion. 人工聚变内非相互作用的 Fe-S 蛋白之间直接电子转移的理论假设。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnad137

Jae Kyu Lim

引用次数: 0

Light irradiation changes the regulation pattern of BtCrgA on carotenogenesis in Blakeslea trispora. 光照改变了三孢钝叶草（Blakeslea trispora）中 BtCrgA 对胡萝卜素生成的调控模式。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae002

Jiamin Yang, Mingxi Zeng, Hui Wu, Zhenlin Han, Zhiyan Rock Du, Xiaobin Yu, Wei Luo

{"title":"Light irradiation changes the regulation pattern of BtCrgA on carotenogenesis in Blakeslea trispora.","authors":"Jiamin Yang, Mingxi Zeng, Hui Wu, Zhenlin Han, Zhiyan Rock Du, Xiaobin Yu, Wei Luo","doi":"10.1093/femsle/fnae002","DOIUrl":"10.1093/femsle/fnae002","url":null,"abstract":"CrgA has been shown to be a negative regulator of carotenogenesis in some filamentous fungi, while light irradiation is an inducible environmental factor for carotenoid biosynthesis. To clarify the relationship between CrgA and light-inducible carotenogenesis in Blakeslea trispora, the cis-acting elements of the btcrgA promoter region were investigated, followed by the analyses of correlation between the expression of btcrgA and carotenoid structural genes under different irradiation conditions. A variety of cis-acting elements associated with light response was observed in the promoter region of btcrgA, and transcription of btcrgA and carotenoid structural genes under different irradiation conditions was induced by white light with a clear correlation. Then, RNA interference and overexpression of btcrgA were performed to investigate their effects on carotenogenesis at different levels under irradiation and darkness. The analyses of transcription and enzyme activities of carotenoid structural gene, and accumulation of carotenoids among btcrgA-interfered, btcrgA-overexpressed, and wild-type strains under irradiation and darkness indicate that btcrgA negatively regulates the synthesis of carotenoid in darkness, while promotes the carotenogenesis under irradiation regardless of reduced or overexpression of btcrgA .","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139416718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of: Disruption of the gene (spooA) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A. 撤回：干扰编码孢子转录因子的基因（spooA）会阻止 A 型肠毒性产气荚膜梭菌内孢子的形成和肠毒素的产生。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae022

引用次数: 0

Sensitivity of Salmonella Typhimurium to nisin in vitro and in orange juice under refrigeration. 体外和冷藏橙汁中的鼠伤寒沙门氏菌对尼生素的敏感性。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae031

Carla Gabriela Fernandes da Silva, Flávio Santos Lopes, Álissy Fernanda Cardoso Vieira Valois, Cláudia Vieira Prudêncio

引用次数: 0

Using "Adopt a Bacterium" as an e-learning tool for simultaneously teaching microbiology to different health-related university courses. 利用 "领养细菌 "作为电子学习工具，同时教授大学不同健康相关课程的微生物学。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae033

Hadassa Cristhina de Azevedo Soares Dos Santos, Bárbara Rodrigues Cintra Armellini, Giovanna Lello Naves, Vanessa Bueris, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira

{"title":"Using \"Adopt a Bacterium\" as an e-learning tool for simultaneously teaching microbiology to different health-related university courses.","authors":"Hadassa Cristhina de Azevedo Soares Dos Santos, Bárbara Rodrigues Cintra Armellini, Giovanna Lello Naves, Vanessa Bueris, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira","doi":"10.1093/femsle/fnae033","DOIUrl":"10.1093/femsle/fnae033","url":null,"abstract":"The COVID-19 pandemic has posed challenges for education, particularly in undergraduate teaching. In this study, we report on the experience of how a private university successfully addressed this challenge through an active methodology applied to a microbiology discipline offered remotely to students from various health-related courses (veterinary, physiotherapy, nursing, biomedicine, and nutrition). Remote teaching was combined with the \"Adopt a Bacterium\" methodology, implemented for the first time on Google Sites. The distance learning activity notably improved student participation in microbiology discussions, both through word cloud analysis and the richness of discourse measured by the Shannon index. Furthermore, feedback from students about the e-learning approach was highly positive, indicating its effectiveness in motivating and involving students in the learning process. The results also demonstrate that despite being offered simultaneously to students, the methodology allowed for the acquisition of specialized knowledge within each course and sparked student interest in various aspects of microbiology. In conclusion, the remote \"Adopt a Bacterium\" methodology facilitated knowledge sharing among undergraduate students from different health-related courses and represented a valuable resource in distance microbiology education.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141093210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MATH test. A three-phase assay? 数学测试。三阶段测试？

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae045

Jesús A Salas-Tovar, Sarai Escobedo-García, Guadalupe I Olivas, Carlos H Acosta-Muñiz, Federico Harte, David R Sepulveda

{"title":"The MATH test. A three-phase assay?","authors":"Jesús A Salas-Tovar, Sarai Escobedo-García, Guadalupe I Olivas, Carlos H Acosta-Muñiz, Federico Harte, David R Sepulveda","doi":"10.1093/femsle/fnae045","DOIUrl":"10.1093/femsle/fnae045","url":null,"abstract":"This study aimed to investigating the possible interference caused by glass test tubes on the quantification of bacterial adhesion to hydrocarbons by the MATH test. The adhesion of four bacteria to hexadecane and to glass test tubes was evaluated employing different suspending polar phases. The role of the ionic strength of the polar phase regarding adhesion to glassware was investigated. Within the conditions studied, Gram-positive bacteria adhered to both the test tube and the hydrocarbon regardless of the polar phase employed; meanwhile, Escherichia coli ATCC 25922 did not attach to either one. The capacity of the studied microorganisms to adhere to glassware was associated with their electron-donor properties. The ionic strength of the suspending media altered the patterns of adhesion to glass in a strain-specific manner by defining the magnitude of electrostatic repulsion observed between bacteria and the glass surface. This research demonstrated that glass test tubes may interact with suspended bacterial cells during the MATH test under specific conditions, which may lead to overestimating the percentage of adhesion to hydrocarbons and, thus, to erroneous values of cell surface hydrophobicity.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141310373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli. 亚胺培南暴露影响大肠杆菌中法定量感应受体 sdiA 的表达。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae096

Chandrayee Deshamukhya, Ferdous Begom, Bhaskar Jyoti Das, Monjur Ahmed Laskar, Sangeeta Goala, Manabendra Dutta Choudhury, Debadatta Dhar Chanda, Amitabha Bhattacharjee

{"title":"Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli.","authors":"Chandrayee Deshamukhya, Ferdous Begom, Bhaskar Jyoti Das, Monjur Ahmed Laskar, Sangeeta Goala, Manabendra Dutta Choudhury, Debadatta Dhar Chanda, Amitabha Bhattacharjee","doi":"10.1093/femsle/fnae096","DOIUrl":"10.1093/femsle/fnae096","url":null,"abstract":"The increasing trend of carbapenem resistance amongst Escherichia coli poses a major public health crisis and requires active surveillance of resistance mechanisms to control the threat. Quorum-sensing system plays a role in bacterial resistance to antibiotics. Quorum sensing is a cell-cell communication system where bacteria alter their gene expression in response to specific stimuli. Here, in this study we investigated the transcriptional response of quorum-sensing receptor, sdiA in E. coli under sub-inhibitory concentration of carbapenem in the presence of quorum-sensing signal molecules. Two E. coli isolates harbouring blaNDM were subjected to treatment with 10% Sodium Dodecyl Sulphate (SDS) for 20 consecutive days of which blaNDM encoding plasmid was successfully eliminated from one isolate. Both the wild type and the cured mutant were then allowed to grow under eight different inducing conditions and the transcriptional response of sdiA gene was studied by quantitative real-time Polymerase Chain Reaction (PCR) methodt. We found different response levels of sdiA in wild type and cured mutant under exogenous AHL and imipenem and when co-cultured with Pseudomonas aeruginosa under imipenem stress. This study highlighted that sub-inhibitory concentration of imipenem in combination with AHL is acting as a signal to SdiA, a quorum-sensing receptor in E. coli.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DIVULSUPERBAC: an outreach project to raise awareness of antimicrobial resistance. DIVULSUPERBAC：提高对抗菌药耐药性认识的外联项目。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae099

Sergi Maicas, Belén Fouz

引用次数: 0

Discovery of a glyphosate oxidase in nature. 在自然界发现草甘膦氧化酶

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae086

Minghui Ma, Afshan Ardalan, Adrian Van Dyk, Trevor C Charles, Geoff P Horsman

{"title":"Discovery of a glyphosate oxidase in nature.","authors":"Minghui Ma, Afshan Ardalan, Adrian Van Dyk, Trevor C Charles, Geoff P Horsman","doi":"10.1093/femsle/fnae086","DOIUrl":"10.1093/femsle/fnae086","url":null,"abstract":"Glyphosate is the most used herbicide on Earth. After a half-century of use we know only two biodegradative pathways, each of which appears to degrade glyphosate incidentally. One pathway begins with oxidation of glyphosate catalysed by glycine oxidase (GO). To date, no naturally occurring GO enzymes preferentially oxidize glyphosate but nonetheless are sufficiently active to initiate its degradation. However, GO enzymes that preferentially oxidize glyphosate over glycine-i.e. glyphosate oxidases (GOXs)-may have evolved in environments facing prolonged glyphosate exposure. To test this hypothesis, we screened a metagenomic library from glyphosate-exposed agricultural soil and identified a GOX from clone 11AW19 (GO19) that prefers glyphosate over glycine by four orders of magnitude. This is the first GO isolated from a natural source exhibiting a glyphosate preference. Not only have we discovered the first GOX in nature, but we have also demonstrated the utility of functional metagenomics to find a GOX with greater catalytic efficiency and specificity than those engineered using directed evolution.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origin tracking of Brucella strain B. melitensics bv.3 ARQ-070 using biochemical and genomic studies. 利用生化和基因组研究追踪布鲁氏菌菌株 B. melitensis bv.3 ARQ-070 的起源。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae085

Na Ta, Ke-Ming Zuo, Jing Gao, Na Guan, Li-Tao Song, Yong-Jun Wen, Rui-Ping Yu

{"title":"Origin tracking of Brucella strain B. melitensics bv.3 ARQ-070 using biochemical and genomic studies.","authors":"Na Ta, Ke-Ming Zuo, Jing Gao, Na Guan, Li-Tao Song, Yong-Jun Wen, Rui-Ping Yu","doi":"10.1093/femsle/fnae085","DOIUrl":"10.1093/femsle/fnae085","url":null,"abstract":"To conduct an origin tracking and genomic study of the Brucella strain B. melitensis bv.3 ARQ-070, with the aim of addressing the challenges posed by the highly conserved genome of Brucella to conventional typing methods and to gain an understanding of the geographic distribution and interspecies transmission of this pathogen in China. Differentiation and genotyping were established via biochemical profiles and polymerase chain reaction. Illumina MiSeq® was applied to sequence the Brucella isolates. Using multilocus sequence typing and Fisher's exact test was used for the KEGG enrichment analysis of differential genes. Differential gene protein-protein interaction (PPI) network analysis was conducted using the STRING database and visualization was performed using. According to the final identification results of an A/M serum agglutination test. ARQ-070 was identified as the No. 3 white spirulina biological variety, and mM was identified as the No. 1 white Spirulina biological variety. The genetic information of this strain is very close to that of the M5 vaccine strain, suggesting possible vaccine-escape infection. In the comparative genomics analysis with B. melitensis bv.1 16 M, the main differences between the B. melitensis strains were found to be concentrated in the genes related to amino acid metabolism and environmental perception. A base mutation was found in the Brucella gene virB9, which is associated with the key virulence factor of the type IV secretion system, but this mutation did not lead to changes in the protein's tertiary structure, and the strain did not lose its infectivity. The study discovered a base mutation in the virB9 gene of Brucella, which is linked to T4SS but does not affect the protein's structure or the strain's infectivity. This mutation could influence public health approaches to detecting and preventing Brucella transmission. Future research aims to analyse a wider range of Brucella strains for a deeper understanding of their epidemiology.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0