Fems Microbiology Letters最新文献_第10页

Slx5/Slx8 SUMO-targeted ubiquitin ligase deficiency shortens lifespan due to increased mutation accumulation in yeast. Slx5/Slx8 sumo靶向泛素连接酶缺乏症由于酵母突变积累增加而缩短寿命。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae109

Pınar B Thomas, Nur Kaluç, Irmak N Çavlı, Bilge G Tuna

{"title":"Slx5/Slx8 SUMO-targeted ubiquitin ligase deficiency shortens lifespan due to increased mutation accumulation in yeast.","authors":"Pınar B Thomas, Nur Kaluç, Irmak N Çavlı, Bilge G Tuna","doi":"10.1093/femsle/fnae109","DOIUrl":"10.1093/femsle/fnae109","url":null,"abstract":"Chronological lifespan (CLS) in budding yeast Saccharomyces cerevisiae, which is defined as the time nondividing cells in saturation remain viable, has been utilized as a model to study post-mitotic aging in mammalian cells. CLS is closely related to entry into and maintenance of a quiescent state. Many rearrangements that direct the quiescent state enhance the ability of cells to endure several types of stress. Small ubiquitin-like modifier (SUMO)-targeted ubiquitin ligases (STUbLs) play a critical role in mediating an adaptive response to various stresses. In this study, we investigated the effect of a STUbL, Slx5/Slx8, on CLS in budding yeast. We showed that both SLX5 and SLX8 deletions accelerate chronological aging, resulting in a decreased maximum and mean lifespan. slx5Δ cells were capable of entering or maintaining a quiescent state during aging. On the other hand, aging slx5Δ and slx8Δ cells had both increased spontaneous mutation accumulation. Our data together indicate that Slx5/Slx8 STUbL is required for normal rate of aging by preventing increased spontaneous mutation accumulation during aging.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and enzymatic profiling reveals aminopeptidase potential of Lactobacillus acidophilus ItalPN270. 遗传和酶分析揭示了嗜酸乳杆菌ItalPN270的氨基肽酶潜力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf028

Cristian Mauricio Barreto Pinilla, Frank Guzman Escudero, Leila Maria Spadoti, Adriano Brandelli, Adriana Torres Silva E Alves

{"title":"Genetic and enzymatic profiling reveals aminopeptidase potential of Lactobacillus acidophilus ItalPN270.","authors":"Cristian Mauricio Barreto Pinilla, Frank Guzman Escudero, Leila Maria Spadoti, Adriano Brandelli, Adriana Torres Silva E Alves","doi":"10.1093/femsle/fnaf028","DOIUrl":"10.1093/femsle/fnaf028","url":null,"abstract":"Lactobacillus acidophilus strains are considered probiotics and have several industrial applications, including their use as non-starter cultures in fermented milk products. However, their biotechnological potential was partially explored. This work investigated the potential peptidase activity of Lactobacillus acidophilus ItalPN270, by mining their whole genome for genetically encoded peptidases and a comparative in vitro analysis of aminopeptidase activity and lytic behavior. The results showed that the assembled bacterial genome comprised one circular chromosome (1 964 524 bp) with 34.57% GC content, and 1906 protein-coding sequences (CDSs). Analysis of the genome sequence of ItalPN270 revealed the presence of 25 genes that encode peptidases with different specificities. The ItalPN270 presented higher values of aminopeptidase activity in vitro, regarding the six enzymatic substrates evaluated, showing values of total aminopeptidase activity 4-fold higher, as compared with an L. paracasei and L. helveticus strains, and notable high activity of pepA, pepL, and pepX. Moreover, the strain ItalPN270 showed an autolysis profile defined by 63.4% of lysis in the first 5 days with low variations after 40 days at 13°C. Thus, our results indicated that strain L. acidophilus ItalPN270 is a potential source of peptidases for different applications, including as adjunct bacteria for improving cheese ripening.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola. 在橡胶树白粉病真菌Erysiphe quercicola中，dicer样基因是有效侵染植物所必需的。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf032

Lijuan He, Ao Guo, Xiaoli Li, Xinze Xu, Yuxiao Fang, Zhigang Li, Wenbo Liu, Xiao Li, Weiguo Miao, Daipeng Chen

{"title":"Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola.","authors":"Lijuan He, Ao Guo, Xiaoli Li, Xinze Xu, Yuxiao Fang, Zhigang Li, Wenbo Liu, Xiao Li, Weiguo Miao, Daipeng Chen","doi":"10.1093/femsle/fnaf032","DOIUrl":"10.1093/femsle/fnaf032","url":null,"abstract":"The obligate parasitic fungus Erysiphe quercicola is the causal agent of powdery mildew of rubber tree (Hevea brasiliensis), posing a significant threat to the rubber tree growth and latex production. Pathogens secrete sRNAs that are crucial virulence factors capable of suppressing plant immunity. Dicer-like proteins (DCLs), as endoribonucleases, play a pivotal role in sRNA biosynthesis. However, the function of DCL in E. quercicola remains unclear. In this study, we identified and characterized two DCLs from E. quercicola, EqDCL1 and EqDCL2. Protein domain analysis and phylogenetic analysis confirmed that EqDCL1 and EqDCL2 are typical DCL proteins. Gene expression analysis revealed that EqDCL1 and EqDCL2 were induced at 10 hpi during the peak of appressorium formation. HIGS targeting EqDCL1, EqDCL2, or both EqDCL1 and EqDCL2 resulted in reduced pathogenicity of E. quercicola and increased immune responses in plants after infection. Overall, our findings demonstrate the essential role of EqDCLs during the infection process of E. quercicola, providing a foundation for further studies on RNAi mechanisms and pathogenic sRNAs in E. quercicola.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Priestia and Phytobacter sp. prevent membrane damage and electrolyte leakage from Capsicum annuum L. seeds subjected to sub-optimal temperature stress. Priestia和Phytobacter sp.可以防止辣椒种子在次优温度胁迫下膜损伤和电解质泄漏。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf033

Prima Bagui, Poulomi Pal, Nipa Biswas, Bratati Chowdhury, Binayak Chakraborty, Prithwiraj Dey, Kapudeep Karmakar

{"title":"Priestia and Phytobacter sp. prevent membrane damage and electrolyte leakage from Capsicum annuum L. seeds subjected to sub-optimal temperature stress.","authors":"Prima Bagui, Poulomi Pal, Nipa Biswas, Bratati Chowdhury, Binayak Chakraborty, Prithwiraj Dey, Kapudeep Karmakar","doi":"10.1093/femsle/fnaf033","DOIUrl":"10.1093/femsle/fnaf033","url":null,"abstract":"Unlike the Himalayas, the sub-Himalayan zones did not experience snowfall and thus suitable for growing solanaceous vegetables. However, several cold waves have been reported to affect the district of Coochbehar (West Bengal, India), which belongs to the Cwa zone (as per Koppen's classification). Variable duration of sub-optimal soil temperature can have a detrimental effect on the growth of seedlings. Our previous study demonstrates that a constant temperature of 20°C (6 degrees below the optimal soil temperature) causes a 71% loss of vigor in seeds of solanaceous plants. Since the soil temperature is not constant diurnally, it was hypothesized that the duration of cold stress can have variable effects on vigor of Capsicum annuum L. It was observed that increasing the duration of cold stress (18 °C) up to 2 hours/day can improve the vigor but after 6 hours/day, a significant drop in vigor was observed. This was because of the cold-associated membrane damage leading to the leakage of electrolytes. To date, this stress existing in these regions has gone unnoticed. In this regard, biopriming the seeds with exopolysaccharide (EPS)-producing microbes can be useful as the EPS can form a protective layer on the seeds. Two lesser-known bacteria namely, Phytobacter and Priestia sp. were evaluated for their vigor-recovering ability. Treatment of seed with these microbes reduced the electrolyte leakage which improved the vigor under sub-optimal stress. This was also validated by fluorescent microscopy. Both these strains displayed an enhanced EPS-producing ability at 18°C which correlated with the reduced electrolyte leakage and enhanced stability of cell membrane. Such bacteria can help in promoting seed vigor under sub-optimal temperature stress.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking plastic degradation potential and resistance gene abundance in bacterioplankton community of the Sundarbans estuarine ecosystem. 孙德尔本斯河口生态系统浮游细菌群落塑料降解潜力与抗性基因丰度的关联

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf067

Nirupama Saini, Anwesha Ghosh, Punyasloke Bhadury

{"title":"Linking plastic degradation potential and resistance gene abundance in bacterioplankton community of the Sundarbans estuarine ecosystem.","authors":"Nirupama Saini, Anwesha Ghosh, Punyasloke Bhadury","doi":"10.1093/femsle/fnaf067","DOIUrl":"10.1093/femsle/fnaf067","url":null,"abstract":"Harnessing microbial capabilities offers a promising and sustainable approach to address the global challenge of plastic waste. However, the potential of mangrove microbiomes to degrade diverse plastic polymers remains largely unexplored. In this metagenomic-based study, surface water microbiomes were analysed from the Indian Sundarbans, part of the world's largest contiguous mangrove ecosystem, revealing 748.21 hits per billion nucleotides associated with plastic-degrading enzymes (PDEs) targeting 17 different polymer types. Of these, 72.9% corresponded to synthetic polymers and 27.1% to natural polymers. The highest number of hits (223) was associated with polyethylene glycol-degrading enzymes, representing 26.7% of the total PDEs hits. Taxonomic analysis revealed Deltaproteobacteria and Gammaproteobacteria as key degraders of diverse synthetic plastic polymers, with Deltaproteobacteria emerging as a previously unreported group. This suggests that surface sediments may serve as reservoirs for novel plastic-degrading microbes. Co-occurrence network analysis indicated possible emerging co-selection or complex associations between PDEs, antibiotic resistance genes (ARGs), and metal resistance genes (MRGs). Notably, zinc resistance genes and aminoglycoside-related ARGs showed more associations with PDEs. While the presence of PDEs offers a promising avenue for bioremediation, their application may be complicated by the concurrent rise of ARGs and MRGs within PDE-harbouring microbes. Thus, it highlights the need for careful assessment when employing microbes for plastic bioremediation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobilizable multidrug resistance plasmid identified in Staphylococcus xylosus isolated from fermented soybean food. 发酵大豆食品中木糖葡萄球菌可动员多药耐药质粒的鉴定。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf068

Sumin Seo, Do-Won Jeong, Sooyoung Sul, Jong-Hoon Lee

{"title":"Mobilizable multidrug resistance plasmid identified in Staphylococcus xylosus isolated from fermented soybean food.","authors":"Sumin Seo, Do-Won Jeong, Sooyoung Sul, Jong-Hoon Lee","doi":"10.1093/femsle/fnaf068","DOIUrl":"10.1093/femsle/fnaf068","url":null,"abstract":"We identified two Staphylococcus xylosus strains that were isolated from the same Korean fermented soybean food with different antibiotic resistance (AR) profiles. Strain 14BME10 showed multidrug resistance to erythromycin and tetracycline, whereas strain 14BME18 was resistant to tetracycline only. We sought to elucidate the genetic background of the AR in these strains. A comparative genomic analysis with three other antibiotic-sensitive S. xylosus strains revealed AR determinants located on two mobilizable plasmids in the isolates. The 73.7-kb plasmid p14BME10-1, encoding the ABC-F-type ribosomal protection protein gene msr(A) and the Mph(C) family macrolide 2'-phosphotransferase gene mph(C), might have contributed to erythromycin resistance. The 4.4-kb plasmid harboring the tetracycline efflux major facilitator superfamily (MFS) transporter gene tet(K) conferred tetracycline resistance and showed 100% DNA sequence identity with the prototype tetracycline resistance plasmid pT181, widely identified in staphylococci. We discovered that the MOBP relaxase gene cluster in p14BME10-1 consists of four genes and an oriT, which might be involved in plasmid mobilization and was also found in many staphylococcal plasmids. The plasmid p14BME10-1 harbors multiple genes for replication, mobilization, transposition, recombination, and resistance to erythromycin, streptomycin, and bacitracin, indicating several occurrences of recombination and integration events but lacks the genes necessary for conjugal transfer.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice husk- and lemongrass-derived eco-enzymes as potential food contact surface disinfectants against biofilm-forming foodborne pathogens. 稻壳和柠檬草衍生的生态酶作为潜在的食品接触面消毒剂对抗生物膜形成的食源性病原体。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae116

Vickneish Vimalanathan, Hanan Hasan, Vickineshwari Kunasegaran, Kausalyaa Sarawanan, Monisha Ilangovan, Pratheep Sandrasaigaran

{"title":"Rice husk- and lemongrass-derived eco-enzymes as potential food contact surface disinfectants against biofilm-forming foodborne pathogens.","authors":"Vickneish Vimalanathan, Hanan Hasan, Vickineshwari Kunasegaran, Kausalyaa Sarawanan, Monisha Ilangovan, Pratheep Sandrasaigaran","doi":"10.1093/femsle/fnae116","DOIUrl":"10.1093/femsle/fnae116","url":null,"abstract":"This study aims to evaluate the rice husk (EE-R)- and lemongrass (EE-L)-derived eco-enzymes (EE) as alternatives to chemical-based disinfectants. The EE-R's and EE-L's antimicrobial activity were tested against Pseudomonas aeruginosa, Salmonella Typhimurium, and Staphylococcus aureus using a broth microdilution method. The antibiofilm activities of EE were determined using crystal violet staining. Lastly, the minimal contact time of EE for effectively reducing biofilm-forming pathogens (<25 CFU/ml) was assessed on various food contact surfaces (wood, glass, plastic, stainless steel, and marble). The results show that EE-R at 25%-50% concentration significantly inhibited P. aeruginosa and S. aureus while reducing the initial biofilm formation by 61% and 58%, respectively. In contrast, EE-L inhibited S. Typhimurium at a concentration of 12.5%-50% and P. aeruginosa at 25%-50%, with a strong preformed biofilm inhibition noticed for S. Typhimurium (70%). For the minimal contact time, EE-R superiorly inhibited P. aeruginosa (60 s) and S. aureus (120 s) on all contact surfaces, while EE-L needed 120 s to reduce P. aeruginosa and S. Typhimurium. These outcomes were comparable to sodium hypochlorite (NaOCl, 2.5%). The study's outcomes implicate the potential application of EE-R and EE-L as surface disinfectants against biofilm-forming bacteria, thus promoting safer food processing practices while minimizing environmental impacts.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MSMEG_3978 (BlaE) from Mycobacterium smegmatis is an extended-spectrum beta-lactamase with a critical tyrosine residue governing its deacylation. 来自耻垢分枝杆菌的MSMEG_3978 （BlaE）是一种广谱β -内酰胺酶，具有关键的酪氨酸残基控制其去酰化。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf098

Aditya Prasad Panda, Anik Ghosh, Sarmista Biswal, Anindya S Ghosh

{"title":"MSMEG_3978 (BlaE) from Mycobacterium smegmatis is an extended-spectrum beta-lactamase with a critical tyrosine residue governing its deacylation.","authors":"Aditya Prasad Panda, Anik Ghosh, Sarmista Biswal, Anindya S Ghosh","doi":"10.1093/femsle/fnaf098","DOIUrl":"10.1093/femsle/fnaf098","url":null,"abstract":"The growing success of beta-lactam and beta-lactamase inhibitor combinations against mycobacterial infections emphasizes the need to deepen our understanding of beta-lactamase-mediated resistance in mycobacteria. In this study, we characterize MSMEG_3978 (BlaE), a class C beta-lactamase from Mycobacterium smegmatis, providing the first biochemical and mechanistic evidence of its extended-spectrum activity. Heterologous expression of blaE in Escherichia coli ΔampC strain led to a two-to-four-fold increase in resistance to penicillins and a four-to-eight-fold increase in resistance against cephalosporins, including third-generation oxyimino-cephalosporins and imipenem. Purified BlaE enzyme efficiently hydrolysed a broad range of beta-lactam antibiotics and was resistant to clavulanic acid inhibition, consistent with group 1e class C beta-lactamases. Site-directed mutagenesis of the conserved Tyrosine 170 (Y170) to Phenylalanine (F) impaired deacylation but not acylation, implicating a specific catalytic role for the Y170 residue. Thermal shift assays and molecular dynamics simulations revealed reduced stability of the Y170F mutant protein relative to wild-type BlaE, although ligand binding remained largely unaffected. Collectively, our findings established MSMEG_3978 as an extended-spectrum class C beta-lactamase and identified the residue Y170 as a potential general base contributing to the deacylation process and strengthened our understanding of adaptive beta-lactam resistance in mycobacteria.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces. 用于取样方法的消毒剂中和缓冲液对表面粘附单核增生李斯特菌细胞活力的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf048

Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet

{"title":"Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces.","authors":"Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet","doi":"10.1093/femsle/fnaf048","DOIUrl":"10.1093/femsle/fnaf048","url":null,"abstract":"This study assessed the impact of six disinfectant-neutralizing buffers on Listeria monocytogenes adhering to stainless steel surfaces treated with quaternary ammonium or hydrogen peroxide-based disinfectants. The goal was to evaluate potential stressors induced by these buffers during sampling, minimizing false negatives in food industry surface monitoring. Neutralizing buffers are essential in preserving bacterial viability during sample transport by counteracting residual disinfectants. Listeria monocytogenes populations were quantified immediately after sampling and after 24-h incubation at 8°C, simulating transport conditions. While neutralizing buffers had minimal impact on untreated adherent cells, they significantly reduced mortality in disinfectant-treated cells. However, most buffers failed to preserve viable culturable (VC) populations after disinfection, instead promoting viable but non-culturable (VBNC) states. Notably, prolonged incubation in the Sponge neutralizer facilitated VC population recovery, likely through VBNC resuscitation or VC growth. In contrast, other buffers inhibited recovery, suggesting detrimental effects on stressed cells due to their chemical composition. These findings underscore the importance of selecting appropriate neutralizing buffers in L. monocytogenes detection, influencing food safety surveillance and risk assessment protocols.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12202139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal precipitation in bio-slurry: effects of urea and calcium chloride using non-growing Sporosarcina pasteurii. 生物浆中的结晶沉淀：尿素和氯化钙对未生长的巴氏孢杆菌的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf083

Narjes Gandali Mostafa, Gholam Reza Ghezelbash, Mohammad Shafiei

{"title":"Crystal precipitation in bio-slurry: effects of urea and calcium chloride using non-growing Sporosarcina pasteurii.","authors":"Narjes Gandali Mostafa, Gholam Reza Ghezelbash, Mohammad Shafiei","doi":"10.1093/femsle/fnaf083","DOIUrl":"10.1093/femsle/fnaf083","url":null,"abstract":"Calcite precipitation by non-growing cells occurs more rapidly in the laboratory than with proliferative cells. Sporosarcina pasteurii was grown in urea-containing nutrient broth, and then harvested, washed, and resuspended in a solution. This bacterial suspension was mixed with an equal volume of a reactant solution containing calcium chloride and urea at different concentrations. The precipitate formed was collected, dried, and analyzed using X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy, field emission scanning electron microscopy, and particle size analysis (PSA). This study investigated the effects of urea and calcium chloride concentrations on the type and size of the crystals formed. The most effective concentrations of urea and calcium chloride were both 0.3 M. Semi-quantitative XRD analysis revealed that 97% of the precipitate was calcite, with only 3% being vaterite at these concentrations. PSA results indicated that, in a 0.3 M solution of urea and calcium chloride, most particles ranged in size from 0 to 60 nm. The results show that higher reactant concentrations lead to more precipitate in the slurry, but with a lower percentage of calcite; additionally, the size of the calcite crystals also increases. This study not only reaffirms the ability of non-growing cells to precipitate calcite but also investigates the precise relationship between reactant concentrations, crystal type, and particle size. These insights provide a deeper understanding of bio-slurry and its potential applications in fields like concrete and soil enhancement.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144845065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0