{"title":"Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola.","authors":"Lijuan He, Ao Guo, Xiaoli Li, Xinze Xu, Yuxiao Fang, Zhigang Li, Wenbo Liu, Xiao Li, Weiguo Miao, Daipeng Chen","doi":"10.1093/femsle/fnaf032","DOIUrl":null,"url":null,"abstract":"<p><p>The obligate parasitic fungus Erysiphe quercicola is the causal agent of powdery mildew of rubber tree (Hevea brasiliensis), posing a significant threat to the rubber tree growth and latex production. Pathogens secrete sRNAs that are crucial virulence factors capable of suppressing plant immunity. Dicer-like proteins (DCLs), as endoribonucleases, play a pivotal role in sRNA biosynthesis. However, the function of DCL in E. quercicola remains unclear. In this study, we identified and characterized two DCLs from E. quercicola, EqDCL1 and EqDCL2. Protein domain analysis and phylogenetic analysis confirmed that EqDCL1 and EqDCL2 are typical DCL proteins. Gene expression analysis revealed that EqDCL1 and EqDCL2 were induced at 10 hpi during the peak of appressorium formation. HIGS targeting EqDCL1, EqDCL2, or both EqDCL1 and EqDCL2 resulted in reduced pathogenicity of E. quercicola and increased immune responses in plants after infection. Overall, our findings demonstrate the essential role of EqDCLs during the infection process of E. quercicola, providing a foundation for further studies on RNAi mechanisms and pathogenic sRNAs in E. quercicola.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fems Microbiology Letters","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/femsle/fnaf032","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The obligate parasitic fungus Erysiphe quercicola is the causal agent of powdery mildew of rubber tree (Hevea brasiliensis), posing a significant threat to the rubber tree growth and latex production. Pathogens secrete sRNAs that are crucial virulence factors capable of suppressing plant immunity. Dicer-like proteins (DCLs), as endoribonucleases, play a pivotal role in sRNA biosynthesis. However, the function of DCL in E. quercicola remains unclear. In this study, we identified and characterized two DCLs from E. quercicola, EqDCL1 and EqDCL2. Protein domain analysis and phylogenetic analysis confirmed that EqDCL1 and EqDCL2 are typical DCL proteins. Gene expression analysis revealed that EqDCL1 and EqDCL2 were induced at 10 hpi during the peak of appressorium formation. HIGS targeting EqDCL1, EqDCL2, or both EqDCL1 and EqDCL2 resulted in reduced pathogenicity of E. quercicola and increased immune responses in plants after infection. Overall, our findings demonstrate the essential role of EqDCLs during the infection process of E. quercicola, providing a foundation for further studies on RNAi mechanisms and pathogenic sRNAs in E. quercicola.
期刊介绍：
FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered.
2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020)
Ranking: 98/135 (Microbiology)
The journal is divided into eight Sections:
Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies)
Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens)
Biotechnology and Synthetic Biology
Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses)
Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies)
Virology (viruses infecting any organism, including Bacteria and Archaea)
Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature)
Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology)
If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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