Fems Microbiology Letters最新文献

Effects of compost amendments and experimental drought on grassland soil microbial communities. 堆肥改良剂和试验干旱对草地土壤微生物群落的影响

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-06 DOI: 10.1093/femsle/fnaf108

Daniela Guasconi, Gustaf Hugelius, Karina E Clemmensen, Sara A O Cousins, Jaanis Juhanson, Stefano Manzoni, Nina Roth, Petra Fransson

{"title":"Effects of compost amendments and experimental drought on grassland soil microbial communities.","authors":"Daniela Guasconi, Gustaf Hugelius, Karina E Clemmensen, Sara A O Cousins, Jaanis Juhanson, Stefano Manzoni, Nina Roth, Petra Fransson","doi":"10.1093/femsle/fnaf108","DOIUrl":"https://doi.org/10.1093/femsle/fnaf108","url":null,"abstract":"Prolonged drought is a major stressor for grassland ecosystems. In addition to decreasing plant productivity, it can affect soil microbial activities and thus destabilize nutrient cycling and carbon (C) sequestration. Soil organic amendments (OAs), such as compost, can be used to enhance soil fertility and mitigate drought effects. In this study, we evaluated the responses of fungal and bacterial communities to a 3-year-long experimental drought and compost treatment across four soil depths in two Swedish grasslands and at an upper and a lower topographic position. Results showed that while drought reduced soil moisture and compost amendment increased C content in the topsoil, the effects on microbial abundance and community composition within this time frame were weak, and detectable only in the topsoil. Fungal abundance increased with compost addition, which also affected community composition, while fungal communities were resistant to drought. Bacterial communities were not significantly affected by any of the treatments. This suggests that microbial ecosystem functions were resistant to the experimentally reduced precipitation. Overall, variation between sampling sites were more important for microbial community composition than treatments, highlighting the need for a better understanding of small-spatial-scale environmental controls on soil microbial and plant communities and their ecosystem functions.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-occurrence of Antibiotic, Heavy Metal, and Disinfectant Resistance in Foodborne Staphylococcus aureus Isolates from Poultry, Meat, and Processed Foods. 从家禽、肉类和加工食品中分离出的食源性金黄色葡萄球菌中抗生素、重金属和消毒剂耐药性的共存

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-06 DOI: 10.1093/femsle/fnaf109

Bahar Onaran Acar, Hakan Şenarısoy, Hidayet Bozdoğan

{"title":"Co-occurrence of Antibiotic, Heavy Metal, and Disinfectant Resistance in Foodborne Staphylococcus aureus Isolates from Poultry, Meat, and Processed Foods.","authors":"Bahar Onaran Acar, Hakan Şenarısoy, Hidayet Bozdoğan","doi":"10.1093/femsle/fnaf109","DOIUrl":"https://doi.org/10.1093/femsle/fnaf109","url":null,"abstract":"Staphylococcus aureus is a major foodborne pathogen capable of developing resistance to multiple antimicrobial agents, including antibiotics, disinfectants, and heavy metals. This study investigated 63 S. aureus isolates recovered from poultry products, meat cuts, and processed or ready-to-eat foods for their resistance to selected disinfectants [(benzalkonium chloride (BC), cetylpyridinium chloride (CPC), triclosan (TCS)] and heavy metals [zinc (Zn), nickel (Ni), mercury (Hg), copper (Cu), cadmium (Cd), lead (Pb), cobalt (Co)]. The isolates from processed and ready-to-eat foods exhibited significantly higher resistance scores to antibiotics, disinfectants, and heavy metals compared to those from meat and poultry (p < 0.001). Notably, 45% of isolates from processed foods were resistant to 8 out of 10 tested agents, and enterotoxigenic isolates displayed significantly elevated resistance profiles across all tested agent classes (antibiotics, heavy metals, disinfectants) (U = 99.0, p < 0.001). These findings highlight the potential role of food processing environments in selecting for multidrug-resistant and virulent S. aureus strains. The co-occurrence of resistance to antibiotics, disinfectants, and heavy metals suggests the involvement of co-selection mechanisms, emphasizing the need for integrated surveillance and targeted interventions to reduce the spread of resistant pathogens in the food chain.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Not all "Candida" are "Candida": Nakaseomyces glabratus (H.W. Anderson) Sugita& M. Takask. 2022 onychomycosis and the need for rapid diagnostic. 并非所有的“念珠菌”都是“念珠菌”：光秃中aseomyces (H.W. Anderson) Sugita& M. Takask. 2022甲真菌病和快速诊断的必要性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-03 DOI: 10.1093/femsle/fnaf107

Mirko Benvenuti, Giulia Gasparini, Antonino Torino, Emanuele Claudio Cozzani

引用次数: 0

Advances in our understanding of the lung-gut axis in tuberculosis. 结核病中肺肠轴的认识进展。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-03 DOI: 10.1093/femsle/fnaf106

Skyler Colwell, Jorge Cervantes

{"title":"Advances in our understanding of the lung-gut axis in tuberculosis.","authors":"Skyler Colwell, Jorge Cervantes","doi":"10.1093/femsle/fnaf106","DOIUrl":"https://doi.org/10.1093/femsle/fnaf106","url":null,"abstract":"Human and animal studies have supported the existence of the gut-lung axis, where gastrointestinal commensals and their products can modulate lung immune function. The role of a balanced healthy gut microbiota, and its restoration may impact the microbiota in the lung. Yet the robustness of this evidence varies considerably, with many studies having considerable limitations. Intestinal microbiota diversity is decreased in pulmonary TB patients, and changes in the intestinal microbiota after M. tuberculosis. infection have been reported, underscoring the bidirectionality of the lung-gut axis. These changes may be associated with the progression of TB, influencing the microbiota and immunity homeostasis in those receiving anti-TB treatment. The crosstalk between the gut and the lung is increasingly recognized as a key modulator in the development, progression, and treatment outcomes of tuberculosis (TB). A protective role of the intestinal microbiota against lung infections through its role in macrophage activation, makes it a promising approach as anti-TB adjunct therapy. This mini review synthesizes current understanding of gut-lung interactions in TB pathogenesis, appraising strengths and limitations of the literature, clarifying areas of consensus versus speculation, highlighting where findings remain preliminary, summarizing the impact of anti-TB treatment on microbial communities, and discussing future directions for microbiota-informed interventions to improve patient outcomes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A semi-selective differential medium for identifying strains of Pantoea from environmental samples. 一种从环境样品中鉴定泛菌菌株的半选择性差示培养基。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-01 DOI: 10.1093/femsle/fnaf103

Adria Bateman, Abigail Apperley, John Stavrinides

{"title":"A semi-selective differential medium for identifying strains of Pantoea from environmental samples.","authors":"Adria Bateman, Abigail Apperley, John Stavrinides","doi":"10.1093/femsle/fnaf103","DOIUrl":"https://doi.org/10.1093/femsle/fnaf103","url":null,"abstract":"The genus Pantoea of the Erwinaceae is a genetically and metabolically diverse group whose representatives span a broad range of clinical and non-clinical environments. We sought to develop a culturing medium to facilitate the identification of Pantoea from environmental samples, and to distinguish its members from other closely related Gram-negative genera. We developed a semi-selective differential medium, Pantoea Differential Medium, which contains crystal violet, sodium citrate, tryptone, as well as protease peptone. The efficacy of the medium was evaluated by assessing the growth and phenotype of 42 bacterial strains, including 18 strains of Pantoea along with other representatives of the Enterobacterales. All Pantoea strains, as well as Kosakonia sp. and Duffyella gerundensis formed orange-yellow pigmented colonies on the medium, while all other representative members of the Enterobacterales evaluated formed purple to pink colonies. The medium was also used to distinguish Pantoea from other bacteria in mixed cultures from environmental samples, with 44 of the 48 orange-yellow colonies being identified as members of Pantoea. Pantoea Differential Medium provides a means for rapidly identifying members of Pantoea from environmental samples, and differentiating them from many of the closely related members of the Erwiniaceae.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intratumoral fungus N. crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix. 瘤内真菌通过调节细胞外基质与卵巢癌预后恶化相关。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-10-01 DOI: 10.1093/femsle/fnaf105

Wenqi Fan, Dengwei Zhang, Yuqing Li, Haiyan Wang, Yong-Xin Li, Bin Li, Shien Zou, Chenchen Feng, Ting Guo

{"title":"Intratumoral fungus N. crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix.","authors":"Wenqi Fan, Dengwei Zhang, Yuqing Li, Haiyan Wang, Yong-Xin Li, Bin Li, Shien Zou, Chenchen Feng, Ting Guo","doi":"10.1093/femsle/fnaf105","DOIUrl":"https://doi.org/10.1093/femsle/fnaf105","url":null,"abstract":"Objective: Landmark studies on intratumoral fungi (ITF) have raised concerns due to irreproducible results and data-analysis errors. We aimed to determine whether ITF exist in ovarian cancer (OvCa) and, if so, whether they play a role in disease biology.Methods: Formalin-fixed, paraffin-embedded (FFPE) OvCa samples and multiple controls underwent operational decontamination, qPCR, ITS sequencing, and post hoc data decontamination. We also leveraged updated fungal reads from TCGA generated by the TCMbio group, which addressed human-read contamination and artificial inflation, to validate findings and assess prognostic associations. A murine syngeneic OVHM model with intratumoral N. crassa injection was established. Transcriptomic and metabolomic analyses were performed to explore mechanisms.Results: Tumor-containing blocks harbored significantly higher fungal loads than environmental controls but had loads comparable to paraffin controls. Applying a two-pass decontamination filter reduced raw sequence features from 9,289 ASVs to 659 ASVs. We focused on high-abundance features present in human tissues but absent from xenografts and paraffin controls and identified one candidate, N. crassa, associated with unfavorable prognosis in OvCa. Integrating human and murine data, we found Neurospora correlated with eosinophils, whereas N. crassa itself was not immune-related. N. crassa promoted OvCa progression with downregulation of ILK and decreased ECM-receptor interaction.Conclusions: Most ITF signals are likely contaminants. We identified N. crassa as associated with unfavorable prognosis in OvCa, potentially via modulation of the extracellular matrix.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145198865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive compounds from deep-sea extremophiles: emerging potential in cosmeceuticals and nutraceuticals. 深海极端微生物的生物活性化合物：药妆和营养保健品的新兴潜力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-09-25 DOI: 10.1093/femsle/fnaf102

Bin Ma, Yihe Bu, Jing Huang, Yi Liu, Zihan Guo, Hongfei Yu, Tingting Liang, Dingkang Wang

{"title":"Bioactive compounds from deep-sea extremophiles: emerging potential in cosmeceuticals and nutraceuticals.","authors":"Bin Ma, Yihe Bu, Jing Huang, Yi Liu, Zihan Guo, Hongfei Yu, Tingting Liang, Dingkang Wang","doi":"10.1093/femsle/fnaf102","DOIUrl":"https://doi.org/10.1093/femsle/fnaf102","url":null,"abstract":"The deep sea contains a varied range of extremophilic bacteria that have adapted to harsh environments by producing bioactive substances with remarkable properties. These molecules, including enzymes, peptides, and secondary metabolites, demonstrate exceptional stability, strong antioxidant properties, and advantageous biocompatibility, making them viable candidates for use in cosmeceuticals and nutraceuticals. This paper offers a thorough examination of the biotechnological potential of deep-sea extremophiles as a largely underutilised source of functional agents for cosmetics, anti-ageing products, and health-enhancing dietary ingredients. Key bioactive molecules, such as extremozymes, exopolysaccharides, and microbial pigments, along with their bioactivities, including ultraviolet protection, collagen stimulation, and oxidative stress attenuation, are examined, with an elucidation of their methods of action. Recent advancements in biotechnological instruments, particularly metagenomics and high-throughput screening, are analysed for their contributions to addressing the difficulties of producing extremophiles and aiding in the identification of new bioactive compounds. The paper examines existing limits in scalable bioprocessing and regulatory compliance, investigates solutions like synthetic biology and fermentation optimisation to enhance the viability of commercial utilisation. This study highlights the capacity of deep-sea extremophiles to considerably aid in the creation of sustainable, high-value goods in the health and wellness sectors.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA. 细小脲原体SMC蛋白与DNA相互作用的特性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf010

Natalia A Rumyantseva, Vladimir M Shutov, Dina G Belenkaia, Aleksandr A Alekseev, Natalia E Morozova, Alexey D Vedyaykin

引用次数: 0

Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency. 在浸泡过的食物垃圾中，曝气可以促进变形杆菌而不是厚壁菌，从而产生优越的厌氧消化效率。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf001

Linjie Tang, Mike Manefield

{"title":"Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency.","authors":"Linjie Tang, Mike Manefield","doi":"10.1093/femsle/fnaf001","DOIUrl":"10.1093/femsle/fnaf001","url":null,"abstract":"Aeration is a common pretreatment method to enhance biogas production via anaerobic digestion of waste organic feedstocks such as unused food. While impacts on downstream anaerobic digestion have been intensively investigated, the consequence of aeration on the microbial community in food waste has not been characterized. Food waste has a low pH resulting from the dominance of lactic acid bacteria within the Firmicutes phylum. This excludes other phylotypes with a higher potential to hydrolyse complex biopolymers in food waste. In this study, we reveal that aeration of macerated food waste results in a dramatic shift away from Firmicutes towards dominance of Proteobacteria that are better known for extracellular enzyme production. Given that hydrolysis is the rate limiting step in anaerobic digestion, this explains why aeration improves the efficiency of biogas production from food waste. The discovery that Proteobacteria dominate microbial communities in aerated food waste opens up opportunities to manipulate extracellular enzyme production through gene expression mechanisms common among Proteobacteria such as quorum sensing.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific postmortem microbiome dynamics in mice: implications for death definitions. 性别特异性小鼠死后微生物组动力学：对死亡定义的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf070

Yangkui Xue, Mengliang Huang, Jianing Zhang, Shrestha Navin, Yahao Tao, Gang Zeng

{"title":"Sex-specific postmortem microbiome dynamics in mice: implications for death definitions.","authors":"Yangkui Xue, Mengliang Huang, Jianing Zhang, Shrestha Navin, Yahao Tao, Gang Zeng","doi":"10.1093/femsle/fnaf070","DOIUrl":"10.1093/femsle/fnaf070","url":null,"abstract":"Gut microbes form a complex and dynamic symbiotic relationship with their host. However, the microbial response during the early stages following host death remains largely uncharacterized. In this study, we employed a mouse model to systematically characterize the postmortem response of the intestinal microbiota, and analyzed the dynamic changes in microbial composition during the early stages after death in both male and female mice (at 0, 0.5, 2, 6, 12, and 24 h postmortem). Our findings reveal that sex-dimorphic shifts in microbiome composition occur as early as 2 h postmortem. Male mice exhibited increased functional redundancy and delayed community restructuring, whereas female mice displayed earlier community shifts. These sex-specific patterns were accompanied by differences in metabolic pathway activity and biomarker taxa. Notably, the observed retention of regulatory capacity by intestinal microbes after host death offers a novel perspective on the conceptualization of death itself. We propose the term \"ecological death\" to describe the irreversible collapse of the host-associated microbial ecosystem following death, marking a critical transition in the functional and structural integrity of the intestinal microbiota.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0