Fems Microbiology Letters最新文献

Inhibitory activity of meso-dimercaptosuccinic acid against IMP metallo-β-lactamase variants in Pseudomonas aeruginosa. 中二巯基琥珀酸对铜绿假单胞菌IMP金属β-内酰胺酶变异的抑制作用。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-28 DOI: 10.1093/femsle/fnaf052

Jacqueline Findlay, Hector Moreno, Gilbert Greub, Christophe Le Terrier, Maxime Bouvier, Patrice Nordmann

{"title":"Inhibitory activity of meso-dimercaptosuccinic acid against IMP metallo-β-lactamase variants in Pseudomonas aeruginosa.","authors":"Jacqueline Findlay, Hector Moreno, Gilbert Greub, Christophe Le Terrier, Maxime Bouvier, Patrice Nordmann","doi":"10.1093/femsle/fnaf052","DOIUrl":"https://doi.org/10.1093/femsle/fnaf052","url":null,"abstract":"The treatment of infections caused by MBL-producing Pseudomonas aeruginosa (MBL-PA), including IMP-producers, can be challenging since MBLs confer resistance to most clinically available ß-lactams, leaving viable few options. Thiol-containing compounds, such as meso-dimercaptosuccinic acid (DMSA), have been suggested as inhibitors of MBLs, and particularly have been proposed to exert effective activity against IMP-1 enzymes. In this context, we sought to determine the activity of DMSA, in combination with carbapenems or cephalosporins, against diverse MBL and carbapenemase variants, particularly focussing on IMP variants. In recombinant strains producing either IMP-1, IMP-10 or IMP-13, both with and without a functional OprD, synergy was observed to ceftazidime (CAZ), cefepime (FEP) and meropenem. Negligible changes were observed for other MBLs and no effect was observed in stains producing KPC-2. Testing of the CAZ/DMSA and FEP/DMSA combinations against clinical IMP-producing P. aeruginosa isolates resulted in fractional inhibitory concentration indexes indicating synergy. Enzymatic assays showed the significantly increased inhibitory activity of DMSA against the IMP variants, compared to the other enzymes tested. These findings highlight a possible role of DMSA or DMSA-like compounds to be developed for the treatment of infections caused by IMP-producing P. aeruginosa.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surprising effects of differential loss in genome evolution: the last-one-out. 基因组进化中差异损失的惊人影响：最后一个出局。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-22 DOI: 10.1093/femsle/fnaf051

Nico Bremer, William F Martin, Mike Steel

引用次数: 0

Potential for microbial denitrification coupled with methanol oxidation found in abundant MAGs in Antarctic Peninsula sediments. 在南极半岛沉积物中发现丰富的MAGs中存在微生物反硝化和甲醇氧化的潜力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-21 DOI: 10.1093/femsle/fnaf050

Katie E Howland, Hannah J Nygaard, Andrew D Steen, Kenneth M Halanych, Andrew R Mahon, Deric R Learman

{"title":"Potential for microbial denitrification coupled with methanol oxidation found in abundant MAGs in Antarctic Peninsula sediments.","authors":"Katie E Howland, Hannah J Nygaard, Andrew D Steen, Kenneth M Halanych, Andrew R Mahon, Deric R Learman","doi":"10.1093/femsle/fnaf050","DOIUrl":"https://doi.org/10.1093/femsle/fnaf050","url":null,"abstract":"Denitrification accounts for a substantial nitrogen loss from environmental systems, shifting microbial composition and impacting other biogeochemical cycles. In Antarctica, rising temperatures cause increased organic matter deposition in marine sediments, which can significantly alter microbially mediated denitrification. To examine the genetic potential of microorganisms driving N-cycling in these sediments, benthic sediment cores were collected at two sites in the Weddell Sea, Antarctica. DNA was extracted from multiple depths at each site, resulting in the reconstruction of seventy-five high-quality metagenome-assembled genomes (MAGs). Forty-seven of these MAGs contained reductases involved in denitrification. MAGs belonging to the genus Methyloceanibacter were the most abundant MAGs at both sites and all depths, except depth 3-6 cmbsf at one site, where they were not identified. The abundance of these Methyloceanibacter MAGs suggests the potential for nitrate-driven methanol oxidation at both sites. MAGs belonging to Beggiatoaceae and Sedimenticolaceae were found to have the genetic potential to produce intermediates in denitrification and the complete pathway for dissimilatory nitrate reduction to ammonia (DNRA). MAGs within Acidimicrobiia and Dadabacteria had the potential to complete the final denitrification step. Based on MAGs, Antarctic peninsula sediment communities have the potential for complete denitrification and dissimilatory nitrate reduction to ammonia (DNRA) via a consortium.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T. regifaucium Amycolatopsis DSM 45072T编码decaplanin和kigamicin的NRPS和II型PKS生物合成基因簇的鉴定

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-19 DOI: 10.1093/femsle/fnaf049

Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur

{"title":"Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T.","authors":"Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur","doi":"10.1093/femsle/fnaf049","DOIUrl":"https://doi.org/10.1093/femsle/fnaf049","url":null,"abstract":"Amycolatopsis regifaucium, a Gram-positive actinomycete, is a prolific source of biologically active compounds, including polyphenol antibiotics like kigamicins. This study presents the draft genome of Amycolatopsis regifaucium DSM 45072T (= GY080T), which spans 8.28 Mbp and is assembled into 62 contigs, with annotation revealing 312 subsystems, 7,966 coding genes, and 52 RNAs, with a GC content of 68.5 mol%. We found a significant genomic diversity within the genus, revealing variations in core and accessory genomic elements across species. Multiple biosynthetic gene clusters (BGCs) have been identified, including a previously unidentified glycopeptide antibiotic (GPA) gene cluster and a type II polyketide synthase (PKS) gene cluster, highlighting the organism's metabolic versatility and potential for the biosynthesis of novel natural products. Our analysis confirmed the production of decaplanin, an antibiotic previously attributed to Amycolatopsis decaplanina DSM 44594T. Correspondingly, the gene cluster responsible for decaplanin biosynthesis is identified in A. regifaucium DSM 45072T and A. decaplanina DSM 44594T. Additionally, a putative type II PKS gene cluster is predicted within the glycopeptide antibiotic-producing clade (Cluster A) of the genus Amycolatopsis. Genomics insights from Amycolatopsis regifaucium DSM 45072T represent it as a promising genetic resource with significant implications for biotechnological and pharmaceutical innovation, particularly in discovering and developing novel antimicrobial agents.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces. 用于取样方法的消毒剂中和缓冲液对表面粘附单核增生李斯特菌细胞活力的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-19 DOI: 10.1093/femsle/fnaf048

Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet

{"title":"Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces.","authors":"Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet","doi":"10.1093/femsle/fnaf048","DOIUrl":"https://doi.org/10.1093/femsle/fnaf048","url":null,"abstract":"This study assessed the impact of six disinfectant-neutralizing buffers on Listeria monocytogenes adhering to stainless steel surfaces treated with quaternary ammonium or hydrogen peroxide-based disinfectants. The goal was to evaluate potential stressors induced by these buffers during sampling, minimizing false negatives in food industry surface monitoring. Neutralizing buffers are essential in preserving bacterial viability during sample transport by counteracting residual disinfectants. L. monocytogenes populations were quantified immediately after sampling and after 24-hour incubation at 8°C, simulating transport conditions. While neutralizing buffers had minimal impact on untreated adherent cells, they significantly reduced mortality in disinfectant-treated cells. However, most buffers failed to preserve viable culturable (VC) populations after disinfection, instead promoting viable but non-culturable (VBNC) states. Notably, prolonged incubation in the Sponge neutralizer facilitated VC population recovery, likely through VBNC resuscitation or VC growth. In contrast, other buffers inhibited recovery, suggesting detrimental effects on stressed cells due to their chemical composition. These findings underscore the importance of selecting appropriate neutralizing buffers in L. monocytogenes detection, influencing food safety surveillance and risk assessment protocols.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo - a pilot study. 微双孢菌素（NAI-107）在斑马鱼（Danio rerio）胚胎中的安全性初步评价。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-15 DOI: 10.1093/femsle/fnaf047

Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil

{"title":"Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo - a pilot study.","authors":"Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil","doi":"10.1093/femsle/fnaf047","DOIUrl":"https://doi.org/10.1093/femsle/fnaf047","url":null,"abstract":"NAI-107 has demonstrated in vitro and in vivo efficacy against multi drug resistance (MDR) pathogens. Studies using the Galleria mellonella model found NAI-107 effective against Neisseria gonorrhoeae and vanB-resistant Enterococcus faecium, with no toxicity at concentrations up to 64 µg/mL. This pilot study evaluated NAI-107's safety using transgenic zebrafish embryos (kdrl:eGFP) that were exposed to NAI-107 concentrations of 0, 10, 20, and 30 µM from 5 hours post-fertilization (hpf) to 4 days post-fertilization (dpf). No mortality or significant morphological abnormalities were observed across all treatment groups. At 2 dpf, larvae treated with 20 and 30 µM NAI-107 exhibited a significant increase in heart rate (p < 0.05), although other cardiac parameters remain unaffected. At 4 dpf, larvae displayed no significant growth differences but had increased locomotor activity at 20 and 30 µM NAI-107 groups, indicated by the higher total distance moved and mean velocity (p < 0.05). NAI-107, at concentrations up to 30 µM, did not induce significant toxic effects in zebrafish embryos. The absence of adverse impacts on survival or gross morphology suggests a favorable safety margin. However, the observed increases in heart rate and locomotor activity suggest possible subclinical effects at higher concentrations that warrant further investigation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara. 突尼斯撒哈拉沙漠南部两个地热绿洲嗜盐古菌群落的元分类分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae106

Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari

{"title":"Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara.","authors":"Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari","doi":"10.1093/femsle/fnae106","DOIUrl":"10.1093/femsle/fnae106","url":null,"abstract":"This study assesses halophilic archaea's phylogenetic diversity in southern Tunisia's geothermal water. In the arid southern regions, limited surface freshwater resources make geothermal waters a vital source for oases and greenhouse irrigation. Three samples, including water, sediment, and halite soil crust, were collected downstream of two geothermal springs of the Ksar Ghilane (KGH) and Zaouet Al Aness (ZAN) oases, Tunisia. The samples were subjected to 16S rRNA gene sequencing using the Illumina Miseq sequencing approach. Several haloarchaea were identified in the geothermal springs. The average taxonomic composition revealed that 20 out of 33 genera were shared between the two geothermal sources, with uneven distribution, where the Halogranum genus was the most represented genus with an abundance of 18.9% and 11.58% for ZAW and KGH, respectively. Several unique site-specific genera were observed: Halonotius, Halopelagius, Natronorubrum, and Haloarcula in ZAN, and Haloprofundus, Halomarina, Halovivax, Haloplanus, Natrinema, Halobium, Natronoarchaeum, and Haloterrigena in the KGH pool. Most genus members are typically found in low-salinity ecosystems. These findings suggest that haloarchaea can disperse downstream from geothermal sources and may survive temperature and chemical fluctuations in the runoff.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability. MEEhubs2024：以中心为基础的微生物生态学和进化促进可持续性会议。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf022

Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius

{"title":"MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability.","authors":"Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius","doi":"10.1093/femsle/fnaf022","DOIUrl":"10.1093/femsle/fnaf022","url":null,"abstract":"Scientific conferences are essential to academic exchange. However, related air travel contributes to greenhouse gas emissions, while expensive registration and travel costs limit the participation of early career researchers and those from low-income countries. Virtual conferences offer promising solutions for reducing emissions and enhancing accessibility and inclusivity but often limit networking and personal interaction. Hybrid multi-hub conferences, which combine virtually connected in-person venues with individual virtual participation, combine the benefits of both conference formats. Thus, we present and discuss MEEhubs2024, a multi-hub conference on microbial ecology and evolution held in January 2024. During this 3-day conference, attendees participated virtually or at one of six hubs in Europe and North America. We analyzed the participants' and organizers' feedback to create a template and provide insights into the scientific community's adoption of this new conference format, which was positively evaluated by most participants. Because technical, logistical, and structural challenges remain, including limited opportunities to interact and network across hubs and participation modes, we provide recommendations for improvement, such as hiring technical hosts and offering virtual-only social activities. Finally, we used the participants' feedback to reflect on conference expectations, highlighting research gaps and the need for organizers to define and communicate goals when organizing conferences.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency. 在浸泡过的食物垃圾中，曝气可以促进变形杆菌而不是厚壁菌，从而产生优越的厌氧消化效率。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf001

Linjie Tang, Mike Manefield

{"title":"Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency.","authors":"Linjie Tang, Mike Manefield","doi":"10.1093/femsle/fnaf001","DOIUrl":"10.1093/femsle/fnaf001","url":null,"abstract":"Aeration is a common pretreatment method to enhance biogas production via anaerobic digestion of waste organic feedstocks such as unused food. While impacts on downstream anaerobic digestion have been intensively investigated, the consequence of aeration on the microbial community in food waste has not been characterized. Food waste has a low pH resulting from the dominance of lactic acid bacteria within the Firmicutes phylum. This excludes other phylotypes with a higher potential to hydrolyse complex biopolymers in food waste. In this study, we reveal that aeration of macerated food waste results in a dramatic shift away from Firmicutes towards dominance of Proteobacteria that are better known for extracellular enzyme production. Given that hydrolysis is the rate limiting step in anaerobic digestion, this explains why aeration improves the efficiency of biogas production from food waste. The discovery that Proteobacteria dominate microbial communities in aerated food waste opens up opportunities to manipulate extracellular enzyme production through gene expression mechanisms common among Proteobacteria such as quorum sensing.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA. 细小脲原体SMC蛋白与DNA相互作用的特性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf010

Natalia A Rumyantseva, Vladimir M Shutov, Dina G Belenkaia, Aleksandr A Alekseev, Natalia E Morozova, Alexey D Vedyaykin

引用次数: 0