Fems Microbiology Letters最新文献

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About two French cases of disseminated Cryptococcus neoformans infection associated with COVID-19.
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-22 DOI: 10.1093/femsle/fnaf012
Wendy Pulby, Jérémy Lafolie, Chloé Belot, Loïc Dopeux, Sébastien Loiseau, Maxime Moniot, Philippe Poirier, Céline Nourrisson
{"title":"About two French cases of disseminated Cryptococcus neoformans infection associated with COVID-19.","authors":"Wendy Pulby, Jérémy Lafolie, Chloé Belot, Loïc Dopeux, Sébastien Loiseau, Maxime Moniot, Philippe Poirier, Céline Nourrisson","doi":"10.1093/femsle/fnaf012","DOIUrl":"https://doi.org/10.1093/femsle/fnaf012","url":null,"abstract":"<p><strong>Background: </strong>SARS-CoV-2 infection is an acute respiratory distress syndrome associated with immune dysfunction, causing COVID-19 disease. The use of immunosuppressive drugs in its treatment increases the risk of opportunistic infections. In particular, opportunistic fungal infections have been described in initially non-immunocompromised patients with severe COVID-19 disease. Among them, rare cases of cryptococcosis have been described.</p><p><strong>Case presentation: </strong>Here we present the first two French cases of non-HIV non-transplant patients who developed disseminated Cryptococcus neoformans fungal infection in the setting of severe COVID-19 disease.</p><p><strong>Conclusions: </strong>Blood cultures appear to be an interesting diagnostic tool for post-COVID-19 cryptococcosis, which is an often fatal complication.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro enzyme characterization and several inhibitors for monkeypox virus core protease I7L. 猴痘病毒核心蛋白酶I7L的体外酶学特性及几种抑制剂。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-17 DOI: 10.1093/femsle/fnaf008
Lin Wei, Yuqi Wu, Shuai Li, Jun Weng, Miaomiao Geng, Meng Mei, Zigong Wei
{"title":"In vitro enzyme characterization and several inhibitors for monkeypox virus core protease I7L.","authors":"Lin Wei, Yuqi Wu, Shuai Li, Jun Weng, Miaomiao Geng, Meng Mei, Zigong Wei","doi":"10.1093/femsle/fnaf008","DOIUrl":"https://doi.org/10.1093/femsle/fnaf008","url":null,"abstract":"<p><p>Monkeypox is a zoonotic viral disease caused by the monkeypox virus, a member of the genus Orthopoxvirus within the family Poxviridae, which also includes the variola virus. On Aug. 14, 2024, WHO Director-General declared monkeypox outbreak a public health emergency of international concern. Similar to variola virus core protease K7L, the I7L could be identified as a promising target to against monkeypox virus. Our work provides a solid foundation as well as specific molecular tools (protease production methods, assay design, inhibitor design) that can now be used to probe the function of I7L in vitro. Notably, in this work, various reported covalent lead compounds for COVID-19 proteases were screened and A68, shikonin and myricetin were identified as exhibiting high inhibitory activity against I7L. This work not only sheds light on effective inhibitors for the monkeypox virus core protease but also contributes to the broader search for antiviral agents targeting this enzyme.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Susceptibility and Genomic Characterization of Vibrio cholerae Non-O1/non-O139 Isolated from Clinical and Environmental Samples in Jiaxing City, China. 嘉兴市临床和环境中分离的非o1 /非o139型霍乱弧菌的药敏及基因组特征
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-17 DOI: 10.1093/femsle/fnaf009
Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen
{"title":"Antimicrobial Susceptibility and Genomic Characterization of Vibrio cholerae Non-O1/non-O139 Isolated from Clinical and Environmental Samples in Jiaxing City, China.","authors":"Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen","doi":"10.1093/femsle/fnaf009","DOIUrl":"https://doi.org/10.1093/femsle/fnaf009","url":null,"abstract":"<p><p>Non-O1/non-O139 (NOVC) strains inhabit aquatic environments and sporadically induce human illnesses. This study involved the virulence and antimicrobial genetic characterization of 176 NOVC strains, comprising 25 from clinical samples and 151 from environmental sources, collected between 2021 and 2023. The antimicrobial susceptibility of the examined NOVC population was predominantly high, exhibiting only poor susceptibility to colistin, with 89.2% resistance. The examination of virulence genes revealed that the majority of strains were positive for glucose metabolism (als gene) (169/176, 96.0%). Through multilocus sequence typing, the 176 NOVC strains were categorised into 121 sequence types, 79 of which were novel. NOVC strains demonstrate significant genetic variability and frequently engage in recombination. This work offers genetic characterization of the pathogenicity and antimicrobial resistance of a NOVC community. Our findings offer insights that may aid in the development of preventative and treatment methods for this pathogen.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998. 弯曲乳酸杆菌CP2998中脂磷胆酸的化学结构。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-15 DOI: 10.1093/femsle/fnaf005
Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota
{"title":"Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998.","authors":"Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota","doi":"10.1093/femsle/fnaf005","DOIUrl":"https://doi.org/10.1093/femsle/fnaf005","url":null,"abstract":"<p><p>Latilactobacillus curvatus, found in various fermented foods, is a promising probiotic with unique health benefits. Lipoteichoic acid (LTA) is a characteristic amphiphilic surface polymer of gram-positive bacteria and exhibits immunomodulatory activities. Despite the structural diversity of LTA among different bacterial species and strains, no information is available on the chemical structure of LTA in L. curvatus. In this study, we aimed to determine the structure of LTA isolated from L. curvatus CP2998. One- and two-dimensional nuclear magnetic resonance spectra of intact LTA revealed that LTA had a glycerolphosphate polymer as a hydrophilic main chain with partial substitutions of α-linked glucose and D-alanine at the hydroxy group at position 2 of the glycerol residue. The anchor glycolipid fraction was obtained by hydrofluoric acid treatment. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry spectrum of the anchor glycolipid revealed that it contained diglucosyldiacylglycerol and diglucosylmonoacylglycerol. Our results suggest that L. curvatus CP2998 possesses a typical type I LTA structure; however, the lactic acid bacteria-specific anchor glycolipid structures, such as tri- or tetra-saccharides and three fatty acid residues, were not identified.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring 1-alkene biosynthesis in bacterial antagonists and jeotgalicoccus sp. ATCC 8456. 1-烯烃生物合成在细菌拮抗剂和焦糖球菌(jeotgaliccoccus sp. ATCC 8456)中的应用
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-13 DOI: 10.1093/femsle/fnaf004
Matthias Schweitzer, Andrea Marianne Friedrich, Alexander Dennig, Gabriele Berg, Christina Andrea Müller Bogotá
{"title":"Exploring 1-alkene biosynthesis in bacterial antagonists and jeotgalicoccus sp. ATCC 8456.","authors":"Matthias Schweitzer, Andrea Marianne Friedrich, Alexander Dennig, Gabriele Berg, Christina Andrea Müller Bogotá","doi":"10.1093/femsle/fnaf004","DOIUrl":"https://doi.org/10.1093/femsle/fnaf004","url":null,"abstract":"<p><p>Terminal olefins are important platform chemicals, drop-in compatible hydrocarbons and also play an important role as biocontrol agents of plant pathogens. Currently, 1-alkenes are derived from petroleum, although microbial biosynthetic routes are known. Jeotgalicoccus sp. ATCC 8456 produces 1-alkenes via the fatty acid decarboxylase OleTJE. UndA and UndB are recently identified non-heme iron oxidases converting medium-chain fatty acids into terminal alkenes. Our knowledge about the diversity and natural function of OleTJE, UndA and UndB homologs is scarce. We applied a combined screening strategy-SPME GC-MS and PCR-based amplification-to survey an environmental strain collection for microbial 1-alkene producers and their corresponding enzymes. Our results reinforce the high level of conservation of UndA and UndB genes across the genus Pseudomonas. In vivo production of defined 1-alkenes (C9-C13; C15; C19) was directed by targeted feeding of fatty acids. Lauric acid feeding enabled 1-undecene production to a concentration of 3.05 mg L-1 in Jeotgalicoccus sp. ATCC 8456 and enhanced its production by 105% in Pseudomonas putida 1T1 (1.10 mg L-1). Besides, whole genome sequencing of Jeotgalicoccus sp. ATCC 8456 enabled reconstruction of the 1-alkene biosynthetic pathway. These results advance our understanding of microbial 1-alkene synthesis and the underlying genetic basis.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria. 一种来自山地湿地的嗜中性、降解木聚糖的植物菌——石勒斯里亚。石勒斯里亚属的修正描述。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-13 DOI: 10.1093/femsle/fnaf006
Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh
{"title":"Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria.","authors":"Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh","doi":"10.1093/femsle/fnaf006","DOIUrl":"https://doi.org/10.1093/femsle/fnaf006","url":null,"abstract":"<p><p>Aerobic chemoorganotrophic planctomycetes of the genus Schlesneria colonize a wide spectrum of freshwater ecosystems. The only described species of this genus, S. paludicola, is represented by ellipsoid-shaped, moderately acidophilic bacteria isolated from acidic peat bogs. Here, we characterize a novel neutrophilic member of this genus from a mountain wetland, strain T3-172T. This isolate possesses unpigmented, non-motile, spherical cells, which grow on media containing some mono- and polysaccharides in the pH range of 6.0-7.5 and at temperatures between 15 and 37°C. Good growth is observed on xylan, one of the major plant cell wall biopolymers. The genome of strain T3-172T is 7.16 Mb in size and contains two unlinked sets of rRNA genes, 93 tRNA genes, and about 5500 potential protein-coding genes. Among the latter, the GH5 family protein is identified as the most reliable candidate for the cleavage of xylan. The 16S rRNA gene sequence of strain T3-172T displays 97.8% similarity to that of S. paludicola MPL7T, and the average nucleotide identity between the genomes of these planctomycetes is 71.6%. We, therefore, propose a novel species of the genus Schlesneria, S. sphaerica sp. nov., with strain T3-172T (=KCTC 102306T = VKM B-3856T) as the type strain.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity. 位于VIM-2活性位点附近的保守辅助残基影响其金属β-内酰胺酶活性。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-11 DOI: 10.1093/femsle/fnaf007
Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh
{"title":"Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity.","authors":"Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh","doi":"10.1093/femsle/fnaf007","DOIUrl":"https://doi.org/10.1093/femsle/fnaf007","url":null,"abstract":"<p><p>Verona-integron-metallo-β-lactamase (VIM-2) is one of the most widespread class B β-lactamase responsible for β-lactam resistance. Although active-site residues help in metal binding, the residues nearing the active-site possess functional importance. Here, to decipher the role of such residues in the activity and stability of VIM-2, the residues E146, D182, N210, S207, and D213 were selected through in-silico analyses and substituted with alanine using site-directed mutagenesis. The effects of substitution mutations were assessed by comparing the changes in β-lactam susceptibility pattern of E. coli host cell expressing VIM-2 and its mutated proteins. VIM-2_N210A enhanced the susceptibility of the host by ∼4-8 folds against penicillins and cephalosporins while the expression of VIM-2_D182A radically increased the susceptibility of host. However, expression of VIM-2_E146A reduced the susceptibility of host by 2-fold. Further, proteins were purified to homogeneity, and VIM_N210A and VIM_D182A displayed reduced thermal stability than VIM-2. Moreover, in vitro catalytic efficiencies of VIM-2_D182A were drastically reduced against all the β-lactams tested whereas the same were moderately reduced for VIM-2_N210A. Conversely, the catalytic efficiency was marginally altered for VIM_E146A. Overall, we infer that both N210A and D182A substitutions negatively affect the performance of VIM-2 by influencing substrate specificity and stability, respectively.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara. 突尼斯撒哈拉沙漠南部两个地热绿洲嗜盐古菌群落的元分类分析。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae106
Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari
{"title":"Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara.","authors":"Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari","doi":"10.1093/femsle/fnae106","DOIUrl":"10.1093/femsle/fnae106","url":null,"abstract":"<p><p>This study assesses halophilic archaea's phylogenetic diversity in southern Tunisia's geothermal water. In the arid southern regions, limited surface freshwater resources make geothermal waters a vital source for oases and greenhouse irrigation. Three samples, including water, sediment, and halite soil crust, were collected downstream of two geothermal springs of the Ksar Ghilane (KGH) and Zaouet Al Aness (ZAN) oases, Tunisia. The samples were subjected to 16S rRNA gene sequencing using the Illumina Miseq sequencing approach. Several haloarchaea were identified in the geothermal springs. The average taxonomic composition revealed that 20 out of 33 genera were shared between the two geothermal sources, with uneven distribution, where the Halogranum genus was the most represented genus with an abundance of 18.9% and 11.58% for ZAW and KGH, respectively. Several unique site-specific genera were observed: Halonotius, Halopelagius, Natronorubrum, and Haloarcula in ZAN, and Haloprofundus, Halomarina, Halovivax, Haloplanus, Natrinema, Halobium, Natronoarchaeum, and Haloterrigena in the KGH pool. Most genus members are typically found in low-salinity ecosystems. These findings suggest that haloarchaea can disperse downstream from geothermal sources and may survive temperature and chemical fluctuations in the runoff.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency. 在浸泡过的食物垃圾中,曝气可以促进变形杆菌而不是厚壁菌,从而产生优越的厌氧消化效率。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf001
Linjie Tang, Mike Manefield
{"title":"Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency.","authors":"Linjie Tang, Mike Manefield","doi":"10.1093/femsle/fnaf001","DOIUrl":"10.1093/femsle/fnaf001","url":null,"abstract":"<p><p>Aeration is a common pretreatment method to enhance biogas production via anaerobic digestion of waste organic feedstocks such as unused food. While impacts on downstream anaerobic digestion have been intensively investigated, the consequence of aeration on the microbial community in food waste has not been characterized. Food waste has a low pH resulting from the dominance of lactic acid bacteria within the Firmicutes phylum. This excludes other phylotypes with a higher potential to hydrolyse complex biopolymers in food waste. In this study, we reveal that aeration of macerated food waste results in a dramatic shift away from Firmicutes towards dominance of Proteobacteria that are better known for extracellular enzyme production. Given that hydrolysis is the rate limiting step in anaerobic digestion, this explains why aeration improves the efficiency of biogas production from food waste. The discovery that Proteobacteria dominate microbial communities in aerated food waste opens up opportunities to manipulate extracellular enzyme production through gene expression mechanisms common among Proteobacteria such as quorum sensing.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples. 菲达干酪植物乳杆菌细菌素基因及抑菌活性测定。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf002
Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel
{"title":"Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples.","authors":"Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel","doi":"10.1093/femsle/fnaf002","DOIUrl":"10.1093/femsle/fnaf002","url":null,"abstract":"<p><p>In this study designed to isolate lactic acid bacteria (LAB) with bacteriocin production potential, white cheese samples were collected from different provinces of Turkey and isolation was carried out. A series of experiments were carried out for the main purpose and the actual bacteriocin producers were identified by detecting the genes encoding this bacteriocin. The experiments carried out in this direction were initially carried out with 20 isolates and as a result of various experiments, the number of isolates was reduced to 8 and the study was continued with 8 isolates. In order to determine that the eight isolates identified as a result of a phenotypic and biochemical characterization study were true bacteriocin-producing strains, their antibacterial activity was investigated and then the presence of bacteriocin genes was examined by specific polymerase chain reaction (PCR) using gene-specific primers. As a result, MS16 coded Lactiplantibacillus plantarum OR922652 was found to have strong antibacterial activity against Escherichia coli, Klebsiella pneumonia, Yersinia enterocolitica, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus; the isolate was susceptible to clinically important antibiotics (ciprofloxacin, gentamicin, penicillin G, ampicillin, chloramphenicol, and vancomycin) and resistant to erythromycin, had no hemolytic activity and possessed plnA and plnD genes encoding bacteriocin production. In conclusion, the MS16 coded L. plantarum isolate has emerged as a promising strain that can be used especially in the health field and in the food industry related to LAB.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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