Fems Microbiology Letters最新文献

Purification of Active Staphylococcal Alpha-Toxin produced in Bacillus subtilis. 枯草芽孢杆菌产生的活性葡萄球菌α毒素的纯化。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-07-17 DOI: 10.1093/femsle/fnaf073

Hoa T T Vo, Dat D Truong, Danh V Bui, Hoang T Le, Luyen T Vu, Dinh T M Tran, Diep T M Nguyen, Hoang D Nguyen

{"title":"Purification of Active Staphylococcal Alpha-Toxin produced in Bacillus subtilis.","authors":"Hoa T T Vo, Dat D Truong, Danh V Bui, Hoang T Le, Luyen T Vu, Dinh T M Tran, Diep T M Nguyen, Hoang D Nguyen","doi":"10.1093/femsle/fnaf073","DOIUrl":"https://doi.org/10.1093/femsle/fnaf073","url":null,"abstract":"The Gram-positive bacterium Staphylococcus aureus, a multidrug-resistant pathogen, poses a significant threat to human and animal health. Its virulence is largely attributed to the production of Alpha-Toxin (Hla), a potent cytotoxic protein that forms pores in cell membranes, leading to lysis. This is the first study to report the successful expression and purification of wild-type Hla (HlaWT) using Bacillus subtilis-a safe, genetically stable, and endotoxin-free host system. A vector was constructed harboring the hla gene fused to Strep-tag II at the C-terminus under the control of a strong IPTG-inducible Pgrac212 promoter in a pHT expression vector for B. subtilis. Following IPTG induction, extracellular Hla was analyzed by SDS-PAGE and confirmed by Western blotting using anti-Hla polyclonal antibodies. The Hla protein was purified in a single step using a Strep-Tactin column and showed a high response to the antibodies, as confirmed by ELISA. Hemolytic measurements using rabbit red blood cells demonstrated that Hla exhibits potent hemolytic activity. These findings demonstrate the potential of B. subtilis as an effective host for producing biologically active extracellular toxins and provide a scalable, safe platform for HlaWT production in pathogenesis research, diagnostics, immunology, drug screening, and toxoid vaccine development.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linking Plastic Degradation Potential and Resistance Gene Abundance in Bacterioplankton Community of the Sundarbans Estuarine Ecosystem. 孙德尔本斯河口生态系统浮游细菌群落塑料降解潜力与抗性基因丰度的关联

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-07-04 DOI: 10.1093/femsle/fnaf067

Nirupama Saini, Anwesha Ghosh, Punyasloke Bhadury

{"title":"Linking Plastic Degradation Potential and Resistance Gene Abundance in Bacterioplankton Community of the Sundarbans Estuarine Ecosystem.","authors":"Nirupama Saini, Anwesha Ghosh, Punyasloke Bhadury","doi":"10.1093/femsle/fnaf067","DOIUrl":"https://doi.org/10.1093/femsle/fnaf067","url":null,"abstract":"Harnessing microbial capabilities offers a promising and sustainable approach to address the global challenge of plastic waste. However, the potential of mangrove microbiomes to degrade diverse plastic polymers remains largely unexplored. In this metagenomic-based study, surface water microbiomes were analysed from the Indian Sundarbans, part of the world's largest contiguous mangrove ecosystem, revealing 748.21 hits per billion nucleotides associated with plastic-degrading enzymes (PDEs) targeting 17 different polymer types. Of these, 72.9% corresponded to synthetic polymers and 27.1% to natural polymers. The highest number of hits (223) was associated with polyethylene glycol-degrading enzymes, representing 26.7% of the total PDEs hits. Taxonomic analysis revealed Deltaproteobacteria and Gammaproteobacteria as key degraders of diverse synthetic plastic polymers, with Deltaproteobacteria emerging as a previously unreported group. This suggests that surface sediments may serve as reservoirs for novel plastic-degrading microbes. Co-occurrence network analysis indicated possible emerging co-selection or complex associations between PDEs, antibiotic resistance genes (ARGs), and metal resistance genes (MRGs). Notably, zinc resistance genes and aminoglycoside-related ARGs showed more associations with PDEs. While the presence of PDEs offers a promising avenue for bioremediation, their application may be complicated by the concurrent rise of ARGs and MRGs within PDE-harbouring microbes. Thus, it highlights the need for careful assessment when employing microbes for plastic bioremediation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability. MEEhubs2024：以中心为基础的微生物生态学和进化促进可持续性会议。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf022

Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius

{"title":"MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability.","authors":"Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius","doi":"10.1093/femsle/fnaf022","DOIUrl":"10.1093/femsle/fnaf022","url":null,"abstract":"Scientific conferences are essential to academic exchange. However, related air travel contributes to greenhouse gas emissions, while expensive registration and travel costs limit the participation of early career researchers and those from low-income countries. Virtual conferences offer promising solutions for reducing emissions and enhancing accessibility and inclusivity but often limit networking and personal interaction. Hybrid multi-hub conferences, which combine virtually connected in-person venues with individual virtual participation, combine the benefits of both conference formats. Thus, we present and discuss MEEhubs2024, a multi-hub conference on microbial ecology and evolution held in January 2024. During this 3-day conference, attendees participated virtually or at one of six hubs in Europe and North America. We analyzed the participants' and organizers' feedback to create a template and provide insights into the scientific community's adoption of this new conference format, which was positively evaluated by most participants. Because technical, logistical, and structural challenges remain, including limited opportunities to interact and network across hubs and participation modes, we provide recommendations for improvement, such as hiring technical hosts and offering virtual-only social activities. Finally, we used the participants' feedback to reflect on conference expectations, highlighting research gaps and the need for organizers to define and communicate goals when organizing conferences.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency. 在浸泡过的食物垃圾中，曝气可以促进变形杆菌而不是厚壁菌，从而产生优越的厌氧消化效率。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf001

Linjie Tang, Mike Manefield

{"title":"Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency.","authors":"Linjie Tang, Mike Manefield","doi":"10.1093/femsle/fnaf001","DOIUrl":"10.1093/femsle/fnaf001","url":null,"abstract":"Aeration is a common pretreatment method to enhance biogas production via anaerobic digestion of waste organic feedstocks such as unused food. While impacts on downstream anaerobic digestion have been intensively investigated, the consequence of aeration on the microbial community in food waste has not been characterized. Food waste has a low pH resulting from the dominance of lactic acid bacteria within the Firmicutes phylum. This excludes other phylotypes with a higher potential to hydrolyse complex biopolymers in food waste. In this study, we reveal that aeration of macerated food waste results in a dramatic shift away from Firmicutes towards dominance of Proteobacteria that are better known for extracellular enzyme production. Given that hydrolysis is the rate limiting step in anaerobic digestion, this explains why aeration improves the efficiency of biogas production from food waste. The discovery that Proteobacteria dominate microbial communities in aerated food waste opens up opportunities to manipulate extracellular enzyme production through gene expression mechanisms common among Proteobacteria such as quorum sensing.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evolution of ciprofloxacin resistance in Neisseria commensals and derived mutation population dynamics in natural Neisseria populations. 共生奈瑟菌环丙沙星耐药性体外进化及自然奈瑟菌群体衍生突变种群动态。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae107

Leah R Robinson, Caroline J McDevitt, Molly R Regan, Sophie L Quail, Crista B Wadsworth

{"title":"In vitro evolution of ciprofloxacin resistance in Neisseria commensals and derived mutation population dynamics in natural Neisseria populations.","authors":"Leah R Robinson, Caroline J McDevitt, Molly R Regan, Sophie L Quail, Crista B Wadsworth","doi":"10.1093/femsle/fnae107","DOIUrl":"10.1093/femsle/fnae107","url":null,"abstract":"Commensal Neisseria are members of a healthy human oropharyngeal microbiome; however, they also serve as a reservoir of antimicrobial resistance for their pathogenic relatives. Despite their known importance as sources of novel genetic variation for pathogens, we still do not understand the full suite of resistance mutations commensal species can harbor. Here, we use in vitro selection to assess the mutations that emerge in response to ciprofloxacin selection in commensal Neisseria by passaging four replicates of four different species in the presence of a selective antibiotic gradient for 20 days; then categorized derived mutations with whole genome sequencing. Ten out of sixteen selected cells lines across the four species evolved ciprofloxacin resistance (≥1 ug/ml); with resistance-contributing mutations primarily emerging in DNA gyrase subunit A and B (gyrA and gyrB), topoisomerase IV subunits C and E (parC and parE), and the multiple transferable efflux pump repressor (mtrR). Of note, these derived mutations appeared in the same loci responsible for ciprofloxacin-reduced susceptibility in the pathogenic Neisseria, suggesting conserved mechanisms of resistance across the genus. Additionally, we tested for zoliflodacin cross-resistance in evolved strain lines and found 6 lineages with elevated zoliflodacin minimum inhibitory concentrations. Finally, to interrogate the likelihood of experimentally derived mutations emerging and contributing to resistance in natural Neisseria, we used a population-based approach and identified GyrA 91I as a substitution circulating within commensal Neisseria populations and ParC 85C in a single gonococcal isolate. A small cluster of gonococcal isolates shared commensal alleles at parE, suggesting recent cross-species recombination events.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11774118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly synthesis of silver nanoparticles using lignin peroxidase produced from Caldibacillus thermoamylovorans and cultivated on waste walnut shell. 利用热淀粉样芽孢杆菌产生的木质素过氧化物酶在废核桃壳上的生态合成纳米银。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf036

Sefa Nur Akkaya, Ammar Almansour, Mehmet Akif Omeroglu, Hayrunnisa Nadaroglu, Ahmet Adiguzel

{"title":"Eco-friendly synthesis of silver nanoparticles using lignin peroxidase produced from Caldibacillus thermoamylovorans and cultivated on waste walnut shell.","authors":"Sefa Nur Akkaya, Ammar Almansour, Mehmet Akif Omeroglu, Hayrunnisa Nadaroglu, Ahmet Adiguzel","doi":"10.1093/femsle/fnaf036","DOIUrl":"10.1093/femsle/fnaf036","url":null,"abstract":"The preparation of silver nanoparticles (AgNPs) via an environmentally friendly green synthesis method represents an ecologically promising alternative. This research aims to develop sustainable and eco-friendly AgNPs using the lignin peroxidase (LiP) enzyme from Caldibacillus thermoamylovorans, cultivated on waste walnut shells, which are rich in lignin, to meet the growing demand for AgNPs. Among thermophilic bacteria that were isolated, the C. thermoamylovorans SA1 strain showed the highest LiP activity. The production of LiP was optimized by adding waste walnut shells and manipulating the environmental parameters. The optimal conditions were determined at 50 g/l shell amount, 96 h, pH 8, 140 rpm, and 60°C. In parallel with the increase in enzyme activity, bacterial growth also increased. As a result of the optimization, the highest enzyme activity value was 435.0 U/ml and bacterial growth was determined to be OD600: 2.09. The extracellular medium obtained from the bacteria grown in walnut shell medium was then added to an AgNO3 solution. Efficient production of AgNPs was achieved by stirring the mixture at 50°C-60°C for 4 h under optimum conditions. The synthesized AgNPs were characterized using a range of analytical techniques, including UV‒Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The biological efficacy of the synthesized AgNPs was evaluated by assessing their antibacterial activity against pathogenic bacteria, such as Escherichia coli O157:H7, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, and Bacillus cereus. The highest activity was observed against B. cereus (15 mm). The broad-spectrum antibacterial properties exhibited by the AgNPs synthesized in this study offer a promising and sustainable solution for diverse applications in various sectors, including the environmental, agricultural, medical, and pharmaceutical fields.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria. 植物酚类化合物对氨基酸发酵菌产氨的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf018

Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe

{"title":"Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria.","authors":"Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe","doi":"10.1093/femsle/fnaf018","DOIUrl":"10.1093/femsle/fnaf018","url":null,"abstract":"Bacteria that ferment amino acids to ammonia can be categorized as generalists or specialist hyper-ammonia-producing bacteria. In the rumens of ruminant animals, most of the ammonia produced is eventually excreted as urea in urine. This process can be controlled with off-label use of antibiotics, but the practice can lead to antibiotic resistance; therefore, discovery of antibiotic alternatives is pertinent. Plant-derived phenolic compounds have demonstrated antimicrobial efficacy for such purposes. This study investigated the antimicrobial and metabolic suppressive potential of six phenolic compounds on five amino acid fermenting bacteria: Clostridium sporogenes MD1, C. aminophilum F, Acetoanaerobium sticklandii SR, Peptostreptococcus sp. BG1, and Prevotella bryantii B14. Inhibitory action of the compounds was determined using a 10% v/v serial dilution method in basal media. Carvacrol (1 mM), thymol (1 mM), and eugenol (10 mM) demonstrated the greatest antimicrobial potential, where carvacrol and eugenol inhibited growth of all five species and thymol four species except BG1. The cinnamic acids (trans and hydro) demonstrated variable activity against all organisms. Suppression of metabolic activity was determined via colorimetric assay quantifying ammonia in washed stationary phase culture supernatant after 24 h of metabolism on fresh substrate. Carvacrol and eugenol yielded the greatest reduction of ammonia by all organisms except B14, which produced no ammonia under the growth conditions. Thymol greatly reduced ammonia production of four organisms except F. These data demonstrate that eugenol, carvacrol, and thymol may be worthy antimicrobial candidates for the control of ammonia-producing organisms.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara. 突尼斯撒哈拉沙漠南部两个地热绿洲嗜盐古菌群落的元分类分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae106

Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari

{"title":"Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara.","authors":"Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari","doi":"10.1093/femsle/fnae106","DOIUrl":"10.1093/femsle/fnae106","url":null,"abstract":"This study assesses halophilic archaea's phylogenetic diversity in southern Tunisia's geothermal water. In the arid southern regions, limited surface freshwater resources make geothermal waters a vital source for oases and greenhouse irrigation. Three samples, including water, sediment, and halite soil crust, were collected downstream of two geothermal springs of the Ksar Ghilane (KGH) and Zaouet Al Aness (ZAN) oases, Tunisia. The samples were subjected to 16S rRNA gene sequencing using the Illumina Miseq sequencing approach. Several haloarchaea were identified in the geothermal springs. The average taxonomic composition revealed that 20 out of 33 genera were shared between the two geothermal sources, with uneven distribution, where the Halogranum genus was the most represented genus with an abundance of 18.9% and 11.58% for ZAW and KGH, respectively. Several unique site-specific genera were observed: Halonotius, Halopelagius, Natronorubrum, and Haloarcula in ZAN, and Haloprofundus, Halomarina, Halovivax, Haloplanus, Natrinema, Halobium, Natronoarchaeum, and Haloterrigena in the KGH pool. Most genus members are typically found in low-salinity ecosystems. These findings suggest that haloarchaea can disperse downstream from geothermal sources and may survive temperature and chemical fluctuations in the runoff.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring 1-alkene biosynthesis in bacterial antagonists and Jeotgalicoccus sp. ATCC 8456. 1-烯烃生物合成在细菌拮抗剂和焦糖球菌（jeotgaliccoccus sp. ATCC 8456）中的应用

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf004

Matthias Schweitzer, Andrea Marianne Friedrich, Alexander Dennig, Gabriele Berg, Christina Andrea Müller Bogotá

{"title":"Exploring 1-alkene biosynthesis in bacterial antagonists and Jeotgalicoccus sp. ATCC 8456.","authors":"Matthias Schweitzer, Andrea Marianne Friedrich, Alexander Dennig, Gabriele Berg, Christina Andrea Müller Bogotá","doi":"10.1093/femsle/fnaf004","DOIUrl":"10.1093/femsle/fnaf004","url":null,"abstract":"Terminal olefins are important platform chemicals, drop-in compatible hydrocarbons and also play an important role as biocontrol agents of plant pathogens. Currently, 1-alkenes are derived from petroleum, although microbial biosynthetic routes are known. Jeotgalicoccus sp. ATCC 8456 produces 1-alkenes via the fatty acid decarboxylase OleTJE. UndA and UndB are recently identified non-heme iron oxidases converting medium-chain fatty acids into terminal alkenes. Our knowledge about the diversity and natural function of OleTJE, UndA, and UndB homologs is scarce. We applied a combined screening strategy-solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME GC-MS) and polymerase chain reaction (PCR)-based amplification-to survey an environmental strain collection for microbial 1-alkene producers and their corresponding enzymes. Our results reinforce the high level of conservation of UndA and UndB genes across the genus Pseudomonas. In vivo production of defined 1-alkenes (C9-C13; C15; C19) was directed by targeted feeding of fatty acids. Lauric acid feeding enabled 1-undecene production to a concentration of 3.05 mg l-1 in Jeotgalicoccus sp. ATCC 8456 and enhanced its production by 105% in Pseudomonas putida 1T1 (1.10 mg l-1). Besides, whole genome sequencing of Jeotgalicoccus sp. ATCC 8456 enabled reconstruction of the 1-alkene biosynthetic pathway. These results advance our understanding of microbial 1-alkene synthesis and the underlying genetic basis.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-specific postmortem microbiome dynamics in mice: implications for death definitions. 性别特异性小鼠死后微生物组动力学：对死亡定义的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf070

Yangkui Xue, Mengliang Huang, Jianing Zhang, Shrestha Navin, Yahao Tao, Gang Zeng

{"title":"Sex-specific postmortem microbiome dynamics in mice: implications for death definitions.","authors":"Yangkui Xue, Mengliang Huang, Jianing Zhang, Shrestha Navin, Yahao Tao, Gang Zeng","doi":"10.1093/femsle/fnaf070","DOIUrl":"10.1093/femsle/fnaf070","url":null,"abstract":"Gut microbes form a complex and dynamic symbiotic relationship with their host. However, the microbial response during the early stages following host death remains largely uncharacterized. In this study, we employed a mouse model to systematically characterize the postmortem response of the intestinal microbiota, and analyzed the dynamic changes in microbial composition during the early stages after death in both male and female mice (at 0, 0.5, 2, 6, 12, and 24 h postmortem). Our findings reveal that sex-dimorphic shifts in microbiome composition occur as early as 2 h postmortem. Male mice exhibited increased functional redundancy and delayed community restructuring, whereas female mice displayed earlier community shifts. These sex-specific patterns were accompanied by differences in metabolic pathway activity and biomarker taxa. Notably, the observed retention of regulatory capacity by intestinal microbes after host death offers a novel perspective on the conceptualization of death itself. We propose the term \"ecological death\" to describe the irreversible collapse of the host-associated microbial ecosystem following death, marking a critical transition in the functional and structural integrity of the intestinal microbiota.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0