Fems Microbiology Letters最新文献

Improving the thermal tolerance of biocontrol spores, Akanthomyces lecanii, by encapsulation. 用包封法提高生防孢子的耐热性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-06-16 DOI: 10.1093/femsle/fnaf062

Paul W Baker, Ephraim Nuwamanya, Adam Charlton

{"title":"Improving the thermal tolerance of biocontrol spores, Akanthomyces lecanii, by encapsulation.","authors":"Paul W Baker, Ephraim Nuwamanya, Adam Charlton","doi":"10.1093/femsle/fnaf062","DOIUrl":"https://doi.org/10.1093/femsle/fnaf062","url":null,"abstract":"Akanthomyces lecanii is an entomopathogenic fungus, and spores of this fungus could be incorporated into films generated using cast film extrusion for biocontrol applications. However, the extrusion process involves high temperature processing (150°C) although this only lasts for a few minutes. The elevated temperature destroys spores, thereby eliminating functionality, unless the spores are protected from this heat. Initial experiments revealed that the heat tolerance of free A. lecanii spores to be 60°C. The spores were therefore encapsulated into beads prepared using a combination of Gelrite, cellulose and Cel-fine at different concentrations. The beads were freeze-dried and then immersed in hot glycerol for 2 minutes at a selected temperature within the range of 50-100°C. The results indicated that some combinations of encapsulating agents resulted in the spores retaining viability (plate counting) after heat treatment at 100°C. Beads stored at room temperature for one week showed a reduction in the upper temperature tolerance. This study revealed that the temperature tolerance of A. lecanii spores could be improved by 40°C by encapsulation in freeze-dried beads containing 2% Gelrite (purified gellum gum), 0.4% cellulose and 0.4% Cel-fine.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroMundo@Oeiras: Citizen Science promoting antibiotic stewardship, discovery of new antimicrobials and monitoring of soil resistance. MicroMundo@Oeiras：公民科学促进抗生素管理，发现新的抗菌剂和监测土壤耐药性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-06-13 DOI: 10.1093/femsle/fnaf061

Bruna Henriques, Bruna Brito, Catarina Barreiros, Guilherme Galvão, João Coito, Sofia Lourinho, Teresa Mil-Homens, Verónica Maciel, Afonso Queijinho, Beatriz Cardoso, Leonor Maria, Mariyana Vatova, Rodrigo Bastos, Sara Carrera Prata, Lígia O Martins, Renata Ramalho, Ana Silva, Elisabete Brigadeiro, Maria João Leão, Pedro Matos Pereira, Paulo Durão

{"title":"MicroMundo@Oeiras: Citizen Science promoting antibiotic stewardship, discovery of new antimicrobials and monitoring of soil resistance.","authors":"Bruna Henriques, Bruna Brito, Catarina Barreiros, Guilherme Galvão, João Coito, Sofia Lourinho, Teresa Mil-Homens, Verónica Maciel, Afonso Queijinho, Beatriz Cardoso, Leonor Maria, Mariyana Vatova, Rodrigo Bastos, Sara Carrera Prata, Lígia O Martins, Renata Ramalho, Ana Silva, Elisabete Brigadeiro, Maria João Leão, Pedro Matos Pereira, Paulo Durão","doi":"10.1093/femsle/fnaf061","DOIUrl":"https://doi.org/10.1093/femsle/fnaf061","url":null,"abstract":"The alarming rise of antimicrobial resistance (AMR) is an emerging public health challenge to our society. Although resistance is an inevitable evolutionary process, finding new antimicrobials, mapping antimicrobial resistance, and raising community awareness leading to better hygiene attitudes and more responsible antibiotic consumption, when treatment is really required, is a cost-effective route to slow down the pace of resistance propagation. In MicroMundo@Oeiras, a citizen science project implemented at the ITQB NOVA research institute, we have successfully expanded to the Oeiras Municipality in the Lisbon metropolitan area (Portugal) the service-learning MicroMundo project based on the Tiny Earth / Small World Initiative, aimed to explore soil microbial biodiversity in search for novel antibiotics and simultaneously educating a fraction of the community at the university, and junior and high school level about antimicrobial resistance. Moreover, we have successfully extended the MicroMundo protocols to characterize the frequency of resistant soil bacteria to the antibiotics' amoxicillin, tetracycline, and ciprofloxacin. Our preliminary results using this coupled analysis suggest that AMR soil bacteria are likely to be more prevalent than antibiotic producing bacteria, creating a stimulating experimental outcome for the enrolled citizen scientists, and broadening the scope of possible scientific questions. We have also created a public database where all AMR frequency found in the isolated soil bacteria will be regularly updated, creating a novel AMR data source with information from unconventional monitoring sites.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144283108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium Antagonism Potential and Metabolomics Analysis of Endophytic bacteria isolated from Crotalaria retusa L., a traditional medicinal plant in Côte d'Ivoire. Côte科特迪瓦传统药用植物Crotalaria retusa L.内生细菌拮抗镰刀菌潜能及代谢组学分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-06-10 DOI: 10.1093/femsle/fnaf056

Evrad Sausthène Seka Ahoty, Romain Kouakou Fossou, Florent Magot, Anicet Théodore Ediman Ebou, Claude Ghislaine Zaka Kouadjo-Zézé, Baptiste Marchesseau, Rosella Spina, Jérémy Grosjean, Dominique Laurain-Mattar, Sophie Slezack, Adolphe Zézé

{"title":"Fusarium Antagonism Potential and Metabolomics Analysis of Endophytic bacteria isolated from Crotalaria retusa L., a traditional medicinal plant in Côte d'Ivoire.","authors":"Evrad Sausthène Seka Ahoty, Romain Kouakou Fossou, Florent Magot, Anicet Théodore Ediman Ebou, Claude Ghislaine Zaka Kouadjo-Zézé, Baptiste Marchesseau, Rosella Spina, Jérémy Grosjean, Dominique Laurain-Mattar, Sophie Slezack, Adolphe Zézé","doi":"10.1093/femsle/fnaf056","DOIUrl":"https://doi.org/10.1093/femsle/fnaf056","url":null,"abstract":"Sixty-four endophytic bacteria were isolated from roots, stems, and leaves of Crotalaria retusa L., a medicinal plant well-known for its antimicrobial properties in Côte d'Ivoire. Taxonomic characterisation revealed that these bacteria were mainly dominated by the genera Pseudomonas, Rhizobium, Bacillus and Inquilinus. The antagonistic activities of the endophytic bacteria against 2 phytopathogenic fungi affiliated with the genus Fusarium were tested using in vitro co-culture. Isolates belonging to the genus Inquilinus showed the highest inhibitory activities against Fusarium oxysporum f. sp. cubense, ranging from 40% to 57%, while the highest inhibitory activities against Fusarium graminearum were obtained with Bacillus isolates (∼66%). Finally, a metabolomic study of the leaves, stems, and seeds of the plant and of the endophytes presenting antifungal activity was carried out using LC-MS/MS analysis of the methanolic extracts of all active endophytic isolates. The identified metabolites of interest from the endophytes were mainly peptides, lipids, and steroids. Two pyrrolizidine alkaloids, monocrotaline and senecionine, were detected in the plant organs but not in the endophytic bacterial extracts. These results highlighted the potential of Crotalaria retusa L. plant and its endophytic microbiome as a source of bioactive molecules of interest and biocontrol agents against phytopathogenic Fusarium spp.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces. 用于取样方法的消毒剂中和缓冲液对表面粘附单核增生李斯特菌细胞活力的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-05-19 DOI: 10.1093/femsle/fnaf048

Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet

{"title":"Impact of disinfectant-neutralizing buffers used for sampling methods on the viability of adherent Listeria monocytogenes cells on surfaces.","authors":"Thomas Brauge, Eglantine Chalivat, Guylaine Leleu, Anthony Colas, Graziella Midelet","doi":"10.1093/femsle/fnaf048","DOIUrl":"https://doi.org/10.1093/femsle/fnaf048","url":null,"abstract":"This study assessed the impact of six disinfectant-neutralizing buffers on Listeria monocytogenes adhering to stainless steel surfaces treated with quaternary ammonium or hydrogen peroxide-based disinfectants. The goal was to evaluate potential stressors induced by these buffers during sampling, minimizing false negatives in food industry surface monitoring. Neutralizing buffers are essential in preserving bacterial viability during sample transport by counteracting residual disinfectants. L. monocytogenes populations were quantified immediately after sampling and after 24-hour incubation at 8°C, simulating transport conditions. While neutralizing buffers had minimal impact on untreated adherent cells, they significantly reduced mortality in disinfectant-treated cells. However, most buffers failed to preserve viable culturable (VC) populations after disinfection, instead promoting viable but non-culturable (VBNC) states. Notably, prolonged incubation in the Sponge neutralizer facilitated VC population recovery, likely through VBNC resuscitation or VC growth. In contrast, other buffers inhibited recovery, suggesting detrimental effects on stressed cells due to their chemical composition. These findings underscore the importance of selecting appropriate neutralizing buffers in L. monocytogenes detection, influencing food safety surveillance and risk assessment protocols.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability. MEEhubs2024：以中心为基础的微生物生态学和进化促进可持续性会议。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf022

Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius

{"title":"MEEhubs2024: A hub-based conference on microbial ecology and evolution fostering sustainability.","authors":"Ariane Wenger, Erik Bakkeren, Elisa Granato, Robin Tecon, Sara Mitri, Wolfram Möbius","doi":"10.1093/femsle/fnaf022","DOIUrl":"10.1093/femsle/fnaf022","url":null,"abstract":"Scientific conferences are essential to academic exchange. However, related air travel contributes to greenhouse gas emissions, while expensive registration and travel costs limit the participation of early career researchers and those from low-income countries. Virtual conferences offer promising solutions for reducing emissions and enhancing accessibility and inclusivity but often limit networking and personal interaction. Hybrid multi-hub conferences, which combine virtually connected in-person venues with individual virtual participation, combine the benefits of both conference formats. Thus, we present and discuss MEEhubs2024, a multi-hub conference on microbial ecology and evolution held in January 2024. During this 3-day conference, attendees participated virtually or at one of six hubs in Europe and North America. We analyzed the participants' and organizers' feedback to create a template and provide insights into the scientific community's adoption of this new conference format, which was positively evaluated by most participants. Because technical, logistical, and structural challenges remain, including limited opportunities to interact and network across hubs and participation modes, we provide recommendations for improvement, such as hiring technical hosts and offering virtual-only social activities. Finally, we used the participants' feedback to reflect on conference expectations, highlighting research gaps and the need for organizers to define and communicate goals when organizing conferences.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency. 在浸泡过的食物垃圾中，曝气可以促进变形杆菌而不是厚壁菌，从而产生优越的厌氧消化效率。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf001

Linjie Tang, Mike Manefield

{"title":"Aeration promotes Proteobacteria over Firmicutes in macerated food waste, resulting in superior anaerobic digestion efficiency.","authors":"Linjie Tang, Mike Manefield","doi":"10.1093/femsle/fnaf001","DOIUrl":"10.1093/femsle/fnaf001","url":null,"abstract":"Aeration is a common pretreatment method to enhance biogas production via anaerobic digestion of waste organic feedstocks such as unused food. While impacts on downstream anaerobic digestion have been intensively investigated, the consequence of aeration on the microbial community in food waste has not been characterized. Food waste has a low pH resulting from the dominance of lactic acid bacteria within the Firmicutes phylum. This excludes other phylotypes with a higher potential to hydrolyse complex biopolymers in food waste. In this study, we reveal that aeration of macerated food waste results in a dramatic shift away from Firmicutes towards dominance of Proteobacteria that are better known for extracellular enzyme production. Given that hydrolysis is the rate limiting step in anaerobic digestion, this explains why aeration improves the efficiency of biogas production from food waste. The discovery that Proteobacteria dominate microbial communities in aerated food waste opens up opportunities to manipulate extracellular enzyme production through gene expression mechanisms common among Proteobacteria such as quorum sensing.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142964311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA. 细小脲原体SMC蛋白与DNA相互作用的特性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf010

Natalia A Rumyantseva, Vladimir M Shutov, Dina G Belenkaia, Aleksandr A Alekseev, Natalia E Morozova, Alexey D Vedyaykin

引用次数: 0

In vitro evolution of ciprofloxacin resistance in Neisseria commensals and derived mutation population dynamics in natural Neisseria populations. 共生奈瑟菌环丙沙星耐药性体外进化及自然奈瑟菌群体衍生突变种群动态。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae107

Leah R Robinson, Caroline J McDevitt, Molly R Regan, Sophie L Quail, Crista B Wadsworth

{"title":"In vitro evolution of ciprofloxacin resistance in Neisseria commensals and derived mutation population dynamics in natural Neisseria populations.","authors":"Leah R Robinson, Caroline J McDevitt, Molly R Regan, Sophie L Quail, Crista B Wadsworth","doi":"10.1093/femsle/fnae107","DOIUrl":"10.1093/femsle/fnae107","url":null,"abstract":"Commensal Neisseria are members of a healthy human oropharyngeal microbiome; however, they also serve as a reservoir of antimicrobial resistance for their pathogenic relatives. Despite their known importance as sources of novel genetic variation for pathogens, we still do not understand the full suite of resistance mutations commensal species can harbor. Here, we use in vitro selection to assess the mutations that emerge in response to ciprofloxacin selection in commensal Neisseria by passaging four replicates of four different species in the presence of a selective antibiotic gradient for 20 days; then categorized derived mutations with whole genome sequencing. Ten out of sixteen selected cells lines across the four species evolved ciprofloxacin resistance (≥1 ug/ml); with resistance-contributing mutations primarily emerging in DNA gyrase subunit A and B (gyrA and gyrB), topoisomerase IV subunits C and E (parC and parE), and the multiple transferable efflux pump repressor (mtrR). Of note, these derived mutations appeared in the same loci responsible for ciprofloxacin-reduced susceptibility in the pathogenic Neisseria, suggesting conserved mechanisms of resistance across the genus. Additionally, we tested for zoliflodacin cross-resistance in evolved strain lines and found 6 lineages with elevated zoliflodacin minimum inhibitory concentrations. Finally, to interrogate the likelihood of experimentally derived mutations emerging and contributing to resistance in natural Neisseria, we used a population-based approach and identified GyrA 91I as a substitution circulating within commensal Neisseria populations and ParC 85C in a single gonococcal isolate. A small cluster of gonococcal isolates shared commensal alleles at parE, suggesting recent cross-species recombination events.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11774118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly synthesis of silver nanoparticles using lignin peroxidase produced from Caldibacillus thermoamylovorans and cultivated on waste walnut shell. 利用热淀粉样芽孢杆菌产生的木质素过氧化物酶在废核桃壳上的生态合成纳米银。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf036

Sefa Nur Akkaya, Ammar Almansour, Mehmet Akif Omeroglu, Hayrunnisa Nadaroglu, Ahmet Adiguzel

{"title":"Eco-friendly synthesis of silver nanoparticles using lignin peroxidase produced from Caldibacillus thermoamylovorans and cultivated on waste walnut shell.","authors":"Sefa Nur Akkaya, Ammar Almansour, Mehmet Akif Omeroglu, Hayrunnisa Nadaroglu, Ahmet Adiguzel","doi":"10.1093/femsle/fnaf036","DOIUrl":"10.1093/femsle/fnaf036","url":null,"abstract":"The preparation of silver nanoparticles (AgNPs) via an environmentally friendly green synthesis method represents an ecologically promising alternative. This research aims to develop sustainable and eco-friendly AgNPs using the lignin peroxidase (LiP) enzyme from Caldibacillus thermoamylovorans, cultivated on waste walnut shells, which are rich in lignin, to meet the growing demand for AgNPs. Among thermophilic bacteria that were isolated, the C. thermoamylovorans SA1 strain showed the highest LiP activity. The production of LiP was optimized by adding waste walnut shells and manipulating the environmental parameters. The optimal conditions were determined at 50 g/l shell amount, 96 h, pH 8, 140 rpm, and 60°C. In parallel with the increase in enzyme activity, bacterial growth also increased. As a result of the optimization, the highest enzyme activity value was 435.0 U/ml and bacterial growth was determined to be OD600: 2.09. The extracellular medium obtained from the bacteria grown in walnut shell medium was then added to an AgNO3 solution. Efficient production of AgNPs was achieved by stirring the mixture at 50°C-60°C for 4 h under optimum conditions. The synthesized AgNPs were characterized using a range of analytical techniques, including UV‒Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The biological efficacy of the synthesized AgNPs was evaluated by assessing their antibacterial activity against pathogenic bacteria, such as Escherichia coli O157:H7, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pyogenes, and Bacillus cereus. The highest activity was observed against B. cereus (15 mm). The broad-spectrum antibacterial properties exhibited by the AgNPs synthesized in this study offer a promising and sustainable solution for diverse applications in various sectors, including the environmental, agricultural, medical, and pharmaceutical fields.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria. 植物酚类化合物对氨基酸发酵菌产氨的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf018

Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe

{"title":"Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria.","authors":"Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe","doi":"10.1093/femsle/fnaf018","DOIUrl":"10.1093/femsle/fnaf018","url":null,"abstract":"Bacteria that ferment amino acids to ammonia can be categorized as generalists or specialist hyper-ammonia-producing bacteria. In the rumens of ruminant animals, most of the ammonia produced is eventually excreted as urea in urine. This process can be controlled with off-label use of antibiotics, but the practice can lead to antibiotic resistance; therefore, discovery of antibiotic alternatives is pertinent. Plant-derived phenolic compounds have demonstrated antimicrobial efficacy for such purposes. This study investigated the antimicrobial and metabolic suppressive potential of six phenolic compounds on five amino acid fermenting bacteria: Clostridium sporogenes MD1, C. aminophilum F, Acetoanaerobium sticklandii SR, Peptostreptococcus sp. BG1, and Prevotella bryantii B14. Inhibitory action of the compounds was determined using a 10% v/v serial dilution method in basal media. Carvacrol (1 mM), thymol (1 mM), and eugenol (10 mM) demonstrated the greatest antimicrobial potential, where carvacrol and eugenol inhibited growth of all five species and thymol four species except BG1. The cinnamic acids (trans and hydro) demonstrated variable activity against all organisms. Suppression of metabolic activity was determined via colorimetric assay quantifying ammonia in washed stationary phase culture supernatant after 24 h of metabolism on fresh substrate. Carvacrol and eugenol yielded the greatest reduction of ammonia by all organisms except B14, which produced no ammonia under the growth conditions. Thymol greatly reduced ammonia production of four organisms except F. These data demonstrate that eugenol, carvacrol, and thymol may be worthy antimicrobial candidates for the control of ammonia-producing organisms.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0