Fems Microbiology Letters最新文献_第2页

Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA. 细小脲原体SMC蛋白与DNA相互作用的特性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf010

Natalia A Rumyantseva, Vladimir M Shutov, Dina G Belenkaia, Aleksandr A Alekseev, Natalia E Morozova, Alexey D Vedyaykin

引用次数: 0

Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples. 菲达干酪植物乳杆菌细菌素基因及抑菌活性测定。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf002

Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel

{"title":"Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples.","authors":"Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel","doi":"10.1093/femsle/fnaf002","DOIUrl":"10.1093/femsle/fnaf002","url":null,"abstract":"In this study designed to isolate lactic acid bacteria (LAB) with bacteriocin production potential, white cheese samples were collected from different provinces of Turkey and isolation was carried out. A series of experiments were carried out for the main purpose and the actual bacteriocin producers were identified by detecting the genes encoding this bacteriocin. The experiments carried out in this direction were initially carried out with 20 isolates and as a result of various experiments, the number of isolates was reduced to 8 and the study was continued with 8 isolates. In order to determine that the eight isolates identified as a result of a phenotypic and biochemical characterization study were true bacteriocin-producing strains, their antibacterial activity was investigated and then the presence of bacteriocin genes was examined by specific polymerase chain reaction (PCR) using gene-specific primers. As a result, MS16 coded Lactiplantibacillus plantarum OR922652 was found to have strong antibacterial activity against Escherichia coli, Klebsiella pneumonia, Yersinia enterocolitica, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus; the isolate was susceptible to clinically important antibiotics (ciprofloxacin, gentamicin, penicillin G, ampicillin, chloramphenicol, and vancomycin) and resistant to erythromycin, had no hemolytic activity and possessed plnA and plnD genes encoding bacteriocin production. In conclusion, the MS16 coded L. plantarum isolate has emerged as a promising strain that can be used especially in the health field and in the food industry related to LAB.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid and integron-associated antibiotic resistance in Escherichia coli isolated from domestic wastewater treatment plants. 生活污水处理厂分离的大肠杆菌质粒和整合子相关抗生素耐药性研究

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf041

Bradd Mendoza-Guido, Luis Rivera-Montero, Kenia Barrantes, Luz Chacon

{"title":"Plasmid and integron-associated antibiotic resistance in Escherichia coli isolated from domestic wastewater treatment plants.","authors":"Bradd Mendoza-Guido, Luis Rivera-Montero, Kenia Barrantes, Luz Chacon","doi":"10.1093/femsle/fnaf041","DOIUrl":"https://doi.org/10.1093/femsle/fnaf041","url":null,"abstract":"The rapid dissemination of antibiotic resistance genes (ARGs) represents a significant global threat, with wastewater treatment plants (WWTPs) playing an important role as reservoirs and propagation hubs. In this study, we performed whole-genome sequencing and bioinformatic analyses on eight multidrug-resistant Escherichia coli isolates previously obtained from domestic WWTPs in Costa Rica. We identified 61 ARGs (23 unique), with 40 located on plasmids, and 21 on chromosomal sequences, seven of which were within integrons. Several ARGs were associated with resistance to clinically and veterinary important antibiotics, including sulfamethoxazole/trimethoprim, beta-lactams, and tetracyclines. One hundred twenty-one virulence-associated genes (29 unique) were detected, with 16 located on plasmids. Notably, the presence of virulence factors such as ompT and hlyF genes alongside ARGs on plasmids underscores the transmissible pathogenic potential of WWTP-associated E. coli strains. These findings highlight the role of small domestic WWTPs in disseminating pathogenic and multidrug-resistant bacteria and their mobile genetic elements, emphasizing the need for further research to understand how these discharges impact aquatic environments.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria. 修正：植物酚类化合物对氨基酸发酵菌产氨的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf046

引用次数: 0

Improving the thermal tolerance of biocontrol spores, Akanthomyces lecanii, by encapsulation. 用包封法提高生防孢子的耐热性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf062

Paul W Baker, Ephraim Nuwamanya, Adam Charlton

{"title":"Improving the thermal tolerance of biocontrol spores, Akanthomyces lecanii, by encapsulation.","authors":"Paul W Baker, Ephraim Nuwamanya, Adam Charlton","doi":"10.1093/femsle/fnaf062","DOIUrl":"10.1093/femsle/fnaf062","url":null,"abstract":"Akanthomyces lecanii is an entomopathogenic fungus, and spores of this fungus could be incorporated into films generated using cast film extrusion for biocontrol applications. However, the extrusion process involves high temperature processing (150°C) although this only lasts for a few minutes. The elevated temperature destroys spores, thereby eliminating functionality, unless the spores are protected from this heat. Initial experiments revealed that the heat tolerance of free A. lecanii spores to be 60°C. The spores were therefore encapsulated into beads prepared using a combination of Gelrite, cellulose, and Cel-fine at different concentrations. The beads were freeze-dried and then immersed in hot glycerol for 2 min at a selected temperature within the range of 50°C-100°C. The results indicated that some combinations of encapsulating agents resulted in the spores retaining viability (plate counting) after heat treatment at 100°C. Beads stored at room temperature for 1 week showed a reduction in the upper temperature tolerance. This study revealed that the temperature tolerance of A. lecanii spores could be improved by 40°C by encapsulation in freeze-dried beads containing 2% Gelrite (purified gellum gum), 0.4% cellulose, and 0.4% Cel-fine.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144301473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Standardized analysis of nuclease activities in Mycoplasma species colonizing swine, poultry, and small ruminants. 猪、家禽和小反刍动物支原体定殖核酸酶活性的标准化分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf057

Manon Vastel, Patrice Gaurivaud, Florence Tardy, Anne V Gautier-Bouchardon

{"title":"Standardized analysis of nuclease activities in Mycoplasma species colonizing swine, poultry, and small ruminants.","authors":"Manon Vastel, Patrice Gaurivaud, Florence Tardy, Anne V Gautier-Bouchardon","doi":"10.1093/femsle/fnaf057","DOIUrl":"10.1093/femsle/fnaf057","url":null,"abstract":"Secreted nucleases, either cell-attached or released in the environment, play varied roles in bacteria-host interactions during an infection. They help to provide nucleotides essential for bacterial growth by degrading host nucleic acids, are involved in the degradation of extracellular traps and hence in immune evasion, and can have direct cytotoxic activity in host cells. Nuclease expression in bacteria of the Mycoplasma genus has been poorly studied so far. In this study, a standardized set of methods was used to detect the in vitro nuclease activities of major animal mycoplasmas. Nucleases were detected in swine (Mycoplasma (M.) hyopneumoniae, M. hyorhinis, and M. flocculare) as well as avian (M. gallisepticum, M. iowae, and M. synoviae) species, but not in the small ruminant subspecies M. mycoides subsp. capri and M. capricolum subsp. capricolum. In swine species, nuclease activity was detected in both the cell pellet and the supernatant, whereas in poultry species, the results were more variable. We showed that detection of nuclease activity-in terms of presence/absence in our experimental conditions-was strain dependent in M. iowae and M. synoviae. The DNA from macrophage extracellular traps was further demonstrated to be a substrate for mycoplasma-expressed nucleases, suggesting that several Mycoplasma species and/or strains infecting animals could feed on resulting nucleotides and hence escape the traps, two features contributing to persistence of the infection.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144257696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo-a pilot study. 微双孢菌素（NAI-107）在斑马鱼（Danio rerio）胚胎中的安全性初步评价。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf047

Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil

{"title":"Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo-a pilot study.","authors":"Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil","doi":"10.1093/femsle/fnaf047","DOIUrl":"10.1093/femsle/fnaf047","url":null,"abstract":"NAI-107 has demonstrated in vitro and in vivo efficacy against multidrug-resistance (MDR) pathogens. Studies using the Galleria mellonella model found NAI-107 effective against Neisseria gonorrhoeae and vanB-resistant Enterococcus faecium, with no toxicity at concentrations up to 64 µg/ml. This pilot study evaluated NAI-107's safety using transgenic zebrafish embryos (kdrl:eGFP) that were exposed to NAI-107 concentrations of 0, 10, 20, and 30 µM from 5 hours post-fertilization (hpf) to 4 days post-fertilization (dpf). No mortality or significant morphological abnormalities were observed across all treatment groups. At 2 dpf, larvae treated with 20 and 30 µM NAI-107 exhibited a significant increase in heart rate (P < 0.05), although other cardiac parameters remain unaffected. At 4 dpf, larvae displayed no significant growth differences but had increased locomotor activity at 20 and 30 µM NAI-107 groups, indicated by the higher total distance moved and mean velocity (P < 0.05). NAI-107, at concentrations up to 30 µM, did not induce significant toxic effects in zebrafish embryos. The absence of adverse impacts on survival or gross morphology suggests a favorable safety margin. However, the observed increases in heart rate and locomotor activity suggest possible subclinical effects at higher concentrations that warrant further investigation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa maintains an inducible array of novel and diverse prophages over lengthy persistence in cystic fibrosis lungs. 铜绿假单胞菌在囊性纤维化肺中维持一种可诱导的新型和多样化的噬菌体阵列。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf017

Ifigeneia Kyrkou, Jennifer Bartell, Ana Lechuga, Cédric Lood, Rasmus L Marvig, Rob Lavigne, Søren Molin, Helle Krogh Johansen

{"title":"Pseudomonas aeruginosa maintains an inducible array of novel and diverse prophages over lengthy persistence in cystic fibrosis lungs.","authors":"Ifigeneia Kyrkou, Jennifer Bartell, Ana Lechuga, Cédric Lood, Rasmus L Marvig, Rob Lavigne, Søren Molin, Helle Krogh Johansen","doi":"10.1093/femsle/fnaf017","DOIUrl":"10.1093/femsle/fnaf017","url":null,"abstract":"Pseudomonas aeruginosa has increasing clinical relevance and commonly occupies the cystic fibrosis (CF) airways. Its ability to colonize and persist in diverse niches is attributed to its large accessory genome, where prophages represent a common feature and may contribute to its fitness and persistence. We focused on the CF airways niche and used 197 longitudinal isolates from 12 patients persistently infected by P. aeruginosa. We computationally predicted intact prophages for each longitudinal group and scored their long-term persistence. We then confirmed prophage inducibility and mapped their location in the host chromosome with lysate sequencing. Using comparative genomics, we evaluated prophage genomic diversity, long-term persistence, and level of genomic maintenance. Our findings support previous findings that most P. aeruginosa genomes harbour prophages some of which can self-induce, and that a common CF-treating antibiotic, ciprofloxacin, can induce prophages. Induced prophage genomes displayed high diversity and even genomic novelty. Finally, all induced prophages persisted long-term with their genomes avoiding gene loss and degradation over 4 years of host replication in the stressful CF airways niche. This and our detection of phage genes, which contribute to host competitiveness and adaptation, lends support to our hypothesis that the vast majority of prophages detected as intact and inducible in this study facilitated their host fitness and persistence.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azospirillum brasilense and cytidine enhance lateral roots of peas. 巴西氮螺旋菌和胞苷增强豌豆侧根。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf025

Fatema A Nisha, Shelley M Horne, Birgit M Prüß

{"title":"Azospirillum brasilense and cytidine enhance lateral roots of peas.","authors":"Fatema A Nisha, Shelley M Horne, Birgit M Prüß","doi":"10.1093/femsle/fnaf025","DOIUrl":"10.1093/femsle/fnaf025","url":null,"abstract":"Azospirillum brasilense is a plant growth beneficial rhizobacterium (PGBR) that is used as an inoculant to enhance root architecture in grassland and crop plants. The intent of our study was to develop A. brasilense into a probiotic inoculant for peas and supplement with a seedling exudate compound, to be used together or separately. As an initial characterization of the association of A. brasilense with pea roots, we performed several pea growth experiments. Azospirillum brasilense Sp7T increased the lengths of the five longest lateral roots from each plant by 63.6% and the top 10 lateral roots across 14 plants by 30%, an effect that was abolished in an rpoN mutant and a ΔcheA1/cheA4 mutant. Azospirillum brasilense Cd increased the number of lateral roots by 76%. We detected colonization by this PGBR within the epiphytic root microbiome. To identify a pea seedling exudate compound capable of enhancing lateral pea roots, we tested 15 such compounds. Cytidine was the only one that increased the number of lateral roots, by approximately two-fold, an effect that did not require A. brasilense. We conclude that both A. brasilense and cytidine might be suitable as supplements to enhance lateral roots of pea plants.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of crystal violet staining, microscopy with image analysis, and quantitative PCR to examine biofilm dynamics. 比较结晶紫染色，显微镜与图像分析，定量PCR检查生物膜动力学。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae115

So-Yeon Jeong, Ji Won Lee, Eun Ji Kim, Chi Won Lee, Tae Gwan Kim

{"title":"Comparison of crystal violet staining, microscopy with image analysis, and quantitative PCR to examine biofilm dynamics.","authors":"So-Yeon Jeong, Ji Won Lee, Eun Ji Kim, Chi Won Lee, Tae Gwan Kim","doi":"10.1093/femsle/fnae115","DOIUrl":"10.1093/femsle/fnae115","url":null,"abstract":"Crystal-violet staining, microscopy with image analysis, and quantitative PCR (qPCR) were compared to examine biofilm dynamics. Biofilms of 30 polycultures comprising 15 bacterial species were monitored for 14 days. Collectively, qPCR (representing population) revealed a different growth pattern compared to staining (biomass) and microscopy (colonization): biomass and colonization gradually increased over time, whereas population increased rapidly for the first seven days and leveled off. Temporal forms were categorized into two growth patterns: continuous increase (CI) and non-continuous increase. Staining and microscopy showed similar odds of detecting the CI pattern (27 and 23 polycultures, respectively) across polycultures, greater than that of qPCR (14 polycultures) (P < 0.05). All three methods revealed the identical patterns for 13 polycultures. Staining with microscopy, staining with qPCR, and microscopy with qPCR found the same patterns in 22, 15, and 19 polycultures, respectively. Additionally, staining was quantitatively agreed with microscopy (P < 0.05; R2 > 0.50), whereas neither staining nor microscopy strongly agreed with qPCR (P < 0.05; R2 ≤ 0.22). Collectively, staining was more compatible with microscopy than qPCR in characterizing biofilm dynamics and quantifying biofilms owing to the difference between population growth and biofilm expansion. The concurrent use of qPCR with biomass estimations allows for accurate and comprehensive biofilm quantification.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0