Fems Microbiology Letters最新文献_第2页

Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria. 植物酚类化合物对氨基酸发酵菌产氨的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf018

Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe

{"title":"Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria.","authors":"Jourdan E Lakes, Leah I Ramos, Maedean L Cardenas, Natasha L Mast, Michael D Flythe","doi":"10.1093/femsle/fnaf018","DOIUrl":"10.1093/femsle/fnaf018","url":null,"abstract":"Bacteria that ferment amino acids to ammonia can be categorized as generalists or specialist hyper-ammonia-producing bacteria. In the rumens of ruminant animals, most of the ammonia produced is eventually excreted as urea in urine. This process can be controlled with off-label use of antibiotics, but the practice can lead to antibiotic resistance; therefore, discovery of antibiotic alternatives is pertinent. Plant-derived phenolic compounds have demonstrated antimicrobial efficacy for such purposes. This study investigated the antimicrobial and metabolic suppressive potential of six phenolic compounds on five amino acid fermenting bacteria: Clostridium sporogenes MD1, C. aminophilum F, Acetoanaerobium sticklandii SR, Peptostreptococcus sp. BG1, and Prevotella bryantii B14. Inhibitory action of the compounds was determined using a 10% v/v serial dilution method in basal media. Carvacrol (1 mM), thymol (1 mM), and eugenol (10 mM) demonstrated the greatest antimicrobial potential, where carvacrol and eugenol inhibited growth of all five species and thymol four species except BG1. The cinnamic acids (trans and hydro) demonstrated variable activity against all organisms. Suppression of metabolic activity was determined via colorimetric assay quantifying ammonia in washed stationary phase culture supernatant after 24 h of metabolism on fresh substrate. Carvacrol and eugenol yielded the greatest reduction of ammonia by all organisms except B14, which produced no ammonia under the growth conditions. Thymol greatly reduced ammonia production of four organisms except F. These data demonstrate that eugenol, carvacrol, and thymol may be worthy antimicrobial candidates for the control of ammonia-producing organisms.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12072259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara. 突尼斯撒哈拉沙漠南部两个地热绿洲嗜盐古菌群落的元分类分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae106

Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari

{"title":"Metataxonomic analysis of halophilic archaea community in two geothermal oases in the southern Tunisian Sahara.","authors":"Afef Najjari, Khaled Elmnasri, Hanene Cherif, Stephen Burleigh, Amel Guesmi, Mouna Mahjoubi, Javier A Linares-Pastén, Ameur Cherif, Hadda-Imene Ouzari","doi":"10.1093/femsle/fnae106","DOIUrl":"10.1093/femsle/fnae106","url":null,"abstract":"This study assesses halophilic archaea's phylogenetic diversity in southern Tunisia's geothermal water. In the arid southern regions, limited surface freshwater resources make geothermal waters a vital source for oases and greenhouse irrigation. Three samples, including water, sediment, and halite soil crust, were collected downstream of two geothermal springs of the Ksar Ghilane (KGH) and Zaouet Al Aness (ZAN) oases, Tunisia. The samples were subjected to 16S rRNA gene sequencing using the Illumina Miseq sequencing approach. Several haloarchaea were identified in the geothermal springs. The average taxonomic composition revealed that 20 out of 33 genera were shared between the two geothermal sources, with uneven distribution, where the Halogranum genus was the most represented genus with an abundance of 18.9% and 11.58% for ZAW and KGH, respectively. Several unique site-specific genera were observed: Halonotius, Halopelagius, Natronorubrum, and Haloarcula in ZAN, and Haloprofundus, Halomarina, Halovivax, Haloplanus, Natrinema, Halobium, Natronoarchaeum, and Haloterrigena in the KGH pool. Most genus members are typically found in low-salinity ecosystems. These findings suggest that haloarchaea can disperse downstream from geothermal sources and may survive temperature and chemical fluctuations in the runoff.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa maintains an inducible array of novel and diverse prophages over lengthy persistence in cystic fibrosis lungs. 铜绿假单胞菌在囊性纤维化肺中维持一种可诱导的新型和多样化的噬菌体阵列。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf017

Ifigeneia Kyrkou, Jennifer Bartell, Ana Lechuga, Cédric Lood, Rasmus L Marvig, Rob Lavigne, Søren Molin, Helle Krogh Johansen

{"title":"Pseudomonas aeruginosa maintains an inducible array of novel and diverse prophages over lengthy persistence in cystic fibrosis lungs.","authors":"Ifigeneia Kyrkou, Jennifer Bartell, Ana Lechuga, Cédric Lood, Rasmus L Marvig, Rob Lavigne, Søren Molin, Helle Krogh Johansen","doi":"10.1093/femsle/fnaf017","DOIUrl":"10.1093/femsle/fnaf017","url":null,"abstract":"Pseudomonas aeruginosa has increasing clinical relevance and commonly occupies the cystic fibrosis (CF) airways. Its ability to colonize and persist in diverse niches is attributed to its large accessory genome, where prophages represent a common feature and may contribute to its fitness and persistence. We focused on the CF airways niche and used 197 longitudinal isolates from 12 patients persistently infected by P. aeruginosa. We computationally predicted intact prophages for each longitudinal group and scored their long-term persistence. We then confirmed prophage inducibility and mapped their location in the host chromosome with lysate sequencing. Using comparative genomics, we evaluated prophage genomic diversity, long-term persistence, and level of genomic maintenance. Our findings support previous findings that most P. aeruginosa genomes harbour prophages some of which can self-induce, and that a common CF-treating antibiotic, ciprofloxacin, can induce prophages. Induced prophage genomes displayed high diversity and even genomic novelty. Finally, all induced prophages persisted long-term with their genomes avoiding gene loss and degradation over 4 years of host replication in the stressful CF airways niche. This and our detection of phage genes, which contribute to host competitiveness and adaptation, lends support to our hypothesis that the vast majority of prophages detected as intact and inducible in this study facilitated their host fitness and persistence.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846083/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples. 菲达干酪植物乳杆菌细菌素基因及抑菌活性测定。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf002

Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel

{"title":"Determination of bacteriocin genes and antimicrobial activity of Lactiplantibacillus plantarum isolated from feta cheese samples.","authors":"Sumeyye Akbulut, Elanur Dasdemir, Hakan Ozkan, Ahmet Adiguzel","doi":"10.1093/femsle/fnaf002","DOIUrl":"10.1093/femsle/fnaf002","url":null,"abstract":"In this study designed to isolate lactic acid bacteria (LAB) with bacteriocin production potential, white cheese samples were collected from different provinces of Turkey and isolation was carried out. A series of experiments were carried out for the main purpose and the actual bacteriocin producers were identified by detecting the genes encoding this bacteriocin. The experiments carried out in this direction were initially carried out with 20 isolates and as a result of various experiments, the number of isolates was reduced to 8 and the study was continued with 8 isolates. In order to determine that the eight isolates identified as a result of a phenotypic and biochemical characterization study were true bacteriocin-producing strains, their antibacterial activity was investigated and then the presence of bacteriocin genes was examined by specific polymerase chain reaction (PCR) using gene-specific primers. As a result, MS16 coded Lactiplantibacillus plantarum OR922652 was found to have strong antibacterial activity against Escherichia coli, Klebsiella pneumonia, Yersinia enterocolitica, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus; the isolate was susceptible to clinically important antibiotics (ciprofloxacin, gentamicin, penicillin G, ampicillin, chloramphenicol, and vancomycin) and resistant to erythromycin, had no hemolytic activity and possessed plnA and plnD genes encoding bacteriocin production. In conclusion, the MS16 coded L. plantarum isolate has emerged as a promising strain that can be used especially in the health field and in the food industry related to LAB.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmid and integron-associated antibiotic resistance in Escherichia coli isolated from domestic wastewater treatment plants. 生活污水处理厂分离的大肠杆菌质粒和整合子相关抗生素耐药性研究

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf041

Bradd Mendoza-Guido, Luis Rivera-Montero, Kenia Barrantes, Luz Chacon

{"title":"Plasmid and integron-associated antibiotic resistance in Escherichia coli isolated from domestic wastewater treatment plants.","authors":"Bradd Mendoza-Guido, Luis Rivera-Montero, Kenia Barrantes, Luz Chacon","doi":"10.1093/femsle/fnaf041","DOIUrl":"https://doi.org/10.1093/femsle/fnaf041","url":null,"abstract":"The rapid dissemination of antibiotic resistance genes (ARGs) represents a significant global threat, with wastewater treatment plants (WWTPs) playing an important role as reservoirs and propagation hubs. In this study, we performed whole-genome sequencing and bioinformatic analyses on eight multidrug-resistant Escherichia coli isolates previously obtained from domestic WWTPs in Costa Rica. We identified 61 ARGs (23 unique), with 40 located on plasmids, and 21 on chromosomal sequences, seven of which were within integrons. Several ARGs were associated with resistance to clinically and veterinary important antibiotics, including sulfamethoxazole/trimethoprim, beta-lactams, and tetracyclines. One hundred twenty-one virulence-associated genes (29 unique) were detected, with 16 located on plasmids. Notably, the presence of virulence factors such as ompT and hlyF genes alongside ARGs on plasmids underscores the transmissible pathogenic potential of WWTP-associated E. coli strains. These findings highlight the role of small domestic WWTPs in disseminating pathogenic and multidrug-resistant bacteria and their mobile genetic elements, emphasizing the need for further research to understand how these discharges impact aquatic environments.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Effects of phyto-phenolic compounds on ammonia production by select amino acid fermenting bacteria. 修正：植物酚类化合物对氨基酸发酵菌产氨的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf046

引用次数: 0

Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo-a pilot study. 微双孢菌素（NAI-107）在斑马鱼（Danio rerio）胚胎中的安全性初步评价。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf047

Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil

{"title":"Safety evaluation of microbisporicin (NAI-107) using zebrafish (Danio rerio) embryo-a pilot study.","authors":"Zina Gestels, Saïd Abdellati, Andy Willaert, Chris Kenyon, Sheeba Santhini Manoharan-Basil","doi":"10.1093/femsle/fnaf047","DOIUrl":"10.1093/femsle/fnaf047","url":null,"abstract":"NAI-107 has demonstrated in vitro and in vivo efficacy against multidrug-resistance (MDR) pathogens. Studies using the Galleria mellonella model found NAI-107 effective against Neisseria gonorrhoeae and vanB-resistant Enterococcus faecium, with no toxicity at concentrations up to 64 µg/ml. This pilot study evaluated NAI-107's safety using transgenic zebrafish embryos (kdrl:eGFP) that were exposed to NAI-107 concentrations of 0, 10, 20, and 30 µM from 5 hours post-fertilization (hpf) to 4 days post-fertilization (dpf). No mortality or significant morphological abnormalities were observed across all treatment groups. At 2 dpf, larvae treated with 20 and 30 µM NAI-107 exhibited a significant increase in heart rate (P < 0.05), although other cardiac parameters remain unaffected. At 4 dpf, larvae displayed no significant growth differences but had increased locomotor activity at 20 and 30 µM NAI-107 groups, indicated by the higher total distance moved and mean velocity (P < 0.05). NAI-107, at concentrations up to 30 µM, did not induce significant toxic effects in zebrafish embryos. The absence of adverse impacts on survival or gross morphology suggests a favorable safety margin. However, the observed increases in heart rate and locomotor activity suggest possible subclinical effects at higher concentrations that warrant further investigation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reach of the Instagram profile @microbioworld in popularizing mycology and microbiology. Instagram个人资料@microbioworld在普及真菌学和微生物学方面的影响力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf019

Jefferson Brendon Almeida Dos Reis, Sofia Coradini Schirmer

{"title":"Reach of the Instagram profile @microbioworld in popularizing mycology and microbiology.","authors":"Jefferson Brendon Almeida Dos Reis, Sofia Coradini Schirmer","doi":"10.1093/femsle/fnaf019","DOIUrl":"10.1093/femsle/fnaf019","url":null,"abstract":"Online social networks have revolutionized scientific communication, making platforms like Instagram indispensable for sharing complex topics, including mycology. This study evaluated three key factors in assessing the impact of social media on scientific dissemination: follower profiles, reach, and engagement levels. We used the professional Instagram account @microbioworld as a case study. Account performance data were collected over a 90-day period (12 August-9 November 2024). Post performance was evaluated using data from selected posts published between 11 January and 11 November 2024. By the end of our sampling period, the account reached a total of 45 959 followers, with the majority aged 25-34 years (44.8%). It reached 108 631 unique accounts, with 22.4% being followers and 77.6% non-followers, generating 236 860 impressions and 15 750 interactions. Likes accounted for 83.3% of engagement. Posts featuring microorganism cultures achieved the highest engagement and reach. Sentiment analysis, using Bing and AFINN lexicons, revealed that over 89% of sentiments expressed in comments were positive. These findings demonstrate how Instagram can disseminate microbial content, foster positive perceptions of microorganisms, and emphasize their ecological importance, encouraging audience involvement with mycology and microbiology.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azospirillum brasilense and cytidine enhance lateral roots of peas. 巴西氮螺旋菌和胞苷增强豌豆侧根。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf025

Fatema A Nisha, Shelley M Horne, Birgit M Prüß

{"title":"Azospirillum brasilense and cytidine enhance lateral roots of peas.","authors":"Fatema A Nisha, Shelley M Horne, Birgit M Prüß","doi":"10.1093/femsle/fnaf025","DOIUrl":"10.1093/femsle/fnaf025","url":null,"abstract":"Azospirillum brasilense is a plant growth beneficial rhizobacterium (PGBR) that is used as an inoculant to enhance root architecture in grassland and crop plants. The intent of our study was to develop A. brasilense into a probiotic inoculant for peas and supplement with a seedling exudate compound, to be used together or separately. As an initial characterization of the association of A. brasilense with pea roots, we performed several pea growth experiments. Azospirillum brasilense Sp7T increased the lengths of the five longest lateral roots from each plant by 63.6% and the top 10 lateral roots across 14 plants by 30%, an effect that was abolished in an rpoN mutant and a ΔcheA1/cheA4 mutant. Azospirillum brasilense Cd increased the number of lateral roots by 76%. We detected colonization by this PGBR within the epiphytic root microbiome. To identify a pea seedling exudate compound capable of enhancing lateral pea roots, we tested 15 such compounds. Cytidine was the only one that increased the number of lateral roots, by approximately two-fold, an effect that did not require A. brasilense. We conclude that both A. brasilense and cytidine might be suitable as supplements to enhance lateral roots of pea plants.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

About two French cases of disseminated Cryptococcus neoformans infection associated with COVID-19. 关于2例与COVID-19相关的法国播散性新型隐球菌感染。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf012

Wendy Pulby, Jérémy Lafolie, Chloé Belot, Loïc Dopeux, Sébastien Loiseau, Maxime Moniot, Philippe Poirier, Céline Nourrisson

引用次数: 0