Properties of the Ureaplasma parvum structural maintenance of chromosomes protein related to its interaction with DNA.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2025-01-10 DOI:10.1093/femsle/fnaf010

Natalia A Rumyantseva, Vladimir M Shutov, Dina G Belenkaia, Aleksandr A Alekseev, Natalia E Morozova, Alexey D Vedyaykin

引用次数: 0

Abstract

SMC (Structural Maintenance of Chromosomes) ATPase proteins are integral components of complexes bearing the same name, crucial for the spatial organization of DNA across diverse life forms, spanning bacteria, archaea, and eukaryotes. It is proposed that in bacteria, SMC complexes facilitate DNA compaction through loop extrusion and aid in the segregation of daughter nucleoids. In this paper, the properties of the SMC ATPase protein from Ureaplasma parvum were investigated by using a spectrum of methods, including conventional biochemical methods as well as advanced single-molecule techniques. Our findings reveal distinctive properties of this protein compared to its extensively studied homologue from Bacillus subtilis. Notably, our results suggest that U. parvum Smc ATPase facilitates DNA compaction even in the absence of ATP.

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细小脲原体SMC蛋白与DNA相互作用的特性。

SMC（染色体结构维持）atp酶蛋白是具有相同名称的复合物的组成部分，对于跨越细菌，古细菌和真核生物的各种生命形式的DNA空间组织至关重要。有人提出，在细菌中，SMC复合物通过环挤压促进DNA压实，并有助于子类核的分离。本文采用一系列方法，包括常规生化方法和先进的单分子技术，研究了小乌菌SMC atp酶蛋白的性质。我们的发现揭示了该蛋白与广泛研究的枯草芽孢杆菌同源物相比的独特特性。值得注意的是，我们的研究结果表明，即使在没有ATP的情况下，小乌菌Smc ATP酶也能促进DNA的压实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.