Fems Microbiology Letters最新文献

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Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998. 弯曲乳酸杆菌CP2998中脂磷胆酸的化学结构。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf005
Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota
{"title":"Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998.","authors":"Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota","doi":"10.1093/femsle/fnaf005","DOIUrl":"10.1093/femsle/fnaf005","url":null,"abstract":"<p><p>Latilactobacillus curvatus, found in various fermented foods, is a promising probiotic with unique health benefits. Lipoteichoic acid (LTA) is a characteristic amphiphilic surface polymer of Gram-positive bacteria and exhibits immunomodulatory activities. Despite the structural diversity of LTA among different bacterial species and strains, no information is available on the chemical structure of LTA in L. curvatus. In this study, we aimed to determine the structure of LTA isolated from L. curvatus CP2998. One- and two-dimensional nuclear magnetic resonance spectra of intact LTA revealed that LTA had a glycerolphosphate polymer as a hydrophilic main chain with partial substitutions of α-linked glucose and d-alanine at the hydroxy group at position 2 of the glycerol residue. The anchor glycolipid fraction was obtained by hydrofluoric acid treatment. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry spectrum of the anchor glycolipid revealed that it contained diglucosyldiacylglycerol and diglucosylmonoacylglycerol. Our results suggest that L. curvatus CP2998 possesses a typical type I LTA structure; however, the lactic acid bacteria-specific anchor glycolipid structures, such as tri- or tetra-saccharides and three fatty acid residues, were not identified.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Safety and amino acid profiles of Bacillus safensis in soybean fermentation. 大豆发酵中萨芬芽孢杆菌的安全性及氨基酸谱分析。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf063
Sumin Seo, Do-Won Jeong, Sooyoung Sul, Jong-Hoon Lee
{"title":"Safety and amino acid profiles of Bacillus safensis in soybean fermentation.","authors":"Sumin Seo, Do-Won Jeong, Sooyoung Sul, Jong-Hoon Lee","doi":"10.1093/femsle/fnaf063","DOIUrl":"10.1093/femsle/fnaf063","url":null,"abstract":"<p><p>This study evaluated the antibiotic resistance, toxigenic potential, and amino acid (AA) production profiles of four Bacillus safensis strains in soybean fermentation. Minimum inhibitory concentration tests revealed intrinsic resistance to chloramphenicol and clindamycin, supported by the chromosomal presence of the catA gene. No other acquired resistance genes were identified. Virulence genes associated with foodborne illness in Bacillus cereus-including nhe, hbl, cytK, and ces-were not detected in any strain, confirming the absence of typical toxin-associated determinants. Analysis of free AAs revealed that all B. safensis strains significantly increased levels of 15 proteinogenic AAs, including all nine essential AAs, in soybean cultures. Principal component analysis of 22 AAs demonstrated that B. safensis strains, especially KGU003, induced characteristic increases in alanine, glutamic acid, glutamine, and γ-aminobutyric acid (GABA). Notably, three of the four strains produced higher levels of GABA than Bacillus velezensis DMB06, a known high GABA producer, with KGU003 generating over 3-fold more. These results indicate that B. safensis strains meet core safety criteria and possess distinctive AA production profiles. Therefore, B. safensis represents a strong candidate as a safe and functional starter culture for developing fermented soybean foods with enhanced nutritional and functional properties.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unification of symbiotic bacteria during larva-to-adult transition in Culicoides circumscriptus (Diptera: Ceratopogonidae). 边界库蠓幼虫向成虫转变过程中共生细菌的统一(双翅目:蠓科)
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf069
Ryo Ozuru, Junya Yamagishi, Ayumi Takeuchi, Yusuke Date, Takatoshi Fujii, Chihiro Sugimoto, Chie Nakajima, Yasuhiko Suzuki, Kaoru Aoki, Jun Fujii, Takashi Matsuba
{"title":"Unification of symbiotic bacteria during larva-to-adult transition in Culicoides circumscriptus (Diptera: Ceratopogonidae).","authors":"Ryo Ozuru, Junya Yamagishi, Ayumi Takeuchi, Yusuke Date, Takatoshi Fujii, Chihiro Sugimoto, Chie Nakajima, Yasuhiko Suzuki, Kaoru Aoki, Jun Fujii, Takashi Matsuba","doi":"10.1093/femsle/fnaf069","DOIUrl":"10.1093/femsle/fnaf069","url":null,"abstract":"<p><p>Blood-sucking midges such as Leptoconops and Culicoides are of medical importance due to their role in causing skin irritation and potentially transmitting pathogens. Investigating their bacterial communities, including possible endosymbionts, may help clarify ecological adaptations and interactions with hosts. Leptoconops nipponensis Tokunaga (Lnt) and Culicoides circumscriptus (Cc), blood-sucking midges, cause severe itching and inflammation in humans. Cc was collected from a small sample of an outbreak swarm of Lnt in the peninsula area of Yonago City, Tottori Prefecture, Japan. This study compared the bacterial flora of Lnt and Cc, revealing distinct bacterial diversity shifts in these insect species between life stages. We analyzed the bacterial communities of adult and larval females of Cc and Lnt using MiSeq sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Notably, alpha diversity in Cc adults was significantly reduced to 1.5 (n = 43), indicating that Cc adults were dominated by a single bacterial genus, compared to 14.9 in Cc larvae (n = 19). BLAST (Basic Local Alignment Search Tool) analysis identified this dominant genus in adult Cc as Rickettsia (Candidatus Tisiphisa), which is known for transovarial transmission in arthropod vectors. In contrast, the bacterial diversity of Lnt showed no significant difference between adults (18.1, n = 32) and larvae (n = 15). These findings suggest that the dominance of Rickettsia in Cc (Candidatus Tisiphisa) adults is linked to their emergence, potentially reflecting differences in reproductive biology and ecological adaptations between these two insect species. Further research is needed to elucidate the functional role of Rickettsia in the life cycle and physiology of Cc.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenotyping as a tool to study the impact of seed priming and arbuscular mycorrhizal fungi on tomato response to water limitation. 以表型分析为工具,研究种子萌发和丛枝菌根真菌对番茄水分限制反应的影响。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf064
Luca Giovannini, Pierpaolo Del Boccio, Chiara Pagliarani, Walter Chitarra, Adriano Conte, Vincenzo Montesano, Angelo Petrozza, Stephan Summerer, Francesco Cellini, Eva Cañizares, Alexandros Spanos, Francesco Bergese, Fabiano Sillo, Marzia Vergine, Federico Vita, Silvia De Rose, Miguel González-Guzmán, Vasileios Fotopoulos, Vicent Arbona, Raffaella Balestrini
{"title":"Phenotyping as a tool to study the impact of seed priming and arbuscular mycorrhizal fungi on tomato response to water limitation.","authors":"Luca Giovannini, Pierpaolo Del Boccio, Chiara Pagliarani, Walter Chitarra, Adriano Conte, Vincenzo Montesano, Angelo Petrozza, Stephan Summerer, Francesco Cellini, Eva Cañizares, Alexandros Spanos, Francesco Bergese, Fabiano Sillo, Marzia Vergine, Federico Vita, Silvia De Rose, Miguel González-Guzmán, Vasileios Fotopoulos, Vicent Arbona, Raffaella Balestrini","doi":"10.1093/femsle/fnaf064","DOIUrl":"10.1093/femsle/fnaf064","url":null,"abstract":"<p><p>This study explores the effects of natural seed priming compounds (i.e. chitosan alone and in combination with salicylic acid or melatonin) with the symbiosis of arbuscular mycorrhizal fungi (AMF) on the capability of two Italian tomato varieties (Principe Borghese and San Marzano nano) to withstand water deprivation through high-throughput plant phenotyping technology. Plant responses have been automatically evaluated by integrating physiological, morpho-biometric, and biochemical data. Under water deprivation, AMF-inoculated plants exhibited enhanced physiological performance, by reducing oxidative damage and improving stomatal function. Digital phenotyping provides a non-invasive approach to assess the effects of external factors, such as the impact of mycorrhizal fungi on plant development. RGB (visible light) imaging enables the analysis of morphological traits like plant size and growth patterns, and of colorimetric changes used as a proxy of physiological responses. Biochemical analyses revealed increased carotenoid and flavonoid content in chitosan + salicylic acid-treated plants with AMF, particularly in Principe Borghese. Genotype-dependent differences were evident in terms of fruit production, where Principe Borghese plants showed significantly more red fruits in presence of AM fungus. The results underline the potential of combined AMF and natural compound application as a sustainable strategy for improving tomato resilience to water stress, contributing to resource-efficient agricultural practices and climate change mitigation.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144495427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prolonged anoxic exposure impacts antibiotic sensitivity profiles of Pseudomonas aeruginosa. 长期缺氧暴露影响铜绿假单胞菌的抗生素敏感性。
IF 2.2 4区 生物学
Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf066
Maik Kok, Wisse van Os, Thomas Hankemeier, J G Coen van Hasselt
{"title":"Prolonged anoxic exposure impacts antibiotic sensitivity profiles of Pseudomonas aeruginosa.","authors":"Maik Kok, Wisse van Os, Thomas Hankemeier, J G Coen van Hasselt","doi":"10.1093/femsle/fnaf066","DOIUrl":"10.1093/femsle/fnaf066","url":null,"abstract":"<p><p>Chronic respiratory tract infections with Pseudomonas aeruginosa frequently occur in patients with cystic fibrosis, chronic obstructive pulmonary disease, and bronchiectasis. A hallmark of these conditions is the accumulation of mucus plugs, creating oxygen-limited niches. Within these microenvironments, P. aeruginosa undergoes cellular modifications that may alter its antibiotic sensitivity. Although the acute effects of anoxia are well studied, the impact of prolonged anoxic exposure on antibiotic sensitivity remains unclear. In this study, we developed anoxic-conditioned P. aeruginosa strains by passaging a laboratory strain for 22 days in an anoxic environment. We performed time-kill assays with both parental and anoxic-conditioned strains in anoxic and aerobic environments, using ceftazidime, ciprofloxacin, colistin, and tobramycin. The anoxic-conditioned strains exhibited increased susceptibility to tobramycin and reduced sensitivity to colistin and ceftazidime. These differences were attributed to altered killing rates (as with tobramycin) or reduced regrowth under anoxic conditions (as with colistin). For ciprofloxacin, a steeper killing rate was observed against the anoxic-conditioned strains, but 24-h outcomes were similar to the parental strain. Overall, our findings demonstrate that long-term anoxia alters antibiotic sensitivity in P. aeruginosa differently than acute anoxia, with important implications for treating chronic infections in oxygen-limited environments.</p>","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice 耐多药金黄色葡萄球菌感染噬菌体的分离和特性鉴定及其在小鼠中的治疗用途
IF 2.1 4区 生物学
Fems Microbiology Letters Pub Date : 2024-09-13 DOI: 10.1093/femsle/fnae072
Zhen Xiao, Hongyi Xu, Juan Wang, Xueyuan Hu, Xiumei Huang, Shiping Song, Qingqing Zhang, Yanxin Liu, Yaopeng Liu, Na Liu, Junhui Liu, Ge Zhao, Xiyue Zhang, Yuehua Li, Jianmei Zhao, Junwei Wang, Huanqi Liu, Lin Wang, Zhina Qu
{"title":"Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice","authors":"Zhen Xiao, Hongyi Xu, Juan Wang, Xueyuan Hu, Xiumei Huang, Shiping Song, Qingqing Zhang, Yanxin Liu, Yaopeng Liu, Na Liu, Junhui Liu, Ge Zhao, Xiyue Zhang, Yuehua Li, Jianmei Zhao, Junwei Wang, Huanqi Liu, Lin Wang, Zhina Qu","doi":"10.1093/femsle/fnae072","DOIUrl":"https://doi.org/10.1093/femsle/fnae072","url":null,"abstract":"In recent years, the emergence of multidrug-resistant bacteria has limited the selection of drugs for treating bacterial infections, reduced clinical efficacy, and increased treatment costs and mortality. It is urgent to find alternative antibiotics. In order to explore a new method for controlling methicillin resistant Staphylococcus aureus (S. aureus) , this study isolated and purified a multi drug resistant S. aureus broad-spectrum phage JPL-50 from wastewater. JPL-50 belongs to the Siphoviridae family after morphological observation, biological characterization, and transmission electron microscopy (TEM) fragmentation spectrum analysis. It can cleave 84% of tested S. aureus (168/200) , in which 100% of tested mastitis-associated strains (48/48) and 72.04% of MRSA strains (67/93) were lysed. In addition, it has an optimal growth temperature of about 30°C, a high activity within a wide pH range (pH 3–10) , and an optimal multiplicity of infection of 0.01. The one-step growth curve shows a latent time of 20 minutes, an explosive time of 80 minutes. JPL-50 was 16, 927 bp in length and was encoded by double-stranded DNA, with no genes associated with bacterial resistance or virulence factors detected. In a therapeutic study, injection of the phage JPL-50 once and for 7 times in 7 days protected 40% and 60% of the mice from fatal S.aureus infection, respectively. More importantly, JPL-50-doxycycline combination could effectively inhibit host S.aureus in vitro and reduce the use of doxycycline within 8 hours. In conclusion, the bacteriophage JPL-50 has a wide lysis spectrum, high lysis rate, high tolerance to extreme environments, and moderate in vivo activity, providing ideas for developing multidrug-resistant S. aureus infections.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"65 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Organization, Conservation, and Diversity of Biosynthetic Gene Clusters in Bacillus sp. BH32 and Its Closest Relatives in the Bacillus cereus Group 蜡样芽孢杆菌 BH32 及其近亲中生物合成基因簇的组织、保存和多样性
IF 2.1 4区 生物学
Fems Microbiology Letters Pub Date : 2024-09-09 DOI: 10.1093/femsle/fnae071
Hadj Ahmed Belaouni, Amine Yekkour, Abdelghani Zitouni, Atika Meklat
{"title":"Organization, Conservation, and Diversity of Biosynthetic Gene Clusters in Bacillus sp. BH32 and Its Closest Relatives in the Bacillus cereus Group","authors":"Hadj Ahmed Belaouni, Amine Yekkour, Abdelghani Zitouni, Atika Meklat","doi":"10.1093/femsle/fnae071","DOIUrl":"https://doi.org/10.1093/femsle/fnae071","url":null,"abstract":"This study explores the organization, conservation, and diversity of biosynthetic gene clusters (BGCs) among Bacillus sp. strain BH32, a plant-beneficial bacterial endophyte, and its closest non-type Bacillus cereus group strains. BGC profiles were predicted for each of the 17 selected strains using antiSMASH, resulting in the detection of a total of 198 BGCs. We quantitatively compared the BGCs and analyzed their conservation, distribution, and evolutionary relationships. The study identified both conserved and singleton BGCs across the studied Bacillus strains, with minimal variation, and discovered two major BGC synteny blocks composed of homologous BGCs conserved within the B. cereus group. The identified BGC synteny blocks provide insight into the evolutionary relationships and diversity of BGCs within this complex group.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"23 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142223408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dynamic responses of Salmonella Typhimurium to re-exposure to sublethal ciprofloxacin 鼠伤寒沙门氏菌对再次接触亚致死环丙沙星的动态反应
IF 2.1 4区 生物学
Fems Microbiology Letters Pub Date : 2024-06-27 DOI: 10.1093/femsle/fnae050
Jiseok Yi, Junhwan Kim, Juhee Ahn
{"title":"Dynamic responses of Salmonella Typhimurium to re-exposure to sublethal ciprofloxacin","authors":"Jiseok Yi, Junhwan Kim, Juhee Ahn","doi":"10.1093/femsle/fnae050","DOIUrl":"https://doi.org/10.1093/femsle/fnae050","url":null,"abstract":"This study was designed to evaluate the history-dependent behaviors of Salmonella Typhimurium re-exposed to sublethal levels of ciprofloxacin. The S. Typhimurium cells were pre-exposed to 0 (CON), 1/16 (LOW), 1/8 (MED), and 1/4 (HIGH) MICs of ciprofloxacin, followed by re-exposure to the same concentrations. The bacterial growth, post-antibiotic effect (PAE), relative fitness, and swimming motility of treatments were evaluated in the absence of ciprofloxacin. The lag phase duration (LPD) was estimate to assess bacterial recovery under ciprofloxacin exposure. A disk diffusion assay was used to determine the cross-resistance and collateral sensitivity of CON, LOW, MED, and HIGH treatments to ciprofloxacin (CIP), ceftriaxone (CEF), erythromycin (ERY), gentamicin (GEN), and polymyxin B (POL). The S. Typhimurium cells pre-exposed to ciprofloxacin were susceptible in antibiotic-free media, showing delayed growth. The highest PAE (&amp;gt; 1 h) and bacterial fluctuation (CV = 5%) were observed at the High treatment compared to the CON. The HIGH treatment had the lowest relative fitness levels (0.87) and swimming motility (55 mm). The LPD was significantly decreased at the LOW treatment (1.8 h) when re-exposed to 1/16× MIC of ciprofloxacin. The LOW, MED, and HIGH treatments showed the cross-resistance to POL and the collateral sensitivity to CEF, ERY, and GEN. The pre-exposure to ciprofloxacin could induce phenotypic diversity, corresponding to the history-dependent behaviors. These results provide important insights for the dynamic nature of bacterial populations when re-exposed to sublethal concentrations of antibiotics.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"140 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141502758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of PetrifilmTM AC and Pour plate techniques used for the heterotrophic aerobic bacterial count in water 用于水中异养需氧细菌计数的 PetrifilmTM AC 与倒平板技术的比较
IF 2.1 4区 生物学
Fems Microbiology Letters Pub Date : 2024-04-30 DOI: 10.1093/femsle/fnae029
Faith Mkhwanazi, Tshilidzi Mazibuko, Olivia Mosoma, Malefaso Rathebe, Mrudula Patel
{"title":"Comparison of PetrifilmTM AC and Pour plate techniques used for the heterotrophic aerobic bacterial count in water","authors":"Faith Mkhwanazi, Tshilidzi Mazibuko, Olivia Mosoma, Malefaso Rathebe, Mrudula Patel","doi":"10.1093/femsle/fnae029","DOIUrl":"https://doi.org/10.1093/femsle/fnae029","url":null,"abstract":"Heterotrophic bacteria (HPC) are commonly found in water samples. While these bacterial counts do not necessarily indicate a health hazard, high counts provide a good indication of the efficiency of water disinfection and integrity of distribution systems. The aim of this study was to compare the PetrifimTM AC method to the Pour Plate technique for the testing of HPC in water samples. Artificially contaminated (192 samples) and natural water samples (25) were processed using two methods. Both methods accurately detected high, medium and low counts of HPC, producing average Z scores between -2 and + 2. Paired-wise student t-test and correlation coefficient showed nonsignificant differences between the results of two methods. Acceptable repeatability and reproducibility was obtained using both the methods. Uncertainty of measurement for PetrifilmTM AC and Pour Plate method was found to be 2.9% and 5.4% respectively. PetrifilmTM AC proved to be robust at 33 °C and 37 °C. In conclusion, PetrifimTM AC, which is easy to process, read and less time consuming, proved to be comparable to the conventional Pour Plate method in establishing HPC in water. In addition, PetrifimTM AC requires less space for the processing and incubation, generate small volume of waste for disposal and requires no equipment, except for the incubator.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"56 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and characterization of a novel type of ketohexokinase from the haloarchaeon Haloferax volcanii 卤虫 Haloferax volcanii 中一种新型酮异酵素酶的鉴定和表征
IF 2.1 4区 生物学
Fems Microbiology Letters Pub Date : 2024-04-08 DOI: 10.1093/femsle/fnae026
Marius Ortjohann, Peter Schönheit
{"title":"Identification and characterization of a novel type of ketohexokinase from the haloarchaeon Haloferax volcanii","authors":"Marius Ortjohann, Peter Schönheit","doi":"10.1093/femsle/fnae026","DOIUrl":"https://doi.org/10.1093/femsle/fnae026","url":null,"abstract":"Ketohexokinase (KHK) catalyzes the ATP-dependent phosphorylation of fructose, forming fructose-1-phosphate and ADP. The enzyme is well studied in Eukarya, in particular in human and other vertebrates, but homologs have not been identified in Bacteria and Archaea. Here we report the identification of a novel type of KHK from the haloarchaeon Haloferax volcanii (HvKHK). The encoding gene khk was identified as HVO_1812. The gene was expressed as 90 kDa homodimeric protein, catalyzing the phosphorylation of fructose with a Vmax value of 59 U/mg and apparent KM values for ATP and fructose of 0.47 mM and 1.29 mM, respectively. Homologs of HvKHK were only identified in few haloarchaea and halophilic Bacteria. The protein showed low sequence identity to characterized KHKs from eukaryotes and phylogenetic analyses indicate that haloarchaeal KHKs are largely separated from eukaryal KHKs. This is the first report of the identification of KHKs in prokaryotes that form a novel cluster of sugar kinases within the ribokinase/pfkB superfamily.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"84 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140583638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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