Fems Microbiology Letters最新文献_第9页

High prevalence of antibiotic-resistant Escherichia coli harbouring extended-spectrum beta-lactamase (ESBL) in raw chicken meat sold at retail in Klang Valley, Malaysia. 马来西亚巴生谷零售的生鸡肉中含有广谱β -内酰胺酶（ESBL）的耐抗生素大肠杆菌的高发率

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf039

Sur Guat Goh, Son Radu, Yuli Haryani, Lee Epeng, Mahmud Ab Rashid Nor-Khaizura, Noor Azira Abdul-Mutalib, Hanan Hasan

{"title":"High prevalence of antibiotic-resistant Escherichia coli harbouring extended-spectrum beta-lactamase (ESBL) in raw chicken meat sold at retail in Klang Valley, Malaysia.","authors":"Sur Guat Goh, Son Radu, Yuli Haryani, Lee Epeng, Mahmud Ab Rashid Nor-Khaizura, Noor Azira Abdul-Mutalib, Hanan Hasan","doi":"10.1093/femsle/fnaf039","DOIUrl":"10.1093/femsle/fnaf039","url":null,"abstract":"Chicken is the most widely consumed meat in Malaysia as it is abundant, provides good nutrient and taste, and available at an affordable price. However, it is known to harbour various foodborne pathogens including faecal microorganism, Escherichia coli. There are various routes and factors that can cause contamination of E. coli in chicken. Furthermore, numerous reports have shown that over the past decades, the trends of antimicrobial resistance among foodborne pathogens have been increasing rapidly. Therefore, the present work aimed to assess the prevalence of E. coli contamination by examining various contributing factors and its antibiotic resistance in raw chicken meat sold in Klang Valley, Malaysia. Results showed that 74% of the samples were contaminated with E. coli with wet markets showing higher prevalence (17%) of E. coli than in hypermarkets. Univariate analysis within the same risk factor showed that packaging process, storage temperature, and antibiotics had significant effects on the prevalence of E. coli (∼ 6.097 log CFU/g). The E. coli loads were significantly influenced by market type and storage temperature as validated by Mann-Whitney tests. All E. coli isolates displayed multiple antibiotic resistance (MAR) index ranging from 0.33 to 1.00, and 35 E. coli isolates showed the highest MAR index (1.00), being resistant to 12 antibiotics. Furthermore, 90% of E. coli isolates contained extended-spectrum beta-lactamase genotypes that can subvert potent antibiotic, beta-lactam. The findings from the present work would help reduce the risk of foodborne illnesses by identifying the risk factors associated with E. coli prevalence in chicken and provide the basis to revise guidelines on antibiotic use in livestock to reduce antimicrobial resistance.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial susceptibility and genomic characterization of Vibrio cholerae non-O1/non-O139 isolated from clinical and environmental samples in Jiaxing City, China. 嘉兴市临床和环境中分离的非o1 /非o139型霍乱弧菌的药敏及基因组特征

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf009

Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen

{"title":"Antimicrobial susceptibility and genomic characterization of Vibrio cholerae non-O1/non-O139 isolated from clinical and environmental samples in Jiaxing City, China.","authors":"Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen","doi":"10.1093/femsle/fnaf009","DOIUrl":"10.1093/femsle/fnaf009","url":null,"abstract":"Non-O1/non-O139 (NOVC) strains inhabit aquatic environments and sporadically induce human illnesses. This study involved the virulence and antimicrobial genetic characterization of 176 NOVC strains, comprising 25 from clinical samples and 151 from environmental sources, collected between 2021 and 2023. The antimicrobial susceptibility of the examined NOVC population was predominantly high, exhibiting only poor susceptibility to colistin, with 89.2% resistance. The examination of virulence genes revealed that the majority of strains were positive for glucose metabolism (als gene) (169/176, 96.0%). Through multilocus sequence typing, the 176 NOVC strains were categorised into 121 sequence types, 79 of which were novel. NOVC strains demonstrate significant genetic variability and frequently engage in recombination. This work offers genetic characterization of the pathogenicity and antimicrobial resistance of a NOVC community. Our findings offer insights that may aid in the development of preventative and treatment methods for this pathogen.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seven years later: native AMF inoculation improves grassland successional stage, floristic quality index, and diversity, while suppressing weeds. 7年后：原生AMF接种改善了草地演替阶段、植物区系质量指数和多样性，同时抑制了杂草。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf079

Liz Koziol, Thomas P McKenna, Eric B Duell, James D Bever

{"title":"Seven years later: native AMF inoculation improves grassland successional stage, floristic quality index, and diversity, while suppressing weeds.","authors":"Liz Koziol, Thomas P McKenna, Eric B Duell, James D Bever","doi":"10.1093/femsle/fnaf079","DOIUrl":"10.1093/femsle/fnaf079","url":null,"abstract":"Reintroducing key soil microbes, such as arbuscular mycorrhizal (AM) fungi, can enhance prairie restoration, but the long-term persistence of these effects is unclear. In 2017, a tallgrass prairie restoration experiment was established that applied an inoculation gradient of native AM fungal inoculant ranging from 0 to 8192 kg/ha. Here, we sample the resulting prairie community establishment and spread seven growing seasons later (72 months). We found that AM fungal application increased total diversity, floristic quality index, and average coefficient of conservatism scores-metrics that assess an area's ecological integrity based on plant species. Moreover, high application densities of native AM fungi reduced non-native plant abundance. Furthermore, inoculated prairie plots were more likely to extend 1.5 m into adjacent nonseeded old-field plots, enhancing native diversity and reducing invasion in marginal lands. These findings highlight the lasting benefits of native AM fungal inoculation in prairie restoration and their potential to facilitate long-term ecosystem recovery, aligning with global biodiversity conservation efforts such as the UN Sustainable Development Goal 15 (Life on Land). Given that land managers spend ~25% of their time and considerable economic input managing invasive species, these findings demonstrate that the reintroducing native AM fungi from reference ecosystems during grassland restoration could provide a cost-effective means of enhancing ecological outcomes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144774978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leveraging networks to provide guidance to early-career microbiologists: the Australian Society for Microbiology Mentoring Program. 利用网络为早期职业微生物学家提供指导：澳大利亚微生物学会指导计划。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf065

Priscilla Johanesen, Mark A Schembri, Dena Lyras, Sam Manna

引用次数: 0

Metabolic analysis of the mode of action and mode of resistance of fusidic acid against Staphylococcus aureus. 氟西地酸对金黄色葡萄球菌作用模式（MoA）和耐药模式（MoR）的代谢分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf011

Dan Luo, Juanjuan Ma, Weile Xie, Zhe Wang

{"title":"Metabolic analysis of the mode of action and mode of resistance of fusidic acid against Staphylococcus aureus.","authors":"Dan Luo, Juanjuan Ma, Weile Xie, Zhe Wang","doi":"10.1093/femsle/fnaf011","DOIUrl":"10.1093/femsle/fnaf011","url":null,"abstract":"Understanding bacterial responses to antibiotics is essential for identifying resistance mechanisms and developing novel therapies. This study evaluated the resistance of Staphylococcus aureus (S. aureus) to fusidic acid (FD) in 100 patients with skin and soft tissue infections (SSTIs), revealing susceptibility to FD despite resistance to other antibiotics. Through adaptive laboratory evolution, we developed a highly FD-resistant strain, E10, and identified three gene mutations (fusA, BPENGOFF-00211, and rplF) using whole-genome sequencing. The fusA mutation was the primary contributor to resistance. Furthermore, the evolved fusA mutant strain (H457Y) displayed impaired coagulation function and reduced growth rates. We also analyzed the metabolomic profiles of ancestral ATCC 25923 and evolved E10 strains, both treated and untreated with FD, revealing that the fusA gene can independently induce metabolic reprogramming. These changes primarily impacted pathways involved in central carbon metabolism, nucleotide metabolism, and amino acid synthesis. This study highlights the complexity of FD resistance in S. aureus and offers insights into the metabolic pathways associated with antibiotic resistance.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodotorula mucilaginosa (A. Jörg.) F.C. Harrison 1928 and onychomycosis: three case reports for an unusual and underestimated combo. 粘液红霉菌（A. Jörg.）1928年哈里森和甲癣：三例报告为一个不寻常的和低估的组合。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf089

Mirko Benvenuti, Martina Burlando, Emanuele Claudio Cozzani

引用次数: 0

Understanding the role of the cytadhesins on the pathogenesis and transmission of Mycoplasma genitalium infection: a minireview. 了解细胞粘附素在生殖道支原体感染的发病机制和传播中的作用：综述。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf099

Miguel Fernández-Huerta, Zaira Moure, Berta Fidalgo, Mateu Espasa

{"title":"Understanding the role of the cytadhesins on the pathogenesis and transmission of Mycoplasma genitalium infection: a minireview.","authors":"Miguel Fernández-Huerta, Zaira Moure, Berta Fidalgo, Mateu Espasa","doi":"10.1093/femsle/fnaf099","DOIUrl":"10.1093/femsle/fnaf099","url":null,"abstract":"First isolated in 1981, Mycoplasma genitalium is now well recognized as a frequent cause of urethritis and several other urogenital syndromes in both men and women. The cytoadhesins P110 (MgpC) and P140 (MgpB), located in the terminal organelle of the bacterium, mediate the establishment and persistence of M. genitalium infection in the reproductive tract tissues. This adhesion complex exposes a large extracellular domain, mediating bacterial adhesion to the anogenital tissue. The antigenic variation promoted by reciprocal recombination contribute to the efficient colonization of the urogenital environment and the immune evasion strategy of the bacterium. Although M. genitalium was traditionally detected in ano-genital samples, sexual transmission was not postulated until the early 2000s. Now, it is well accepted that M. genitalium can lead to a sexually transmitted infection. While vaginal sex may constitute the primary transmission route in terms of risk, anal intercourse in men who have sex with men is likely the most common cause of spread. Acknowledging the exquisite adaptation of the bacterium to diverse ecological niches in the human body, the unique features of the proteins P110 and P140 may influence transmission, dissemination and disease.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial activity of partially purified polysaccharide extract of Arthrospira platensis PCST5. platarthrospira PCST5部分纯化多糖提取物的抑菌活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf082

Tahereh Hosseinabadi, Zahra Zare Mehrjardy, Arash Mahboubi, Maryam Tabarzad

{"title":"Antimicrobial activity of partially purified polysaccharide extract of Arthrospira platensis PCST5.","authors":"Tahereh Hosseinabadi, Zahra Zare Mehrjardy, Arash Mahboubi, Maryam Tabarzad","doi":"10.1093/femsle/fnaf082","DOIUrl":"10.1093/femsle/fnaf082","url":null,"abstract":"Arthrospira platensis (Spirulina) is one of the highly valuable cyanobacteria in the food and pharmaceutical industries. The intracellular and extracellular polysaccharide (PS) extracts of A. platensis have been exhibited different biological functions. Here, the antibacterial and antifungal activity of PS extract from Arthrospira platensis PCST5 was studied. Arthrospira platensis PCST5 was cultured in Zarouk medium for 25 days. Then, the polysaccharide content of biomass and excreted metabolites was extracted. The antibacterial and antifungal activity of samples was assessed. The yield of PS extraction was around 8.5% and 11.5% from biomass and excreted metabolites, respectively. The most potent antibacterial activity was for sulfuric acid-treated insoluble polysaccharide fraction, against Staphylococcus aureus (MIC = 4.25 µg ml-1). In addition, the highest antifungal activity was observed for crude excreted metabolites against Aspergillus niger (MIC = 15.625 µg ml-1) and for PS extract and sulfuric acid treated insoluble polysaccharide fraction against Candida albicans (MIC = 15.625 and 0.531 µg ml-1, respectively). This antimicrobial activity of polysaccharide extract against S. aureus can be valued in the development of topical products for wound healing. Further studies should be performed to determine the exact structure of active component and to evaluate other potential pharmacological activities in this regard.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-directed disruption of wc-2 leads to the absence of fruiting body development in Pleurotus ostreatus. CRISPR/ cas9定向破坏wc-2导致平菇子实体发育缺失。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf104

Godai Toyonishi, Takehito Nakazawa, Daishiro Koshi, Masato Horii, Gi Hong An, Moriyuki Kawauchi, Yoichi Honda

引用次数: 0

Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria. 一种来自山地湿地的嗜中性、降解木聚糖的植物菌——石勒斯里亚。石勒斯里亚属的修正描述。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf006

Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh

{"title":"Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria.","authors":"Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh","doi":"10.1093/femsle/fnaf006","DOIUrl":"10.1093/femsle/fnaf006","url":null,"abstract":"Aerobic chemoorganotrophic planctomycetes of the genus Schlesneria colonize a wide spectrum of freshwater ecosystems. The only described species of this genus, S. paludicola, is represented by ellipsoid-shaped, moderately acidophilic bacteria isolated from acidic peat bogs. Here, we characterize a novel neutrophilic member of this genus from a mountain wetland, strain T3-172T. This isolate possesses unpigmented, non-motile, spherical cells, which grow on media containing some mono- and polysaccharides in the pH range of 6.0-7.5 and at temperatures between 15 and 37°C. Good growth is observed on xylan, one of the major plant cell wall biopolymers. The genome of strain T3-172T is 7.16 Mb in size and contains two unlinked sets of rRNA genes, 93 tRNA genes, and about 5500 potential protein-coding genes. Among the latter, the GH5 family protein is identified as the most reliable candidate for the cleavage of xylan. The 16S rRNA gene sequence of strain T3-172T displays 97.8% similarity to that of S. paludicola MPL7T, and the average nucleotide identity between the genomes of these planctomycetes is 71.6%. We, therefore, propose a novel species of the genus Schlesneria, S. sphaerica sp. nov., with strain T3-172T (=KCTC 102306T = VKM B-3856T) as the type strain.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0