Fems Microbiology Letters最新文献_第9页

Tryptophan transport gene inactivation promotes the development of antibiotic resistance in Escherichia coli. 色氨酸转运基因失活促进了大肠杆菌抗生素耐药性的产生。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae057

Beibei Hou, Jianxiao Song, Huan Wang, Nan Ye, Rui-Wu Wang

{"title":"Tryptophan transport gene inactivation promotes the development of antibiotic resistance in Escherichia coli.","authors":"Beibei Hou, Jianxiao Song, Huan Wang, Nan Ye, Rui-Wu Wang","doi":"10.1093/femsle/fnae057","DOIUrl":"10.1093/femsle/fnae057","url":null,"abstract":"Indole serves as a signaling molecule that could regulate different bacterial physiological processes, including antibiotic resistance through biofilm formation and drug efflux pump activity. In Escherichia coli, indole is produced through the tryptophan pathway, which involves three permeases (Mtr, AroP, and TnaB) that can transport the amino acid tryptophan. Although these permeases play distinct roles in the secretion of indole biosynthesis, their impact on multidrug resistance mediated by indole remaines unclear. This study was designed to investigate the connection between the tryptophan transport system and antibiotic resistance by constructing seven gene deletion mutants from E. coli MG1655 (wild type). Our result showed that deletion of the aroP or tnaB gene led to increased antibiotic resistance as evaluated by MICs for different antibiotics. Efflux activity test results revealed that the increased antibiotic resistance was related with the AcrAB-Tolc drug efflux pump in the mutants. The transcriptome analysis further demonstrated that decreased susceptibility to kanamycin and ampicillin in E. coli was accompanied by reduced accumulation of reactive oxygen species and decreased motility. These findings highlight the substantial influence of the tryptophan transport system on antibiotic resistance in E. coli, which is crucial for developing strategies against antibiotic resistance in bacterial infections.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenemase and extended-spectrum beta-lactamase-producing bacteria in waters originating from a single landfill in Slovenia. 斯洛文尼亚单一垃圾填埋场水域中产生碳青霉烯酶和广谱β-内酰胺酶的细菌。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae070

Valerija Tkalec, Polona Lindic, Tatjana Jursa, Hermina Ivanusa Sket, Leon Maric, Mojca Cimerman, Maja Rupnik, Andrej Golle

{"title":"Carbapenemase and extended-spectrum beta-lactamase-producing bacteria in waters originating from a single landfill in Slovenia.","authors":"Valerija Tkalec, Polona Lindic, Tatjana Jursa, Hermina Ivanusa Sket, Leon Maric, Mojca Cimerman, Maja Rupnik, Andrej Golle","doi":"10.1093/femsle/fnae070","DOIUrl":"10.1093/femsle/fnae070","url":null,"abstract":"Groundwater, rainwater, and leachate associated with a single landfill were analysed to detect extended-spectrum beta-lactamase (ESBL)-producing and carbapenemase (CP)-producing bacteria. After cultivation on three commercial selective-differential media, 240 bacterial isolates were obtained and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Isolates from clinically relevant species were further genotyped by enterobacterial repetitive intergenic consensus polymerase chain reaction, and tested for antibiotic susceptibility and presence of CPs and ESBL enzymes. Two ESBL-producing isolates and two isolates producing CPs were detected in rainwater, groundwater, and leachate: Klebsiella oxytoca complex with the gene for the ESBL enzyme CTX-M-1 and the gene for the CP OXA-48, Serratia fonticola with the gene for the ESBL enzyme FONA-2, and Pseudomonas aeruginosa with the gene coding Verona integron-encoded Metallo-beta-lactamases (VIM) metallo-beta-lactamase. Our study indicates that bacteria with ESBL and CP genes can be present in landfill-associated waters.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinguishing Gram-positive and Gram-negative bloodstream infections through leukocytes, C-reactive protein, procalcitonin, and D-Dimer: an empirical antibiotic guidance. 通过白细胞、C 反应蛋白、降钙素原和 D-二聚体区分革兰氏阳性和革兰氏阴性血流感染：经验性抗生素指南。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae091

Jiru Li, Hao Xia

{"title":"Distinguishing Gram-positive and Gram-negative bloodstream infections through leukocytes, C-reactive protein, procalcitonin, and D-Dimer: an empirical antibiotic guidance.","authors":"Jiru Li, Hao Xia","doi":"10.1093/femsle/fnae091","DOIUrl":"10.1093/femsle/fnae091","url":null,"abstract":"This retrospective study aimed to compare the difference of the levels of white blood cells (WBC), C-reactive protein (CRP), procalcitonin, and D-Dimer in the bloodstream infection (BSI) patients, and their values in distinguishing bacterial categories. A total of 847 BSI patients were analysed and divided into Gram-positive BSI (GP-BSI) and Gram-negative BSI (GN-BSI) groups. Most frequently isolated pathogens in GP-BSI were Staphylococcus epidermidis (35.75%), followed by Staphylococcus hominis (18.33%), and Streptococcus haemolyticus (10.16%), while in GN-BSI, Escherichia coli (30.07%), Klebsiella pneumoniae (23.98%), and Acinetobacter baumannii (13.18%) were the most common. The predictive value was evaluated based on 3 years of patient data, which showed an area under the curve (AUC) of 0.828. It was further validated using 2 years of data, which yielded an AUC of 0.925. Significant differences existed in the procalcitonin, D-Dimer, and CRP levels between GN-BSI and GP-BSI. The current results provide a more effective strategy for early differential diagnosis in bacterial categorization of BSI when combining WBC, CRP, procalcitonin, and D-Dimer measurements.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fumigation with dimethyl trisulfide to inhibit Aspergillus flavus growth, aflatoxin B1 production and virulence. 用二甲基三硫熏蒸抑制黄曲霉生长、黄曲霉毒素 B1 的产生和毒力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae102

Mingguan Yang, Honggui Lu, Nan Xiao, Yongjian Qin, Lei Sun, Rui Sun

{"title":"Fumigation with dimethyl trisulfide to inhibit Aspergillus flavus growth, aflatoxin B1 production and virulence.","authors":"Mingguan Yang, Honggui Lu, Nan Xiao, Yongjian Qin, Lei Sun, Rui Sun","doi":"10.1093/femsle/fnae102","DOIUrl":"10.1093/femsle/fnae102","url":null,"abstract":"Aspergillus flavus is a common saprophytic aerobic fungus in oil crops that poses a serious threat worldwide with the carcinogenic aflatoxin. Prevention of aflatoxin B1 contamination has great significance to ensure food safety and reduce the economic loss. The present work focuses on the antagonistic activity against A. flavus growth in peanuts by fumigation with dimethyl trisulfide. The results indicated that dimethyl trisulfide exhibits great antifungal activity against A. flavus. The conidial germination and mycelial growth of A. flavus were completely suppressed after exposure to 15 and 20 µl/l of dimethyl trisulfide, respectively. Numerous deformed conidia were found after exposure to dimethyl trisulfide at high concentration (≥20 µl/l). Scanning electron microscope observation demonstrated that dimethyl trisulfide induced severely shrinking mycelia of A. flavus. The results of OD-260 nm absorption and rhodamine-123 fluorescent staining indicated that cell membrane and mitochondria may be legitimate antifungal targets of dimethyl trisulfide. Dimethyl triethyl has a significant inhibitory effect on A. flavus infection in peanuts. In addition, dimethyl trisulfide could reduce the production of aflatoxin B1 via down-regulation of toxin synthesis and regulatory gene expression. Dimethyl trisulfide can be a tremendous potential agent for the biological control of A. flavus and deepened our understanding of the anti-fungal mechanisms of volatile organic compounds.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Second messenger c-di-AMP regulates multiple antibiotic sensitivity pathways in Mycobacterium smegmatis by discrete mechanisms. 第二信使c-di-AMP通过不同的机制调控分枝杆菌的多种抗生素敏感性途径。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae084

Aditya Kumar Pal, Dipankar Ghorai, Xueliang Ge, Biplab Sarkar, Amit Kumar Sahu, Vikas Chaudhary, Ruchi Jhawar, Suparna Sanyal, Mahavir Singh, Anirban Ghosh

{"title":"Second messenger c-di-AMP regulates multiple antibiotic sensitivity pathways in Mycobacterium smegmatis by discrete mechanisms.","authors":"Aditya Kumar Pal, Dipankar Ghorai, Xueliang Ge, Biplab Sarkar, Amit Kumar Sahu, Vikas Chaudhary, Ruchi Jhawar, Suparna Sanyal, Mahavir Singh, Anirban Ghosh","doi":"10.1093/femsle/fnae084","DOIUrl":"10.1093/femsle/fnae084","url":null,"abstract":"One of the debilitating causes of high mortality in the case of tuberculosis and other bacterial infections is the resistance development against standard drugs. There are limited studies so far to describe how a bacterial second messenger molecule can directly participate in distinctive antibiotic tolerance characteristics of a cell in a mechanism-dependent manner. Here we show that intracellular cyclic di-AMP (c-di-AMP) concentration can modulate drug sensitivity of Mycobacterium smegmatis by interacting with an effector protein or interfering with the 5'-UTR regions in mRNA of the genes and thus causing transcriptional downregulation of important genes in the pathways. We studied four antibiotics with different mechanisms of action: rifampicin, ciprofloxacin, erythromycin, and tobramycin and subsequently found that the level of drug sensitivity of the bacteria is directly proportional to the c-di-AMP concentration inside the cell. Further, we unraveled the underlying molecular mechanisms to delineate the specific genes and pathways regulated by c-di-AMP and hence result in differential drug sensitivity in M. smegmatis.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modular inquiry-based semester theme that integrates data science education and bioinformatics in protein structure function courses. 模块化探究式学期主题，将数据科学教育和生物信息学整合到蛋白质结构功能课程中。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae055

Zareen Amtul, Forough Firoozbakht, Iman Rezaeian, Arham A Aziz, Padmini Gehlaut

{"title":"A modular inquiry-based semester theme that integrates data science education and bioinformatics in protein structure function courses.","authors":"Zareen Amtul, Forough Firoozbakht, Iman Rezaeian, Arham A Aziz, Padmini Gehlaut","doi":"10.1093/femsle/fnae055","DOIUrl":"10.1093/femsle/fnae055","url":null,"abstract":"Background: With an exponential growth in biological data and computing power, familiarity with bioinformatics has become a demanding and popular skill set both in academia and industry. There is a need to increase students' competencies to be able to take on bioinformatic careers, to get them familiarized with scientific professions in data science and the academic training required to pursue them, in a field where demand outweighs the supply.Methods: Here we implemented a set of bioinformatic activities into a protein structure and function course of a graduate program. Concisely, students were given hands-on opportunities to explore the bioinformatics-based analyses of biomolecular data and structural biology via a semester-long case study structured as inquiry-based bioinformatics exercises. Towards the end of the term, the students also designed and presented an assignment project that allowed them to document the unknown protein that they identified using bioinformatic knowledge during the term.Results: The post-module survey responses and students' performances in the lab module imply that it furthered an in-depth knowledge of bioinformatics. Despite having not much prior knowledge of bioinformatics prior to taking this module students indicated positive feedback.Conclusion: The students got familiar with cross-indexed databases that interlink important data about proteins, enzymes as well as genes. The essential skillsets honed by this research-based bioinformatic pedagogical approach will empower students to be able to leverage this knowledge for their future endeavours in the bioinformatics field.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11339600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141626514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial diversity in a continuum from supraglacial habitats to a proglacial lake on the Tibetan Plateau. 青藏高原上从超冰川栖息地到冰川湖的连续过程中的细菌多样性。

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae021

Tingting Xing, Keshao Liu, Mukan Ji, Yuying Chen, Yongqin Liu

{"title":"Bacterial diversity in a continuum from supraglacial habitats to a proglacial lake on the Tibetan Plateau.","authors":"Tingting Xing, Keshao Liu, Mukan Ji, Yuying Chen, Yongqin Liu","doi":"10.1093/femsle/fnae021","DOIUrl":"10.1093/femsle/fnae021","url":null,"abstract":"Mountain glaciers are frequently assessed for their hydrological connectivity from glaciers to proglacial lakes. Ecological process on glacier surfaces and downstream ecosystems have often been investigated separately, but few studies have focused on the connectivity between the different glacial habitats. Therefore, it remains a limited understanding of bacterial community assembly across different habitats along the glacier hydrological continuum. In this study, we sampled along a glacial catchment from supraglacial snow, cryoconite holes, supraglacial runoff, ice-marginal moraine and proglacial lake on the Tibetan Plateau. The bacterial communities in these habitats were analyzed using high-throughput DNA sequencing of the 16S rRNA gene to determine the bacterial composition and assembly. Our results showed that each habitat hosted unique bacterial communities, with higher bacterial α-diversity in transitional habitats (e.g. runoff and ice-marginal moraine). Null model analysis indicated that deterministic processes predominantly shaped bacterial assembly in snow, cryoconite holes and lake, while stochastic process dominantly governed bacterial community in transitional habitats. Collectively, our findings suggest that local environment play a critical role in filtering bacterial community composition within glacier habitats. This study enhances our understanding of microbial assembly process in glacier environments and provides valuable insights into the factors governing bacterial community compositions across different habitats along the glacial hydrological continuum.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the life cycle of Helicobacter pylori bacteriophage KHP40 belonging to the genus Schmidvirus. 幽门螺杆菌噬菌体 KHP40（属于施米德病毒属）的生命周期分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae082

Masahiro Iwamoto, Michiko Takahashi, Hiromichi Maeda, Hiroaki Takeuchi, Jumpei Uchiyama, Takako Ujihara, Keizo Nagasaki, Kazuhiro Hanazaki, Satoru Seo, Naoya Kitamura, Tetsuya Yamamoto, Shigenobu Matsuzaki

{"title":"Analysis of the life cycle of Helicobacter pylori bacteriophage KHP40 belonging to the genus Schmidvirus.","authors":"Masahiro Iwamoto, Michiko Takahashi, Hiromichi Maeda, Hiroaki Takeuchi, Jumpei Uchiyama, Takako Ujihara, Keizo Nagasaki, Kazuhiro Hanazaki, Satoru Seo, Naoya Kitamura, Tetsuya Yamamoto, Shigenobu Matsuzaki","doi":"10.1093/femsle/fnae082","DOIUrl":"10.1093/femsle/fnae082","url":null,"abstract":"Bacteriophage (phage) KHP40 was previously isolated from the supernatant of a culture of Helicobacter pylori KMT83 cells. In this study, we analysed the infection characteristics of KHP40, phage release pattern from KMT83 cells, and state of KHP40 DNA in KMT83 cells. The findings revealed that KHP40 phage showed varied adsorption efficiencies for different strains, long latent periods, and small burst sizes. Additionally, KHP40 activity was maintained at pH 2.5-12. KHP40 phages were released during the vegetative growth phase of the KMT83 cells. PCR analysis demonstrated that KHP40 DNA was stably maintained in KMT83 clones. Next-generation sequencing analysis revealed the presence of two distinct types of circular double-stranded DNA in H. pylori KMT83 cells. One was an H. pylori-specific DNA consisting of 1 578 403 bp, and the other was a 26 412-bp sequence that represented the episomal form of phage KHP40 DNA. Furthermore, defective KHP40-lysogenic DNA was detected in the H. pylori-specific DNA, the deleted portion of which appeared to have been transferred to another location in the bacterial genome. These findings indicate that KHP40 DNA exists in both episomal and defectively lysogenized states in KMT83 cells, and active phages are produced from KHP40-episomal DNA.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142389224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pangenome analysis of the environmental source Salmonella enterica highlights a diverse accessory genome and a distinct serotype clustering. 对环境源肠炎沙门氏菌的庞基因组分析突出显示了多样化的附属基因组和独特的血清型聚类。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae090

José Roberto Aguirre-Sánchez, Cristóbal Chaidez, Nohelia Castro-Del Campo

{"title":"The pangenome analysis of the environmental source Salmonella enterica highlights a diverse accessory genome and a distinct serotype clustering.","authors":"José Roberto Aguirre-Sánchez, Cristóbal Chaidez, Nohelia Castro-Del Campo","doi":"10.1093/femsle/fnae090","DOIUrl":"10.1093/femsle/fnae090","url":null,"abstract":"Salmonella remains the leading cause of foodborne infections globally. Environmental reservoirs, particularly aquatic bodies, serve as conduits for the fecal-oral transmission of this pathogen. While the gastrointestinal tract is traditionally considered the primary habitat of Salmonella, mounting evidence suggests the bacterium's capacity for survival in external environments. The application of advanced technological platforms, such as next-generation sequencing, facilitates a comprehensive analysis of Salmonella's genomic features. This study aims to characterize the genomic composition of Salmonella isolates from river water, contributing to a potential paradigm shift and advancing public health protection. A total of 25 river water samples were collected and processed, followed by microbiological isolation of Salmonella strains, which were then sequenced. Genomic characterization revealed adaptive mechanisms, including gene duplication. Furthermore, an open pangenome, predisposed to incorporating foreign genetic material, was identified. Notably, antibiotic resistance genes were found to be part of the core genome, challenging previous reports that placed them in the accessory genome.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of adenylosuccinate synthase purA increases susceptibility to colistin in Escherichia coli. 敲除腺苷琥珀酸合成酶 purA 会增加大肠杆菌对可乐定的敏感性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2024-01-09 DOI: 10.1093/femsle/fnae007

Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

{"title":"Knockout of adenylosuccinate synthase purA increases susceptibility to colistin in Escherichia coli.","authors":"Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito","doi":"10.1093/femsle/fnae007","DOIUrl":"10.1093/femsle/fnae007","url":null,"abstract":"Colistin is a cationic cyclic antimicrobial peptide used as a last resort against multidrug-resistant gram-negative bacteria. To understand the factors involved in colistin susceptibility, we screened colistin-sensitive mutants from an E. coli gene-knockout library (Keio collection). The knockout of purA, whose product catalyzes the synthesis of adenylosuccinate from IMP in the de novo purine synthesis pathway, resulted in increased sensitivity to colistin. Adenylosuccinate is subsequently converted to AMP, which is phosphorylated to produce ADP, a substrate for ATP synthesis. The amount of ATP was lower in the purA-knockout mutant than that in the wild-type strain. ATP synthesis is coupled with proton transfer, and it contributes to the membrane potential. Using the membrane potential probe, 3,3'-diethyloxacarbocyanine iodide [DiOC2(3)], we found that the membrane was hyperpolarized in the purA-knockout mutant compared to that in the wild-type strain. Treatment with the proton uncoupler, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), abolished the hyperpolarization and colistin sensitivity in the mutant. The purA-knockout mutant exhibited increased sensitivity to aminoglycosides, kanamycin, and gentamicin; their uptake requires a membrane potential. Therefore, the knockout of purA, an adenylosuccinate synthase, decreases ATP synthesis concurrently with membrane hyperpolarization, resulting in increased sensitivity to colistin.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10876104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139671609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0