Fems Microbiology Letters最新文献_第8页

Identification and expression of a hydrophobic carboxylic acid reductase from Trichoderma virens. 绿木霉疏水性羧酸还原酶的鉴定与表达。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf021

Andrew B H Goh, Jonathan G Ling, Shazilah Kamaruddin, Abdul M A Murad, Farah D Abu Bakar

{"title":"Identification and expression of a hydrophobic carboxylic acid reductase from Trichoderma virens.","authors":"Andrew B H Goh, Jonathan G Ling, Shazilah Kamaruddin, Abdul M A Murad, Farah D Abu Bakar","doi":"10.1093/femsle/fnaf021","DOIUrl":"10.1093/femsle/fnaf021","url":null,"abstract":"Carboxylic acid reductases (CARs) have been garnering attention in applications for the sustainable synthesis of aldehydes. Despite numerous discoveries, not all characteristics of CAR enzymes have been extensively studied or understood. Herein, we report the discovery and expression of a new CAR enzyme (TvirCAR2) from the ascomycetous fungus, Trichoderma virens. Tvircar2 is one of the five putative CARs identified from analyses of the T. virens genome. In silico, analyses showed that TvirCAR2 has a high hydrophobicity index and that its corresponding gene is part of a biosynthetic gene cluster predicted to synthesize hybrid polyketide synthases-nonribosomal peptide synthetase secondary metabolites. TvirCAR2 was highly expressed as soluble and insoluble forms in an Escherichia coli expression host. The solubility of the purified TvirCAR2 necessitated the addition of glycerol in the purification and assay buffers. Substrate screening via molecular docking showed that benzoic acid was a suitable substrate candidate. The TvirCAR2 enzyme catalyzed the reduction of benzoic acid with a specific activity of around 1.4 µmol/h/mg. Homologs, which are predicted to exhibit similar hydrophobicity, are the CARs from Stachybotrys bisbyi (StbB), which is involved in the production of the meroterpenoid, ilicicolin B, and Trichoderma reesei (TrCAR), which is part of a similar but still uncharacterized biosynthetic gene cluster.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing methane production from coal under the application of an electric field system by enriching microorganisms on electrode. 在电场系统的作用下，通过在电极上富集微生物来提高煤的甲烷产量。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf053

Jiajia Shi, Jiayan Zhang, Hongguang Guo, Xiaogang Mu, Longzhen Gao, Zaixing Huang, Michael Urynowicz

{"title":"Increasing methane production from coal under the application of an electric field system by enriching microorganisms on electrode.","authors":"Jiajia Shi, Jiayan Zhang, Hongguang Guo, Xiaogang Mu, Longzhen Gao, Zaixing Huang, Michael Urynowicz","doi":"10.1093/femsle/fnaf053","DOIUrl":"10.1093/femsle/fnaf053","url":null,"abstract":"The functional microbial community, especially the electrode microorganisms, are crucial for the increment of biomethane production from coal by the application of an electric field. In this paper, the effect of electrode microorganisms on methane production from coal was investigated. They were enriched using the electrically domesticated flora (EF) and the primitive flora (PF) under 1.2 V, respectively. The methane production in EF and PF increased significantly by up to 22.75% and 40.02% compared with the nonenriched group (NF), suggesting that the enrichment of electrode microorganisms is an effective approach to enhance methane production from coal. When methane production ended, both the microbial community on electrode and in culture solution changed significantly. The proportions of such electroactive bacteria as Desulfovibrio and Thermincola on electrode in PF were 222.14% and 15466.67%, respectively higher than those in NF, while the proportion of fermentative bacterium Ramlibacter in EF increased by 979.42%. The bacterial richness and diversity in the culture solution decreased distinctly in EF and PF, revealing that the functional bacteria were transferred from culture solution to electrode in the effect of electric field. Fourier transform infrared spectrometer results verified that the degradation of 4,5-adjacent aromatic H deformation and C-O in coal were mainly promoted. And the enrichment of electrode microorganisms led to the accumulation of ketones and acids and the consumption of esters as revealed by gas chromatography-mass spectrometry. These findings indicated that methane production was enhanced by enriching electroactive and fermentative microbes on electrode which further improved coal biodegradation. It also provided a new way to further increase methane production from coal and promoted the application of bioelectrochemical system in microbially enhanced coalbed methane.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T. regifaucium Amycolatopsis DSM 45072T编码decaplanin和kigamicin的NRPS和II型PKS生物合成基因簇的鉴定

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf049

Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur

{"title":"Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T.","authors":"Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur","doi":"10.1093/femsle/fnaf049","DOIUrl":"10.1093/femsle/fnaf049","url":null,"abstract":"Amycolatopsis regifaucium, a Gram-positive actinomycete, is a prolific source of biologically active compounds, including polyphenol antibiotics like kigamicins. This study presents the draft genome of A. regifaucium DSM 45072T (= GY080T), which spans 8.28 Mbp and is assembled into 62 contigs, with annotation revealing 312 subsystems, 7966 coding genes, and 52 RNAs, with a GC content of 68.5 mol%. We found a significant genomic diversity within the genus, revealing variations in core and accessory genomic elements across species. Multiple biosynthetic gene clusters have been identified, including a previously unidentified glycopeptide antibiotic gene cluster and a type II polyketide synthase gene cluster, highlighting the organism's metabolic versatility and potential for the biosynthesis of novel natural products. Our analysis confirmed the production of decaplanin, an antibiotic previously attributed to Amycolatopsis decaplanina DSM 44594T. Correspondingly, the gene cluster responsible for decaplanin biosynthesis is identified in A. regifaucium DSM 45072T and A. decaplanina DSM 44594T. Additionally, a putative type II PKS gene cluster is predicted within the glycopeptide antibiotic-producing clade (Cluster A) of the genus Amycolatopsis. Genomics insights from A. regifaucium DSM 45072T represent it as a promising genetic resource with significant implications for biotechnological and pharmaceutical innovation, particularly in discovering and developing novel antimicrobial agents.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annonacin accumulation in leaves of Annona muricata L. induced by mycorrhizal colonization. 菌根定殖诱导的番荔枝叶片中番荔枝酸积累。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf085

Angela Michelle González-López, Evangelina Esmeralda Quiñones-Aguilar, Cecilia Guizar-González, Gabriel Rincón-Enríquez

{"title":"Annonacin accumulation in leaves of Annona muricata L. induced by mycorrhizal colonization.","authors":"Angela Michelle González-López, Evangelina Esmeralda Quiñones-Aguilar, Cecilia Guizar-González, Gabriel Rincón-Enríquez","doi":"10.1093/femsle/fnaf085","DOIUrl":"10.1093/femsle/fnaf085","url":null,"abstract":"Annona muricata L. is a tropical tree known for its secondary metabolites, particularly acetogenins, which have cytotoxic and antitumor properties. Research has shown that arbuscular mycorrhizal fungi (AMF) symbiosis and drought stress can increase the production of terpenoids, alkaloids, and phenolic compounds in plants. Our objective was to assess whether AMF symbiosis (both species and consortia) and two irrigation regimens enhanced foliar annonacin concentration, the primary acetogenin in A. muricata leaves. Two irrigation levels were used: normal irrigation (NI) and low irrigation (LI). Trees were inoculated with two AMF consortia: Cerro del Metate (CM) and Agua Dulce (AD); and two AMF species: Rhizophagus intraradices and Funneliformis mosseae (FM). Results showed that annonacin concentration was 83% lower in leaves under LI compared to NI. However, AMF symbiosis increased annonacin concentration, especially under LI conditions. Dry leaf weight was higher in mycorrhizal plants under the LI level than in controls. No growth promotion due to AMF symbiosis was observed under NI. In conclusion, AMF symbiosis promotes foliar annonacin concentration in A. muricata leaves in the two irrigation levels. FM treatment promotes higher annonacin concentration in the NI condition, while AD, CM, and FM treatments promotes annonacin concentration in the LI condition.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annual grass invasion is associated with differences in the community structure and abundance of biocrusts and arbuscular mycorrhizal fungi. 禾草年入侵与群落结构、生物结皮和丛枝菌根真菌丰度的差异有关。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf071

Rachel Z Berner, Geoffrey Zahn, Alexis A Spencer, V Bala Chaudhary, Tanya E Cheeke

{"title":"Annual grass invasion is associated with differences in the community structure and abundance of biocrusts and arbuscular mycorrhizal fungi.","authors":"Rachel Z Berner, Geoffrey Zahn, Alexis A Spencer, V Bala Chaudhary, Tanya E Cheeke","doi":"10.1093/femsle/fnaf071","DOIUrl":"10.1093/femsle/fnaf071","url":null,"abstract":"Interactions between biocrusts and arbuscular mycorrhizal (AM) fungi may increase plant resilience to environmental stress in native grasslands. When non-native plants invade, they may have cascading effects on soil biota and their interactions, destabilizing grassland ecosystems. In a field survey we assessed the % cover and composition of biocrusts in plots varying in % cover of the invasive annual grass, Ventenanta dubia (Native, Transition, Invaded plots). We used field-collected soil as inocula in a glasshouse experiment to test whether the abundance of viable AM fungal propagules differs under biocrusts compared with bare soil, or across invasion levels. We used 18S rRNA gene sequencing on field-collected soil to test whether the structure of the AM fungal community differs under biocrusts versus bare soil across invasion levels. Invasion by V. dubia was associated with lower biocrust cover, distinct biocrust community structure, lower abundance of viable AM fungal propagules below biocrusts, and distinct AM fungal community structure with invasion. Our results support broader United Nations Sustainable Development Goals focused on the protection and restoration of terrestrial ecosystems. A better understanding of the interactions among plants, biocrusts, and AM fungi is important for mitigating the impacts of invasive species and informing grassland restorations.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life on the rocks: polyphasic evaluation of three epilithic cyanobacterial strains isolated from a single rock, with the description of Nostoc sikkimense sp. nov., from the northeastern region of India. 岩石上的生命：对从单一岩石中分离出的三种附石蓝藻菌株进行多相评价，并描述了来自印度东北部地区的 Nostoc sikkimense sp.

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf037

Sagarika Pal, Harsh Pant, Naresh Kumar, Priya, Shubham Singh, Nainshi Gupta, Prashant Singh

{"title":"Life on the rocks: polyphasic evaluation of three epilithic cyanobacterial strains isolated from a single rock, with the description of Nostoc sikkimense sp. nov., from the northeastern region of India.","authors":"Sagarika Pal, Harsh Pant, Naresh Kumar, Priya, Shubham Singh, Nainshi Gupta, Prashant Singh","doi":"10.1093/femsle/fnaf037","DOIUrl":"10.1093/femsle/fnaf037","url":null,"abstract":"Three epilithic cyanobacterial strains were isolated from the scrapings of a single rock surface from the Reshi River in Sikkim, India. At the time of sampling, the rock surface did not show any visible cyanobacterial growth; however, the surface of the rock was glistering. Subsequent morphological analysis indicated that two out of three strains exhibited typical Nostoc-like morphology and the third strain had cell division in multiple planes showing typical morphology of a member of the family Hapalosiphonaceae. Further, 16S rRNA gene phylogeny indicated the strains to be members of the genera Nostoc, Desmonostoc, and Westiellopsis. For species-level demarcation, additionally, 16S-23S ITS (Internal Transcribed Spacer) region analysis was performed, which indicated that the strain RESHI-1B-PS was a novel cyanobacterial lineage of the genus Nostoc, while the strains RESHI-1A-PS and RESHI-1C-PS were representatives of Desmonostoc sp. and Westiellopsis prolifica, respectively. Thus, in the current investigation, we have described an undocumented species of the cyanobacteria, which we named Nostoc sikkimense in accordance with the guidelines outlined in the International Code of Nomenclature for algae, fungi, and plants (ICN). The study also enumerates and illustrates different life cycle stages of N. sikkimense RESHI-1B-PS along with further expanding the geographic distribution of W. prolifica and its substantial ecological adaptability.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surprising effects of differential loss in genome evolution: the last-one-out. 基因组进化中差异损失的惊人影响：最后一个出局。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf051

Nico Bremer, William F Martin, Mike Steel

引用次数: 0

The genome-wide de novo mutations and icaA gene expression levels in Staphylococcus aureus under long-term subinhibitory and semisubinhibitory nisin exposure. 长期亚抑制性和半抑制性nisin暴露下金黄色葡萄球菌全基因组新生突变和icaA基因表达水平

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf059

Hüseyin Özgür Özdemirel, Sibel Kucukyildirim

{"title":"The genome-wide de novo mutations and icaA gene expression levels in Staphylococcus aureus under long-term subinhibitory and semisubinhibitory nisin exposure.","authors":"Hüseyin Özgür Özdemirel, Sibel Kucukyildirim","doi":"10.1093/femsle/fnaf059","DOIUrl":"10.1093/femsle/fnaf059","url":null,"abstract":"The emergence and rapid spread of multidrug-resistant pathogens have caused a need for alternative antimicrobials, and bacteriocins are considered promising alternatives due to their lower risk of resistance development. Regarding this, we aimed to investigate the long-term subinhibitory and semisubinhibitory concentrations of a commonly used bacteriocin (nisin) in Staphylococcus aureus using an experimental evolution approach followed by genome sequencing. We then performed RT-qPCR to examine changes in the expression level of the biofilm-related icaA gene in evolved lines. We found that while nisin treatment did not significantly elevate the base-substitution rates, there was a significant decrease in the insertion/deletion rate in the lines exposed to the subinhibitory concentration of nisin. We also revealed an increase in nonsynonymous mutations in specific genes (e.g. sarS and cap8) associated with resistance and virulence mechanisms. Importantly, we observed a transition bias in the nisin-treated lines for the first time, and it may be related to the resistance development to nisin. RT-qPCR analysis of the icaA gene showed a reduced expression levels in nisin-treated groups, although the results were not statistically significant. These findings show the potential outcomes of nisin exposure in S. aureus and emphasize the need for careful consideration of bacteriocins in clinical practice.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12199728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory activity of meso-dimercaptosuccinic acid against IMP metallo-β-lactamase variants in Pseudomonas aeruginosa. 中二巯基琥珀酸对铜绿假单胞菌IMP金属β-内酰胺酶变异的抑制作用。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf052

Jacqueline Findlay, Hector Moreno, Gilbert Greub, Christophe Le Terrier, Maxime Bouvier, Patrice Nordmann

{"title":"Inhibitory activity of meso-dimercaptosuccinic acid against IMP metallo-β-lactamase variants in Pseudomonas aeruginosa.","authors":"Jacqueline Findlay, Hector Moreno, Gilbert Greub, Christophe Le Terrier, Maxime Bouvier, Patrice Nordmann","doi":"10.1093/femsle/fnaf052","DOIUrl":"10.1093/femsle/fnaf052","url":null,"abstract":"The treatment of infections caused by MBL-producing Pseudomonas aeruginosa, including IMP-producers, can be challenging since MBLs confer resistance to most clinically available ß-lactams, leaving few viable options. Thiol-containing compounds, such as meso-dimercaptosuccinic acid (DMSA), have been suggested as inhibitors of MBLs, and particularly have been proposed to exert effective activity against IMP-1 enzymes. In this context, we sought to determine the activity of DMSA, in combination with carbapenems or cephalosporins, against diverse MBL and carbapenemase variants, particularly focussing on IMP variants. In recombinant strains producing either IMP-1, IMP-10, or IMP-13, both with and without a functional OprD, synergy was observed with ceftazidime (CAZ), cefepime (FEP), and meropenem. Negligible changes were observed for other MBLs and no effect was observed in stains producing KPC-2. Testing of the CAZ/DMSA and FEP/DMSA combinations against clinical IMP-producing P. aeruginosa isolates resulted in fractional inhibitory concentration indexes indicating synergy. Enzymatic assays showed a significantly increased inhibitory activity of DMSA against the IMP variants, compared to the other enzymes tested. These findings highlight a possible role of DMSA or DMSA-like compounds to be developed for the treatment of infections caused by IMP-producing P. aeruginosa.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Golgi_traff phylogeny reveals ancient eukaryotic genes with recent surprises: replication and diversification of HID1 domain-containing protein unique to Schizosaccharomyces. Golgi_traff系统发育揭示了古老的真核基因，最近的惊喜：分裂糖菌特有的HID1结构域蛋白的复制和多样化。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf088

Mark A Hooks, Abdulrahman Alasmari, Mohammed Alshehri, Lysiane Brocard, Katarzyna B Hooks, Mathilde Julien, Ramsay J McFarlane

{"title":"Golgi_traff phylogeny reveals ancient eukaryotic genes with recent surprises: replication and diversification of HID1 domain-containing protein unique to Schizosaccharomyces.","authors":"Mark A Hooks, Abdulrahman Alasmari, Mohammed Alshehri, Lysiane Brocard, Katarzyna B Hooks, Mathilde Julien, Ramsay J McFarlane","doi":"10.1093/femsle/fnaf088","DOIUrl":"10.1093/femsle/fnaf088","url":null,"abstract":"Golgi_traff is a Pfam clan containing two members, Dymeclin (DYM) and HID1 domain-containing protein (HID). Interrogation of over 900 eukaryotic genomes with sequence models showed that both are ancient eukaryotic genes, which have exhibited different paths of gene loss, including from major taxonomic groups. For example, the Metazoa have both genes, whereas the Viridiplantae and Dikarya have lost HID and DYM, respectively. A unique replication event occurred within the genus Schizosaccharomyces in that all sequenced species possess three HID-encoding paralogs, whereas its nearest fungal relatives and other eukaryotes are almost exclusively monogenic. A phylogenetic analysis of yeasts revealed that the Golgi-resident paralog Human ortholog 3 (SPAC17A5.16) is more similar to the HID of other yeasts than to its paralogs. Transmission electron microscopy revealed that the SPAC17A5.16 mutant lacks a stacked Golgi apparatus (GA) form, suggesting a role in maintaining GA structure. Altered proliferation of the SPAC17A5.16 mutant in response to GA disrupting chemical agents indicated a perturbation of GA-related functions. Structural models suggest SPAC17A5.16 has a long, disordered N-terminal region that may facilitate anchoring to GA membranes. A modification to Schizosaccharomyces HID nomenclature is proposed to reflect their evolutionary and functional characteristics. The potential of the Golgi_traff clan to serve as a model for the diversification of protein function according to the concepts of sub/neofunctionalization is discussed.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12416282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144948225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0