Fems Microbiology Letters最新文献_第7页

Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity. 位于VIM-2活性位点附近的保守辅助残基影响其金属β-内酰胺酶活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf007

Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh

{"title":"Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity.","authors":"Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh","doi":"10.1093/femsle/fnaf007","DOIUrl":"10.1093/femsle/fnaf007","url":null,"abstract":"Verona-integron-metallo-β-lactamase (VIM-2) is one of the most widespread class B β-lactamase responsible for β-lactam resistance. Although active-site residues help in metal binding, the residues nearing the active-site possess functional importance. Here, to decipher the role of such residues in the activity and stability of VIM-2, the residues E146, D182, N210, S207, and D213 were selected through in-silico analyses and substituted with alanine using site-directed mutagenesis. The effects of substitution mutations were assessed by comparing the changes in β-lactam susceptibility pattern of Escherichia coli host cell expressing VIM-2 and its mutated proteins. VIM-2_N210A enhanced the susceptibility of the host by ∼4-8 folds against penicillins and cephalosporins, while the expression of VIM-2_D182A radically increased the susceptibility of host. However, expression of VIM-2_E146A reduced the susceptibility of host by 2-fold. Further, proteins were purified to homogeneity, and VIM_N210A and VIM_D182A displayed reduced thermal stability than VIM-2. Moreover, in vitro catalytic efficiencies of VIM-2_D182A were drastically reduced against all the β-lactams tested whereas the same were moderately reduced for VIM-2_N210A. Conversely, the catalytic efficiency was marginally altered for VIM_E146A. Overall, we infer that both N210A and D182A substitutions negatively affect the performance of VIM-2 by influencing substrate specificity and stability, respectively.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urine microbiome profiles in patients with different types of kidney stones. 不同类型肾结石患者尿液微生物组特征分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf054

Muhammed Manzoor, Mangesh Vasant Suryavanshi, Musliyarakath Mujeeburahiman, Ananthapadmanabha Bhagwath Arun, Aaron W Miller, Yogesh S Shouche, Punchappady Devasya Rekha

{"title":"Urine microbiome profiles in patients with different types of kidney stones.","authors":"Muhammed Manzoor, Mangesh Vasant Suryavanshi, Musliyarakath Mujeeburahiman, Ananthapadmanabha Bhagwath Arun, Aaron W Miller, Yogesh S Shouche, Punchappady Devasya Rekha","doi":"10.1093/femsle/fnaf054","DOIUrl":"10.1093/femsle/fnaf054","url":null,"abstract":"Kidney stone disease (KSD) is a multifactorial condition influenced by systemic and extrinsic factors, including diet, genetics, metabolic disorders, and microbial and environmental factors. Although preliminary evidence links the urinary microbiome (UMB) to stone formation, its role in different stone types remains unclear. We analysed UMB in patients with four distinct types of kidney stones (calcium oxalate, struvite, uric acid, and mixed composition). Urine samples from patients with KSD and controls were analysed using 16S rRNA gene sequencing. Patients with KSD exhibited distinct microbiota compositions, with increased abundances of Corynebacterium, Prevotella, and Staphylococcus than controls. The abundance of Dongia and Stenotrophomonas was higher in pure-stone formers than in mixed-stone formers (P < .05). Calcium oxalate stone formers had elevated levels of Pseudomonas and Dongia and reduced levels of Peptoniphilus than controls (P < .05). No significant differences in microbial diversity were observed between groups. Microbial composition correlated with blood and urine parameters, suggesting a potential influence on metabolic health and stone formation. These findings underscore the important role of UMB in KSD and provide valuable insights into its involvement in disease development and new opportunities for microbiome-based therapeutic strategies.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct microbial communities of drain flies (Clogmia albipunctata) across sites with differing human influence. 不同地点的排水蝇（Clogmia albipunctata）不同的微生物群落与不同的人类影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf078

Hyun Seo Park, Xavier Chavarria, Arwa Shatta, Dongjun Kang, Singeun Oh, Du-Yeol Choi, Jun Ho Choi, Myungjun Kim, Yoon Hee Cho, Myung-Hee Yi, Ju Yeong Kim

{"title":"Distinct microbial communities of drain flies (Clogmia albipunctata) across sites with differing human influence.","authors":"Hyun Seo Park, Xavier Chavarria, Arwa Shatta, Dongjun Kang, Singeun Oh, Du-Yeol Choi, Jun Ho Choi, Myungjun Kim, Yoon Hee Cho, Myung-Hee Yi, Ju Yeong Kim","doi":"10.1093/femsle/fnaf078","DOIUrl":"10.1093/femsle/fnaf078","url":null,"abstract":"Drain flies (Clogmia albipunctata) are insects that thrive in humid urban environments such as bathrooms drains and sewage systems. While their role in pathogen transmission has been suggested, little is known about their microbiome or ecology in non-clinical contexts. Using 16S rRNA gene metabarcoding, we characterized the bacterial communities of drain flies from three locations in South Korea, public bathrooms from a college in Seoul, a rural port in Ulleungdo island, and a highly frequented public park in Yeouido. In total, we obtained 221 families and 1 474 features. We found significant differences in microbiome composition and diversity as well as a small core microbiome shared among locations, with environmental bacteria such as Pseudomonas and Ralstonia being the dominant taxa across samples. The majority of the detected amplicon sequence variants (ASV) were not shared among locations. These findings suggest drain fly transport a location-specific environmental bacteria. Notably, we also identified ASVs of potential clinical relevance, including Mycobacterium, Acinetobacter baumanii, Providencia, and Nocardia. This is the first metagenomic insight into the microbiome of this species and adds to a renewed interest in the role that non-hematophagous insects play in urban microbial ecology and the spread of microbes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144834669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and functional analysis of two types of quorum sensing gene pairs, glaI1/glaR1 and glaI2/glaR2, in Burkholderia gladioli. 两类群体感应基因glaI1/ gl1和glaI2/ gl2在剑兰伯克霍尔德菌中的分布及功能分析

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae117

Kazumi Takita, Nobutaka Someya, Tomohiro Morohoshi

{"title":"Distribution and functional analysis of two types of quorum sensing gene pairs, glaI1/glaR1 and glaI2/glaR2, in Burkholderia gladioli.","authors":"Kazumi Takita, Nobutaka Someya, Tomohiro Morohoshi","doi":"10.1093/femsle/fnae117","DOIUrl":"10.1093/femsle/fnae117","url":null,"abstract":"Burkholderia gladioli produces a yellow-pigmented toxin called toxoflavin, and causes disease on a variety of plants. Previous studies have suggested that the pathogenicity of B. gladioli is regulated by an N-acyl-l-homoserine lactone (AHL)-mediated quorum sensing (QS) system. In this study, complete genome sequencing revealed that B. gladioli pv. gladioli MAFF 302385 possesses two types of AHL synthase and AHL receptor gene pairs: glaI1/glaR1 and glaI2/glaR2. Disruption of QS genes revealed that the glaI1/glaR1 QS system regulated swarming motility, biofilm formation, and colony formation via N-octanoyl-l-homoserine lactone. Although Escherichia coli harboring glaI2 produced N-(3-hydroxyoctanoyl)-l-homoserine lactone and N-(3-hydroxydecanoyl)-l-homoserine lactone, the expression of glaI2 was not confirmed in MAFF 302385 cells. We also found that toxoflavin production was regulated by the glaI1/glaR1 QS system in liquid medium, but not on agar medium. When pathogenicity tests were performed on gladiolus leaves, the wild-type and QS mutants showed a similar level of disease. Our results demonstrated that only the glaI1/glaR1-mediated QS system is active in MAFF 302385, but major virulence factors, especially toxoflavin, are not completely dependent on the QS system.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium antagonism potential and metabolomics analysis of endophytic bacteria isolated from Crotalaria retusa L., a traditional medicinal plant in Côte d'Ivoire. Côte科特迪瓦传统药用植物Crotalaria retusa L.内生细菌拮抗镰刀菌潜能及代谢组学分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf056

Evrad Sausthène Seka Ahoty, Romain Kouakou Fossou, Florent Magot, Anicet Théodore Ediman Ebou, Claude Ghislaine Zaka Kouadjo-Zézé, Baptiste Marchesseau, Rosella Spina, Jérémy Grosjean, Dominique Laurain-Mattar, Sophie Slezack, Adolphe Zézé

{"title":"Fusarium antagonism potential and metabolomics analysis of endophytic bacteria isolated from Crotalaria retusa L., a traditional medicinal plant in Côte d'Ivoire.","authors":"Evrad Sausthène Seka Ahoty, Romain Kouakou Fossou, Florent Magot, Anicet Théodore Ediman Ebou, Claude Ghislaine Zaka Kouadjo-Zézé, Baptiste Marchesseau, Rosella Spina, Jérémy Grosjean, Dominique Laurain-Mattar, Sophie Slezack, Adolphe Zézé","doi":"10.1093/femsle/fnaf056","DOIUrl":"10.1093/femsle/fnaf056","url":null,"abstract":"Sixty-four endophytic bacteria were isolated from roots, stems, and leaves of Crotalaria retusa L., a medicinal plant well-known for its antimicrobial properties in Côte d'Ivoire. Taxonomic characterization revealed that these bacteria were mainly dominated by the genera Pseudomonas, Rhizobium, Bacillus, and Inquilinus. The antagonistic activities of the endophytic bacteria against two phytopathogenic fungi affiliated with the genus Fusarium were tested using in vitro coculture. Isolates belonging to the genus Inquilinus showed the highest inhibitory activities against Fusarium oxysporum f. sp. cubense, ranging from 40% to 57%, while the highest inhibitory activities against Fusarium graminearum were obtained with Bacillus isolates (∼66%). Finally, a metabolomic study of the leaves, stems, and seeds of the plant and of the endophytes presenting antifungal activity was carried out using LC-MS/MS analysis of the methanolic extracts of all active endophytic isolates. The identified metabolites of interest from the endophytes were mainly peptides, lipids, and steroids. Two pyrrolizidine alkaloids, monocrotaline and senecionine, were detected in the plant organs but not in the endophytic bacterial extracts. These results highlighted the potential of C. retusa L. plant and its endophytic microbiome as a source of bioactive molecules of interest and biocontrol agents against phytopathogenic Fusarium spp.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144265793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival of Salmonella during dry salting process of pork feet. 猪脚干腌过程中沙门氏菌的存活。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf034

Rafaela Busnello, Luisa Aneiros Gené, Mariane Ferreira Silva, Fabiola Ayres Cacciatore, Nathanyelle Soraya Martins de Aquino, Eduardo Cesar Tondo

{"title":"Survival of Salmonella during dry salting process of pork feet.","authors":"Rafaela Busnello, Luisa Aneiros Gené, Mariane Ferreira Silva, Fabiola Ayres Cacciatore, Nathanyelle Soraya Martins de Aquino, Eduardo Cesar Tondo","doi":"10.1093/femsle/fnaf034","DOIUrl":"10.1093/femsle/fnaf034","url":null,"abstract":"Pork feet (PF) contaminated with Salmonella are often discarded by the food industry because heat treatment is not economically viable for these products. Dry salting can be an alternative to heat treatment if it can significantly reduce Salmonella populations. The aim of this study was to assess the Salmonella's survival in PF during dry salting process. Firstly, Salmonella was quantified in contaminated PF from the food industry. Thereafter, PF samples were artificially contaminated with three levels of Salmonella (3, 6, and 8 log CFU/g) and stored at 10°C for 30 days. Salmonella survival was assessed by counts on xylose lysine deoxycholate agar, mannitol lysine crystal-violet brilliant-green agar, and by the 3M™ 2 Molecular Detection Assay (MDA). After 18 days of salting, the 3 log CFU/g inoculum was no longer detected by the cultural and detection methods. The 6 and 8 log CFU/g inoculums were not detected on the agar plates after 26 days of salting but were still positive by MDA. Considering that Salmonella counts in contaminated PF in the food industry were 1.56 log CFU/g and that populations of 3-8 log CFU/g of Salmonella were reduced to unculturable levels after dry salting, this process could contribute to the control of Salmonella in PF.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bringing microbiology to life: problem-based learning activities enhance student engagement and clinical reasoning in the early medical curriculum. 将微生物学带入生活：基于问题的学习活动在早期医学课程中提高学生的参与度和临床推理能力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf075

Mônica Larucci Vieira

{"title":"Bringing microbiology to life: problem-based learning activities enhance student engagement and clinical reasoning in the early medical curriculum.","authors":"Mônica Larucci Vieira","doi":"10.1093/femsle/fnaf075","DOIUrl":"10.1093/femsle/fnaf075","url":null,"abstract":"Microbiology is a critical subject in medical education, but is often considered abstract, content-heavy, and disconnected from clinical practice by students. Active learning strategies, such as problem-based learning (PBL), can overcome these challenges by promoting critical thinking, contextualization of the concepts, and student participation. This study presents an application of PBL-inspired activities in large-group settings during the early years of the medical curriculum, an uncommon context for active methodologies. The students participated in two case-based discussion sessions inspired by PBL principles conducted in two steps: formulation of hypotheses based on prior knowledge and group discussion after independent research. At the end of the semester, students completed an anonymous questionnaire. The majority of the responders considered the activity intellectually adequate, integrating theoretical knowledge into practice and improving content retention. Students valued the clinical reasoning, interdisciplinary approach, autonomy, and motivation; suggestions for improvement were also provided. The implementation of active learning methods in medical microbiology enhanced involvement, promoted the contextualization of theoretical content, and supported meaningful learning in the participants' perception. This approach demonstrates the feasibility and perceived value of PBL in early medical education, even in resource-limited environments. It offers a model for educators aiming to enhance learning outcomes and student engagement with fundamental sciences, such as microbiology.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overlap and differences between the inward and outward electron transfer pathways in Shewanella oneidensis. 舍瓦氏菌向内和向外电子传递途径的重叠和差异。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf094

Kathryne C Ford, Shaylynn D Miller, Nicholas M Tefft, Michaela A TerAvest

{"title":"Overlap and differences between the inward and outward electron transfer pathways in Shewanella oneidensis.","authors":"Kathryne C Ford, Shaylynn D Miller, Nicholas M Tefft, Michaela A TerAvest","doi":"10.1093/femsle/fnaf094","DOIUrl":"10.1093/femsle/fnaf094","url":null,"abstract":"The extracellular electron transport chain of Shewanella oneidensis MR-1 has been well characterized in the context of current generation on an anode. However, work to understand electron uptake from the cathode is less mature and major questions remain regarding the pathway and purpose of electron uptake. To employ this organism as a biocatalyst for microbial electrosynthesis, we must have a clear picture of the path of electrons into the cell to mitigate off-target reactions and find opportunities for pathway improvement. In this work, we confirm that the outer membrane electron conduit MtrCAB is essential for electron uptake, while the inner membrane cytochrome CymA is important but can be partially compensated for by other proteins. Additionally, we show that endogenous flavins are important for electron uptake and their absence cannot be complemented by exogenous flavins. Finally, hydrogenases are not directly involved in electron transfer but may play a role in cell survival during stationary phase on the cathode. Overall, the inward electron transfer pathway largely overlaps with the outward electron transfer pathway although we find differences in the role of flavins, particularly exogenously added riboflavin.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic shifts induced by pH variation in Yarrowia lipolytica biofilm. pH值变化引起的多脂耶氏菌生物膜代谢变化。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf101

Akarawit Jenjitwanich, Hans Marx, Michael Sauer

{"title":"Metabolic shifts induced by pH variation in Yarrowia lipolytica biofilm.","authors":"Akarawit Jenjitwanich, Hans Marx, Michael Sauer","doi":"10.1093/femsle/fnaf101","DOIUrl":"10.1093/femsle/fnaf101","url":null,"abstract":"The yeast Yarrowia lipolytica adapts its metabolite production based on cultivation conditions, with the pH value playing a critical role. At pH 3, most Y. lipolytica strains produce polyols, while at pH 5, they accumulate predominantly organic acids. Yarrowia lipolytica has demonstrated the ability to transition from a planktonic, free-floating state to an immobilized state as a biofilm. This study aims to clarify the effects of pH level and carbon sources on the physiological state of Y. lipolytica when grown in a biofilm state. These pH variations were applied to the same biofilm culture to assess the capacity of given Y. lipolytica cells to undergo metabolic shifts and recovery under changing environmental conditions. Interestingly, a pH shift from 3 to 5 leads-as expected-to a metabolic shift from polyols to citric acid. However, the shift back to pH 3 does not revert to polyols as major products. This study not only revealed an unexpected production pattern but also provided benefits for the industrial process in general. Understanding biofilm cultivation methods supports continuous bioprocesses using the immobilized nature of biofilm. pH-alternating experiments reveal how environmental condition fluctuations affect biofilm culture physiology.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of glycolysis decreases sugar-induced cell death in Saccharomyces cerevisiae. 抑制糖酵解可降低糖诱导的酿酒酵母细胞死亡。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf026

Airat Valiakhmetov

{"title":"Suppression of glycolysis decreases sugar-induced cell death in Saccharomyces cerevisiae.","authors":"Airat Valiakhmetov","doi":"10.1093/femsle/fnaf026","DOIUrl":"10.1093/femsle/fnaf026","url":null,"abstract":"Although 30 years have passed since the description of sugar-induced cell death (SICD), the specific molecular mechanism that triggers this process remains unclear. This paper attempts to shed light on the relationship between SICD and glucose catabolism. In yeast cells, glucose is involved not only in energy-producing processes but also in the synthesis of reserve hydrocarbons. It is known that disruption of trehalose synthesis leads to significant changes in the physiology of Saccharomyces cerevisiae. The present study shows that deletion of the TPS1 gene resulted in a 44% suppression of SICD and a 75% reduction in the number of cells with excess ROS (reactive oxygen species). The suppression was comparable to the suppression of SICD (38%) and ROS (71%) with deletion of the HXK2 gene. Since HXK2 is the first enzyme in the glycolytic pathway, the effect of two other key glycolytic enzymes on SICD was tested. Deletion of the TDH3 gene (glyceraldehyde-3-phosphate dehydrogenase) resulted in a 39% suppression of SICD and ROS by 48%. Inhibition of Tdh3p with 1 mM iodoacetamide also suppressed SICD by 67% and ROS by 58%. Deletion of the PFK1 (phosphofructokinase 1) gene resulted in a complete block of SICD (97%) but unexpectedly resulted in a significant increase in the number of cells with excess ROS. The results obtained suggest that such a phenomenon as SICD is the result of an imbalance in the cellular pathways of glucose catabolism.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0