Fems Microbiology Letters最新文献_第7页

Environmental and host plant effects on taxonomic and phylogenetic diversity of root fungal endophytes. 环境和寄主植物对根真菌内生菌分类和系统发育多样性的影响。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf030

Emily C Farrer, Nelle K Kulick, Christina Birnbaum, Susannah Halbrook, Caitlin R Bumby, Claire Willis

{"title":"Environmental and host plant effects on taxonomic and phylogenetic diversity of root fungal endophytes.","authors":"Emily C Farrer, Nelle K Kulick, Christina Birnbaum, Susannah Halbrook, Caitlin R Bumby, Claire Willis","doi":"10.1093/femsle/fnaf030","DOIUrl":"10.1093/femsle/fnaf030","url":null,"abstract":"Nearly all plants are colonized by fungal endophytes, and a growing body of work shows that both environment and host species shape plant-associated fungal communities. However, few studies place their work in a phylogenetic context to understand endophyte community assembly through an evolutionary lens. Here, we investigated environmental and host effects on root endophyte assemblages in coastal Louisiana marshes. We isolated and sequenced culturable fungal endophytes from roots of three to four dominant plant species from each of three sites of varying salinity. We assessed taxonomic diversity and composition as well as phylogenetic diversity (mean phylogenetic distance, MPD) and phylogenetic composition (based on MPD). When we analyzed plant hosts present across the entire gradient, we found that the effect of the environment on phylogenetic diversity (as measured by MPD) was host dependent and suggested phylogenetic clustering in some circumstances. We found that both environment and host plant affected taxonomic composition of fungal endophytes, but only host plant affected phylogenetic composition, suggesting different host plants selected for fungal taxa drawn from distinct phylogenetic clades, whereas environmental assemblages were drawn from similar clades. Our study demonstrates that including phylogenetic, as well as taxonomic, community metrics can provide a deeper understanding of community assembly in endophytes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A two-component system MCNtrB/MCNtrC related to nitrogen metabolism in Micromonospora carbonacea. 碳小单孢子菌氮代谢相关的双组分体系MCNtrB/MCNtrC

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf016

Weiwei Xiang, Yuxin Long, Xingxing Zheng, Haiyan Ni, Long Zou, Yunhong Huang, Zhong-Er Long

{"title":"A two-component system MCNtrB/MCNtrC related to nitrogen metabolism in Micromonospora carbonacea.","authors":"Weiwei Xiang, Yuxin Long, Xingxing Zheng, Haiyan Ni, Long Zou, Yunhong Huang, Zhong-Er Long","doi":"10.1093/femsle/fnaf016","DOIUrl":"10.1093/femsle/fnaf016","url":null,"abstract":"Micromonospora carbonacea JXNU-1, a strain of an actinomycete with broad-spectrum antimicrobial activity, isolated from soil samples from the farmland in the area of Yaohu Lake in Nanchang, China, was taken as the object of study in this paper. Bioinformatics analysis revealed that there was a pair of proteins MCNtrB/MCNtrC homologous to the two-component system NtrB/NtrC, which usually exists only in the Gram-negative bacteria and is closely related to the regulation of nitrogen metabolism, in the whole cell protein of M. carbonacea. MCNtrB and MCNtrC, obtained by expression in vitro, were subjected to autophosphorylation and phosphate group transfer experiments. The results showed that MCNtrB had histidine kinase activity with the active site of His115, and MCNtrC can accept the phosphate group from phosphorylated MCNtrB with the active site of Asp33. The yeast two-hybrid experiments showed that MCNtrB and MCNtrC were a pair of proteins with a strong interaction. Overexpression of MCNtrB and MCNtrC in M. carbonacea can affect the expression of key enzymes in cellular nitrogen metabolism, such as glutamine synthetase, glutamate synthase, and glutamate dehydrogenase. These results indicated that MCNtrB/MCNtrC is a two-component system related to nitrogen metabolism in M. carbonacea, which could provide an important experimental basis for revealing the regulatory mechanism of nitrogen metabolism in M. carbonacea.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioaugmentation to enhance degradation of acetochlor and pretilachlor in water and sediment under anaerobic conditions. 在厌氧条件下，生物增强对水和沉积物中乙草胺和苯甲草胺的降解。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae114

Ha Danh Duc, Nguyen Thi Oanh

{"title":"Bioaugmentation to enhance degradation of acetochlor and pretilachlor in water and sediment under anaerobic conditions.","authors":"Ha Danh Duc, Nguyen Thi Oanh","doi":"10.1093/femsle/fnae114","DOIUrl":"10.1093/femsle/fnae114","url":null,"abstract":"Chloroacetamide herbicides are widely used to control weeds globally. In this study, three acetochlor-degrading mixed cultures using nitrate, sulfate, and ferric iron as electron acceptors were isolated and determined for their degradation under anaerobic conditions. The degradation rates of all mixed pure cultures in a mineral medium were not much different at 1 µM, while the rates at 50 µM were in the order: mixed culture using nitrate > sulfate > ferric iron as electron acceptors, giving 6.70, 6.13, and 4.85 µM/day, respectively. During acetochlor degradation, 2-ethyl-6-methyl-N-(ethoxymethyl)acetanilide, N-2-ethylphenyl acetamide, and 2-ethylaniline were transiently produced. Interestingly, the mixture of all cultures synergistically degraded pretilachlor although no individual strains could degrade the compound. Toluene and 2-ethylaniline were metabolites of pretilachlor degradation by the acetochlor-degrading bacteria. However, pretilachlor inhibited the acetochlor degradation by acetochlor-degrading bacteria. The inoculation of both acetochlor- and pretilachlor-degrading bacteria resulted in 91.4 ± 2.1% of acetochlor and 82.4 ± 2.6% of pretilachlor being utilized for 7 days. Moreover, the introduction of these degrading bacteria accelerated the degradation of both substrates contaminating water and sediment collected from a pond. This study provides insights into anaerobic degradation by pure cultures using different electron acceptors.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new mesophilic member of the Chloroflexota phylum 'Ca. Сhloroploca septentrionalis' from the meromictic lake Bol'shie Khruslomeny separated from the White Sea. 从白海分离出来的分生湖Bol‘shie Khruslominy中发现的氯藻门’Ca. Сhloroploca septentrionalis'的一个新的嗜温成员。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae113

Vladimir M Gorlenko, Denis S Grouzdev, Olga N Lunina, Vasil A Gaisin, Aleksandr A Ashikhmin, Maria A Sinetova

{"title":"A new mesophilic member of the Chloroflexota phylum 'Ca. Сhloroploca septentrionalis' from the meromictic lake Bol'shie Khruslomeny separated from the White Sea.","authors":"Vladimir M Gorlenko, Denis S Grouzdev, Olga N Lunina, Vasil A Gaisin, Aleksandr A Ashikhmin, Maria A Sinetova","doi":"10.1093/femsle/fnae113","DOIUrl":"10.1093/femsle/fnae113","url":null,"abstract":"A new filamentous phototrophic bacterium Khr17 was isolated as an enrichment culture from the brackish polar lake Bol'shie Khruslomeny. The organism was a halotolerant, strictly anaerobic phototroph possessing photosystem II. Sulfide was required for phototrophic growth. The cells of bacterium Khr17 formed nonmotile, wavy trichomes surrounded by a sheath. The cells contained chlorosomes, gas vesicles, and storage granules. The antenna pigments of bacterium Khr17 were bacteriochlorophyll c and β- and γ-carotenes. The genome of Khr17 bacterium carries all the genes responsible for CO2 fixation via the 3-hydroxypropionate pathway. The genes encoding the proteins of the nitrogenase complex were not found. The DNA G + C content was 59.9%. The 16S rRNA gene sequence of isolate Khr17 exhibited 99.4% similarity to related species. The average nucleotide identity and digital DNA-DNA hybridization values for the isolate showed 91.9% and 46.9% similarity, respectively, to other 'Ca. Chloroploca' species. Based on its phenotypic and phylogenetic characteristics, classification of Khr17 as member of a new species, 'Ca. Chloroploca septentrionalis' sp. nov., was proposed. Members of the genus 'Ca. Chloroploca' have previously not been found in Arctic areas and in the plankton of meromictic lakes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential for microbial denitrification coupled with methanol oxidation found in abundant MAGs in Antarctic Peninsula sediments. 在南极半岛沉积物中发现丰富的MAGs中存在微生物反硝化和甲醇氧化的潜力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf050

Katie E Howland, Hannah J Nygaard, Andrew D Steen, Kenneth M Halanych, Andrew R Mahon, Deric R Learman

{"title":"Potential for microbial denitrification coupled with methanol oxidation found in abundant MAGs in Antarctic Peninsula sediments.","authors":"Katie E Howland, Hannah J Nygaard, Andrew D Steen, Kenneth M Halanych, Andrew R Mahon, Deric R Learman","doi":"10.1093/femsle/fnaf050","DOIUrl":"10.1093/femsle/fnaf050","url":null,"abstract":"Denitrification accounts for a substantial nitrogen loss from environmental systems, shifting microbial composition and impacting other biogeochemical cycles. In Antarctica, rising temperatures cause increased organic matter deposition in marine sediments, which can significantly alter microbially mediated denitrification. To examine the genetic potential of microorganisms driving N-cycling in these sediments, benthic sediment cores were collected at two sites in the Weddell Sea, Antarctica. DNA was extracted from multiple depths at each site, resulting in the reconstruction of 75 high-quality metagenome-assembled genomes (MAGs). Forty-seven of these MAGs contained reductases involved in denitrification. MAGs belonging to the genus Methyloceanibacter were the most abundant MAGs at both sites and all depths, except depth 3-6 cmbsf at one site, where they were not identified. The abundance of these Methyloceanibacter MAGs suggests the potential for nitrate-driven methanol oxidation at both sites. MAGs belonging to Beggiatoaceae and Sedimenticolaceae were found to have the genetic potential to produce intermediates in denitrification and the complete pathway for dissimilatory nitrate reduction to ammonia. MAGs within Acidimicrobiia and Dadabacteria had the potential to complete the final denitrification step. Based on MAGs, Antarctic peninsula sediment communities have the potential for complete denitrification and dissimilatory nitrate reduction to ammonia via a consortium.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptations in development and secondary metabolism of Aspergillus nidulans isolates from the Erbil province in Iraq. 伊拉克埃尔比勒省细粒曲霉分离株发育和次生代谢的适应性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf043

Shkar R K Rostam, Christoph Sasse, Khattab A M Shekhany, Anna M Köhler, Rebekka Harting, Gerhard H Braus

{"title":"Adaptations in development and secondary metabolism of Aspergillus nidulans isolates from the Erbil province in Iraq.","authors":"Shkar R K Rostam, Christoph Sasse, Khattab A M Shekhany, Anna M Köhler, Rebekka Harting, Gerhard H Braus","doi":"10.1093/femsle/fnaf043","DOIUrl":"10.1093/femsle/fnaf043","url":null,"abstract":"Aspergillus nidulans is a common soil fungus, distributed worldwide. Adaptations in development and secondary metabolism of so far uncharacterized isolates from the Erbil province of Iraq were compared to each other and the commonly in laboratory work used strain A4 from Europe was used as a reference. Erb strains showed an increase in conidia formation during asexual development at 37°C when compared to A4. In contrast, fruiting body formation was strongly decreased during sexual development at 30°C or 37°C. The temperature had a strong impact on the synthesis of secondary metabolites. The amount of arugosin A, epi-/shamixanthone, and emericellin is increased in A4 compared to the Erb1 isolate during the various tested cultivation conditions. In contrast, the Erb1 isolate produced under these conditions increased amounts of sterigmatocystin. Deviations to the A4 in asperthecin production depends on the growth conditions e.g. changes in the incubation temperature. Increased conidiation of the Erb1 strain correlated with higher levels of austinol and dehydroaustinol production. These two metabolites were also more abundant in the Erb1 isolate during sexual development. Our data suggest that A. nidulans isolates from the Erbil province have adapted developmental and secondary metabolism patterns to local temperature conditions.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice 耐多药金黄色葡萄球菌感染噬菌体的分离和特性鉴定及其在小鼠中的治疗用途

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-09-13 DOI: 10.1093/femsle/fnae072

Zhen Xiao, Hongyi Xu, Juan Wang, Xueyuan Hu, Xiumei Huang, Shiping Song, Qingqing Zhang, Yanxin Liu, Yaopeng Liu, Na Liu, Junhui Liu, Ge Zhao, Xiyue Zhang, Yuehua Li, Jianmei Zhao, Junwei Wang, Huanqi Liu, Lin Wang, Zhina Qu

{"title":"Isolation and characterization of a multidrug-resistant Staphylococcus aureus infecting phage and its therapeutic use in mice","authors":"Zhen Xiao, Hongyi Xu, Juan Wang, Xueyuan Hu, Xiumei Huang, Shiping Song, Qingqing Zhang, Yanxin Liu, Yaopeng Liu, Na Liu, Junhui Liu, Ge Zhao, Xiyue Zhang, Yuehua Li, Jianmei Zhao, Junwei Wang, Huanqi Liu, Lin Wang, Zhina Qu","doi":"10.1093/femsle/fnae072","DOIUrl":"https://doi.org/10.1093/femsle/fnae072","url":null,"abstract":"In recent years, the emergence of multidrug-resistant bacteria has limited the selection of drugs for treating bacterial infections, reduced clinical efficacy, and increased treatment costs and mortality. It is urgent to find alternative antibiotics. In order to explore a new method for controlling methicillin resistant Staphylococcus aureus (S. aureus) , this study isolated and purified a multi drug resistant S. aureus broad-spectrum phage JPL-50 from wastewater. JPL-50 belongs to the Siphoviridae family after morphological observation, biological characterization, and transmission electron microscopy (TEM) fragmentation spectrum analysis. It can cleave 84% of tested S. aureus (168/200) , in which 100% of tested mastitis-associated strains (48/48) and 72.04% of MRSA strains (67/93) were lysed. In addition, it has an optimal growth temperature of about 30°C, a high activity within a wide pH range (pH 3–10) , and an optimal multiplicity of infection of 0.01. The one-step growth curve shows a latent time of 20 minutes, an explosive time of 80 minutes. JPL-50 was 16, 927 bp in length and was encoded by double-stranded DNA, with no genes associated with bacterial resistance or virulence factors detected. In a therapeutic study, injection of the phage JPL-50 once and for 7 times in 7 days protected 40% and 60% of the mice from fatal S.aureus infection, respectively. More importantly, JPL-50-doxycycline combination could effectively inhibit host S.aureus in vitro and reduce the use of doxycycline within 8 hours. In conclusion, the bacteriophage JPL-50 has a wide lysis spectrum, high lysis rate, high tolerance to extreme environments, and moderate in vivo activity, providing ideas for developing multidrug-resistant S. aureus infections.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"65 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organization, Conservation, and Diversity of Biosynthetic Gene Clusters in Bacillus sp. BH32 and Its Closest Relatives in the Bacillus cereus Group 蜡样芽孢杆菌 BH32 及其近亲中生物合成基因簇的组织、保存和多样性

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-09-09 DOI: 10.1093/femsle/fnae071

Hadj Ahmed Belaouni, Amine Yekkour, Abdelghani Zitouni, Atika Meklat

引用次数: 0

Dynamic responses of Salmonella Typhimurium to re-exposure to sublethal ciprofloxacin 鼠伤寒沙门氏菌对再次接触亚致死环丙沙星的动态反应

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-06-27 DOI: 10.1093/femsle/fnae050

Jiseok Yi, Junhwan Kim, Juhee Ahn

{"title":"Dynamic responses of Salmonella Typhimurium to re-exposure to sublethal ciprofloxacin","authors":"Jiseok Yi, Junhwan Kim, Juhee Ahn","doi":"10.1093/femsle/fnae050","DOIUrl":"https://doi.org/10.1093/femsle/fnae050","url":null,"abstract":"This study was designed to evaluate the history-dependent behaviors of Salmonella Typhimurium re-exposed to sublethal levels of ciprofloxacin. The S. Typhimurium cells were pre-exposed to 0 (CON), 1/16 (LOW), 1/8 (MED), and 1/4 (HIGH) MICs of ciprofloxacin, followed by re-exposure to the same concentrations. The bacterial growth, post-antibiotic effect (PAE), relative fitness, and swimming motility of treatments were evaluated in the absence of ciprofloxacin. The lag phase duration (LPD) was estimate to assess bacterial recovery under ciprofloxacin exposure. A disk diffusion assay was used to determine the cross-resistance and collateral sensitivity of CON, LOW, MED, and HIGH treatments to ciprofloxacin (CIP), ceftriaxone (CEF), erythromycin (ERY), gentamicin (GEN), and polymyxin B (POL). The S. Typhimurium cells pre-exposed to ciprofloxacin were susceptible in antibiotic-free media, showing delayed growth. The highest PAE (&gt; 1 h) and bacterial fluctuation (CV = 5%) were observed at the High treatment compared to the CON. The HIGH treatment had the lowest relative fitness levels (0.87) and swimming motility (55 mm). The LPD was significantly decreased at the LOW treatment (1.8 h) when re-exposed to 1/16× MIC of ciprofloxacin. The LOW, MED, and HIGH treatments showed the cross-resistance to POL and the collateral sensitivity to CEF, ERY, and GEN. The pre-exposure to ciprofloxacin could induce phenotypic diversity, corresponding to the history-dependent behaviors. These results provide important insights for the dynamic nature of bacterial populations when re-exposed to sublethal concentrations of antibiotics.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"140 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141502758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of PetrifilmTM AC and Pour plate techniques used for the heterotrophic aerobic bacterial count in water 用于水中异养需氧细菌计数的 PetrifilmTM AC 与倒平板技术的比较

IF 2.1 4区生物学

Fems Microbiology Letters Pub Date : 2024-04-30 DOI: 10.1093/femsle/fnae029

Faith Mkhwanazi, Tshilidzi Mazibuko, Olivia Mosoma, Malefaso Rathebe, Mrudula Patel

{"title":"Comparison of PetrifilmTM AC and Pour plate techniques used for the heterotrophic aerobic bacterial count in water","authors":"Faith Mkhwanazi, Tshilidzi Mazibuko, Olivia Mosoma, Malefaso Rathebe, Mrudula Patel","doi":"10.1093/femsle/fnae029","DOIUrl":"https://doi.org/10.1093/femsle/fnae029","url":null,"abstract":"Heterotrophic bacteria (HPC) are commonly found in water samples. While these bacterial counts do not necessarily indicate a health hazard, high counts provide a good indication of the efficiency of water disinfection and integrity of distribution systems. The aim of this study was to compare the PetrifimTM AC method to the Pour Plate technique for the testing of HPC in water samples. Artificially contaminated (192 samples) and natural water samples (25) were processed using two methods. Both methods accurately detected high, medium and low counts of HPC, producing average Z scores between -2 and + 2. Paired-wise student t-test and correlation coefficient showed nonsignificant differences between the results of two methods. Acceptable repeatability and reproducibility was obtained using both the methods. Uncertainty of measurement for PetrifilmTM AC and Pour Plate method was found to be 2.9% and 5.4% respectively. PetrifilmTM AC proved to be robust at 33 °C and 37 °C. In conclusion, PetrifimTM AC, which is easy to process, read and less time consuming, proved to be comparable to the conventional Pour Plate method in establishing HPC in water. In addition, PetrifimTM AC requires less space for the processing and incubation, generate small volume of waste for disposal and requires no equipment, except for the incubator.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"56 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140841262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0