Fems Microbiology Letters最新文献_第6页

Functionally important components of the transcription elongation complex involved in Rho-dependent termination. 转录延伸复合体的重要功能组分参与rho依赖性终止。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae111

Ajay Khatri, Ranjan Sen

{"title":"Functionally important components of the transcription elongation complex involved in Rho-dependent termination.","authors":"Ajay Khatri, Ranjan Sen","doi":"10.1093/femsle/fnae111","DOIUrl":"10.1093/femsle/fnae111","url":null,"abstract":"Bacterial transcription terminator, Rho is an RNA (Ribonucleic Acid)-dependent ATPase that terminates transcription. Several structures of pretermination complexes of the Rho-transcription elongation complex (EC) revealed a static picture of components of the EC that come close to the nascent RNA-bound Rho, where many of the residues of EC reside ≤10 Å from the Rho residues. However, the in vitro-formed Rho-EC complexes do not reveal the in vivo Rho-EC dynamic interaction patterns during the termination process. Here we report synthetic defect analyses of various combinations of the mutations in RNAP β, β' and ω-subunits, NusA, NusG, and Rho proteins to delineate the functional network of this process. Several mutations in the β-flap and β'-Zn-finger and -Clamp helices domains of RNAP are synthetically defective in the presence of Rho mutants indicating functional involvement of these domains. Mutations in the NusA RNA-binding domains were synthetically defective with the Rho mutants suggesting its involvement. Our genetic analyses also revealed functional antagonisms between the ω-subunit of RNAP and the NusG-CTD (c-terminal domain) during termination. We concluded that the regions surrounding the RNA exit channel, the RNA-binding domains of NusA, the RNAP ω-subunit, and NusG-CTD constitute a functional network with Rho just before the onset of in vivo Rho-dependent termination.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of Lactobacillus species from gut microbiota of Aegiale hesperiaris (Lepidoptera: Hesperiidae) larvae. 猕猴桃鳞翅目：猕猴桃科幼虫肠道菌群中乳杆菌的分离与鉴定。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf015

Ericka Denice Herrera-Cardoso, Karen Alejandra Tapia-Cervantes, Jonathan Cepeda-Negrete, Santiago Gutiérrez-Vargas, Ma Fabiola León-Galván

{"title":"Isolation and identification of Lactobacillus species from gut microbiota of Aegiale hesperiaris (Lepidoptera: Hesperiidae) larvae.","authors":"Ericka Denice Herrera-Cardoso, Karen Alejandra Tapia-Cervantes, Jonathan Cepeda-Negrete, Santiago Gutiérrez-Vargas, Ma Fabiola León-Galván","doi":"10.1093/femsle/fnaf015","DOIUrl":"10.1093/femsle/fnaf015","url":null,"abstract":"Entomophagy, the practice of consuming insects, is a global tradition. In Mexico, one of the most notable and widely consumed insects is the larva of Aegiale hesperiaris. This insect feeds on the leaves of various Agave species with high polysaccharide content, suggesting their potential role as prebiotics for the intestinal microbiota, particularly lactic acid bacteria (LAB). LAB are recognized for their use as probiotics in foods due to their health-promoting capabilities. In this study, LAB from the intestinal microbiota of A. hesperiaris larvae were isolated and characterized, utilizing 16S rRNA gene identification. The analysis revealed three bacterial species from the Lactobacillaceae family, indicating a close symbiotic relationship with the insect. This suggests a significant impact on carbohydrate and protein metabolism, vitamin synthesis, and amino acid production, contributing to the high nutritional value of this edible insect. The study provides insights into the bacteria within the digestive tract of A. hesperiaris larvae and their role in enhancing the nutritional value of this edible insect. Additionally, it establishes a foundation for future research on the ecological roles and potential biotechnological benefits of these bacteria in the food industry and the development of therapies for various conditions and diseases.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycorrhizal inoculation success depends on soil health and crop productivity. 菌根接种成功与否取决于土壤健康和作物生产力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf031

Ido Rog, Marcel G A van der Heijden, Franz Bender, Raphaël Boussageon, Antonin Lambach, Klaus Schlaeppi, Natacha Bodenhausen, Stefanie Lutz

{"title":"Mycorrhizal inoculation success depends on soil health and crop productivity.","authors":"Ido Rog, Marcel G A van der Heijden, Franz Bender, Raphaël Boussageon, Antonin Lambach, Klaus Schlaeppi, Natacha Bodenhausen, Stefanie Lutz","doi":"10.1093/femsle/fnaf031","DOIUrl":"10.1093/femsle/fnaf031","url":null,"abstract":"As the human population grows, so does the demand for higher agricultural yields. As a result, agricultural intensification practices are increasing while soil health is often declining. Integrating the benefits of microorganisms into agricultural management systems can reduce the need for external resource inputs. One particular group of plant symbionts that can help plants to acquire additional nutrients and promote plant growth are arbuscular mycorrhizal fungi (AMF). The application of AMF in agricultural practice has been hampered by the variability in the success of mycorrhizal inoculation and the lack of consistency in different fields. Here, we tested whether it is possible to predict mycorrhizal inoculation success based on soil health and productivity. We hypothesized higher inoculation success on fields with poor soil health because in such fields, mycorrhiza can improve nutrient uptake and biotic resistance to pathogens. We calculated a soil health index by aggregating six biotic and abiotic variables from 54 maize fields and tested its correlation with the mycorrhizal growth response (MGR). The MGR was linked to soil health and significantly higher in less healthy soils and less productive fields. This implies that soil inoculation with AMF has most potential in fields with poor soil health and low productivity. Based on these findings, we propose a soil health framework that highlights the potential benefits of AMF field inoculation.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa. α-生育酚对金黄色葡萄球菌、奇异变形杆菌和铜绿假单胞菌的生物膜破坏活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf020

Pui Yee Leong, Wei Qi Tan, Wee Sim Choo

{"title":"Biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa.","authors":"Pui Yee Leong, Wei Qi Tan, Wee Sim Choo","doi":"10.1093/femsle/fnaf020","DOIUrl":"10.1093/femsle/fnaf020","url":null,"abstract":"Antibiotic resistance and the persistence of sessile cells within biofilms complicate the eradication of biofilm-related infections using conventional antibiotics. This highlights the necessity for alternate therapy methods. The objective of this study was to investigate the biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa on polystyrene. α-Tocopherol showed significant biofilm destruction activity on the pre-formed biofilms of S. aureus (45%-46%), Pr. mirabilis (42%-54%), and Ps. aeruginosa (28%). Resazurin assay showed that α-tocopherol disrupted all bacterial biofilms without interfering with their cell viability. Scanning electron microscope images showed lower bacterial cell count and less compacted cell aggregates on polystyrene surfaces after treatment with α-tocopherol. This study demonstrated the biofilm destruction activity of α-tocopherol against S. aureus, Pr. mirabilis, and Ps. aeruginosa. α-Tocopherol could potentially be used to decrease biofilm-associated infections of these bacteria.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota. 表达关切：从人类肠道微生物群中分离出的新细菌物种 Limosilactobacillus caccae sp.

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf014

引用次数: 0

Correction to: Molecular hydrogen from water radiolysis as an energy source for bacterial growth in a basin containing irradiating waste. 更正：在含有辐照废物的水池中，作为细菌生长能量来源的水辐射分解产生的氢分子。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf003

引用次数: 0

The adoption of a superhero bacteria: Escherichia coli and its adventures in microbiology education. 超级英雄细菌的采用：大肠杆菌及其在微生物学教育中的冒险。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf042

Isabelle Carolina Cotrim Gozzi, Bruna Gennari Rosa, Isadora Medeiros, Beatriz Dinat Labone Silva, Giovanna Matos Rodrigues, Fernanda Ayumi Nagay Yoshihara, Giovanna Tresso Custódio, José Ricardo Ferreira de Lucena, Lara Nardi Baroni, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira

{"title":"The adoption of a superhero bacteria: Escherichia coli and its adventures in microbiology education.","authors":"Isabelle Carolina Cotrim Gozzi, Bruna Gennari Rosa, Isadora Medeiros, Beatriz Dinat Labone Silva, Giovanna Matos Rodrigues, Fernanda Ayumi Nagay Yoshihara, Giovanna Tresso Custódio, José Ricardo Ferreira de Lucena, Lara Nardi Baroni, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira","doi":"10.1093/femsle/fnaf042","DOIUrl":"https://doi.org/10.1093/femsle/fnaf042","url":null,"abstract":"Here, we present a case study of undergraduate biomedical students engaging in the Adopt a Bacterium active methodology. The study was designed and authored by students, associating heroes, and antiheroes with Escherichia coli to highlight its dual role in human health. By integrating elements of geek culture, we developed multimedia educational content, including social media posts, interactive chalkboard presentation, gamified seminar, and a comic book. This creative approach enabled a deeper understanding of microbiological concepts, improving communication skills, and stimulating engagement beyond the classroom. Our experience highlights the potential of integrating storytelling and visual media into science education to enhance motivation, autonomy, and practical/theoretical skills, alongside teamwork and science-based communication. Furthermore, we recognized that scientific dissemination involves making science accessible, empowering us to learn and share knowledge with future generations, thereby fostering a ripple effect in scientific literacy. By transforming abstract microbiological concepts into accessible and relatable narratives, the Adopt a Bacterium demonstrates the power of active methodologies in education and public engagement.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using core genome and machine learning for serovar prediction in Salmonella enterica subspecies I strains. 利用核心基因组和机器学习预测肠沙门氏菌I亚种菌株的血清型。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf040

Xiang Li, Adelumola Oladeinde, Michael Rothrock, Tae Jung Chung, Walid Ghazi Al Hakeem

{"title":"Using core genome and machine learning for serovar prediction in Salmonella enterica subspecies I strains.","authors":"Xiang Li, Adelumola Oladeinde, Michael Rothrock, Tae Jung Chung, Walid Ghazi Al Hakeem","doi":"10.1093/femsle/fnaf040","DOIUrl":"https://doi.org/10.1093/femsle/fnaf040","url":null,"abstract":"This study presents a dual investigation of Salmonella enterica subspecies I, focusing on serovar prediction and core genome characteristics. We utilized two large genomic datasets (panX and NCBI Pathogen Detection) to test machine learning methods for predicting Salmonella serovars based on genomic differences. Among the four tested algorithms, the Random Forest model demonstrated higher performance, achieving 90.3% accuracy with the panX dataset and 95.3% with the NCBI dataset, particularly effective when trained on >50% of available data. When combined with hierarchical clustering validation, our approach achieved 100% prediction accuracy on the simulated data. Parallel analysis of panX core genome characteristics revealed that pathogenicity-related genes (including sseA, invA, mgtC, phoP, phoQ, and sitA) exhibited similar phylogenetic topologies distinct from the core genome phylogenetic tree, suggesting shared evolutionary histories. Notably, all identified core antibiotic resistance genes and virulence factors showed evidence of negative selection, indicating their essential role in bacterial survival. This study not only presents a promising machine learning-based alternative for Salmonella serovar classification, particularly valuable when analyzing newly identified serovars alongside known reference strains but also provides insights into the evolutionary dynamics of core virulence-associated genes, contributing to our understanding of Salmonella genomic architecture and pathogenicity.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and activity of AHL-lactonases in Bacillus spp. from various environments. 不同环境下芽孢杆菌ahl内酯酶的多样性和活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf038

Iva Rosić, Ivan Nikolić, Marina Anteljević, Ivana Marić, Tamara Ranković, Slaviša Stanković, Tanja Berić, Olja Medić

{"title":"Diversity and activity of AHL-lactonases in Bacillus spp. from various environments.","authors":"Iva Rosić, Ivan Nikolić, Marina Anteljević, Ivana Marić, Tamara Ranković, Slaviša Stanković, Tanja Berić, Olja Medić","doi":"10.1093/femsle/fnaf038","DOIUrl":"10.1093/femsle/fnaf038","url":null,"abstract":"Disrupting quorum sensing (QS) pathways in animal and plant pathogenic bacteria is an effective strategy to mitigate infections without promoting antibiotic and pesticide resistance. This approach inhibits the production of virulence factors, biofilm formation, and toxin production, reducing bacterial pathogenicity. In plant health protection, Bacillus spp. are extensively researched and utilized as biocontrol agents; however, the potential of their AHL-lactonase-producing ability, which plays a key role as a QS inhibitor of Gram-negative pathogens, remains largely unexplored. This study examined the activity and diversity of QQ enzymes from Bacillus spp. isolates obtained from various natural sources, confirming their presence in previously unreported environments associated with agricultural fields (straw and manure). Our findings show that AiiA lactonase is the most dominant and highly conserved AHL-lactonase among Bacillus isolates from bulk soil, manure, and straw. Despite its sequence conservation, we observed significant variation in AiiA lactonase activities toward the N-hexanoyl-DL-homoserine lactone (C6-HSL) substrate. Furthermore, in silico analysis suggested that the Bacillus sp. YtnP lactonase may have a lower affinity for C6-HSL compared to AiiA lactonase. Finally, this research presents a selection of Bacillus isolates with high AiiA lactonase activity for potential testing against plant pathogens.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro enzyme characterization and several inhibitors for monkeypox virus core protease I7L. 猴痘病毒核心蛋白酶I7L的体外酶学特性及几种抑制剂。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf008

Lin Wei, Yuqi Wu, Shuai Li, Jun Weng, Miaomiao Geng, Meng Mei, Zigong Wei

引用次数: 0