Fems Microbiology Letters最新文献_第6页

Slx5/Slx8 SUMO-targeted ubiquitin ligase deficiency shortens lifespan due to increased mutation accumulation in yeast. Slx5/Slx8 sumo靶向泛素连接酶缺乏症由于酵母突变积累增加而缩短寿命。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae109

Pınar B Thomas, Nur Kaluç, Irmak N Çavlı, Bilge G Tuna

{"title":"Slx5/Slx8 SUMO-targeted ubiquitin ligase deficiency shortens lifespan due to increased mutation accumulation in yeast.","authors":"Pınar B Thomas, Nur Kaluç, Irmak N Çavlı, Bilge G Tuna","doi":"10.1093/femsle/fnae109","DOIUrl":"10.1093/femsle/fnae109","url":null,"abstract":"Chronological lifespan (CLS) in budding yeast Saccharomyces cerevisiae, which is defined as the time nondividing cells in saturation remain viable, has been utilized as a model to study post-mitotic aging in mammalian cells. CLS is closely related to entry into and maintenance of a quiescent state. Many rearrangements that direct the quiescent state enhance the ability of cells to endure several types of stress. Small ubiquitin-like modifier (SUMO)-targeted ubiquitin ligases (STUbLs) play a critical role in mediating an adaptive response to various stresses. In this study, we investigated the effect of a STUbL, Slx5/Slx8, on CLS in budding yeast. We showed that both SLX5 and SLX8 deletions accelerate chronological aging, resulting in a decreased maximum and mean lifespan. slx5Δ cells were capable of entering or maintaining a quiescent state during aging. On the other hand, aging slx5Δ and slx8Δ cells had both increased spontaneous mutation accumulation. Our data together indicate that Slx5/Slx8 STUbL is required for normal rate of aging by preventing increased spontaneous mutation accumulation during aging.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and enzymatic profiling reveals aminopeptidase potential of Lactobacillus acidophilus ItalPN270. 遗传和酶分析揭示了嗜酸乳杆菌ItalPN270的氨基肽酶潜力。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf028

Cristian Mauricio Barreto Pinilla, Frank Guzman Escudero, Leila Maria Spadoti, Adriano Brandelli, Adriana Torres Silva E Alves

{"title":"Genetic and enzymatic profiling reveals aminopeptidase potential of Lactobacillus acidophilus ItalPN270.","authors":"Cristian Mauricio Barreto Pinilla, Frank Guzman Escudero, Leila Maria Spadoti, Adriano Brandelli, Adriana Torres Silva E Alves","doi":"10.1093/femsle/fnaf028","DOIUrl":"10.1093/femsle/fnaf028","url":null,"abstract":"Lactobacillus acidophilus strains are considered probiotics and have several industrial applications, including their use as non-starter cultures in fermented milk products. However, their biotechnological potential was partially explored. This work investigated the potential peptidase activity of Lactobacillus acidophilus ItalPN270, by mining their whole genome for genetically encoded peptidases and a comparative in vitro analysis of aminopeptidase activity and lytic behavior. The results showed that the assembled bacterial genome comprised one circular chromosome (1 964 524 bp) with 34.57% GC content, and 1906 protein-coding sequences (CDSs). Analysis of the genome sequence of ItalPN270 revealed the presence of 25 genes that encode peptidases with different specificities. The ItalPN270 presented higher values of aminopeptidase activity in vitro, regarding the six enzymatic substrates evaluated, showing values of total aminopeptidase activity 4-fold higher, as compared with an L. paracasei and L. helveticus strains, and notable high activity of pepA, pepL, and pepX. Moreover, the strain ItalPN270 showed an autolysis profile defined by 63.4% of lysis in the first 5 days with low variations after 40 days at 13°C. Thus, our results indicated that strain L. acidophilus ItalPN270 is a potential source of peptidases for different applications, including as adjunct bacteria for improving cheese ripening.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice husk- and lemongrass-derived eco-enzymes as potential food contact surface disinfectants against biofilm-forming foodborne pathogens. 稻壳和柠檬草衍生的生态酶作为潜在的食品接触面消毒剂对抗生物膜形成的食源性病原体。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae116

Vickneish Vimalanathan, Hanan Hasan, Vickineshwari Kunasegaran, Kausalyaa Sarawanan, Monisha Ilangovan, Pratheep Sandrasaigaran

{"title":"Rice husk- and lemongrass-derived eco-enzymes as potential food contact surface disinfectants against biofilm-forming foodborne pathogens.","authors":"Vickneish Vimalanathan, Hanan Hasan, Vickineshwari Kunasegaran, Kausalyaa Sarawanan, Monisha Ilangovan, Pratheep Sandrasaigaran","doi":"10.1093/femsle/fnae116","DOIUrl":"10.1093/femsle/fnae116","url":null,"abstract":"This study aims to evaluate the rice husk (EE-R)- and lemongrass (EE-L)-derived eco-enzymes (EE) as alternatives to chemical-based disinfectants. The EE-R's and EE-L's antimicrobial activity were tested against Pseudomonas aeruginosa, Salmonella Typhimurium, and Staphylococcus aureus using a broth microdilution method. The antibiofilm activities of EE were determined using crystal violet staining. Lastly, the minimal contact time of EE for effectively reducing biofilm-forming pathogens (<25 CFU/ml) was assessed on various food contact surfaces (wood, glass, plastic, stainless steel, and marble). The results show that EE-R at 25%-50% concentration significantly inhibited P. aeruginosa and S. aureus while reducing the initial biofilm formation by 61% and 58%, respectively. In contrast, EE-L inhibited S. Typhimurium at a concentration of 12.5%-50% and P. aeruginosa at 25%-50%, with a strong preformed biofilm inhibition noticed for S. Typhimurium (70%). For the minimal contact time, EE-R superiorly inhibited P. aeruginosa (60 s) and S. aureus (120 s) on all contact surfaces, while EE-L needed 120 s to reduce P. aeruginosa and S. Typhimurium. These outcomes were comparable to sodium hypochlorite (NaOCl, 2.5%). The study's outcomes implicate the potential application of EE-R and EE-L as surface disinfectants against biofilm-forming bacteria, thus promoting safer food processing practices while minimizing environmental impacts.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Priestia and Phytobacter sp. prevent membrane damage and electrolyte leakage from Capsicum annuum L. seeds subjected to sub-optimal temperature stress. Priestia和Phytobacter sp.可以防止辣椒种子在次优温度胁迫下膜损伤和电解质泄漏。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf033

Prima Bagui, Poulomi Pal, Nipa Biswas, Bratati Chowdhury, Binayak Chakraborty, Prithwiraj Dey, Kapudeep Karmakar

{"title":"Priestia and Phytobacter sp. prevent membrane damage and electrolyte leakage from Capsicum annuum L. seeds subjected to sub-optimal temperature stress.","authors":"Prima Bagui, Poulomi Pal, Nipa Biswas, Bratati Chowdhury, Binayak Chakraborty, Prithwiraj Dey, Kapudeep Karmakar","doi":"10.1093/femsle/fnaf033","DOIUrl":"10.1093/femsle/fnaf033","url":null,"abstract":"Unlike the Himalayas, the sub-Himalayan zones did not experience snowfall and thus suitable for growing solanaceous vegetables. However, several cold waves have been reported to affect the district of Coochbehar (West Bengal, India), which belongs to the Cwa zone (as per Koppen's classification). Variable duration of sub-optimal soil temperature can have a detrimental effect on the growth of seedlings. Our previous study demonstrates that a constant temperature of 20°C (6 degrees below the optimal soil temperature) causes a 71% loss of vigor in seeds of solanaceous plants. Since the soil temperature is not constant diurnally, it was hypothesized that the duration of cold stress can have variable effects on vigor of Capsicum annuum L. It was observed that increasing the duration of cold stress (18 °C) up to 2 hours/day can improve the vigor but after 6 hours/day, a significant drop in vigor was observed. This was because of the cold-associated membrane damage leading to the leakage of electrolytes. To date, this stress existing in these regions has gone unnoticed. In this regard, biopriming the seeds with exopolysaccharide (EPS)-producing microbes can be useful as the EPS can form a protective layer on the seeds. Two lesser-known bacteria namely, Phytobacter and Priestia sp. were evaluated for their vigor-recovering ability. Treatment of seed with these microbes reduced the electrolyte leakage which improved the vigor under sub-optimal stress. This was also validated by fluorescent microscopy. Both these strains displayed an enhanced EPS-producing ability at 18°C which correlated with the reduced electrolyte leakage and enhanced stability of cell membrane. Such bacteria can help in promoting seed vigor under sub-optimal temperature stress.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143656389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola. 在橡胶树白粉病真菌Erysiphe quercicola中，dicer样基因是有效侵染植物所必需的。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf032

Lijuan He, Ao Guo, Xiaoli Li, Xinze Xu, Yuxiao Fang, Zhigang Li, Wenbo Liu, Xiao Li, Weiguo Miao, Daipeng Chen

{"title":"Dicer-like genes are essential for efficient plant infection in the rubber tree powdery mildew fungus Erysiphe quercicola.","authors":"Lijuan He, Ao Guo, Xiaoli Li, Xinze Xu, Yuxiao Fang, Zhigang Li, Wenbo Liu, Xiao Li, Weiguo Miao, Daipeng Chen","doi":"10.1093/femsle/fnaf032","DOIUrl":"10.1093/femsle/fnaf032","url":null,"abstract":"The obligate parasitic fungus Erysiphe quercicola is the causal agent of powdery mildew of rubber tree (Hevea brasiliensis), posing a significant threat to the rubber tree growth and latex production. Pathogens secrete sRNAs that are crucial virulence factors capable of suppressing plant immunity. Dicer-like proteins (DCLs), as endoribonucleases, play a pivotal role in sRNA biosynthesis. However, the function of DCL in E. quercicola remains unclear. In this study, we identified and characterized two DCLs from E. quercicola, EqDCL1 and EqDCL2. Protein domain analysis and phylogenetic analysis confirmed that EqDCL1 and EqDCL2 are typical DCL proteins. Gene expression analysis revealed that EqDCL1 and EqDCL2 were induced at 10 hpi during the peak of appressorium formation. HIGS targeting EqDCL1, EqDCL2, or both EqDCL1 and EqDCL2 resulted in reduced pathogenicity of E. quercicola and increased immune responses in plants after infection. Overall, our findings demonstrate the essential role of EqDCLs during the infection process of E. quercicola, providing a foundation for further studies on RNAi mechanisms and pathogenic sRNAs in E. quercicola.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria. 一种来自山地湿地的嗜中性、降解木聚糖的植物菌——石勒斯里亚。石勒斯里亚属的修正描述。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf006

Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh

{"title":"Schlesneria sphaerica sp. nov., a neutrophilic, xylan-degrading planctomycete from a mountain wetland, and emended description of the genus Schlesneria.","authors":"Irina S Kulichevskaya, Anastasia A Ivanova, Daniil G Naumoff, Anna A Zlatogorskaya, Gennady S Kachmazov, Svetlana N Dedysh","doi":"10.1093/femsle/fnaf006","DOIUrl":"10.1093/femsle/fnaf006","url":null,"abstract":"Aerobic chemoorganotrophic planctomycetes of the genus Schlesneria colonize a wide spectrum of freshwater ecosystems. The only described species of this genus, S. paludicola, is represented by ellipsoid-shaped, moderately acidophilic bacteria isolated from acidic peat bogs. Here, we characterize a novel neutrophilic member of this genus from a mountain wetland, strain T3-172T. This isolate possesses unpigmented, non-motile, spherical cells, which grow on media containing some mono- and polysaccharides in the pH range of 6.0-7.5 and at temperatures between 15 and 37°C. Good growth is observed on xylan, one of the major plant cell wall biopolymers. The genome of strain T3-172T is 7.16 Mb in size and contains two unlinked sets of rRNA genes, 93 tRNA genes, and about 5500 potential protein-coding genes. Among the latter, the GH5 family protein is identified as the most reliable candidate for the cleavage of xylan. The 16S rRNA gene sequence of strain T3-172T displays 97.8% similarity to that of S. paludicola MPL7T, and the average nucleotide identity between the genomes of these planctomycetes is 71.6%. We, therefore, propose a novel species of the genus Schlesneria, S. sphaerica sp. nov., with strain T3-172T (=KCTC 102306T = VKM B-3856T) as the type strain.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic and metatranscriptomic analysis reveals the enzymatic mechanism of plant polysaccharide degradation through gut microbiome in plateau model animal (Ochotona curzoniae). 宏基因组和超转录组学分析揭示了高原模型动物（Ochotona curzoniae）肠道菌群降解植物多糖的酶促机制。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf045

Xueying Gan, Qiaoling Yu, Xueqian Hu, Yuan Qian, Xianxian Mu, Huan Li

{"title":"Metagenomic and metatranscriptomic analysis reveals the enzymatic mechanism of plant polysaccharide degradation through gut microbiome in plateau model animal (Ochotona curzoniae).","authors":"Xueying Gan, Qiaoling Yu, Xueqian Hu, Yuan Qian, Xianxian Mu, Huan Li","doi":"10.1093/femsle/fnaf045","DOIUrl":"10.1093/femsle/fnaf045","url":null,"abstract":"Herbivorous animals can obtain energy by decomposing plant polysaccharides through gut microbiota, but the mechanism of gut microbiota decomposing plant polysaccharides in high-altitude model animals is still unclear. Plateau pika (Ochotona curzoniae) is a key model animal native to the Qinghai-Tibet Plateau with a high intake of grass. Thus, Plateau pika is an excellent animal model for studying how herbivorous animals digest and metabolize grass polysaccharides. Here, we used 16S rDNA, 16S rRNA, metagenomic, and metatranscriptomic sequencing to characterize gut microbial composition, gene potential, and expressed function in pikas from different altitudes. Unlike total bacteria, Oscillospira and Ruminococcus were main active bacterial genera in pika's gut. The metabolic pathways of cellulose and hemicellulose were up-regulated in the middle and high-altitude groups; those genes encoding polysaccharide enzymes were enriched. Notably, the proportion of lignin metabolic genes expressed in pika's gut was the highest, followed by cellulase and hemicellulase genes. According to comparative metagenomics of different animals, the number and relative abundance of cellulase and hemicellulase genes in pika's gut were at a higher level compared with steer, etc. These results indicated that plateau pika obtained sufficient energy from grass-based diet by increasing the expression of related metabolic enzymes.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation of filamentous growth in Dekkera spp. by quorum sensing aromatic alcohols 2-phenylethanol and tryptophol. 用群体感应芳香醇、2-苯乙醇和色氨酸对赤藓丝状生长的激发。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae105

Scott J Britton, Thijs Dingemans, Lisa J Rogers, Jane S White, Dawn L Maskell

{"title":"Excitation of filamentous growth in Dekkera spp. by quorum sensing aromatic alcohols 2-phenylethanol and tryptophol.","authors":"Scott J Britton, Thijs Dingemans, Lisa J Rogers, Jane S White, Dawn L Maskell","doi":"10.1093/femsle/fnae105","DOIUrl":"10.1093/femsle/fnae105","url":null,"abstract":"Fungi from the genus Dekkera, also known as Brettanomyces, are significant contaminants in commercial beer and wine production, and when present unintentionally, these non-domesticated yeasts result in the development of undesirable sensorial characteristics, in part due to the production of volatile phenols and acetate esters. The persistence of Dekkera spp. in industrial manufacturing environments can be attributed to its strong bioadhesive properties, allowing it to attach to various surfaces and form biofilms, which often contribute to recurrent contaminations. In other fungi, the yeast-to-filamentous transition is pivotal in enhancing bioadhesive properties, a process tightly regulated by density-dependent quorum-sensing mechanisms. However, there is no documented evidence regarding the influence of fungal quorum-sensing compounds on the yeast-to-filamentous transition in Dekkera, nor is there any evidence of existing quorum-sensing circuits in this genus. In this investigation, two Dekkera spp. were cultivated on a modified nitrogen-limiting synthetic low-ammonium dextrose medium supplemented with exogenous concentrations of quorum-sensing molecules 2-phenylethanol and tryptophol. Following cultivation, whole colonies were imaged and analyzed with a whole colony filamentation algorithm to quantify their filamentation. Our results demonstrate that the quorum-sensing compounds 2-phenylethanol and tryptophol significantly promote the yeast-to-filamentous transition in Dekkera spp., underscoring the broader presence of quorum-regulated social behaviors within this genus.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological adaptations of clinical vs. indoor environmental strains of Pseudomonas aeruginosa in a hospital setting. 医院环境下铜绿假单胞菌临床与室内环境菌株的生理适应

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf027

Gabriela Vasco, Ruth Martínez, Diego Noboa, Karla Vasco, Gabriel Trueba

{"title":"Physiological adaptations of clinical vs. indoor environmental strains of Pseudomonas aeruginosa in a hospital setting.","authors":"Gabriela Vasco, Ruth Martínez, Diego Noboa, Karla Vasco, Gabriel Trueba","doi":"10.1093/femsle/fnaf027","DOIUrl":"10.1093/femsle/fnaf027","url":null,"abstract":"Pseudomonas aeruginosa is a remarkably adaptive bacterium frequently implicated in severe, sometimes fatal infections within healthcare institutions. The origins of clinical strains have generated considerable debate, suggesting that infectious variants emerge through selection from a broader environmental bacterial population. Our investigation explored the physiological differences between environmental (indoor) and clinical strains of P. aeruginosa isolated from a hospital over a year. We assessed various parameters, including survival patterns, antibiotic resistance, vulnerability to ciliate predation, bacterial antagonism, and motility. Despite the minimal incidence of patient infections during our observation, environmental P. aeruginosa was prevalent throughout the hospital during our study. Clinical strains exhibited diminished resistance to certain antibiotics, increased resistance against ciliate predators, and enhanced swarming and swimming motility compared to their environmental counterparts. Clinical strains maintained higher cell densities under starvation conditions but were outcompeted by environmental strains in a nutrient medium. In conclusion, our study suggests that P. aeruginosa clinical isolates possess unique physiological adaptations that may favor host colonization.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998. 弯曲乳酸杆菌CP2998中脂磷胆酸的化学结构。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf005

Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota

{"title":"Chemical structure of lipoteichoic acid in the probiotic strain Latilactobacillus curvatus CP2998.","authors":"Tsukasa Shiraishi, Ryo Katsuki, Hiroyuki Kumeta, Shinji Sakata, Shin-Ichi Yokota","doi":"10.1093/femsle/fnaf005","DOIUrl":"10.1093/femsle/fnaf005","url":null,"abstract":"Latilactobacillus curvatus, found in various fermented foods, is a promising probiotic with unique health benefits. Lipoteichoic acid (LTA) is a characteristic amphiphilic surface polymer of Gram-positive bacteria and exhibits immunomodulatory activities. Despite the structural diversity of LTA among different bacterial species and strains, no information is available on the chemical structure of LTA in L. curvatus. In this study, we aimed to determine the structure of LTA isolated from L. curvatus CP2998. One- and two-dimensional nuclear magnetic resonance spectra of intact LTA revealed that LTA had a glycerolphosphate polymer as a hydrophilic main chain with partial substitutions of α-linked glucose and d-alanine at the hydroxy group at position 2 of the glycerol residue. The anchor glycolipid fraction was obtained by hydrofluoric acid treatment. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry spectrum of the anchor glycolipid revealed that it contained diglucosyldiacylglycerol and diglucosylmonoacylglycerol. Our results suggest that L. curvatus CP2998 possesses a typical type I LTA structure; however, the lactic acid bacteria-specific anchor glycolipid structures, such as tri- or tetra-saccharides and three fatty acid residues, were not identified.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0