Fems Microbiology Letters最新文献_第5页

Diversity and activity of AHL-lactonases in Bacillus spp. from various environments. 不同环境下芽孢杆菌ahl内酯酶的多样性和活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf038

Iva Rosić, Ivan Nikolić, Marina Anteljević, Ivana Marić, Tamara Ranković, Slaviša Stanković, Tanja Berić, Olja Medić

{"title":"Diversity and activity of AHL-lactonases in Bacillus spp. from various environments.","authors":"Iva Rosić, Ivan Nikolić, Marina Anteljević, Ivana Marić, Tamara Ranković, Slaviša Stanković, Tanja Berić, Olja Medić","doi":"10.1093/femsle/fnaf038","DOIUrl":"10.1093/femsle/fnaf038","url":null,"abstract":"Disrupting quorum sensing (QS) pathways in animal and plant pathogenic bacteria is an effective strategy to mitigate infections without promoting antibiotic and pesticide resistance. This approach inhibits the production of virulence factors, biofilm formation, and toxin production, reducing bacterial pathogenicity. In plant health protection, Bacillus spp. are extensively researched and utilized as biocontrol agents; however, the potential of their AHL-lactonase-producing ability, which plays a key role as a QS inhibitor of Gram-negative pathogens, remains largely unexplored. This study examined the activity and diversity of QQ enzymes from Bacillus spp. isolates obtained from various natural sources, confirming their presence in previously unreported environments associated with agricultural fields (straw and manure). Our findings show that AiiA lactonase is the most dominant and highly conserved AHL-lactonase among Bacillus isolates from bulk soil, manure, and straw. Despite its sequence conservation, we observed significant variation in AiiA lactonase activities toward the N-hexanoyl-DL-homoserine lactone (C6-HSL) substrate. Furthermore, in silico analysis suggested that the Bacillus sp. YtnP lactonase may have a lower affinity for C6-HSL compared to AiiA lactonase. Finally, this research presents a selection of Bacillus isolates with high AiiA lactonase activity for potential testing against plant pathogens.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity. 位于VIM-2活性位点附近的保守辅助残基影响其金属β-内酰胺酶活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf007

Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh

{"title":"Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity.","authors":"Diamond Jain, Tejavath Ajith, Jyoti Verma, Debasmita Chatterjee, Anindya S Ghosh","doi":"10.1093/femsle/fnaf007","DOIUrl":"10.1093/femsle/fnaf007","url":null,"abstract":"Verona-integron-metallo-β-lactamase (VIM-2) is one of the most widespread class B β-lactamase responsible for β-lactam resistance. Although active-site residues help in metal binding, the residues nearing the active-site possess functional importance. Here, to decipher the role of such residues in the activity and stability of VIM-2, the residues E146, D182, N210, S207, and D213 were selected through in-silico analyses and substituted with alanine using site-directed mutagenesis. The effects of substitution mutations were assessed by comparing the changes in β-lactam susceptibility pattern of Escherichia coli host cell expressing VIM-2 and its mutated proteins. VIM-2_N210A enhanced the susceptibility of the host by ∼4-8 folds against penicillins and cephalosporins, while the expression of VIM-2_D182A radically increased the susceptibility of host. However, expression of VIM-2_E146A reduced the susceptibility of host by 2-fold. Further, proteins were purified to homogeneity, and VIM_N210A and VIM_D182A displayed reduced thermal stability than VIM-2. Moreover, in vitro catalytic efficiencies of VIM-2_D182A were drastically reduced against all the β-lactams tested whereas the same were moderately reduced for VIM-2_N210A. Conversely, the catalytic efficiency was marginally altered for VIM_E146A. Overall, we infer that both N210A and D182A substitutions negatively affect the performance of VIM-2 by influencing substrate specificity and stability, respectively.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life on the rocks: polyphasic evaluation of three epilithic cyanobacterial strains isolated from a single rock, with the description of Nostoc sikkimense sp. nov., from the northeastern region of India. 岩石上的生命：对从单一岩石中分离出的三种附石蓝藻菌株进行多相评价，并描述了来自印度东北部地区的 Nostoc sikkimense sp.

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf037

Sagarika Pal, Harsh Pant, Naresh Kumar, Priya, Shubham Singh, Nainshi Gupta, Prashant Singh

{"title":"Life on the rocks: polyphasic evaluation of three epilithic cyanobacterial strains isolated from a single rock, with the description of Nostoc sikkimense sp. nov., from the northeastern region of India.","authors":"Sagarika Pal, Harsh Pant, Naresh Kumar, Priya, Shubham Singh, Nainshi Gupta, Prashant Singh","doi":"10.1093/femsle/fnaf037","DOIUrl":"10.1093/femsle/fnaf037","url":null,"abstract":"Three epilithic cyanobacterial strains were isolated from the scrapings of a single rock surface from the Reshi River in Sikkim, India. At the time of sampling, the rock surface did not show any visible cyanobacterial growth; however, the surface of the rock was glistering. Subsequent morphological analysis indicated that two out of three strains exhibited typical Nostoc-like morphology and the third strain had cell division in multiple planes showing typical morphology of a member of the family Hapalosiphonaceae. Further, 16S rRNA gene phylogeny indicated the strains to be members of the genera Nostoc, Desmonostoc, and Westiellopsis. For species-level demarcation, additionally, 16S-23S ITS (Internal Transcribed Spacer) region analysis was performed, which indicated that the strain RESHI-1B-PS was a novel cyanobacterial lineage of the genus Nostoc, while the strains RESHI-1A-PS and RESHI-1C-PS were representatives of Desmonostoc sp. and Westiellopsis prolifica, respectively. Thus, in the current investigation, we have described an undocumented species of the cyanobacteria, which we named Nostoc sikkimense in accordance with the guidelines outlined in the International Code of Nomenclature for algae, fungi, and plants (ICN). The study also enumerates and illustrates different life cycle stages of N. sikkimense RESHI-1B-PS along with further expanding the geographic distribution of W. prolifica and its substantial ecological adaptability.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143779540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of glycolysis decreases sugar-induced cell death in Saccharomyces cerevisiae. 抑制糖酵解可降低糖诱导的酿酒酵母细胞死亡。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf026

Airat Valiakhmetov

{"title":"Suppression of glycolysis decreases sugar-induced cell death in Saccharomyces cerevisiae.","authors":"Airat Valiakhmetov","doi":"10.1093/femsle/fnaf026","DOIUrl":"10.1093/femsle/fnaf026","url":null,"abstract":"Although 30 years have passed since the description of sugar-induced cell death (SICD), the specific molecular mechanism that triggers this process remains unclear. This paper attempts to shed light on the relationship between SICD and glucose catabolism. In yeast cells, glucose is involved not only in energy-producing processes but also in the synthesis of reserve hydrocarbons. It is known that disruption of trehalose synthesis leads to significant changes in the physiology of Saccharomyces cerevisiae. The present study shows that deletion of the TPS1 gene resulted in a 44% suppression of SICD and a 75% reduction in the number of cells with excess ROS (reactive oxygen species). The suppression was comparable to the suppression of SICD (38%) and ROS (71%) with deletion of the HXK2 gene. Since HXK2 is the first enzyme in the glycolytic pathway, the effect of two other key glycolytic enzymes on SICD was tested. Deletion of the TDH3 gene (glyceraldehyde-3-phosphate dehydrogenase) resulted in a 39% suppression of SICD and ROS by 48%. Inhibition of Tdh3p with 1 mM iodoacetamide also suppressed SICD by 67% and ROS by 58%. Deletion of the PFK1 (phosphofructokinase 1) gene resulted in a complete block of SICD (97%) but unexpectedly resulted in a significant increase in the number of cells with excess ROS. The results obtained suggest that such a phenomenon as SICD is the result of an imbalance in the cellular pathways of glucose catabolism.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and expression of a hydrophobic carboxylic acid reductase from Trichoderma virens. 绿木霉疏水性羧酸还原酶的鉴定与表达。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf021

Andrew B H Goh, Jonathan G Ling, Shazilah Kamaruddin, Abdul M A Murad, Farah D Abu Bakar

{"title":"Identification and expression of a hydrophobic carboxylic acid reductase from Trichoderma virens.","authors":"Andrew B H Goh, Jonathan G Ling, Shazilah Kamaruddin, Abdul M A Murad, Farah D Abu Bakar","doi":"10.1093/femsle/fnaf021","DOIUrl":"10.1093/femsle/fnaf021","url":null,"abstract":"Carboxylic acid reductases (CARs) have been garnering attention in applications for the sustainable synthesis of aldehydes. Despite numerous discoveries, not all characteristics of CAR enzymes have been extensively studied or understood. Herein, we report the discovery and expression of a new CAR enzyme (TvirCAR2) from the ascomycetous fungus, Trichoderma virens. Tvircar2 is one of the five putative CARs identified from analyses of the T. virens genome. In silico, analyses showed that TvirCAR2 has a high hydrophobicity index and that its corresponding gene is part of a biosynthetic gene cluster predicted to synthesize hybrid polyketide synthases-nonribosomal peptide synthetase secondary metabolites. TvirCAR2 was highly expressed as soluble and insoluble forms in an Escherichia coli expression host. The solubility of the purified TvirCAR2 necessitated the addition of glycerol in the purification and assay buffers. Substrate screening via molecular docking showed that benzoic acid was a suitable substrate candidate. The TvirCAR2 enzyme catalyzed the reduction of benzoic acid with a specific activity of around 1.4 µmol/h/mg. Homologs, which are predicted to exhibit similar hydrophobicity, are the CARs from Stachybotrys bisbyi (StbB), which is involved in the production of the meroterpenoid, ilicicolin B, and Trichoderma reesei (TrCAR), which is part of a similar but still uncharacterized biosynthetic gene cluster.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surprising effects of differential loss in genome evolution: the last-one-out. 基因组进化中差异损失的惊人影响：最后一个出局。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf051

Nico Bremer, William F Martin, Mike Steel

引用次数: 0

Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T. regifaucium Amycolatopsis DSM 45072T编码decaplanin和kigamicin的NRPS和II型PKS生物合成基因簇的鉴定

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf049

Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur

{"title":"Identification of NRPS and type II PKS biosynthetic gene cluster (s) encoding decaplanin and kigamicin from Amycolatopsis regifaucium DSM 45072T.","authors":"Niyati Bisht, Shanmugam Mayilraj, Shailesh Kumar, Navjot Kaur","doi":"10.1093/femsle/fnaf049","DOIUrl":"10.1093/femsle/fnaf049","url":null,"abstract":"Amycolatopsis regifaucium, a Gram-positive actinomycete, is a prolific source of biologically active compounds, including polyphenol antibiotics like kigamicins. This study presents the draft genome of A. regifaucium DSM 45072T (= GY080T), which spans 8.28 Mbp and is assembled into 62 contigs, with annotation revealing 312 subsystems, 7966 coding genes, and 52 RNAs, with a GC content of 68.5 mol%. We found a significant genomic diversity within the genus, revealing variations in core and accessory genomic elements across species. Multiple biosynthetic gene clusters have been identified, including a previously unidentified glycopeptide antibiotic gene cluster and a type II polyketide synthase gene cluster, highlighting the organism's metabolic versatility and potential for the biosynthesis of novel natural products. Our analysis confirmed the production of decaplanin, an antibiotic previously attributed to Amycolatopsis decaplanina DSM 44594T. Correspondingly, the gene cluster responsible for decaplanin biosynthesis is identified in A. regifaucium DSM 45072T and A. decaplanina DSM 44594T. Additionally, a putative type II PKS gene cluster is predicted within the glycopeptide antibiotic-producing clade (Cluster A) of the genus Amycolatopsis. Genomics insights from A. regifaucium DSM 45072T represent it as a promising genetic resource with significant implications for biotechnological and pharmaceutical innovation, particularly in discovering and developing novel antimicrobial agents.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial susceptibility and genomic characterization of Vibrio cholerae non-O1/non-O139 isolated from clinical and environmental samples in Jiaxing City, China. 嘉兴市临床和环境中分离的非o1 /非o139型霍乱弧菌的药敏及基因组特征

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf009

Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen

{"title":"Antimicrobial susceptibility and genomic characterization of Vibrio cholerae non-O1/non-O139 isolated from clinical and environmental samples in Jiaxing City, China.","authors":"Miaomiao Jia, Ping Li, Yong Yan, Xuejuan Liu, Lei Gao, Guoying Zhu, Zhongwen Chen","doi":"10.1093/femsle/fnaf009","DOIUrl":"10.1093/femsle/fnaf009","url":null,"abstract":"Non-O1/non-O139 (NOVC) strains inhabit aquatic environments and sporadically induce human illnesses. This study involved the virulence and antimicrobial genetic characterization of 176 NOVC strains, comprising 25 from clinical samples and 151 from environmental sources, collected between 2021 and 2023. The antimicrobial susceptibility of the examined NOVC population was predominantly high, exhibiting only poor susceptibility to colistin, with 89.2% resistance. The examination of virulence genes revealed that the majority of strains were positive for glucose metabolism (als gene) (169/176, 96.0%). Through multilocus sequence typing, the 176 NOVC strains were categorised into 121 sequence types, 79 of which were novel. NOVC strains demonstrate significant genetic variability and frequently engage in recombination. This work offers genetic characterization of the pathogenicity and antimicrobial resistance of a NOVC community. Our findings offer insights that may aid in the development of preventative and treatment methods for this pathogen.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic analysis of the mode of action and mode of resistance of fusidic acid against Staphylococcus aureus. 氟西地酸对金黄色葡萄球菌作用模式（MoA）和耐药模式（MoR）的代谢分析。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf011

Dan Luo, Juanjuan Ma, Weile Xie, Zhe Wang

{"title":"Metabolic analysis of the mode of action and mode of resistance of fusidic acid against Staphylococcus aureus.","authors":"Dan Luo, Juanjuan Ma, Weile Xie, Zhe Wang","doi":"10.1093/femsle/fnaf011","DOIUrl":"10.1093/femsle/fnaf011","url":null,"abstract":"Understanding bacterial responses to antibiotics is essential for identifying resistance mechanisms and developing novel therapies. This study evaluated the resistance of Staphylococcus aureus (S. aureus) to fusidic acid (FD) in 100 patients with skin and soft tissue infections (SSTIs), revealing susceptibility to FD despite resistance to other antibiotics. Through adaptive laboratory evolution, we developed a highly FD-resistant strain, E10, and identified three gene mutations (fusA, BPENGOFF-00211, and rplF) using whole-genome sequencing. The fusA mutation was the primary contributor to resistance. Furthermore, the evolved fusA mutant strain (H457Y) displayed impaired coagulation function and reduced growth rates. We also analyzed the metabolomic profiles of ancestral ATCC 25923 and evolved E10 strains, both treated and untreated with FD, revealing that the fusA gene can independently induce metabolic reprogramming. These changes primarily impacted pathways involved in central carbon metabolism, nucleotide metabolism, and amino acid synthesis. This study highlights the complexity of FD resistance in S. aureus and offers insights into the metabolic pathways associated with antibiotic resistance.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of antibiotic-resistant Escherichia coli harbouring extended-spectrum beta-lactamase (ESBL) in raw chicken meat sold at retail in Klang Valley, Malaysia. 马来西亚巴生谷零售的生鸡肉中含有广谱β -内酰胺酶（ESBL）的耐抗生素大肠杆菌的高发率

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf039

Sur Guat Goh, Son Radu, Yuli Haryani, Lee Epeng, Mahmud Ab Rashid Nor-Khaizura, Noor Azira Abdul-Mutalib, Hanan Hasan

{"title":"High prevalence of antibiotic-resistant Escherichia coli harbouring extended-spectrum beta-lactamase (ESBL) in raw chicken meat sold at retail in Klang Valley, Malaysia.","authors":"Sur Guat Goh, Son Radu, Yuli Haryani, Lee Epeng, Mahmud Ab Rashid Nor-Khaizura, Noor Azira Abdul-Mutalib, Hanan Hasan","doi":"10.1093/femsle/fnaf039","DOIUrl":"10.1093/femsle/fnaf039","url":null,"abstract":"Chicken is the most widely consumed meat in Malaysia as it is abundant, provides good nutrient and taste, and available at an affordable price. However, it is known to harbour various foodborne pathogens including faecal microorganism, Escherichia coli. There are various routes and factors that can cause contamination of E. coli in chicken. Furthermore, numerous reports have shown that over the past decades, the trends of antimicrobial resistance among foodborne pathogens have been increasing rapidly. Therefore, the present work aimed to assess the prevalence of E. coli contamination by examining various contributing factors and its antibiotic resistance in raw chicken meat sold in Klang Valley, Malaysia. Results showed that 74% of the samples were contaminated with E. coli with wet markets showing higher prevalence (17%) of E. coli than in hypermarkets. Univariate analysis within the same risk factor showed that packaging process, storage temperature, and antibiotics had significant effects on the prevalence of E. coli (∼ 6.097 log CFU/g). The E. coli loads were significantly influenced by market type and storage temperature as validated by Mann-Whitney tests. All E. coli isolates displayed multiple antibiotic resistance (MAR) index ranging from 0.33 to 1.00, and 35 E. coli isolates showed the highest MAR index (1.00), being resistant to 12 antibiotics. Furthermore, 90% of E. coli isolates contained extended-spectrum beta-lactamase genotypes that can subvert potent antibiotic, beta-lactam. The findings from the present work would help reduce the risk of foodborne illnesses by identifying the risk factors associated with E. coli prevalence in chicken and provide the basis to revise guidelines on antibiotic use in livestock to reduce antimicrobial resistance.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0