Fems Microbiology Letters最新文献_第4页

SoxR-dependent regulation of sodA1 and its impact on Stenotrophomonas maltophilia survival under external oxidative stress. soxr依赖性sodA1调控及其对嗜麦芽窄养单胞菌外部氧化应激存活的影响

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae112

Suparat Giengkam, Nisanart Charoenlap, Wirongrong Whangsuk, Kisana Bhinija, Skorn Mongkolsuk, Paiboon Vattanaviboon

{"title":"SoxR-dependent regulation of sodA1 and its impact on Stenotrophomonas maltophilia survival under external oxidative stress.","authors":"Suparat Giengkam, Nisanart Charoenlap, Wirongrong Whangsuk, Kisana Bhinija, Skorn Mongkolsuk, Paiboon Vattanaviboon","doi":"10.1093/femsle/fnae112","DOIUrl":"10.1093/femsle/fnae112","url":null,"abstract":"Stenotrophomonas maltophilia is an emerging global opportunistic pathogen that causes nosocomial infections. We demonstrated that the superoxide stress-sensing transcriptional regulator SoxR directly modulated the expression of an operon encompassing sodA1 (encoding manganese-containing superoxide dismutase) and fre (encoding putative flavin reductase) by directly binding to the operator site, which was located between the -35 and -10 motifs of the sodA1 promoter. It is known that upon exposure to the superoxide generators/redox-cycling drugs, the SoxR, which is bound to the operator site, became oxidized. This oxidation causes a conformational change of SoxR to an active form, enabling the upregulation of sodA1-fre gene expression. A ΔsodA1 was constructed, and the mutant showed enhanced sensitivity to the redox-cycling drugs, including menadione, plumbagin, and methyl viologen (paraquat), relative to its parental strain K279a. Thus, sodA1 may play a role in the survival of S. maltophilia under superoxide stress during either its saprophyte stage (e.g. exposure to redox-cycling drugs) or host-pathogen interactions.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa. α-生育酚对金黄色葡萄球菌、奇异变形杆菌和铜绿假单胞菌的生物膜破坏活性。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf020

Pui Yee Leong, Wei Qi Tan, Wee Sim Choo

{"title":"Biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa.","authors":"Pui Yee Leong, Wei Qi Tan, Wee Sim Choo","doi":"10.1093/femsle/fnaf020","DOIUrl":"10.1093/femsle/fnaf020","url":null,"abstract":"Antibiotic resistance and the persistence of sessile cells within biofilms complicate the eradication of biofilm-related infections using conventional antibiotics. This highlights the necessity for alternate therapy methods. The objective of this study was to investigate the biofilm destruction activity of α-tocopherol against Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa on polystyrene. α-Tocopherol showed significant biofilm destruction activity on the pre-formed biofilms of S. aureus (45%-46%), Pr. mirabilis (42%-54%), and Ps. aeruginosa (28%). Resazurin assay showed that α-tocopherol disrupted all bacterial biofilms without interfering with their cell viability. Scanning electron microscope images showed lower bacterial cell count and less compacted cell aggregates on polystyrene surfaces after treatment with α-tocopherol. This study demonstrated the biofilm destruction activity of α-tocopherol against S. aureus, Pr. mirabilis, and Ps. aeruginosa. α-Tocopherol could potentially be used to decrease biofilm-associated infections of these bacteria.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of Lactobacillus species from gut microbiota of Aegiale hesperiaris (Lepidoptera: Hesperiidae) larvae. 猕猴桃鳞翅目：猕猴桃科幼虫肠道菌群中乳杆菌的分离与鉴定。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf015

Ericka Denice Herrera-Cardoso, Karen Alejandra Tapia-Cervantes, Jonathan Cepeda-Negrete, Santiago Gutiérrez-Vargas, Ma Fabiola León-Galván

{"title":"Isolation and identification of Lactobacillus species from gut microbiota of Aegiale hesperiaris (Lepidoptera: Hesperiidae) larvae.","authors":"Ericka Denice Herrera-Cardoso, Karen Alejandra Tapia-Cervantes, Jonathan Cepeda-Negrete, Santiago Gutiérrez-Vargas, Ma Fabiola León-Galván","doi":"10.1093/femsle/fnaf015","DOIUrl":"10.1093/femsle/fnaf015","url":null,"abstract":"Entomophagy, the practice of consuming insects, is a global tradition. In Mexico, one of the most notable and widely consumed insects is the larva of Aegiale hesperiaris. This insect feeds on the leaves of various Agave species with high polysaccharide content, suggesting their potential role as prebiotics for the intestinal microbiota, particularly lactic acid bacteria (LAB). LAB are recognized for their use as probiotics in foods due to their health-promoting capabilities. In this study, LAB from the intestinal microbiota of A. hesperiaris larvae were isolated and characterized, utilizing 16S rRNA gene identification. The analysis revealed three bacterial species from the Lactobacillaceae family, indicating a close symbiotic relationship with the insect. This suggests a significant impact on carbohydrate and protein metabolism, vitamin synthesis, and amino acid production, contributing to the high nutritional value of this edible insect. The study provides insights into the bacteria within the digestive tract of A. hesperiaris larvae and their role in enhancing the nutritional value of this edible insect. Additionally, it establishes a foundation for future research on the ecological roles and potential biotechnological benefits of these bacteria in the food industry and the development of therapies for various conditions and diseases.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143064836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Concern: Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota. 表达关切：从人类肠道微生物群中分离出的新细菌物种 Limosilactobacillus caccae sp.

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf014

引用次数: 0

Correction to: Molecular hydrogen from water radiolysis as an energy source for bacterial growth in a basin containing irradiating waste. 更正：在含有辐照废物的水池中，作为细菌生长能量来源的水辐射分解产生的氢分子。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf003

引用次数: 0

The adoption of a superhero bacteria: Escherichia coli and its adventures in microbiology education. 超级英雄细菌的采用：大肠杆菌及其在微生物学教育中的冒险。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf042

Isabelle Carolina Cotrim Gozzi, Bruna Gennari Rosa, Isadora Medeiros, Beatriz Dinat Labone Silva, Giovanna Matos Rodrigues, Fernanda Ayumi Nagay Yoshihara, Giovanna Tresso Custódio, José Ricardo Ferreira de Lucena, Lara Nardi Baroni, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira

{"title":"The adoption of a superhero bacteria: Escherichia coli and its adventures in microbiology education.","authors":"Isabelle Carolina Cotrim Gozzi, Bruna Gennari Rosa, Isadora Medeiros, Beatriz Dinat Labone Silva, Giovanna Matos Rodrigues, Fernanda Ayumi Nagay Yoshihara, Giovanna Tresso Custódio, José Ricardo Ferreira de Lucena, Lara Nardi Baroni, Ana Carolina Ramos Moreno, Rita de Cássia Café Ferreira","doi":"10.1093/femsle/fnaf042","DOIUrl":"https://doi.org/10.1093/femsle/fnaf042","url":null,"abstract":"Here, we present a case study of undergraduate biomedical students engaging in the Adopt a Bacterium active methodology. The study was designed and authored by students, associating heroes, and antiheroes with Escherichia coli to highlight its dual role in human health. By integrating elements of geek culture, we developed multimedia educational content, including social media posts, interactive chalkboard presentation, gamified seminar, and a comic book. This creative approach enabled a deeper understanding of microbiological concepts, improving communication skills, and stimulating engagement beyond the classroom. Our experience highlights the potential of integrating storytelling and visual media into science education to enhance motivation, autonomy, and practical/theoretical skills, alongside teamwork and science-based communication. Furthermore, we recognized that scientific dissemination involves making science accessible, empowering us to learn and share knowledge with future generations, thereby fostering a ripple effect in scientific literacy. By transforming abstract microbiological concepts into accessible and relatable narratives, the Adopt a Bacterium demonstrates the power of active methodologies in education and public engagement.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using core genome and machine learning for serovar prediction in Salmonella enterica subspecies I strains. 利用核心基因组和机器学习预测肠沙门氏菌I亚种菌株的血清型。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf040

Xiang Li, Adelumola Oladeinde, Michael Rothrock, Tae Jung Chung, Walid Ghazi Al Hakeem

{"title":"Using core genome and machine learning for serovar prediction in Salmonella enterica subspecies I strains.","authors":"Xiang Li, Adelumola Oladeinde, Michael Rothrock, Tae Jung Chung, Walid Ghazi Al Hakeem","doi":"10.1093/femsle/fnaf040","DOIUrl":"https://doi.org/10.1093/femsle/fnaf040","url":null,"abstract":"This study presents a dual investigation of Salmonella enterica subspecies I, focusing on serovar prediction and core genome characteristics. We utilized two large genomic datasets (panX and NCBI Pathogen Detection) to test machine learning methods for predicting Salmonella serovars based on genomic differences. Among the four tested algorithms, the Random Forest model demonstrated higher performance, achieving 90.3% accuracy with the panX dataset and 95.3% with the NCBI dataset, particularly effective when trained on >50% of available data. When combined with hierarchical clustering validation, our approach achieved 100% prediction accuracy on the simulated data. Parallel analysis of panX core genome characteristics revealed that pathogenicity-related genes (including sseA, invA, mgtC, phoP, phoQ, and sitA) exhibited similar phylogenetic topologies distinct from the core genome phylogenetic tree, suggesting shared evolutionary histories. Notably, all identified core antibiotic resistance genes and virulence factors showed evidence of negative selection, indicating their essential role in bacterial survival. This study not only presents a promising machine learning-based alternative for Salmonella serovar classification, particularly valuable when analyzing newly identified serovars alongside known reference strains but also provides insights into the evolutionary dynamics of core virulence-associated genes, contributing to our understanding of Salmonella genomic architecture and pathogenicity.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":"372 ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro enzyme characterization and several inhibitors for monkeypox virus core protease I7L. 猴痘病毒核心蛋白酶I7L的体外酶学特性及几种抑制剂。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf008

Lin Wei, Yuqi Wu, Shuai Li, Jun Weng, Miaomiao Geng, Meng Mei, Zigong Wei

引用次数: 0

Distribution and functional analysis of two types of quorum sensing gene pairs, glaI1/glaR1 and glaI2/glaR2, in Burkholderia gladioli. 两类群体感应基因glaI1/ gl1和glaI2/ gl2在剑兰伯克霍尔德菌中的分布及功能分析

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnae117

Kazumi Takita, Nobutaka Someya, Tomohiro Morohoshi

{"title":"Distribution and functional analysis of two types of quorum sensing gene pairs, glaI1/glaR1 and glaI2/glaR2, in Burkholderia gladioli.","authors":"Kazumi Takita, Nobutaka Someya, Tomohiro Morohoshi","doi":"10.1093/femsle/fnae117","DOIUrl":"10.1093/femsle/fnae117","url":null,"abstract":"Burkholderia gladioli produces a yellow-pigmented toxin called toxoflavin, and causes disease on a variety of plants. Previous studies have suggested that the pathogenicity of B. gladioli is regulated by an N-acyl-l-homoserine lactone (AHL)-mediated quorum sensing (QS) system. In this study, complete genome sequencing revealed that B. gladioli pv. gladioli MAFF 302385 possesses two types of AHL synthase and AHL receptor gene pairs: glaI1/glaR1 and glaI2/glaR2. Disruption of QS genes revealed that the glaI1/glaR1 QS system regulated swarming motility, biofilm formation, and colony formation via N-octanoyl-l-homoserine lactone. Although Escherichia coli harboring glaI2 produced N-(3-hydroxyoctanoyl)-l-homoserine lactone and N-(3-hydroxydecanoyl)-l-homoserine lactone, the expression of glaI2 was not confirmed in MAFF 302385 cells. We also found that toxoflavin production was regulated by the glaI1/glaR1 QS system in liquid medium, but not on agar medium. When pathogenicity tests were performed on gladiolus leaves, the wild-type and QS mutants showed a similar level of disease. Our results demonstrated that only the glaI1/glaR1-mediated QS system is active in MAFF 302385, but major virulence factors, especially toxoflavin, are not completely dependent on the QS system.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival of Salmonella during dry salting process of pork feet. 猪脚干腌过程中沙门氏菌的存活。

IF 2.2 4区生物学

Fems Microbiology Letters Pub Date : 2025-01-10 DOI: 10.1093/femsle/fnaf034

Rafaela Busnello, Luisa Aneiros Gené, Mariane Ferreira Silva, Fabiola Ayres Cacciatore, Nathanyelle Soraya Martins de Aquino, Eduardo Cesar Tondo

{"title":"Survival of Salmonella during dry salting process of pork feet.","authors":"Rafaela Busnello, Luisa Aneiros Gené, Mariane Ferreira Silva, Fabiola Ayres Cacciatore, Nathanyelle Soraya Martins de Aquino, Eduardo Cesar Tondo","doi":"10.1093/femsle/fnaf034","DOIUrl":"10.1093/femsle/fnaf034","url":null,"abstract":"Pork feet (PF) contaminated with Salmonella are often discarded by the food industry because heat treatment is not economically viable for these products. Dry salting can be an alternative to heat treatment if it can significantly reduce Salmonella populations. The aim of this study was to assess the Salmonella's survival in PF during dry salting process. Firstly, Salmonella was quantified in contaminated PF from the food industry. Thereafter, PF samples were artificially contaminated with three levels of Salmonella (3, 6, and 8 log CFU/g) and stored at 10°C for 30 days. Salmonella survival was assessed by counts on xylose lysine deoxycholate agar, mannitol lysine crystal-violet brilliant-green agar, and by the 3M™ 2 Molecular Detection Assay (MDA). After 18 days of salting, the 3 log CFU/g inoculum was no longer detected by the cultural and detection methods. The 6 and 8 log CFU/g inoculums were not detected on the agar plates after 26 days of salting but were still positive by MDA. Considering that Salmonella counts in contaminated PF in the food industry were 1.56 log CFU/g and that populations of 3-8 log CFU/g of Salmonella were reduced to unculturable levels after dry salting, this process could contribute to the control of Salmonella in PF.","PeriodicalId":12214,"journal":{"name":"Fems Microbiology Letters","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0