卤虫 Haloferax volcanii 中一种新型酮异酵素酶的鉴定和表征

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Marius Ortjohann, Peter Schönheit
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引用次数: 0

摘要

酮合酶(KHK)催化果糖的 ATP 依赖性磷酸化,形成 1-磷酸果糖和 ADP。对真核生物,特别是人类和其他脊椎动物中的这种酶进行了深入研究,但尚未在细菌和古细菌中发现同源物。在这里,我们报告了从卤代古细菌 Haloferax volcanii(HvKHK)中鉴定出的一种新型 KHK。编码基因 khk 被鉴定为 HVO_1812。该基因表达为 90 kDa 的同源二聚体蛋白,可催化果糖磷酸化,其 Vmax 值为 59 U/mg ,对 ATP 和果糖的表观 KM 值分别为 0.47 mM 和 1.29 mM。HvKHK 的同源物仅在少数卤代古细菌和嗜卤细菌中发现。该蛋白与真核生物中的KHKs序列同一性较低,系统进化分析表明,半知菌类的KHKs与真核生物的KHKs在很大程度上是分离的。这是首次发现原核生物中的 KHKs,它们在核糖激酶/pfkB 超家族中形成了一个新的糖激酶群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and characterization of a novel type of ketohexokinase from the haloarchaeon Haloferax volcanii
Ketohexokinase (KHK) catalyzes the ATP-dependent phosphorylation of fructose, forming fructose-1-phosphate and ADP. The enzyme is well studied in Eukarya, in particular in human and other vertebrates, but homologs have not been identified in Bacteria and Archaea. Here we report the identification of a novel type of KHK from the haloarchaeon Haloferax volcanii (HvKHK). The encoding gene khk was identified as HVO_1812. The gene was expressed as 90 kDa homodimeric protein, catalyzing the phosphorylation of fructose with a Vmax value of 59 U/mg and apparent KM values for ATP and fructose of 0.47 mM and 1.29 mM, respectively. Homologs of HvKHK were only identified in few haloarchaea and halophilic Bacteria. The protein showed low sequence identity to characterized KHKs from eukaryotes and phylogenetic analyses indicate that haloarchaeal KHKs are largely separated from eukaryal KHKs. This is the first report of the identification of KHKs in prokaryotes that form a novel cluster of sugar kinases within the ribokinase/pfkB superfamily.
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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