Developmental cell最新文献_第4页

Combinatorial BMP4 and activin direct the choice between alternate routes to endoderm in a stem cell model of human gastrulation. 在人原肠胚干细胞模型中，组合BMP4和激活素指导通往内胚层的替代途径的选择。

IF 8.7 1区生物学

Developmental cell Pub Date : 2025-09-09 DOI: 10.1016/j.devcel.2025.08.009

Oliver C K Inge, Elias Copin, Jake Cornwall-Scoones, Borzo Gharibi, Irene Rodriguez-Hernandez, Pablo Soro-Barrio, Molly Strom, Probir Chakravarty, James Briscoe, Silvia D M Santos

{"title":"Combinatorial BMP4 and activin direct the choice between alternate routes to endoderm in a stem cell model of human gastrulation.","authors":"Oliver C K Inge, Elias Copin, Jake Cornwall-Scoones, Borzo Gharibi, Irene Rodriguez-Hernandez, Pablo Soro-Barrio, Molly Strom, Probir Chakravarty, James Briscoe, Silvia D M Santos","doi":"10.1016/j.devcel.2025.08.009","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.009","url":null,"abstract":"Lineage specification requires accurate interpretation of multiple signaling cues. However, how combinatorial signaling histories influence fate outcomes remains unclear. We combined single-cell transcriptomics, live-cell imaging, and mathematical modeling to explore how activin and bone morphogenetic protein 4 (BMP4) guide fate specification during human gastrulation. We see that these signals interact both synergistically and antagonistically to drive fate decisions. We find that definitive endoderm arises from lineage convergence: a direct route from pluripotency and an indirect route via a mesoderm progenitor state. Cells pass through temporal windows of signaling competency, and the relative concentration of activin and BMP4 dictates the trajectory choice. The efficiency between routes is underpinned by a dual role of BMP4 in inducing mesoderm genes while promoting pluripotency exit. This work underscores that the combination of signals a cell is exposed to not only directs its final fate but also the developmental route taken, suggesting lineage convergence enhances robustness in fate specification.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A pathological role of O-GlcNAcylation-driven TR11B production and function in lung adenocarcinoma o - glcn酰化驱动TR11B产生和功能在肺腺癌中的病理作用

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-09 DOI: 10.1016/j.devcel.2025.08.010

Shiyu Qiu, Lifang Ma, Keke Yu, Xin Xu, Xiao Zhang, Wenjun Yu, Kai Wang, Xiaoting Tian, Yayou Miao, Yikun Wang, Wanxin Guo, Xiangfei Xue, Jiangtao Cui, Xuewen Yu, Rui Kang, Qianjun Zhou, Yongchun Yu, Daolin Tang, Jiayi Wang

{"title":"A pathological role of O-GlcNAcylation-driven TR11B production and function in lung adenocarcinoma","authors":"Shiyu Qiu, Lifang Ma, Keke Yu, Xin Xu, Xiao Zhang, Wenjun Yu, Kai Wang, Xiaoting Tian, Yayou Miao, Yikun Wang, Wanxin Guo, Xiangfei Xue, Jiangtao Cui, Xuewen Yu, Rui Kang, Qianjun Zhou, Yongchun Yu, Daolin Tang, Jiayi Wang","doi":"10.1016/j.devcel.2025.08.010","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.010","url":null,"abstract":"Cytokines link inflammation to tumorigenesis, but the role of post-translational modifications in regulating their function within the extra-tumoral environment remains poorly defined. Here, we identify tumor-derived tumor necrosis factor (TNF) receptor superfamily member 11B (TR11B) as a key driver of lung adenocarcinoma (LUAD) progression and therapeutic resistance. Mechanistically, O-GlcNAc transferase (OGT)-mediated O-GlcNAcylation at serine 151 stabilizes TR11B and facilitates its interaction with the membrane protein EPS15 homology domain-containing protein 1 (EHD1), promoting cyclin dependent kinase 2 (CDK2) phosphorylation and cell cycle progression. Clinically, elevated O-GlcNAcylated TR11B correlates with advanced LUAD. Genetic deletion of Ogt suppresses tumor development in LUAD mouse models. Importantly, celecoxib, an U.S. Food and Drug Administration (FDA)-approved drug, inhibits O-GlcNAcylation and exerts antitumor effects. These findings reveal a pathological role for cytokine O-GlcNAcylation in LUAD and identify this axis as a potential therapeutic target.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"17 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SUMOylation of the transcription factor Etv1 in cancer stem cells induces tumorigenesis of non-stem cancer cells 肿瘤干细胞中转录因子Etv1的sumo化可诱导非干细胞肿瘤的发生

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-08 DOI: 10.1016/j.devcel.2025.06.023

Yuan Chen

引用次数: 0

Novel bacterial strategy to hijack plant immunity through metabolic manipulation 通过代谢操纵劫持植物免疫的新细菌策略

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-08 DOI: 10.1016/j.devcel.2025.08.008

Ran Lu, Elwira Smakowska-Luzan

引用次数: 0

Defining breast epithelial cell types in the single-cell era 单细胞时代乳腺上皮细胞类型的界定

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-08 DOI: 10.1016/j.devcel.2025.06.032

G. Kenneth Gray, Eric G. Carlson, Tatyana Lev, Bailey Marshall, Austin D. Reed, Alex P. Sánchez-Covarrubias, Alecia-Jane Twigger, Aleix Puig-Barbe, Aatish Thennavan, Ayodele Omotoso, Lyndsay M. Murrow, Deeptiman Chatterjee, Siyuan He, Sara Pensa, Brian Aevermann, Norbert K. Tavares, Natalie Chen, Jason A. Hilton, Kerrigan Blake, Yunlong Liu, Walid T. Khaled

引用次数: 0

Phosphorylation dynamics of RAF12 and PP2C control SnRK2 activity under hyperosmotic stress in Arabidopsis 高渗胁迫下，拟南芥中RAF12和PP2C的磷酸化动态调控SnRK2活性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-08 DOI: 10.1016/j.devcel.2025.08.018

Xiliang Liao, Wei Fan, Xiruo Wang, Qin Yu, Siyu Chen, Yaping Zhao, Xiyu Bai, Fengsong Liu, Peng Zhang, Zixing Li

引用次数: 0

A bridge too far: Identification of bridge cell states as drivers of plasticity in neuroblastoma 桥过远：桥细胞状态作为神经母细胞瘤可塑性驱动因素的鉴定

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-08 DOI: 10.1016/j.devcel.2025.06.010

Lisa B. Earnest-Noble, Bradley J. Goldstein

引用次数: 0

Morphogenesis and regeneration share a conserved core transition cell state program that controls lung epithelial cell fate 形态发生和再生共享一个保守的核心转化细胞状态程序，控制肺上皮细胞的命运

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-05 DOI: 10.1016/j.devcel.2025.08.014

Xiangyi Ke, Benjamin van Soldt, Lukas Vlahos, Yizhuo Zhou, Jun Qian, Pasquale Laise, Joel George, Claudia Capdevila, Ian Glass, Kelley Yan, Andrea Califano, Wellington V. Cardoso

引用次数: 0

Preventing CpG hypermethylation in oocytes safeguards mouse development 防止卵母细胞CpG超甲基化可保护小鼠发育

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-02 DOI: 10.1016/j.devcel.2025.08.005

Yumiko K. Kawamura, Evgeniy A. Ozonov, Panagiotis Papasaikas, Takashi Kondo, Nhuong V. Nguyen, Michael B. Stadler, Sebastien A. Smallwood, Haruhiko Koseki, Antoine H.F.M. Peters

{"title":"Preventing CpG hypermethylation in oocytes safeguards mouse development","authors":"Yumiko K. Kawamura, Evgeniy A. Ozonov, Panagiotis Papasaikas, Takashi Kondo, Nhuong V. Nguyen, Michael B. Stadler, Sebastien A. Smallwood, Haruhiko Koseki, Antoine H.F.M. Peters","doi":"10.1016/j.devcel.2025.08.005","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.005","url":null,"abstract":"Except for regulatory CpG-island sequences, genomes of most mammalian cells are widely DNA-methylated. In oocytes, though, DNA methylation (DNAme) is largely confined to transcribed regions. The mechanisms restricting de novo DNAme in oocytes and their relevance thereof for zygotic genome activation and embryonic development are largely unknown. Here we show that KDM2A and KDM2B, two histone demethylases, prevent genome-wide accumulation of histone H3 lysine 36 di-methylation, thereby impeding DNMT3A-catalyzed DNAme. We demonstrate that aberrant DNAme at CpG islands inherited from Kdm2a/Kdm2b double-mutant oocytes represses gene transcription in two-cell embryos. Aberrant maternal DNAme impairs pre-implantation embryonic development, which is suppressed by Dnmt3a deficiency during oogenesis. Hence, KDM2A/KDM2B are essential for confining the oocyte methylome, thereby conferring competence for early embryonic development. Our research implies that the reprogramming capacity eminent to early embryos is insufficient for erasing aberrant DNAme from maternal chromatin, and that early development is susceptible to gene dosage haplo-insufficiency effects.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"28 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144928558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient stem cell-derived mouse embryo models for environmental studies 用于环境研究的高效干细胞衍生小鼠胚胎模型

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-28 DOI: 10.1016/j.devcel.2025.08.004

Victoria Jorgensen, Min Bao, Sergi Junyent, Christoph M. Häfelfinger, Laura Amaya, Zhaodi Liao, Brian A. Williams, Dong-Yuan Chen, Amanda Wu, Matt Thomson, Magdalena Zernicka-Goetz

{"title":"Efficient stem cell-derived mouse embryo models for environmental studies","authors":"Victoria Jorgensen, Min Bao, Sergi Junyent, Christoph M. Häfelfinger, Laura Amaya, Zhaodi Liao, Brian A. Williams, Dong-Yuan Chen, Amanda Wu, Matt Thomson, Magdalena Zernicka-Goetz","doi":"10.1016/j.devcel.2025.08.004","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.004","url":null,"abstract":"Blastoids are stem cell-derived structures that mimic natural blastocysts by incorporating all three lineages: trophectoderm, epiblast, and primitive endoderm. However, current methods often yield incomplete structures that fail to cavitate or to form a proper primitive endoderm. To overcome these limitations, we develop a modular approach by aggregating three murine stem cell types: embryonic stem cells (ESCs), ESCs with inducible GATA4 expression (iG4-ESCs), and trophoblast stem cells (TSCs). This method yields cavitated blastocyst-like structures—termed iG4-blastoids—with approximately 80% efficiency. Single-cell RNA sequencing confirms their close resemblance to mature mouse blastocysts. Notably, culturing iG4-blastoids without FGF4 enhances specification of the invasive mural trophectoderm, and approximately 12% of structures undergo post-implantation-like morphogenesis in vitro. Using this model, we show that caffeine, alcohol, nicotine, and amino acid variations affect iG4-blastoids and natural embryos similarly, underscoring their utility as a robust model for investigating the impact of diverse environmental factors on embryogenesis.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"52 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0