Developmental cell最新文献_第10页

Phosphorylation dynamics of RAF12 and PP2C control SnRK2 activity under hyperosmotic stress in Arabidopsis 高渗胁迫下，拟南芥中RAF12和PP2C的磷酸化动态调控SnRK2活性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-23 DOI: 10.1016/j.devcel.2025.05.011

Xiliang Liao, Wei Fan, Xiruo Wang, Qin Yu, Siyu Chen, Yaping Zhao, Xiyu Bai, Fengsong Liu, Peng Zhang, Zixing Li

{"title":"Phosphorylation dynamics of RAF12 and PP2C control SnRK2 activity under hyperosmotic stress in Arabidopsis","authors":"Xiliang Liao, Wei Fan, Xiruo Wang, Qin Yu, Siyu Chen, Yaping Zhao, Xiyu Bai, Fengsong Liu, Peng Zhang, Zixing Li","doi":"10.1016/j.devcel.2025.05.011","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.011","url":null,"abstract":"Hyperosmotic stress rapidly induces the activation of SNF1-related protein kinases 2 (SnRK2s) that orchestrate plant adaptive responses. However, prolonged activation can unbalance cellular homeostasis. Molecular mechanisms that manage the activation and subsequent deactivation of SnRK2s during osmotic stress signaling are poorly understood. Our findings suggest that type 2C protein phosphatases—ABI1, ABI2, HAI1, and HAI2—cooperatively suppress SnRK2 activities in Arabidopsis. Notably, abi1abi2hai1hai2 quadruple mutant displays reduced hyperosmotic stress sensitivity and partially constitutive stress responses even under normal conditions. We also discovered that B2 Raf-like MAPKKK (RAF12) inhibits HAI2 phosphatase activities through the direct phosphorylation of HAI2, releasing the SnRK2 inhibition. Interestingly, upon hyperosmotic stress, RAF12 rapidly forms reversible condensates. RAF12 condensation, driven by its intrinsically disordered region, potentially facilitates RAF12 kinase activation. Our research elucidates that the RAF-PP2C-SnRK2 phosphorylation switch is involved in perceiving hyperosmotic stress, initiating and amplifying osmotic stress signaling, and subsequently shaping plant adaptive responses.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"70 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144341285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoskeletal crosstalk in macrophages: Vimentin drives ECM remodeling in tumor invasion and tissue plasticity 巨噬细胞中的细胞骨架串扰：Vimentin驱动肿瘤侵袭和组织可塑性中的ECM重塑

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-23 DOI: 10.1016/j.devcel.2025.05.015

John E. Eriksson

引用次数: 0

How plants respond to wheat stripe rust: Dual control of an NLR protein involving wheat ankyrin repeat protein 植物如何应对小麦条锈病：涉及小麦锚蛋白重复序列蛋白的NLR蛋白的双重控制

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-23 DOI: 10.1016/j.devcel.2025.05.018

Ping Lu, Zhiyong Liu

引用次数: 0

Exploring the diversity of plant model organisms 探索植物模式生物的多样性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-23 DOI: 10.1016/j.devcel.2025.05.019

Andrew R.G. Plackett, John L. Bowman, Michael T. Raissig, Philippa Borrill, Sabeeha S. Merchant, Feng Qin, Enoch Lok Him Yuen, Tolga Bozkurt, Fang Xie

引用次数: 0

Transferrin receptor controls both autophagosome formation and closure via phosphatidylinositol 3-phosphate synthesis 转铁蛋白受体通过磷脂酰肌醇3-磷酸合成控制自噬体的形成和关闭

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-20 DOI: 10.1016/j.devcel.2025.05.016

Claudia Puri, So Jung Park, Lidia Wrobel, David C. Rubinsztein

{"title":"Transferrin receptor controls both autophagosome formation and closure via phosphatidylinositol 3-phosphate synthesis","authors":"Claudia Puri, So Jung Park, Lidia Wrobel, David C. Rubinsztein","doi":"10.1016/j.devcel.2025.05.016","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.016","url":null,"abstract":"Autophagosome formation involves multiple sequential steps that need to be coordinated and linked. Here, we describe in mammalian cells that the transferrin receptor (TfR) links LC3 family conjugation to phagophore membranes, an early step in autophagosome biogenesis, with subsequent autophagosome closure. TfR depletion impairs autophagic flux and its overexpression stimulates this catabolic process in an iron-independent manner. TfR is ubiquitinated by the ubiquitin ligase MARCH8 in the RAB11A—LC3B-positive membranes that are conjugated by LC3 family members from which phagophores emanate. Ubiquitinated TfR recruits the VPS34 component VPS15, enabling phosphatidylinositol 3-phosphate (PI(3)P) synthesis on nascent autophagosome membranes. This PI(3)P is not only important for LC3-lipid conjugation but also for subsequent phagophore closure, where TfR-dependent PI(3)P recruits the endosomal sorting complexes required for transport (ESCRT) complex. This TfR activity occurs after endocytosis of iron-containing transferrin, its canonical function, as TfR only binds VPS15 after iron detachment from transferrin that is enabled by pH lowering in the endocytic compartment.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"44 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TBX3 advances the developmental chromatin landscape toward the hepatic fate TBX3将染色质发育图景推向肝脏命运

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-18 DOI: 10.1016/j.devcel.2025.05.014

Tabea L. Stephan, Rebecca Cullum, Sibyl Drissler, Ashleigh Thomas, Bettina M. Fuglerud, Makenna Clement-Ranney, Jeremy Lotto, Nicole A.J. Krentz, Stephen A. Duncan, Pamela A. Hoodless

{"title":"TBX3 advances the developmental chromatin landscape toward the hepatic fate","authors":"Tabea L. Stephan, Rebecca Cullum, Sibyl Drissler, Ashleigh Thomas, Bettina M. Fuglerud, Makenna Clement-Ranney, Jeremy Lotto, Nicole A.J. Krentz, Stephen A. Duncan, Pamela A. Hoodless","doi":"10.1016/j.devcel.2025.05.014","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.014","url":null,"abstract":"By mapping histone modifications in a human stem cell model of hepatic differentiation, we identified an enhancer landscape that is dynamic and stage specific, with many primed at the definitive endoderm stage. While hepatic enhancers gained active histone modifications, non-hepatic enhancers lost H3K4me1 after hepatic specification. T-box transcription factor 3 (TBX3) was found to bind to hepatic enhancers and promoters. TBX3 binding was transient, and only upon the dissociation of TBX3 from its bound regions were active histone modifications acquired. Subsequently, transcription was activated, supporting a role for TBX3 in directly activating the hepatic lineage. Constitutive overexpression of TBX3 indicated that dissociation of TBX3 is crucial for gene activation. TBX3 did not bind to any pancreatic regulatory regions directly, indicating indirect repression of the pancreatic lineage. Together, this suggests that TBX3 plays a role in identifying and bookmarking hepatic enhancers and promoters during specification, which subsequently will be activated to drive cell identity.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"60 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular profiling of adult C. elegans glia across sexes by single-nuclear RNA-seq 用单核RNA-seq分析成年秀丽隐杆线虫胶质细胞的分子特征

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-16 DOI: 10.1016/j.devcel.2025.05.013

Maria D. Purice, Elgene J.A. Quitevis, R. Sean Manning, Liza J. Severs, Nina-Tuyen Tran, Violet Sorrentino, Connor Finkbeiner, Feinan Wu, Michael Zager, Manu Setty, Aakanksha Singhvi

{"title":"Molecular profiling of adult C. elegans glia across sexes by single-nuclear RNA-seq","authors":"Maria D. Purice, Elgene J.A. Quitevis, R. Sean Manning, Liza J. Severs, Nina-Tuyen Tran, Violet Sorrentino, Connor Finkbeiner, Feinan Wu, Michael Zager, Manu Setty, Aakanksha Singhvi","doi":"10.1016/j.devcel.2025.05.013","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.013","url":null,"abstract":"A comprehensive understanding of nervous system function requires molecular insight into the diversity and sex dimorphism of both its component cell types, glia and neurons. Here, we present a single-nuclear RNA sequencing (RNA-seq) census of all neuroectoderm-derived glia in the adult C. elegans nervous system, across sexes. By iteratively coupling computational modeling and custom analytics with in vivo validations, we uncovered molecular markers for all glia, as well as class-specific and pan-glial molecular signatures. These identified that each glia is functionally heterogeneous across the nervous system and variably sex dimorphic between sexes. Thus, this glial transcriptome (wormglia.org<svg aria-label=\"Opens in new window\" focusable=\"false\" height=\"20\" viewbox=\"0 0 8 8\"><path d=\"M1.12949 2.1072V1H7V6.85795H5.89111V2.90281L0.784057 8L0 7.21635L5.11902 2.1072H1.12949Z\"></path></svg>) offers deep mechanistic insights into glial biology brain wide. Complementing the existing C. elegans neuronal transcriptome and mapped connectome, it also enables single-cell and molecular resolution insight into the entire nervous system of an adult metazoan.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"43 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144296295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A transcriptional recognition site within SOS1 coding region controls salt tolerance in Arabidopsis SOS1编码区的转录识别位点控制拟南芥的耐盐性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-10 DOI: 10.1016/j.devcel.2025.05.010

Kai-Kai Lu, Hong Yang, Cai-Yi Liao, Ru-Feng Song, Xiao-Yu Hu, Feng Ren, Wen-Cheng Liu

{"title":"A transcriptional recognition site within SOS1 coding region controls salt tolerance in Arabidopsis","authors":"Kai-Kai Lu, Hong Yang, Cai-Yi Liao, Ru-Feng Song, Xiao-Yu Hu, Feng Ren, Wen-Cheng Liu","doi":"10.1016/j.devcel.2025.05.010","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.010","url":null,"abstract":"Salt stress impacts plant growth and development, threatening agricultural production. The Na+/H+ antiporter SALT OVERLY SENSITIVE 1 (SOS1) functions in cellular ion homeostasis through facilitating Na+ excretion and is therefore essential for plant salt tolerance. Here, we report that the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 are required for salt-induced SOS1 expression and salt tolerance in Arabidopsis thaliana. ARF7 and ARF19 activate SOS1 transcription by binding to SOS1 coding region rather than its promoter. Additionally, an E3 ubiquitin ligase, CHY ZINC-FINGER AND RING PROTEIN 1 (CHYR1), interacts with and degrades ARF7 and ARF19, dampening SOS1 expression. Upon high salinity, CHYR1 expression is inhibited in plants, stabilizing ARF7 and ARF19 proteins and increasing SOS1 expression. Collectively, our study identifies a transcriptional cis-element within SOS1 coding region recognized by ARF7 and ARF19 and elucidates a molecular mechanism governing ARF7 and ARF19 protein stability and SOS1 expression during plant salt stress response.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"90 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144252617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taking the “lazy” way identifies KCNB2 as a regulator of SHH-MB maintenance 采用“懒惰”的方法确定KCNB2是SHH-MB维持的调节因子

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-09 DOI: 10.1016/j.devcel.2025.04.016

Jaldeep Langhnoja, Timothy N. Phoenix

引用次数: 0

Condensates control the actin cytoskeleton 凝聚体控制肌动蛋白细胞骨架

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-09 DOI: 10.1016/j.devcel.2025.04.015

Xiaohang Cheng, Lindsay B. Case

引用次数: 0