Developmental cell最新文献_第10页

A chromatin reader connects salicylic acid signaling to the RNA polymerase II gearbox in plants. 在植物中，染色质解读器连接水杨酸信号和RNA聚合酶II变速箱。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.04.009

Eduardo Mateo-Bonmatí

引用次数: 0

Hematopoietic stem cells activate a latent differentiation pathway to facilitate recovery after 5-fluorouracil-induced myeloablation. 造血干细胞激活潜在分化途径，促进5-氟尿嘧啶诱导骨髓消融后的恢复。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 Epub Date: 2026-03-02 DOI: 10.1016/j.devcel.2026.02.003

Zhen Zhang, Ben Jin, Chenyu You, Ya Li, Li Lin, Jianlong Sun

{"title":"Hematopoietic stem cells activate a latent differentiation pathway to facilitate recovery after 5-fluorouracil-induced myeloablation.","authors":"Zhen Zhang, Ben Jin, Chenyu You, Ya Li, Li Lin, Jianlong Sun","doi":"10.1016/j.devcel.2026.02.003","DOIUrl":"10.1016/j.devcel.2026.02.003","url":null,"abstract":"Myeloablative chemotherapy induces hematopoietic regeneration, a process orchestrated by hematopoietic stem cells (HSCs). Although prior studies have documented enhanced HSC differentiation during this process, the temporal changes in HSC fate in response to such stress remain unclear. Here, we employed lineage tracing and mathematical modeling to investigate the cell-fate dynamics of Endothelial Protein C Receptor (EPCR)-high HSCs following 5-fluorouracil (5-FU)-induced myeloablation. Our analysis revealed a transient surge in HSC differentiation immediately after 5-FU treatment, generating primarily myeloid-biased multipotent progenitors (MPPs)-subsets that typically receive limited HSC input under steady-state conditions. Following this initial cell-fate switch, elevated HSC differentiation persisted but rapidly reverted to the homeostatic differentiation pattern observed in unperturbed hematopoiesis. Additionally, our data highlight a substantial contribution of MPPs to myeloid and lymphoid lineage regeneration following 5-FU challenge. Together, these findings delineate the sequential fate transitions adopted by HSCs during severe myeloablation and identify stage-specific differentiation patterns of HSCs in stress hematopoiesis.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1044-1060.e7"},"PeriodicalIF":9.2,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromatin-associated PP1 subcomplexes: Together, but not in each other's pockets. 染色质相关的PP1亚复合物：在一起，但不在彼此的口袋里。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.04.002

Sergio Moreno, Sandra Lopez-Aviles

引用次数: 0

CD155-CD96 keeps quiescent persister tumor cells in check. CD155-CD96控制静止的持久性肿瘤细胞。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.04.007

Johanna M Gorol, Florian R Greten

引用次数: 0

Multi-omic analyses reveal a differential contribution of chromatin-associated PP1 holoenzymes to mitotic exit and G1 re-establishment. 多组学分析揭示了染色质相关的PP1全酶对有丝分裂退出和G1重建的差异贡献。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 Epub Date: 2026-03-17 DOI: 10.1016/j.devcel.2026.02.016

Konstantinos Stamatiou, Florentin Huguet, Marta Budzinska, Jose I de Las Heras, Denise Ragusa, Ines J deCastro, Christos Spanos, Juri Rappsilberg, Paola Vagnarelli

{"title":"Multi-omic analyses reveal a differential contribution of chromatin-associated PP1 holoenzymes to mitotic exit and G1 re-establishment.","authors":"Konstantinos Stamatiou, Florentin Huguet, Marta Budzinska, Jose I de Las Heras, Denise Ragusa, Ines J deCastro, Christos Spanos, Juri Rappsilberg, Paola Vagnarelli","doi":"10.1016/j.devcel.2026.02.016","DOIUrl":"10.1016/j.devcel.2026.02.016","url":null,"abstract":"Mitotic exit is an important part of the cell cycle, requiring the coordination of many chromatin and cytoskeleton remodeling events to successfully complete cell division and maintain cell identity. Protein dephosphorylation is a key step in directing mitotic exit, and protein phosphatase 1 (PP1) is essential to this process; however, the specific contribution of its numerous targeting subunits is still unknown. Here, we have investigated the function of three chromatin-associated PP1-targeting subunits in mitosis exit: Repo-Man, Ki-67, and protein phosphatase 1 nuclear targeting subunit (PNUTS). We generated endogenously tagged, auxin-degradable alleles for each subunit in the human cell line HCT116 and used a multi-omic approach to address their specific contributions toward transcription resumption, chromatin accessibility, and protein dephosphorylation at the transition from mitosis to G1. This approach identified their distinct role in mitotic exit, provided datasets for the cell-cycle community, and highlighted functions for Ki-67 and Repo-Man in genome stability and organization.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1127-1145.e8"},"PeriodicalIF":9.2,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147480052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additional layers of systemic plant immunity: Apoplastic pH and uORF peptides. 系统植物免疫的附加层：胞外pH和uORF肽。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.04.008

Hannah Weber, Birgit Kemmerling

引用次数: 0

The want for Wnt: Pancreatic cancer assembles a self-sustaining Wnt-signaling ecosystem. 对Wnt的需求：胰腺癌组装了一个自我维持的Wnt信号生态系统。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.04.010

Nikki K Lytle, Laura D Wood

引用次数: 0

Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in vivo. 单分子线粒体DNA成像揭示了体内发育瓶颈和选择形成的异质性动力学。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-05-13 DOI: 10.1016/j.devcel.2026.03.011

Rajini Chandrasegaram, Sara Gottardo, Abhilesh Dhawanjewar, Antony M Hynes-Allen, Beitong Gao, Suvagata Roy Chowdhury, Luis-Carlos Tábara, Michele Frison, Stavroula Petridi, Patrick F Chinnery, Julien Prudent, Hansong Ma, Jelle van den Ameele

{"title":"Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in vivo.","authors":"Rajini Chandrasegaram, Sara Gottardo, Abhilesh Dhawanjewar, Antony M Hynes-Allen, Beitong Gao, Suvagata Roy Chowdhury, Luis-Carlos Tábara, Michele Frison, Stavroula Petridi, Patrick F Chinnery, Julien Prudent, Hansong Ma, Jelle van den Ameele","doi":"10.1016/j.devcel.2026.03.011","DOIUrl":"10.1016/j.devcel.2026.03.011","url":null,"abstract":"Mitochondrial DNA (mtDNA) exists in many copies per cell, with cell-to-cell variability in mutation load, which is known as heteroplasmy. Developmental and age-related expansion of heteroplasmic mtDNA mutations contributes to the pathogenesis of mitochondrial and neurodegenerative diseases. Here, we describe an approach for in situ sequence-specific detection of single mtDNA molecules (mtDNA-single-molecule fluorescent in situ hybridization [smFISH]). We apply this method to visualize and measure mtDNA and heteroplasmy levels in situ at single-cell resolution in whole-mount Drosophila tissue and cultured human cells. In Drosophila, we identify a somatic mtDNA bottleneck during neurogenesis. This amplifies heteroplasmy variability between neurons, as predicted by a mathematical bottleneck model, predisposing individual neurons to a high mutation load. However, both during neurogenesis and oogenesis, mtDNA segregation is accompanied by purifying selection, promoting wild-type (WT) over pathogenic mtDNA. mtDNA-smFISH thus elucidates how developmental cell-fate transitions, accompanied by changes in cell morphology, behavior, and metabolism, can shape the transmission and selection of deleterious mtDNA variants.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"61 5","pages":"1146-1161.e8"},"PeriodicalIF":9.2,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7619573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147927399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy 骨骼肌图谱显示神经肌肉连接适应生长和萎缩

IF 11.8 1区生物学

Developmental cell Pub Date : 2026-04-21 DOI: 10.1016/j.devcel.2026.03.010

Silvia Campanario, Mercedes Grima-Terrén, Megan Rommelfanger, Stefania Dell’ Orso, Xuesong Feng, Andrés Cisneros, Ignacio Ramírez-Pardo, Aina Calls-Cobos, Benjamin A. Yang, Kyung Dae Ko, Esther García-Domínguez, Laura Pena-Couso, Grace Chou, Yuewen Zheng, Nasun Hah, Davide Randazzo, Alberto Pérez-Garcia, Tovah E. Markowitz, Jose Milisenda, Iago Pinal-Fernandez, Pura Muñoz-Cánoves

{"title":"A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy","authors":"Silvia Campanario, Mercedes Grima-Terrén, Megan Rommelfanger, Stefania Dell’ Orso, Xuesong Feng, Andrés Cisneros, Ignacio Ramírez-Pardo, Aina Calls-Cobos, Benjamin A. Yang, Kyung Dae Ko, Esther García-Domínguez, Laura Pena-Couso, Grace Chou, Yuewen Zheng, Nasun Hah, Davide Randazzo, Alberto Pérez-Garcia, Tovah E. Markowitz, Jose Milisenda, Iago Pinal-Fernandez, Pura Muñoz-Cánoves","doi":"10.1016/j.devcel.2026.03.010","DOIUrl":"https://doi.org/10.1016/j.devcel.2026.03.010","url":null,"abstract":"The molecular basis underlying muscle atrophy, as it occurs during disuse or aging, and activity-induced hypertrophy remain poorly understood. A major challenge has been defining the diverse cellular and niche environments within skeletal muscle, which is mostly composed of multinucleated myofibers. Here, we present a single-nucleus and single-cell transcriptomic atlas, coupled with spatial profiling, of mouse limb skeletal muscle under resting conditions and during experimentally induced atrophy or hypertrophy. We identify condition-dependent shifts in muscle-resident cell populations and fiber-type-specific transcriptional responses. We also uncover extensive remodeling of the neuromuscular junction (NMJ), including the emergence of specialized synaptic myonuclei (SynM) and terminal Schwann cells (tSCs) associated with atrophic or hypertrophic states. High-resolution 3D imaging and spatial transcriptomics confirm these changes at the tissue level. Similar NMJ alterations are observed in denervated and exercised human muscle, supporting the translational relevance of this atlas for studying muscle plasticity and identifying therapeutic targets in muscle-related diseases.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"136 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147726414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AMBRA1 enhances Sonic Hedgehog signaling during cerebellar development and in medulloblastoma AMBRA1在小脑发育和成神经管细胞瘤中增强Sonic Hedgehog信号

IF 11.8 1区生物学

Developmental cell Pub Date : 2026-04-13 DOI: 10.1016/j.devcel.2026.03.008

Irene Basili, Francesca Bufalieri, Marilisa Conenna, Shirin Navacci, Yue-Ru Li, Jacob Torrejon, Flavia Bernardi, Ludovica Lospinoso Severini, Veronique Marsaud, Gabriele Cancila, Guillaume Bourmeau, Hua Yu, Valentina Lo Re, Julie Talbot, Gennaro Adabbo, Alberico Di Pinto, Francesca Agnoli, Daniela Giovannini, Simona Leonardi, Rosa Bordone, Lucia Di Marcotullio

{"title":"AMBRA1 enhances Sonic Hedgehog signaling during cerebellar development and in medulloblastoma","authors":"Irene Basili, Francesca Bufalieri, Marilisa Conenna, Shirin Navacci, Yue-Ru Li, Jacob Torrejon, Flavia Bernardi, Ludovica Lospinoso Severini, Veronique Marsaud, Gabriele Cancila, Guillaume Bourmeau, Hua Yu, Valentina Lo Re, Julie Talbot, Gennaro Adabbo, Alberico Di Pinto, Francesca Agnoli, Daniela Giovannini, Simona Leonardi, Rosa Bordone, Lucia Di Marcotullio","doi":"10.1016/j.devcel.2026.03.008","DOIUrl":"https://doi.org/10.1016/j.devcel.2026.03.008","url":null,"abstract":"AMBRA1 (autophagy and beclin 1 regulator 1) is primarily recognized as a tumor suppressor. However, its role as a tumor promoter has garnered increasing attention. Here, leveraging clinical data of an international multi-omic medulloblastoma (MB) cohort, we identified that elevated AMBRA1 protein levels, independently of its mRNA expression, correlate with poor prognosis in the Sonic Hedgehog subgroup (MBSHH). Mechanistically, AMBRA1 enhances SHH signaling by stabilizing GLI1, the pathway’s final effector, via inhibition of its βTrCP-mediated degradation. Additionally, AMBRA1 protein stability is modulated by the REN E3 ubiquitin ligase, a tumor suppressor gene lost in MBSHH. Inhibition of AMBRA1 blocks MBSHH growth in murine and patient-derived preclinical models. Moreover, combining AMBRA1 knockdown with FDA-approved SHH inhibitors enhances antitumor efficacy. These findings identify the AMBRA1/βTrCP/REN axis as a key regulatory mechanism in SHH signaling and discover an unrecognized function of AMBRA1 in MBSHH, providing actionable insights into brain tumor biology.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"5 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147666430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0