Developmental cell最新文献_第9页

Tuft cells transdifferentiate to neural-like progenitor cells in the progression of pancreatic cancer 在胰腺癌的发展过程中，簇状细胞向神经样祖细胞转化

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-24 DOI: 10.1016/j.devcel.2024.12.003

Daniel J. Salas-Escabillas, Megan T. Hoffman, Sydney M. Brender, Jacee S. Moore, Hui-Ju Wen, Simone Benitz, Erick T. Davis, Daniel Long, Allison M. Wombwell, Ella Rose D. Chianis, Brittany L. Allen-Petersen, Nina G. Steele, Rosalie C. Sears, Ichiro Matsumoto, Kathleen E. DelGiorno, Howard C. Crawford

{"title":"Tuft cells transdifferentiate to neural-like progenitor cells in the progression of pancreatic cancer","authors":"Daniel J. Salas-Escabillas, Megan T. Hoffman, Sydney M. Brender, Jacee S. Moore, Hui-Ju Wen, Simone Benitz, Erick T. Davis, Daniel Long, Allison M. Wombwell, Ella Rose D. Chianis, Brittany L. Allen-Petersen, Nina G. Steele, Rosalie C. Sears, Ichiro Matsumoto, Kathleen E. DelGiorno, Howard C. Crawford","doi":"10.1016/j.devcel.2024.12.003","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.12.003","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDA) is partly initiated through the transdifferentiation of acinar cells to metaplasia, which progresses to neoplasia and cancer. Tuft cells (TCs) are chemosensory cells not found in the normal pancreas but arise in cancer precursor lesions and diminish during progression to carcinoma. These metaplastic TCs (mTCs) suppress tumor progression through communication with the tumor microenvironment, but their fate during progression is unknown. To determine the fate of mTCs during PDA progression, we created a dual recombinase lineage trace model, wherein a pancreas-specific FlpO was used to induce tumorigenesis, while a tuft-cell specific Pou2f3CreERT/+ driver was used to induce expression of a tdTomato reporter. We found that mTCs in carcinoma transdifferentiate into neural-like progenitor cells (NRPs), a cell type associated with poor survival in patients. Using conditional knockout and overexpression systems, we found that Myc activity in mTCs is necessary and sufficient to induce this tuft-to-neuroendocrine transition (TNT).","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"20 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography 用冷冻电子断层扫描观察秀丽隐杆线虫胚胎中心体的分子结构

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-24 DOI: 10.1016/j.devcel.2024.12.002

Fergus Tollervey, Manolo U. Rios, Evgenia Zagoriy, Jeffrey B. Woodruff, Julia Mahamid

{"title":"Molecular architectures of centrosomes in C. elegans embryos visualized by cryo-electron tomography","authors":"Fergus Tollervey, Manolo U. Rios, Evgenia Zagoriy, Jeffrey B. Woodruff, Julia Mahamid","doi":"10.1016/j.devcel.2024.12.002","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.12.002","url":null,"abstract":"Centrosomes organize microtubules that are essential for mitotic divisions in animal cells. They consist of centrioles surrounded by pericentriolar material (PCM). Questions related to mechanisms of centriole assembly, PCM organization, and spindle microtubule formation remain unanswered, partly due to limited availability of molecular-resolution structural data inside cells. Here, we use cryo-electron tomography to visualize centrosomes across the cell cycle in cells isolated from C. elegans embryos. We describe a pseudo-timeline of centriole assembly and identify distinct structural features in both mother and daughter centrioles. We find that centrioles and PCM microtubules differ in protofilament number (13 versus 11), which could be explained by atypical γ-tubulin ring complexes with 11-fold symmetry identified at the minus ends of short PCM microtubule segments. We further characterize a porous and disordered network that forms the interconnected PCM. Thus, our work builds a three-dimensional structural atlas that helps explain how centrosomes assemble, grow, and achieve function.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"24 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global versus local matrix remodeling drives rotational versus invasive collective migration of epithelial cells 整体与局部基质重塑驱动上皮细胞旋转与侵袭性集体迁移

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-19 DOI: 10.1016/j.devcel.2024.11.021

Sural K. Ranamukhaarachchi, Alyssa Walker, Man-Ho Tang, William D. Leineweber, Sophia Lam, Wouter-Jan Rappel, Stephanie I. Fraley

{"title":"Global versus local matrix remodeling drives rotational versus invasive collective migration of epithelial cells","authors":"Sural K. Ranamukhaarachchi, Alyssa Walker, Man-Ho Tang, William D. Leineweber, Sophia Lam, Wouter-Jan Rappel, Stephanie I. Fraley","doi":"10.1016/j.devcel.2024.11.021","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.11.021","url":null,"abstract":"The coordinated movement of cell collectives is essential for normal epithelial tissue development, maintenance, and cancer progression. Here, we report on a minimal 3D extracellular matrix (ECM) system wherein both invasive collective migration (ICM) and rotational collective migration (RCM) arise spontaneously from individually seeded epithelial cells of mammary and hepatic origin, regardless of whether they express adherens junctions, and lead to ductal-like and acinar-like structures, respectively. Quantitative microscopy and cellular Potts modeling reveal that initial differences in cell protrusion dynamics and matrix-remodeling localization generate RCM and ICM behavior in confining 3D ECM. Matrix-remodeling activity by matrix metalloproteinases (MMPs) is localized to the base of protrusions in cells that initiate ICM, whereas RCM does not require MMPs and is associated with ITGβ1-mediated remodeling localized globally around the cell body. Further analysis in vitro and in vivo supports the concept that distinct matrix-remodeling strategies encode collective migration behaviors and tissue structure.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"113 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142849565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plant signal peptide CLE7 induces plant defense response against viral infection in Nicotiana benthamiana 植物信号肽CLE7诱导植物防御病毒感染

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-16 DOI: 10.1016/j.devcel.2024.11.020

Peng Liu, Juan Zhang, Shuang Liu, Yaoyao Li, Chunyan Qi, Qitao Mo, Yaoyao Jiang, Haichao Hu, Tianye Zhang, Kaili Zhong, Jianqian Liu, Qiansheng Liao, Jianping Chen, Jian Yang

{"title":"The plant signal peptide CLE7 induces plant defense response against viral infection in Nicotiana benthamiana","authors":"Peng Liu, Juan Zhang, Shuang Liu, Yaoyao Li, Chunyan Qi, Qitao Mo, Yaoyao Jiang, Haichao Hu, Tianye Zhang, Kaili Zhong, Jianqian Liu, Qiansheng Liao, Jianping Chen, Jian Yang","doi":"10.1016/j.devcel.2024.11.020","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.11.020","url":null,"abstract":"In plants, small peptides are important players in the plant stress response, yet their function in plant antiviral responses remains poorly understood. Here, we identify that the plant small peptide, CLAVATA3/ESR-RELATED 7 (CLE7), enhances plant resistance to Chinese wheat mosaic virus infection in Nicotiana (N.) benthamiana. Subsequent investigations demonstrate that CLE7 recognizes receptor kinase NbPXC3 to control the plant antiviral response. Moreover, CLE7-NbPXC3 signaling induces NbMKK2-controlled NbMPK4 phosphorylation, resulting in phosphorylation of the transcription factor NbEDT1. NbEDT1 phosphorylation is involved in the transcriptional activity of NbNCED3, which is a rate-limiting enzyme in abscisic acid (ABA) biosynthesis. Moreover, CLE7 activates broad-spectrum disease resistance to multiple RNA viral infections. Our study indicates that CLE7 induces a plant antiviral response through a series of immune signal transductions in N. benthamiana and provides a foundation for the exploration of efficient viral disease management methods based on plant small peptides.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"28 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoplasmic reticulum-anchored proteins in control of AUXIN RESPONSE FACTOR condensation 内质网锚定蛋白控制生长素反应因子缩聚

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-16 DOI: 10.1016/j.devcel.2024.11.004

Cédric Schindfessel, Steffen Vanneste

引用次数: 0

Emergence of the cell-matrix adhesion element ILK as a player in glioblastoma cell plasticity 细胞基质粘附因子ILK在胶质母细胞瘤细胞可塑性中的作用

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-16 DOI: 10.1016/j.devcel.2024.11.012

Marie-Pierre Junier, Elias A. El-Habr

引用次数: 0

Axon guidance in central nervous system regeneration 中枢神经系统再生中的轴突引导

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-16 DOI: 10.1016/j.devcel.2024.11.013

Brent K. Young, Jeffrey L. Goldberg

引用次数: 0

Apical hook opening of plant seedlings: Unfolding the role of auxin and the cell wall 植物幼苗顶钩开口：揭示生长素与细胞壁的作用

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-16 DOI: 10.1016/j.devcel.2024.11.018

Andrew C. Willoughby, Lucia C. Strader

引用次数: 0

An amino acid-resolution interactome for motile cilia identifies the structure and function of ciliopathy protein complexes 运动纤毛的氨基酸分辨率相互作用组识别纤毛病蛋白复合物的结构和功能

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-13 DOI: 10.1016/j.devcel.2024.11.019

Caitlyn L. McCafferty, Ophelia Papoulas, Chanjae Lee, Khanh Huy Bui, David W. Taylor, Edward M. Marcotte, John B. Wallingford

{"title":"An amino acid-resolution interactome for motile cilia identifies the structure and function of ciliopathy protein complexes","authors":"Caitlyn L. McCafferty, Ophelia Papoulas, Chanjae Lee, Khanh Huy Bui, David W. Taylor, Edward M. Marcotte, John B. Wallingford","doi":"10.1016/j.devcel.2024.11.019","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.11.019","url":null,"abstract":"Motile cilia are ancient, evolutionarily conserved organelles whose dysfunction underlies motile ciliopathies, a broad class of human diseases. Motile cilia contain a myriad of different proteins that assemble into an array of distinct machines, and understanding the interactions and functional hierarchies among them presents an important challenge. Here, we defined the protein interactome of motile axonemes using cross-linking mass spectrometry in Tetrahymena thermophila. From over 19,000 cross-links, we identified over 4,700 unique amino acid interactions among over 1,100 distinct proteins, providing both macromolecular and atomic-scale insights into diverse ciliary machines, including the intraflagellar transport system, axonemal dynein arms, radial spokes, the 96-nm ruler, and microtubule inner proteins. Guided by this dataset, we used vertebrate multiciliated cells to reveal functional interactions among several poorly defined human ciliopathy proteins. This dataset provides a resource for studying the biology of an ancient organelle and the molecular etiology of human genetic disease.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"8 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collective oscillatory signaling in Dictyostelium discoideum acts as a developmental timer initiated by weak coupling of a noisy pulsatile signal 盘状盘齿柱的集体振荡信号作为一个发育定时器，由一个有噪声的脉冲信号的弱耦合启动

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-12-12 DOI: 10.1016/j.devcel.2024.11.016

Christopher A. Brimson, Robert Baines, Elisabeth Sams-Dodd, Ioanina Stefanescu, Bethany Evans, Satoshi Kuwana, Hidenori Hashimura, Satoshi Sawai, Christopher R.L. Thompson

{"title":"Collective oscillatory signaling in Dictyostelium discoideum acts as a developmental timer initiated by weak coupling of a noisy pulsatile signal","authors":"Christopher A. Brimson, Robert Baines, Elisabeth Sams-Dodd, Ioanina Stefanescu, Bethany Evans, Satoshi Kuwana, Hidenori Hashimura, Satoshi Sawai, Christopher R.L. Thompson","doi":"10.1016/j.devcel.2024.11.016","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.11.016","url":null,"abstract":"Oscillatory phenomena play widespread roles in the control of biological systems. In D. discoideum, oscillatory cyclic adenosine monophosphate (cAMP) signaling drives collective behavior and induces a temporal developmental gene expression program. How collective cAMP oscillations emerge or how they encode temporal transcriptional information is still poorly understood. To address this, we identified a transcription factor required for the initiation of collective behavior. Hbx5 activity is cAMP dependent and provides a sensitive single-cell readout for cAMP signaling. Extensive stochastic pulsatile cAMP signaling is found to precede collective oscillations. Stochastic signaling induces Hbx5-dependent transcriptional feedback, which enhances signal sensitivity and cell-cell coupling. This results in the emergence of synchronized collective oscillations, which subsequently activates the GtaC transcription factor and triggers shifts in developmental gene expression. Our results suggest this temporal coordination is encoded by changes in the amplitude of cAMP oscillations and differential sensitivity of these transcription factors to the cAMP-regulated kinase ErkB.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"1 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142809461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0