Developmental cell最新文献_第8页

Right on cue-Neuronal signals guide myelination in the developing cortex. 右图：神经元信号引导皮层发育中的髓鞘形成。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 DOI: 10.1016/j.devcel.2026.05.009

Albert Zhang, Jonah R Chan

引用次数: 0

Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformation. 人类胰腺祖类器官定义了早期PDAC转化的遗传和表观遗传障碍。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-05-19 DOI: 10.1016/j.devcel.2026.04.012

Xian Zhang, Wilfred Wong, Hyein S Cho, Dapeng Yang, Gary Dixon, Renhe Luo, Dingyu Liu, Denis Torre, Shigeaki Umeda, Federico González, Gokce Askan, Aslihan Yavas, Jeyaram Ravichandran Damodaran, Robert W Rickert, Rajya Kappagantula, Fong Cheng Pan, Victoria G Aveson, Adrien Grimont, William B Fall, Xianming Wang, Julian Pulecio, Samuel J Kaplan, Heng Pan, Xianlu Laura Peng, Jen Jen Yeh, Steven D Leach, Christine A Iacobuzio-Donahue, Rohit Chandwani, Christina S Leslie, Danwei Huangfu

{"title":"Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformation.","authors":"Xian Zhang, Wilfred Wong, Hyein S Cho, Dapeng Yang, Gary Dixon, Renhe Luo, Dingyu Liu, Denis Torre, Shigeaki Umeda, Federico González, Gokce Askan, Aslihan Yavas, Jeyaram Ravichandran Damodaran, Robert W Rickert, Rajya Kappagantula, Fong Cheng Pan, Victoria G Aveson, Adrien Grimont, William B Fall, Xianming Wang, Julian Pulecio, Samuel J Kaplan, Heng Pan, Xianlu Laura Peng, Jen Jen Yeh, Steven D Leach, Christine A Iacobuzio-Donahue, Rohit Chandwani, Christina S Leslie, Danwei Huangfu","doi":"10.1016/j.devcel.2026.04.012","DOIUrl":"10.1016/j.devcel.2026.04.012","url":null,"abstract":"The lack of accurate human models that recapitulate pancreatic ductal adenocarcinoma (PDAC) initiation has hindered therapeutic development. Using pluripotent stem cell-derived pancreatic progenitor organoids, we established a human PDAC model that faithfully reproduces the genetic, epigenetic, and transcriptomic trajectories of tumor initiation and progression, validated against clinical datasets and tumor histopathology. We demonstrate that CDKN2A loss, which is nearly universal in patients but dispensable in mouse models, is essential for neoplastic transformation when combined with KRAS and TP53 mutations, whereas SMAD4 loss promotes tumor progression. Multi-omics profiling reveals epigenetic repression of the pancreatic lineage program during PDAC initiation, alongside AP-1-driven chromatin remodeling. We identify TET1 suppression as a mechanistic link between oncogenic ERK signaling and hypermethylation of essential pancreatic transcription factors. This model captures genetic and epigenetic determinants of human PDAC, reveals antagonism between oncogenic and lineage restriction programs, and supports TET-based lineage restoration as a potential early intervention strategy.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1190-1207.e12"},"PeriodicalIF":9.2,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13196429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion-controlled mechanics of the glial niche regulate neural stem cell proliferative potential. 胶质小生境的粘附控制机制调节神经干细胞的增殖潜能。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-05-18 DOI: 10.1016/j.devcel.2026.04.011

Anna Segú Cristina, Gaëlle Letort, Emeline Perthame, Stéphane Rigaud, David Briand, Aman Kukde, Agata Banach-Latapy, Nick D L Owens, Léo Valon, Jean-Yves Tinevez, Yohanns Bellaïche, Pauline Spéder

{"title":"Adhesion-controlled mechanics of the glial niche regulate neural stem cell proliferative potential.","authors":"Anna Segú Cristina, Gaëlle Letort, Emeline Perthame, Stéphane Rigaud, David Briand, Aman Kukde, Agata Banach-Latapy, Nick D L Owens, Léo Valon, Jean-Yves Tinevez, Yohanns Bellaïche, Pauline Spéder","doi":"10.1016/j.devcel.2026.04.011","DOIUrl":"10.1016/j.devcel.2026.04.011","url":null,"abstract":"Stem cell proliferation drives tissue formation and homeostasis. Whether and how niche cell mechanics act in vivo to control this process remains unclear. Here, we identify a triad of cell adhesion molecules from the immunoglobulin superfamily, which physically link neural stem cells (NSCs) to their glial niche in Drosophila. Disrupting this connection increases actomyosin activation and tensile forces in the glial cells, reciprocally causing mechanical stress in NSCs. This promotes lamin accumulation, which causes nuclear deformation and acts as a protective response safeguarding NSC mitosis. However, this response is insufficient, and NSCs display abnormal spindle morphologies and impaired mitotic progression. Ultimately, the loss of NSC and niche mechanical interplay compromises NSC proliferative potential and genome integrity. This study uncovers a fundamental role for niche cell mechanics and members of the immunoglobulin superfamily, acting as mechanoregulators, in controlling stem cell proliferation.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1254-1272.e11"},"PeriodicalIF":8.7,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147971339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dec2 conceals tumors from the immune system. Dec2对免疫系统隐藏肿瘤。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 DOI: 10.1016/j.devcel.2026.05.007

Tianyue Sun, Christoph Scheiermann

引用次数: 0

Sensing forces: How plants convert mechanical cues into biological responses. 感应力：植物如何将机械信号转化为生物反应。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 DOI: 10.1016/j.devcel.2026.05.003

Pauline Vinet, Jean-Marie Frachisse, Sébastien Thomine, Pauline Durand-Smet

引用次数: 0

A machine learning-based megakaryocyte identification system uncovers resident organs, markers, and functional diversity. 基于机器学习的巨核细胞识别系统揭示了常驻器官，标记物和功能多样性。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-05-11 DOI: 10.1016/j.devcel.2026.04.005

Meijuan Xia, Yezi Ma, Yifei Cai, Jingjing Zhao, Yao Zhong, Sibei Guo, Minmin Li, Pei Su, Biao Shen, Huizhen He, Xiaoyuan Chen, Lin Zheng, Le Li, Ziqi Huo, Wen Zhou, Fei Wang, Cuicui Liu, Hongtao Wang, Jiaxi Zhou

{"title":"A machine learning-based megakaryocyte identification system uncovers resident organs, markers, and functional diversity.","authors":"Meijuan Xia, Yezi Ma, Yifei Cai, Jingjing Zhao, Yao Zhong, Sibei Guo, Minmin Li, Pei Su, Biao Shen, Huizhen He, Xiaoyuan Chen, Lin Zheng, Le Li, Ziqi Huo, Wen Zhou, Fei Wang, Cuicui Liu, Hongtao Wang, Jiaxi Zhou","doi":"10.1016/j.devcel.2026.04.005","DOIUrl":"10.1016/j.devcel.2026.04.005","url":null,"abstract":"The organ- and stage-specific diversity of megakaryocytes (MKs) has prompted a reassessment of their distribution and functions. By integrating single-cell transcriptomic data across multiple organs and developmental stages, we identified previously unreported MK and platelet markers, including Tnik, a key regulator of MK function and platelet production. Using these markers alongside established ones, we developed a machine learning-based MK identification system (MKIDS) that enables MK detection in the brain, heart, and placenta in mice and humans. Functional studies demonstrated that brain-resident MKs are essential for neural development, underscoring organ-specific roles of MKs in regulating tissue development and function. Transcriptomic integration of MKs across organs and stages, with functional validation, revealed a developmental shift in platelet production-from a mitochondria-low to a mitochondria-enriched subpopulation. Our findings offer a transformative perspective on the MK system, highlighting its cellular diversity, functional complexity, and developmental dynamics.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1319-1339.e8"},"PeriodicalIF":8.7,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147927319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic regulation of cancer cell stiffness and metastatic colonization via the MRTFA-KCNMB1 axis. 通过MRTFA-KCNMB1轴对癌细胞硬度和转移定植的离子调节。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-06-02 DOI: 10.1016/j.devcel.2026.05.001

Alexa M Gajda, Mohamed Haloul, Vinay Pai, Keyvan Mollaeian, Khushi J Patel, Raymundo Rodríguez-López, Katie M Beverley, Mark A Sanborn, Kihak Lee, Caitlyn C Castillo, Stephanie M Wilk, Beata M Wolska, Faruk Hossen, Eron N Mendenhall, James C Lee, Irena Levitan, Jalees Rehman, Ekrem Emrah Er

{"title":"Ionic regulation of cancer cell stiffness and metastatic colonization via the MRTFA-KCNMB1 axis.","authors":"Alexa M Gajda, Mohamed Haloul, Vinay Pai, Keyvan Mollaeian, Khushi J Patel, Raymundo Rodríguez-López, Katie M Beverley, Mark A Sanborn, Kihak Lee, Caitlyn C Castillo, Stephanie M Wilk, Beata M Wolska, Faruk Hossen, Eron N Mendenhall, James C Lee, Irena Levitan, Jalees Rehman, Ekrem Emrah Er","doi":"10.1016/j.devcel.2026.05.001","DOIUrl":"10.1016/j.devcel.2026.05.001","url":null,"abstract":"Cellular stiffness impacts multiple steps of cancer metastasis, but mechanisms that regulate the stiffness of cancer cells remain poorly understood. Here, we identified potassium efflux and potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), an auxiliary subunit of the large conductance calcium-activated potassium (BK) channels, as regulators of cellular stiffness downstream of myocardin-related transcription factor A (MRTFA). In primary pericytes, KCNMB1 knockdown increased cellular stiffness, which is consistent with the role of potassium efflux in promoting relaxation during excitation-contraction coupling. In a striking contrast, however, KCNMB1 knockdown decreased cancer cells' stiffness. Softer cancer cells were resistant to natural killer (NK) cell mediated cytotoxicity and the low KCNMB1 expression was associated with reduced survival in breast cancer patients. Importantly, pharmacological activation of BK channels reduced metastatic burden in mice and improved lysis of cancer cells by cytotoxic T lymphocytes. These results highlight the ionic regulation of stiffness in cancer cells and point to BK channel agonism as a therapeutic approach.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1223-1237.e7"},"PeriodicalIF":9.2,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A human model of progressive pancreatic neoplasia and cancer. 进展性胰腺肿瘤和癌症的人类模型。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 DOI: 10.1016/j.devcel.2026.05.005

David Escobar, Howard C Crawford

引用次数: 0

Spatiotemporal mapping of the contractile and adhesive forces sculpting early C. elegans embryos. 早期秀丽隐杆线虫胚胎的收缩力和粘附力的时空映射。

IF 8.7 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-05-21 DOI: 10.1016/j.devcel.2026.04.013

Kazunori Yamamoto, Sacha Ichbiah, Matthieu Perez, Joana Borrego-Pinto, Fabrice Delbary, Nathan W Goehring, Paul Northrop, Hervé Turlier, Guillaume Charras

{"title":"Spatiotemporal mapping of the contractile and adhesive forces sculpting early C. elegans embryos.","authors":"Kazunori Yamamoto, Sacha Ichbiah, Matthieu Perez, Joana Borrego-Pinto, Fabrice Delbary, Nathan W Goehring, Paul Northrop, Hervé Turlier, Guillaume Charras","doi":"10.1016/j.devcel.2026.04.013","DOIUrl":"10.1016/j.devcel.2026.04.013","url":null,"abstract":"Embryo shape is determined by cell mechanics, intercellular interactions, and geometrical constraints. Models based on surface tensions at cell interfaces can predict 3D static cellular arrangements within aggregates. However, predicting the dynamics of such arrangements is challenging because temporal changes in tension are unknown. Here, we characterize the spatiotemporal changes in cellular tensions shaping early nematode embryos using atomic force microscopy (AFM), live microscopy, and tension inference. Using excoriated embryos, we validate a hybrid inference pipeline that temporally calibrates relative inferred tensions using cortical myosin enrichment and absolute tensions obtained by AFM. Applying this approach to embryos within their native shell provides a spatiotemporal map of absolute tensions. This reveals that ABa, ABp, and EMS compaction is driven by increased cell-medium tension, whereas P2's initial exclusion is due to high cell-cell contact tension. We further uncover a direct contribution of cadherins to cell-cell contact tension, comparable in magnitude to cadherins' indirect contribution via actomyosin regulation.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1273-1288.e4"},"PeriodicalIF":8.7,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147986945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The circadian gene Dec2 promotes pancreatic cancer progression and dormancy through immune evasion. 昼夜节律基因Dec2通过免疫逃避促进胰腺癌的进展和休眠。

IF 9.2 1区生物学

Developmental cell Pub Date : 2026-06-10 Epub Date: 2026-04-28 DOI: 10.1016/j.devcel.2026.04.001

Lan Wang, Chris R Harris, Crissy Dudgeon, Orjola Prela, Juliana Cazarin, Ching-Hua Shih, Christina Davidson, Anthony Casabianca, Subhajyoti De, Wade Narrow, Jennifer Becker, Paul M Grandgenett, Michael A Hollingsworth, Jean L Grem, Minsoo Kim, Yeonsun Hong, Scott Gerber, Paula M Vertino, Chongfeng Gao, Zachary Klamer, Anna Repesh, Yansheng Hao, Allison T Ryan, Mitchell Breitenbach, Anna Bianchi, Jashodeep Datta, Brian J Altman, Brian Haab, Darren R Carpizo

{"title":"The circadian gene Dec2 promotes pancreatic cancer progression and dormancy through immune evasion.","authors":"Lan Wang, Chris R Harris, Crissy Dudgeon, Orjola Prela, Juliana Cazarin, Ching-Hua Shih, Christina Davidson, Anthony Casabianca, Subhajyoti De, Wade Narrow, Jennifer Becker, Paul M Grandgenett, Michael A Hollingsworth, Jean L Grem, Minsoo Kim, Yeonsun Hong, Scott Gerber, Paula M Vertino, Chongfeng Gao, Zachary Klamer, Anna Repesh, Yansheng Hao, Allison T Ryan, Mitchell Breitenbach, Anna Bianchi, Jashodeep Datta, Brian J Altman, Brian Haab, Darren R Carpizo","doi":"10.1016/j.devcel.2026.04.001","DOIUrl":"10.1016/j.devcel.2026.04.001","url":null,"abstract":"The mechanisms that regulate immune evasion by pancreatic ductal adenocarcinomas (PDACs) remain poorly understood. Using a mouse model of resectable PDAC, we identified an unknown role of the circadian rhythm gene Differentially Expressed in Chondrocytes 2 (Dec2) in regulating tumor progression and dormancy. Deletion of Dec2 from tumor cells substantially increased mouse survival after resection due to an immune-mediated mechanism, as the survival benefit was abrogated under immunodeficient conditions. Dec2 promotes immune evasion by repressing major histocompatibility complex class I (MHC-I)-dependent antigen presentation and by repolarizing the tumor microenvironment from immunologically cold (low T cell infiltration) to hot (elevated T cell infiltration). Dec2 is also a regulator of circadian rhythms, and we found that genes involved in MHC-I antigen presentation and MHC-I surface localization oscillated in a circadian manner, which was lost upon deletion of Dec2 in vitro. We conclude that Dec2 promotes primary PDAC progression and likely metastatic dormancy through immune evasion.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":"1208-1222.e9"},"PeriodicalIF":9.2,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13519945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147765530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0