Developmental cell最新文献

A cholesterol-dependent switch controls organ-specific metastasis in pancreatic cancer 一种胆固醇依赖性开关控制胰腺癌的器官特异性转移

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-04 DOI: 10.1016/j.devcel.2025.07.001

Julie Haenlin, Almut Schulze

引用次数: 0

PRC2-mediated repression of PIF4 targets beyond the on/off switch of gene expression prc2介导的PIF4抑制目标超出了基因表达的开/关开关

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-04 DOI: 10.1016/j.devcel.2025.07.005

Zhe Wu

引用次数: 0

Stress delays the bloom: ROS sustains condensates to protect the tomato meristem 胁迫延迟开花：活性氧维持凝结物以保护番茄分生组织

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-04 DOI: 10.1016/j.devcel.2025.06.021

Ashwani Kumar Verma, Panagiotis Nikolaou Moschou

引用次数: 0

Cradles of cellular architecture: ER nests as hubs for organelle birth 细胞结构的摇篮：ER巢作为细胞器诞生的枢纽

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-04 DOI: 10.1016/j.devcel.2025.07.007

Dibyayan Maity, Amit S. Joshi

引用次数: 0

Sympathetic innervation maintains the murine quiescent skeletal muscle stem cell pool via perivascular-derived Angpt1 交感神经支配通过血管周围源性Angpt1维持小鼠静止骨骼肌干细胞池

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-08-01 DOI: 10.1016/j.devcel.2025.07.006

Alessio Rotini, Juliette Berthier, Ester Martínez-Sarrà, Gwladys Berge, Teoman Ozturk, Zeynab Koumaiha, Nathalie Didier, Sara Salucci, Olivier Stettler, Marianne Gervais, Romain K. Gherardi, Peggy Lafuste, Frédéric Relaix

{"title":"Sympathetic innervation maintains the murine quiescent skeletal muscle stem cell pool via perivascular-derived Angpt1","authors":"Alessio Rotini, Juliette Berthier, Ester Martínez-Sarrà, Gwladys Berge, Teoman Ozturk, Zeynab Koumaiha, Nathalie Didier, Sara Salucci, Olivier Stettler, Marianne Gervais, Romain K. Gherardi, Peggy Lafuste, Frédéric Relaix","doi":"10.1016/j.devcel.2025.07.006","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.07.006","url":null,"abstract":"Muscle stem cells rely on their niche for maintenance, yet how β-adrenergic innervation regulates these cells remains elusive. Here, we show that sympathetic fibers in skeletal muscle innervate the vascular stem cell niche, specifically targeting β-adrenergic receptors on perivascular cells. We observe that sympathetic denervation leads to vascular remodeling and, concomitantly, reduces the muscle stem cell pool, resulting in tissue repair defects. Mechanistically, we demonstrate that sympathetic denervation reduces perivascular-derived angiopoietin-1, a crucial factor in maintaining the quiescent state of post-natal muscle stem cells. Using pharmacologic and genetic tools, we identify that sympathetic signaling drives angiopoietin-1 production from murine perivascular cells through the stimulation of their β-adrenergic receptors, thereby preserving the quiescent stem cell pool. Collectively, our data identify the molecular and cellular axis coupling skeletal muscle tissue homeostasis and regeneration to sympathetic innervation and β-adrenergic signaling, which are thus key signaling pathways that contribute to satellite cell quiescence.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"723 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-responsive N4-acetylcytidine mRNA modification controls thermosensory flowering in Arabidopsis 温度响应性n4 -乙酰胞苷mRNA修饰控制拟南芥的热感开花

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-07-30 DOI: 10.1016/j.devcel.2025.07.003

Xiaowei Wu, Songyao Zhang, Tingting Su, Yu Zhang, Chui Eng Wong, Lisha Shen, Hao Yu

{"title":"Temperature-responsive N4-acetylcytidine mRNA modification controls thermosensory flowering in Arabidopsis","authors":"Xiaowei Wu, Songyao Zhang, Tingting Su, Yu Zhang, Chui Eng Wong, Lisha Shen, Hao Yu","doi":"10.1016/j.devcel.2025.07.003","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.07.003","url":null,"abstract":"N4-acetylcytidine (ac4C) represents the only acetylation modification on eukaryotic mRNAs. Although ac4C mRNA modification has been recently identified in plants, its biological function in plant response to environmental signals and related underlying mechanisms remain elusive. Here, we show that two ac4C writers, N-acetyltransferase 10A (NAT10A) and NAT10B, determine thermosensory flowering in Arabidopsis by mediating ac4C deposition on the transcripts of FLOWERING LOCUS M (FLM) and their temperature-dependent alternative splicing. NAT10A and NAT10B are rapidly induced by low ambient temperature, resulting in a transcriptome-wide increase in ac4C modification on target transcripts, including FLM. Consequently, elevated ac4C on nascent FLM transcripts impedes the binding of SPLICING FACTOR 1 to FLM to favor the production of the splice variants FLM-δ versus FLM-β, thereby preventing extremely late flowering at low ambient temperature. These findings demonstrate that ac4C RNA modification constitutes an integral part of the flowering regulatory network that effectively responds to changing temperatures.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"139 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptomics identifies chondrocyte differentiation dynamics in vivo and in vitro 单细胞转录组学在体内和体外鉴定软骨细胞分化动力学

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-07-24 DOI: 10.1016/j.devcel.2025.06.031

John E.G. Lawrence, Steven Woods, Kenny Roberts, Dinithi Sumanaweera, Petra Balogh, Tong Li, Alexander V. Predeus, Peng He, Krzysztof Polanski, Elena Prigmore, Elizabeth Tuck, Lira Mamanova, Di Zhou, Simone Webb, Laura Jardine, Xiaoling He, Roger A. Barker, Muzlifah Haniffa, Adrienne M. Flanagan, Matthew J. Young, Sarah A. Teichmann

{"title":"Single-cell transcriptomics identifies chondrocyte differentiation dynamics in vivo and in vitro","authors":"John E.G. Lawrence, Steven Woods, Kenny Roberts, Dinithi Sumanaweera, Petra Balogh, Tong Li, Alexander V. Predeus, Peng He, Krzysztof Polanski, Elena Prigmore, Elizabeth Tuck, Lira Mamanova, Di Zhou, Simone Webb, Laura Jardine, Xiaoling He, Roger A. Barker, Muzlifah Haniffa, Adrienne M. Flanagan, Matthew J. Young, Sarah A. Teichmann","doi":"10.1016/j.devcel.2025.06.031","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.06.031","url":null,"abstract":"Developing in vitro chondrocytes that replicate in vivo development would benefit musculoskeletal disease modeling and regenerative medicine. Although current methodologies have made progress, challenges such as off-target differentiation can result in heterogeneous cell states. Furthermore, the lack of comparison with human embryonic tissue precludes detailed evaluation of in vitro cells. Here, we perform single-cell RNA sequencing (scRNA-seq) of embryonic long bones and combine this with public data to form an atlas of endochondral ossification. We use this to evaluate published in vitro chondrogenesis protocols that use human cell lines, finding variability in cells produced by each. We apply single-nuclear RNA sequencing (snRNA-seq) to our human embryonic stem cell chondrogenesis protocol and perform trajectory alignment with in vivo data to shed light on off-target differentiation in vitro. Using this information, we inhibit FOXO1, a transcription factor active in embryonic osteoblasts and in vitro cells, to increase chondrocyte transcripts in vitro. This work offers a framework for improving in vitro chondrogenesis using developmental data.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"292 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments 尺蠖的迁移有助于变形虫细胞适应高粘附环境

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-07-24 DOI: 10.1016/j.devcel.2025.06.035

Jinqiang Yu, Wenbo Zhou, Dongju Wang, Xiaoting Chao, Taoran Wang, Yuheng Liu, Haozhi Qu, Bingyu Liu, Dong Li, Wei Wang, Hongyu Wang, Huaqing Cai, Li Yu

{"title":"Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments","authors":"Jinqiang Yu, Wenbo Zhou, Dongju Wang, Xiaoting Chao, Taoran Wang, Yuheng Liu, Haozhi Qu, Bingyu Liu, Dong Li, Wei Wang, Hongyu Wang, Huaqing Cai, Li Yu","doi":"10.1016/j.devcel.2025.06.035","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.06.035","url":null,"abstract":"Cells dynamically adapt their migration modes to environmental conditions, but their response to sticky surfaces, where they risk becoming immobilized, remains unclear. In our study, we discovered that strong adhesion prompts substantial changes in Dictyostelium discoideum, leading to “inchworm migration,” a novel subtype of amoeboid migration. This adaptation involves minimal contact between the cell and the surface, with the cell body standing upright and twisting, followed by rapid reattachment for directed movement. Concurrently, the cells shed migrasomes loaded with negatively charged molecules and adhesion receptors, controlling their adhesion traits to resume pseudopod migration. We identify the repurposing of cytokinesis machinery for migration mode shifting and selective membrane shedding as a crucial mechanism. Neutrophils also exhibit inchworm migration under strong adhesion, suggesting its broader application among amoeboid cells in adapting to high-adhesion environments. Our findings illuminate a programmed, adaptive response in amoeboid cells to navigate effectively through strongly adhesive terrains.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"47 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marsupial single-cell transcriptomics identifies temporal diversity in mammalian developmental programs 有袋动物单细胞转录组学鉴定哺乳动物发育程序的时间多样性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-07-24 DOI: 10.1016/j.devcel.2025.06.037

Sergio Menchero, Christopher Barrington, Clare Critcher, Wazeer Varsally, Gregorio Alanis-Lobato, Kathy K. Niakan, James M.A. Turner

{"title":"Marsupial single-cell transcriptomics identifies temporal diversity in mammalian developmental programs","authors":"Sergio Menchero, Christopher Barrington, Clare Critcher, Wazeer Varsally, Gregorio Alanis-Lobato, Kathy K. Niakan, James M.A. Turner","doi":"10.1016/j.devcel.2025.06.037","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.06.037","url":null,"abstract":"Single-cell transcriptomics has demonstrated conserved and divergent programs of organogenesis in mammals, but existing studies have focused on eutherians. Marsupials exhibit short gestation and complete development externally, necessitating accelerated differentiation of anterior features required for locomotion and feeding. Hence, they represent a unique outgroup for studying temporal shifts in development, known as heterochrony. Here, we generate a single-cell transcriptomic atlas of gastrulation and early organogenesis in a marsupial, the opossum Monodelphis domestica. We identify previously undocumented tissues undergoing heterochrony and find that transcriptional programs that form anterior structures initiate earlier and progress faster relative to eutherians. The result is uncoupling of transcriptional and morphological timelines, revealing unforeseen diversity in mammalian developmental sequences. Using our transcriptomic dataset, we identified translation as a candidate control mechanism by which anterior prioritization is achieved. Our findings provide insight into the asynchronous progression of developmental programs in marsupials.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"61 6 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Telocytes deliver essential Wnts directly to murine intestinal stem cells via synapse-like contacts 远端细胞通过突触样接触将必需的wnt直接传递给小鼠肠干细胞

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-07-23 DOI: 10.1016/j.devcel.2025.06.040

Gediminas Greicius, Lorenz Mittermeier, Ruanyi Liang, Kristmundur Sigmundsson, Yarn Kit Chan, Pei-Ju Liao, Alexander Ludwig, David M. Virshup

{"title":"Telocytes deliver essential Wnts directly to murine intestinal stem cells via synapse-like contacts","authors":"Gediminas Greicius, Lorenz Mittermeier, Ruanyi Liang, Kristmundur Sigmundsson, Yarn Kit Chan, Pei-Ju Liao, Alexander Ludwig, David M. Virshup","doi":"10.1016/j.devcel.2025.06.040","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.06.040","url":null,"abstract":"Spatial and temporal control of Wnt delivery to the intestinal stem cell niche regulates intestinal homeostasis. Telocytes, specialized stromal cells with characteristic long, thin cytoplasmic protrusions, produce essential Wnts for the development and maintenance of this niche. However, how Wnts travel from telocytes to stem cells in the gut remains unclear. Fluorescence and electron microscopy of murine telocytes co-cultured with intestinal organoids identified specialized telocyte extensions that transport and locally secrete Wnts on microvesicles and make intimate contacts with epithelial cells, reminiscent of neuronal contact-based signaling. Investigating the potential role of synapse-forming and plasma membrane-associated platform proteins, we found that depletion of either KANK1 or Liprins from telocytes markedly reduced their filopodia, compromised WNT2 presentation to epithelial cells, and impaired telocyte-dependent organoid growth. Characteristic telocyte structures facilitate Wnt delivery to the intestinal stem cell niche via synapse-like contacts.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"46 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0