Developmental cell最新文献

Transcriptional conservation and evolutionary divergence of cell types across mammalian hypothalamus development

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-08 DOI: 10.1016/j.devcel.2025.03.009

Zhen-Hua Chen, Taotao Bruce Pan, Yu-Hong Zhang, Ben Wang, Xue-Lian Sun, Meixi Gao, Yang Sun, Mingrui Xu, Shuhui Han, Xiang Shi, Felipe Correa-da-Silva, Chenlu Yang, Junfu Guo, Haoda Wu, Yu Zheng Li, Xiu-Qin Liu, Fei Gao, Zhiheng Xu, Shengjin Xu, Xin Liu, Qing-Feng Wu

{"title":"Transcriptional conservation and evolutionary divergence of cell types across mammalian hypothalamus development","authors":"Zhen-Hua Chen, Taotao Bruce Pan, Yu-Hong Zhang, Ben Wang, Xue-Lian Sun, Meixi Gao, Yang Sun, Mingrui Xu, Shuhui Han, Xiang Shi, Felipe Correa-da-Silva, Chenlu Yang, Junfu Guo, Haoda Wu, Yu Zheng Li, Xiu-Qin Liu, Fei Gao, Zhiheng Xu, Shengjin Xu, Xin Liu, Qing-Feng Wu","doi":"10.1016/j.devcel.2025.03.009","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.009","url":null,"abstract":"The hypothalamus, an “ancient” subcortical brain structure, maintains physiological homeostasis and controls native behaviors. The evolution of homeostatic regulation and behavioral control in mammals may rely on adaptable neuronal identity establishment but conserved neural patterning mechanisms during neurodevelopment. Here, we combined single-cell, single-nucleus, and spatial transcriptomic datasets to map the spatial patterning of diverse progenitor domains and reconstruct their neurogenic lineages in the developing human and mouse hypothalamus. While the regional organizers orchestrating neural patterning are conserved between primates and rodents, we identified a human-enriched neuronal subtype and found a substantial increase in neuromodulatory gene expression among human neurons. Furthermore, cross-species comparison demonstrated a potential redistribution of two neuroendocrine neuronal subtypes and a shift in inter-transmitter and transmitter-peptide coupling within hypothalamic dopamine neurons. Together, our study lays a critical foundation for understanding cellular development and evolution of the mammalian hypothalamus.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"92 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143798387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neighbor cells restrain furrowing during Xenopus epithelial cytokinesis

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-08 DOI: 10.1016/j.devcel.2025.03.010

Jennifer Landino, Eileen Misterovich, Lotte van den Goor, Babli Adhikary, Shahana Chumki, Lance A. Davidson, Ann L. Miller

{"title":"Neighbor cells restrain furrowing during Xenopus epithelial cytokinesis","authors":"Jennifer Landino, Eileen Misterovich, Lotte van den Goor, Babli Adhikary, Shahana Chumki, Lance A. Davidson, Ann L. Miller","doi":"10.1016/j.devcel.2025.03.010","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.010","url":null,"abstract":"Cytokinesis challenges epithelial tissue homeostasis by generating forces that pull on neighboring cells. Junction reinforcement at the furrow in Xenopus epithelia regulates the speed of furrowing, suggesting that cytokinesis is subject to resistive forces from epithelial neighbors. We show that contractility factors accumulate near the furrow in neighboring cells, and increasing neighbor cell stiffness slows furrowing. Optogenetically increasing contractility in one or both neighbor cells slows furrowing or induces cytokinetic failure. Uncoupling mechanotransduction between dividing cells and their neighbors increases the furrow ingression rate, alters topological cell packing following cytokinesis, and impairs barrier function at the furrow. Computational modeling validates our findings and provides additional insights about epithelial mechanics during cytokinesis. We conclude that forces from the cytokinetic array must be carefully balanced with restraining forces generated by neighbor cells to regulate the speed and success of cytokinesis and maintain epithelial homeostasis.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"38 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143798389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

mTORC1 shutdown unleashes TFEB to drive triple-negative breast cancer invasion

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-07 DOI: 10.1016/j.devcel.2025.03.006

Michalis Gounis, Hellyeh Hamidi, Johanna Ivaska

引用次数: 0

Controlling pyroptosis through post-translational modifications of gasdermin D

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-07 DOI: 10.1016/j.devcel.2025.02.005

Na Zhang, Daichao Xu

引用次数: 0

Mechanisms of ferroptosis sensitization and resistance

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-07 DOI: 10.1016/j.devcel.2025.02.004

Weaverly Colleen Lee, Scott J. Dixon

引用次数: 0

Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-03 DOI: 10.1016/j.devcel.2025.03.008

Nai Yang Yeat, Li-Heng Liu, Yu-Hsuan Chang, Charles Pin-Kuang Lai, Ruey-Hwa Chen

{"title":"Bro1 proteins determine tumor immune evasion and metastasis by controlling secretion or degradation of multivesicular bodies","authors":"Nai Yang Yeat, Li-Heng Liu, Yu-Hsuan Chang, Charles Pin-Kuang Lai, Ruey-Hwa Chen","doi":"10.1016/j.devcel.2025.03.008","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.008","url":null,"abstract":"Exosomes play pleiotropic tumor-promoting functions and are secreted by fusion of multivesicular bodies (MVBs) with the plasma membrane. However, MVBs are also directed to lysosomes for degradation, and the mechanism controlling different fates of MVBs remains elusive. Here, we show that the pro-tumor protein WDR4 enhances exosome secretion from mouse and human cancer cells through degrading the endosomal sorting complex required for transport (ESCRT)-associated Bro1-family protein PTPN23. Mechanistically, PTPN23 and ALIX compete for binding to syntenin, thereby directing MVBs toward degradation and secretion, respectively. ALIX, but not PTPN23, recruits actin-capping proteins CAPZA1/CAPZB to prevent branched filamentous actin (F-actin) accumulation around MVBs, thus enabling MVBs trafficking to the cell periphery for secretion. Functionally, WDR4/ALIX-dependent exosomes load a set of pro-tumor proteins through LAMP2A, thereby potentiating metastasis and immune evasion in mice. Our study highlights a previously unappreciated coupling between the biogenesis mechanism and the fate decision of MVBs and its importance in determining exosomal cargos, which have a profound impact on tumor progression.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"224 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS burst prolongs transcriptional condensation to slow shoot apical meristem maturation and achieve heat-stress resilience in tomato

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-02 DOI: 10.1016/j.devcel.2025.03.007

Xiaozhen Huang, Nan Xiao, Yue Xie, Cao Xu

{"title":"ROS burst prolongs transcriptional condensation to slow shoot apical meristem maturation and achieve heat-stress resilience in tomato","authors":"Xiaozhen Huang, Nan Xiao, Yue Xie, Cao Xu","doi":"10.1016/j.devcel.2025.03.007","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.007","url":null,"abstract":"The transition of the shoot apical meristem (SAM) from vegetative growth to flowering, a key step of angiosperm reproductive success, is highly vulnerable to heat stress. Overproduction of reactive oxygen species (ROS) is a hallmark of such environmental stresses, but how SAM exploits the extra ROS to achieve heat-stress resilience is largely unknown. Here, we report that tomato plants respond to heat-induced ROS burst by slowing down SAM maturation and lengthening the vegetative state to achieve heat resilience. Heat-induced extra ROS prolonged the transcriptional condensation status of TERMINATING FLOWER (TMF), a prion-like transcription repressor that undergoes phase separation by sensing hydrogen peroxide (H2O2), therefore temporarily delaying activation of flowering transition and extending vegetative growth. Loss-of-function of TMF, or base editing of a single cysteine residue that senses H2O2, abolishes heat resilience. Our findings demonstrate that transcriptional reprogramming triggered by ROS might be a molecular basis of plant developmental plasticity underlying heat-stress resilience.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"104 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIF2C promotes paclitaxel resistance by depolymerizing polyglutamylated microtubules

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-03-28 DOI: 10.1016/j.devcel.2025.03.004

Yuan-Shao Pao, Kuan-Ju Liao, Ya-Chia Shiau, Ming-Hong Chao, Mu-Chun Li, Li-Mei Lin, Hsin-Huei Chang, Hung-Wei Yeh, Yi-Ju Chen, Yu-Ting Chiu, Max Yu-Chen Pan, Yu-Hsuan Chang, Shih-Yu Shen, Shu-Yu Lin, Hui-Chun Cheng, Yu-Chun Lin, Yuh-Ju Sun, Ching-Chuan Kuo, Hsing-Pang Hsieh, Lily Hui-Ching Wang

{"title":"KIF2C promotes paclitaxel resistance by depolymerizing polyglutamylated microtubules","authors":"Yuan-Shao Pao, Kuan-Ju Liao, Ya-Chia Shiau, Ming-Hong Chao, Mu-Chun Li, Li-Mei Lin, Hsin-Huei Chang, Hung-Wei Yeh, Yi-Ju Chen, Yu-Ting Chiu, Max Yu-Chen Pan, Yu-Hsuan Chang, Shih-Yu Shen, Shu-Yu Lin, Hui-Chun Cheng, Yu-Chun Lin, Yuh-Ju Sun, Ching-Chuan Kuo, Hsing-Pang Hsieh, Lily Hui-Ching Wang","doi":"10.1016/j.devcel.2025.03.004","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.004","url":null,"abstract":"The long-term effectiveness of paclitaxel is limited by chemoresistance. In this study, we elucidate the molecular mechanism by which kinesin family member 2C (KIF2C), a well-known microtubule depolymerase, contributes to the development of chemoresistance in triple-negative breast cancer (TNBC). We observed elevated levels of KIF2C, tubulin tyrosination, and polyglutamylation in human and mouse breast cancer cells resistant to paclitaxel. Additionally, these chemoresistant cells possessed cross-resistance to diverse microtubule-targeting agents (MTAs). We demonstrated that KIF2C preferentially depolymerizes polyglutamylated tubulin, even in the presence of paclitaxel. To counter this, we developed 7S9, a chemical inhibitor of KIF2C, that prohibits the dissociation of KIF2C from microtubules. The combination of 7S9 and paclitaxel significantly reduced tumorigenesis in chemoresistant TNBC model in mice. Moreover, 7S9 diminished cancer cell chemoresistance to several clinically available MTAs. Our findings elucidate the molecular mechanism of KIF2C-mediated chemoresistance and highlight KIF2C as a promising target for combating cross-resistance in TNBC.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"7 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ER nests are specialized ER subdomains in Arabidopsis where peroxisomes and lipid droplets form

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-03-28 DOI: 10.1016/j.devcel.2025.03.005

Zachary J. Wright, Nathan E. Tharp, Bonnie Bartel

引用次数: 0

VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-03-26 DOI: 10.1016/j.devcel.2025.03.001

Rory Osborne, Anne-Marie Labandera, Alex J. Ryder, Anastasia Kanali, Tianyuan Xu, Oluwatunmise Akintewe, Maximillian A. Schwarze, Christian D. Morgan, Sjon Hartman, Eirini Kaiserli, Daniel J. Gibbs

{"title":"VRN2-PRC2 facilitates light-triggered repression of PIF signaling to coordinate growth in Arabidopsis","authors":"Rory Osborne, Anne-Marie Labandera, Alex J. Ryder, Anastasia Kanali, Tianyuan Xu, Oluwatunmise Akintewe, Maximillian A. Schwarze, Christian D. Morgan, Sjon Hartman, Eirini Kaiserli, Daniel J. Gibbs","doi":"10.1016/j.devcel.2025.03.001","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.001","url":null,"abstract":"VERNALIZATION2 (VRN2) is a flowering plant-specific subunit of the polycomb-repressive complex 2 (PRC2), a conserved eukaryotic holoenzyme that represses gene expression by depositing the histone H3 lysine 27 trimethylation (H3K27me3) mark in chromatin. Previous work established VRN2 as an oxygen-regulated target of the N-degron pathway that may function as a sensor subunit connecting PRC2 activity to the perception of endogenous and environmental cues. Here, we show that VRN2 is enriched in the hypoxic shoot apex and emerging leaves of Arabidopsis, where it negatively regulates growth by establishing a stable and conditionally repressed chromatin state in key PHYTOCHROME INTERACTING FACTOR (PIF)-regulated genes that promote cell expansion. This function is required to keep these genes poised for repression via a light-responsive signaling cascade later in leaf development. Thus, we identify VRN2-PRC2 as a core component of a developmentally and spatially encoded epigenetic mechanism that coordinates plant growth through facilitating the signal-dependent suppression of PIF signaling.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"27 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0