Developmental cell最新文献

Calcium-responsive phosphorylation of SlLHP1b epigenetically suppresses auxin synthesis to control drought-induced flower drop in tomato SlLHP1b的钙响应磷酸化表观遗传抑制生长素合成以控制干旱诱导的番茄落花

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-02 DOI: 10.1016/j.devcel.2025.05.006

Siqi Ge, Sai Wang, Xianfeng Liu, Lina Cheng, Ruizhen Li, Yang Liu, Yue Cai, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Mingfang Qi, Tianlai Li, Tao Xu

{"title":"Calcium-responsive phosphorylation of SlLHP1b epigenetically suppresses auxin synthesis to control drought-induced flower drop in tomato","authors":"Siqi Ge, Sai Wang, Xianfeng Liu, Lina Cheng, Ruizhen Li, Yang Liu, Yue Cai, Sida Meng, Changhua Tan, Cai-Zhong Jiang, Mingfang Qi, Tianlai Li, Tao Xu","doi":"10.1016/j.devcel.2025.05.006","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.006","url":null,"abstract":"Drought causes the abscission of flowers and fruits, thereby reducing yields. Although trimethylation of histone H3 in the lysine 27 (H3K27me3)-dependent pathway has been shown to control drought responses, the mechanisms underlying drought-induced abscission remain unknown. In this study, we showed that drought-induced calcineurin B-like11 (SlCBL11) upregulation activated the CBL-interacting protein kinase10 (SlCIPK10) to phosphorylate like heterochromatin protein 1b (SlLHP1b) at Thr387 and Thr389, respectively, in stamens of tomato (Solanum lycopersicum). Phosphorylation at Thr389 improved SlLHP1b stability, and phosphorylation at Thr387/Thr389 enhanced SlLHP1b binding ability to H3K27me3. Hence, SlLHP1b-dependent epigenetic silencing of the expression of SlYUC in stamens was promoted, which decreased the auxin content in stamens, causing the disruption of the auxin gradient in the abscission zone, and eventually flower drop in tomatoes. Overall, we showed a mechanism by which Ca2+ signaling controls H3K27me3 through the SlCBL11-SlCIPK10-SlLHP1b module to inhibit auxin synthesis during drought-induced flower drop.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"14 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair ATG9A和ARFIP2共同控制PI4P水平，参与溶酶体修复

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-02 DOI: 10.1016/j.devcel.2025.05.007

Stefano De Tito, Eugenia Almacellas, Daniel Dai Yu, Emily Millard, Wenxin Zhang, Cecilia de Heus, Christophe Queval, Javier H. Hervás, Enrica Pellegrino, Ioanna Panagi, Ditte Fogde, Teresa L.M. Thurston, Judith Klumperman, Maximiliano Gutierrez, Sharon A. Tooze

{"title":"ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair","authors":"Stefano De Tito, Eugenia Almacellas, Daniel Dai Yu, Emily Millard, Wenxin Zhang, Cecilia de Heus, Christophe Queval, Javier H. Hervás, Enrica Pellegrino, Ioanna Panagi, Ditte Fogde, Teresa L.M. Thurston, Judith Klumperman, Maximiliano Gutierrez, Sharon A. Tooze","doi":"10.1016/j.devcel.2025.05.007","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.007","url":null,"abstract":"Lysosome damage activates multiple pathways to prevent lysosome-dependent cell death, including a repair mechanism involving endoplasmic reticulum (ER)-lysosome membrane contact sites, phosphatidylinositol 4-kinase-2a (PI4K2A), phosphatidylinositol-4 phosphate (PI4P), and oxysterol-binding protein-like proteins (OSBPLs) lipid transfer proteins. PI4K2A localizes to the trans-Golgi network and endosomes, yet how it is delivered to damaged lysosomes remains unknown. During acute sterile damage and damage caused by intracellular bacteria, we show that ATG9A-containing vesicles perform a critical role in delivering PI4K2A to damaged lysosomes. ADP ribosylation factor interacting protein 2 (ARFIP2), a component of ATG9A vesicles, binds and sequesters PI4P on lysosomes, balancing OSBPL-dependent lipid transfer and promoting the retrieval of ATG9A vesicles through the recruitment of the adaptor protein complex-3 (AP-3). Our results identify a role for mobilized ATG9A vesicles and ARFIP2 in lysosome homeostasis after damage and bacterial infection.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"81 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rice stripe virus NS3 uses the host signaling pathways to control pathogenicity 水稻条纹病毒NS3利用寄主信号通路控制致病性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-06-02 DOI: 10.1016/j.devcel.2025.04.023

Xinjian Zhuang, Chenwei Feng, Yanhong Hua, Tianxiao Gu, Xiao Guo, Zhen He, Kai Xu, Fang Liu, Peter Moffett, Kun Zhang

{"title":"Rice stripe virus NS3 uses the host signaling pathways to control pathogenicity","authors":"Xinjian Zhuang, Chenwei Feng, Yanhong Hua, Tianxiao Gu, Xiao Guo, Zhen He, Kai Xu, Fang Liu, Peter Moffett, Kun Zhang","doi":"10.1016/j.devcel.2025.04.023","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.023","url":null,"abstract":"Viruses pose a significant threat to animal and plant health worldwide. How viruses adapt to vectors and hosts more sustainably remains unclear. Rice stripe virus (RSV) is a devastating rice-infecting RNA virus and is exclusively transmitted by Laodelphax striatellus (Fallén). During the early stages of viral infection, limited NS3 protein undergoes self-interaction, thereby suppressing the host’s antiviral RNA interference (RNAi) pathway. Meanwhile, RSV-induced Ca2+ signals activate the OsSnRK3.25-OsCBL1/3-OsRBOHs-mediated reactive oxygen species (ROS) burst and programmed cell death (PCD). RSV exhibits strong pathogenicity and transmissibility. In later stages, an abundance of NS3 interacts with OsSnRK3.25 and undergoes phosphorylation, which enhances the host antiviral RNAi pathway while concurrently disrupting the endogenous OsSnRK3.25-OsCBL1/3-OsRBOHs signaling. Here, RSV demonstrates reduced pathogenicity and transmissibility. Thus, the virus fine-tunes its pathogenicity and transmissibility by NS3 phosphorylation and hijacking OsSnRK3.25, sustaining a delicate balance between virus-host-vector interactions. This study identifies a co-survival strategy within virus-vector-host triple interactions.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"13 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional transcription factor GhMYB4 orchestrates transition from elongation to secondary cell wall synthesis trade-off in cotton fiber 双功能转录因子GhMYB4协调棉纤维从伸长到次级细胞壁合成的过渡

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-30 DOI: 10.1016/j.devcel.2025.05.004

Lu Qiao, Haozhe Tan, Mengling Sun, Xiao Zhang, Shenghua Xiao, Zhengxiu Ye, Zhiwei Chen, Xianlong Zhang, Lili Tu

{"title":"Bifunctional transcription factor GhMYB4 orchestrates transition from elongation to secondary cell wall synthesis trade-off in cotton fiber","authors":"Lu Qiao, Haozhe Tan, Mengling Sun, Xiao Zhang, Shenghua Xiao, Zhengxiu Ye, Zhiwei Chen, Xianlong Zhang, Lili Tu","doi":"10.1016/j.devcel.2025.05.004","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.05.004","url":null,"abstract":"The integration of cell wall expansion and reinforcement is vital for plant cell wall development. Cotton fiber is characterized by the synchronized development of fiber growth and cell wall formation, providing an excellent system for investigating plant cell walls. Here, we found that the often-overlooked transition stage coordinates fiber elongation and secondary cell wall (SCW) synthesis through antagonistic effects of the transcription factor GhMYB4. Knockout of GhMYB4 produced longer and finer fibers, contrasting with overexpression of GhMYB4. We show that GhMYB4 represses fiber elongation through fatty acid and brassinosteroid pathways involving a negative feedback loop with GhRAP2 and GhTCP15, while activating SCW synthesis by inducing cellulose biosynthesis in succession with the GhMYB52-GhFSN1-GhILR3 module. We identify that the bifunctionality of GhMYB4 depends on its interaction with different cis-elements and is executed through distinct transcriptional regulation motifs. Our findings propose a strategy to improve fiber quality by orchestrating wall expansion and stiffness.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"37 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144176811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An antisense RNA forms R-loop to facilitate the transcription of CBF genes and plant cold acclimation 反义RNA形成r环，促进CBF基因转录和植物冷驯化

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-28 DOI: 10.1016/j.devcel.2025.04.028

Jianhang Sun, Xinyue Zhao, Diyi Fu, Yiting Shi, Shuhua Yang, Yijun Qi

{"title":"An antisense RNA forms R-loop to facilitate the transcription of CBF genes and plant cold acclimation","authors":"Jianhang Sun, Xinyue Zhao, Diyi Fu, Yiting Shi, Shuhua Yang, Yijun Qi","doi":"10.1016/j.devcel.2025.04.028","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.028","url":null,"abstract":"Cold acclimation is an adaption used by many plants to survive freezing temperatures and involves rapid induction of the expression of three C-REPEAT-BINDING FACTOR (CBF) genes. We have previously annotated long non-coding RNAs (lncRNAs) including natural antisense transcripts (NAT-lncRNAs) on a genome-wide scale in Arabidopsis and identified a NAT-lncRNA transcribed from the antisense strand between CBF1 and CBF3. We named it CAS for CBF antisense transcript. CAS corresponds to two of the three β isoforms of the lncRNA SVALKA (SVK) previously reported. We show that CAS/SVK is induced to a high level shortly after cold exposure and positively controls CBF1 and CBF3 expression during acclimation, which in turn confers acquired freezing tolerance of plants. CAS/SVK organizes an R-loop to reduce nucleosome occupancy, thus facilitating CBF1 and CBF3 transcription. Our study demonstrates a distinct role of CAS/SVK in facilitating neighboring gene transcription and underscores the importance of lncRNA-mediated control in cold acclimation.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"50 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myocardium and endocardium of the early mammalian heart tube arise from independent multipotent lineages specified at the primitive streak 早期哺乳动物心管的心肌和心内膜起源于独立的多能系，指定为原始条纹

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-27 DOI: 10.1016/j.devcel.2025.05.002

引用次数: 0

Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis 在爪蟾气管-食管形态发生的先天性异常中，Vangl-Celsr极性复合物的内体运输被破坏

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-23 DOI: 10.1016/j.devcel.2025.04.026

Nicole A. Edwards, Scott A. Rankin, Adhish Kashyap, Alissa Warren, Zachary N. Agricola, Alan P. Kenny, Matthew Kofron, Yufeng Shen, Wendy K. Chung, Aaron M. Zorn

{"title":"Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis","authors":"Nicole A. Edwards, Scott A. Rankin, Adhish Kashyap, Alissa Warren, Zachary N. Agricola, Alan P. Kenny, Matthew Kofron, Yufeng Shen, Wendy K. Chung, Aaron M. Zorn","doi":"10.1016/j.devcel.2025.04.026","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.026","url":null,"abstract":"Disruptions in foregut morphogenesis can result in life-threatening conditions where the trachea and esophagus fail to separate, such as esophageal atresia (EA) and tracheoesophageal fistulas (TEFs). The developmental basis of these congenital anomalies is poorly understood, but recent genome sequencing reveals that de novo variants in intracellular trafficking genes are enriched in EA/TEF patients. Here, we confirm that mutation of orthologous genes in Xenopus disrupts trachea-esophageal separation similar to EA/TEF patients. The Rab11a recycling endosome pathway is required to localize Vangl-Celsr polarity complexes at the luminal cell surface where opposite sides of the foregut tube fuse. Partial loss of endosomal trafficking or Vangl-Celsr complexes disrupts epithelial polarity and cell division orientation. Mutant cells accumulate at the fusion point, fail to relocalize cadherin, and do not separate into distinct trachea and esophagus. These data provide insights into the mechanisms of congenital anomalies and general paradigms of tissue fusion during organogenesis.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"33 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoids 工程血管诱导多能干细胞衍生的胰岛类器官功能成熟

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-23 DOI: 10.1016/j.devcel.2025.04.024

Yesl Jun, Kim-Vy Nguyen-Ngoc, Somesh Sai, R. Hugh F. Bender, Winnie Gong, Vira Kravets, Han Zhu, Christopher J. Hatch, Michael Schlichting, Roberto Gaetani, Medhavi Mallick, Stephanie J. Hachey, Karen L. Christman, Steven C. George, Christopher C.W. Hughes, Maike Sander

{"title":"Engineered vasculature induces functional maturation of pluripotent stem cell-derived islet organoids","authors":"Yesl Jun, Kim-Vy Nguyen-Ngoc, Somesh Sai, R. Hugh F. Bender, Winnie Gong, Vira Kravets, Han Zhu, Christopher J. Hatch, Michael Schlichting, Roberto Gaetani, Medhavi Mallick, Stephanie J. Hachey, Karen L. Christman, Steven C. George, Christopher C.W. Hughes, Maike Sander","doi":"10.1016/j.devcel.2025.04.024","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.024","url":null,"abstract":"Blood vessels play a critical role in pancreatic islet function, yet current methods for deriving islet organoids from human pluripotent stem cells (SC-islets) lack vasculature. We engineered three-dimensional (3D) vascularized SC-islet organoids by assembling SC-islet cells, human primary endothelial cells (ECs), and fibroblasts in a non-perfused model and a microfluidic device with perfused vessels. Vasculature improved stimulus-dependent Ca2+ influx into SC-β cells, a hallmark of β cell function that is blunted in non-vascularized SC-islets. Moreover, vascularization accelerated diabetes reversal post engraftment of a subtherapeutic SC-islet dose into mice. We show that vasculature leads to the formation of an islet-like basement membrane that contributes to the functional improvement of SC-β cells. Furthermore, single-cell RNA sequencing (scRNA-seq) data predicted BMP2/4-BMPR2 signaling from ECs to SC-β cells, and correspondingly, BMP4 enhanced the SC-β cell Ca2+ response and insulin secretion. Vascularized SC-islets will enable further studies of crosstalk between β cells and ECs and will serve as an in vitro platform for disease modeling and therapeutic testing.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"20 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-induced pro-inflammatory glioblastoma stem cells secrete TNFAIP6 to enhance tumor growth and induce suppressive macrophages 应激诱导的促炎性胶质母细胞瘤干细胞分泌TNFAIP6促进肿瘤生长并诱导抑制巨噬细胞

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-21 DOI: 10.1016/j.devcel.2025.04.027

Danling Gu, Lang Hu, Kailin Yang, Wei Yuan, Danyang Shan, Jiancheng Gao, Jiahuang Li, Ryan C. Gimple, Deobrat Dixit, Zhe Zhu, Daqi Li, Qiulian Wu, Zhumei Shi, Yingyi Wang, Ningwei Zhao, Kun Yang, Junfei Shao, Fan Lin, Qianghu Wang, Guangfu Jin, Xiuxing Wang

{"title":"Stress-induced pro-inflammatory glioblastoma stem cells secrete TNFAIP6 to enhance tumor growth and induce suppressive macrophages","authors":"Danling Gu, Lang Hu, Kailin Yang, Wei Yuan, Danyang Shan, Jiancheng Gao, Jiahuang Li, Ryan C. Gimple, Deobrat Dixit, Zhe Zhu, Daqi Li, Qiulian Wu, Zhumei Shi, Yingyi Wang, Ningwei Zhao, Kun Yang, Junfei Shao, Fan Lin, Qianghu Wang, Guangfu Jin, Xiuxing Wang","doi":"10.1016/j.devcel.2025.04.027","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.027","url":null,"abstract":"Glioblastoma (GBM) is the most aggressive primary intracranial tumor, with glioblastoma stem cells (GSCs) enforcing the intratumoral hierarchy. The inflammatory microenvironment influences tumor development at varying stages, while the underlying mechanism of GSCs facing pro-inflammatory stress remains unclear. Here, we show that, in human GBM, pro-inflammatory stress from pro-inflammatory macrophages (pTAMs) maintains GSC proliferation and self-renewal. Tumor necrosis factor alpha-induced protein 6 (TNFAIP6), as a responder in patient-derived GSCs to pro-inflammatory stress tumor necrosis factor alpha (TNF-α) from human pTAMs, promotes tumor growth through binding epidermal growth factor (EGF) and prolonging EGF receptor (EGFR)-phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT) signaling activation. Meanwhile, pro-inflammatory stress-induced patient-derived GSCs secrete TNFAIP6 to transform macrophage phenotype from pTAMs to inflammatory-suppressive macrophages (sTAMs). Collectively, pharmacological or genetic disruption of TNFAIP6 autocrine and paracrine communication between patient-derived GSCs and TAMs inhibited GSC proliferation and self-renewal in vitro and in patient-derived xenograft tumor-bearing mice, suggesting that TNFAIP6 is an effective target for GBM therapy.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"40 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144104096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human yolk sac-derived innate lymphoid-biased multipotent progenitors emerge prior to hematopoietic stem cell formation 人卵黄囊衍生的先天淋巴偏向性多能祖细胞先于造血干细胞形成

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-20 DOI: 10.1016/j.devcel.2025.05.003

Yanli Ni, Guoju You, Yandong Gong, Xiaoyu Su, Yuan Du, Xiaoshuang Wang, Xiaochen Ding, Qingfeng Fu, Man Zhang, Tao Cheng, Yu Lan, Bing Liu, Chen Liu

引用次数: 0