Developmental cell最新文献

Human-specific contributors to cleavage-stage embryonic arrest during maternal to zygotic transition 在母体到合子的转变过程中卵裂期胚胎停止的人类特异性贡献者

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-05 DOI: 10.1016/j.devcel.2025.04.007

Mehmet-Yunus Comar, Bernardo Oldak, Jacob H. Hanna

引用次数: 0

What insights can spatiotemporal esophageal atlases and deep learning bring to engineering the esophageal mucosa? 时空食道地图集和深度学习能为食道黏膜工程带来哪些启示？

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-05 DOI: 10.1016/j.devcel.2025.04.009

Shuyan Wang, Nianping Liu, Kun Qu

引用次数: 0

Stemness in flux: Dissecting intestinal crypt organization with multimodal approaches 流动中的干性：用多模式方法解剖肠隐窝组织

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-05 DOI: 10.1016/j.devcel.2025.04.003

Maria Azkanaz, Dimitrios Laskaris, Jacco van Rheenen

引用次数: 0

Paraoxonase-like APMAP maintains endoplasmic-reticulum-associated lipid and lipoprotein homeostasis 对氧磷酶样APMAP维持内质网相关的脂质和脂蛋白稳态

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-02 DOI: 10.1016/j.devcel.2025.04.008

Blessy Paul, Holly Merta, Rupali Ugrankar-Banerjee, Monica R. Hensley, Son Tran, Goncalo Dias do Vale, Lauren Zacherias, Charles K. Hewett, Jeffrey G. McDonald, Joan Font-Burgada, Thomas P. Mathews, Steven A. Farber, W. Mike Henne

{"title":"Paraoxonase-like APMAP maintains endoplasmic-reticulum-associated lipid and lipoprotein homeostasis","authors":"Blessy Paul, Holly Merta, Rupali Ugrankar-Banerjee, Monica R. Hensley, Son Tran, Goncalo Dias do Vale, Lauren Zacherias, Charles K. Hewett, Jeffrey G. McDonald, Joan Font-Burgada, Thomas P. Mathews, Steven A. Farber, W. Mike Henne","doi":"10.1016/j.devcel.2025.04.008","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.008","url":null,"abstract":"Oxidative stress perturbs lipid homeostasis and contributes to metabolic diseases. Though ignored when compared with mitochondrial oxidation, the endoplasmic reticulum (ER) generates reactive oxygen species requiring antioxidant quality control. Using multi-organismal profiling featuring Drosophila, zebrafish, and mammalian hepatocytes, here we characterize the paraoxonase-like C20orf3/adipocyte plasma-membrane-associated protein (APMAP) as an ER-localized antioxidant that suppresses ER lipid oxidation to safeguard ER function. APMAP-depleted cells exhibit defective ER morphology, ER stress, and lipid peroxidation dependent on ER-oxidoreductase 1α (ERO1A), as well as sensitivity to ferroptosis and defects in ApoB-lipoprotein homeostasis. Similarly, organismal APMAP depletion in Drosophila and zebrafish perturbs ApoB-lipoprotein homeostasis. Strikingly, APMAP loss is rescued with chemical antioxidant N-acetyl-cysteine (NAC). Lipidomics identifies that APMAP loss elevates phospholipid peroxidation and boosts ceramides—signatures of lipid stress. Collectively, we propose that APMAP is an ER-localized antioxidant that promotes lipid and lipoprotein homeostasis in the ER network.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"1 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143897892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sumoylated Etv1 establishes mouse mammary cancer stem cells that support tumorigenesis by non-stem cancer cells Sumoylated Etv1建立小鼠乳腺癌干细胞，支持非干细胞癌细胞的肿瘤发生

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-05-01 DOI: 10.1016/j.devcel.2025.04.005

Zhijie Li, Kelsey E. Koch, Dakota T. Thompson, Dana M. Van der Heide, Jeremy Chang, Christopher M. Franke, Mohammed O. Suraju, Anna C. Beck, Allison W. Lorenzen, Jeffrey R. White, Nicholas I. Bartschat, Mikhail V. Kulak, David K. Meyerholz, Colin Kenny, Ronald J. Weigel

{"title":"Sumoylated Etv1 establishes mouse mammary cancer stem cells that support tumorigenesis by non-stem cancer cells","authors":"Zhijie Li, Kelsey E. Koch, Dakota T. Thompson, Dana M. Van der Heide, Jeremy Chang, Christopher M. Franke, Mohammed O. Suraju, Anna C. Beck, Allison W. Lorenzen, Jeffrey R. White, Nicholas I. Bartschat, Mikhail V. Kulak, David K. Meyerholz, Colin Kenny, Ronald J. Weigel","doi":"10.1016/j.devcel.2025.04.005","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.005","url":null,"abstract":"The small ubiquitin-like modifier (SUMO) pathway is required for maintenance of cancer stem cells/tumor-initiating cells (CSCs/TICs), which drive tumorigenesis when transplanted into immunocompromised mice. We found that inhibition of the SUMO pathway blocked Neu-mediated mammary oncogenesis and inhibited the function of CSCs/TICs without effects on normal mammary stem cells. Transcriptomic analysis implicated SUMO-conjugated Etv1 as being critical for oncogenesis. After SUMO pathway inhibition, a SUMO-mimetic Etv1 protein, created by a fusion with SUMO1 or SUMO2, established a stem-like cell capable of tumorigenesis, whereas a SUMO-resistant Etv1 protein established a proliferative, non-tumorigenic cell. In mixing experiments, stem-like cells induced tumorigenesis by non-stem cells. We conclude that SUMO-conjugated Etv1 is necessary to maintain the CSC/TIC phenotype and that crosstalk between stem and non-stem cells is crucial for tumorigenesis. The findings demonstrate dynamic interactions between heterogeneous cell types to drive tumorigenesis, which has implications for future cancer therapeutic development.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"11 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143893219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteria suppress immune responses in Arabidopsis by inducing methylglyoxal accumulation and promoting H2O2 scavenging 细菌通过诱导甲基乙二醛积累和促进H2O2清除来抑制拟南芥的免疫反应

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-29 DOI: 10.1016/j.devcel.2025.04.006

Ting-Ting Yuan, Yu-Rui Feng, Hua Cheng, Shuiyuan Cheng, Ying-Tang Lu

引用次数: 0

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation 线粒体CCN1通过脂肪酸β氧化驱动铁下垂

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-24 DOI: 10.1016/j.devcel.2025.04.004

Wanxin Guo, Congcong Zhang, Qianjun Zhou, Tianxiang Chen, Xin Xu, Jianfeng Zhang, Xuewen Yu, Han Wu, Xiao Zhang, Lifang Ma, Kun Qian, Daniel J. Klionsky, Rui Kang, Guido Kroemer, Yongchun Yu, Daolin Tang, Jiayi Wang

{"title":"Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation","authors":"Wanxin Guo, Congcong Zhang, Qianjun Zhou, Tianxiang Chen, Xin Xu, Jianfeng Zhang, Xuewen Yu, Han Wu, Xiao Zhang, Lifang Ma, Kun Qian, Daniel J. Klionsky, Rui Kang, Guido Kroemer, Yongchun Yu, Daolin Tang, Jiayi Wang","doi":"10.1016/j.devcel.2025.04.004","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.004","url":null,"abstract":"Ferroptosis is a type of oxidative cell death, although its key metabolic processes remain incompletely understood. Here, we employ a comprehensive multiomics screening approach that identified cellular communication network factor 1 (CCN1) as a metabolic catalyst of ferroptosis. Upon ferroptosis induction, CCN1 relocates to mitochondrial complexes, facilitating electron transfer flavoprotein subunit alpha (ETFA)-dependent fatty acid β-oxidation. Compared with a traditional carnitine O-palmitoyltransferase 2 (CPT2)-ETFA pathway, the CCN1-ETFA pathway provides additional substrates for mitochondrial reactive oxygen species production, thereby stimulating ferroptosis through lipid peroxidation. A high-fat diet can enhance the anticancer efficacy of ferroptosis in lung cancer mouse models, depending on CCN1. Furthermore, primary lung cancer cells derived from patients with hypertriglyceridemia or high CCN1 expression demonstrate increased susceptibility to ferroptosis in vitro and in vivo. These findings do not only identify the metabolic role of mitochondrial CCN1 but also establish a strategy for enhancing ferroptosis-based anticancer therapies.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"219 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic control of H2A.Zub and H3K27me3 by ambient temperature during cell fate determination in Arabidopsis H2A的动态控制。Zub和H3K27me3在拟南芥细胞命运决定中的作用

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-22 DOI: 10.1016/j.devcel.2025.04.002

Kehui Zhu, Jinchao Chen, Long Zhao, Fangfang Lu, Jia Deng, Xuelei Lin, Chongsheng He, Doris Wagner, Jun Xiao

{"title":"Dynamic control of H2A.Zub and H3K27me3 by ambient temperature during cell fate determination in Arabidopsis","authors":"Kehui Zhu, Jinchao Chen, Long Zhao, Fangfang Lu, Jia Deng, Xuelei Lin, Chongsheng He, Doris Wagner, Jun Xiao","doi":"10.1016/j.devcel.2025.04.002","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.002","url":null,"abstract":"Crucial to plant development, ambient temperature triggers intricate mechanisms enabling adaptive responses to temperature variations. The precise coordination of chromatin modifications in shaping cell developmental fate under diverse temperatures remains elusive. Our study, integrating comprehensive transcriptome, epigenome profiling, and genetics, demonstrates that lower ambient temperature (16°C) partially restores developmental defects caused by H3K27me3 loss in prc2 mutants by specifically depositing H2A.Zub at ectopically expressed embryonic genes in Arabidopsis, such as ABA INSENSITIVE 3 (ABI3) and LEAFY COTYLEDON 1 (LEC1). This deposition leads to downregulation of these genes and compensates for H3K27me3 depletion. Polycomb-repressive complex 1 (PRC1)-catalyzed H2A.Zub and PRC2-catalyzed H3K27me3 play roles in silencing transcription of embryonic genes for post-germination development. Low-temperature-induced reduction of TOE1 protein level decelerates H2A.Z turnover at specific loci, sustaining repression of embryonic genes and alleviating requirement for PRC2-H3K27me3 at post-germination stage. Our findings offer mechanistic insights into the cooperative epigenetic layers, facilitating plant adaptation to varying environmental temperatures.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"29 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide controls stomatal development and stress responses by inhibiting MPK6 phosphorylation via S-nitrosylation in Arabidopsis 在拟南芥中，一氧化氮通过s -亚硝基化抑制MPK6磷酸化来控制气孔发育和胁迫反应

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-21 DOI: 10.1016/j.devcel.2025.04.001

Danfeng Wang, Hongyan Guo, Xinru Gong, Lichao Chen, Huifang Lin, Shiping Wang, Tianpeng Feng, Yanyan Yi, Wan Wang, Shuhua Yang, Jie Le, Lixin Zhang, Jianru Zuo

{"title":"Nitric oxide controls stomatal development and stress responses by inhibiting MPK6 phosphorylation via S-nitrosylation in Arabidopsis","authors":"Danfeng Wang, Hongyan Guo, Xinru Gong, Lichao Chen, Huifang Lin, Shiping Wang, Tianpeng Feng, Yanyan Yi, Wan Wang, Shuhua Yang, Jie Le, Lixin Zhang, Jianru Zuo","doi":"10.1016/j.devcel.2025.04.001","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.04.001","url":null,"abstract":"In plants, stomata on the aerial epidermis play critical roles in various biological processes, including gas exchange, photosynthesis, transpiration, and immunity. Stomatal development is negatively and positively controlled by the mitogen-activated protein kinase (MAPK) cascade and nitric oxide (NO), respectively. However, the regulatory scheme of stomatal development by these signaling pathways remains elusive. Here, we show that NO-controlled stomatal development in Arabidopsis is genetically dependent on MPK3 and MPK6. Moreover, NO-controlled S-nitrosylation of MPK6 at cysteine (Cys)-201 inhibits its phosphorylation, resulting in the stabilization of SPEECHLESS (SPCH), a master regulator of stomatal lineage initiation, thereby promoting stomatal development. An MPK6C201S mutation confers NO insensitivity during stomatal development and stress responses. We propose that NO positively controls stomatal development and stress responses by inhibiting the MPK6 activity via S-nitrosylation, thus identifying a mechanism linking the coupled NO-MAPK signaling to specific biological outputs.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"56 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling 表达bmi1的小鼠肠道干细胞的双重状态利用非规范Wnt信号驱动上皮发育

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-04-21 DOI: 10.1016/j.devcel.2025.03.014

Nicholas R. Smith, Nicole R. Giske, Sidharth K. Sengupta, Patrick Conley, John R. Swain, Ashvin Nair, Kathryn L. Fowler, Christopher Klocke, Yeon Jung Yoo, Ashley N. Anderson, Nasim Sanati, Kristof Torkenczy, Andrew C. Adey, Jared M. Fischer, Guanming Wu, Melissa H. Wong

{"title":"Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling","authors":"Nicholas R. Smith, Nicole R. Giske, Sidharth K. Sengupta, Patrick Conley, John R. Swain, Ashvin Nair, Kathryn L. Fowler, Christopher Klocke, Yeon Jung Yoo, Ashley N. Anderson, Nasim Sanati, Kristof Torkenczy, Andrew C. Adey, Jared M. Fischer, Guanming Wu, Melissa H. Wong","doi":"10.1016/j.devcel.2025.03.014","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.03.014","url":null,"abstract":"Intestinal epithelial development and homeostasis critically rely upon balanced stem cell proliferation, involving slow-cycling/label-retaining and active-cycling/canonical Wnt-dependent intestinal stem cell (ISC) subtypes. ISC regulation during development remains poorly understood but has important implications for establishing key mechanisms governing tissue maintenance. Herein, we identify Bmi1+ cells as functional stem cells present in early murine intestinal development, prior to Lgr5-expressing ISCs. Lineage tracing and single-cell RNA sequencing identify that Bmi1+ ISCs can trace to Lgr5+ ISCs and other differentiated lineages. Initially highly proliferative, Bmi1+ ISCs transition to slow-cycling states as Lgr5+ ISCs emerge. Non-canonical Wnt signaling regulates the proliferative Bmi1+ cell state. These findings highlight the dynamic interplay between stem cell populations and the opposing Wnt pathways that govern proliferation—ultimately having implications for tissue development, homeostasis, regeneration, and tumorigenesis. Understanding these fundamental developmental mechanisms is critical for understanding adult intestinal maintenance.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"10 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0