Developmental cell最新文献_第2页

Lysosomal catabolic activity promotes the exit of murine totipotent 2-cell state by silencing early-embryonic retrotransposons 溶酶体分解代谢活动通过沉默早期胚胎逆转录载体促进小鼠脱离全能2细胞状态

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.018

Hao Wu, Lanrui Cao, Xinpeng Wen, Jiawei Fan, Yuan Wang, Heyong Hu, Shuyan Ji, Yinli Zhang, Cunqi Ye, Wei Xie, Jin Zhang, Haoxing Xu, Xudong Fu

{"title":"Lysosomal catabolic activity promotes the exit of murine totipotent 2-cell state by silencing early-embryonic retrotransposons","authors":"Hao Wu, Lanrui Cao, Xinpeng Wen, Jiawei Fan, Yuan Wang, Heyong Hu, Shuyan Ji, Yinli Zhang, Cunqi Ye, Wei Xie, Jin Zhang, Haoxing Xu, Xudong Fu","doi":"10.1016/j.devcel.2024.10.018","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.018","url":null,"abstract":"During mouse preimplantation development, a subset of retrotransposons/genes are transiently expressed in the totipotent 2-cell (2C) embryos. These 2C transcripts rapidly shut down their expression beyond the 2C stage of embryos, promoting the embryo to exit from the 2C stage. However, the mechanisms regulating this shutdown remain unclear. Here, we identified that lysosomal catabolism played a role in the exit of the totipotent 2C state. Our results showed that the activation of embryonic lysosomal catabolism promoted the embryo to exit from the 2C stage and suppressed 2C transcript expression. Mechanistically, our results indicated that lysosomal catabolism suppressed 2C transcripts through replenishing cellular amino-acid levels, thereby inactivating transcriptional factors TFE3/TFEB and abolishing their transcriptional activation of 2C retrotransposons, MERVL (murine endogenous retrovirus-L)/MT2_Mm. Collectively, our study identified that lysosomal activity modulated the transcriptomic landscape and development in mouse embryos and identified an unanticipated layer of transcriptional control on early-embryonic retrotransposons from lysosomal signaling.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"34 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a non-canonical planar cell polarity pathway triggered by light in the developing mouse retina 在发育中的小鼠视网膜中发现由光引发的非经典平面细胞极性通路

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.012

Michael Housset, Dominic Filion, Nelson Cortes, Hojatollah Vali, Craig A. Mandato, Christian Casanova, Michel Cayouette

引用次数: 0

Initiation and maintenance of the pluripotent epiblast in pre-implantation human development is independent of NODAL signaling 植入前人类发育过程中多能上胚层的启动和维持与 NODAL 信号无关

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-18 DOI: 10.1016/j.devcel.2024.10.020

A. Sophie Brumm, Afshan McCarthy, Claudia Gerri, Todd Fallesen, Laura Woods, Riley McMahon, Athanasios Papathanasiou, Kay Elder, Phil Snell, Leila Christie, Patricia Garcia, Valerie Shaikly, Mohamed Taranissi, Paul Serhal, Rabi A. Odia, Mina Vasilic, Anna Osnato, Peter J. Rugg-Gunn, Ludovic Vallier, Caroline S. Hill, Kathy K. Niakan

{"title":"Initiation and maintenance of the pluripotent epiblast in pre-implantation human development is independent of NODAL signaling","authors":"A. Sophie Brumm, Afshan McCarthy, Claudia Gerri, Todd Fallesen, Laura Woods, Riley McMahon, Athanasios Papathanasiou, Kay Elder, Phil Snell, Leila Christie, Patricia Garcia, Valerie Shaikly, Mohamed Taranissi, Paul Serhal, Rabi A. Odia, Mina Vasilic, Anna Osnato, Peter J. Rugg-Gunn, Ludovic Vallier, Caroline S. Hill, Kathy K. Niakan","doi":"10.1016/j.devcel.2024.10.020","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.020","url":null,"abstract":"The human blastocyst contains the pluripotent epiblast from which human embryonic stem cells (hESCs) can be derived. ACTIVIN/NODAL signaling maintains expression of the transcription factor NANOG and in vitro propagation of hESCs. It is unknown whether this reflects a functional requirement for epiblast development in human embryos. Here, we characterized NODAL signaling activity during pre-implantation human development. We showed that NANOG is an early molecular marker restricted to the nascent human pluripotent epiblast and was initiated prior to the onset of NODAL signaling. We further demonstrated that expression of pluripotency-associated transcription factors NANOG, SOX2, OCT4, and KLF17 were maintained in the epiblast in the absence of NODAL signaling activity. Genome-wide transcriptional analysis showed that NODAL signaling inhibition did not decrease NANOG transcription or impact the wider pluripotency-associated gene regulatory network. These data suggest differences in the signaling requirements regulating pluripotency in the pre-implantation human epiblast compared with existing hESC culture.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"76 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biased cell adhesion organizes the Drosophila visual motion integration circuit 偏向细胞粘附组织果蝇视觉运动整合回路

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-15 DOI: 10.1016/j.devcel.2024.10.019

Yannick Carrier, Laura Quintana Rio, Nadia Formicola, Vicente de Sousa-Xavier, Maha Tabet, Yu-Chieh David Chen, Aicha Haji Ali, Maëva Wislez, Lisa Orts, Alexander Borst, Filipe Pinto-Teixeira

{"title":"Biased cell adhesion organizes the Drosophila visual motion integration circuit","authors":"Yannick Carrier, Laura Quintana Rio, Nadia Formicola, Vicente de Sousa-Xavier, Maha Tabet, Yu-Chieh David Chen, Aicha Haji Ali, Maëva Wislez, Lisa Orts, Alexander Borst, Filipe Pinto-Teixeira","doi":"10.1016/j.devcel.2024.10.019","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.019","url":null,"abstract":"Layer-specific brain computations depend on neurons synapsing with specific partners in distinct laminae. In the Drosophila lobula plate, axons of the four subtypes of T4 and T5 visual motion direction-selective neurons segregate into four layers, where they synapse with distinct subsets of postsynaptic neurons. Here, we identify a layer-specific expression of different receptor-ligand pairs of the Beat and Side families of cell adhesion molecules between T4/T5s and their postsynaptic partners. Developmental genetic analysis demonstrate that Beat/Side-mediated interactions are required to restrict innervation of T4/T5 axons and the dendrites of their partners to a single layer. We show that Beat/Side interactions are not required for synaptogenesis. Instead, they contribute to synaptic specificity by biasing cellular adjacency, causing neurons to segregate in discrete layers, restricting partner availability before synaptogenesis. We propose that the emergence of synaptic specificity relies on a competitive dynamic among postsynaptic partners with shared Beat/Side expression to adhere with T4/T5s.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"1 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunneling nanotubes enable intercellular transfer in zebrafish embryos 隧道纳米管实现斑马鱼胚胎细胞间转移

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-13 DOI: 10.1016/j.devcel.2024.10.016

Olga Korenkova, Shiyu Liu, Inès Prlesi, Anna Pepe, Shahad Albadri, Filippo Del Bene, Chiara Zurzolo

{"title":"Tunneling nanotubes enable intercellular transfer in zebrafish embryos","authors":"Olga Korenkova, Shiyu Liu, Inès Prlesi, Anna Pepe, Shahad Albadri, Filippo Del Bene, Chiara Zurzolo","doi":"10.1016/j.devcel.2024.10.016","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.016","url":null,"abstract":"Tunneling nanotubes (TNTs) are thin intercellular connections that facilitate the transport of diverse cargoes, ranging from ions to organelles. While TNT studies have predominantly been conducted in cell cultures, the existence of open-ended TNTs within live organisms remains unverified. Despite the observation of intercellular connections during embryonic development across various species, their functional role in facilitating material transfer between connected cells has not been confirmed. In this study, we performed mosaic labeling of gastrula cells in zebrafish embryos to demonstrate the coexistence of TNT-like structures alongside other cellular protrusions. These embryonic TNT-like connections exhibited a morphology similar to that of TNTs described in cell culture, appeared to have similar formation mechanisms, and could be induced by Eps8 overexpression and CK666 treatment. Most notably, we demonstrated their capability to transfer both soluble cargoes and organelles, thus confirming their open-endedness. This study demonstrates the existence of functional, open-ended TNTs in a living embryo.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"8 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary innovations in germline biology of placental mammals identified by transcriptomics of first-wave spermatogenesis in opossum 通过对负鼠第一波精子发生的转录组学研究发现胎盘哺乳动物生殖系生物学的进化创新

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-12 DOI: 10.1016/j.devcel.2024.10.013

Kira L. Marshall, Daniel J. Stadtmauer, Jamie Maziarz, Günter P. Wagner, Bluma J. Lesch

引用次数: 0

Dephosphorylation of bZIP59 by PP2A ensures appropriate shade avoidance response in Arabidopsis PP2A 对 bZIP59 的去磷酸化可确保拟南芥做出适当的避阴反应

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-12 DOI: 10.1016/j.devcel.2024.10.014

Fengquan Li, Jiayu Wang, Pengcheng Wang, Lin Li

{"title":"Dephosphorylation of bZIP59 by PP2A ensures appropriate shade avoidance response in Arabidopsis","authors":"Fengquan Li, Jiayu Wang, Pengcheng Wang, Lin Li","doi":"10.1016/j.devcel.2024.10.014","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.014","url":null,"abstract":"Changes in light quality and quantity experienced by many shade-intolerant plants grown in close proximity lead to transcriptional reprogramming and shade avoidance syndrome (SAS). Despite the importance of phosphorylation-dependent signaling in cellular physiology, phosphorylation events during SAS are largely unknown. Here, we examined shade-regulated phosphorylation events in Arabidopsis using quantitative phosphoproteomics. We confirmed shade-induced dephosphorylation of bZIP59, a basic region/leucine zipper motif (bZIP) transcription factor. Shade treatment promotes the nuclear localization of bZIP59, which can be mimicked by mutation of the phosphorylation sites on bZIP59. Phenotypic analysis identified that bZIP59 negatively regulated shade-induced hypocotyl elongation. bZIP59 repressed the shade-induced activation of certain growth-related genes, while shade increased the DNA binding of bZIP59. Furthermore, the protein phosphatase 2A (PP2A) mediated dephosphorylation of bZIP59. Our study characterized a previously unidentified mechanism by which the phytochrome B (phyB)-PP2A-bZIP59 regulatory module integrates shade signals and transcriptomes, broadening our knowledge of phosphorylation strategies for rapid adaptation to shade.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"95 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Drosophila adult midgut progenitor cells arise from asymmetric divisions of neuroblast-like cells 果蝇成体中肠祖细胞来自神经母细胞的不对称分裂

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-11 DOI: 10.1016/j.devcel.2024.10.011

Andrew T. Plygawko, Camille Stephan-Otto Attolini, Ioanna Pitsidianaki, David P. Cook, Alistair C. Darby, Kyra Campbell

引用次数: 0

Systematic screening of enhancer-blocking insulators in Drosophila identifies their DNA sequence determinants 系统筛选果蝇的增强子阻断绝缘子，确定其 DNA 序列决定因素

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-11 DOI: 10.1016/j.devcel.2024.10.017

Anastasiia Tonelli, Pascal Cousin, Aleksander Jankowski, Bihan Wang, Julien Dorier, Jonas Barraud, Sanyami Zunjarrao, Maria Cristina Gambetta

{"title":"Systematic screening of enhancer-blocking insulators in Drosophila identifies their DNA sequence determinants","authors":"Anastasiia Tonelli, Pascal Cousin, Aleksander Jankowski, Bihan Wang, Julien Dorier, Jonas Barraud, Sanyami Zunjarrao, Maria Cristina Gambetta","doi":"10.1016/j.devcel.2024.10.017","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.017","url":null,"abstract":"Long-range transcriptional activation of gene promoters by abundant enhancers in animal genomes calls for mechanisms to limit inappropriate regulation. DNA elements called insulators serve this purpose by shielding promoters from an enhancer when interposed. Unlike promoters and enhancers, insulators have not been systematically characterized due to lacking high-throughput screening assays, and questions regarding how insulators are distributed and encoded in the genome remain. Here, we establish “insulator-seq” as a plasmid-based massively parallel reporter assay in Drosophila cultured cells to perform a systematic insulator screen of selected genomic loci. Screening developmental gene loci showed that not all insulator protein binding sites effectively block enhancer-promoter communication. Deep insulator mutagenesis identified sequences flexibly positioned around the CTCF insulator protein binding motif that are critical for functionality. The ability to screen millions of DNA sequences without positional effect has enabled functional mapping of insulators and provided further insights into the determinants of insulators.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"19 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FoxA1/2-dependent epigenomic reprogramming drives lineage switching in lung adenocarcinoma FoxA1/2 依赖性表观基因组重编程驱动肺腺癌的系谱转换

IF 11.8 1区生物学

Developmental cell Pub Date : 2024-11-07 DOI: 10.1016/j.devcel.2024.10.009

Katherine Gillis, Walter A. Orellana, Emily Wilson, Timothy J. Parnell, Gabriela Fort, Pengshu Fang, Headtlove Essel Dadzie, Brandon M. Murphy, Xiaoyang Zhang, Eric L. Snyder

{"title":"FoxA1/2-dependent epigenomic reprogramming drives lineage switching in lung adenocarcinoma","authors":"Katherine Gillis, Walter A. Orellana, Emily Wilson, Timothy J. Parnell, Gabriela Fort, Pengshu Fang, Headtlove Essel Dadzie, Brandon M. Murphy, Xiaoyang Zhang, Eric L. Snyder","doi":"10.1016/j.devcel.2024.10.009","DOIUrl":"https://doi.org/10.1016/j.devcel.2024.10.009","url":null,"abstract":"The ability of cancer cells to undergo identity changes (i.e., lineage plasticity) plays a key role in tumor progression and response to therapy. Loss of the pulmonary lineage specifier NKX2-1 in KRAS-driven lung adenocarcinoma (LUAD) enhances tumor progression and causes a FoxA1/2-dependent pulmonary-to-gastric lineage switch. However, the mechanisms by which FoxA1/2 activate a latent gastric identity in the lung remain largely unknown. Here, we show that FoxA1/2 reprogram the epigenetic landscape of gastric-specific genes after NKX2-1 loss in mouse models by facilitating ten-eleven translocation (TET)2/3 recruitment, DNA demethylation, histone 3 lysine 27 acetylation (H3K27ac) deposition, and three-dimensional (3D) chromatin interactions. FoxA1/2-mediated DNA methylation changes are highly conserved in human endodermal development and in progression of human lung and pancreatic neoplasia. Furthermore, oncogenic signaling is required for specific elements of FoxA1/2-dependent epigenetic reprogramming. This work demonstrates the role of FoxA1/2 in rewiring the DNA methylation and 3D chromatin landscape of NKX2-1-negative LUAD to drive cancer cell lineage switching.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"3 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0