Developmental cell最新文献_第3页

Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation 母体CENP-C恢复哺乳动物受精卵的着丝粒对称性，以确保适当的染色体分离

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-24 DOI: 10.1016/j.devcel.2025.08.017

Catherine A. Tower, Gabriel Manske, Emily L. Ferrell, Dilara N. Anbarci, Kelsey Jorgensen, Binbin Ma, Mansour Aboelenain, Rajesh Ranjan, Saikat Chakraborty, Lindsay Moritz, Arunika Das, Michele Boiani, Ben E. Black, Shawn Chavez, Erica E. Marsh, Ariella Shikanov, Karen Schindler, Xin Chen, Saher Sue Hammoud

{"title":"Maternal CENP-C restores centromere symmetry in mammalian zygotes to ensure proper chromosome segregation","authors":"Catherine A. Tower, Gabriel Manske, Emily L. Ferrell, Dilara N. Anbarci, Kelsey Jorgensen, Binbin Ma, Mansour Aboelenain, Rajesh Ranjan, Saikat Chakraborty, Lindsay Moritz, Arunika Das, Michele Boiani, Ben E. Black, Shawn Chavez, Erica E. Marsh, Ariella Shikanov, Karen Schindler, Xin Chen, Saher Sue Hammoud","doi":"10.1016/j.devcel.2025.08.017","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.017","url":null,"abstract":"Across metazoan species, the centromere-specific histone variant CENP-A is essential for accurate chromosome segregation, yet its regulation during the mammalian parental-to-zygote transition is poorly understood. To address this, we generated a CENP-A-mScarlet mouse model that revealed sex-specific dynamics: mature sperm retain 10% of the CENP-A levels present in MII oocytes. However, this difference is resolved in zygotes prior to the first mitosis, using maternally inherited cytoplasmic CENP-A. Notably, the increase in CENP-A at paternal centromeres is independent of sensing CENP-A asymmetry or the presence of maternal chromosomes. Instead, CENP-A equalization relies on the asymmetric recruitment of maternal CENP-C to paternal centromeres. Depletion of maternal CENP-A decreases total CENP-A in both pronuclei without disrupting equalization. In contrast, reducing maternal CENP-C or disruption of its dimerization function impairs CENP-A equalization and chromosome segregation. Therefore, maternal CENP-C acts as a key epigenetic regulator that resets centromeric symmetry at fertilization to preserve genome integrity.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"11 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145127337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascularizing stem cell-derived islets: A blueprint for functional maturation 血管干细胞衍生的胰岛：功能成熟的蓝图

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-22 DOI: 10.1016/j.devcel.2025.07.012

Yan Xiong, Per-Olof Berggren

引用次数: 0

Enhancers, silencers, and attenuators: A dynamic and reversible regulatory code 增强器、消声器和衰减器：一种动态可逆的调节代码

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-22 DOI: 10.1016/j.devcel.2025.06.039

Emily R. Wilson, Xiaoyang Zhang

引用次数: 0

Emerging insights into lineage plasticity in pancreatic cancer initiation, progression, and therapy resistance 胰腺癌起始、进展和治疗耐药谱系可塑性的新见解

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-22 DOI: 10.1016/j.devcel.2025.07.002

Xintong Zhang, Yaru Du, Axel Behrens, Linxiang Lan

{"title":"Emerging insights into lineage plasticity in pancreatic cancer initiation, progression, and therapy resistance","authors":"Xintong Zhang, Yaru Du, Axel Behrens, Linxiang Lan","doi":"10.1016/j.devcel.2025.07.002","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.07.002","url":null,"abstract":"Lineage plasticity, the ability of cells to switch from one specialized identity to another, is a fundamental cellular process in embryonic development and tissue regeneration. The process is often hijacked by tumor cells at various stages to facilitate cancer initiation, progression, metastasis, and therapy resistance. It is also recognized as a key contributor to intratumor heterogeneity (ITH). In pancreatic ductal adenocarcinoma (PDAC), lineage plasticity is central to acinar-to-ductal metaplasia (ADM) and its associated acinar-to-ductal reprogramming (ADR), epithelial-mesenchymal transition (EMT), cancer stem cell (CSC) regeneration, and molecular subtype rewiring. These mechanisms generate diverse lineage trajectories that shape PDAC development, progression, and therapeutic outcomes. In this review, we discuss how normal and tumor cells in the pancreatic epithelium acquire lineage plasticity and its implications for PDAC pathogenesis, ITH, metastasis, and therapy resistance. We also highlight recent discoveries suggesting potential therapeutic strategies targeting key regulators of lineage plasticity in PDAC.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"14 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Living on the edge: Uncommitted OPC-like cells drive glioblastoma invasiveness 生活在边缘：未承诺的opc样细胞驱动胶质母细胞瘤侵袭性

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-22 DOI: 10.1016/j.devcel.2025.08.002

Upendra K. Soni, Q. Richard Lu

引用次数: 0

Signaling-dependent refinement of cell fate choice during tissue remodeling in Drosophila pupal wings 果蝇蛹翅组织重塑过程中细胞命运选择的信号依赖细化

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-18 DOI: 10.1016/j.devcel.2025.08.016

Sophie Herszterg, Simone Cicolini, Marc de Gennes, Anqi Huang, Alexis Matamoro-Vidal, Cyrille Alexandre, Matthew Smith, Helena Araujo, Romain Levayer, Jean-Paul Vincent, Guillaume Salbreux

{"title":"Signaling-dependent refinement of cell fate choice during tissue remodeling in Drosophila pupal wings","authors":"Sophie Herszterg, Simone Cicolini, Marc de Gennes, Anqi Huang, Alexis Matamoro-Vidal, Cyrille Alexandre, Matthew Smith, Helena Araujo, Romain Levayer, Jean-Paul Vincent, Guillaume Salbreux","doi":"10.1016/j.devcel.2025.08.016","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.016","url":null,"abstract":"How cell fate decisions and tissue remodeling are coordinated to establish precise and robust patterns is a fundamental question in developmental biology. Here, we investigate this interplay during the refinement of Drosophila wing veins. We show by live imaging that vein refinement is driven initially by local tissue deformation, followed by cell fate adjustments orchestrated by a signaling network involving Notch, EGFR, and Dpp. Dynamic tracking of signaling reporter activity uncovers a wave of Notch signaling that converts wide crude proveins into thin stereotypical veins. Perturbing large-scale convergence and extension does not affect vein refinement, and optogenetically induced veins refine irrespective of their orientation, demonstrating that the signaling network suffices for refinement, independently of large-scale tissue flows. A minimal biophysical description recapitulates the signaling network’s ability to coordinate vein refinement in various experimental situations. Our results illustrate how cell fate decisions are updated for robust patterning in a remodeling tissue.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"16 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomics-based multi-omics identifies the roadmap of transcription-translation-protein dynamics in planarian regeneration 基于蛋白质组学的多组学确定了涡虫再生中转录-翻译-蛋白质动力学的路线图

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-16 DOI: 10.1016/j.devcel.2025.08.015

Yuqing Ying, Yuanyi Zhou Xiong, Xue Pan, Qiushi Zhang, Jiajia Chen, Yun Zhao, Xue Cai, Xiao Yi, Yi Zhu, Tiannan Guo, Kai Lei

{"title":"Proteomics-based multi-omics identifies the roadmap of transcription-translation-protein dynamics in planarian regeneration","authors":"Yuqing Ying, Yuanyi Zhou Xiong, Xue Pan, Qiushi Zhang, Jiajia Chen, Yun Zhao, Xue Cai, Xiao Yi, Yi Zhu, Tiannan Guo, Kai Lei","doi":"10.1016/j.devcel.2025.08.015","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.015","url":null,"abstract":"Identifying regulators for tissue regeneration is fundamental for regenerative biology. While transcription dynamics control planarian regeneration initiation, how protein machinery controls regeneration remains unclear, as transcript levels often fail to predict protein abundance. To address this gap, we performed mass-spectrometry-based proteomic analyses of the planarian Schmidtea mediterranea, establishing a spectral library covering ∼10,000 proteins, and employed quantitative approaches to measure proteome dynamics during regeneration. Our study identified upregulated ribosomal proteins, which were supported by ribosome profiling sequencing (Ribo-seq). Combining RNA sequencing (RNA-seq) and Ribo-seq analyses categorized the increased protein abundance into regulatory modes at transcriptional, translational, and protein stability levels. Functional examination identified 25 proteins essential for planarian regeneration. Troponin T was identified as a regulator of regeneration initiation, showing increased protein abundance before upregulation at transcriptional and translational levels, suggesting a regulation of protein stability. In summary, our study demonstrates previously unexplored ribosome-mediated and transcription-independent protein machinery essential for planarian regeneration initiation.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"72 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145067881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomolecular condensates of ATG18 reshape ER for autophagy in plants ATG18的生物分子凝聚物重塑内质网，促进植物自噬

IF 11.8 1区生物学

Developmental cell Pub Date : 2025-09-11 DOI: 10.1016/j.devcel.2025.08.013

Yang Shao, Xunzheng Li, Benhui Shi, Songyang Wang, Zisheng Luo, Yanqun Xu, Baolei Li, Shuqing Feng, Li Liang, Huanquan Zheng, Jiaqi Sun

{"title":"Biomolecular condensates of ATG18 reshape ER for autophagy in plants","authors":"Yang Shao, Xunzheng Li, Benhui Shi, Songyang Wang, Zisheng Luo, Yanqun Xu, Baolei Li, Shuqing Feng, Li Liang, Huanquan Zheng, Jiaqi Sun","doi":"10.1016/j.devcel.2025.08.013","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.013","url":null,"abstract":"Autophagosomes originate from and maintain association with the endoplasmic reticulum (ER) during their formation, yet how these processes are molecularly coordinated in plants remains poorly understood. Here, we demonstrate that Arabidopsis autophagy-related protein 18a (ATG18a), a key organizer of early autophagosome formation, undergoes phase separation to form biomolecular condensates on the ER membrane, which progress from highly mobile droplets to stable ring-like structures, while the ER is reshaped. We discovered that ATG18a condensates work together with ROOT HAIR DEFECTIVE3 (RHD3), an ER membrane-shaping protein, with RABC1 serving as a molecular linker between them. Importantly, RABC1 facilitates both RHD3 assembly necessary for the formation of ring-like ER structures and its interaction with ATG18a condensates. These findings reveal a mechanism whereby biomolecular condensates work together with membrane-shaping proteins to reshape specialized membrane domains through wetting interactions, providing an insight into autophagosome formation in plant stress responses.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":"312 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145043287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early microglia progenitors colonize the embryonic CNS via integrin-mediated migration from the pial surface. 早期的小胶质细胞祖细胞通过整合素介导的从头部表面迁移到胚胎中枢神经系统。

IF 8.7 1区生物学

Developmental cell Pub Date : 2025-09-10 DOI: 10.1016/j.devcel.2025.08.012

Philippe Petry, Alexander Oschwald, Simon Merkt, Thien-Ly Julia Dinh, Geoffroy Andrieux, Cylia Crisand, Hannah Botterer, Elisa Nent, Neil Paterson, Monique Havermans, Roman Sankowski, Oliver Schilling, Melanie Boerries, Lukas Amann, Olaf Groß, Andreas Schlitzer, Marco Prinz, Tim Lämmermann, Katrin Kierdorf

{"title":"Early microglia progenitors colonize the embryonic CNS via integrin-mediated migration from the pial surface.","authors":"Philippe Petry, Alexander Oschwald, Simon Merkt, Thien-Ly Julia Dinh, Geoffroy Andrieux, Cylia Crisand, Hannah Botterer, Elisa Nent, Neil Paterson, Monique Havermans, Roman Sankowski, Oliver Schilling, Melanie Boerries, Lukas Amann, Olaf Groß, Andreas Schlitzer, Marco Prinz, Tim Lämmermann, Katrin Kierdorf","doi":"10.1016/j.devcel.2025.08.012","DOIUrl":"https://doi.org/10.1016/j.devcel.2025.08.012","url":null,"abstract":"Macrophage progenitors colonize their anatomical niches in the central nervous system (CNS) in distinct pre- and postnatal waves. Microglia progenitors originate from early erythromyeloid progenitors in the yolk sac and enter the murine CNS around embryonic day (E)9.5. While their developmental origin is well established, the molecular mechanisms guiding CNS colonization are not yet resolved. Using transcriptomic and proteomic approaches, we identified potential factors involved in this process. Microglia progenitors showed a distinct integrin surface profile and transmigrate along the extracellular matrix (ECM)-enriched pial surface into the CNS, pointing to a mesenchyme-to-CNS migration route. Loss of the integrin adaptor protein talin-1 in microglia progenitors led to a reduced CNS colonization, whereas macrophage progenitors in the surrounding mesenchyme remained unchanged. Overall, our data suggest that microglial progenitors enter the CNS parenchyma via talin-1-mediated migration from the surrounding mesenchyme through the ECM-enriched pial surface.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental and genetic risk factors of depression converge on neuronal dysfunction driven by changes in cholesterol homeostasis. 抑郁症的环境和遗传风险因素集中在由胆固醇稳态变化驱动的神经元功能障碍上。

IF 8.7 1区生物学

Developmental cell Pub Date : 2025-09-10 DOI: 10.1016/j.devcel.2025.08.011

Polina Oberst, Nan Xu, Hermany Munguba, Chao Zhang, Aaron Zhong, Ting Zhou, Conor Liston, Joshua Levitz, Lorenz Studer

{"title":"Environmental and genetic risk factors of depression converge on neuronal dysfunction driven by changes in cholesterol homeostasis.","authors":"Polina Oberst, Nan Xu, Hermany Munguba, Chao Zhang, Aaron Zhong, Ting Zhou, Conor Liston, Joshua Levitz, Lorenz Studer","doi":"10.1016/j.devcel.2025.08.011","DOIUrl":"10.1016/j.devcel.2025.08.011","url":null,"abstract":"Major depressive disorder (MDD) is a complex, multifactorial neuropsychiatric disorder influenced by both genetic and environmental factors, but how these factors impact human neuronal function remains unclear. Using a highly defined human pluripotent stem cell (hPSC)-based prefrontal cortex (PFC) platform, we examined three high-confidence environmental and genetic factors associated with depression: chronic exposure to high levels of cortisol or interferon alpha (IFN-a), and a mutation in SIRTUIN 1 (SIRT1). All three conditions induced overlapping phenotypes of neuronal dysfunction, characterized by dendritic atrophy, synaptic loss, and neuronal hypoactivity across multiple cell lines. RNA sequencing uncovered converging alterations in neuronal cholesterol homeostasis. Depleting cholesterol in control neurons reproduced core depression-associated neuronal phenotypes, while cholesterol supplementation was sufficient to rescue these phenotypes in depression-associated conditions. These findings point to cholesterol imbalance as a common driver of neuronal dysfunction in MDD, linking diverse genetic and environmental risk factors through a shared cellular pathway.","PeriodicalId":11157,"journal":{"name":"Developmental cell","volume":" ","pages":""},"PeriodicalIF":8.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0